id sid tid token lemma pos cord-104231-fi8pskod 1 1 key key JJ cord-104231-fi8pskod 2 1 : : : cord-104231-fi8pskod 2 2 cord-104231-fi8pskod cord-104231-fi8pskod CD cord-104231-fi8pskod 2 3 authors author NNS cord-104231-fi8pskod 2 4 : : : cord-104231-fi8pskod 3 1 nan nan NNP cord-104231-fi8pskod 3 2 title title NN cord-104231-fi8pskod 3 3 : : : cord-104231-fi8pskod 3 4 The the DT cord-104231-fi8pskod 3 5 TGN38 TGN38 NNP cord-104231-fi8pskod 3 6 glycoprotein glycoprotein NN cord-104231-fi8pskod 3 7 contains contain VBZ cord-104231-fi8pskod 3 8 two two CD cord-104231-fi8pskod 3 9 non non JJ cord-104231-fi8pskod 3 10 - - JJ cord-104231-fi8pskod 3 11 overlapping overlapping JJ cord-104231-fi8pskod 3 12 signals signal NNS cord-104231-fi8pskod 3 13 that that WDT cord-104231-fi8pskod 3 14 mediate mediate VBP cord-104231-fi8pskod 3 15 localization localization NN cord-104231-fi8pskod 3 16 to to IN cord-104231-fi8pskod 3 17 the the DT cord-104231-fi8pskod 3 18 trans trans NNP cord-104231-fi8pskod 3 19 - - NNP cord-104231-fi8pskod 3 20 Golgi Golgi NNP cord-104231-fi8pskod 3 21 network network NN cord-104231-fi8pskod 3 22 date date NN cord-104231-fi8pskod 3 23 : : : cord-104231-fi8pskod 3 24 1994 1994 CD cord-104231-fi8pskod 3 25 - - SYM cord-104231-fi8pskod 3 26 04 04 CD cord-104231-fi8pskod 3 27 - - HYPH cord-104231-fi8pskod 3 28 02 02 CD cord-104231-fi8pskod 3 29 journal journal NN cord-104231-fi8pskod 3 30 : : : cord-104231-fi8pskod 3 31 J J NNP cord-104231-fi8pskod 3 32 Cell Cell NNP cord-104231-fi8pskod 3 33 Biol Biol NNP cord-104231-fi8pskod 3 34 DOI DOI NNP cord-104231-fi8pskod 3 35 : : : cord-104231-fi8pskod 3 36 nan nan NNP cord-104231-fi8pskod 3 37 sha sha NNP cord-104231-fi8pskod 3 38 : : : cord-104231-fi8pskod 3 39 c40185936592afcdc0681000e25585dd58f5ef41 c40185936592afcdc0681000e25585dd58f5ef41 CD cord-104231-fi8pskod 3 40 doc_id doc_id CD cord-104231-fi8pskod 3 41 : : : cord-104231-fi8pskod 3 42 104231 104231 CD cord-104231-fi8pskod 3 43 cord_uid cord_uid NNS cord-104231-fi8pskod 3 44 : : : cord-104231-fi8pskod 3 45 fi8pskod fi8pskod . cord-104231-fi8pskod 4 1 The the DT cord-104231-fi8pskod 4 2 membrane membrane NN cord-104231-fi8pskod 4 3 - - HYPH cord-104231-fi8pskod 4 4 spanning span VBG cord-104231-fi8pskod 4 5 and and CC cord-104231-fi8pskod 4 6 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 4 7 domains domain NNS cord-104231-fi8pskod 4 8 of of IN cord-104231-fi8pskod 4 9 CD4 CD4 NNP cord-104231-fi8pskod 4 10 and and CC cord-104231-fi8pskod 4 11 CD8 CD8 NNP cord-104231-fi8pskod 4 12 were be VBD cord-104231-fi8pskod 4 13 replaced replace VBN cord-104231-fi8pskod 4 14 by by IN cord-104231-fi8pskod 4 15 those those DT cord-104231-fi8pskod 4 16 of of IN cord-104231-fi8pskod 4 17 TGN38 TGN38 NNS cord-104231-fi8pskod 4 18 . . . cord-104231-fi8pskod 5 1 After after IN cord-104231-fi8pskod 5 2 transient transient JJ cord-104231-fi8pskod 5 3 expression expression NN cord-104231-fi8pskod 5 4 in in IN cord-104231-fi8pskod 5 5 HeLa HeLa NNP cord-104231-fi8pskod 5 6 cells cell NNS cord-104231-fi8pskod 5 7 , , , cord-104231-fi8pskod 5 8 the the DT cord-104231-fi8pskod 5 9 location location NN cord-104231-fi8pskod 5 10 of of IN cord-104231-fi8pskod 5 11 the the DT cord-104231-fi8pskod 5 12 hybrid hybrid JJ cord-104231-fi8pskod 5 13 proteins protein NNS cord-104231-fi8pskod 5 14 was be VBD cord-104231-fi8pskod 5 15 determined determine VBN cord-104231-fi8pskod 5 16 using use VBG cord-104231-fi8pskod 5 17 immunofluorescence immunofluorescence NN cord-104231-fi8pskod 5 18 and and CC cord-104231-fi8pskod 5 19 quantitative quantitative JJ cord-104231-fi8pskod 5 20 immuno immuno JJ cord-104231-fi8pskod 5 21 - - HYPH cord-104231-fi8pskod 5 22 electron electron NN cord-104231-fi8pskod 5 23 microscopy microscopy NN cord-104231-fi8pskod 5 24 , , , cord-104231-fi8pskod 5 25 FACS FACS NNP cord-104231-fi8pskod 5 26 analysis analysis NN cord-104231-fi8pskod 5 27 and and CC cord-104231-fi8pskod 5 28 metabolic metabolic JJ cord-104231-fi8pskod 5 29 labeling labeling NN cord-104231-fi8pskod 5 30 . . . cord-104231-fi8pskod 6 1 The the DT cord-104231-fi8pskod 6 2 membrane membrane NN cord-104231-fi8pskod 6 3 - - HYPH cord-104231-fi8pskod 6 4 spanning span VBG cord-104231-fi8pskod 6 5 domain domain NN cord-104231-fi8pskod 6 6 was be VBD cord-104231-fi8pskod 6 7 found find VBN cord-104231-fi8pskod 6 8 to to TO cord-104231-fi8pskod 6 9 contain contain VB cord-104231-fi8pskod 6 10 a a DT cord-104231-fi8pskod 6 11 signal signal NN cord-104231-fi8pskod 6 12 that that WDT cord-104231-fi8pskod 6 13 localized localize VBN cord-104231-fi8pskod 6 14 hybrid hybrid JJ cord-104231-fi8pskod 6 15 proteins protein NNS cord-104231-fi8pskod 6 16 to to IN cord-104231-fi8pskod 6 17 the the DT cord-104231-fi8pskod 6 18 TGN TGN NNP cord-104231-fi8pskod 6 19 . . . cord-104231-fi8pskod 7 1 This this DT cord-104231-fi8pskod 7 2 was be VBD cord-104231-fi8pskod 7 3 in in IN cord-104231-fi8pskod 7 4 addition addition NN cord-104231-fi8pskod 7 5 to to IN cord-104231-fi8pskod 7 6 the the DT cord-104231-fi8pskod 7 7 signal signal NN cord-104231-fi8pskod 7 8 previously previously RB cord-104231-fi8pskod 7 9 identified identify VBN cord-104231-fi8pskod 7 10 in in IN cord-104231-fi8pskod 7 11 the the DT cord-104231-fi8pskod 7 12 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 7 13 domain domain NN cord-104231-fi8pskod 7 14 ( ( -LRB- cord-104231-fi8pskod 7 15 Bos Bos NNP cord-104231-fi8pskod 7 16 , , , cord-104231-fi8pskod 7 17 K. K. NNP cord-104231-fi8pskod 7 18 , , , cord-104231-fi8pskod 7 19 C. C. NNP cord-104231-fi8pskod 7 20 Wraight Wraight NNP cord-104231-fi8pskod 7 21 , , , cord-104231-fi8pskod 7 22 and and CC cord-104231-fi8pskod 7 23 K. K. NNP cord-104231-fi8pskod 7 24 Stanley Stanley NNP cord-104231-fi8pskod 7 25 . . . cord-104231-fi8pskod 7 26 1993 1993 CD cord-104231-fi8pskod 7 27 . . . cord-104231-fi8pskod 8 1 EMBO EMBO NNP cord-104231-fi8pskod 9 1 ( ( -LRB- cord-104231-fi8pskod 9 2 Eur Eur NNP cord-104231-fi8pskod 9 3 . . . cord-104231-fi8pskod 9 4 Mol Mol NNP cord-104231-fi8pskod 9 5 . . . cord-104231-fi8pskod 10 1 Biol Biol NNP cord-104231-fi8pskod 10 2 . . . cord-104231-fi8pskod 11 1 Organ organ NN cord-104231-fi8pskod 11 2 ) ) -RRB- cord-104231-fi8pskod 12 1 J. J. NNP cord-104231-fi8pskod 12 2 12:2219 12:2219 CD cord-104231-fi8pskod 12 3 - - HYPH cord-104231-fi8pskod 12 4 2228 2228 CD cord-104231-fi8pskod 12 5 . . . cord-104231-fi8pskod 13 1 Humphrey Humphrey NNP cord-104231-fi8pskod 13 2 , , , cord-104231-fi8pskod 13 3 J. J. NNP cord-104231-fi8pskod 13 4 S. S. NNP cord-104231-fi8pskod 13 5 , , , cord-104231-fi8pskod 13 6 P. P. NNP cord-104231-fi8pskod 13 7 J. J. NNP cord-104231-fi8pskod 13 8 Peters Peters NNP cord-104231-fi8pskod 13 9 , , , cord-104231-fi8pskod 13 10 L. L. NNP cord-104231-fi8pskod 13 11 C. C. NNP cord-104231-fi8pskod 13 12 Yuan Yuan NNP cord-104231-fi8pskod 13 13 , , , cord-104231-fi8pskod 13 14 and and CC cord-104231-fi8pskod 13 15 J. J. NNP cord-104231-fi8pskod 13 16 S. S. NNP cord-104231-fi8pskod 13 17 Bonifacino Bonifacino NNP cord-104231-fi8pskod 13 18 . . . cord-104231-fi8pskod 13 19 1993 1993 CD cord-104231-fi8pskod 13 20 . . . cord-104231-fi8pskod 14 1 J. J. NNP cord-104231-fi8pskod 14 2 Cell Cell NNP cord-104231-fi8pskod 14 3 Biol Biol NNP cord-104231-fi8pskod 14 4 . . . cord-104231-fi8pskod 15 1 120:1123 120:1123 CD cord-104231-fi8pskod 15 2 - - HYPH cord-104231-fi8pskod 15 3 1135 1135 CD cord-104231-fi8pskod 15 4 . . . cord-104231-fi8pskod 16 1 Wong Wong NNP cord-104231-fi8pskod 16 2 , , , cord-104231-fi8pskod 16 3 S. S. NNP cord-104231-fi8pskod 16 4 H. H. NNP cord-104231-fi8pskod 16 5 , , , cord-104231-fi8pskod 16 6 and and CC cord-104231-fi8pskod 16 7 W. W. NNP cord-104231-fi8pskod 16 8 Hong Hong NNP cord-104231-fi8pskod 16 9 . . . cord-104231-fi8pskod 17 1 1993 1993 CD cord-104231-fi8pskod 17 2 . . . cord-104231-fi8pskod 18 1 J. J. NNP cord-104231-fi8pskod 18 2 Biol Biol NNP cord-104231-fi8pskod 18 3 . . . cord-104231-fi8pskod 19 1 Chem Chem NNP cord-104231-fi8pskod 19 2 . . . cord-104231-fi8pskod 20 1 268:22853 268:22853 NNP cord-104231-fi8pskod 20 2 - - HYPH cord-104231-fi8pskod 20 3 22862 22862 CD cord-104231-fi8pskod 20 4 ) ) -RRB- cord-104231-fi8pskod 20 5 . . . cord-104231-fi8pskod 21 1 The the DT cord-104231-fi8pskod 21 2 different different JJ cord-104231-fi8pskod 21 3 properties property NNS cord-104231-fi8pskod 21 4 of of IN cord-104231-fi8pskod 21 5 these these DT cord-104231-fi8pskod 21 6 two two CD cord-104231-fi8pskod 21 7 signals signal NNS cord-104231-fi8pskod 21 8 suggest suggest VBP cord-104231-fi8pskod 21 9 that that IN cord-104231-fi8pskod 21 10 each each DT cord-104231-fi8pskod 21 11 operates operate VBZ cord-104231-fi8pskod 21 12 by by IN cord-104231-fi8pskod 21 13 a a DT cord-104231-fi8pskod 21 14 different different JJ cord-104231-fi8pskod 21 15 mechanism mechanism NN cord-104231-fi8pskod 21 16 . . . cord-104231-fi8pskod 22 1 motif motif NN cord-104231-fi8pskod 22 2 results result VBZ cord-104231-fi8pskod 22 3 in in IN cord-104231-fi8pskod 22 4 the the DT cord-104231-fi8pskod 22 5 retrieval retrieval NN cord-104231-fi8pskod 22 6 of of IN cord-104231-fi8pskod 22 7 soluble soluble JJ cord-104231-fi8pskod 22 8 ER ER NNP cord-104231-fi8pskod 22 9 proteins protein NNS cord-104231-fi8pskod 22 10 lost lose VBN cord-104231-fi8pskod 22 11 from from IN cord-104231-fi8pskod 22 12 this this DT cord-104231-fi8pskod 22 13 compartment compartment NN cord-104231-fi8pskod 22 14 ( ( -LRB- cord-104231-fi8pskod 22 15 Munro Munro NNP cord-104231-fi8pskod 22 16 and and CC cord-104231-fi8pskod 22 17 Pelham Pelham NNP cord-104231-fi8pskod 22 18 , , , cord-104231-fi8pskod 22 19 1987 1987 CD cord-104231-fi8pskod 22 20 ) ) -RRB- cord-104231-fi8pskod 22 21 . . . cord-104231-fi8pskod 23 1 This this DT cord-104231-fi8pskod 23 2 sequence sequence NN cord-104231-fi8pskod 23 3 is be VBZ cord-104231-fi8pskod 23 4 recognized recognize VBN cord-104231-fi8pskod 23 5 by by IN cord-104231-fi8pskod 23 6 a a DT cord-104231-fi8pskod 23 7 receptor receptor NN cord-104231-fi8pskod 23 8 which which WDT cord-104231-fi8pskod 23 9 returns return VBZ cord-104231-fi8pskod 23 10 the the DT cord-104231-fi8pskod 23 11 protein protein NN cord-104231-fi8pskod 23 12 to to IN cord-104231-fi8pskod 23 13 the the DT cord-104231-fi8pskod 23 14 ER er NN cord-104231-fi8pskod 24 1 ( ( -LRB- cord-104231-fi8pskod 24 2 Semenza Semenza NNP cord-104231-fi8pskod 24 3 et et NNP cord-104231-fi8pskod 24 4 al al NNP cord-104231-fi8pskod 24 5 . . . cord-104231-fi8pskod 25 1 , , , cord-104231-fi8pskod 25 2 1990 1990 CD cord-104231-fi8pskod 25 3 ; ; : cord-104231-fi8pskod 25 4 Lewis Lewis NNP cord-104231-fi8pskod 25 5 and and CC cord-104231-fi8pskod 25 6 Pelham Pelham NNP cord-104231-fi8pskod 25 7 , , , cord-104231-fi8pskod 25 8 1992 1992 CD cord-104231-fi8pskod 25 9 ) ) -RRB- cord-104231-fi8pskod 25 10 . . . cord-104231-fi8pskod 26 1 Some some DT cord-104231-fi8pskod 26 2 membrane membrane NN cord-104231-fi8pskod 26 3 proteins protein NNS cord-104231-fi8pskod 26 4 contain contain VBP cord-104231-fi8pskod 26 5 a a DT cord-104231-fi8pskod 26 6 double double JJ cord-104231-fi8pskod 26 7 - - HYPH cord-104231-fi8pskod 26 8 lysine lysine NN cord-104231-fi8pskod 26 9 motif motif NN cord-104231-fi8pskod 26 10 in in IN cord-104231-fi8pskod 26 11 the the DT cord-104231-fi8pskod 26 12 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 26 13 tail tail NN cord-104231-fi8pskod 26 14 which which WDT cord-104231-fi8pskod 26 15 ensures ensure VBZ cord-104231-fi8pskod 26 16 retrieval retrieval NN cord-104231-fi8pskod 26 17 to to IN cord-104231-fi8pskod 26 18 the the DT cord-104231-fi8pskod 26 19 ER ER NNP cord-104231-fi8pskod 26 20 ( ( -LRB- cord-104231-fi8pskod 26 21 Jackson Jackson NNP cord-104231-fi8pskod 26 22 et et NNP cord-104231-fi8pskod 26 23 al al NNP cord-104231-fi8pskod 26 24 . . NNP cord-104231-fi8pskod 26 25 , , , cord-104231-fi8pskod 26 26 1993 1993 CD cord-104231-fi8pskod 26 27 ) ) -RRB- cord-104231-fi8pskod 26 28 . . . cord-104231-fi8pskod 27 1 Golgi Golgi NNP cord-104231-fi8pskod 27 2 retention retention NN cord-104231-fi8pskod 27 3 signals signal NNS cord-104231-fi8pskod 27 4 were be VBD cord-104231-fi8pskod 27 5 first first RB cord-104231-fi8pskod 27 6 identified identify VBN cord-104231-fi8pskod 27 7 in in IN cord-104231-fi8pskod 27 8 a a DT cord-104231-fi8pskod 27 9 viral viral JJ cord-104231-fi8pskod 27 10 protein protein NN cord-104231-fi8pskod 27 11 ( ( -LRB- cord-104231-fi8pskod 27 12 Swift Swift NNP cord-104231-fi8pskod 27 13 and and CC cord-104231-fi8pskod 27 14 Machamer Machamer NNP cord-104231-fi8pskod 27 15 , , , cord-104231-fi8pskod 27 16 1991 1991 CD cord-104231-fi8pskod 27 17 ) ) -RRB- cord-104231-fi8pskod 27 18 and and CC cord-104231-fi8pskod 27 19 in in IN cord-104231-fi8pskod 27 20 Golgi Golgi NNP cord-104231-fi8pskod 27 21 glycosylation glycosylation NN cord-104231-fi8pskod 27 22 enzymes enzyme NNS cord-104231-fi8pskod 27 23 ( ( -LRB- cord-104231-fi8pskod 27 24 Nilsson Nilsson NNP cord-104231-fi8pskod 27 25 et et NNP cord-104231-fi8pskod 27 26 al al NNP cord-104231-fi8pskod 27 27 . . NNP cord-104231-fi8pskod 27 28 , , , cord-104231-fi8pskod 27 29 1991 1991 CD cord-104231-fi8pskod 27 30 ; ; : cord-104231-fi8pskod 27 31 Munro Munro NNP cord-104231-fi8pskod 27 32 , , , cord-104231-fi8pskod 27 33 1991 1991 CD cord-104231-fi8pskod 27 34 ; ; : cord-104231-fi8pskod 27 35 Aoki Aoki NNP cord-104231-fi8pskod 27 36 et et NNP cord-104231-fi8pskod 27 37 al al NNP cord-104231-fi8pskod 27 38 . . NNP cord-104231-fi8pskod 27 39 , , , cord-104231-fi8pskod 27 40 1992 1992 CD cord-104231-fi8pskod 27 41 ; ; : cord-104231-fi8pskod 27 42 Burke Burke NNP cord-104231-fi8pskod 27 43 et et NNP cord-104231-fi8pskod 27 44 al al NNP cord-104231-fi8pskod 27 45 . . NNP cord-104231-fi8pskod 27 46 , , , cord-104231-fi8pskod 27 47 1992 1992 CD cord-104231-fi8pskod 27 48 ; ; : cord-104231-fi8pskod 27 49 Colley Colley NNP cord-104231-fi8pskod 27 50 et et NNP cord-104231-fi8pskod 27 51 al al NNP cord-104231-fi8pskod 27 52 . . NNP cord-104231-fi8pskod 27 53 , , , cord-104231-fi8pskod 27 54 1992 1992 CD cord-104231-fi8pskod 27 55 ; ; : cord-104231-fi8pskod 27 56 Tang Tang NNP cord-104231-fi8pskod 27 57 et et NNP cord-104231-fi8pskod 27 58 al al NNP cord-104231-fi8pskod 27 59 . . NNP cord-104231-fi8pskod 27 60 , , , cord-104231-fi8pskod 27 61 1992 1992 CD cord-104231-fi8pskod 27 62 ; ; : cord-104231-fi8pskod 27 63 Teasdale Teasdale NNP cord-104231-fi8pskod 27 64 et et NNP cord-104231-fi8pskod 27 65 al al NNP cord-104231-fi8pskod 27 66 . . NNP cord-104231-fi8pskod 27 67 , , , cord-104231-fi8pskod 27 68 1992 1992 CD cord-104231-fi8pskod 27 69 ; ; : cord-104231-fi8pskod 27 70 Wong Wong NNP cord-104231-fi8pskod 27 71 et et NNP cord-104231-fi8pskod 27 72 al al NNP cord-104231-fi8pskod 27 73 . . NNP cord-104231-fi8pskod 27 74 , , , cord-104231-fi8pskod 27 75 1992 1992 CD cord-104231-fi8pskod 27 76 ) ) -RRB- cord-104231-fi8pskod 27 77 . . . cord-104231-fi8pskod 28 1 In in IN cord-104231-fi8pskod 28 2 all all DT cord-104231-fi8pskod 28 3 cases case NNS cord-104231-fi8pskod 28 4 , , , cord-104231-fi8pskod 28 5 the the DT cord-104231-fi8pskod 28 6 retention retention NN cord-104231-fi8pskod 28 7 signal signal NN cord-104231-fi8pskod 28 8 lies lie VBZ cord-104231-fi8pskod 28 9 in in IN cord-104231-fi8pskod 28 10 the the DT cord-104231-fi8pskod 28 11 membrane membrane NN cord-104231-fi8pskod 28 12 - - HYPH cord-104231-fi8pskod 28 13 spanning span VBG cord-104231-fi8pskod 28 14 domain domain NN cord-104231-fi8pskod 28 15 . . . cord-104231-fi8pskod 29 1 More more RBR cord-104231-fi8pskod 29 2 recently recently RB cord-104231-fi8pskod 29 3 , , , cord-104231-fi8pskod 29 4 membrane membrane NN cord-104231-fi8pskod 29 5 - - HYPH cord-104231-fi8pskod 29 6 spanning span VBG cord-104231-fi8pskod 29 7 domains domain NNS cord-104231-fi8pskod 29 8 have have VBP cord-104231-fi8pskod 29 9 been be VBN cord-104231-fi8pskod 29 10 implicated implicate VBN cord-104231-fi8pskod 29 11 in in IN cord-104231-fi8pskod 29 12 the the DT cord-104231-fi8pskod 29 13 localization localization NN cord-104231-fi8pskod 29 14 of of IN cord-104231-fi8pskod 29 15 two two CD cord-104231-fi8pskod 29 16 proteins protein NNS cord-104231-fi8pskod 29 17 to to IN cord-104231-fi8pskod 29 18 the the DT cord-104231-fi8pskod 29 19 nuclear nuclear JJ cord-104231-fi8pskod 29 20 envelope envelope NN cord-104231-fi8pskod 29 21 ( ( -LRB- cord-104231-fi8pskod 29 22 Wozniak Wozniak NNP cord-104231-fi8pskod 29 23 and and CC cord-104231-fi8pskod 29 24 Blobel Blobel NNP cord-104231-fi8pskod 29 25 , , , cord-104231-fi8pskod 29 26 1992 1992 CD cord-104231-fi8pskod 29 27 ; ; : cord-104231-fi8pskod 29 28 Smith Smith NNP cord-104231-fi8pskod 29 29 and and CC cord-104231-fi8pskod 29 30 Blobel Blobel NNP cord-104231-fi8pskod 29 31 , , , cord-104231-fi8pskod 29 32 1993 1993 CD cord-104231-fi8pskod 29 33 ) ) -RRB- cord-104231-fi8pskod 29 34 . . . cord-104231-fi8pskod 30 1 So so RB cord-104231-fi8pskod 30 2 far far RB cord-104231-fi8pskod 30 3 , , , cord-104231-fi8pskod 30 4 none none NN cord-104231-fi8pskod 30 5 of of IN cord-104231-fi8pskod 30 6 the the DT cord-104231-fi8pskod 30 7 proteins protein NNS cord-104231-fi8pskod 30 8 studied study VBN cord-104231-fi8pskod 30 9 have have VBP cord-104231-fi8pskod 30 10 been be VBN cord-104231-fi8pskod 30 11 shown show VBN cord-104231-fi8pskod 30 12 to to TO cord-104231-fi8pskod 30 13 contain contain VB cord-104231-fi8pskod 30 14 both both CC cord-104231-fi8pskod 30 15 a a DT cord-104231-fi8pskod 30 16 retention retention NN cord-104231-fi8pskod 30 17 and and CC cord-104231-fi8pskod 30 18 a a DT cord-104231-fi8pskod 30 19 retrieval retrieval NN cord-104231-fi8pskod 30 20 signal signal NN cord-104231-fi8pskod 30 21 though though IN cord-104231-fi8pskod 30 22 there there EX cord-104231-fi8pskod 30 23 are be VBP cord-104231-fi8pskod 30 24 indications indication NNS cord-104231-fi8pskod 30 25 that that IN cord-104231-fi8pskod 30 26 more more JJR cord-104231-fi8pskod 30 27 than than IN cord-104231-fi8pskod 30 28 one one CD cord-104231-fi8pskod 30 29 signal signal NN cord-104231-fi8pskod 30 30 is be VBZ cord-104231-fi8pskod 30 31 present present JJ cord-104231-fi8pskod 30 32 in in IN cord-104231-fi8pskod 30 33 some some DT cord-104231-fi8pskod 30 34 of of IN cord-104231-fi8pskod 30 35 them -PRON- PRP cord-104231-fi8pskod 30 36 . . . cord-104231-fi8pskod 31 1 When when WRB cord-104231-fi8pskod 31 2 the the DT cord-104231-fi8pskod 31 3 carboxy carboxy JJ cord-104231-fi8pskod 31 4 - - HYPH cord-104231-fi8pskod 31 5 terminal terminal NN cord-104231-fi8pskod 31 6 -KDEL -KDEL HYPH cord-104231-fi8pskod 31 7 signal signal NN cord-104231-fi8pskod 31 8 is be VBZ cord-104231-fi8pskod 31 9 removed remove VBN cord-104231-fi8pskod 31 10 from from IN cord-104231-fi8pskod 31 11 the the DT cord-104231-fi8pskod 31 12 soluble soluble JJ cord-104231-fi8pskod 31 13 BiP BiP NNP cord-104231-fi8pskod 31 14 protein protein NN cord-104231-fi8pskod 31 15 , , , cord-104231-fi8pskod 31 16 it -PRON- PRP cord-104231-fi8pskod 31 17 is be VBZ cord-104231-fi8pskod 31 18 secreted secrete VBN cord-104231-fi8pskod 31 19 very very RB cord-104231-fi8pskod 31 20 slowly slowly RB cord-104231-fi8pskod 31 21 ( ( -LRB- cord-104231-fi8pskod 31 22 Munro Munro NNP cord-104231-fi8pskod 31 23 and and CC cord-104231-fi8pskod 31 24 Pelham Pelham NNP cord-104231-fi8pskod 31 25 , , , cord-104231-fi8pskod 31 26 1987 1987 CD cord-104231-fi8pskod 31 27 ) ) -RRB- cord-104231-fi8pskod 31 28 . . . cord-104231-fi8pskod 32 1 When when WRB cord-104231-fi8pskod 32 2 the the DT cord-104231-fi8pskod 32 3 double double JJ cord-104231-fi8pskod 32 4 - - HYPH cord-104231-fi8pskod 32 5 lysine lysine NN cord-104231-fi8pskod 32 6 motif motif NN cord-104231-fi8pskod 32 7 is be VBZ cord-104231-fi8pskod 32 8 removed remove VBN cord-104231-fi8pskod 32 9 from from IN cord-104231-fi8pskod 32 10 glucuronosyltransferase glucuronosyltransferase NNP cord-104231-fi8pskod 32 11 , , , cord-104231-fi8pskod 32 12 an an DT cord-104231-fi8pskod 32 13 integral integral JJ cord-104231-fi8pskod 32 14 membrane membrane NN cord-104231-fi8pskod 32 15 protein protein NN cord-104231-fi8pskod 32 16 , , , cord-104231-fi8pskod 32 17 little little JJ cord-104231-fi8pskod 32 18 if if IN cord-104231-fi8pskod 32 19 any any DT cord-104231-fi8pskod 32 20 escapes escape NNS cord-104231-fi8pskod 32 21 from from IN cord-104231-fi8pskod 32 22 the the DT cord-104231-fi8pskod 32 23 ER ER NNP cord-104231-fi8pskod 33 1 ( ( -LRB- cord-104231-fi8pskod 33 2 Jackson Jackson NNP cord-104231-fi8pskod 33 3 et et NNP cord-104231-fi8pskod 33 4 al al NNP cord-104231-fi8pskod 33 5 . . . cord-104231-fi8pskod 34 1 , , , cord-104231-fi8pskod 34 2 1993 1993 CD cord-104231-fi8pskod 34 3 ) ) -RRB- cord-104231-fi8pskod 34 4 . . . cord-104231-fi8pskod 35 1 Both both CC cord-104231-fi8pskod 35 2 these these DT cord-104231-fi8pskod 35 3 cases case NNS cord-104231-fi8pskod 35 4 suggests suggest VBZ cord-104231-fi8pskod 35 5 that that IN cord-104231-fi8pskod 35 6 a a DT cord-104231-fi8pskod 35 7 retention retention NN cord-104231-fi8pskod 35 8 signal signal NN cord-104231-fi8pskod 35 9 is be VBZ cord-104231-fi8pskod 35 10 present present JJ cord-104231-fi8pskod 35 11 in in IN cord-104231-fi8pskod 35 12 addition addition NN cord-104231-fi8pskod 35 13 to to IN cord-104231-fi8pskod 35 14 the the DT cord-104231-fi8pskod 35 15 retrieval retrieval NN cord-104231-fi8pskod 35 16 signal signal NN cord-104231-fi8pskod 35 17 . . . cord-104231-fi8pskod 36 1 To to TO cord-104231-fi8pskod 36 2 determine determine VB cord-104231-fi8pskod 36 3 whether whether IN cord-104231-fi8pskod 36 4 a a DT cord-104231-fi8pskod 36 5 protein protein NN cord-104231-fi8pskod 36 6 can can MD cord-104231-fi8pskod 36 7 contain contain VB cord-104231-fi8pskod 36 8 both both DT cord-104231-fi8pskod 36 9 signals signal NNS cord-104231-fi8pskod 36 10 , , , cord-104231-fi8pskod 36 11 we -PRON- PRP cord-104231-fi8pskod 36 12 focused focus VBD cord-104231-fi8pskod 36 13 on on IN cord-104231-fi8pskod 36 14 TGN38 TGN38 NNP cord-104231-fi8pskod 36 15 , , , cord-104231-fi8pskod 36 16 a a DT cord-104231-fi8pskod 36 17 resident resident NN cord-104231-fi8pskod 36 18 of of IN cord-104231-fi8pskod 36 19 the the DT cord-104231-fi8pskod 36 20 trans trans NNP cord-104231-fi8pskod 36 21 - - NNP cord-104231-fi8pskod 36 22 Golgi Golgi NNP cord-104231-fi8pskod 36 23 network network NN cord-104231-fi8pskod 36 24 ( ( -LRB- cord-104231-fi8pskod 36 25 Luzio Luzio NNP cord-104231-fi8pskod 36 26 et et FW cord-104231-fi8pskod 36 27 al al NNP cord-104231-fi8pskod 36 28 . . NNP cord-104231-fi8pskod 36 29 , , , cord-104231-fi8pskod 36 30 1990 1990 CD cord-104231-fi8pskod 36 31 ) ) -RRB- cord-104231-fi8pskod 36 32 . . . cord-104231-fi8pskod 37 1 TGN38 TGN38 NNP cord-104231-fi8pskod 37 2 has have VBZ cord-104231-fi8pskod 37 3 a a DT cord-104231-fi8pskod 37 4 tyrosine tyrosine NN cord-104231-fi8pskod 37 5 - - HYPH cord-104231-fi8pskod 37 6 based base VBN cord-104231-fi8pskod 37 7 motif motif NN cord-104231-fi8pskod 37 8 in in IN cord-104231-fi8pskod 37 9 the the DT cord-104231-fi8pskod 37 10 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 37 11 domain domain NN cord-104231-fi8pskod 37 12 that that WDT cord-104231-fi8pskod 37 13 acts act VBZ cord-104231-fi8pskod 37 14 as as IN cord-104231-fi8pskod 37 15 a a DT cord-104231-fi8pskod 37 16 retrieval retrieval NN cord-104231-fi8pskod 37 17 signal signal NN cord-104231-fi8pskod 37 18 , , , cord-104231-fi8pskod 37 19 return return NN cord-104231-fi8pskod 37 20 - - HYPH cord-104231-fi8pskod 37 21 ing e VBG cord-104231-fi8pskod 37 22 the the DT cord-104231-fi8pskod 37 23 protein protein NN cord-104231-fi8pskod 37 24 from from IN cord-104231-fi8pskod 37 25 the the DT cord-104231-fi8pskod 37 26 cell cell NN cord-104231-fi8pskod 37 27 surface surface NN cord-104231-fi8pskod 37 28 to to IN cord-104231-fi8pskod 37 29 the the DT cord-104231-fi8pskod 37 30 TGN TGN NNP cord-104231-fi8pskod 37 31 ( ( -LRB- cord-104231-fi8pskod 37 32 Bos Bos NNP cord-104231-fi8pskod 37 33 et et NNP cord-104231-fi8pskod 37 34 al al NNP cord-104231-fi8pskod 37 35 . . NNP cord-104231-fi8pskod 37 36 , , , cord-104231-fi8pskod 37 37 1993 1993 CD cord-104231-fi8pskod 37 38 ; ; : cord-104231-fi8pskod 37 39 Humphrey Humphrey NNP cord-104231-fi8pskod 37 40 et et NNP cord-104231-fi8pskod 37 41 al al NNP cord-104231-fi8pskod 37 42 . . NNP cord-104231-fi8pskod 37 43 , , , cord-104231-fi8pskod 37 44 1993 1993 CD cord-104231-fi8pskod 37 45 ; ; : cord-104231-fi8pskod 38 1 Wong Wong NNP cord-104231-fi8pskod 38 2 and and CC cord-104231-fi8pskod 38 3 Hong Hong NNP cord-104231-fi8pskod 38 4 , , , cord-104231-fi8pskod 38 5 1993 1993 CD cord-104231-fi8pskod 38 6 ) ) -RRB- cord-104231-fi8pskod 38 7 . . . cord-104231-fi8pskod 39 1 Here here RB cord-104231-fi8pskod 39 2 we -PRON- PRP cord-104231-fi8pskod 39 3 have have VBP cord-104231-fi8pskod 39 4 looked look VBN cord-104231-fi8pskod 39 5 for for IN cord-104231-fi8pskod 39 6 the the DT cord-104231-fi8pskod 39 7 second second JJ cord-104231-fi8pskod 39 8 signal signal NN cord-104231-fi8pskod 39 9 and and CC cord-104231-fi8pskod 39 10 have have VBP cord-104231-fi8pskod 39 11 found find VBN cord-104231-fi8pskod 39 12 it -PRON- PRP cord-104231-fi8pskod 39 13 in in IN cord-104231-fi8pskod 39 14 the the DT cord-104231-fi8pskod 39 15 membrane membrane NN cord-104231-fi8pskod 39 16 - - HYPH cord-104231-fi8pskod 39 17 spanning span VBG cord-104231-fi8pskod 39 18 domain domain NN cord-104231-fi8pskod 39 19 . . . cord-104231-fi8pskod 40 1 Chemicals chemical NNS cord-104231-fi8pskod 40 2 and and CC cord-104231-fi8pskod 40 3 reagents reagent NNS cord-104231-fi8pskod 40 4 were be VBD cord-104231-fi8pskod 40 5 obtained obtain VBN cord-104231-fi8pskod 40 6 from from IN cord-104231-fi8pskod 40 7 the the DT cord-104231-fi8pskod 40 8 sources source NNS cord-104231-fi8pskod 40 9 described describe VBN cord-104231-fi8pskod 40 10 by by IN cord-104231-fi8pskod 40 11 Nilsson Nilsson NNP cord-104231-fi8pskod 40 12 et et NNP cord-104231-fi8pskod 40 13 al al NNP cord-104231-fi8pskod 40 14 . . . cord-104231-fi8pskod 41 1 ( ( -LRB- cord-104231-fi8pskod 41 2 1993a 1993a CD cord-104231-fi8pskod 41 3 Nilsson Nilsson NNP cord-104231-fi8pskod 41 4 et et NNP cord-104231-fi8pskod 41 5 al al NNP cord-104231-fi8pskod 41 6 . . . cord-104231-fi8pskod 42 1 ( ( -LRB- cord-104231-fi8pskod 42 2 , , , cord-104231-fi8pskod 42 3 1994 1994 CD cord-104231-fi8pskod 42 4 . . . cord-104231-fi8pskod 43 1 Restriction restriction NN cord-104231-fi8pskod 43 2 enzymes enzyme NNS cord-104231-fi8pskod 43 3 , , , cord-104231-fi8pskod 43 4 T4 T4 NNP cord-104231-fi8pskod 43 5 DNA DNA NNP cord-104231-fi8pskod 43 6 ligase ligase NN cord-104231-fi8pskod 43 7 , , , cord-104231-fi8pskod 43 8 and and CC cord-104231-fi8pskod 43 9 polynucleotide polynucleotide NN cord-104231-fi8pskod 43 10 kinase kinase NN cord-104231-fi8pskod 43 11 for for IN cord-104231-fi8pskod 43 12 recombinant recombinant JJ cord-104231-fi8pskod 43 13 DNA dna NN cord-104231-fi8pskod 43 14 procedures procedure NNS cord-104231-fi8pskod 43 15 were be VBD cord-104231-fi8pskod 43 16 purchased purchase VBN cord-104231-fi8pskod 43 17 from from IN cord-104231-fi8pskod 43 18 GIBCO GIBCO NNP cord-104231-fi8pskod 43 19 BRL BRL NNP cord-104231-fi8pskod 43 20 ( ( -LRB- cord-104231-fi8pskod 43 21 Gaithershurg Gaithershurg NNP cord-104231-fi8pskod 43 22 , , , cord-104231-fi8pskod 43 23 MD MD NNP cord-104231-fi8pskod 43 24 ) ) -RRB- cord-104231-fi8pskod 43 25 , , . cord-104231-fi8pskod 44 1 New New NNP cord-104231-fi8pskod 44 2 England England NNP cord-104231-fi8pskod 44 3 Biolahs Biolahs NNP cord-104231-fi8pskod 44 4 ( ( -LRB- cord-104231-fi8pskod 44 5 Bethesda Bethesda NNP cord-104231-fi8pskod 44 6 , , , cord-104231-fi8pskod 44 7 MD MD NNP cord-104231-fi8pskod 44 8 ) ) -RRB- cord-104231-fi8pskod 44 9 , , , cord-104231-fi8pskod 44 10 or or CC cord-104231-fi8pskod 44 11 Pharmacia Pharmacia NNP cord-104231-fi8pskod 44 12 Corp. Corp. NNP cord-104231-fi8pskod 44 13 ( ( -LRB- cord-104231-fi8pskod 44 14 Uppsala Uppsala NNP cord-104231-fi8pskod 44 15 , , , cord-104231-fi8pskod 44 16 Sweden Sweden NNP cord-104231-fi8pskod 44 17 ) ) -RRB- cord-104231-fi8pskod 44 18 . . . cord-104231-fi8pskod 45 1 Sequencing sequencing NN cord-104231-fi8pskod 45 2 reagents reagent NNS cord-104231-fi8pskod 45 3 and and CC cord-104231-fi8pskod 45 4 T7 t7 NN cord-104231-fi8pskod 45 5 polymerase polymerase NN cord-104231-fi8pskod 45 6 were be VBD cord-104231-fi8pskod 45 7 purchased purchase VBN cord-104231-fi8pskod 45 8 from from IN cord-104231-fi8pskod 45 9 USB usb NN cord-104231-fi8pskod 45 10 Biochemicals biochemical NNS cord-104231-fi8pskod 45 11 . . . cord-104231-fi8pskod 46 1 Cell cell NN cord-104231-fi8pskod 46 2 culture culture NN cord-104231-fi8pskod 46 3 media medium NNS cord-104231-fi8pskod 46 4 , , , cord-104231-fi8pskod 46 5 fetal fetal JJ cord-104231-fi8pskod 46 6 calf calf NN cord-104231-fi8pskod 46 7 serum serum NN cord-104231-fi8pskod 46 8 , , , cord-104231-fi8pskod 46 9 amino amino JJ cord-104231-fi8pskod 46 10 acids acid NNS cord-104231-fi8pskod 46 11 , , , cord-104231-fi8pskod 46 12 and and CC cord-104231-fi8pskod 46 13 penicillin penicillin NN cord-104231-fi8pskod 46 14 / / SYM cord-104231-fi8pskod 46 15 streptomycin streptomycin NNS cord-104231-fi8pskod 46 16 were be VBD cord-104231-fi8pskod 46 17 from from IN cord-104231-fi8pskod 46 18 GIBCO GIBCO NNP cord-104231-fi8pskod 46 19 BRL BRL NNP cord-104231-fi8pskod 46 20 . . . cord-104231-fi8pskod 46 21 _SP cord-104231-fi8pskod 47 1 The the DT cord-104231-fi8pskod 47 2 TGN38 TGN38 NNP cord-104231-fi8pskod 47 3 eDNA edna RB cord-104231-fi8pskod 48 1 ( ( -LRB- cord-104231-fi8pskod 48 2 Lnzio Lnzio NNP cord-104231-fi8pskod 48 3 et et FW cord-104231-fi8pskod 48 4 al al NNP cord-104231-fi8pskod 48 5 . . NNP cord-104231-fi8pskod 48 6 , , , cord-104231-fi8pskod 48 7 1990 1990 CD cord-104231-fi8pskod 48 8 ) ) -RRB- cord-104231-fi8pskod 48 9 was be VBD cord-104231-fi8pskod 48 10 mutagenized mutagenize VBN cord-104231-fi8pskod 48 11 by by IN cord-104231-fi8pskod 48 12 PCR PCR NNP cord-104231-fi8pskod 49 1 such such JJ cord-104231-fi8pskod 49 2 that that IN cord-104231-fi8pskod 49 3 a a DT cord-104231-fi8pskod 49 4 BamHI bamhi NN cord-104231-fi8pskod 49 5 site site NN cord-104231-fi8pskod 49 6 was be VBD cord-104231-fi8pskod 49 7 introduced introduce VBN cord-104231-fi8pskod 49 8 just just RB cord-104231-fi8pskod 49 9 after after IN cord-104231-fi8pskod 49 10 the the DT cord-104231-fi8pskod 49 11 stop stop NN cord-104231-fi8pskod 49 12 codon codon NN cord-104231-fi8pskod 49 13 . . . cord-104231-fi8pskod 50 1 The the DT cord-104231-fi8pskod 50 2 HindIII HindIII NNP cord-104231-fi8pskod 50 3 - - HYPH cord-104231-fi8pskod 50 4 BamHI bamhi NN cord-104231-fi8pskod 50 5 fragment fragment NN cord-104231-fi8pskod 50 6 containing contain VBG cord-104231-fi8pskod 50 7 the the DT cord-104231-fi8pskod 50 8 5 5 CD cord-104231-fi8pskod 50 9 ' ' POS cord-104231-fi8pskod 50 10 untranslated untranslated JJ cord-104231-fi8pskod 50 11 region region NN cord-104231-fi8pskod 50 12 and and CC cord-104231-fi8pskod 50 13 the the DT cord-104231-fi8pskod 50 14 complete complete JJ cord-104231-fi8pskod 50 15 coding coding NN cord-104231-fi8pskod 50 16 sequence sequence NN cord-104231-fi8pskod 50 17 was be VBD cord-104231-fi8pskod 50 18 cloned clone VBN cord-104231-fi8pskod 50 19 between between IN cord-104231-fi8pskod 50 20 the the DT cord-104231-fi8pskod 50 21 same same JJ cord-104231-fi8pskod 50 22 restriction restriction NN cord-104231-fi8pskod 50 23 sites site NNS cord-104231-fi8pskod 50 24 in in IN cord-104231-fi8pskod 50 25 the the DT cord-104231-fi8pskod 50 26 pCMUIV pcmuiv NN cord-104231-fi8pskod 50 27 plasmid plasmid NN cord-104231-fi8pskod 50 28 ( ( -LRB- cord-104231-fi8pskod 50 29 Nilsson Nilsson NNP cord-104231-fi8pskod 50 30 et et NNP cord-104231-fi8pskod 50 31 al al NNP cord-104231-fi8pskod 50 32 . . NNP cord-104231-fi8pskod 50 33 , , , cord-104231-fi8pskod 50 34 1989 1989 CD cord-104231-fi8pskod 50 35 ) ) -RRB- cord-104231-fi8pskod 50 36 . . . cord-104231-fi8pskod 51 1 The the DT cord-104231-fi8pskod 51 2 TGN38 TGN38 NNP cord-104231-fi8pskod 51 3 eDNA edna RB cord-104231-fi8pskod 52 1 in in IN cord-104231-fi8pskod 52 2 the the DT cord-104231-fi8pskod 52 3 mammalian mammalian JJ cord-104231-fi8pskod 52 4 expression expression NN cord-104231-fi8pskod 52 5 vector vector NN cord-104231-fi8pskod 52 6 pUEX puex NN cord-104231-fi8pskod 53 1 ( ( -LRB- cord-104231-fi8pskod 53 2 Luzio Luzio NNP cord-104231-fi8pskod 53 3 et et FW cord-104231-fi8pskod 53 4 al al NNP cord-104231-fi8pskod 53 5 . . NNP cord-104231-fi8pskod 53 6 , , , cord-104231-fi8pskod 53 7 1990 1990 CD cord-104231-fi8pskod 53 8 ) ) -RRB- cord-104231-fi8pskod 53 9 was be VBD cord-104231-fi8pskod 53 10 used use VBN cord-104231-fi8pskod 53 11 as as IN cord-104231-fi8pskod 53 12 a a DT cord-104231-fi8pskod 53 13 template template NN cord-104231-fi8pskod 53 14 for for IN cord-104231-fi8pskod 53 15 PCR PCR NNP cord-104231-fi8pskod 53 16 amplification amplification NN cord-104231-fi8pskod 53 17 under under IN cord-104231-fi8pskod 53 18 the the DT cord-104231-fi8pskod 53 19 conditions condition NNS cord-104231-fi8pskod 53 20 described describe VBN cord-104231-fi8pskod 53 21 by by IN cord-104231-fi8pskod 53 22 Salki Salki NNP cord-104231-fi8pskod 53 23 et et NNP cord-104231-fi8pskod 53 24 al al NNP cord-104231-fi8pskod 53 25 . . . cord-104231-fi8pskod 54 1 ( ( -LRB- cord-104231-fi8pskod 54 2 1988 1988 CD cord-104231-fi8pskod 54 3 ) ) -RRB- cord-104231-fi8pskod 54 4 . . . cord-104231-fi8pskod 55 1 Oligonucleotides oligonucleotide NNS cord-104231-fi8pskod 55 2 used use VBN cord-104231-fi8pskod 55 3 in in IN cord-104231-fi8pskod 55 4 PCR PCR NNP cord-104231-fi8pskod 55 5 reactions reaction NNS cord-104231-fi8pskod 55 6 were be VBD cord-104231-fi8pskod 55 7 generally generally RB cord-104231-fi8pskod 55 8 synthesized synthesize VBN cord-104231-fi8pskod 55 9 with with IN cord-104231-fi8pskod 55 10 BamHI bamhi NN cord-104231-fi8pskod 55 11 flanking flanking JJ cord-104231-fi8pskod 55 12 sequences sequence NNS cord-104231-fi8pskod 55 13 to to TO cord-104231-fi8pskod 55 14 facilitate facilitate VB cord-104231-fi8pskod 55 15 easy easy JJ cord-104231-fi8pskod 55 16 cloning cloning NN cord-104231-fi8pskod 55 17 into into IN cord-104231-fi8pskod 55 18 plasmid plasmid NN cord-104231-fi8pskod 55 19 vectors vector NNS cord-104231-fi8pskod 55 20 for for IN cord-104231-fi8pskod 55 21 sequencing sequencing NN cord-104231-fi8pskod 55 22 . . . cord-104231-fi8pskod 56 1 Oligonucleotides oligonucleotide NNS cord-104231-fi8pskod 56 2 were be VBD cord-104231-fi8pskod 56 3 used use VBN cord-104231-fi8pskod 56 4 at at IN cord-104231-fi8pskod 56 5 I -PRON- PRP cord-104231-fi8pskod 56 6 mM mm CD cord-104231-fi8pskod 56 7 in in IN cord-104231-fi8pskod 56 8 PCR PCR NNP cord-104231-fi8pskod 56 9 reactions reaction NNS cord-104231-fi8pskod 56 10 . . . cord-104231-fi8pskod 57 1 PCR PCR NNP cord-104231-fi8pskod 57 2 products product NNS cord-104231-fi8pskod 57 3 were be VBD cord-104231-fi8pskod 57 4 ethanol ethanol NN cord-104231-fi8pskod 57 5 precipitated precipitate VBN cord-104231-fi8pskod 57 6 , , , cord-104231-fi8pskod 57 7 digested digest VBN cord-104231-fi8pskod 57 8 with with IN cord-104231-fi8pskod 57 9 BamHI BamHI NNP cord-104231-fi8pskod 57 10 , , , cord-104231-fi8pskod 57 11 and and CC cord-104231-fi8pskod 57 12 cloned clone VBN cord-104231-fi8pskod 57 13 into into IN cord-104231-fi8pskod 57 14 the the DT cord-104231-fi8pskod 57 15 pBS pbs JJ cord-104231-fi8pskod 57 16 plasmid plasmid NN cord-104231-fi8pskod 57 17 ( ( -LRB- cord-104231-fi8pskod 57 18 Stratagene Stratagene NNP cord-104231-fi8pskod 57 19 Corp. Corp. NNP cord-104231-fi8pskod 57 20 , , , cord-104231-fi8pskod 57 21 La La NNP cord-104231-fi8pskod 57 22 Jolla Jolla NNP cord-104231-fi8pskod 57 23 , , , cord-104231-fi8pskod 57 24 CA CA NNP cord-104231-fi8pskod 57 25 ) ) -RRB- cord-104231-fi8pskod 57 26 for for IN cord-104231-fi8pskod 57 27 sequencing sequencing NN cord-104231-fi8pskod 57 28 . . . cord-104231-fi8pskod 58 1 DNA dna NN cord-104231-fi8pskod 58 2 sequencing sequencing NN cord-104231-fi8pskod 58 3 was be VBD cord-104231-fi8pskod 58 4 carried carry VBN cord-104231-fi8pskod 58 5 out out RP cord-104231-fi8pskod 58 6 using use VBG cord-104231-fi8pskod 58 7 the the DT cord-104231-fi8pskod 58 8 dideoxy dideoxy NN cord-104231-fi8pskod 58 9 chain chain NN cord-104231-fi8pskod 58 10 termination termination NN cord-104231-fi8pskod 58 11 method method NN cord-104231-fi8pskod 58 12 and and CC cord-104231-fi8pskod 58 13 T7 t7 NN cord-104231-fi8pskod 58 14 polymerase polymerase NN cord-104231-fi8pskod 58 15 ( ( -LRB- cord-104231-fi8pskod 58 16 Tabor Tabor NNP cord-104231-fi8pskod 58 17 and and CC cord-104231-fi8pskod 58 18 Richardson Richardson NNP cord-104231-fi8pskod 58 19 , , , cord-104231-fi8pskod 58 20 1987 1987 CD cord-104231-fi8pskod 58 21 ) ) -RRB- cord-104231-fi8pskod 58 22 . . . cord-104231-fi8pskod 59 1 Plasmid Plasmid NNP cord-104231-fi8pskod 59 2 derivafives derivafive NNS cord-104231-fi8pskod 59 3 were be VBD cord-104231-fi8pskod 59 4 digested digest VBN cord-104231-fi8pskod 59 5 with with IN cord-104231-fi8pskod 59 6 the the DT cord-104231-fi8pskod 59 7 appropriate appropriate JJ cord-104231-fi8pskod 59 8 enzymes enzyme NNS cord-104231-fi8pskod 59 9 to to TO cord-104231-fi8pskod 59 10 generate generate VB cord-104231-fi8pskod 59 11 the the DT cord-104231-fi8pskod 59 12 correct correct JJ cord-104231-fi8pskod 59 13 5 5 CD cord-104231-fi8pskod 59 14 ' ' '' cord-104231-fi8pskod 59 15 and and CC cord-104231-fi8pskod 59 16 3 3 CD cord-104231-fi8pskod 59 17 ' ' POS cord-104231-fi8pskod 59 18 ends end NNS cord-104231-fi8pskod 59 19 for for IN cord-104231-fi8pskod 59 20 cloning clone VBG cord-104231-fi8pskod 59 21 and and CC cord-104231-fi8pskod 59 22 construction construction NN cord-104231-fi8pskod 59 23 of of IN cord-104231-fi8pskod 59 24 CD8 CD8 NNP cord-104231-fi8pskod 59 25 and and CC cord-104231-fi8pskod 59 26 CD4 CD4 NNP cord-104231-fi8pskod 59 27 eDNA eDNA NNP cord-104231-fi8pskod 59 28 chimeras chimera NNS cord-104231-fi8pskod 59 29 . . . cord-104231-fi8pskod 60 1 CD8 CD8 NNP cord-104231-fi8pskod 60 2 hybrid hybrid NN cord-104231-fi8pskod 60 3 proteins protein NNS cord-104231-fi8pskod 60 4 were be VBD cord-104231-fi8pskod 60 5 constructed construct VBN cord-104231-fi8pskod 60 6 such such JJ cord-104231-fi8pskod 60 7 that that IN cord-104231-fi8pskod 60 8 the the DT cord-104231-fi8pskod 60 9 first first JJ cord-104231-fi8pskod 60 10 153 153 CD cord-104231-fi8pskod 60 11 amino amino JJ cord-104231-fi8pskod 60 12 acids acid NNS cord-104231-fi8pskod 60 13 of of IN cord-104231-fi8pskod 60 14 the the DT cord-104231-fi8pskod 60 15 extracellular extracellular JJ cord-104231-fi8pskod 60 16 domain domain NN cord-104231-fi8pskod 60 17 of of IN cord-104231-fi8pskod 60 18 c c NN cord-104231-fi8pskod 60 19 ~ ~ NFP cord-104231-fi8pskod 60 20 CD8 CD8 NNP cord-104231-fi8pskod 60 21 were be VBD cord-104231-fi8pskod 60 22 preserved preserve VBN cord-104231-fi8pskod 60 23 . . . cord-104231-fi8pskod 61 1 This this DT cord-104231-fi8pskod 61 2 is be VBZ cord-104231-fi8pskod 61 3 sufficient sufficient JJ cord-104231-fi8pskod 61 4 for for IN cord-104231-fi8pskod 61 5 CD8 CD8 NNP cord-104231-fi8pskod 61 6 glycosylation glycosylation NN cord-104231-fi8pskod 61 7 and and CC cord-104231-fi8pskod 61 8 secretion secretion NN cord-104231-fi8pskod 61 9 ( ( -LRB- cord-104231-fi8pskod 61 10 ~y ~y NNP cord-104231-fi8pskod 61 11 et et NNP cord-104231-fi8pskod 61 12 al al NNP cord-104231-fi8pskod 61 13 . . NNP cord-104231-fi8pskod 61 14 , , , cord-104231-fi8pskod 61 15 1992 1992 CD cord-104231-fi8pskod 61 16 ) ) -RRB- cord-104231-fi8pskod 61 17 , , , cord-104231-fi8pskod 61 18 and and CC cord-104231-fi8pskod 61 19 should should MD cord-104231-fi8pskod 61 20 also also RB cord-104231-fi8pskod 61 21 not not RB cord-104231-fi8pskod 61 22 affect affect VB cord-104231-fi8pskod 61 23 the the DT cord-104231-fi8pskod 61 24 ability ability NN cord-104231-fi8pskod 61 25 of of IN cord-104231-fi8pskod 61 26 CD8 CD8 NNP cord-104231-fi8pskod 61 27 hybrid hybrid NN cord-104231-fi8pskod 61 28 proteins protein NNS cord-104231-fi8pskod 61 29 to to TO cord-104231-fi8pskod 61 30 form form VB cord-104231-fi8pskod 61 31 homodimers homodimer NNS cord-104231-fi8pskod 62 1 ( ( -LRB- cord-104231-fi8pskod 62 2 Leahy Leahy NNP cord-104231-fi8pskod 62 3 et et NNP cord-104231-fi8pskod 62 4 al al NNP cord-104231-fi8pskod 62 5 . . . cord-104231-fi8pskod 63 1 , , , cord-104231-fi8pskod 63 2 1992 1992 CD cord-104231-fi8pskod 63 3 ; ; : cord-104231-fi8pskod 63 4 Boursier Boursier NNP cord-104231-fi8pskod 63 5 et et NNP cord-104231-fi8pskod 63 6 al al NNP cord-104231-fi8pskod 63 7 . . NNP cord-104231-fi8pskod 63 8 , , , cord-104231-fi8pskod 63 9 1993 1993 CD cord-104231-fi8pskod 63 10 ) ) -RRB- cord-104231-fi8pskod 63 11 . . . cord-104231-fi8pskod 64 1 PCR PCR NNP cord-104231-fi8pskod 64 2 - - HYPH cord-104231-fi8pskod 64 3 generated generate VBN cord-104231-fi8pskod 64 4 DNA dna NN cord-104231-fi8pskod 64 5 fragments fragment NNS cord-104231-fi8pskod 64 6 were be VBD cord-104231-fi8pskod 64 7 cloned clone VBN cord-104231-fi8pskod 64 8 into into IN cord-104231-fi8pskod 64 9 the the DT cord-104231-fi8pskod 64 10 pCMUIV pcmuiv NN cord-104231-fi8pskod 64 11 expression expression NN cord-104231-fi8pskod 64 12 vector vector NN cord-104231-fi8pskod 64 13 ( ( -LRB- cord-104231-fi8pskod 64 14 Nilsson Nilsson NNP cord-104231-fi8pskod 64 15 et et NNP cord-104231-fi8pskod 64 16 al al NNP cord-104231-fi8pskod 64 17 . . NNP cord-104231-fi8pskod 64 18 , , , cord-104231-fi8pskod 64 19 1989 1989 CD cord-104231-fi8pskod 64 20 ) ) -RRB- cord-104231-fi8pskod 64 21 using use VBG cord-104231-fi8pskod 64 22 3 3 CD cord-104231-fi8pskod 64 23 DNA dna NN cord-104231-fi8pskod 64 24 fragments fragment NNS cord-104231-fi8pskod 64 25 in in IN cord-104231-fi8pskod 64 26 a a DT cord-104231-fi8pskod 64 27 llgation llgation NN cord-104231-fi8pskod 64 28 . . . cord-104231-fi8pskod 65 1 To to TO cord-104231-fi8pskod 65 2 construct construct VB cord-104231-fi8pskod 65 3 the the DT cord-104231-fi8pskod 65 4 CD8-MC cd8-mc NN cord-104231-fi8pskod 65 5 and and CC cord-104231-fi8pskod 65 6 CD8-MAC CD8-MAC NNP cord-104231-fi8pskod 65 7 chimeric chimeric JJ cord-104231-fi8pskod 66 1 cDNAs cDNAs . cord-104231-fi8pskod 67 1 the the DT cord-104231-fi8pskod 67 2 llgation llgation NN cord-104231-fi8pskod 67 3 mix mix NN cord-104231-fi8pskod 67 4 contained contain VBD cord-104231-fi8pskod 67 5 ( ( -LRB- cord-104231-fi8pskod 67 6 1 1 LS cord-104231-fi8pskod 67 7 ) ) -RRB- cord-104231-fi8pskod 67 8 the the DT cord-104231-fi8pskod 67 9 HindHI HindHI NNP cord-104231-fi8pskod 67 10 - - HYPH cord-104231-fi8pskod 67 11 ApaLI apali NN cord-104231-fi8pskod 67 12 DNA dna NN cord-104231-fi8pskod 67 13 fragment fragment NN cord-104231-fi8pskod 67 14 that that WDT cord-104231-fi8pskod 67 15 encoded encode VBD cord-104231-fi8pskod 67 16 most most JJS cord-104231-fi8pskod 67 17 of of IN cord-104231-fi8pskod 67 18 the the DT cord-104231-fi8pskod 67 19 lumenal lumenal JJ cord-104231-fi8pskod 67 20 domain domain NN cord-104231-fi8pskod 67 21 of of IN cord-104231-fi8pskod 67 22 the the DT cord-104231-fi8pskod 67 23 CD8 CD8 NNP cord-104231-fi8pskod 67 24 molecule molecule NN cord-104231-fi8pskod 67 25 , , , cord-104231-fi8pskod 67 26 ( ( -LRB- cord-104231-fi8pskod 67 27 2 2 LS cord-104231-fi8pskod 67 28 ) ) -RRB- cord-104231-fi8pskod 67 29 an an DT cord-104231-fi8pskod 67 30 ApaLI apali NN cord-104231-fi8pskod 67 31 - - HYPH cord-104231-fi8pskod 67 32 BamHI bamhi NN cord-104231-fi8pskod 67 33 DNA dna NN cord-104231-fi8pskod 67 34 fragment fragment NN cord-104231-fi8pskod 67 35 that that WDT cord-104231-fi8pskod 67 36 encoded encode VBD cord-104231-fi8pskod 67 37 the the DT cord-104231-fi8pskod 67 38 membrane membrane NN cord-104231-fi8pskod 67 39 - - HYPH cord-104231-fi8pskod 67 40 spanning span VBG cord-104231-fi8pskod 67 41 and and CC cord-104231-fi8pskod 67 42 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 67 43 domain domain NN cord-104231-fi8pskod 67 44 sequences sequence NNS cord-104231-fi8pskod 67 45 of of IN cord-104231-fi8pskod 67 46 TGN38 tgn38 CD cord-104231-fi8pskod 67 47 , , , cord-104231-fi8pskod 67 48 and and CC cord-104231-fi8pskod 67 49 ( ( -LRB- cord-104231-fi8pskod 67 50 3 3 LS cord-104231-fi8pskod 67 51 ) ) -RRB- cord-104231-fi8pskod 67 52 the the DT cord-104231-fi8pskod 67 53 pCMUIV pcmuiv NN cord-104231-fi8pskod 67 54 vector vector NN cord-104231-fi8pskod 67 55 as as IN cord-104231-fi8pskod 67 56 a a DT cord-104231-fi8pskod 67 57 HindIlI HindIlI NNP cord-104231-fi8pskod 67 58 - - HYPH cord-104231-fi8pskod 67 59 BamHI bamhi NN cord-104231-fi8pskod 67 60 DNA dna NN cord-104231-fi8pskod 67 61 fragment fragment NN cord-104231-fi8pskod 67 62 . . . cord-104231-fi8pskod 68 1 To to TO cord-104231-fi8pskod 68 2 construct construct VB cord-104231-fi8pskod 68 3 the the DT cord-104231-fi8pskod 68 4 CDS CDS NNP cord-104231-fi8pskod 68 5 - - HYPH cord-104231-fi8pskod 68 6 M M NNP cord-104231-fi8pskod 68 7 membrane membrane NN cord-104231-fi8pskod 68 8 - - HYPH cord-104231-fi8pskod 68 9 spanning span VBG cord-104231-fi8pskod 68 10 chimeric chimeric JJ cord-104231-fi8pskod 68 11 eDNA edna NN cord-104231-fi8pskod 68 12 the the DT cord-104231-fi8pskod 68 13 ligation ligation NN cord-104231-fi8pskod 68 14 contained contain VBN cord-104231-fi8pskod 68 15 ( ( -LRB- cord-104231-fi8pskod 68 16 1 1 CD cord-104231-fi8pskod 68 17 ) ) -RRB- cord-104231-fi8pskod 68 18 the the DT cord-104231-fi8pskod 68 19 HindllI HindllI NNP cord-104231-fi8pskod 68 20 - - HYPH cord-104231-fi8pskod 68 21 ApaLI apali NN cord-104231-fi8pskod 68 22 DNA dna NN cord-104231-fi8pskod 68 23 fragment fragment NN cord-104231-fi8pskod 68 24 that that WDT cord-104231-fi8pskod 68 25 encoded encode VBD cord-104231-fi8pskod 68 26 most most JJS cord-104231-fi8pskod 68 27 of of IN cord-104231-fi8pskod 68 28 the the DT cord-104231-fi8pskod 68 29 lumenal lumenal JJ cord-104231-fi8pskod 68 30 domain domain NN cord-104231-fi8pskod 68 31 of of IN cord-104231-fi8pskod 68 32 the the DT cord-104231-fi8pskod 68 33 CD8 CD8 NNP cord-104231-fi8pskod 68 34 molecule molecule NN cord-104231-fi8pskod 68 35 , , , cord-104231-fi8pskod 68 36 ( ( -LRB- cord-104231-fi8pskod 68 37 2 2 LS cord-104231-fi8pskod 68 38 ) ) -RRB- cord-104231-fi8pskod 68 39 an an DT cord-104231-fi8pskod 68 40 ApaLI ApaLI NNP cord-104231-fi8pskod 68 41 - - HYPH cord-104231-fi8pskod 68 42 SalI SalI NNP cord-104231-fi8pskod 68 43 DNA dna NN cord-104231-fi8pskod 68 44 fragment fragment NN cord-104231-fi8pskod 68 45 that that WDT cord-104231-fi8pskod 68 46 contained contain VBD cord-104231-fi8pskod 68 47 the the DT cord-104231-fi8pskod 68 48 membrane membrane NN cord-104231-fi8pskod 68 49 - - HYPH cord-104231-fi8pskod 68 50 spanning span VBG cord-104231-fi8pskod 68 51 domain domain NN cord-104231-fi8pskod 68 52 of of IN cord-104231-fi8pskod 68 53 TGN38 TGN38 NNP cord-104231-fi8pskod 68 54 , , , cord-104231-fi8pskod 68 55 and and CC cord-104231-fi8pskod 68 56 ( ( -LRB- cord-104231-fi8pskod 68 57 3 3 LS cord-104231-fi8pskod 68 58 ) ) -RRB- cord-104231-fi8pskod 68 59 the the DT cord-104231-fi8pskod 68 60 pCMUIV pcmuiv NN cord-104231-fi8pskod 68 61 plasmid plasmid NN cord-104231-fi8pskod 68 62 vector vector NN cord-104231-fi8pskod 68 63 as as IN cord-104231-fi8pskod 68 64 a a DT cord-104231-fi8pskod 68 65 HindIII HindIII NNP cord-104231-fi8pskod 68 66 - - HYPH cord-104231-fi8pskod 68 67 SalI SalI NNP cord-104231-fi8pskod 68 68 DNA dna NN cord-104231-fi8pskod 68 69 fragment fragment NN cord-104231-fi8pskod 68 70 which which WDT cord-104231-fi8pskod 68 71 also also RB cord-104231-fi8pskod 68 72 contalned contalne VBD cord-104231-fi8pskod 68 73 the the DT cord-104231-fi8pskod 68 74 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 68 75 domain domain NN cord-104231-fi8pskod 68 76 of of IN cord-104231-fi8pskod 68 77 the the DT cord-104231-fi8pskod 68 78 CD8 CD8 NNP cord-104231-fi8pskod 68 79 molecule molecule NN cord-104231-fi8pskod 68 80 . . . cord-104231-fi8pskod 69 1 To to TO cord-104231-fi8pskod 69 2 construct construct VB cord-104231-fi8pskod 69 3 the the DT cord-104231-fi8pskod 69 4 CD8-C CD8-C NNP cord-104231-fi8pskod 69 5 and and CC cord-104231-fi8pskod 69 6 CD8-AC cd8-ac NN cord-104231-fi8pskod 69 7 chimeric chimeric JJ cord-104231-fi8pskod 69 8 cDNAs cdna VBZ cord-104231-fi8pskod 69 9 the the DT cord-104231-fi8pskod 69 10 ligation ligation NN cord-104231-fi8pskod 69 11 contained contain VBN cord-104231-fi8pskod 69 12 ( ( -LRB- cord-104231-fi8pskod 69 13 1 1 CD cord-104231-fi8pskod 69 14 ) ) -RRB- cord-104231-fi8pskod 69 15 a a DT cord-104231-fi8pskod 69 16 SalI SalI NNP cord-104231-fi8pskod 69 17 - - HYPH cord-104231-fi8pskod 69 18 BamHI bamhi NN cord-104231-fi8pskod 69 19 DNA dna NN cord-104231-fi8pskod 69 20 fragment fragment NN cord-104231-fi8pskod 69 21 that that WDT cord-104231-fi8pskod 69 22 contained contain VBD cord-104231-fi8pskod 69 23 the the DT cord-104231-fi8pskod 69 24 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 69 25 domain domain NN cord-104231-fi8pskod 69 26 of of IN cord-104231-fi8pskod 69 27 TGN38 TGN38 NNP cord-104231-fi8pskod 69 28 , , , cord-104231-fi8pskod 69 29 and and CC cord-104231-fi8pskod 69 30 ( ( -LRB- cord-104231-fi8pskod 69 31 2 2 LS cord-104231-fi8pskod 69 32 ) ) -RRB- cord-104231-fi8pskod 69 33 the the DT cord-104231-fi8pskod 69 34 pCMUIV pcmuiv NN cord-104231-fi8pskod 69 35 vector vector NN cord-104231-fi8pskod 69 36 as as IN cord-104231-fi8pskod 69 37 a a DT cord-104231-fi8pskod 69 38 BamI BamI NNP cord-104231-fi8pskod 69 39 - - HYPH cord-104231-fi8pskod 69 40 II II NNP cord-104231-fi8pskod 69 41 - - HYPH cord-104231-fi8pskod 69 42 SalI SalI NNP cord-104231-fi8pskod 69 43 DNA dna NN cord-104231-fi8pskod 69 44 fragment fragment NN cord-104231-fi8pskod 69 45 which which WDT cord-104231-fi8pskod 69 46 also also RB cord-104231-fi8pskod 69 47 contained contain VBD cord-104231-fi8pskod 69 48 the the DT cord-104231-fi8pskod 69 49 lumenal lumenal JJ cord-104231-fi8pskod 69 50 and and CC cord-104231-fi8pskod 69 51 membrane membrane NN cord-104231-fi8pskod 69 52 - - HYPH cord-104231-fi8pskod 69 53 spanning span VBG cord-104231-fi8pskod 69 54 domains domain NNS cord-104231-fi8pskod 69 55 of of IN cord-104231-fi8pskod 69 56 the the DT cord-104231-fi8pskod 69 57 CD8 CD8 NNP cord-104231-fi8pskod 69 58 molecule molecule NN cord-104231-fi8pskod 69 59 . . . cord-104231-fi8pskod 70 1 Cloning clone VBG cord-104231-fi8pskod 70 2 of of IN cord-104231-fi8pskod 70 3 the the DT cord-104231-fi8pskod 70 4 CD4 CD4 NNP cord-104231-fi8pskod 70 5 eDNA edna NN cord-104231-fi8pskod 71 1 into into IN cord-104231-fi8pskod 71 2 the the DT cord-104231-fi8pskod 71 3 pCMUIV pcmuiv NN cord-104231-fi8pskod 71 4 plasmid plasmid NN cord-104231-fi8pskod 71 5 has have VBZ cord-104231-fi8pskod 71 6 been be VBN cord-104231-fi8pskod 71 7 described describe VBN cord-104231-fi8pskod 71 8 by by IN cord-104231-fi8pskod 71 9 Nilsson Nilsson NNP cord-104231-fi8pskod 71 10 et et NNP cord-104231-fi8pskod 71 11 al al NNP cord-104231-fi8pskod 71 12 . . . cord-104231-fi8pskod 72 1 ( ( -LRB- cord-104231-fi8pskod 72 2 1989 1989 CD cord-104231-fi8pskod 72 3 ) ) -RRB- cord-104231-fi8pskod 73 1 and and CC cord-104231-fi8pskod 73 2 we -PRON- PRP cord-104231-fi8pskod 73 3 used use VBD cord-104231-fi8pskod 73 4 this this DT cord-104231-fi8pskod 73 5 CD4 CD4 NNP cord-104231-fi8pskod 73 6 plasmid plasmid NN cord-104231-fi8pskod 73 7 initially initially RB cord-104231-fi8pskod 73 8 . . . cord-104231-fi8pskod 74 1 However however RB cord-104231-fi8pskod 74 2 , , , cord-104231-fi8pskod 74 3 there there EX cord-104231-fi8pskod 74 4 were be VBD cord-104231-fi8pskod 74 5 no no DT cord-104231-fi8pskod 74 6 unique unique JJ cord-104231-fi8pskod 74 7 restriction restriction NN cord-104231-fi8pskod 74 8 sites site NNS cord-104231-fi8pskod 74 9 in in IN cord-104231-fi8pskod 74 10 the the DT cord-104231-fi8pskod 74 11 CD4 CD4 NNP cord-104231-fi8pskod 74 12 eDNA edna NN cord-104231-fi8pskod 75 1 at at IN cord-104231-fi8pskod 75 2 equivalent equivalent JJ cord-104231-fi8pskod 75 3 positions position NNS cord-104231-fi8pskod 75 4 which which WDT cord-104231-fi8pskod 75 5 would would MD cord-104231-fi8pskod 75 6 have have VB cord-104231-fi8pskod 75 7 allowed allow VBN cord-104231-fi8pskod 75 8 us -PRON- PRP cord-104231-fi8pskod 75 9 to to TO cord-104231-fi8pskod 75 10 insert insert VB cord-104231-fi8pskod 75 11 replacement replacement NN cord-104231-fi8pskod 75 12 membranespanning membranespanning NN cord-104231-fi8pskod 75 13 and and CC cord-104231-fi8pskod 75 14 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 75 15 TGN38 tgn38 CD cord-104231-fi8pskod 75 16 sequences sequence NNS cord-104231-fi8pskod 75 17 . . . cord-104231-fi8pskod 76 1 We -PRON- PRP cord-104231-fi8pskod 76 2 thus thus RB cord-104231-fi8pskod 76 3 inserted insert VBD cord-104231-fi8pskod 76 4 unique unique JJ cord-104231-fi8pskod 76 5 restriction restriction NN cord-104231-fi8pskod 76 6 sites site NNS cord-104231-fi8pskod 76 7 within within IN cord-104231-fi8pskod 76 8 the the DT cord-104231-fi8pskod 76 9 CD4 CD4 NNP cord-104231-fi8pskod 76 10 eDNA edna IN cord-104231-fi8pskod 76 11 using use VBG cord-104231-fi8pskod 76 12 PCR PCR NNP cord-104231-fi8pskod 76 13 mutngenesis mutngenesis NN cord-104231-fi8pskod 76 14 . . . cord-104231-fi8pskod 77 1 Deen Deen NNP cord-104231-fi8pskod 77 2 et et FW cord-104231-fi8pskod 77 3 al al NNP cord-104231-fi8pskod 77 4 . . . cord-104231-fi8pskod 78 1 ( ( -LRB- cord-104231-fi8pskod 78 2 1988 1988 CD cord-104231-fi8pskod 78 3 ) ) -RRB- cord-104231-fi8pskod 78 4 have have VBP cord-104231-fi8pskod 78 5 shown show VBN cord-104231-fi8pskod 78 6 that that IN cord-104231-fi8pskod 78 7 a a DT cord-104231-fi8pskod 78 8 CD4 CD4 NNP cord-104231-fi8pskod 78 9 molecule molecule NN cord-104231-fi8pskod 78 10 terminating terminate VBG cord-104231-fi8pskod 78 11 after after IN cord-104231-fi8pskod 78 12 residue residue NN cord-104231-fi8pskod 78 13 371 371 CD cord-104231-fi8pskod 78 14 in in IN cord-104231-fi8pskod 78 15 the the DT cord-104231-fi8pskod 78 16 extracellular extracellular JJ cord-104231-fi8pskod 78 17 domain domain NN cord-104231-fi8pskod 78 18 is be VBZ cord-104231-fi8pskod 78 19 efficiently efficiently RB cord-104231-fi8pskod 78 20 secreted secrete VBN cord-104231-fi8pskod 78 21 . . . cord-104231-fi8pskod 79 1 As as IN cord-104231-fi8pskod 79 2 the the DT cord-104231-fi8pskod 79 3 threonine threonine NN cord-104231-fi8pskod 79 4 at at IN cord-104231-fi8pskod 79 5 369 369 CD cord-104231-fi8pskod 79 6 is be VBZ cord-104231-fi8pskod 79 7 followed follow VBN cord-104231-fi8pskod 79 8 by by IN cord-104231-fi8pskod 79 9 a a DT cord-104231-fi8pskod 79 10 proline proline NN cord-104231-fi8pskod 79 11 residue residue NN cord-104231-fi8pskod 79 12 , , , cord-104231-fi8pskod 79 13 this this DT cord-104231-fi8pskod 79 14 would would MD cord-104231-fi8pskod 79 15 make make VB cord-104231-fi8pskod 79 16 it -PRON- PRP cord-104231-fi8pskod 79 17 unlikely unlikely JJ cord-104231-fi8pskod 79 18 that that IN cord-104231-fi8pskod 79 19 there there EX cord-104231-fi8pskod 79 20 are be VBP cord-104231-fi8pskod 79 21 any any DT cord-104231-fi8pskod 79 22 structural structural JJ cord-104231-fi8pskod 79 23 features feature NNS cord-104231-fi8pskod 79 24 after after IN cord-104231-fi8pskod 79 25 thr-369 thr-369 NN cord-104231-fi8pskod 79 26 necessary necessary JJ cord-104231-fi8pskod 79 27 for for IN cord-104231-fi8pskod 79 28 correct correct JJ cord-104231-fi8pskod 79 29 folding folding NN cord-104231-fi8pskod 79 30 and and CC cord-104231-fi8pskod 79 31 transport transport NN cord-104231-fi8pskod 79 32 of of IN cord-104231-fi8pskod 79 33 CD4 CD4 NNP cord-104231-fi8pskod 79 34 . . . cord-104231-fi8pskod 80 1 Codon Codon NNP cord-104231-fi8pskod 80 2 367 367 CD cord-104231-fi8pskod 81 1 ( ( -LRB- cord-104231-fi8pskod 81 2 Maddon Maddon NNP cord-104231-fi8pskod 81 3 et et NNP cord-104231-fi8pskod 81 4 al al NNP cord-104231-fi8pskod 81 5 . . NNP cord-104231-fi8pskod 81 6 , , , cord-104231-fi8pskod 81 7 1985 1985 CD cord-104231-fi8pskod 81 8 ) ) -RRB- cord-104231-fi8pskod 81 9 in in IN cord-104231-fi8pskod 81 10 the the DT cord-104231-fi8pskod 81 11 CD4 CD4 NNP cord-104231-fi8pskod 81 12 eDNA edna NN cord-104231-fi8pskod 82 1 ( ( -LRB- cord-104231-fi8pskod 82 2 which which WDT cord-104231-fi8pskod 82 3 encodes encode VBZ cord-104231-fi8pskod 82 4 a a DT cord-104231-fi8pskod 82 5 serine serine NN cord-104231-fi8pskod 82 6 residue residue NN cord-104231-fi8pskod 82 7 ) ) -RRB- cord-104231-fi8pskod 82 8 was be VBD cord-104231-fi8pskod 82 9 mutated mutate VBN cord-104231-fi8pskod 82 10 from from IN cord-104231-fi8pskod 82 11 TCC TCC NNP cord-104231-fi8pskod 82 12 to to IN cord-104231-fi8pskod 82 13 TCA TCA NNP cord-104231-fi8pskod 82 14 . . . cord-104231-fi8pskod 83 1 This this DT cord-104231-fi8pskod 83 2 silent silent JJ cord-104231-fi8pskod 83 3 mutation mutation NN cord-104231-fi8pskod 83 4 created create VBD cord-104231-fi8pskod 83 5 a a DT cord-104231-fi8pskod 83 6 single single JJ cord-104231-fi8pskod 83 7 HincII HincII NNP cord-104231-fi8pskod 83 8 site site NN cord-104231-fi8pskod 83 9 within within IN cord-104231-fi8pskod 83 10 the the DT cord-104231-fi8pskod 83 11 CD4 CD4 NNP cord-104231-fi8pskod 83 12 eDNA edna NN cord-104231-fi8pskod 83 13 . . . cord-104231-fi8pskod 84 1 Similarly similarly RB cord-104231-fi8pskod 84 2 , , , cord-104231-fi8pskod 84 3 codons codon NNS cord-104231-fi8pskod 84 4 400 400 CD cord-104231-fi8pskod 84 5 and and CC cord-104231-fi8pskod 84 6 401 401 CD cord-104231-fi8pskod 84 7 ( ( -LRB- cord-104231-fi8pskod 84 8 encoding encode VBG cord-104231-fi8pskod 84 9 arginine arginine NN cord-104231-fi8pskod 84 10 residues residue NNS cord-104231-fi8pskod 84 11 ) ) -RRB- cord-104231-fi8pskod 84 12 were be VBD cord-104231-fi8pskod 84 13 mutated mutate VBN cord-104231-fi8pskod 84 14 from from IN cord-104231-fi8pskod 84 15 CGA CGA NNP cord-104231-fi8pskod 84 16 AGG AGG NNP cord-104231-fi8pskod 84 17 to to IN cord-104231-fi8pskod 84 18 CGT CGT NNP cord-104231-fi8pskod 84 19 CGA CGA NNP cord-104231-fi8pskod 84 20 . . . cord-104231-fi8pskod 85 1 These these DT cord-104231-fi8pskod 85 2 silent silent JJ cord-104231-fi8pskod 85 3 mutations mutation NNS cord-104231-fi8pskod 85 4 created create VBD cord-104231-fi8pskod 85 5 a a DT cord-104231-fi8pskod 85 6 unique unique JJ cord-104231-fi8pskod 85 7 SalI SalI NNP cord-104231-fi8pskod 85 8 site site NN cord-104231-fi8pskod 85 9 in in IN cord-104231-fi8pskod 85 10 the the DT cord-104231-fi8pskod 85 11 CD4 CD4 NNP cord-104231-fi8pskod 85 12 eDNA edna NN cord-104231-fi8pskod 85 13 . . . cord-104231-fi8pskod 86 1 There there EX cord-104231-fi8pskod 86 2 were be VBD cord-104231-fi8pskod 86 3 now now RB cord-104231-fi8pskod 86 4 unique unique JJ cord-104231-fi8pskod 86 5 restriction restriction NN cord-104231-fi8pskod 86 6 sites site NNS cord-104231-fi8pskod 86 7 on on IN cord-104231-fi8pskod 86 8 either either DT cord-104231-fi8pskod 86 9 side side NN cord-104231-fi8pskod 86 10 of of IN cord-104231-fi8pskod 86 11 the the DT cord-104231-fi8pskod 86 12 membranespanning membranespanne VBG cord-104231-fi8pskod 86 13 region region NN cord-104231-fi8pskod 86 14 in in IN cord-104231-fi8pskod 87 1 the the DT cord-104231-fi8pskod 87 2 eDNA edna NN cord-104231-fi8pskod 87 3 . . . cord-104231-fi8pskod 87 4 PCR PCR NNP cord-104231-fi8pskod 87 5 was be VBD cord-104231-fi8pskod 87 6 carried carry VBN cord-104231-fi8pskod 87 7 out out RP cord-104231-fi8pskod 87 8 on on IN cord-104231-fi8pskod 87 9 the the DT cord-104231-fi8pskod 87 10 TGN38 TGN38 NNP cord-104231-fi8pskod 87 11 eDNA edna IN cord-104231-fi8pskod 87 12 using use VBG cord-104231-fi8pskod 87 13 ollgonucleotides ollgonucleotide NNS cord-104231-fi8pskod 87 14 similar similar JJ cord-104231-fi8pskod 87 15 to to IN cord-104231-fi8pskod 87 16 those those DT cord-104231-fi8pskod 87 17 described describe VBN cord-104231-fi8pskod 87 18 above above RB cord-104231-fi8pskod 87 19 . . . cord-104231-fi8pskod 88 1 Instead instead RB cord-104231-fi8pskod 88 2 of of IN cord-104231-fi8pskod 88 3 the the DT cord-104231-fi8pskod 88 4 ApaLI ApaLI NNP cord-104231-fi8pskod 88 5 site site NN cord-104231-fi8pskod 88 6 used use VBN cord-104231-fi8pskod 88 7 in in IN cord-104231-fi8pskod 88 8 CD8 CD8 NNP cord-104231-fi8pskod 88 9 chimera chimera NN cord-104231-fi8pskod 88 10 constructions construction NNS cord-104231-fi8pskod 88 11 , , , cord-104231-fi8pskod 88 12 5 5 CD cord-104231-fi8pskod 88 13 ' ' POS cord-104231-fi8pskod 88 14 oligonucleotides oligonucleotide NNS cord-104231-fi8pskod 88 15 that that WDT cord-104231-fi8pskod 88 16 precede precede VBP cord-104231-fi8pskod 88 17 the the DT cord-104231-fi8pskod 88 18 TGN38 TGN38 NNP cord-104231-fi8pskod 88 19 membrane membrane NN cord-104231-fi8pskod 88 20 - - HYPH cord-104231-fi8pskod 88 21 spanning span VBG cord-104231-fi8pskod 88 22 domain domain NN cord-104231-fi8pskod 88 23 were be VBD cord-104231-fi8pskod 88 24 synthesized synthesize VBN cord-104231-fi8pskod 88 25 with with IN cord-104231-fi8pskod 88 26 a a DT cord-104231-fi8pskod 88 27 HincH HincH NNP cord-104231-fi8pskod 88 28 site site NN cord-104231-fi8pskod 88 29 . . . cord-104231-fi8pskod 89 1 A a DT cord-104231-fi8pskod 89 2 HincH HincH NNP cord-104231-fi8pskod 89 3 - - HYPH cord-104231-fi8pskod 89 4 SalI SalI NNP cord-104231-fi8pskod 89 5 or or CC cord-104231-fi8pskod 89 6 HincH HincH NNP cord-104231-fi8pskod 89 7 - - HYPH cord-104231-fi8pskod 89 8 BamHI bamhi NN cord-104231-fi8pskod 89 9 DNA dna NN cord-104231-fi8pskod 89 10 fragment fragment NN cord-104231-fi8pskod 89 11 containing contain VBG cord-104231-fi8pskod 89 12 TGN38 TGN38 NNP cord-104231-fi8pskod 89 13 sequences sequence NNS cord-104231-fi8pskod 89 14 could could MD cord-104231-fi8pskod 89 15 be be VB cord-104231-fi8pskod 89 16 fused fuse VBN cord-104231-fi8pskod 89 17 to to IN cord-104231-fi8pskod 89 18 CD4 CD4 NNP cord-104231-fi8pskod 89 19 by by IN cord-104231-fi8pskod 89 20 ligation ligation NN cord-104231-fi8pskod 89 21 as as IN cord-104231-fi8pskod 89 22 described describe VBN cord-104231-fi8pskod 89 23 previously previously RB cord-104231-fi8pskod 89 24 . . . cord-104231-fi8pskod 90 1 We -PRON- PRP cord-104231-fi8pskod 90 2 thus thus RB cord-104231-fi8pskod 90 3 ligated ligate VBD cord-104231-fi8pskod 90 4 either either CC cord-104231-fi8pskod 90 5 a a DT cord-104231-fi8pskod 90 6 HindlII HindlII NNP cord-104231-fi8pskod 90 7 - - HYPH cord-104231-fi8pskod 90 8 HincII HincII NNP cord-104231-fi8pskod 90 9 or or CC cord-104231-fi8pskod 90 10 HindIII HindIII NNP cord-104231-fi8pskod 90 11 - - HYPH cord-104231-fi8pskod 90 12 SalI SalI NNP cord-104231-fi8pskod 90 13 DNA dna NN cord-104231-fi8pskod 90 14 fragment fragment NN cord-104231-fi8pskod 90 15 ( ( -LRB- cord-104231-fi8pskod 90 16 encoding encode VBG cord-104231-fi8pskod 90 17 the the DT cord-104231-fi8pskod 90 18 lumenal lumenal JJ cord-104231-fi8pskod 90 19 domain domain NN cord-104231-fi8pskod 90 20 of of IN cord-104231-fi8pskod 90 21 the the DT cord-104231-fi8pskod 90 22 CD4 CD4 NNP cord-104231-fi8pskod 90 23 molecule molecule NN cord-104231-fi8pskod 90 24 ) ) -RRB- cord-104231-fi8pskod 90 25 from from IN cord-104231-fi8pskod 90 26 pCMUIV pcmuiv NN cord-104231-fi8pskod 90 27 - - HYPH cord-104231-fi8pskod 90 28 CD4 CD4 NNP cord-104231-fi8pskod 90 29 , , , cord-104231-fi8pskod 90 30 an an DT cord-104231-fi8pskod 90 31 appropriate appropriate JJ cord-104231-fi8pskod 90 32 PCR PCR NNP cord-104231-fi8pskod 90 33 generated generate VBN cord-104231-fi8pskod 90 34 DNA dna NN cord-104231-fi8pskod 90 35 fragment fragment NN cord-104231-fi8pskod 90 36 that that WDT cord-104231-fi8pskod 90 37 encoded encode VBD cord-104231-fi8pskod 90 38 TGN38 TGN38 NNP cord-104231-fi8pskod 90 39 sequences sequence NNS cord-104231-fi8pskod 90 40 , , , cord-104231-fi8pskod 90 41 and and CC cord-104231-fi8pskod 90 42 an an DT cord-104231-fi8pskod 90 43 appropriate appropriate JJ cord-104231-fi8pskod 90 44 pCMUIV pcmuiv NN cord-104231-fi8pskod 90 45 vector vector NN cord-104231-fi8pskod 90 46 . . . cord-104231-fi8pskod 91 1 Ligations ligation NNS cord-104231-fi8pskod 91 2 were be VBD cord-104231-fi8pskod 91 3 transformed transform VBN cord-104231-fi8pskod 91 4 into into IN cord-104231-fi8pskod 91 5 E. E. NNP cord-104231-fi8pskod 91 6 coil coil NN cord-104231-fi8pskod 91 7 strain strain NN cord-104231-fi8pskod 91 8 JM101 jm101 IN cord-104231-fi8pskod 91 9 using use VBG cord-104231-fi8pskod 91 10 standard standard JJ cord-104231-fi8pskod 91 11 procedures procedure NNS cord-104231-fi8pskod 91 12 ( ( -LRB- cord-104231-fi8pskod 91 13 Sambrook Sambrook NNP cord-104231-fi8pskod 91 14 et et NNP cord-104231-fi8pskod 91 15 al al NNP cord-104231-fi8pskod 91 16 . . NNP cord-104231-fi8pskod 91 17 , , , cord-104231-fi8pskod 91 18 1989 1989 CD cord-104231-fi8pskod 91 19 ) ) -RRB- cord-104231-fi8pskod 91 20 and and CC cord-104231-fi8pskod 91 21 clones clone NNS cord-104231-fi8pskod 91 22 were be VBD cord-104231-fi8pskod 91 23 analyzed analyze VBN cord-104231-fi8pskod 91 24 by by IN cord-104231-fi8pskod 91 25 restriction restriction NN cord-104231-fi8pskod 91 26 digestion digestion NN cord-104231-fi8pskod 91 27 followed follow VBN cord-104231-fi8pskod 91 28 by by IN cord-104231-fi8pskod 91 29 DNA dna NN cord-104231-fi8pskod 91 30 sequencing sequencing NN cord-104231-fi8pskod 91 31 . . . cord-104231-fi8pskod 92 1 Large large JJ cord-104231-fi8pskod 92 2 - - HYPH cord-104231-fi8pskod 92 3 scale scale NN cord-104231-fi8pskod 92 4 preparations preparation NNS cord-104231-fi8pskod 92 5 of of IN cord-104231-fi8pskod 92 6 the the DT cord-104231-fi8pskod 92 7 pCMUIV pcmuiv NN cord-104231-fi8pskod 92 8 plasmids plasmid NNS cord-104231-fi8pskod 92 9 ( ( -LRB- cord-104231-fi8pskod 92 10 for for IN cord-104231-fi8pskod 92 11 transfections transfection NNS cord-104231-fi8pskod 92 12 ) ) -RRB- cord-104231-fi8pskod 92 13 were be VBD cord-104231-fi8pskod 92 14 made make VBN cord-104231-fi8pskod 92 15 by by IN cord-104231-fi8pskod 92 16 alkaline alkaline JJ cord-104231-fi8pskod 92 17 lysis lysis NN cord-104231-fi8pskod 92 18 followed follow VBN cord-104231-fi8pskod 92 19 by by IN cord-104231-fi8pskod 92 20 CsCl CsCl NNP cord-104231-fi8pskod 92 21 - - HYPH cord-104231-fi8pskod 92 22 ethidium ethidium NN cord-104231-fi8pskod 92 23 bromide bromide NN cord-104231-fi8pskod 92 24 purification purification NN cord-104231-fi8pskod 92 25 ( ( -LRB- cord-104231-fi8pskod 92 26 Sambrook Sambrook NNP cord-104231-fi8pskod 92 27 et et NNP cord-104231-fi8pskod 92 28 al al NNP cord-104231-fi8pskod 92 29 . . NNP cord-104231-fi8pskod 92 30 , , , cord-104231-fi8pskod 92 31 1989 1989 CD cord-104231-fi8pskod 92 32 ) ) -RRB- cord-104231-fi8pskod 92 33 . . . cord-104231-fi8pskod 93 1 Monolayer Monolayer NNP cord-104231-fi8pskod 93 2 HeLa HeLa NNP cord-104231-fi8pskod 93 3 cells cell NNS cord-104231-fi8pskod 93 4 ( ( -LRB- cord-104231-fi8pskod 93 5 ATCC ATCC NNP cord-104231-fi8pskod 93 6 CCL185 CCL185 NNP cord-104231-fi8pskod 93 7 ) ) -RRB- cord-104231-fi8pskod 93 8 were be VBD cord-104231-fi8pskod 93 9 grown grow VBN cord-104231-fi8pskod 93 10 in in IN cord-104231-fi8pskod 93 11 DMEM DMEM NNP cord-104231-fi8pskod 93 12 supplemented supplement VBD cord-104231-fi8pskod 93 13 with with IN cord-104231-fi8pskod 93 14 10 10 CD cord-104231-fi8pskod 93 15 % % NN cord-104231-fi8pskod 93 16 FCS FCS NNP cord-104231-fi8pskod 93 17 , , , cord-104231-fi8pskod 93 18 non non JJ cord-104231-fi8pskod 93 19 - - JJ cord-104231-fi8pskod 93 20 essential essential JJ cord-104231-fi8pskod 93 21 amino amino JJ cord-104231-fi8pskod 93 22 acids acid NNS cord-104231-fi8pskod 93 23 , , , cord-104231-fi8pskod 93 24 penicillin penicillin NN cord-104231-fi8pskod 93 25 / / SYM cord-104231-fi8pskod 93 26 streptomycin streptomycin NNS cord-104231-fi8pskod 93 27 , , , cord-104231-fi8pskod 93 28 and and CC cord-104231-fi8pskod 93 29 2 2 CD cord-104231-fi8pskod 93 30 mM mm CD cord-104231-fi8pskod 93 31 glutamine glutamine NN cord-104231-fi8pskod 93 32 . . . cord-104231-fi8pskod 94 1 Cells cell NNS cord-104231-fi8pskod 94 2 were be VBD cord-104231-fi8pskod 94 3 grown grow VBN cord-104231-fi8pskod 94 4 to to IN cord-104231-fi8pskod 94 5 20 20 CD cord-104231-fi8pskod 94 6 - - SYM cord-104231-fi8pskod 94 7 30 30 CD cord-104231-fi8pskod 94 8 % % NN cord-104231-fi8pskod 94 9 conttuency conttuency NN cord-104231-fi8pskod 94 10 before before IN cord-104231-fi8pskod 94 11 transfection transfection NN cord-104231-fi8pskod 94 12 by by IN cord-104231-fi8pskod 94 13 the the DT cord-104231-fi8pskod 94 14 calcium calcium NN cord-104231-fi8pskod 94 15 phosphate phosphate NN cord-104231-fi8pskod 94 16 method method NN cord-104231-fi8pskod 94 17 essentially essentially RB cord-104231-fi8pskod 94 18 as as IN cord-104231-fi8pskod 94 19 described describe VBN cord-104231-fi8pskod 94 20 by by IN cord-104231-fi8pskod 94 21 Nilsson Nilsson NNP cord-104231-fi8pskod 94 22 et et NNP cord-104231-fi8pskod 94 23 al al NNP cord-104231-fi8pskod 94 24 . . . cord-104231-fi8pskod 95 1 ( ( -LRB- cord-104231-fi8pskod 95 2 1989 1989 CD cord-104231-fi8pskod 95 3 ) ) -RRB- cord-104231-fi8pskod 95 4 . . . cord-104231-fi8pskod 96 1 For for IN cord-104231-fi8pskod 96 2 immunottuorescence immunottuorescence NN cord-104231-fi8pskod 96 3 , , , cord-104231-fi8pskod 96 4 cells cell NNS cord-104231-fi8pskod 96 5 were be VBD cord-104231-fi8pskod 96 6 seeded seed VBN cord-104231-fi8pskod 96 7 out out RP cord-104231-fi8pskod 96 8 into into IN cord-104231-fi8pskod 96 9 30-ram 30-ram CD cord-104231-fi8pskod 96 10 , , , cord-104231-fi8pskod 96 11 6-weil 6-weil CD cord-104231-fi8pskod 96 12 plates plate NNS cord-104231-fi8pskod 96 13 ( ( -LRB- cord-104231-fi8pskod 96 14 Becton Becton NNP cord-104231-fi8pskod 96 15 Dickinson Dickinson NNP cord-104231-fi8pskod 96 16 , , , cord-104231-fi8pskod 96 17 Lincoln Lincoln NNP cord-104231-fi8pskod 96 18 Park Park NNP cord-104231-fi8pskod 96 19 , , , cord-104231-fi8pskod 96 20 NJ NJ NNP cord-104231-fi8pskod 96 21 ) ) -RRB- cord-104231-fi8pskod 96 22 containing contain VBG cord-104231-fi8pskod 96 23 sterile sterile JJ cord-104231-fi8pskod 96 24 coverslips coverslip NNS cord-104231-fi8pskod 96 25 . . . cord-104231-fi8pskod 97 1 After after IN cord-104231-fi8pskod 97 2 one one CD cord-104231-fi8pskod 97 3 day day NN cord-104231-fi8pskod 97 4 , , , cord-104231-fi8pskod 97 5 cells cell NNS cord-104231-fi8pskod 97 6 were be VBD cord-104231-fi8pskod 97 7 transfected transfecte VBN cord-104231-fi8pskod 97 8 with with IN cord-104231-fi8pskod 97 9 2 2 CD cord-104231-fi8pskod 97 10 ~g ~g NN cord-104231-fi8pskod 97 11 of of IN cord-104231-fi8pskod 97 12 plasmid plasmid NN cord-104231-fi8pskod 97 13 DNA DNA NNP cord-104231-fi8pskod 97 14 ( ( -LRB- cord-104231-fi8pskod 97 15 CsCI CsCI NNP cord-104231-fi8pskod 97 16 - - HYPH cord-104231-fi8pskod 97 17 EtBr EtBr NNP cord-104231-fi8pskod 97 18 purified purify VBN cord-104231-fi8pskod 97 19 ) ) -RRB- cord-104231-fi8pskod 97 20 and and CC cord-104231-fi8pskod 97 21 3 3 CD cord-104231-fi8pskod 97 22 / / SYM cord-104231-fi8pskod 97 23 tg tg NNP cord-104231-fi8pskod 97 24 of of IN cord-104231-fi8pskod 97 25 pUC19 pUC19 NNP cord-104231-fi8pskod 97 26 plasmid plasmid NNP cord-104231-fi8pskod 97 27 DNA DNA NNP cord-104231-fi8pskod 97 28 ( ( -LRB- cord-104231-fi8pskod 97 29 used use VBN cord-104231-fi8pskod 97 30 as as IN cord-104231-fi8pskod 97 31 a a DT cord-104231-fi8pskod 97 32 carrier carrier NN cord-104231-fi8pskod 97 33 to to TO cord-104231-fi8pskod 97 34 increase increase VB cord-104231-fi8pskod 97 35 transfection transfection NN cord-104231-fi8pskod 97 36 efficiency efficiency NN cord-104231-fi8pskod 97 37 ) ) -RRB- cord-104231-fi8pskod 97 38 . . . cord-104231-fi8pskod 98 1 Cells cell NNS cord-104231-fi8pskod 98 2 were be VBD cord-104231-fi8pskod 98 3 fixed fix VBN cord-104231-fi8pskod 98 4 and and CC cord-104231-fi8pskod 98 5 processed process VBN cord-104231-fi8pskod 98 6 for for IN cord-104231-fi8pskod 98 7 immunottuorescence immunottuorescence NN cord-104231-fi8pskod 98 8 60 60 CD cord-104231-fi8pskod 98 9 - - SYM cord-104231-fi8pskod 98 10 72 72 CD cord-104231-fi8pskod 98 11 h h NN cord-104231-fi8pskod 98 12 later later RB cord-104231-fi8pskod 99 1 ( ( -LRB- cord-104231-fi8pskod 99 2 Nilsson Nilsson NNP cord-104231-fi8pskod 99 3 et et NNP cord-104231-fi8pskod 99 4 al al NNP cord-104231-fi8pskod 99 5 . . NNP cord-104231-fi8pskod 99 6 , , , cord-104231-fi8pskod 99 7 1989 1989 CD cord-104231-fi8pskod 99 8 ; ; : cord-104231-fi8pskod 99 9 Jackson Jackson NNP cord-104231-fi8pskod 99 10 et et NNP cord-104231-fi8pskod 99 11 al al NNP cord-104231-fi8pskod 99 12 . . NNP cord-104231-fi8pskod 99 13 , , , cord-104231-fi8pskod 99 14 1990 1990 CD cord-104231-fi8pskod 99 15 ) ) -RRB- cord-104231-fi8pskod 99 16 . . . cord-104231-fi8pskod 100 1 Transfected transfecte VBN cord-104231-fi8pskod 100 2 cells cell NNS cord-104231-fi8pskod 100 3 for for IN cord-104231-fi8pskod 100 4 electron electron NN cord-104231-fi8pskod 100 5 microscopy microscopy NNS cord-104231-fi8pskod 100 6 and and CC cord-104231-fi8pskod 100 7 immunoprecipitation immunoprecipitation NN cord-104231-fi8pskod 100 8 were be VBD cord-104231-fi8pskod 100 9 prepared prepare VBN cord-104231-fi8pskod 100 10 as as IN cord-104231-fi8pskod 100 11 described describe VBN cord-104231-fi8pskod 100 12 by by IN cord-104231-fi8pskod 100 13 Nilsson Nilsson NNP cord-104231-fi8pskod 100 14 et et NNP cord-104231-fi8pskod 100 15 al al NNP cord-104231-fi8pskod 100 16 . . . cord-104231-fi8pskod 101 1 ( ( -LRB- cord-104231-fi8pskod 101 2 1989 1989 CD cord-104231-fi8pskod 101 3 ) ) -RRB- cord-104231-fi8pskod 101 4 in in IN cord-104231-fi8pskod 101 5 100-mm 100-mm CD cord-104231-fi8pskod 101 6 tissue tissue NN cord-104231-fi8pskod 101 7 culture culture NN cord-104231-fi8pskod 101 8 dishes dish NNS cord-104231-fi8pskod 101 9 and and CC cord-104231-fi8pskod 101 10 processed process VBD cord-104231-fi8pskod 101 11 60 60 CD cord-104231-fi8pskod 101 12 - - SYM cord-104231-fi8pskod 101 13 72 72 CD cord-104231-fi8pskod 101 14 h h NN cord-104231-fi8pskod 101 15 after after IN cord-104231-fi8pskod 101 16 DNA DNA NNP cord-104231-fi8pskod 101 17 was be VBD cord-104231-fi8pskod 101 18 added add VBN cord-104231-fi8pskod 101 19 . . . cord-104231-fi8pskod 102 1 Cells cell NNS cord-104231-fi8pskod 102 2 were be VBD cord-104231-fi8pskod 102 3 processed process VBN cord-104231-fi8pskod 102 4 for for IN cord-104231-fi8pskod 102 5 immunofluorescence immunofluorescence NN cord-104231-fi8pskod 102 6 microscopy microscopy NN cord-104231-fi8pskod 102 7 as as IN cord-104231-fi8pskod 102 8 described describe VBN cord-104231-fi8pskod 102 9 previously previously RB cord-104231-fi8pskod 102 10 ( ( -LRB- cord-104231-fi8pskod 102 11 Warren Warren NNP cord-104231-fi8pskod 102 12 et et NNP cord-104231-fi8pskod 102 13 al al NNP cord-104231-fi8pskod 102 14 . . NNP cord-104231-fi8pskod 102 15 , , , cord-104231-fi8pskod 102 16 1984 1984 CD cord-104231-fi8pskod 102 17 ; ; : cord-104231-fi8pskod 102 18 Nilsson Nilsson NNP cord-104231-fi8pskod 102 19 et et NNP cord-104231-fi8pskod 102 20 al al NNP cord-104231-fi8pskod 102 21 . . NNP cord-104231-fi8pskod 102 22 , , , cord-104231-fi8pskod 102 23 1991 1991 CD cord-104231-fi8pskod 102 24 ) ) -RRB- cord-104231-fi8pskod 102 25 . . . cord-104231-fi8pskod 103 1 The the DT cord-104231-fi8pskod 103 2 rabbit rabbit NN cord-104231-fi8pskod 103 3 antiserum antiserum NN cord-104231-fi8pskod 103 4 to to IN cord-104231-fi8pskod 103 5 TGN38 TGN38 NNP cord-104231-fi8pskod 103 6 has have VBZ cord-104231-fi8pskod 103 7 been be VBN cord-104231-fi8pskod 103 8 described describe VBN cord-104231-fi8pskod 103 9 by by IN cord-104231-fi8pskod 103 10 Luzio Luzio NNP cord-104231-fi8pskod 103 11 et et NNP cord-104231-fi8pskod 103 12 al al NNP cord-104231-fi8pskod 103 13 . . . cord-104231-fi8pskod 104 1 ( ( -LRB- cord-104231-fi8pskod 104 2 1990 1990 CD cord-104231-fi8pskod 104 3 ) ) -RRB- cord-104231-fi8pskod 104 4 and and CC cord-104231-fi8pskod 104 5 was be VBD cord-104231-fi8pskod 104 6 diluted dilute VBN cord-104231-fi8pskod 104 7 ( ( -LRB- cord-104231-fi8pskod 104 8 1:500 1:500 CD cord-104231-fi8pskod 104 9 ) ) -RRB- cord-104231-fi8pskod 104 10 in in IN cord-104231-fi8pskod 104 11 0.2 0.2 CD cord-104231-fi8pskod 104 12 % % NN cord-104231-fi8pskod 104 13 fish fish NN cord-104231-fi8pskod 104 14 skin skin NN cord-104231-fi8pskod 104 15 gelatin gelatin NN cord-104231-fi8pskod 104 16 / / SYM cord-104231-fi8pskod 104 17 PBS PBS NNP cord-104231-fi8pskod 104 18 before before IN cord-104231-fi8pskod 104 19 use use NN cord-104231-fi8pskod 104 20 . . . cord-104231-fi8pskod 105 1 GaIT GaIT NNP cord-104231-fi8pskod 105 2 was be VBD cord-104231-fi8pskod 105 3 detected detect VBN cord-104231-fi8pskod 105 4 using use VBG cord-104231-fi8pskod 105 5 either either CC cord-104231-fi8pskod 105 6 a a DT cord-104231-fi8pskod 105 7 rabbit rabbit NN cord-104231-fi8pskod 105 8 polyclonal polyclonal JJ cord-104231-fi8pskod 105 9 antiserum antiserum NN cord-104231-fi8pskod 105 10 ( ( -LRB- cord-104231-fi8pskod 105 11 Roth Roth NNP cord-104231-fi8pskod 105 12 and and CC cord-104231-fi8pskod 105 13 Berger Berger NNP cord-104231-fi8pskod 105 14 , , , cord-104231-fi8pskod 105 15 1982 1982 CD cord-104231-fi8pskod 105 16 ; ; : cord-104231-fi8pskod 106 1 Nilsson Nilsson NNP cord-104231-fi8pskod 106 2 et et NNP cord-104231-fi8pskod 106 3 al al NNP cord-104231-fi8pskod 106 4 . . . cord-104231-fi8pskod 107 1 , , , cord-104231-fi8pskod 107 2 1993a 1993a CD cord-104231-fi8pskod 107 3 ) ) -RRB- cord-104231-fi8pskod 107 4 at at IN cord-104231-fi8pskod 107 5 a a DT cord-104231-fi8pskod 107 6 dilution dilution NN cord-104231-fi8pskod 107 7 of of IN cord-104231-fi8pskod 107 8 1:200 1:200 CD cord-104231-fi8pskod 107 9 or or CC cord-104231-fi8pskod 107 10 the the DT cord-104231-fi8pskod 107 11 monoclonal monoclonal JJ cord-104231-fi8pskod 107 12 antibody antibody NN cord-104231-fi8pskod 107 13 GT-2 GT-2 NNP cord-104231-fi8pskod 108 1 ( ( -LRB- cord-104231-fi8pskod 108 2 Berger Berger NNP cord-104231-fi8pskod 108 3 et et NNP cord-104231-fi8pskod 108 4 al al NNP cord-104231-fi8pskod 108 5 . . NNP cord-104231-fi8pskod 108 6 , , , cord-104231-fi8pskod 108 7 1986 1986 CD cord-104231-fi8pskod 108 8 ) ) -RRB- cord-104231-fi8pskod 108 9 . . . cord-104231-fi8pskod 109 1 Culture culture NN cord-104231-fi8pskod 109 2 supernatants supernatant NNS cord-104231-fi8pskod 109 3 from from IN cord-104231-fi8pskod 109 4 the the DT cord-104231-fi8pskod 109 5 QS4120 QS4120 NNP cord-104231-fi8pskod 110 1 ( ( -LRB- cord-104231-fi8pskod 110 2 Healey Healey NNP cord-104231-fi8pskod 110 3 et et NNP cord-104231-fi8pskod 110 4 al al NNP cord-104231-fi8pskod 110 5 . . NNP cord-104231-fi8pskod 110 6 , , , cord-104231-fi8pskod 110 7 1990 1990 CD cord-104231-fi8pskod 110 8 ) ) -RRB- cord-104231-fi8pskod 110 9 and and CC cord-104231-fi8pskod 110 10 OKT8 OKT8 NNP cord-104231-fi8pskod 111 1 ( ( -LRB- cord-104231-fi8pskod 111 2 ATCC ATCC NNP cord-104231-fi8pskod 111 3 CRL CRL NNP cord-104231-fi8pskod 111 4 8014 8014 CD cord-104231-fi8pskod 111 5 ) ) -RRB- cord-104231-fi8pskod 111 6 mouse mouse NN cord-104231-fi8pskod 111 7 bybridomas bybridomas NNP cord-104231-fi8pskod 111 8 were be VBD cord-104231-fi8pskod 111 9 used use VBN cord-104231-fi8pskod 111 10 to to TO cord-104231-fi8pskod 111 11 detect detect VB cord-104231-fi8pskod 111 12 CD4 CD4 NNP cord-104231-fi8pskod 111 13 and and CC cord-104231-fi8pskod 111 14 CDS CDS NNP cord-104231-fi8pskod 111 15 , , , cord-104231-fi8pskod 111 16 respectively respectively RB cord-104231-fi8pskod 111 17 . . . cord-104231-fi8pskod 112 1 The the DT cord-104231-fi8pskod 112 2 secondary secondary JJ cord-104231-fi8pskod 112 3 antibodies antibody NNS cord-104231-fi8pskod 112 4 were be VBD cord-104231-fi8pskod 112 5 horse horse NN cord-104231-fi8pskod 112 6 anti anti JJ cord-104231-fi8pskod 112 7 = = NN cord-104231-fi8pskod 112 8 mouse mouse NN cord-104231-fi8pskod 112 9 conjngated conjngate VBN cord-104231-fi8pskod 112 10 to to IN cord-104231-fi8pskod 112 11 Texas Texas NNP cord-104231-fi8pskod 112 12 red red JJ cord-104231-fi8pskod 112 13 ( ( -LRB- cord-104231-fi8pskod 112 14 Vector Vector NNP cord-104231-fi8pskod 112 15 Labs Labs NNP cord-104231-fi8pskod 112 16 , , , cord-104231-fi8pskod 112 17 Burlingame Burlingame NNP cord-104231-fi8pskod 112 18 , , , cord-104231-fi8pskod 112 19 CA CA NNP cord-104231-fi8pskod 112 20 ) ) -RRB- cord-104231-fi8pskod 112 21 and and CC cord-104231-fi8pskod 112 22 sheep sheep NN cord-104231-fi8pskod 112 23 anti anti JJ cord-104231-fi8pskod 112 24 - - JJ cord-104231-fi8pskod 112 25 rabbit rabbit JJ cord-104231-fi8pskod 112 26 conjugated conjugate VBN cord-104231-fi8pskod 112 27 to to IN cord-104231-fi8pskod 112 28 FITC FITC NNP cord-104231-fi8pskod 112 29 ( ( -LRB- cord-104231-fi8pskod 112 30 Dakopatts Dakopatts NNP cord-104231-fi8pskod 112 31 , , , cord-104231-fi8pskod 112 32 Copenhagen Copenhagen NNP cord-104231-fi8pskod 112 33 ) ) -RRB- cord-104231-fi8pskod 112 34 and and CC cord-104231-fi8pskod 112 35 both both DT cord-104231-fi8pskod 112 36 were be VBD cord-104231-fi8pskod 112 37 used use VBN cord-104231-fi8pskod 112 38 at at IN cord-104231-fi8pskod 112 39 a a DT cord-104231-fi8pskod 112 40 dilution dilution NN cord-104231-fi8pskod 112 41 of of IN cord-104231-fi8pskod 112 42 1:100 1:100 CD cord-104231-fi8pskod 112 43 . . . cord-104231-fi8pskod 113 1 P-,hodamine P-,hodamine NNP cord-104231-fi8pskod 113 2 - - HYPH cord-104231-fi8pskod 113 3 conjngated conjngate VBN cord-104231-fi8pskod 113 4 transferrin transferrin NN cord-104231-fi8pskod 113 5 ( ( -LRB- cord-104231-fi8pskod 113 6 Molecular Molecular NNP cord-104231-fi8pskod 113 7 Probes Probes NNPS cord-104231-fi8pskod 113 8 , , , cord-104231-fi8pskod 113 9 Eugene Eugene NNP cord-104231-fi8pskod 113 10 , , , cord-104231-fi8pskod 113 11 OR OR NNP cord-104231-fi8pskod 113 12 ) ) -RRB- cord-104231-fi8pskod 113 13 was be VBD cord-104231-fi8pskod 113 14 dissolved dissolve VBN cord-104231-fi8pskod 113 15 in in IN cord-104231-fi8pskod 113 16 PBS PBS NNP cord-104231-fi8pskod 113 17 and and CC cord-104231-fi8pskod 113 18 used use VBN cord-104231-fi8pskod 113 19 at at IN cord-104231-fi8pskod 113 20 a a DT cord-104231-fi8pskod 113 21 final final JJ cord-104231-fi8pskod 113 22 concentration concentration NN cord-104231-fi8pskod 113 23 of of IN cord-104231-fi8pskod 113 24 20 20 CD cord-104231-fi8pskod 113 25 ~tg ~tg NNS cord-104231-fi8pskod 113 26 / / SYM cord-104231-fi8pskod 113 27 ml ml XX cord-104231-fi8pskod 113 28 ; ; : cord-104231-fi8pskod 113 29 ceils ceils NNP cord-104231-fi8pskod 113 30 were be VBD cord-104231-fi8pskod 113 31 incubated incubate VBN cord-104231-fi8pskod 113 32 in in IN cord-104231-fi8pskod 113 33 fresh fresh JJ cord-104231-fi8pskod 113 34 media medium NNS cord-104231-fi8pskod 113 35 containing contain VBG cord-104231-fi8pskod 113 36 the the DT cord-104231-fi8pskod 113 37 ligand ligand NN cord-104231-fi8pskod 113 38 for for IN cord-104231-fi8pskod 113 39 30 30 CD cord-104231-fi8pskod 113 40 rain rain NN cord-104231-fi8pskod 113 41 at at IN cord-104231-fi8pskod 113 42 37 37 CD cord-104231-fi8pskod 113 43 ° ° NN cord-104231-fi8pskod 113 44 C c NN cord-104231-fi8pskod 113 45 before before IN cord-104231-fi8pskod 113 46 washing wash VBG cord-104231-fi8pskod 113 47 with with IN cord-104231-fi8pskod 113 48 PBS PBS NNP cord-104231-fi8pskod 113 49 and and CC cord-104231-fi8pskod 113 50 fixation fixation NN cord-104231-fi8pskod 113 51 . . . cord-104231-fi8pskod 114 1 Slides slide NNS cord-104231-fi8pskod 114 2 were be VBD cord-104231-fi8pskod 114 3 examined examine VBN cord-104231-fi8pskod 114 4 on on IN cord-104231-fi8pskod 114 5 a a DT cord-104231-fi8pskod 114 6 Zeiss Zeiss NNP cord-104231-fi8pskod 114 7 Axiophot Axiophot NNP cord-104231-fi8pskod 114 8 fluorescence fluorescence NN cord-104231-fi8pskod 114 9 microscope microscope NN cord-104231-fi8pskod 114 10 using use VBG cord-104231-fi8pskod 114 11 a a DT cord-104231-fi8pskod 114 12 63 63 CD cord-104231-fi8pskod 114 13 ) ) -RRB- cord-104231-fi8pskod 114 14 < < XX cord-104231-fi8pskod 114 15 objective objective JJ cord-104231-fi8pskod 114 16 oil oil NN cord-104231-fi8pskod 114 17 immersion immersion NN cord-104231-fi8pskod 114 18 lens lens NN cord-104231-fi8pskod 114 19 and and CC cord-104231-fi8pskod 114 20 a a DT cord-104231-fi8pskod 114 21 10 10 CD cord-104231-fi8pskod 114 22 ) ) -RRB- cord-104231-fi8pskod 114 23 < < XX cord-104231-fi8pskod 114 24 eyepiece eyepiece NN cord-104231-fi8pskod 114 25 . . . cord-104231-fi8pskod 115 1 Transfected transfected JJ cord-104231-fi8pskod 115 2 cells cell NNS cord-104231-fi8pskod 115 3 were be VBD cord-104231-fi8pskod 115 4 fixed fix VBN cord-104231-fi8pskod 115 5 in in IN cord-104231-fi8pskod 115 6 2 2 CD cord-104231-fi8pskod 115 7 % % NN cord-104231-fi8pskod 115 8 paraformaldehyde/0.2 paraformaldehyde/0.2 CD cord-104231-fi8pskod 115 9 % % NN cord-104231-fi8pskod 115 10 glutaraldehyde glutaraldehyde VBN cord-104231-fi8pskod 115 11 in in IN cord-104231-fi8pskod 115 12 0.1 0.1 CD cord-104231-fi8pskod 115 13 M m NN cord-104231-fi8pskod 115 14 phosphate phosphate NN cord-104231-fi8pskod 115 15 buffer buffer NN cord-104231-fi8pskod 115 16 pH pH NNP cord-104231-fi8pskod 115 17 7.4 7.4 CD cord-104231-fi8pskod 115 18 for for IN cord-104231-fi8pskod 115 19 3 3 CD cord-104231-fi8pskod 115 20 h h NN cord-104231-fi8pskod 115 21 and and CC cord-104231-fi8pskod 115 22 processed process VBN cord-104231-fi8pskod 115 23 for for IN cord-104231-fi8pskod 115 24 cryo cryo NN cord-104231-fi8pskod 115 25 - - HYPH cord-104231-fi8pskod 115 26 immunoelectron immunoelectron NN cord-104231-fi8pskod 115 27 microscopy microscopy NN cord-104231-fi8pskod 115 28 essentially essentially RB cord-104231-fi8pskod 115 29 as as IN cord-104231-fi8pskod 115 30 described describe VBN cord-104231-fi8pskod 115 31 by by IN cord-104231-fi8pskod 115 32 Rabouille Rabouille NNP cord-104231-fi8pskod 115 33 et et NNP cord-104231-fi8pskod 115 34 al al NNP cord-104231-fi8pskod 115 35 . . . cord-104231-fi8pskod 116 1 ( ( -LRB- cord-104231-fi8pskod 116 2 1993 1993 CD cord-104231-fi8pskod 116 3 ) ) -RRB- cord-104231-fi8pskod 116 4 . . . cord-104231-fi8pskod 117 1 Cell cell NN cord-104231-fi8pskod 117 2 pellets pellet NNS cord-104231-fi8pskod 117 3 embedded embed VBN cord-104231-fi8pskod 117 4 in in IN cord-104231-fi8pskod 117 5 gelatin gelatin NN cord-104231-fi8pskod 117 6 were be VBD cord-104231-fi8pskod 117 7 cut cut VBN cord-104231-fi8pskod 117 8 into into IN cord-104231-fi8pskod 117 9 blocks block NNS cord-104231-fi8pskod 117 10 and and CC cord-104231-fi8pskod 117 11 infused infuse VBN cord-104231-fi8pskod 117 12 with with IN cord-104231-fi8pskod 117 13 2.3 2.3 CD cord-104231-fi8pskod 117 14 M M NNP cord-104231-fi8pskod 117 15 sucrose sucrose NN cord-104231-fi8pskod 117 16 overnight overnight RB cord-104231-fi8pskod 117 17 . . . cord-104231-fi8pskod 118 1 The the DT cord-104231-fi8pskod 118 2 blocks block NNS cord-104231-fi8pskod 118 3 were be VBD cord-104231-fi8pskod 118 4 frozen freeze VBN cord-104231-fi8pskod 118 5 in in IN cord-104231-fi8pskod 118 6 liquid liquid JJ cord-104231-fi8pskod 118 7 nitrogen nitrogen NN cord-104231-fi8pskod 118 8 , , , cord-104231-fi8pskod 118 9 ultrathin ultrathin JJ cord-104231-fi8pskod 118 10 cryosections cryosection NNS cord-104231-fi8pskod 118 11 were be VBD cord-104231-fi8pskod 118 12 cut cut VBN cord-104231-fi8pskod 118 13 and and CC cord-104231-fi8pskod 118 14 incubated incubate VBN cord-104231-fi8pskod 118 15 for for IN cord-104231-fi8pskod 118 16 30 30 CD cord-104231-fi8pskod 118 17 rain rain NN cord-104231-fi8pskod 118 18 with with IN cord-104231-fi8pskod 118 19 primary primary JJ cord-104231-fi8pskod 118 20 antibodies antibody NNS cord-104231-fi8pskod 118 21 . . . cord-104231-fi8pskod 119 1 Polyclonal Polyclonal NNP cord-104231-fi8pskod 119 2 rabbit rabbit NN cord-104231-fi8pskod 119 3 antiserum antiserum NN cord-104231-fi8pskod 119 4 to to IN cord-104231-fi8pskod 119 5 TGN38 tgn38 ADD cord-104231-fi8pskod 120 1 ( ( -LRB- cord-104231-fi8pskod 120 2 Lnzio Lnzio NNP cord-104231-fi8pskod 120 3 et et FW cord-104231-fi8pskod 120 4 al al NNP cord-104231-fi8pskod 120 5 . . NNP cord-104231-fi8pskod 120 6 , , , cord-104231-fi8pskod 120 7 1990 1990 CD cord-104231-fi8pskod 120 8 ) ) -RRB- cord-104231-fi8pskod 120 9 was be VBD cord-104231-fi8pskod 120 10 used use VBN cord-104231-fi8pskod 120 11 at at IN cord-104231-fi8pskod 120 12 a a DT cord-104231-fi8pskod 120 13 dilution dilution NN cord-104231-fi8pskod 120 14 of of IN cord-104231-fi8pskod 120 15 1:100 1:100 CD cord-104231-fi8pskod 120 16 and and CC cord-104231-fi8pskod 120 17 an an DT cord-104231-fi8pskod 120 18 affinity affinity NN cord-104231-fi8pskod 120 19 - - HYPH cord-104231-fi8pskod 120 20 purified purify VBN cord-104231-fi8pskod 120 21 rabbit rabbit NN cord-104231-fi8pskod 120 22 antibody antibody NN cord-104231-fi8pskod 120 23 to to IN cord-104231-fi8pskod 120 24 CD4 CD4 NNP cord-104231-fi8pskod 120 25 ( ( -LRB- cord-104231-fi8pskod 120 26 Dr. Dr. NNP cord-104231-fi8pskod 120 27 Mark Mark NNP cord-104231-fi8pskod 120 28 Marsh Marsh NNP cord-104231-fi8pskod 120 29 , , , cord-104231-fi8pskod 120 30 MRC MRC NNP cord-104231-fi8pskod 120 31 Centre Centre NNP cord-104231-fi8pskod 120 32 , , , cord-104231-fi8pskod 120 33 University University NNP cord-104231-fi8pskod 120 34 College College NNP cord-104231-fi8pskod 120 35 London London NNP cord-104231-fi8pskod 120 36 ) ) -RRB- cord-104231-fi8pskod 120 37 at at IN cord-104231-fi8pskod 120 38 a a DT cord-104231-fi8pskod 120 39 dilution dilution NN cord-104231-fi8pskod 120 40 of of IN cord-104231-fi8pskod 120 41 1:100 1:100 CD cord-104231-fi8pskod 120 42 . . . cord-104231-fi8pskod 121 1 Both both DT cord-104231-fi8pskod 121 2 were be VBD cord-104231-fi8pskod 121 3 diluted dilute VBN cord-104231-fi8pskod 121 4 into into IN cord-104231-fi8pskod 121 5 0.5 0.5 CD cord-104231-fi8pskod 121 6 % % NN cord-104231-fi8pskod 121 7 fish fish NN cord-104231-fi8pskod 121 8 skin skin NN cord-104231-fi8pskod 121 9 golatin golatin NNP cord-104231-fi8pskod 121 10 / / SYM cord-104231-fi8pskod 121 11 PBS PBS NNP cord-104231-fi8pskod 121 12 and and CC cord-104231-fi8pskod 121 13 the the DT cord-104231-fi8pskod 121 14 bound bind VBN cord-104231-fi8pskod 121 15 antibodies antibody NNS cord-104231-fi8pskod 121 16 were be VBD cord-104231-fi8pskod 121 17 detected detect VBN cord-104231-fi8pskod 121 18 using use VBG cord-104231-fi8pskod 121 19 protein protein NN cord-104231-fi8pskod 121 20 A A NNP cord-104231-fi8pskod 121 21 - - HYPH cord-104231-fi8pskod 121 22 coupled couple VBN cord-104231-fi8pskod 121 23 to to IN cord-104231-fi8pskod 121 24 10-nm 10-nm CD cord-104231-fi8pskod 121 25 gold gold NN cord-104231-fi8pskod 121 26 particles particle NNS cord-104231-fi8pskod 121 27 used use VBN cord-104231-fi8pskod 121 28 at at IN cord-104231-fi8pskod 121 29 a a DT cord-104231-fi8pskod 121 30 dilution dilution NN cord-104231-fi8pskod 121 31 of of IN cord-104231-fi8pskod 121 32 1:50 1:50 CD cord-104231-fi8pskod 121 33 ( ( -LRB- cord-104231-fi8pskod 121 34 from from IN cord-104231-fi8pskod 121 35 Cell Cell NNP cord-104231-fi8pskod 121 36 Biology Biology NNP cord-104231-fi8pskod 121 37 Department Department NNP cord-104231-fi8pskod 121 38 , , , cord-104231-fi8pskod 121 39 Ultrecht Ultrecht NNP cord-104231-fi8pskod 121 40 School School NNP cord-104231-fi8pskod 121 41 of of IN cord-104231-fi8pskod 121 42 Medicine Medicine NNP cord-104231-fi8pskod 121 43 , , , cord-104231-fi8pskod 121 44 Utrecht Utrecht NNP cord-104231-fi8pskod 121 45 , , , cord-104231-fi8pskod 121 46 Netherlands Netherlands NNP cord-104231-fi8pskod 121 47 ) ) -RRB- cord-104231-fi8pskod 121 48 . . . cord-104231-fi8pskod 122 1 The the DT cord-104231-fi8pskod 122 2 monoclonal monoclonal JJ cord-104231-fi8pskod 122 3 antibody antibody NN cord-104231-fi8pskod 122 4 14 14 CD cord-104231-fi8pskod 122 5 to to IN cord-104231-fi8pskod 122 6 CD8 CD8 NNP cord-104231-fi8pskod 122 7 ( ( -LRB- cord-104231-fi8pskod 122 8 Haynes Haynes NNP cord-104231-fi8pskod 122 9 , , , cord-104231-fi8pskod 122 10 1986 1986 CD cord-104231-fi8pskod 122 11 ) ) -RRB- cord-104231-fi8pskod 122 12 was be VBD cord-104231-fi8pskod 122 13 used use VBN cord-104231-fi8pskod 122 14 neat neat JJ cord-104231-fi8pskod 122 15 as as IN cord-104231-fi8pskod 122 16 a a DT cord-104231-fi8pskod 122 17 culture culture NN cord-104231-fi8pskod 122 18 supernatant supernatant JJ cord-104231-fi8pskod 122 19 and and CC cord-104231-fi8pskod 122 20 bound bind VBN cord-104231-fi8pskod 122 21 antibodies antibody NNS cord-104231-fi8pskod 122 22 were be VBD cord-104231-fi8pskod 122 23 detected detect VBN cord-104231-fi8pskod 122 24 using use VBG cord-104231-fi8pskod 122 25 a a DT cord-104231-fi8pskod 122 26 secondary secondary JJ cord-104231-fi8pskod 122 27 goat goat NN cord-104231-fi8pskod 122 28 anti anti JJ cord-104231-fi8pskod 122 29 - - JJ cord-104231-fi8pskod 122 30 mouse mouse JJ cord-104231-fi8pskod 122 31 antibody antibody NN cord-104231-fi8pskod 122 32 coupled couple VBN cord-104231-fi8pskod 122 33 to to IN cord-104231-fi8pskod 122 34 10-rim 10-rim CD cord-104231-fi8pskod 122 35 gold gold NN cord-104231-fi8pskod 122 36 particles particle NNS cord-104231-fi8pskod 122 37 at at IN cord-104231-fi8pskod 122 38 a a DT cord-104231-fi8pskod 122 39 dilution dilution NN cord-104231-fi8pskod 122 40 of of IN cord-104231-fi8pskod 122 41 1:20 1:20 CD cord-104231-fi8pskod 122 42 ( ( -LRB- cord-104231-fi8pskod 122 43 Nilsson Nilsson NNP cord-104231-fi8pskod 122 44 et et NNP cord-104231-fi8pskod 122 45 al al NNP cord-104231-fi8pskod 122 46 . . NNP cord-104231-fi8pskod 122 47 , , , cord-104231-fi8pskod 122 48 1994 1994 CD cord-104231-fi8pskod 122 49 ) ) -RRB- cord-104231-fi8pskod 122 50 . . . cord-104231-fi8pskod 123 1 Immunolabeling Immunolabeling NNP cord-104231-fi8pskod 123 2 was be VBD cord-104231-fi8pskod 123 3 followed follow VBN cord-104231-fi8pskod 123 4 by by IN cord-104231-fi8pskod 123 5 staining stain VBG cord-104231-fi8pskod 123 6 with with IN cord-104231-fi8pskod 123 7 uranyl uranyl NN cord-104231-fi8pskod 123 8 acetate acetate NN cord-104231-fi8pskod 123 9 and and CC cord-104231-fi8pskod 123 10 embedding embed VBG cord-104231-fi8pskod 123 11 in in IN cord-104231-fi8pskod 123 12 methyl methyl NN cord-104231-fi8pskod 123 13 cellulose cellulose NN cord-104231-fi8pskod 123 14 as as IN cord-104231-fi8pskod 123 15 described describe VBN cord-104231-fi8pskod 123 16 by by IN cord-104231-fi8pskod 123 17 Tokuyasu Tokuyasu NNP cord-104231-fi8pskod 123 18 ( ( -LRB- cord-104231-fi8pskod 123 19 1980 1980 CD cord-104231-fi8pskod 123 20 ) ) -RRB- cord-104231-fi8pskod 123 21 . . . cord-104231-fi8pskod 124 1 Quantitation quantitation NN cord-104231-fi8pskod 124 2 of of IN cord-104231-fi8pskod 124 3 immunolabeling immunolabele VBG cord-104231-fi8pskod 124 4 was be VBD cord-104231-fi8pskod 124 5 carried carry VBN cord-104231-fi8pskod 124 6 out out RP cord-104231-fi8pskod 124 7 by by IN cord-104231-fi8pskod 124 8 first first RB cord-104231-fi8pskod 124 9 identifying identify VBG cord-104231-fi8pskod 124 10 a a DT cord-104231-fi8pskod 124 11 labeled label VBN cord-104231-fi8pskod 124 12 area area NN cord-104231-fi8pskod 124 13 of of IN cord-104231-fi8pskod 124 14 the the DT cord-104231-fi8pskod 124 15 cell cell NN cord-104231-fi8pskod 124 16 where where WRB cord-104231-fi8pskod 124 17 profiles profile NNS cord-104231-fi8pskod 124 18 of of IN cord-104231-fi8pskod 124 19 the the DT cord-104231-fi8pskod 124 20 Crolgi Crolgi NNP cord-104231-fi8pskod 124 21 stack stack NN cord-104231-fi8pskod 124 22 and and CC cord-104231-fi8pskod 124 23 TGN TGN NNP cord-104231-fi8pskod 124 24 were be VBD cord-104231-fi8pskod 124 25 readily readily RB cord-104231-fi8pskod 124 26 visible visible JJ cord-104231-fi8pskod 124 27 . . . cord-104231-fi8pskod 125 1 The the DT cord-104231-fi8pskod 125 2 TGN TGN NNP cord-104231-fi8pskod 125 3 was be VBD cord-104231-fi8pskod 125 4 defined define VBN cord-104231-fi8pskod 125 5 morphologically morphologically RB cord-104231-fi8pskod 125 6 as as IN cord-104231-fi8pskod 125 7 described describe VBN cord-104231-fi8pskod 125 8 in in IN cord-104231-fi8pskod 125 9 the the DT cord-104231-fi8pskod 125 10 Results result NNS cord-104231-fi8pskod 125 11 . . . cord-104231-fi8pskod 126 1 Labeled label VBN cord-104231-fi8pskod 126 2 cell cell NN cord-104231-fi8pskod 126 3 sections section NNS cord-104231-fi8pskod 126 4 were be VBD cord-104231-fi8pskod 126 5 placed place VBN cord-104231-fi8pskod 126 6 in in IN cord-104231-fi8pskod 126 7 one one CD cord-104231-fi8pskod 126 8 of of IN cord-104231-fi8pskod 126 9 two two CD cord-104231-fi8pskod 126 10 categories category NNS cord-104231-fi8pskod 126 11 . . . cord-104231-fi8pskod 127 1 Cell cell NN cord-104231-fi8pskod 127 2 sections section NNS cord-104231-fi8pskod 127 3 in in IN cord-104231-fi8pskod 127 4 which which WDT cord-104231-fi8pskod 127 5 no no DT cord-104231-fi8pskod 127 6 eodosomes eodosome NNS cord-104231-fi8pskod 127 7 were be VBD cord-104231-fi8pskod 127 8 labeled label VBN cord-104231-fi8pskod 127 9 consistently consistently RB cord-104231-fi8pskod 127 10 contained contain VBN cord-104231-fi8pskod 127 11 fewer few JJR cord-104231-fi8pskod 127 12 than than IN cord-104231-fi8pskod 127 13 50 50 CD cord-104231-fi8pskod 127 14 - - SYM cord-104231-fi8pskod 127 15 60 60 CD cord-104231-fi8pskod 127 16 gold gold NN cord-104231-fi8pskod 127 17 particles particle NNS cord-104231-fi8pskod 127 18 whereas whereas IN cord-104231-fi8pskod 127 19 cell cell NN cord-104231-fi8pskod 127 20 sections section NNS cord-104231-fi8pskod 127 21 containing contain VBG cord-104231-fi8pskod 127 22 labeled label VBN cord-104231-fi8pskod 127 23 endosomes endosome NNS cord-104231-fi8pskod 127 24 contained contain VBD cord-104231-fi8pskod 127 25 more more JJR cord-104231-fi8pskod 127 26 than than IN cord-104231-fi8pskod 127 27 50 50 CD cord-104231-fi8pskod 127 28 - - SYM cord-104231-fi8pskod 127 29 60 60 CD cord-104231-fi8pskod 127 30 gold gold NN cord-104231-fi8pskod 127 31 particles particle NNS cord-104231-fi8pskod 127 32 . . . cord-104231-fi8pskod 128 1 In in IN cord-104231-fi8pskod 128 2 each each DT cord-104231-fi8pskod 128 3 experiment experiment NN cord-104231-fi8pskod 128 4 , , , cord-104231-fi8pskod 128 5 200 200 CD cord-104231-fi8pskod 128 6 - - SYM cord-104231-fi8pskod 128 7 1,000 1,000 CD cord-104231-fi8pskod 128 8 gold gold NN cord-104231-fi8pskod 128 9 particles particle NNS cord-104231-fi8pskod 128 10 were be VBD cord-104231-fi8pskod 128 11 counted count VBN cord-104231-fi8pskod 128 12 depending depend VBG cord-104231-fi8pskod 128 13 on on IN cord-104231-fi8pskod 128 14 the the DT cord-104231-fi8pskod 128 15 type type NN cord-104231-fi8pskod 128 16 of of IN cord-104231-fi8pskod 128 17 construct construct NN cord-104231-fi8pskod 128 18 used use VBN cord-104231-fi8pskod 128 19 for for IN cord-104231-fi8pskod 128 20 transfection transfection NN cord-104231-fi8pskod 128 21 ( ( -LRB- cord-104231-fi8pskod 128 22 see see VB cord-104231-fi8pskod 128 23 Fig Fig NNP cord-104231-fi8pskod 128 24 . . . cord-104231-fi8pskod 128 25 6 6 CD cord-104231-fi8pskod 128 26 legend legend NN cord-104231-fi8pskod 128 27 ) ) -RRB- cord-104231-fi8pskod 128 28 . . . cord-104231-fi8pskod 129 1 The the DT cord-104231-fi8pskod 129 2 background background NN cord-104231-fi8pskod 129 3 observed observe VBD cord-104231-fi8pskod 129 4 in in IN cord-104231-fi8pskod 129 5 each each DT cord-104231-fi8pskod 129 6 experiment experiment NN cord-104231-fi8pskod 129 7 was be VBD cord-104231-fi8pskod 129 8 extremely extremely RB cord-104231-fi8pskod 129 9 low low JJ cord-104231-fi8pskod 129 10 and and CC cord-104231-fi8pskod 129 11 therefore therefore RB cord-104231-fi8pskod 129 12 ignored ignore VBN cord-104231-fi8pskod 129 13 . . . cord-104231-fi8pskod 130 1 The the DT cord-104231-fi8pskod 130 2 relative relative JJ cord-104231-fi8pskod 130 3 distribution distribution NN cord-104231-fi8pskod 130 4 of of IN cord-104231-fi8pskod 130 5 immunolabeling immunolabeling NN cord-104231-fi8pskod 130 6 was be VBD cord-104231-fi8pskod 130 7 performed perform VBN cord-104231-fi8pskod 130 8 by by IN cord-104231-fi8pskod 130 9 counting count VBG cord-104231-fi8pskod 130 10 the the DT cord-104231-fi8pskod 130 11 gold gold NN cord-104231-fi8pskod 130 12 particles particle NNS cord-104231-fi8pskod 130 13 over over IN cord-104231-fi8pskod 130 14 the the DT cord-104231-fi8pskod 130 15 membranes membrane NNS cord-104231-fi8pskod 130 16 ( ( -LRB- cord-104231-fi8pskod 130 17 Golgi Golgi NNP cord-104231-fi8pskod 130 18 stack stack NN cord-104231-fi8pskod 130 19 , , , cord-104231-fi8pskod 130 20 TGN TGN NNP cord-104231-fi8pskod 130 21 , , , cord-104231-fi8pskod 130 22 endosornes endosorne NNS cord-104231-fi8pskod 130 23 , , , cord-104231-fi8pskod 130 24 and and CC cord-104231-fi8pskod 130 25 plasma plasma NN cord-104231-fi8pskod 130 26 membrane membrane NN cord-104231-fi8pskod 130 27 ) ) -RRB- cord-104231-fi8pskod 130 28 of of IN cord-104231-fi8pskod 130 29 selected select VBN cord-104231-fi8pskod 130 30 cells cell NNS cord-104231-fi8pskod 130 31 . . . cord-104231-fi8pskod 131 1 The the DT cord-104231-fi8pskod 131 2 linear linear JJ cord-104231-fi8pskod 131 3 density density NN cord-104231-fi8pskod 131 4 of of IN cord-104231-fi8pskod 131 5 the the DT cord-104231-fi8pskod 131 6 gold gold NN cord-104231-fi8pskod 131 7 particles particle NNS cord-104231-fi8pskod 131 8 at at IN cord-104231-fi8pskod 131 9 the the DT cord-104231-fi8pskod 131 10 plasma plasma NN cord-104231-fi8pskod 131 11 membrane membrane NN cord-104231-fi8pskod 131 12 was be VBD cord-104231-fi8pskod 131 13 estimated estimate VBN cord-104231-fi8pskod 131 14 by by IN cord-104231-fi8pskod 131 15 the the DT cord-104231-fi8pskod 131 16 point point NN cord-104231-fi8pskod 131 17 hit hit VBN cord-104231-fi8pskod 131 18 method method NN cord-104231-fi8pskod 131 19 . . . cord-104231-fi8pskod 132 1 Briefly briefly RB cord-104231-fi8pskod 132 2 , , , cord-104231-fi8pskod 132 3 pictures picture NNS cord-104231-fi8pskod 132 4 were be VBD cord-104231-fi8pskod 132 5 printed print VBN cord-104231-fi8pskod 132 6 at at IN cord-104231-fi8pskod 132 7 38,750× 38,750× CD cord-104231-fi8pskod 132 8 or or CC cord-104231-fi8pskod 132 9 52,000× 52,000× CD cord-104231-fi8pskod 132 10 final final JJ cord-104231-fi8pskod 132 11 magnification magnification NN cord-104231-fi8pskod 132 12 and and CC cord-104231-fi8pskod 132 13 overlaid overlay VBN cord-104231-fi8pskod 132 14 with with IN cord-104231-fi8pskod 132 15 a a DT cord-104231-fi8pskod 132 16 grid grid NN cord-104231-fi8pskod 132 17 containing contain VBG cord-104231-fi8pskod 132 18 a a DT cord-104231-fi8pskod 132 19 point point NN cord-104231-fi8pskod 132 20 - - HYPH cord-104231-fi8pskod 132 21 to to IN cord-104231-fi8pskod 132 22 - - HYPH cord-104231-fi8pskod 132 23 point point NN cord-104231-fi8pskod 132 24 spacing spacing NN cord-104231-fi8pskod 132 25 of of IN cord-104231-fi8pskod 132 26 2 2 CD cord-104231-fi8pskod 132 27 crn crn NN cord-104231-fi8pskod 132 28 ( ( -LRB- cord-104231-fi8pskod 132 29 plasma plasma NN cord-104231-fi8pskod 132 30 membrane membrane NN cord-104231-fi8pskod 132 31 ) ) -RRB- cord-104231-fi8pskod 132 32 or or CC cord-104231-fi8pskod 132 33 0.5 0.5 CD cord-104231-fi8pskod 132 34 cm cm NN cord-104231-fi8pskod 132 35 ( ( -LRB- cord-104231-fi8pskod 132 36 TGN TGN NNP cord-104231-fi8pskod 132 37 ) ) -RRB- cord-104231-fi8pskod 132 38 ( ( -LRB- cord-104231-fi8pskod 132 39 d d NN cord-104231-fi8pskod 132 40 ) ) -RRB- cord-104231-fi8pskod 132 41 . . . cord-104231-fi8pskod 133 1 The the DT cord-104231-fi8pskod 133 2 number number NN cord-104231-fi8pskod 133 3 of of IN cord-104231-fi8pskod 133 4 intersections intersection NNS cord-104231-fi8pskod 133 5 ( ( -LRB- cord-104231-fi8pskod 133 6 I i NN cord-104231-fi8pskod 133 7 ) ) -RRB- cord-104231-fi8pskod 133 8 of of IN cord-104231-fi8pskod 133 9 the the DT cord-104231-fi8pskod 133 10 membrane membrane NN cord-104231-fi8pskod 133 11 with with IN cord-104231-fi8pskod 133 12 this this DT cord-104231-fi8pskod 133 13 grid grid NN cord-104231-fi8pskod 133 14 were be VBD cord-104231-fi8pskod 133 15 counted count VBN cord-104231-fi8pskod 133 16 . . . cord-104231-fi8pskod 134 1 The the DT cord-104231-fi8pskod 134 2 total total JJ cord-104231-fi8pskod 134 3 number number NN cord-104231-fi8pskod 134 4 of of IN cord-104231-fi8pskod 134 5 gold gold NN cord-104231-fi8pskod 134 6 particles particle NNS cord-104231-fi8pskod 134 7 over over IN cord-104231-fi8pskod 134 8 the the DT cord-104231-fi8pskod 134 9 total total JJ cord-104231-fi8pskod 134 10 membrane membrane NN cord-104231-fi8pskod 134 11 was be VBD cord-104231-fi8pskod 134 12 counted count VBN cord-104231-fi8pskod 134 13 and and CC cord-104231-fi8pskod 134 14 the the DT cord-104231-fi8pskod 134 15 linear linear JJ cord-104231-fi8pskod 134 16 density density NN cord-104231-fi8pskod 134 17 was be VBD cord-104231-fi8pskod 134 18 then then RB cord-104231-fi8pskod 134 19 calculated calculate VBN cord-104231-fi8pskod 134 20 using use VBG cord-104231-fi8pskod 134 21 the the DT cord-104231-fi8pskod 134 22 formula:-Gold formula:-gold NN cord-104231-fi8pskod 134 23 particles/(I particles/(I NNP cord-104231-fi8pskod 134 24 X X NNP cord-104231-fi8pskod 134 25 d d NNP cord-104231-fi8pskod 134 26 / / SYM cord-104231-fi8pskod 134 27 magnification magnification NN cord-104231-fi8pskod 134 28 ) ) -RRB- cord-104231-fi8pskod 134 29 . . . cord-104231-fi8pskod 135 1 Transfected transfected JJ cord-104231-fi8pskod 135 2 cells cell NNS cord-104231-fi8pskod 135 3 were be VBD cord-104231-fi8pskod 135 4 grown grow VBN cord-104231-fi8pskod 135 5 in in IN cord-104231-fi8pskod 135 6 30-ram 30-ram CD cord-104231-fi8pskod 135 7 wells well NNS cord-104231-fi8pskod 135 8 and and CC cord-104231-fi8pskod 135 9 processed process VBN cord-104231-fi8pskod 135 10 for for IN cord-104231-fi8pskod 135 11 FACS FACS NNP cord-104231-fi8pskod 135 12 analysis analysis NN cord-104231-fi8pskod 135 13 exactly exactly RB cord-104231-fi8pskod 135 14 as as IN cord-104231-fi8pskod 135 15 described describe VBN cord-104231-fi8pskod 135 16 by by IN cord-104231-fi8pskod 135 17 Nilsson Nilsson NNP cord-104231-fi8pskod 135 18 et et NNP cord-104231-fi8pskod 135 19 al al NNP cord-104231-fi8pskod 135 20 . . . cord-104231-fi8pskod 136 1 ( ( -LRB- cord-104231-fi8pskod 136 2 1989 1989 CD cord-104231-fi8pskod 136 3 ) ) -RRB- cord-104231-fi8pskod 136 4 . . . cord-104231-fi8pskod 137 1 Native native JJ cord-104231-fi8pskod 137 2 and and CC cord-104231-fi8pskod 137 3 hybrid hybrid JJ cord-104231-fi8pskod 137 4 CD8 CD8 NNP cord-104231-fi8pskod 137 5 proteins protein NNS cord-104231-fi8pskod 137 6 were be VBD cord-104231-fi8pskod 137 7 detected detect VBN cord-104231-fi8pskod 137 8 using use VBG cord-104231-fi8pskod 137 9 the the DT cord-104231-fi8pskod 137 10 culture culture NN cord-104231-fi8pskod 137 11 supernatant supernatant VBN cord-104231-fi8pskod 137 12 from from IN cord-104231-fi8pskod 137 13 the the DT cord-104231-fi8pskod 137 14 OKT8 OKT8 NNP cord-104231-fi8pskod 137 15 hybridoma hybridoma NN cord-104231-fi8pskod 137 16 . . . cord-104231-fi8pskod 138 1 Approximately approximately RB cord-104231-fi8pskod 138 2 5 5 CD cord-104231-fi8pskod 138 3 × × NN cord-104231-fi8pskod 138 4 105 105 CD cord-104231-fi8pskod 138 5 cells cell NNS cord-104231-fi8pskod 138 6 were be VBD cord-104231-fi8pskod 138 7 incubated incubate VBN cord-104231-fi8pskod 138 8 with with IN cord-104231-fi8pskod 138 9 the the DT cord-104231-fi8pskod 138 10 primary primary JJ cord-104231-fi8pskod 138 11 antibodies antibody NNS cord-104231-fi8pskod 138 12 on on IN cord-104231-fi8pskod 138 13 ice ice NN cord-104231-fi8pskod 138 14 for for IN cord-104231-fi8pskod 138 15 20 20 CD cord-104231-fi8pskod 138 16 rain rain NN cord-104231-fi8pskod 138 17 followed follow VBN cord-104231-fi8pskod 138 18 by by IN cord-104231-fi8pskod 138 19 gentle gentle JJ cord-104231-fi8pskod 138 20 washing washing NN cord-104231-fi8pskod 138 21 . . . cord-104231-fi8pskod 139 1 The the DT cord-104231-fi8pskod 139 2 secondary secondary JJ cord-104231-fi8pskod 139 3 antibody antibody NN cord-104231-fi8pskod 139 4 was be VBD cord-104231-fi8pskod 139 5 FITC FITC NNP cord-104231-fi8pskod 139 6 - - HYPH cord-104231-fi8pskod 139 7 conjugated conjugate VBN cord-104231-fi8pskod 139 8 sheep sheep NN cord-104231-fi8pskod 139 9 anti anti NN cord-104231-fi8pskod 139 10 - - JJ cord-104231-fi8pskod 139 11 mouse mouse JJ cord-104231-fi8pskod 139 12 ( ( -LRB- cord-104231-fi8pskod 139 13 Vector Vector NNP cord-104231-fi8pskod 139 14 Labs Labs NNP cord-104231-fi8pskod 139 15 . . . cord-104231-fi8pskod 140 1 , , , cord-104231-fi8pskod 140 2 Burlingame Burlingame NNP cord-104231-fi8pskod 140 3 , , , cord-104231-fi8pskod 140 4 CA CA NNP cord-104231-fi8pskod 140 5 ) ) -RRB- cord-104231-fi8pskod 140 6 used use VBD cord-104231-fi8pskod 140 7 at at IN cord-104231-fi8pskod 140 8 a a DT cord-104231-fi8pskod 140 9 dilution dilution NN cord-104231-fi8pskod 140 10 of of IN cord-104231-fi8pskod 140 11 1:100 1:100 CD cord-104231-fi8pskod 140 12 . . . cord-104231-fi8pskod 141 1 After after IN cord-104231-fi8pskod 141 2 washing washing NN cord-104231-fi8pskod 141 3 , , , cord-104231-fi8pskod 141 4 cells cell NNS cord-104231-fi8pskod 141 5 were be VBD cord-104231-fi8pskod 141 6 fixed fix VBN cord-104231-fi8pskod 141 7 in in IN cord-104231-fi8pskod 141 8 3 3 CD cord-104231-fi8pskod 141 9 % % NN cord-104231-fi8pskod 141 10 paraformaldehyde paraformaldehyde NNP cord-104231-fi8pskod 141 11 / / SYM cord-104231-fi8pskod 141 12 PBS PBS NNP cord-104231-fi8pskod 141 13 for for IN cord-104231-fi8pskod 141 14 20 20 CD cord-104231-fi8pskod 141 15 rain rain NN cord-104231-fi8pskod 141 16 on on IN cord-104231-fi8pskod 141 17 ice ice NN cord-104231-fi8pskod 141 18 . . . cord-104231-fi8pskod 142 1 Immediately immediately RB cord-104231-fi8pskod 142 2 before before IN cord-104231-fi8pskod 142 3 FACS FACS NNP cord-104231-fi8pskod 142 4 analysis analysis NN cord-104231-fi8pskod 142 5 , , , cord-104231-fi8pskod 142 6 cells cell NNS cord-104231-fi8pskod 142 7 were be VBD cord-104231-fi8pskod 142 8 washed wash VBN cord-104231-fi8pskod 142 9 three three CD cord-104231-fi8pskod 142 10 times time NNS cord-104231-fi8pskod 142 11 with with IN cord-104231-fi8pskod 142 12 calcium calcium NN cord-104231-fi8pskod 142 13 / / SYM cord-104231-fi8pskod 142 14 magnesium magnesium NN cord-104231-fi8pskod 142 15 - - HYPH cord-104231-fi8pskod 142 16 free free JJ cord-104231-fi8pskod 142 17 PBS PBS NNP cord-104231-fi8pskod 142 18 and and CC cord-104231-fi8pskod 142 19 resuspended resuspend VBD cord-104231-fi8pskod 142 20 in in IN cord-104231-fi8pskod 142 21 0.5 0.5 CD cord-104231-fi8pskod 142 22 ml ml NNS cord-104231-fi8pskod 142 23 of of IN cord-104231-fi8pskod 142 24 this this DT cord-104231-fi8pskod 142 25 solution solution NN cord-104231-fi8pskod 142 26 . . . cord-104231-fi8pskod 143 1 A a DT cord-104231-fi8pskod 143 2 Becton Becton NNP cord-104231-fi8pskod 143 3 - - HYPH cord-104231-fi8pskod 143 4 Dickinson Dickinson NNP cord-104231-fi8pskod 143 5 FACScan facscan NN cord-104231-fi8pskod 143 6 440 440 CD cord-104231-fi8pskod 143 7 machine machine NN cord-104231-fi8pskod 143 8 was be VBD cord-104231-fi8pskod 143 9 used use VBN cord-104231-fi8pskod 143 10 to to TO cord-104231-fi8pskod 143 11 analyze analyze VB cord-104231-fi8pskod 143 12 the the DT cord-104231-fi8pskod 143 13 samples sample NNS cord-104231-fi8pskod 143 14 . . . cord-104231-fi8pskod 144 1 Quantitation Quantitation NNP cord-104231-fi8pskod 144 2 of of IN cord-104231-fi8pskod 144 3 FAnS FAnS NNP cord-104231-fi8pskod 144 4 data datum NNS cord-104231-fi8pskod 144 5 was be VBD cord-104231-fi8pskod 144 6 performed perform VBN cord-104231-fi8pskod 144 7 by by IN cord-104231-fi8pskod 144 8 calculating calculate VBG cord-104231-fi8pskod 144 9 two two CD cord-104231-fi8pskod 144 10 parameters parameter NNS cord-104231-fi8pskod 144 11 : : : cord-104231-fi8pskod 144 12 the the DT cord-104231-fi8pskod 144 13 amount amount NN cord-104231-fi8pskod 144 14 of of IN cord-104231-fi8pskod 144 15 positive positive JJ cord-104231-fi8pskod 144 16 cells cell NNS cord-104231-fi8pskod 144 17 expressing express VBG cord-104231-fi8pskod 144 18 each each DT cord-104231-fi8pskod 144 19 protein protein NN cord-104231-fi8pskod 144 20 and and CC cord-104231-fi8pskod 144 21 the the DT cord-104231-fi8pskod 144 22 arithmetic arithmetic JJ cord-104231-fi8pskod 144 23 mean mean JJ cord-104231-fi8pskod 144 24 intensity intensity NN cord-104231-fi8pskod 144 25 of of IN cord-104231-fi8pskod 144 26 fluorescence fluorescence NN cord-104231-fi8pskod 144 27 of of IN cord-104231-fi8pskod 144 28 these these DT cord-104231-fi8pskod 144 29 positive positive JJ cord-104231-fi8pskod 144 30 cells cell NNS cord-104231-fi8pskod 144 31 ( ( -LRB- cord-104231-fi8pskod 144 32 ~:Ni ~:Ni NNS cord-104231-fi8pskod 144 33 . . . cord-104231-fi8pskod 145 1 Ii2/~Nili Ii2/~Nili NNP cord-104231-fi8pskod 145 2 ) ) -RRB- cord-104231-fi8pskod 145 3 . . . cord-104231-fi8pskod 146 1 The the DT cord-104231-fi8pskod 146 2 mean mean JJ cord-104231-fi8pskod 146 3 intensity intensity NN cord-104231-fi8pskod 146 4 was be VBD cord-104231-fi8pskod 146 5 multiplied multiply VBN cord-104231-fi8pskod 146 6 by by IN cord-104231-fi8pskod 146 7 the the DT cord-104231-fi8pskod 146 8 number number NN cord-104231-fi8pskod 146 9 of of IN cord-104231-fi8pskod 146 10 positive positive JJ cord-104231-fi8pskod 146 11 cells cell NNS cord-104231-fi8pskod 146 12 which which WDT cord-104231-fi8pskod 146 13 gave give VBD cord-104231-fi8pskod 146 14 the the DT cord-104231-fi8pskod 146 15 total total JJ cord-104231-fi8pskod 146 16 fluorescence fluorescence NN cord-104231-fi8pskod 146 17 intensity intensity NN cord-104231-fi8pskod 146 18 ( ( -LRB- cord-104231-fi8pskod 146 19 arbitrary arbitrary JJ cord-104231-fi8pskod 146 20 units unit NNS cord-104231-fi8pskod 146 21 ) ) -RRB- cord-104231-fi8pskod 146 22 detected detect VBD cord-104231-fi8pskod 146 23 for for IN cord-104231-fi8pskod 146 24 the the DT cord-104231-fi8pskod 146 25 positive positive JJ cord-104231-fi8pskod 146 26 cell cell NN cord-104231-fi8pskod 146 27 population population NN cord-104231-fi8pskod 146 28 . . . cord-104231-fi8pskod 147 1 The the DT cord-104231-fi8pskod 147 2 total total JJ cord-104231-fi8pskod 147 3 fluorescence fluorescence NN cord-104231-fi8pskod 147 4 intensity intensity NN cord-104231-fi8pskod 147 5 of of IN cord-104231-fi8pskod 147 6 the the DT cord-104231-fi8pskod 147 7 CD8 CD8 NNP cord-104231-fi8pskod 147 8 positive positive JJ cord-104231-fi8pskod 147 9 cells cell NNS cord-104231-fi8pskod 147 10 was be VBD cord-104231-fi8pskod 147 11 represented represent VBN cord-104231-fi8pskod 147 12 as as IN cord-104231-fi8pskod 147 13 100 100 CD cord-104231-fi8pskod 147 14 % % NN cord-104231-fi8pskod 147 15 and and CC cord-104231-fi8pskod 147 16 the the DT cord-104231-fi8pskod 147 17 total total JJ cord-104231-fi8pskod 147 18 fluorescence fluorescence NN cord-104231-fi8pskod 147 19 intensity intensity NN cord-104231-fi8pskod 147 20 of of IN cord-104231-fi8pskod 147 21 positively positively RB cord-104231-fi8pskod 147 22 gated gate VBN cord-104231-fi8pskod 147 23 cells cell NNS cord-104231-fi8pskod 147 24 expressing express VBG cord-104231-fi8pskod 147 25 other other JJ cord-104231-fi8pskod 147 26 CD8 CD8 NNP cord-104231-fi8pskod 147 27 hybrid hybrid NN cord-104231-fi8pskod 147 28 proteins protein NNS cord-104231-fi8pskod 147 29 were be VBD cord-104231-fi8pskod 147 30 expressed express VBN cord-104231-fi8pskod 147 31 as as IN cord-104231-fi8pskod 147 32 a a DT cord-104231-fi8pskod 147 33 percentage percentage NN cord-104231-fi8pskod 147 34 of of IN cord-104231-fi8pskod 147 35 total total JJ cord-104231-fi8pskod 147 36 CD8 CD8 NNP cord-104231-fi8pskod 147 37 . . . cord-104231-fi8pskod 148 1 Immunopreeipitation Immunopreeipitation NNP cord-104231-fi8pskod 148 2 was be VBD cord-104231-fi8pskod 148 3 carried carry VBN cord-104231-fi8pskod 148 4 out out RP cord-104231-fi8pskod 148 5 essentially essentially RB cord-104231-fi8pskod 148 6 as as IN cord-104231-fi8pskod 148 7 described describe VBN cord-104231-fi8pskod 148 8 by by IN cord-104231-fi8pskod 148 9 Jackson Jackson NNP cord-104231-fi8pskod 148 10 et et NNP cord-104231-fi8pskod 148 11 al al NNP cord-104231-fi8pskod 148 12 . . . cord-104231-fi8pskod 149 1 ( ( -LRB- cord-104231-fi8pskod 149 2 1989 1989 CD cord-104231-fi8pskod 149 3 ) ) -RRB- cord-104231-fi8pskod 149 4 . . . cord-104231-fi8pskod 150 1 Transfected transfecte VBN cord-104231-fi8pskod 150 2 cells cell NNS cord-104231-fi8pskod 150 3 in in IN cord-104231-fi8pskod 150 4 100-mm 100-mm CD cord-104231-fi8pskod 150 5 dishes dish NNS cord-104231-fi8pskod 150 6 were be VBD cord-104231-fi8pskod 150 7 labeled label VBN cord-104231-fi8pskod 150 8 with with IN cord-104231-fi8pskod 150 9 0.2 0.2 CD cord-104231-fi8pskod 150 10 rnCi rnci XX cord-104231-fi8pskod 150 11 [ [ -LRB- cord-104231-fi8pskod 150 12 35S]methionine 35s]methionine NN cord-104231-fi8pskod 150 13 / / SYM cord-104231-fi8pskod 150 14 cysteine cysteine NN cord-104231-fi8pskod 150 15 for for IN cord-104231-fi8pskod 150 16 20 20 CD cord-104231-fi8pskod 150 17 rain rain NN cord-104231-fi8pskod 150 18 and and CC cord-104231-fi8pskod 150 19 either either CC cord-104231-fi8pskod 150 20 lysed lyse VBN cord-104231-fi8pskod 150 21 in in IN cord-104231-fi8pskod 150 22 detergent detergent NN cord-104231-fi8pskod 150 23 immediately immediately RB cord-104231-fi8pskod 150 24 or or CC cord-104231-fi8pskod 150 25 chased chase VBN cord-104231-fi8pskod 150 26 with with IN cord-104231-fi8pskod 150 27 medium medium NN cord-104231-fi8pskod 150 28 containing contain VBG cord-104231-fi8pskod 150 29 excess excess JJ cord-104231-fi8pskod 150 30 cold cold JJ cord-104231-fi8pskod 150 31 methionine methionine NN cord-104231-fi8pskod 150 32 and and CC cord-104231-fi8pskod 150 33 cysteine cysteine NN cord-104231-fi8pskod 150 34 . . . cord-104231-fi8pskod 151 1 After after IN cord-104231-fi8pskod 151 2 15 15 CD cord-104231-fi8pskod 151 3 rain rain NN cord-104231-fi8pskod 151 4 on on IN cord-104231-fi8pskod 151 5 ice ice NN cord-104231-fi8pskod 151 6 the the DT cord-104231-fi8pskod 151 7 cells cell NNS cord-104231-fi8pskod 151 8 were be VBD cord-104231-fi8pskod 151 9 scraped scrape VBN cord-104231-fi8pskod 151 10 off off RP cord-104231-fi8pskod 151 11 and and CC cord-104231-fi8pskod 151 12 lysates lysate NNS cord-104231-fi8pskod 151 13 centrifuged centrifuge VBN cord-104231-fi8pskod 151 14 at at IN cord-104231-fi8pskod 151 15 medium medium NN cord-104231-fi8pskod 151 16 ( ( -LRB- cord-104231-fi8pskod 151 17 12,000 12,000 CD cord-104231-fi8pskod 151 18 g g NN cord-104231-fi8pskod 151 19 , , , cord-104231-fi8pskod 151 20 10 10 CD cord-104231-fi8pskod 151 21 min min NN cord-104231-fi8pskod 151 22 at at IN cord-104231-fi8pskod 151 23 4 4 CD cord-104231-fi8pskod 151 24 ° ° NN cord-104231-fi8pskod 151 25 C c NN cord-104231-fi8pskod 151 26 ) ) -RRB- cord-104231-fi8pskod 151 27 and and CC cord-104231-fi8pskod 151 28 high high JJ cord-104231-fi8pskod 151 29 speed speed NN cord-104231-fi8pskod 151 30 ( ( -LRB- cord-104231-fi8pskod 151 31 400000 400000 CD cord-104231-fi8pskod 151 32 g g NN cord-104231-fi8pskod 151 33 , , , cord-104231-fi8pskod 151 34 30 30 CD cord-104231-fi8pskod 151 35 rain rain NN cord-104231-fi8pskod 151 36 at at IN cord-104231-fi8pskod 151 37 40C 40c CD cord-104231-fi8pskod 151 38 ) ) -RRB- cord-104231-fi8pskod 151 39 to to TO cord-104231-fi8pskod 151 40 remove remove VB cord-104231-fi8pskod 151 41 particulate particulate NN cord-104231-fi8pskod 151 42 material material NN cord-104231-fi8pskod 151 43 . . . cord-104231-fi8pskod 152 1 The the DT cord-104231-fi8pskod 152 2 supernatants supernatant NNS cord-104231-fi8pskod 152 3 were be VBD cord-104231-fi8pskod 152 4 precleared precleare VBN cord-104231-fi8pskod 152 5 by by IN cord-104231-fi8pskod 152 6 incubation incubation NN cord-104231-fi8pskod 152 7 with with IN cord-104231-fi8pskod 152 8 mouse mouse NN cord-104231-fi8pskod 152 9 IgG IgG NNP cord-104231-fi8pskod 152 10 bound bind VBN cord-104231-fi8pskod 152 11 to to TO cord-104231-fi8pskod 152 12 protein protein VB cord-104231-fi8pskod 152 13 A A NNP cord-104231-fi8pskod 152 14 - - : cord-104231-fi8pskod 152 15 Sepharose Sepharose NNP cord-104231-fi8pskod 152 16 ( ( -LRB- cord-104231-fi8pskod 152 17 Pharmacia Pharmacia NNP cord-104231-fi8pskod 152 18 Corp. Corp. NNP cord-104231-fi8pskod 152 19 , , , cord-104231-fi8pskod 152 20 Uppsala Uppsala NNP cord-104231-fi8pskod 152 21 , , , cord-104231-fi8pskod 152 22 Sweden Sweden NNP cord-104231-fi8pskod 152 23 ) ) -RRB- cord-104231-fi8pskod 152 24 and and CC cord-104231-fi8pskod 152 25 native native JJ cord-104231-fi8pskod 152 26 and and CC cord-104231-fi8pskod 152 27 hybrid hybrid JJ cord-104231-fi8pskod 152 28 proteins protein NNS cord-104231-fi8pskod 153 1 were be VBD cord-104231-fi8pskod 153 2 immunoprecipitated immunoprecipitate VBN cord-104231-fi8pskod 153 3 using use VBG cord-104231-fi8pskod 153 4 QS4120 QS4120 NNP cord-104231-fi8pskod 153 5 ( ( -LRB- cord-104231-fi8pskod 153 6 anti anti NNP cord-104231-fi8pskod 153 7 - - NNP cord-104231-fi8pskod 153 8 CD4 CD4 NNP cord-104231-fi8pskod 153 9 ) ) -RRB- cord-104231-fi8pskod 153 10 or or CC cord-104231-fi8pskod 153 11 OKT8 OKT8 NNP cord-104231-fi8pskod 153 12 ( ( -LRB- cord-104231-fi8pskod 153 13 anti anti JJ cord-104231-fi8pskod 153 14 - - JJ cord-104231-fi8pskod 153 15 CDS cds JJ cord-104231-fi8pskod 153 16 ) ) -RRB- cord-104231-fi8pskod 153 17 antibody antibody NN cord-104231-fi8pskod 153 18 bound bind VBN cord-104231-fi8pskod 153 19 to to TO cord-104231-fi8pskod 153 20 protein protein VB cord-104231-fi8pskod 153 21 A A NNP cord-104231-fi8pskod 153 22 - - HYPH cord-104231-fi8pskod 153 23 Sepharose Sepharose NNP cord-104231-fi8pskod 153 24 . . . cord-104231-fi8pskod 154 1 Immunoprecipitates immunoprecipitate NNS cord-104231-fi8pskod 154 2 were be VBD cord-104231-fi8pskod 154 3 then then RB cord-104231-fi8pskod 154 4 washed wash VBN cord-104231-fi8pskod 154 5 sequentially sequentially RB cord-104231-fi8pskod 154 6 twice twice RB cord-104231-fi8pskod 154 7 with with IN cord-104231-fi8pskod 154 8 lysis lysis NN cord-104231-fi8pskod 154 9 buffer buffer NN cord-104231-fi8pskod 154 10 , , , cord-104231-fi8pskod 154 11 once once RB cord-104231-fi8pskod 154 12 with with IN cord-104231-fi8pskod 154 13 lysis lysis NN cord-104231-fi8pskod 154 14 buffer buffer NN cord-104231-fi8pskod 154 15 containing contain VBG cord-104231-fi8pskod 154 16 0.1 0.1 CD cord-104231-fi8pskod 154 17 % % NN cord-104231-fi8pskod 154 18 SDS SDS NNP cord-104231-fi8pskod 154 19 , , , cord-104231-fi8pskod 154 20 once once RB cord-104231-fi8pskod 154 21 with with IN cord-104231-fi8pskod 154 22 lysis lysis NN cord-104231-fi8pskod 154 23 buffer buffer NN cord-104231-fi8pskod 154 24 containing contain VBG cord-104231-fi8pskod 154 25 0.5 0.5 CD cord-104231-fi8pskod 154 26 M M NNP cord-104231-fi8pskod 154 27 NaCI NaCI NNP cord-104231-fi8pskod 154 28 , , , cord-104231-fi8pskod 154 29 twice twice RB cord-104231-fi8pskod 154 30 with with IN cord-104231-fi8pskod 154 31 lysis lysis NN cord-104231-fi8pskod 154 32 buffer buffer NN cord-104231-fi8pskod 154 33 , , , cord-104231-fi8pskod 154 34 and and CC cord-104231-fi8pskod 154 35 finally finally RB cord-104231-fi8pskod 154 36 once once RB cord-104231-fi8pskod 154 37 with with IN cord-104231-fi8pskod 154 38 low low JJ cord-104231-fi8pskod 154 39 ionic ionic JJ cord-104231-fi8pskod 154 40 strength strength NN cord-104231-fi8pskod 154 41 buffer buffer NN cord-104231-fi8pskod 154 42 ( ( -LRB- cord-104231-fi8pskod 154 43 20 20 CD cord-104231-fi8pskod 154 44 rnM rnm JJ cord-104231-fi8pskod 154 45 Tris Tris NNP cord-104231-fi8pskod 154 46 pH pH NNP cord-104231-fi8pskod 154 47 7.5 7.5 CD cord-104231-fi8pskod 154 48 , , , cord-104231-fi8pskod 154 49 0.1 0.1 CD cord-104231-fi8pskod 154 50 % % NN cord-104231-fi8pskod 154 51 TX-100 TX-100 NNP cord-104231-fi8pskod 154 52 ) ) -RRB- cord-104231-fi8pskod 154 53 . . . cord-104231-fi8pskod 155 1 After after IN cord-104231-fi8pskod 155 2 the the DT cord-104231-fi8pskod 155 3 final final JJ cord-104231-fi8pskod 155 4 wash wash NN cord-104231-fi8pskod 155 5 , , , cord-104231-fi8pskod 155 6 immunoprecipitates immunoprecipitate NNS cord-104231-fi8pskod 155 7 were be VBD cord-104231-fi8pskod 155 8 divided divide VBN cord-104231-fi8pskod 155 9 equally equally RB cord-104231-fi8pskod 155 10 and and CC cord-104231-fi8pskod 155 11 resuspended resuspend VBD cord-104231-fi8pskod 155 12 in in IN cord-104231-fi8pskod 155 13 15 15 CD cord-104231-fi8pskod 155 14 # # $ cord-104231-fi8pskod 155 15 1 1 CD cord-104231-fi8pskod 155 16 of of IN cord-104231-fi8pskod 155 17 neuraminidase neuraminidase NN cord-104231-fi8pskod 155 18 digestion digestion NN cord-104231-fi8pskod 155 19 buffer buffer NN cord-104231-fi8pskod 155 20 ( ( -LRB- cord-104231-fi8pskod 155 21 300 300 CD cord-104231-fi8pskod 155 22 mM mm CD cord-104231-fi8pskod 155 23 NaC1 NaC1 NNPS cord-104231-fi8pskod 155 24 , , , cord-104231-fi8pskod 155 25 100 100 CD cord-104231-fi8pskod 155 26 ram ram NN cord-104231-fi8pskod 155 27 sodium sodium NN cord-104231-fi8pskod 155 28 acetate acetate NN cord-104231-fi8pskod 155 29 pH pH NNP cord-104231-fi8pskod 155 30 5.5 5.5 CD cord-104231-fi8pskod 155 31 , , , cord-104231-fi8pskod 155 32 14 14 CD cord-104231-fi8pskod 155 33 mM mm CD cord-104231-fi8pskod 155 34 calcium calcium NN cord-104231-fi8pskod 155 35 chloride chloride NN cord-104231-fi8pskod 155 36 and and CC cord-104231-fi8pskod 155 37 I i NN cord-104231-fi8pskod 155 38 mM mm CD cord-104231-fi8pskod 155 39 PMSF PMSF NNP cord-104231-fi8pskod 155 40 ) ) -RRB- cord-104231-fi8pskod 155 41 containing contain VBG cord-104231-fi8pskod 155 42 either either CC cord-104231-fi8pskod 155 43 25 25 CD cord-104231-fi8pskod 155 44 mU mU NNS cord-104231-fi8pskod 155 45 neuraminidase neuraminidase NN cord-104231-fi8pskod 155 46 ( ( -LRB- cord-104231-fi8pskod 155 47 Type type NN cord-104231-fi8pskod 155 48 VIII VIII NNP cord-104231-fi8pskod 155 49 ; ; : cord-104231-fi8pskod 155 50 Sigma Sigma NNP cord-104231-fi8pskod 155 51 ) ) -RRB- cord-104231-fi8pskod 155 52 or or CC cord-104231-fi8pskod 155 53 buffer buffer NN cord-104231-fi8pskod 155 54 ( ( -LRB- cord-104231-fi8pskod 155 55 mock mock JJ cord-104231-fi8pskod 155 56 treatment treatment NN cord-104231-fi8pskod 155 57 ) ) -RRB- cord-104231-fi8pskod 155 58 . . . cord-104231-fi8pskod 156 1 Samples sample NNS cord-104231-fi8pskod 156 2 were be VBD cord-104231-fi8pskod 156 3 incubated incubate VBN cord-104231-fi8pskod 156 4 at at IN cord-104231-fi8pskod 156 5 37"C 37"c CD cord-104231-fi8pskod 156 6 for for IN cord-104231-fi8pskod 156 7 4 4 CD cord-104231-fi8pskod 156 8 h h NN cord-104231-fi8pskod 156 9 before before IN cord-104231-fi8pskod 156 10 analysis analysis NN cord-104231-fi8pskod 156 11 by by IN cord-104231-fi8pskod 156 12 SDS SDS NNP cord-104231-fi8pskod 156 13 - - HYPH cord-104231-fi8pskod 156 14 PAGE PAGE NNP cord-104231-fi8pskod 156 15 and and CC cord-104231-fi8pskod 156 16 fluorography fluorography NN cord-104231-fi8pskod 156 17 . . . cord-104231-fi8pskod 157 1 We -PRON- PRP cord-104231-fi8pskod 157 2 have have VBP cord-104231-fi8pskod 157 3 used use VBN cord-104231-fi8pskod 157 4 both both DT cord-104231-fi8pskod 157 5 CD8 CD8 NNP cord-104231-fi8pskod 157 6 and and CC cord-104231-fi8pskod 157 7 CIM CIM NNP cord-104231-fi8pskod 157 8 as as IN cord-104231-fi8pskod 157 9 the the DT cord-104231-fi8pskod 157 10 reporter reporter NN cord-104231-fi8pskod 157 11 molecules molecule NNS cord-104231-fi8pskod 157 12 . . . cord-104231-fi8pskod 158 1 These these DT cord-104231-fi8pskod 158 2 glycoproteins glycoprotein NNS cord-104231-fi8pskod 158 3 are be VBP cord-104231-fi8pskod 158 4 members member NNS cord-104231-fi8pskod 158 5 of of IN cord-104231-fi8pskod 158 6 the the DT cord-104231-fi8pskod 158 7 Ig Ig NNP cord-104231-fi8pskod 158 8 superfamily superfamily NN cord-104231-fi8pskod 158 9 and and CC cord-104231-fi8pskod 158 10 are be VBP cord-104231-fi8pskod 158 11 normally normally RB cord-104231-fi8pskod 158 12 expressed express VBN cord-104231-fi8pskod 158 13 on on IN cord-104231-fi8pskod 158 14 the the DT cord-104231-fi8pskod 158 15 surface surface NN cord-104231-fi8pskod 158 16 of of IN cord-104231-fi8pskod 158 17 T t NN cord-104231-fi8pskod 158 18 - - HYPH cord-104231-fi8pskod 158 19 lymphocytes lymphocyte NNS cord-104231-fi8pskod 159 1 Maddon Maddon NNP cord-104231-fi8pskod 159 2 et et NNP cord-104231-fi8pskod 159 3 al al NNP cord-104231-fi8pskod 159 4 . . NNP cord-104231-fi8pskod 159 5 , , , cord-104231-fi8pskod 159 6 1985 1985 CD cord-104231-fi8pskod 159 7 ) ) -RRB- cord-104231-fi8pskod 159 8 . . . cord-104231-fi8pskod 160 1 The the DT cord-104231-fi8pskod 160 2 ~CD8 ~cd8 CD cord-104231-fi8pskod 160 3 polypeptide polypeptide NN cord-104231-fi8pskod 160 4 consists consist VBZ cord-104231-fi8pskod 160 5 of of IN cord-104231-fi8pskod 160 6 214 214 CD cord-104231-fi8pskod 160 7 amino amino JJ cord-104231-fi8pskod 160 8 acids acid NNS cord-104231-fi8pskod 161 1 ( ( -LRB- cord-104231-fi8pskod 161 2 Littman Littman NNP cord-104231-fi8pskod 161 3 et et NNP cord-104231-fi8pskod 161 4 ai ai NNP cord-104231-fi8pskod 161 5 . . NNP cord-104231-fi8pskod 161 6 , , , cord-104231-fi8pskod 161 7 1985 1985 CD cord-104231-fi8pskod 161 8 ) ) -RRB- cord-104231-fi8pskod 161 9 and and CC cord-104231-fi8pskod 161 10 CD4 CD4 NNP cord-104231-fi8pskod 161 11 of of IN cord-104231-fi8pskod 161 12 435 435 CD cord-104231-fi8pskod 161 13 residues residue NNS cord-104231-fi8pskod 161 14 ( ( -LRB- cord-104231-fi8pskod 161 15 Maddon Maddon NNP cord-104231-fi8pskod 161 16 et et NNP cord-104231-fi8pskod 161 17 al al NNP cord-104231-fi8pskod 161 18 . . NNP cord-104231-fi8pskod 161 19 , , , cord-104231-fi8pskod 161 20 1985 1985 CD cord-104231-fi8pskod 161 21 ) ) -RRB- cord-104231-fi8pskod 161 22 . . . cord-104231-fi8pskod 162 1 The the DT cord-104231-fi8pskod 162 2 amino amino NN cord-104231-fi8pskod 162 3 acid acid NN cord-104231-fi8pskod 162 4 sequences sequence NNS cord-104231-fi8pskod 162 5 of of IN cord-104231-fi8pskod 162 6 the the DT cord-104231-fi8pskod 162 7 membrane membrane NN cord-104231-fi8pskod 162 8 - - HYPH cord-104231-fi8pskod 162 9 spanning span VBG cord-104231-fi8pskod 162 10 and and CC cord-104231-fi8pskod 162 11 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 162 12 domains domain NNS cord-104231-fi8pskod 162 13 of of IN cord-104231-fi8pskod 162 14 CD8 CD8 NNP cord-104231-fi8pskod 162 15 and and CC cord-104231-fi8pskod 162 16 CD4 CD4 NNP cord-104231-fi8pskod 162 17 are be VBP cord-104231-fi8pskod 162 18 shown show VBN cord-104231-fi8pskod 162 19 in in IN cord-104231-fi8pskod 162 20 Fig Fig NNP cord-104231-fi8pskod 162 21 . . . cord-104231-fi8pskod 163 1 1 1 CD cord-104231-fi8pskod 163 2 A. a. NN cord-104231-fi8pskod 163 3 Rat Rat NNP cord-104231-fi8pskod 163 4 TGN38 TGN38 NNP cord-104231-fi8pskod 163 5 is be VBZ cord-104231-fi8pskod 163 6 a a DT cord-104231-fi8pskod 163 7 type type NN cord-104231-fi8pskod 163 8 I -PRON- PRP cord-104231-fi8pskod 163 9 glycoprotein glycoprotein VBP cord-104231-fi8pskod 163 10 with with IN cord-104231-fi8pskod 163 11 a a DT cord-104231-fi8pskod 163 12 predicted predict VBN cord-104231-fi8pskod 163 13 molecular molecular JJ cord-104231-fi8pskod 163 14 weight weight NN cord-104231-fi8pskod 163 15 of of IN cord-104231-fi8pskod 163 16 38 38 CD cord-104231-fi8pskod 163 17 kD kD NNS cord-104231-fi8pskod 163 18 but but CC cord-104231-fi8pskod 163 19 glycosylation glycosylation NN cord-104231-fi8pskod 163 20 increases increase VBZ cord-104231-fi8pskod 163 21 this this DT cord-104231-fi8pskod 163 22 to to IN cord-104231-fi8pskod 163 23 85 85 CD cord-104231-fi8pskod 163 24 - - SYM cord-104231-fi8pskod 163 25 95 95 CD cord-104231-fi8pskod 163 26 kD kd XX cord-104231-fi8pskod 164 1 ( ( -LRB- cord-104231-fi8pskod 164 2 Luzio Luzio NNP cord-104231-fi8pskod 164 3 et et FW cord-104231-fi8pskod 164 4 al al NNP cord-104231-fi8pskod 164 5 . . NNP cord-104231-fi8pskod 164 6 , , , cord-104231-fi8pskod 164 7 1990 1990 CD cord-104231-fi8pskod 164 8 ) ) -RRB- cord-104231-fi8pskod 164 9 . . . cord-104231-fi8pskod 165 1 Signal signal NN cord-104231-fi8pskod 165 2 peptide peptide JJ cord-104231-fi8pskod 165 3 cleavage cleavage NN cord-104231-fi8pskod 165 4 produces produce VBZ cord-104231-fi8pskod 165 5 a a DT cord-104231-fi8pskod 165 6 mature mature JJ cord-104231-fi8pskod 165 7 protein protein NN cord-104231-fi8pskod 165 8 of of IN cord-104231-fi8pskod 165 9 340 340 CD cord-104231-fi8pskod 165 10 residues residue NNS cord-104231-fi8pskod 165 11 . . . cord-104231-fi8pskod 166 1 The the DT cord-104231-fi8pskod 166 2 large large JJ cord-104231-fi8pskod 166 3 lumenal lumenal JJ cord-104231-fi8pskod 166 4 domain domain NN cord-104231-fi8pskod 166 5 ( ( -LRB- cord-104231-fi8pskod 166 6 residues residue NNS cord-104231-fi8pskod 166 7 1 1 CD cord-104231-fi8pskod 166 8 - - SYM cord-104231-fi8pskod 166 9 287 287 CD cord-104231-fi8pskod 166 10 ) ) -RRB- cord-104231-fi8pskod 166 11 is be VBZ cord-104231-fi8pskod 166 12 followed follow VBN cord-104231-fi8pskod 166 13 by by IN cord-104231-fi8pskod 166 14 a a DT cord-104231-fi8pskod 166 15 single single JJ cord-104231-fi8pskod 166 16 membrane membrane NN cord-104231-fi8pskod 166 17 - - HYPH cord-104231-fi8pskod 166 18 spanning span VBG cord-104231-fi8pskod 166 19 domain domain NN cord-104231-fi8pskod 166 20 ( ( -LRB- cord-104231-fi8pskod 166 21 residues residue NNS cord-104231-fi8pskod 166 22 288 288 CD cord-104231-fi8pskod 166 23 - - SYM cord-104231-fi8pskod 166 24 306 306 CD cord-104231-fi8pskod 166 25 ) ) -RRB- cord-104231-fi8pskod 166 26 and and CC cord-104231-fi8pskod 166 27 a a DT cord-104231-fi8pskod 166 28 short short JJ cord-104231-fi8pskod 166 29 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 166 30 domain domain NN cord-104231-fi8pskod 166 31 ( ( -LRB- cord-104231-fi8pskod 166 32 residues residue NNS cord-104231-fi8pskod 166 33 307 307 CD cord-104231-fi8pskod 166 34 - - SYM cord-104231-fi8pskod 166 35 340 340 CD cord-104231-fi8pskod 166 36 ; ; : cord-104231-fi8pskod 166 37 see see VB cord-104231-fi8pskod 166 38 Fig Fig NNP cord-104231-fi8pskod 166 39 . . . cord-104231-fi8pskod 167 1 I -PRON- PRP cord-104231-fi8pskod 167 2 A A NNP cord-104231-fi8pskod 167 3 ) ) -RRB- cord-104231-fi8pskod 167 4 . . . cord-104231-fi8pskod 168 1 Chimeric chimeric JJ cord-104231-fi8pskod 168 2 cDNAs cdna NNS cord-104231-fi8pskod 168 3 were be VBD cord-104231-fi8pskod 168 4 constructed construct VBN cord-104231-fi8pskod 168 5 by by IN cord-104231-fi8pskod 168 6 replacing replace VBG cord-104231-fi8pskod 168 7 domains domain NNS cord-104231-fi8pskod 168 8 in in IN cord-104231-fi8pskod 168 9 CD4 CD4 NNP cord-104231-fi8pskod 168 10 and and CC cord-104231-fi8pskod 168 11 CD8 CD8 NNP cord-104231-fi8pskod 168 12 with with IN cord-104231-fi8pskod 168 13 those those DT cord-104231-fi8pskod 168 14 in in IN cord-104231-fi8pskod 168 15 TGN38 TGN38 NNS cord-104231-fi8pskod 168 16 at at IN cord-104231-fi8pskod 168 17 identical identical JJ cord-104231-fi8pskod 168 18 amino amino JJ cord-104231-fi8pskod 168 19 acids acid NNS cord-104231-fi8pskod 168 20 in in IN cord-104231-fi8pskod 168 21 the the DT cord-104231-fi8pskod 168 22 three three CD cord-104231-fi8pskod 168 23 proteins protein NNS cord-104231-fi8pskod 168 24 ( ( -LRB- cord-104231-fi8pskod 168 25 see see VB cord-104231-fi8pskod 168 26 Fig Fig NNP cord-104231-fi8pskod 168 27 . . . cord-104231-fi8pskod 168 28 1 1 CD cord-104231-fi8pskod 168 29 A a CD cord-104231-fi8pskod 168 30 ) ) -RRB- cord-104231-fi8pskod 168 31 . . . cord-104231-fi8pskod 169 1 This this DT cord-104231-fi8pskod 169 2 meant mean VBD cord-104231-fi8pskod 169 3 that that IN cord-104231-fi8pskod 169 4 hybrid hybrid JJ cord-104231-fi8pskod 169 5 proteins protein NNS cord-104231-fi8pskod 169 6 containing contain VBG cord-104231-fi8pskod 169 7 the the DT cord-104231-fi8pskod 169 8 membrane membrane NN cord-104231-fi8pskod 169 9 - - HYPH cord-104231-fi8pskod 169 10 spanning span VBG cord-104231-fi8pskod 169 11 domain domain NN cord-104231-fi8pskod 169 12 of of IN cord-104231-fi8pskod 169 13 TGN38 TGN38 NNS cord-104231-fi8pskod 169 14 also also RB cord-104231-fi8pskod 169 15 included include VBD cord-104231-fi8pskod 169 16 some some DT cord-104231-fi8pskod 169 17 of of IN cord-104231-fi8pskod 169 18 the the DT cord-104231-fi8pskod 169 19 flanking flank VBG cord-104231-fi8pskod 169 20 amino amino NN cord-104231-fi8pskod 169 21 acids acid NNS cord-104231-fi8pskod 169 22 . . . cord-104231-fi8pskod 170 1 Flanking flanking NN cord-104231-fi8pskod 170 2 sequences sequence NNS cord-104231-fi8pskod 170 3 have have VBP cord-104231-fi8pskod 170 4 been be VBN cord-104231-fi8pskod 170 5 shown show VBN cord-104231-fi8pskod 170 6 to to TO cord-104231-fi8pskod 170 7 influence influence VB cord-104231-fi8pskod 170 8 substantiaily substantiaily NN cord-104231-fi8pskod 170 9 the the DT cord-104231-fi8pskod 170 10 ability ability NN cord-104231-fi8pskod 170 11 of of IN cord-104231-fi8pskod 170 12 membrane membrane NN cord-104231-fi8pskod 170 13 - - HYPH cord-104231-fi8pskod 170 14 spanning span VBG cord-104231-fi8pskod 170 15 domains domain NNS cord-104231-fi8pskod 170 16 to to TO cord-104231-fi8pskod 170 17 localize localize VB cord-104231-fi8pskod 170 18 proteins protein NNS cord-104231-fi8pskod 170 19 to to IN cord-104231-fi8pskod 170 20 the the DT cord-104231-fi8pskod 170 21 Golgi Golgi NNP cord-104231-fi8pskod 170 22 apparatus apparatus NN cord-104231-fi8pskod 170 23 ( ( -LRB- cord-104231-fi8pskod 171 1 Nilsson Nilsson NNP cord-104231-fi8pskod 171 2 et et NNP cord-104231-fi8pskod 171 3 al al NNP cord-104231-fi8pskod 171 4 . . . cord-104231-fi8pskod 172 1 , , , cord-104231-fi8pskod 172 2 1991 1991 CD cord-104231-fi8pskod 172 3 ; ; : cord-104231-fi8pskod 172 4 Munro Munro NNP cord-104231-fi8pskod 172 5 , , , cord-104231-fi8pskod 172 6 1991 1991 CD cord-104231-fi8pskod 172 7 ; ; : cord-104231-fi8pskod 172 8 Colley Colley NNP cord-104231-fi8pskod 172 9 et et NNP cord-104231-fi8pskod 172 10 al al NNP cord-104231-fi8pskod 172 11 . . NNP cord-104231-fi8pskod 172 12 , , , cord-104231-fi8pskod 172 13 1992 1992 CD cord-104231-fi8pskod 172 14 ) ) -RRB- cord-104231-fi8pskod 172 15 . . . cord-104231-fi8pskod 173 1 Rat rat NN cord-104231-fi8pskod 173 2 TGN38 TGN38 NNP cord-104231-fi8pskod 174 1 eDNA eDNA NNP cord-104231-fi8pskod 175 1 ( ( -LRB- cord-104231-fi8pskod 175 2 Luzio Luzio NNP cord-104231-fi8pskod 175 3 et et FW cord-104231-fi8pskod 175 4 al al NNP cord-104231-fi8pskod 175 5 . . NNP cord-104231-fi8pskod 175 6 , , , cord-104231-fi8pskod 175 7 1990 1990 CD cord-104231-fi8pskod 175 8 ) ) -RRB- cord-104231-fi8pskod 175 9 was be VBD cord-104231-fi8pskod 175 10 cloned clone VBN cord-104231-fi8pskod 175 11 into into IN cord-104231-fi8pskod 175 12 the the DT cord-104231-fi8pskod 175 13 pCMUIV pcmuiv NN cord-104231-fi8pskod 175 14 expression expression NN cord-104231-fi8pskod 175 15 vector vector NN cord-104231-fi8pskod 175 16 ( ( -LRB- cord-104231-fi8pskod 175 17 see see VB cord-104231-fi8pskod 175 18 Materials material NNS cord-104231-fi8pskod 175 19 and and CC cord-104231-fi8pskod 175 20 Methods Methods NNPS cord-104231-fi8pskod 175 21 ) ) -RRB- cord-104231-fi8pskod 175 22 and and CC cord-104231-fi8pskod 175 23 the the DT cord-104231-fi8pskod 175 24 plasmid plasmid NN cord-104231-fi8pskod 175 25 introduced introduce VBN cord-104231-fi8pskod 175 26 into into IN cord-104231-fi8pskod 175 27 cells cell NNS cord-104231-fi8pskod 175 28 by by IN cord-104231-fi8pskod 175 29 calcium calcium NN cord-104231-fi8pskod 175 30 phosphate phosphate NN cord-104231-fi8pskod 175 31 transfection transfection NN cord-104231-fi8pskod 175 32 . . . cord-104231-fi8pskod 176 1 At at IN cord-104231-fi8pskod 176 2 72 72 CD cord-104231-fi8pskod 176 3 h h NN cord-104231-fi8pskod 176 4 after after IN cord-104231-fi8pskod 176 5 transfection transfection NN cord-104231-fi8pskod 176 6 , , , cord-104231-fi8pskod 176 7 immunofluorescence immunofluorescence NN cord-104231-fi8pskod 176 8 microscopy microscopy NNS cord-104231-fi8pskod 176 9 showed show VBD cord-104231-fi8pskod 176 10 that that IN cord-104231-fi8pskod 176 11 TGN38 TGN38 NNP cord-104231-fi8pskod 176 12 was be VBD cord-104231-fi8pskod 176 13 localized localize VBN cord-104231-fi8pskod 176 14 to to IN cord-104231-fi8pskod 176 15 a a DT cord-104231-fi8pskod 176 16 juxtanuclear juxtanuclear NN cord-104231-fi8pskod 176 17 reticulum reticulum NN cord-104231-fi8pskod 176 18 ( ( -LRB- cord-104231-fi8pskod 176 19 Fig Fig NNP cord-104231-fi8pskod 176 20 . . . cord-104231-fi8pskod 176 21 2 2 CD cord-104231-fi8pskod 176 22 A a CD cord-104231-fi8pskod 176 23 ) ) -RRB- cord-104231-fi8pskod 176 24 very very RB cord-104231-fi8pskod 176 25 similar similar JJ cord-104231-fi8pskod 176 26 to to IN cord-104231-fi8pskod 176 27 that that DT cord-104231-fi8pskod 176 28 stained stain VBN cord-104231-fi8pskod 176 29 by by IN cord-104231-fi8pskod 176 30 antibodies antibody NNS cord-104231-fi8pskod 176 31 to to IN cord-104231-fi8pskod 176 32 GaIT GaIT NNP cord-104231-fi8pskod 177 1 ( ( -LRB- cord-104231-fi8pskod 177 2 Fig Fig NNP cord-104231-fi8pskod 177 3 . . NNP cord-104231-fi8pskod 177 4 2 2 CD cord-104231-fi8pskod 177 5 B b NN cord-104231-fi8pskod 177 6 ) ) -RRB- cord-104231-fi8pskod 177 7 , , , cord-104231-fi8pskod 177 8 a a DT cord-104231-fi8pskod 177 9 resident resident NN cord-104231-fi8pskod 177 10 of of IN cord-104231-fi8pskod 177 11 the the DT cord-104231-fi8pskod 177 12 trans trans NNP cord-104231-fi8pskod 177 13 - - NNP cord-104231-fi8pskod 177 14 Golgi Golgi NNP cord-104231-fi8pskod 177 15 cisterna cisterna NN cord-104231-fi8pskod 177 16 ( ( -LRB- cord-104231-fi8pskod 177 17 Roth Roth NNP cord-104231-fi8pskod 177 18 and and CC cord-104231-fi8pskod 177 19 Berger Berger NNP cord-104231-fi8pskod 177 20 , , , cord-104231-fi8pskod 177 21 1982 1982 CD cord-104231-fi8pskod 177 22 ) ) -RRB- cord-104231-fi8pskod 177 23 and and CC cord-104231-fi8pskod 177 24 the the DT cord-104231-fi8pskod 177 25 TGN TGN NNP cord-104231-fi8pskod 177 26 ( ( -LRB- cord-104231-fi8pskod 177 27 Lucocq Lucocq NNP cord-104231-fi8pskod 177 28 et et FW cord-104231-fi8pskod 177 29 al al NNP cord-104231-fi8pskod 177 30 . . NNP cord-104231-fi8pskod 177 31 , , , cord-104231-fi8pskod 177 32 1989 1989 CD cord-104231-fi8pskod 177 33 ; ; : cord-104231-fi8pskod 177 34 Taatjes Taatjes NNP cord-104231-fi8pskod 177 35 et et NNP cord-104231-fi8pskod 177 36 ai ai NNP cord-104231-fi8pskod 177 37 . . NNP cord-104231-fi8pskod 177 38 , , , cord-104231-fi8pskod 177 39 1992 1992 CD cord-104231-fi8pskod 177 40 ; ; : cord-104231-fi8pskod 177 41 Nilsson Nilsson NNP cord-104231-fi8pskod 177 42 et et NNP cord-104231-fi8pskod 177 43 al al NNP cord-104231-fi8pskod 177 44 . . NNP cord-104231-fi8pskod 177 45 , , , cord-104231-fi8pskod 177 46 1993a 1993a CD cord-104231-fi8pskod 177 47 ) ) -RRB- cord-104231-fi8pskod 177 48 . . . cord-104231-fi8pskod 178 1 Higher high JJR cord-104231-fi8pskod 178 2 levels level NNS cord-104231-fi8pskod 178 3 of of IN cord-104231-fi8pskod 178 4 TGN38 TGN38 NNS cord-104231-fi8pskod 178 5 led lead VBD cord-104231-fi8pskod 178 6 to to IN cord-104231-fi8pskod 178 7 an an DT cord-104231-fi8pskod 178 8 accumulation accumulation NN cord-104231-fi8pskod 178 9 of of IN cord-104231-fi8pskod 178 10 the the DT cord-104231-fi8pskod 178 11 protein protein NN cord-104231-fi8pskod 178 12 in in IN cord-104231-fi8pskod 178 13 punctate punctate NN cord-104231-fi8pskod 178 14 structures structure NNS cord-104231-fi8pskod 178 15 in in IN cord-104231-fi8pskod 178 16 the the DT cord-104231-fi8pskod 178 17 cytoplasm cytoplasm NN cord-104231-fi8pskod 178 18 ( ( -LRB- cord-104231-fi8pskod 178 19 Fig fig NN cord-104231-fi8pskod 178 20 . . . cord-104231-fi8pskod 178 21 2 2 CD cord-104231-fi8pskod 178 22 A a CD cord-104231-fi8pskod 178 23 ) ) -RRB- cord-104231-fi8pskod 178 24 and and CC cord-104231-fi8pskod 178 25 at at IN cord-104231-fi8pskod 178 26 the the DT cord-104231-fi8pskod 178 27 cell cell NN cord-104231-fi8pskod 178 28 surface surface NN cord-104231-fi8pskod 178 29 ( ( -LRB- cord-104231-fi8pskod 178 30 data datum NNS cord-104231-fi8pskod 178 31 not not RB cord-104231-fi8pskod 178 32 shown show VBN cord-104231-fi8pskod 178 33 ; ; : cord-104231-fi8pskod 178 34 see see VB cord-104231-fi8pskod 178 35 later later RB cord-104231-fi8pskod 178 36 ) ) -RRB- cord-104231-fi8pskod 178 37 . . . cord-104231-fi8pskod 179 1 These these DT cord-104231-fi8pskod 179 2 data datum NNS cord-104231-fi8pskod 179 3 are be VBP cord-104231-fi8pskod 179 4 consistent consistent JJ cord-104231-fi8pskod 179 5 with with IN cord-104231-fi8pskod 179 6 the the DT cord-104231-fi8pskod 179 7 localization localization NN cord-104231-fi8pskod 179 8 of of IN cord-104231-fi8pskod 179 9 endogenous endogenous JJ cord-104231-fi8pskod 179 10 TGN38 TGN38 NNS cord-104231-fi8pskod 179 11 in in IN cord-104231-fi8pskod 179 12 NRK NRK NNP cord-104231-fi8pskod 179 13 cells cell NNS cord-104231-fi8pskod 179 14 and and CC cord-104231-fi8pskod 179 15 expressed express VBD cord-104231-fi8pskod 179 16 protein protein NN cord-104231-fi8pskod 179 17 in in IN cord-104231-fi8pskod 179 18 transfected transfecte VBN cord-104231-fi8pskod 179 19 CV-1 CV-1 NNP cord-104231-fi8pskod 179 20 cells cell NNS cord-104231-fi8pskod 179 21 ( ( -LRB- cord-104231-fi8pskod 179 22 Luzio Luzio NNP cord-104231-fi8pskod 179 23 et et FW cord-104231-fi8pskod 179 24 al al NNP cord-104231-fi8pskod 179 25 . . NNP cord-104231-fi8pskod 179 26 , , , cord-104231-fi8pskod 179 27 1990 1990 CD cord-104231-fi8pskod 179 28 ) ) -RRB- cord-104231-fi8pskod 179 29 . . . cord-104231-fi8pskod 180 1 The the DT cord-104231-fi8pskod 180 2 membrane membrane NN cord-104231-fi8pskod 180 3 - - HYPH cord-104231-fi8pskod 180 4 spanning span VBG cord-104231-fi8pskod 180 5 ( ( -LRB- cord-104231-fi8pskod 180 6 M M NNP cord-104231-fi8pskod 180 7 ) ) -RRB- cord-104231-fi8pskod 180 8 and and CC cord-104231-fi8pskod 180 9 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 180 10 ( ( -LRB- cord-104231-fi8pskod 180 11 C C NNP cord-104231-fi8pskod 180 12 ) ) -RRB- cord-104231-fi8pskod 180 13 domains domain NNS cord-104231-fi8pskod 180 14 of of IN cord-104231-fi8pskod 180 15 TGN38 TGN38 NNS cord-104231-fi8pskod 180 16 were be VBD cord-104231-fi8pskod 180 17 grafted graft VBN cord-104231-fi8pskod 180 18 on on IN cord-104231-fi8pskod 180 19 to to IN cord-104231-fi8pskod 180 20 the the DT cord-104231-fi8pskod 180 21 lumenal lumenal JJ cord-104231-fi8pskod 180 22 domain domain NN cord-104231-fi8pskod 180 23 of of IN cord-104231-fi8pskod 180 24 CD8 CD8 NNP cord-104231-fi8pskod 180 25 ( ( -LRB- cord-104231-fi8pskod 180 26 see see VB cord-104231-fi8pskod 180 27 Fig Fig NNP cord-104231-fi8pskod 180 28 . . . cord-104231-fi8pskod 180 29 1 1 CD cord-104231-fi8pskod 180 30 B b NN cord-104231-fi8pskod 180 31 ) ) -RRB- cord-104231-fi8pskod 180 32 . . . cord-104231-fi8pskod 181 1 CD8 CD8 NNP cord-104231-fi8pskod 181 2 was be VBD cord-104231-fi8pskod 181 3 mostly mostly RB cord-104231-fi8pskod 181 4 present present JJ cord-104231-fi8pskod 181 5 on on IN cord-104231-fi8pskod 181 6 the the DT cord-104231-fi8pskod 181 7 cell cell NN cord-104231-fi8pskod 181 8 surface surface NN cord-104231-fi8pskod 181 9 though though IN cord-104231-fi8pskod 181 10 a a DT cord-104231-fi8pskod 181 11 small small JJ cord-104231-fi8pskod 181 12 amount amount NN cord-104231-fi8pskod 181 13 was be VBD cord-104231-fi8pskod 181 14 present present JJ cord-104231-fi8pskod 181 15 in in IN cord-104231-fi8pskod 181 16 small small JJ cord-104231-fi8pskod 181 17 punctate punctate NN cord-104231-fi8pskod 181 18 structures structure NNS cord-104231-fi8pskod 181 19 , , , cord-104231-fi8pskod 181 20 especially especially RB cord-104231-fi8pskod 181 21 at at IN cord-104231-fi8pskod 181 22 high high JJ cord-104231-fi8pskod 181 23 levels level NNS cord-104231-fi8pskod 181 24 of of IN cord-104231-fi8pskod 181 25 expression expression NN cord-104231-fi8pskod 182 1 ( ( -LRB- cord-104231-fi8pskod 182 2 Fig Fig NNP cord-104231-fi8pskod 182 3 . . NNP cord-104231-fi8pskod 182 4 2 2 CD cord-104231-fi8pskod 182 5 C C NNP cord-104231-fi8pskod 182 6 ) ) -RRB- cord-104231-fi8pskod 182 7 . . . cord-104231-fi8pskod 183 1 The the DT cord-104231-fi8pskod 183 2 hybrid hybrid JJ cord-104231-fi8pskod 183 3 protein protein NN cord-104231-fi8pskod 183 4 ( ( -LRB- cord-104231-fi8pskod 183 5 CD8-MC CD8-MC NNP cord-104231-fi8pskod 183 6 ) ) -RRB- cord-104231-fi8pskod 183 7 , , , cord-104231-fi8pskod 183 8 however however RB cord-104231-fi8pskod 183 9 , , , cord-104231-fi8pskod 183 10 was be VBD cord-104231-fi8pskod 183 11 restricted restrict VBN cord-104231-fi8pskod 183 12 to to IN cord-104231-fi8pskod 183 13 a a DT cord-104231-fi8pskod 183 14 compact compact JJ cord-104231-fi8pskod 183 15 juxtanuclear juxtanuclear NN cord-104231-fi8pskod 183 16 reticulum reticulum NN cord-104231-fi8pskod 183 17 ( ( -LRB- cord-104231-fi8pskod 183 18 Fig Fig NNP cord-104231-fi8pskod 183 19 . . . cord-104231-fi8pskod 183 20 2 2 CD cord-104231-fi8pskod 183 21 D d NN cord-104231-fi8pskod 183 22 ) ) -RRB- cord-104231-fi8pskod 183 23 that that WDT cord-104231-fi8pskod 183 24 was be VBD cord-104231-fi8pskod 183 25 very very RB cord-104231-fi8pskod 183 26 similar similar JJ cord-104231-fi8pskod 183 27 to to IN cord-104231-fi8pskod 183 28 that that DT cord-104231-fi8pskod 183 29 stained stain VBN cord-104231-fi8pskod 183 30 by by IN cord-104231-fi8pskod 183 31 antibodies antibody NNS cord-104231-fi8pskod 183 32 to to IN cord-104231-fi8pskod 183 33 GaIT GaIT NNP cord-104231-fi8pskod 184 1 ( ( -LRB- cord-104231-fi8pskod 184 2 data datum NNS cord-104231-fi8pskod 184 3 not not RB cord-104231-fi8pskod 184 4 shown show VBN cord-104231-fi8pskod 184 5 ) ) -RRB- cord-104231-fi8pskod 184 6 . . . cord-104231-fi8pskod 185 1 The the DT cord-104231-fi8pskod 185 2 membrane membrane NN cord-104231-fi8pskod 185 3 - - HYPH cord-104231-fi8pskod 185 4 spanning span VBG cord-104231-fi8pskod 185 5 and and CC cord-104231-fi8pskod 185 6 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 185 7 domains domain NNS cord-104231-fi8pskod 185 8 of of IN cord-104231-fi8pskod 185 9 TGN38 TGN38 NNS cord-104231-fi8pskod 185 10 were be VBD cord-104231-fi8pskod 185 11 also also RB cord-104231-fi8pskod 185 12 grafted graft VBN cord-104231-fi8pskod 185 13 on on RP cord-104231-fi8pskod 185 14 to to IN cord-104231-fi8pskod 185 15 the the DT cord-104231-fi8pskod 185 16 lumenal lumenal JJ cord-104231-fi8pskod 185 17 domain domain NN cord-104231-fi8pskod 185 18 of of IN cord-104231-fi8pskod 185 19 CD4 CD4 NNP cord-104231-fi8pskod 185 20 . . . cord-104231-fi8pskod 186 1 Transiently Transiently NNP cord-104231-fi8pskod 186 2 expressed express VBD cord-104231-fi8pskod 186 3 CD4 CD4 NNP cord-104231-fi8pskod 186 4 was be VBD cord-104231-fi8pskod 186 5 mostly mostly RB cord-104231-fi8pskod 186 6 localized localize VBN cord-104231-fi8pskod 186 7 to to IN cord-104231-fi8pskod 186 8 the the DT cord-104231-fi8pskod 186 9 plasma plasma NN cord-104231-fi8pskod 186 10 membrane membrane NN cord-104231-fi8pskod 186 11 ( ( -LRB- cord-104231-fi8pskod 186 12 Fig fig NN cord-104231-fi8pskod 186 13 . . . cord-104231-fi8pskod 186 14 2 2 CD cord-104231-fi8pskod 186 15 E e NN cord-104231-fi8pskod 186 16 ) ) -RRB- cord-104231-fi8pskod 186 17 , , , cord-104231-fi8pskod 186 18 but but CC cord-104231-fi8pskod 186 19 the the DT cord-104231-fi8pskod 186 20 CD4-MC cd4-mc NN cord-104231-fi8pskod 186 21 hybrid hybrid NN cord-104231-fi8pskod 186 22 protein protein NN cord-104231-fi8pskod 186 23 was be VBD cord-104231-fi8pskod 186 24 again again RB cord-104231-fi8pskod 186 25 localized localize VBN cord-104231-fi8pskod 186 26 to to IN cord-104231-fi8pskod 186 27 a a DT cord-104231-fi8pskod 186 28 juxtanuclear juxtanuclear NNP cord-104231-fi8pskod 186 29 compartment compartment NN cord-104231-fi8pskod 186 30 ( ( -LRB- cord-104231-fi8pskod 186 31 Fig Fig NNP cord-104231-fi8pskod 186 32 . . . cord-104231-fi8pskod 186 33 _SP cord-104231-fi8pskod 186 34 2 2 CD cord-104231-fi8pskod 186 35 F F NNP cord-104231-fi8pskod 186 36 ) ) -RRB- cord-104231-fi8pskod 186 37 , , , cord-104231-fi8pskod 186 38 very very RB cord-104231-fi8pskod 186 39 similar similar JJ cord-104231-fi8pskod 186 40 to to IN cord-104231-fi8pskod 186 41 that that DT cord-104231-fi8pskod 186 42 stained stain VBN cord-104231-fi8pskod 186 43 by by IN cord-104231-fi8pskod 186 44 antibodies antibody NNS cord-104231-fi8pskod 186 45 to to IN cord-104231-fi8pskod 186 46 GaIT GaIT NNP cord-104231-fi8pskod 187 1 ( ( -LRB- cord-104231-fi8pskod 187 2 data datum NNS cord-104231-fi8pskod 187 3 not not RB cord-104231-fi8pskod 187 4 shown show VBN cord-104231-fi8pskod 187 5 ) ) -RRB- cord-104231-fi8pskod 187 6 . . . cord-104231-fi8pskod 188 1 There there EX cord-104231-fi8pskod 188 2 was be VBD cord-104231-fi8pskod 188 3 also also RB cord-104231-fi8pskod 188 4 faint faint JJ cord-104231-fi8pskod 188 5 staining staining NN cord-104231-fi8pskod 188 6 of of IN cord-104231-fi8pskod 188 7 the the DT cord-104231-fi8pskod 188 8 nuclear nuclear JJ cord-104231-fi8pskod 188 9 envelope envelope NN cord-104231-fi8pskod 188 10 , , , cord-104231-fi8pskod 188 11 suggesting suggest VBG cord-104231-fi8pskod 188 12 some some DT cord-104231-fi8pskod 188 13 accumulation accumulation NN cord-104231-fi8pskod 188 14 of of IN cord-104231-fi8pskod 188 15 both both DT cord-104231-fi8pskod 188 16 hybrid hybrid JJ cord-104231-fi8pskod 188 17 proteins protein NNS cord-104231-fi8pskod 188 18 within within IN cord-104231-fi8pskod 188 19 the the DT cord-104231-fi8pskod 188 20 ER ER NNP cord-104231-fi8pskod 188 21 ( ( -LRB- cord-104231-fi8pskod 188 22 arrows arrow NNS cord-104231-fi8pskod 188 23 in in IN cord-104231-fi8pskod 188 24 Fig Fig NNP cord-104231-fi8pskod 188 25 . . NNP cord-104231-fi8pskod 188 26 2 2 CD cord-104231-fi8pskod 188 27 , , , cord-104231-fi8pskod 188 28 D D NNP cord-104231-fi8pskod 188 29 and and CC cord-104231-fi8pskod 188 30 F F NNP cord-104231-fi8pskod 188 31 ) ) -RRB- cord-104231-fi8pskod 188 32 . . . cord-104231-fi8pskod 189 1 To to TO cord-104231-fi8pskod 189 2 show show VB cord-104231-fi8pskod 189 3 that that IN cord-104231-fi8pskod 189 4 the the DT cord-104231-fi8pskod 189 5 membrane membrane NN cord-104231-fi8pskod 189 6 - - HYPH cord-104231-fi8pskod 189 7 spanning span VBG cord-104231-fi8pskod 189 8 and and CC cord-104231-fi8pskod 189 9 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 189 10 domains domain NNS cord-104231-fi8pskod 189 11 of of IN cord-104231-fi8pskod 189 12 TGN38 TGN38 NNS cord-104231-fi8pskod 189 13 contained contain VBD cord-104231-fi8pskod 189 14 distinct distinct JJ cord-104231-fi8pskod 189 15 localization localization NN cord-104231-fi8pskod 189 16 signals signal NNS cord-104231-fi8pskod 189 17 , , , cord-104231-fi8pskod 189 18 each each DT cord-104231-fi8pskod 189 19 of of IN cord-104231-fi8pskod 189 20 these these DT cord-104231-fi8pskod 189 21 domains domain NNS cord-104231-fi8pskod 189 22 in in IN cord-104231-fi8pskod 189 23 both both CC cord-104231-fi8pskod 189 24 CIM CIM NNP cord-104231-fi8pskod 189 25 and and CC cord-104231-fi8pskod 189 26 CD8 CD8 NNP cord-104231-fi8pskod 189 27 was be VBD cord-104231-fi8pskod 189 28 replaced replace VBN cord-104231-fi8pskod 189 29 by by IN cord-104231-fi8pskod 189 30 that that DT cord-104231-fi8pskod 189 31 domains domain NNS cord-104231-fi8pskod 189 32 of of IN cord-104231-fi8pskod 189 33 CD8 CD8 NNP cord-104231-fi8pskod 189 34 , , , cord-104231-fi8pskod 189 35 CD4 CD4 NNP cord-104231-fi8pskod 189 36 , , , cord-104231-fi8pskod 189 37 and and CC cord-104231-fi8pskod 189 38 TGN38 TGN38 NNS cord-104231-fi8pskod 189 39 . . . cord-104231-fi8pskod 190 1 The the DT cord-104231-fi8pskod 190 2 membrane membrane NN cord-104231-fi8pskod 190 3 - - HYPH cord-104231-fi8pskod 190 4 spanning span VBG cord-104231-fi8pskod 190 5 domain domain NN cord-104231-fi8pskod 190 6 of of IN cord-104231-fi8pskod 190 7 CIM CIM NNP cord-104231-fi8pskod 190 8 is be VBZ cord-104231-fi8pskod 190 9 that that DT cord-104231-fi8pskod 190 10 defined define VBN cord-104231-fi8pskod 190 11 by by IN cord-104231-fi8pskod 190 12 Maddon Maddon NNP cord-104231-fi8pskod 190 13 et et NNP cord-104231-fi8pskod 190 14 al al NNP cord-104231-fi8pskod 190 15 . . . cord-104231-fi8pskod 191 1 ( ( -LRB- cord-104231-fi8pskod 191 2 1985 1985 CD cord-104231-fi8pskod 191 3 ) ) -RRB- cord-104231-fi8pskod 191 4 although although IN cord-104231-fi8pskod 191 5 it -PRON- PRP cord-104231-fi8pskod 191 6 could could MD cord-104231-fi8pskod 191 7 inelude inelude VB cord-104231-fi8pskod 191 8 an an DT cord-104231-fi8pskod 191 9 additional additional JJ cord-104231-fi8pskod 191 10 two two CD cord-104231-fi8pskod 191 11 amino amino NN cord-104231-fi8pskod 191 12 acids acid NNS cord-104231-fi8pskod 191 13 ( ( -LRB- cord-104231-fi8pskod 191 14 -CV- -CV- NNP cord-104231-fi8pskod 191 15 ) ) -RRB- cord-104231-fi8pskod 191 16 on on IN cord-104231-fi8pskod 191 17 the the DT cord-104231-fi8pskod 191 18 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 191 19 side side NN cord-104231-fi8pskod 191 20 of of IN cord-104231-fi8pskod 191 21 the the DT cord-104231-fi8pskod 191 22 membrane membrane NN cord-104231-fi8pskod 191 23 . . . cord-104231-fi8pskod 192 1 Amino amino NN cord-104231-fi8pskod 192 2 acid acid NN cord-104231-fi8pskod 192 3 residues residue NNS cord-104231-fi8pskod 192 4 at at IN cord-104231-fi8pskod 192 5 which which WDT cord-104231-fi8pskod 192 6 domains domain NNS cord-104231-fi8pskod 192 7 were be VBD cord-104231-fi8pskod 192 8 replaced replace VBN cord-104231-fi8pskod 192 9 in in IN cord-104231-fi8pskod 192 10 the the DT cord-104231-fi8pskod 192 11 different different JJ cord-104231-fi8pskod 192 12 hybrid hybrid NN cord-104231-fi8pskod 192 13 proteins protein NNS cord-104231-fi8pskod 192 14 are be VBP cord-104231-fi8pskod 192 15 numbered number VBN cord-104231-fi8pskod 192 16 . . . cord-104231-fi8pskod 193 1 ( ( -LRB- cord-104231-fi8pskod 193 2 B b NN cord-104231-fi8pskod 193 3 and and CC cord-104231-fi8pskod 193 4 C C NNP cord-104231-fi8pskod 193 5 ) ) -RRB- cord-104231-fi8pskod 194 1 Line line NN cord-104231-fi8pskod 194 2 sketches sketch NNS cord-104231-fi8pskod 194 3 show show VBP cord-104231-fi8pskod 194 4 CDS CDS NNP cord-104231-fi8pskod 194 5 , , , cord-104231-fi8pskod 194 6 CIM CIM NNP cord-104231-fi8pskod 194 7 , , , cord-104231-fi8pskod 194 8 and and CC cord-104231-fi8pskod 194 9 hybrid hybrid NN cord-104231-fi8pskod 194 10 proteins protein NNS cord-104231-fi8pskod 194 11 containing contain VBG cord-104231-fi8pskod 194 12 the the DT cord-104231-fi8pskod 194 13 membrane membrane NN cord-104231-fi8pskod 194 14 - - HYPH cord-104231-fi8pskod 194 15 spanning span VBG cord-104231-fi8pskod 194 16 and and CC cord-104231-fi8pskod 194 17 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 194 18 domains domain NNS cord-104231-fi8pskod 194 19 of of IN cord-104231-fi8pskod 194 20 TGN38 TGN38 NNS cord-104231-fi8pskod 194 21 ( ( -LRB- cord-104231-fi8pskod 194 22 shaded shaded JJ cord-104231-fi8pskod 194 23 and and CC cord-104231-fi8pskod 194 24 boxed box VBN cord-104231-fi8pskod 194 25 ) ) -RRB- cord-104231-fi8pskod 194 26 . . . cord-104231-fi8pskod 195 1 Hybrid hybrid NN cord-104231-fi8pskod 195 2 proteins protein NNS cord-104231-fi8pskod 195 3 are be VBP cord-104231-fi8pskod 195 4 defined define VBN cord-104231-fi8pskod 195 5 by by IN cord-104231-fi8pskod 195 6 suffixes suffix NNS cord-104231-fi8pskod 195 7 which which WDT cord-104231-fi8pskod 195 8 denote denote VBP cord-104231-fi8pskod 195 9 the the DT cord-104231-fi8pskod 195 10 domains domain NNS cord-104231-fi8pskod 195 11 of of IN cord-104231-fi8pskod 195 12 CD8 CD8 NNP cord-104231-fi8pskod 195 13 or or CC cord-104231-fi8pskod 195 14 CD4 CD4 NNP cord-104231-fi8pskod 195 15 that that WDT cord-104231-fi8pskod 195 16 were be VBD cord-104231-fi8pskod 195 17 replaced replace VBN cord-104231-fi8pskod 195 18 by by IN cord-104231-fi8pskod 195 19 equivalent equivalent JJ cord-104231-fi8pskod 195 20 domains domain NNS cord-104231-fi8pskod 195 21 of of IN cord-104231-fi8pskod 195 22 TGN38 TGN38 NNS cord-104231-fi8pskod 195 23 . . . cord-104231-fi8pskod 196 1 M M NNP cord-104231-fi8pskod 196 2 = = SYM cord-104231-fi8pskod 196 3 membranespanning membranespanne VBG cord-104231-fi8pskod 196 4 domain domain NN cord-104231-fi8pskod 196 5 and and CC cord-104231-fi8pskod 196 6 flanking flanking JJ cord-104231-fi8pskod 196 7 residues residue NNS cord-104231-fi8pskod 196 8 ; ; : cord-104231-fi8pskod 196 9 C c NN cord-104231-fi8pskod 196 10 = = SYM cord-104231-fi8pskod 196 11 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 196 12 domain domain NN cord-104231-fi8pskod 196 13 ; ; : cord-104231-fi8pskod 196 14 and and CC cord-104231-fi8pskod 197 1 AC ac NN cord-104231-fi8pskod 197 2 = = SYM cord-104231-fi8pskod 197 3 truncated truncate VBD cord-104231-fi8pskod 197 4 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 197 5 domain domain NN cord-104231-fi8pskod 197 6 lacking lack VBG cord-104231-fi8pskod 197 7 21 21 CD cord-104231-fi8pskod 197 8 residues residue NNS cord-104231-fi8pskod 197 9 from from IN cord-104231-fi8pskod 197 10 the the DT cord-104231-fi8pskod 197 11 carboxyterminus carboxyterminus NNP cord-104231-fi8pskod 197 12 . . . cord-104231-fi8pskod 198 1 in in IN cord-104231-fi8pskod 198 2 TGN38 tgn38 ADD cord-104231-fi8pskod 199 1 ( ( -LRB- cord-104231-fi8pskod 199 2 Fig Fig NNP cord-104231-fi8pskod 199 3 . . NNP cord-104231-fi8pskod 199 4 1 1 CD cord-104231-fi8pskod 199 5 , , , cord-104231-fi8pskod 199 6 B b NN cord-104231-fi8pskod 199 7 and and CC cord-104231-fi8pskod 199 8 C C NNP cord-104231-fi8pskod 199 9 ) ) -RRB- cord-104231-fi8pskod 199 10 . . . cord-104231-fi8pskod 200 1 The the DT cord-104231-fi8pskod 200 2 TGN38 tgn38 CD cord-104231-fi8pskod 200 3 membranespanning membranespanne VBG cord-104231-fi8pskod 200 4 domain domain NN cord-104231-fi8pskod 200 5 alone alone RB cord-104231-fi8pskod 200 6 localized localize VBD cord-104231-fi8pskod 200 7 the the DT cord-104231-fi8pskod 200 8 hybrid hybrid JJ cord-104231-fi8pskod 200 9 proteins protein NNS cord-104231-fi8pskod 200 10 to to IN cord-104231-fi8pskod 200 11 the the DT cord-104231-fi8pskod 200 12 Golgi Golgi NNP cord-104231-fi8pskod 200 13 apparatus apparatus NN cord-104231-fi8pskod 200 14 . . . cord-104231-fi8pskod 201 1 Both both DT cord-104231-fi8pskod 201 2 CD8-M CD8-M NNPS cord-104231-fi8pskod 202 1 ( ( -LRB- cord-104231-fi8pskod 202 2 Fig Fig NNP cord-104231-fi8pskod 202 3 . . . cord-104231-fi8pskod 202 4 3 3 CD cord-104231-fi8pskod 202 5 A a NN cord-104231-fi8pskod 202 6 ) ) -RRB- cord-104231-fi8pskod 203 1 and and CC cord-104231-fi8pskod 203 2 CD4-M CD4-M NNP cord-104231-fi8pskod 203 3 ( ( -LRB- cord-104231-fi8pskod 203 4 Fig Fig NNP cord-104231-fi8pskod 203 5 . . . cord-104231-fi8pskod 203 6 _SP cord-104231-fi8pskod 203 7 3 3 CD cord-104231-fi8pskod 203 8 E E NNP cord-104231-fi8pskod 203 9 ) ) -RRB- cord-104231-fi8pskod 203 10 gave give VBD cord-104231-fi8pskod 203 11 a a DT cord-104231-fi8pskod 203 12 discrete discrete JJ cord-104231-fi8pskod 203 13 juxtanuclear juxtanuclear NN cord-104231-fi8pskod 203 14 staining staining NN cord-104231-fi8pskod 203 15 pattern pattern NN cord-104231-fi8pskod 203 16 very very RB cord-104231-fi8pskod 203 17 similar similar JJ cord-104231-fi8pskod 203 18 to to IN cord-104231-fi8pskod 203 19 that that DT cord-104231-fi8pskod 203 20 for for IN cord-104231-fi8pskod 203 21 GAIT GAIT NNP cord-104231-fi8pskod 203 22 , , , cord-104231-fi8pskod 203 23 especially especially RB cord-104231-fi8pskod 203 24 at at IN cord-104231-fi8pskod 203 25 low low JJ cord-104231-fi8pskod 203 26 levels level NNS cord-104231-fi8pskod 203 27 of of IN cord-104231-fi8pskod 203 28 expression expression NN cord-104231-fi8pskod 204 1 ( ( -LRB- cord-104231-fi8pskod 204 2 Fig Fig NNP cord-104231-fi8pskod 204 3 . . . cord-104231-fi8pskod 204 4 3 3 CD cord-104231-fi8pskod 204 5 , , , cord-104231-fi8pskod 204 6 B B NNP cord-104231-fi8pskod 204 7 and and CC cord-104231-fi8pskod 204 8 F F NNP cord-104231-fi8pskod 204 9 , , , cord-104231-fi8pskod 204 10 , , , cord-104231-fi8pskod 204 11 respectively respectively RB cord-104231-fi8pskod 204 12 ) ) -RRB- cord-104231-fi8pskod 204 13 . . . cord-104231-fi8pskod 205 1 At at IN cord-104231-fi8pskod 205 2 higher high JJR cord-104231-fi8pskod 205 3 levels level NNS cord-104231-fi8pskod 205 4 of of IN cord-104231-fi8pskod 205 5 expression expression NN cord-104231-fi8pskod 205 6 , , , cord-104231-fi8pskod 205 7 staining staining NN cord-104231-fi8pskod 205 8 was be VBD cord-104231-fi8pskod 205 9 more more RBR cord-104231-fi8pskod 205 10 diffuse diffuse JJ cord-104231-fi8pskod 205 11 suggesting suggest VBG cord-104231-fi8pskod 205 12 accumulation accumulation NN cord-104231-fi8pskod 205 13 in in IN cord-104231-fi8pskod 205 14 vesicles vesicle NNS cord-104231-fi8pskod 205 15 and and CC cord-104231-fi8pskod 205 16 at at IN cord-104231-fi8pskod 205 17 the the DT cord-104231-fi8pskod 205 18 cell cell NN cord-104231-fi8pskod 205 19 surface surface NN cord-104231-fi8pskod 205 20 ( ( -LRB- cord-104231-fi8pskod 205 21 data datum NNS cord-104231-fi8pskod 205 22 not not RB cord-104231-fi8pskod 205 23 shown show VBN cord-104231-fi8pskod 205 24 ) ) -RRB- cord-104231-fi8pskod 205 25 . . . cord-104231-fi8pskod 206 1 This this DT cord-104231-fi8pskod 206 2 pattern pattern NN cord-104231-fi8pskod 206 3 was be VBD cord-104231-fi8pskod 206 4 more more RBR cord-104231-fi8pskod 206 5 frequently frequently RB cord-104231-fi8pskod 206 6 observed observe VBN cord-104231-fi8pskod 206 7 for for IN cord-104231-fi8pskod 206 8 CD4-M CD4-M NNP cord-104231-fi8pskod 206 9 than than IN cord-104231-fi8pskod 206 10 for for IN cord-104231-fi8pskod 206 11 CDS CDS NNP cord-104231-fi8pskod 206 12 - - HYPH cord-104231-fi8pskod 206 13 M. M. NNP cord-104231-fi8pskod 207 1 The the DT cord-104231-fi8pskod 207 2 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 207 3 domain domain NN cord-104231-fi8pskod 207 4 of of IN cord-104231-fi8pskod 207 5 TGN38 TGN38 NNS cord-104231-fi8pskod 207 6 contained contain VBN cord-104231-fi8pskod 207 7 , , , cord-104231-fi8pskod 207 8 as as IN cord-104231-fi8pskod 207 9 expected expect VBN cord-104231-fi8pskod 207 10 , , , cord-104231-fi8pskod 207 11 the the DT cord-104231-fi8pskod 207 12 other other JJ cord-104231-fi8pskod 207 13 Golgi Golgi NNP cord-104231-fi8pskod 207 14 localization localization NN cord-104231-fi8pskod 207 15 signal signal NN cord-104231-fi8pskod 207 16 ( ( -LRB- cord-104231-fi8pskod 207 17 Bos Bos NNP cord-104231-fi8pskod 207 18 et et NNP cord-104231-fi8pskod 207 19 al al NNP cord-104231-fi8pskod 207 20 . . NNP cord-104231-fi8pskod 207 21 , , , cord-104231-fi8pskod 207 22 1993 1993 CD cord-104231-fi8pskod 207 23 ; ; : cord-104231-fi8pskod 207 24 Humphrey Humphrey NNP cord-104231-fi8pskod 207 25 et et NNP cord-104231-fi8pskod 207 26 al al NNP cord-104231-fi8pskod 207 27 . . NNP cord-104231-fi8pskod 207 28 , , , cord-104231-fi8pskod 207 29 1993 1993 CD cord-104231-fi8pskod 207 30 ; ; : cord-104231-fi8pskod 207 31 Wong Wong NNP cord-104231-fi8pskod 207 32 and and CC cord-104231-fi8pskod 207 33 Hong Hong NNP cord-104231-fi8pskod 207 34 , , , cord-104231-fi8pskod 207 35 1993 1993 CD cord-104231-fi8pskod 207 36 ) ) -RRB- cord-104231-fi8pskod 207 37 . . . cord-104231-fi8pskod 208 1 Both both DT cord-104231-fi8pskod 208 2 CD8-C CD8-C NNP cord-104231-fi8pskod 208 3 ( ( -LRB- cord-104231-fi8pskod 208 4 Fig fig NN cord-104231-fi8pskod 208 5 . . . cord-104231-fi8pskod 208 6 3 3 CD cord-104231-fi8pskod 208 7 C C NNP cord-104231-fi8pskod 208 8 ) ) -RRB- cord-104231-fi8pskod 208 9 and and CC cord-104231-fi8pskod 208 10 CIM CIM NNP cord-104231-fi8pskod 208 11 - - HYPH cord-104231-fi8pskod 208 12 C C NNP cord-104231-fi8pskod 209 1 ( ( -LRB- cord-104231-fi8pskod 209 2 Fig Fig NNP cord-104231-fi8pskod 209 3 . . . cord-104231-fi8pskod 209 4 3 3 CD cord-104231-fi8pskod 209 5 G g NN cord-104231-fi8pskod 209 6 ) ) -RRB- cord-104231-fi8pskod 209 7 were be VBD cord-104231-fi8pskod 209 8 detected detect VBN cord-104231-fi8pskod 209 9 in in IN cord-104231-fi8pskod 209 10 a a DT cord-104231-fi8pskod 209 11 juxtanuelear juxtanuelear NN cord-104231-fi8pskod 209 12 reticulum reticulum NN cord-104231-fi8pskod 209 13 very very RB cord-104231-fi8pskod 209 14 similar similar JJ cord-104231-fi8pskod 209 15 to to IN cord-104231-fi8pskod 209 16 that that DT cord-104231-fi8pskod 209 17 containing contain VBG cord-104231-fi8pskod 209 18 GaIT GaIT NNP cord-104231-fi8pskod 210 1 ( ( -LRB- cord-104231-fi8pskod 210 2 Fig Fig NNP cord-104231-fi8pskod 210 3 . . . cord-104231-fi8pskod 210 4 3 3 CD cord-104231-fi8pskod 210 5 , , , cord-104231-fi8pskod 210 6 D D NNP cord-104231-fi8pskod 210 7 and and CC cord-104231-fi8pskod 210 8 H H NNP cord-104231-fi8pskod 210 9 , , , cord-104231-fi8pskod 210 10 respectively respectively RB cord-104231-fi8pskod 210 11 ) ) -RRB- cord-104231-fi8pskod 210 12 . . . cord-104231-fi8pskod 211 1 At at IN cord-104231-fi8pskod 211 2 all all DT cord-104231-fi8pskod 211 3 levels level NNS cord-104231-fi8pskod 211 4 of of IN cord-104231-fi8pskod 211 5 expression expression NN cord-104231-fi8pskod 211 6 staining staining NN cord-104231-fi8pskod 211 7 of of IN cord-104231-fi8pskod 211 8 small small JJ cord-104231-fi8pskod 211 9 punctate punctate NN cord-104231-fi8pskod 211 10 structures structure NNS cord-104231-fi8pskod 212 1 ( ( -LRB- cord-104231-fi8pskod 212 2 Fig Fig NNP cord-104231-fi8pskod 212 3 . . . cord-104231-fi8pskod 212 4 3 3 CD cord-104231-fi8pskod 212 5 , , , cord-104231-fi8pskod 212 6 C C NNP cord-104231-fi8pskod 212 7 and and CC cord-104231-fi8pskod 212 8 G G NNP cord-104231-fi8pskod 212 9 ) ) -RRB- cord-104231-fi8pskod 212 10 was be VBD cord-104231-fi8pskod 212 11 also also RB cord-104231-fi8pskod 212 12 observed observe VBN cord-104231-fi8pskod 212 13 and and CC cord-104231-fi8pskod 212 14 these these DT cord-104231-fi8pskod 212 15 were be VBD cord-104231-fi8pskod 212 16 shown show VBN cord-104231-fi8pskod 212 17 to to TO cord-104231-fi8pskod 212 18 be be VB cord-104231-fi8pskod 212 19 endosomes endosome NNS cord-104231-fi8pskod 212 20 by by IN cord-104231-fi8pskod 212 21 immunoelectron immunoelectron NNP cord-104231-fi8pskod 212 22 microscopy microscopy NNP cord-104231-fi8pskod 212 23 ( ( -LRB- cord-104231-fi8pskod 212 24 see see VB cord-104231-fi8pskod 212 25 below below RB cord-104231-fi8pskod 212 26 ) ) -RRB- cord-104231-fi8pskod 212 27 . . . cord-104231-fi8pskod 213 1 Replacing replace VBG cord-104231-fi8pskod 213 2 the the DT cord-104231-fi8pskod 213 3 membrane membrane NN cord-104231-fi8pskod 213 4 - - HYPH cord-104231-fi8pskod 213 5 spanning span VBG cord-104231-fi8pskod 213 6 domain domain NN cord-104231-fi8pskod 213 7 of of IN cord-104231-fi8pskod 213 8 either either DT cord-104231-fi8pskod 213 9 CIM CIM NNP cord-104231-fi8pskod 213 10 or or CC cord-104231-fi8pskod 213 11 CD8 CD8 NNP cord-104231-fi8pskod 213 12 with with IN cord-104231-fi8pskod 213 13 that that DT cord-104231-fi8pskod 213 14 of of IN cord-104231-fi8pskod 213 15 TGN38 tgn38 PRP cord-104231-fi8pskod 213 16 could could MD cord-104231-fi8pskod 213 17 have have VB cord-104231-fi8pskod 213 18 activated activate VBN cord-104231-fi8pskod 213 19 or or CC cord-104231-fi8pskod 213 20 corrupted corrupt VBN cord-104231-fi8pskod 213 21 a a DT cord-104231-fi8pskod 213 22 localization localization NN cord-104231-fi8pskod 213 23 signal signal NN cord-104231-fi8pskod 213 24 in in IN cord-104231-fi8pskod 213 25 the the DT cord-104231-fi8pskod 213 26 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 213 27 domain domain NN cord-104231-fi8pskod 213 28 of of IN cord-104231-fi8pskod 213 29 the the DT cord-104231-fi8pskod 213 30 hybrid hybrid JJ cord-104231-fi8pskod 213 31 proteins protein NNS cord-104231-fi8pskod 213 32 . . . cord-104231-fi8pskod 214 1 CD8 CD8 NNP cord-104231-fi8pskod 214 2 has have VBZ cord-104231-fi8pskod 214 3 a a DT cord-104231-fi8pskod 214 4 tyrosine tyrosine NN cord-104231-fi8pskod 214 5 residue residue NN cord-104231-fi8pskod 214 6 in in IN cord-104231-fi8pskod 214 7 the the DT cord-104231-fi8pskod 214 8 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 214 9 domain domain NN cord-104231-fi8pskod 214 10 but but CC cord-104231-fi8pskod 214 11 it -PRON- PRP cord-104231-fi8pskod 214 12 is be VBZ cord-104231-fi8pskod 214 13 the the DT cord-104231-fi8pskod 214 14 penultimate penultimate JJ cord-104231-fi8pskod 214 15 amino amino NN cord-104231-fi8pskod 214 16 acid acid NN cord-104231-fi8pskod 214 17 that that WDT cord-104231-fi8pskod 214 18 would would MD cord-104231-fi8pskod 214 19 not not RB cord-104231-fi8pskod 214 20 norreally norreally RB cord-104231-fi8pskod 214 21 be be VB cord-104231-fi8pskod 214 22 expected expect VBN cord-104231-fi8pskod 214 23 to to TO cord-104231-fi8pskod 214 24 operate operate VB cord-104231-fi8pskod 214 25 as as IN cord-104231-fi8pskod 214 26 a a DT cord-104231-fi8pskod 214 27 retrieval retrieval NN cord-104231-fi8pskod 214 28 signal signal NN cord-104231-fi8pskod 214 29 ( ( -LRB- cord-104231-fi8pskod 214 30 Fig Fig NNP cord-104231-fi8pskod 214 31 . . . cord-104231-fi8pskod 214 32 1 1 CD cord-104231-fi8pskod 214 33 A a CD cord-104231-fi8pskod 214 34 ) ) -RRB- cord-104231-fi8pskod 214 35 . . . cord-104231-fi8pskod 215 1 CD4 CD4 NNP cord-104231-fi8pskod 215 2 contains contain VBZ cord-104231-fi8pskod 215 3 a a DT cord-104231-fi8pskod 215 4 signal signal NN cord-104231-fi8pskod 215 5 for for IN cord-104231-fi8pskod 215 6 endocytosis endocytosis NN cord-104231-fi8pskod 215 7 ( ( -LRB- cord-104231-fi8pskod 215 8 Shin Shin NNP cord-104231-fi8pskod 215 9 et et FW cord-104231-fi8pskod 215 10 al al NNP cord-104231-fi8pskod 215 11 . . NNP cord-104231-fi8pskod 215 12 , , , cord-104231-fi8pskod 215 13 1990 1990 CD cord-104231-fi8pskod 215 14 ( ( -LRB- cord-104231-fi8pskod 215 15 Shin Shin NNP cord-104231-fi8pskod 215 16 et et NNP cord-104231-fi8pskod 215 17 al al NNP cord-104231-fi8pskod 215 18 . . NNP cord-104231-fi8pskod 215 19 , , , cord-104231-fi8pskod 215 20 , , , cord-104231-fi8pskod 215 21 1991 1991 CD cord-104231-fi8pskod 216 1 but but CC cord-104231-fi8pskod 216 2 this this DT cord-104231-fi8pskod 216 3 does do VBZ cord-104231-fi8pskod 216 4 not not RB cord-104231-fi8pskod 216 5 normally normally RB cord-104231-fi8pskod 216 6 direct direct VB cord-104231-fi8pskod 216 7 the the DT cord-104231-fi8pskod 216 8 protein protein NN cord-104231-fi8pskod 216 9 to to IN cord-104231-fi8pskod 216 10 the the DT cord-104231-fi8pskod 216 11 TGN TGN NNP cord-104231-fi8pskod 216 12 . . . cord-104231-fi8pskod 217 1 Nevertheless nevertheless RB cord-104231-fi8pskod 217 2 , , , cord-104231-fi8pskod 217 3 to to TO cord-104231-fi8pskod 217 4 eliminate eliminate VB cord-104231-fi8pskod 217 5 the the DT cord-104231-fi8pskod 217 6 possibility possibility NN cord-104231-fi8pskod 217 7 of of IN cord-104231-fi8pskod 217 8 activating activate VBG cord-104231-fi8pskod 217 9 or or CC cord-104231-fi8pskod 217 10 corrupting corrupt VBG cord-104231-fi8pskod 217 11 a a DT cord-104231-fi8pskod 217 12 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 217 13 signal signal NN cord-104231-fi8pskod 217 14 , , , cord-104231-fi8pskod 217 15 we -PRON- PRP cord-104231-fi8pskod 217 16 exploited exploit VBD cord-104231-fi8pskod 217 17 the the DT cord-104231-fi8pskod 217 18 fact fact NN cord-104231-fi8pskod 217 19 that that IN cord-104231-fi8pskod 217 20 the the DT cord-104231-fi8pskod 217 21 localization localization NN cord-104231-fi8pskod 217 22 signal signal NN cord-104231-fi8pskod 217 23 in in IN cord-104231-fi8pskod 217 24 the the DT cord-104231-fi8pskod 217 25 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 217 26 domain domain NN cord-104231-fi8pskod 217 27 of of IN cord-104231-fi8pskod 217 28 TGN38 TGN38 NNP cord-104231-fi8pskod 217 29 has have VBZ cord-104231-fi8pskod 217 30 been be VBN cord-104231-fi8pskod 217 31 mapped map VBN cord-104231-fi8pskod 217 32 to to IN cord-104231-fi8pskod 217 33 the the DT cord-104231-fi8pskod 217 34 sequence sequence NN cord-104231-fi8pskod 217 35 , , , cord-104231-fi8pskod 217 36 SDYQRL SDYQRL NNP cord-104231-fi8pskod 217 37 ( ( -LRB- cord-104231-fi8pskod 217 38 Bos Bos NNP cord-104231-fi8pskod 217 39 et et NNP cord-104231-fi8pskod 217 40 al al NNP cord-104231-fi8pskod 217 41 . . NNP cord-104231-fi8pskod 217 42 , , , cord-104231-fi8pskod 217 43 1993 1993 CD cord-104231-fi8pskod 217 44 ; ; : cord-104231-fi8pskod 218 1 Humphrey Humphrey NNP cord-104231-fi8pskod 218 2 et et NNP cord-104231-fi8pskod 218 3 al al NNP cord-104231-fi8pskod 218 4 . . . cord-104231-fi8pskod 219 1 , , , cord-104231-fi8pskod 219 2 1993 1993 CD cord-104231-fi8pskod 219 3 ; ; : cord-104231-fi8pskod 219 4 Wong Wong NNP cord-104231-fi8pskod 219 5 and and CC cord-104231-fi8pskod 219 6 Hong Hong NNP cord-104231-fi8pskod 219 7 , , , cord-104231-fi8pskod 219 8 1993 1993 CD cord-104231-fi8pskod 219 9 ) ) -RRB- cord-104231-fi8pskod 219 10 . . . cord-104231-fi8pskod 220 1 Comparison comparison NN cord-104231-fi8pskod 220 2 of of IN cord-104231-fi8pskod 220 3 the the DT cord-104231-fi8pskod 220 4 hybrid hybrid JJ cord-104231-fi8pskod 220 5 protein protein NN cord-104231-fi8pskod 220 6 CD8-MC CD8-MC NNP cord-104231-fi8pskod 220 7 ( ( -LRB- cord-104231-fi8pskod 220 8 Fig Fig NNP cord-104231-fi8pskod 220 9 . . . cord-104231-fi8pskod 220 10 4 4 CD cord-104231-fi8pskod 220 11 A a CD cord-104231-fi8pskod 220 12 ) ) -RRB- cord-104231-fi8pskod 220 13 with with IN cord-104231-fi8pskod 220 14 CD8-MAC CD8-MAC NNP cord-104231-fi8pskod 220 15 ( ( -LRB- cord-104231-fi8pskod 220 16 Fig Fig NNP cord-104231-fi8pskod 220 17 . . . cord-104231-fi8pskod 220 18 4 4 CD cord-104231-fi8pskod 220 19 B b NN cord-104231-fi8pskod 220 20 ) ) -RRB- cord-104231-fi8pskod 220 21 , , , cord-104231-fi8pskod 220 22 which which WDT cord-104231-fi8pskod 220 23 lacked lack VBD cord-104231-fi8pskod 220 24 this this DT cord-104231-fi8pskod 220 25 sequence sequence NN cord-104231-fi8pskod 220 26 , , , cord-104231-fi8pskod 220 27 showed show VBD cord-104231-fi8pskod 220 28 that that IN cord-104231-fi8pskod 220 29 it -PRON- PRP cord-104231-fi8pskod 220 30 had have VBD cord-104231-fi8pskod 220 31 no no DT cord-104231-fi8pskod 220 32 significant significant JJ cord-104231-fi8pskod 220 33 effect effect NN cord-104231-fi8pskod 220 34 on on IN cord-104231-fi8pskod 220 35 the the DT cord-104231-fi8pskod 220 36 localization localization NN cord-104231-fi8pskod 220 37 of of IN cord-104231-fi8pskod 220 38 the the DT cord-104231-fi8pskod 220 39 protein protein NN cord-104231-fi8pskod 220 40 to to IN cord-104231-fi8pskod 220 41 the the DT cord-104231-fi8pskod 220 42 Golgi Golgi NNP cord-104231-fi8pskod 220 43 apparatus apparatus NN cord-104231-fi8pskod 220 44 . . . cord-104231-fi8pskod 221 1 Both both DT cord-104231-fi8pskod 221 2 were be VBD cord-104231-fi8pskod 221 3 localized localize VBN cord-104231-fi8pskod 221 4 to to IN cord-104231-fi8pskod 221 5 a a DT cord-104231-fi8pskod 221 6 juxtanuclear juxtanuclear NN cord-104231-fi8pskod 221 7 reticulum reticulum NN cord-104231-fi8pskod 221 8 very very RB cord-104231-fi8pskod 221 9 similar similar JJ cord-104231-fi8pskod 221 10 to to IN cord-104231-fi8pskod 221 11 that that DT cord-104231-fi8pskod 221 12 stained stain VBN cord-104231-fi8pskod 221 13 by by IN cord-104231-fi8pskod 221 14 antibodies antibody NNS cord-104231-fi8pskod 221 15 to to IN cord-104231-fi8pskod 221 16 GaIT GaIT NNP cord-104231-fi8pskod 222 1 ( ( -LRB- cord-104231-fi8pskod 222 2 data datum NNS cord-104231-fi8pskod 222 3 not not RB cord-104231-fi8pskod 222 4 shown show VBN cord-104231-fi8pskod 222 5 ) ) -RRB- cord-104231-fi8pskod 222 6 . . . cord-104231-fi8pskod 223 1 Expression expression NN cord-104231-fi8pskod 223 2 of of IN cord-104231-fi8pskod 223 3 the the DT cord-104231-fi8pskod 223 4 CD4-MAC CD4-MAC NNP cord-104231-fi8pskod 223 5 hybrid hybrid NN cord-104231-fi8pskod 223 6 protein protein NN cord-104231-fi8pskod 223 7 gave give VBD cord-104231-fi8pskod 223 8 similar similar JJ cord-104231-fi8pskod 223 9 results result NNS cord-104231-fi8pskod 223 10 ( ( -LRB- cord-104231-fi8pskod 223 11 Fig fig NN cord-104231-fi8pskod 223 12 . . . cord-104231-fi8pskod 223 13 4 4 CD cord-104231-fi8pskod 223 14 E e NN cord-104231-fi8pskod 223 15 ) ) -RRB- cord-104231-fi8pskod 223 16 . . . cord-104231-fi8pskod 224 1 To to TO cord-104231-fi8pskod 224 2 show show VB cord-104231-fi8pskod 224 3 that that IN cord-104231-fi8pskod 224 4 we -PRON- PRP cord-104231-fi8pskod 224 5 had have VBD cord-104231-fi8pskod 224 6 in in IN cord-104231-fi8pskod 224 7 fact fact NN cord-104231-fi8pskod 224 8 eliminated eliminate VBD cord-104231-fi8pskod 224 9 the the DT cord-104231-fi8pskod 224 10 localization localization NN cord-104231-fi8pskod 224 11 signal signal NN cord-104231-fi8pskod 224 12 in in IN cord-104231-fi8pskod 224 13 the the DT cord-104231-fi8pskod 224 14 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 224 15 domain domain NN cord-104231-fi8pskod 224 16 of of IN cord-104231-fi8pskod 224 17 TGN38 TGN38 NNP cord-104231-fi8pskod 224 18 , , , cord-104231-fi8pskod 224 19 we -PRON- PRP cord-104231-fi8pskod 224 20 expressed express VBD cord-104231-fi8pskod 224 21 a a DT cord-104231-fi8pskod 224 22 CD8 CD8 NNP cord-104231-fi8pskod 224 23 or or CC cord-104231-fi8pskod 224 24 CD4 CD4 NNP cord-104231-fi8pskod 224 25 hybrid hybrid NN cord-104231-fi8pskod 224 26 protein protein NN cord-104231-fi8pskod 224 27 containing contain VBG cord-104231-fi8pskod 224 28 the the DT cord-104231-fi8pskod 224 29 truncated truncated JJ cord-104231-fi8pskod 224 30 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 224 31 domain domain NN cord-104231-fi8pskod 224 32 from from IN cord-104231-fi8pskod 224 33 TGN38 TGN38 NNS cord-104231-fi8pskod 224 34 ( ( -LRB- cord-104231-fi8pskod 224 35 CD8-AC cd8-ac NN cord-104231-fi8pskod 224 36 or or CC cord-104231-fi8pskod 224 37 CD4-~C cd4-~c NN cord-104231-fi8pskod 224 38 ; ; : cord-104231-fi8pskod 224 39 Fig fig NN cord-104231-fi8pskod 224 40 . . . cord-104231-fi8pskod 225 1 1 1 CD cord-104231-fi8pskod 225 2 _SP cord-104231-fi8pskod 225 3 B B NNP cord-104231-fi8pskod 225 4 ) ) -RRB- cord-104231-fi8pskod 225 5 . . . cord-104231-fi8pskod 226 1 Unlike unlike IN cord-104231-fi8pskod 226 2 CD8-C CD8-C NNP cord-104231-fi8pskod 226 3 ( ( -LRB- cord-104231-fi8pskod 226 4 see see VB cord-104231-fi8pskod 226 5 Fig Fig NNP cord-104231-fi8pskod 226 6 . . . cord-104231-fi8pskod 226 7 3 3 CD cord-104231-fi8pskod 226 8 C C NNP cord-104231-fi8pskod 226 9 ) ) -RRB- cord-104231-fi8pskod 226 10 , , , cord-104231-fi8pskod 226 11 the the DT cord-104231-fi8pskod 226 12 CD8-AC cd8-ac NN cord-104231-fi8pskod 226 13 hybrid hybrid NN cord-104231-fi8pskod 226 14 protein protein NN cord-104231-fi8pskod 226 15 was be VBD cord-104231-fi8pskod 226 16 not not RB cord-104231-fi8pskod 226 17 in in IN cord-104231-fi8pskod 226 18 the the DT cord-104231-fi8pskod 226 19 Golgi Golgi NNP cord-104231-fi8pskod 226 20 apparatus apparatus NN cord-104231-fi8pskod 226 21 but but CC cord-104231-fi8pskod 226 22 exhibited exhibit VBD cord-104231-fi8pskod 226 23 a a DT cord-104231-fi8pskod 226 24 cell cell NN cord-104231-fi8pskod 226 25 surface surface NN cord-104231-fi8pskod 226 26 staining staining NN cord-104231-fi8pskod 226 27 pattern pattern NN cord-104231-fi8pskod 226 28 and and CC cord-104231-fi8pskod 226 29 additional additional JJ cord-104231-fi8pskod 226 30 accumulation accumulation NN cord-104231-fi8pskod 226 31 in in IN cord-104231-fi8pskod 227 1 intracel- intracel- UH cord-104231-fi8pskod 227 2 * * NFP cord-104231-fi8pskod 228 1 The the DT cord-104231-fi8pskod 228 2 two two CD cord-104231-fi8pskod 228 3 categories category NNS cord-104231-fi8pskod 228 4 of of IN cord-104231-fi8pskod 228 5 transfected transfecte VBN cord-104231-fi8pskod 228 6 cells cell NNS cord-104231-fi8pskod 228 7 , , , cord-104231-fi8pskod 228 8 low low JJ cord-104231-fi8pskod 228 9 " " `` cord-104231-fi8pskod 228 10 expressers expresser NNS cord-104231-fi8pskod 228 11 " " '' cord-104231-fi8pskod 228 12 ( ( -LRB- cord-104231-fi8pskod 228 13 < < XX cord-104231-fi8pskod 228 14 ) ) -RRB- cord-104231-fi8pskod 228 15 and and CC cord-104231-fi8pskod 228 16 high high JJ cord-104231-fi8pskod 228 17 " " `` cord-104231-fi8pskod 228 18 expressers expresser NNS cord-104231-fi8pskod 228 19 ~ ~ NFP cord-104231-fi8pskod 228 20 ( ( -LRB- cord-104231-fi8pskod 228 21 > > NFP cord-104231-fi8pskod 228 22 ) ) -RRB- cord-104231-fi8pskod 228 23 , , , cord-104231-fi8pskod 228 24 were be VBD cord-104231-fi8pskod 228 25 defined define VBN cord-104231-fi8pskod 228 26 as as IN cord-104231-fi8pskod 228 27 described describe VBN cord-104231-fi8pskod 228 28 in in IN cord-104231-fi8pskod 228 29 Materials material NNS cord-104231-fi8pskod 228 30 and and CC cord-104231-fi8pskod 228 31 Methods method NNS cord-104231-fi8pskod 228 32 . . . cord-104231-fi8pskod 229 1 The the DT cord-104231-fi8pskod 229 2 total total JJ cord-104231-fi8pskod 229 3 number number NN cord-104231-fi8pskod 229 4 of of IN cord-104231-fi8pskod 229 5 gold gold NN cord-104231-fi8pskod 229 6 particles particle NNS cord-104231-fi8pskod 229 7 counted count VBN cord-104231-fi8pskod 229 8 was be VBD cord-104231-fi8pskod 229 9 1695 1695 CD cord-104231-fi8pskod 229 10 CrGN38 CrGN38 NNP cord-104231-fi8pskod 229 11 ) ) -RRB- cord-104231-fi8pskod 229 12 , , , cord-104231-fi8pskod 229 13 1036 1036 CD cord-104231-fi8pskod 229 14 ( ( -LRB- cord-104231-fi8pskod 229 15 CD4-M CD4-M NNP cord-104231-fi8pskod 229 16 ) ) -RRB- cord-104231-fi8pskod 229 17 , , , cord-104231-fi8pskod 229 18 and and CC cord-104231-fi8pskod 229 19 1302 1302 CD cord-104231-fi8pskod 229 20 ( ( -LRB- cord-104231-fi8pskod 229 21 CD4-C CD4-C NNP cord-104231-fi8pskod 229 22 ) ) -RRB- cord-104231-fi8pskod 229 23 . . . cord-104231-fi8pskod 230 1 * * NFP cord-104231-fi8pskod 230 2 The the DT cord-104231-fi8pskod 230 3 linear linear JJ cord-104231-fi8pskod 230 4 density density NN cord-104231-fi8pskod 230 5 at at IN cord-104231-fi8pskod 230 6 the the DT cord-104231-fi8pskod 230 7 plasma plasma NN cord-104231-fi8pskod 230 8 membrane membrane NN cord-104231-fi8pskod 230 9 and and CC cord-104231-fi8pskod 230 10 in in IN cord-104231-fi8pskod 230 11 the the DT cord-104231-fi8pskod 230 12 TGN TGN NNP cord-104231-fi8pskod 230 13 was be VBD cord-104231-fi8pskod 230 14 estimated estimate VBN cord-104231-fi8pskod 230 15 for for IN cord-104231-fi8pskod 230 16 the the DT cord-104231-fi8pskod 230 17 expressed express VBN cord-104231-fi8pskod 230 18 proteins protein NNS cord-104231-fi8pskod 230 19 as as IN cord-104231-fi8pskod 230 20 described describe VBN cord-104231-fi8pskod 230 21 in in IN cord-104231-fi8pskod 230 22 Materials material NNS cord-104231-fi8pskod 230 23 and and CC cord-104231-fi8pskod 230 24 Methods method NNS cord-104231-fi8pskod 230 25 . . . cord-104231-fi8pskod 231 1 The the DT cord-104231-fi8pskod 231 2 number number NN cord-104231-fi8pskod 231 3 of of IN cord-104231-fi8pskod 231 4 gold gold NN cord-104231-fi8pskod 231 5 particles particle NNS cord-104231-fi8pskod 231 6 counted count VBN cord-104231-fi8pskod 231 7 as as IN cord-104231-fi8pskod 231 8 the the DT cord-104231-fi8pskod 231 9 plasma plasma NN cord-104231-fi8pskod 231 10 membrane membrane NN cord-104231-fi8pskod 231 11 was be VBD cord-104231-fi8pskod 231 12 300 300 CD cord-104231-fi8pskod 231 13 ( ( -LRB- cord-104231-fi8pskod 231 14 TGN38 TGN38 NNP cord-104231-fi8pskod 231 15 ) ) -RRB- cord-104231-fi8pskod 231 16 , , , cord-104231-fi8pskod 231 17 70 70 CD cord-104231-fi8pskod 231 18 ( ( -LRB- cord-104231-fi8pskod 231 19 CIM CIM NNP cord-104231-fi8pskod 231 20 - - HYPH cord-104231-fi8pskod 231 21 M M NNP cord-104231-fi8pskod 231 22 ) ) -RRB- cord-104231-fi8pskod 231 23 , , , cord-104231-fi8pskod 231 24 and and CC cord-104231-fi8pskod 231 25 60 60 CD cord-104231-fi8pskod 231 26 ( ( -LRB- cord-104231-fi8pskod 231 27 CD4-C CD4-C NNP cord-104231-fi8pskod 231 28 ) ) -RRB- cord-104231-fi8pskod 231 29 . . . cord-104231-fi8pskod 232 1 Note note VB cord-104231-fi8pskod 232 2 that that DT cord-104231-fi8pskod 232 3 for for IN cord-104231-fi8pskod 232 4 TGN38 TGN38 NNP cord-104231-fi8pskod 232 5 and and CC cord-104231-fi8pskod 232 6 CIM CIM NNP cord-104231-fi8pskod 232 7 - - HYPH cord-104231-fi8pskod 232 8 M M NNP cord-104231-fi8pskod 232 9 , , , cord-104231-fi8pskod 232 10 gold gold NN cord-104231-fi8pskod 232 11 labeling labeling NN cord-104231-fi8pskod 232 12 at at IN cord-104231-fi8pskod 232 13 the the DT cord-104231-fi8pskod 232 14 plasma plasma NN cord-104231-fi8pskod 232 15 membrane membrane NN cord-104231-fi8pskod 232 16 correlated correlate VBD cord-104231-fi8pskod 232 17 with with IN cord-104231-fi8pskod 232 18 labeling labeling NN cord-104231-fi8pskod 232 19 of of IN cord-104231-fi8pskod 232 20 endosomes endosome NNS cord-104231-fi8pskod 232 21 . . . cord-104231-fi8pskod 233 1 lular lular NNP cord-104231-fi8pskod 233 2 punctate punctate NNP cord-104231-fi8pskod 233 3 structures structure NNS cord-104231-fi8pskod 234 1 ( ( -LRB- cord-104231-fi8pskod 234 2 Fig Fig NNP cord-104231-fi8pskod 234 3 . . . cord-104231-fi8pskod 234 4 4 4 CD cord-104231-fi8pskod 234 5 C C NNP cord-104231-fi8pskod 234 6 ) ) -RRB- cord-104231-fi8pskod 234 7 . . . cord-104231-fi8pskod 235 1 Some some DT cord-104231-fi8pskod 235 2 of of IN cord-104231-fi8pskod 235 3 these these DT cord-104231-fi8pskod 235 4 were be VBD cord-104231-fi8pskod 235 5 shown show VBN cord-104231-fi8pskod 235 6 to to TO cord-104231-fi8pskod 235 7 be be VB cord-104231-fi8pskod 235 8 early early JJ cord-104231-fi8pskod 235 9 endosomes endosome NNS cord-104231-fi8pskod 235 10 ( ( -LRB- cord-104231-fi8pskod 235 11 arrowheads arrowhead NNS cord-104231-fi8pskod 235 12 in in IN cord-104231-fi8pskod 235 13 Fig Fig NNP cord-104231-fi8pskod 235 14 . . . cord-104231-fi8pskod 236 1 4 4 LS cord-104231-fi8pskod 236 2 , , , cord-104231-fi8pskod 236 3 C C NNP cord-104231-fi8pskod 236 4 and and CC cord-104231-fi8pskod 236 5 D D NNP cord-104231-fi8pskod 236 6 ) ) -RRB- cord-104231-fi8pskod 236 7 by by IN cord-104231-fi8pskod 236 8 preincubating preincubate VBG cord-104231-fi8pskod 236 9 the the DT cord-104231-fi8pskod 236 10 cells cell NNS cord-104231-fi8pskod 236 11 with with IN cord-104231-fi8pskod 236 12 rhodamine rhodamine NN cord-104231-fi8pskod 236 13 - - HYPH cord-104231-fi8pskod 236 14 conjugated conjugate VBN cord-104231-fi8pskod 236 15 transferrin transferrin NN cord-104231-fi8pskod 236 16 for for IN cord-104231-fi8pskod 236 17 30 30 CD cord-104231-fi8pskod 236 18 min min NN cord-104231-fi8pskod 236 19 before before IN cord-104231-fi8pskod 236 20 fixation fixation NN cord-104231-fi8pskod 236 21 and and CC cord-104231-fi8pskod 236 22 processing processing NN cord-104231-fi8pskod 236 23 . . . cord-104231-fi8pskod 237 1 CD4-AC CD4-AC NNP cord-104231-fi8pskod 237 2 was be VBD cord-104231-fi8pskod 237 3 found find VBN cord-104231-fi8pskod 237 4 to to TO cord-104231-fi8pskod 237 5 be be VB cord-104231-fi8pskod 237 6 expressed express VBN cord-104231-fi8pskod 237 7 largely largely RB cord-104231-fi8pskod 237 8 on on IN cord-104231-fi8pskod 237 9 the the DT cord-104231-fi8pskod 237 10 cell cell NN cord-104231-fi8pskod 237 11 surface surface NN cord-104231-fi8pskod 237 12 of of IN cord-104231-fi8pskod 237 13 transfected transfecte VBN cord-104231-fi8pskod 237 14 cells cell NNS cord-104231-fi8pskod 237 15 ( ( -LRB- cord-104231-fi8pskod 237 16 Fig fig NN cord-104231-fi8pskod 237 17 . . . cord-104231-fi8pskod 237 18 4 4 CD cord-104231-fi8pskod 237 19 F F NNP cord-104231-fi8pskod 237 20 ) ) -RRB- cord-104231-fi8pskod 237 21 . . . cord-104231-fi8pskod 238 1 To to TO cord-104231-fi8pskod 238 2 determine determine VB cord-104231-fi8pskod 238 3 the the DT cord-104231-fi8pskod 238 4 precise precise JJ cord-104231-fi8pskod 238 5 location location NN cord-104231-fi8pskod 238 6 of of IN cord-104231-fi8pskod 238 7 the the DT cord-104231-fi8pskod 238 8 hybrid hybrid JJ cord-104231-fi8pskod 238 9 proteins protein NNS cord-104231-fi8pskod 238 10 , , , cord-104231-fi8pskod 238 11 cryosections cryosection NNS cord-104231-fi8pskod 238 12 of of IN cord-104231-fi8pskod 238 13 transfected transfecte VBN cord-104231-fi8pskod 238 14 cells cell NNS cord-104231-fi8pskod 238 15 were be VBD cord-104231-fi8pskod 238 16 labeled label VBN cord-104231-fi8pskod 238 17 with with IN cord-104231-fi8pskod 238 18 antibodies antibody NNS cord-104231-fi8pskod 238 19 which which WDT cord-104231-fi8pskod 238 20 were be VBD cord-104231-fi8pskod 238 21 detected detect VBN cord-104231-fi8pskod 238 22 by by IN cord-104231-fi8pskod 238 23 appropriate appropriate JJ cord-104231-fi8pskod 238 24 gold gold NN cord-104231-fi8pskod 238 25 conjugates conjugate NNS cord-104231-fi8pskod 238 26 . . . cord-104231-fi8pskod 239 1 The the DT cord-104231-fi8pskod 239 2 TGN TGN NNP cord-104231-fi8pskod 239 3 is be VBZ cord-104231-fi8pskod 239 4 defined define VBN cord-104231-fi8pskod 239 5 morphologically morphologically RB cord-104231-fi8pskod 239 6 as as IN cord-104231-fi8pskod 239 7 a a DT cord-104231-fi8pskod 239 8 tubulo tubulo JJ cord-104231-fi8pskod 239 9 - - HYPH cord-104231-fi8pskod 239 10 reticular reticular JJ cord-104231-fi8pskod 239 11 network network NN cord-104231-fi8pskod 239 12 , , , cord-104231-fi8pskod 239 13 part part NN cord-104231-fi8pskod 239 14 of of IN cord-104231-fi8pskod 239 15 which which WDT cord-104231-fi8pskod 239 16 is be VBZ cord-104231-fi8pskod 239 17 closely closely RB cord-104231-fi8pskod 239 18 apposed apposed JJ cord-104231-fi8pskod 239 19 to to IN cord-104231-fi8pskod 239 20 the the DT cord-104231-fi8pskod 239 21 trans trans JJ cord-104231-fi8pskod 239 22 side side NN cord-104231-fi8pskod 239 23 of of IN cord-104231-fi8pskod 239 24 the the DT cord-104231-fi8pskod 239 25 Golgi Golgi NNP cord-104231-fi8pskod 239 26 stack stack NN cord-104231-fi8pskod 239 27 . . . cord-104231-fi8pskod 240 1 Budding bud VBG cord-104231-fi8pskod 240 2 and and CC cord-104231-fi8pskod 240 3 vesicle vesicle NN cord-104231-fi8pskod 240 4 profiles profile NNS cord-104231-fi8pskod 240 5 coated coat VBN cord-104231-fi8pskod 240 6 by by IN cord-104231-fi8pskod 240 7 clathrin clathrin NN cord-104231-fi8pskod 240 8 are be VBP cord-104231-fi8pskod 240 9 often often RB cord-104231-fi8pskod 240 10 observed observe VBN cord-104231-fi8pskod 240 11 ( ( -LRB- cord-104231-fi8pskod 240 12 Orci Orci NNP cord-104231-fi8pskod 240 13 et et NNP cord-104231-fi8pskod 240 14 al al NNP cord-104231-fi8pskod 240 15 . . . cord-104231-fi8pskod 241 1 , , , cord-104231-fi8pskod 241 2 1984 1984 CD cord-104231-fi8pskod 241 3 ( ( -LRB- cord-104231-fi8pskod 241 4 Orci Orci NNP cord-104231-fi8pskod 241 5 et et NNP cord-104231-fi8pskod 241 6 al al NNP cord-104231-fi8pskod 241 7 . . . cord-104231-fi8pskod 242 1 , , , cord-104231-fi8pskod 242 2 , , , cord-104231-fi8pskod 242 3 1985 1985 CD cord-104231-fi8pskod 242 4 Griffiths Griffiths NNP cord-104231-fi8pskod 242 5 and and CC cord-104231-fi8pskod 242 6 Simons Simons NNP cord-104231-fi8pskod 242 7 , , , cord-104231-fi8pskod 242 8 1986 1986 CD cord-104231-fi8pskod 242 9 ) ) -RRB- cord-104231-fi8pskod 242 10 . . . cord-104231-fi8pskod 243 1 Transiently Transiently NNP cord-104231-fi8pskod 243 2 expressed express VBD cord-104231-fi8pskod 243 3 TGN38 TGN38 NNS cord-104231-fi8pskod 243 4 was be VBD cord-104231-fi8pskod 243 5 found find VBN cord-104231-fi8pskod 243 6 in in IN cord-104231-fi8pskod 243 7 the the DT cord-104231-fi8pskod 243 8 TGN TGN NNP cord-104231-fi8pskod 243 9 , , , cord-104231-fi8pskod 243 10 the the DT cord-104231-fi8pskod 243 11 Golgi Golgi NNP cord-104231-fi8pskod 243 12 stack stack NN cord-104231-fi8pskod 243 13 , , , cord-104231-fi8pskod 243 14 endosomes endosome NNS cord-104231-fi8pskod 243 15 , , , cord-104231-fi8pskod 243 16 and and CC cord-104231-fi8pskod 243 17 the the DT cord-104231-fi8pskod 243 18 plasma plasma NN cord-104231-fi8pskod 243 19 membrane membrane NN cord-104231-fi8pskod 243 20 ( ( -LRB- cord-104231-fi8pskod 243 21 Fig Fig NNP cord-104231-fi8pskod 243 22 . . . cord-104231-fi8pskod 243 23 5 5 CD cord-104231-fi8pskod 243 24 A a NN cord-104231-fi8pskod 243 25 ) ) -RRB- cord-104231-fi8pskod 243 26 . . . cord-104231-fi8pskod 244 1 The the DT cord-104231-fi8pskod 244 2 distribution distribution NN cord-104231-fi8pskod 244 3 of of IN cord-104231-fi8pskod 244 4 TGN38 TGN38 NNS cord-104231-fi8pskod 244 5 depended depend VBD cord-104231-fi8pskod 244 6 on on IN cord-104231-fi8pskod 244 7 the the DT cord-104231-fi8pskod 244 8 level level NN cord-104231-fi8pskod 244 9 of of IN cord-104231-fi8pskod 244 10 expression expression NN cord-104231-fi8pskod 244 11 . . . cord-104231-fi8pskod 245 1 At at IN cord-104231-fi8pskod 245 2 low low JJ cord-104231-fi8pskod 245 3 levels level NNS cord-104231-fi8pskod 245 4 ( ( -LRB- cord-104231-fi8pskod 245 5 < < XX cord-104231-fi8pskod 245 6 60 60 CD cord-104231-fi8pskod 245 7 gold gold NN cord-104231-fi8pskod 245 8 particles particle NNS cord-104231-fi8pskod 245 9 / / SYM cord-104231-fi8pskod 245 10 cell cell NN cord-104231-fi8pskod 245 11 section section NN cord-104231-fi8pskod 245 12 ) ) -RRB- cord-104231-fi8pskod 246 1 TGN38 TGN38 NNP cord-104231-fi8pskod 246 2 was be VBD cord-104231-fi8pskod 246 3 found find VBN cord-104231-fi8pskod 246 4 exclusively exclusively RB cord-104231-fi8pskod 246 5 in in IN cord-104231-fi8pskod 246 6 the the DT cord-104231-fi8pskod 246 7 Golgi Golgi NNP cord-104231-fi8pskod 246 8 apparatus apparatus NN cord-104231-fi8pskod 246 9 ( ( -LRB- cord-104231-fi8pskod 246 10 Table table NN cord-104231-fi8pskod 246 11 I -PRON- PRP cord-104231-fi8pskod 246 12 ) ) -RRB- cord-104231-fi8pskod 246 13 with with IN cord-104231-fi8pskod 246 14 most most JJS cord-104231-fi8pskod 246 15 of of IN cord-104231-fi8pskod 246 16 the the DT cord-104231-fi8pskod 246 17 protein protein NN cord-104231-fi8pskod 246 18 ( ( -LRB- cord-104231-fi8pskod 246 19 85 85 CD cord-104231-fi8pskod 246 20 + + SYM cord-104231-fi8pskod 246 21 7 7 CD cord-104231-fi8pskod 246 22 % % NN cord-104231-fi8pskod 246 23 ) ) -RRB- cord-104231-fi8pskod 246 24 being be VBG cord-104231-fi8pskod 246 25 found find VBN cord-104231-fi8pskod 246 26 in in IN cord-104231-fi8pskod 246 27 the the DT cord-104231-fi8pskod 246 28 TGN TGN NNP cord-104231-fi8pskod 246 29 ( ( -LRB- cord-104231-fi8pskod 246 30 Fig Fig NNP cord-104231-fi8pskod 246 31 . . . cord-104231-fi8pskod 246 32 6 6 CD cord-104231-fi8pskod 246 33 ) ) -RRB- cord-104231-fi8pskod 246 34 . . . cord-104231-fi8pskod 247 1 None none NN cord-104231-fi8pskod 247 2 was be VBD cord-104231-fi8pskod 247 3 detected detect VBN cord-104231-fi8pskod 247 4 in in IN cord-104231-fi8pskod 247 5 either either DT cord-104231-fi8pskod 247 6 endosomes endosome NNS cord-104231-fi8pskod 247 7 or or CC cord-104231-fi8pskod 247 8 on on IN cord-104231-fi8pskod 247 9 the the DT cord-104231-fi8pskod 247 10 plasma plasma NN cord-104231-fi8pskod 247 11 membrane membrane NN cord-104231-fi8pskod 247 12 ( ( -LRB- cord-104231-fi8pskod 247 13 Table table NN cord-104231-fi8pskod 247 14 I i CD cord-104231-fi8pskod 247 15 ) ) -RRB- cord-104231-fi8pskod 247 16 . . . cord-104231-fi8pskod 248 1 At at IN cord-104231-fi8pskod 248 2 higher high JJR cord-104231-fi8pskod 248 3 levels level NNS cord-104231-fi8pskod 248 4 of of IN cord-104231-fi8pskod 248 5 expression expression NN cord-104231-fi8pskod 248 6 ( ( -LRB- cord-104231-fi8pskod 248 7 > > SYM cord-104231-fi8pskod 248 8 60 60 CD cord-104231-fi8pskod 248 9 gold gold NN cord-104231-fi8pskod 248 10 particles particle NNS cord-104231-fi8pskod 248 11 / / SYM cord-104231-fi8pskod 248 12 cell cell NN cord-104231-fi8pskod 248 13 section section NN cord-104231-fi8pskod 248 14 ) ) -RRB- cord-104231-fi8pskod 248 15 the the DT cord-104231-fi8pskod 248 16 percentage percentage NN cord-104231-fi8pskod 248 17 of of IN cord-104231-fi8pskod 248 18 labeling labeling NN cord-104231-fi8pskod 248 19 over over IN cord-104231-fi8pskod 248 20 the the DT cord-104231-fi8pskod 248 21 Golgi Golgi NNP cord-104231-fi8pskod 248 22 apparatus apparatus NN cord-104231-fi8pskod 248 23 fell fall VBD cord-104231-fi8pskod 248 24 ( ( -LRB- cord-104231-fi8pskod 248 25 to to IN cord-104231-fi8pskod 248 26 60 60 CD cord-104231-fi8pskod 248 27 5:7 5:7 CD cord-104231-fi8pskod 248 28 % % NN cord-104231-fi8pskod 248 29 ) ) -RRB- cord-104231-fi8pskod 248 30 and and CC cord-104231-fi8pskod 248 31 rose rise VBD cord-104231-fi8pskod 248 32 over over IN cord-104231-fi8pskod 248 33 endosomes endosome NNS cord-104231-fi8pskod 248 34 ( ( -LRB- cord-104231-fi8pskod 248 35 to to IN cord-104231-fi8pskod 248 36 29 29 CD cord-104231-fi8pskod 248 37 5:7 5:7 CD cord-104231-fi8pskod 248 38 % % NN cord-104231-fi8pskod 248 39 ) ) -RRB- cord-104231-fi8pskod 248 40 and and CC cord-104231-fi8pskod 248 41 the the DT cord-104231-fi8pskod 248 42 plasma plasma NN cord-104231-fi8pskod 248 43 membrane membrane NN cord-104231-fi8pskod 248 44 ( ( -LRB- cord-104231-fi8pskod 248 45 to to IN cord-104231-fi8pskod 248 46 11 11 CD cord-104231-fi8pskod 248 47 + + SYM cord-104231-fi8pskod 248 48 7 7 CD cord-104231-fi8pskod 248 49 % % NN cord-104231-fi8pskod 248 50 ) ) -RRB- cord-104231-fi8pskod 249 1 ( ( -LRB- cord-104231-fi8pskod 249 2 Table table NN cord-104231-fi8pskod 249 3 I i CD cord-104231-fi8pskod 249 4 ) ) -RRB- cord-104231-fi8pskod 249 5 . . . cord-104231-fi8pskod 250 1 Even even RB cord-104231-fi8pskod 250 2 at at IN cord-104231-fi8pskod 250 3 high high JJ cord-104231-fi8pskod 250 4 levels level NNS cord-104231-fi8pskod 250 5 of of IN cord-104231-fi8pskod 250 6 expression expression NN cord-104231-fi8pskod 250 7 the the DT cord-104231-fi8pskod 250 8 linear linear JJ cord-104231-fi8pskod 250 9 density density NN cord-104231-fi8pskod 250 10 of of IN cord-104231-fi8pskod 250 11 labeling labeling NN cord-104231-fi8pskod 250 12 over over IN cord-104231-fi8pskod 250 13 the the DT cord-104231-fi8pskod 250 14 TGN TGN NNP cord-104231-fi8pskod 250 15 was be VBD cord-104231-fi8pskod 250 16 four four CD cord-104231-fi8pskod 250 17 times time NNS cord-104231-fi8pskod 250 18 that that IN cord-104231-fi8pskod 250 19 over over IN cord-104231-fi8pskod 250 20 the the DT cord-104231-fi8pskod 250 21 plasma plasma NN cord-104231-fi8pskod 250 22 membrane membrane NN cord-104231-fi8pskod 250 23 showing show VBG cord-104231-fi8pskod 250 24 that that IN cord-104231-fi8pskod 250 25 the the DT cord-104231-fi8pskod 250 26 protein protein NN cord-104231-fi8pskod 250 27 was be VBD cord-104231-fi8pskod 250 28 still still RB cord-104231-fi8pskod 250 29 concentrated concentrate VBN cord-104231-fi8pskod 250 30 in in IN cord-104231-fi8pskod 250 31 the the DT cord-104231-fi8pskod 250 32 TGN TGN NNP cord-104231-fi8pskod 250 33 . . . cord-104231-fi8pskod 251 1 Hybrid hybrid NN cord-104231-fi8pskod 251 2 proteins protein NNS cord-104231-fi8pskod 251 3 containing contain VBG cord-104231-fi8pskod 251 4 both both CC cord-104231-fi8pskod 251 5 localization localization NN cord-104231-fi8pskod 251 6 signals signal NNS cord-104231-fi8pskod 251 7 gave give VBD cord-104231-fi8pskod 251 8 patterns pattern NNS cord-104231-fi8pskod 251 9 of of IN cord-104231-fi8pskod 251 10 labeling label VBG cord-104231-fi8pskod 251 11 almost almost RB cord-104231-fi8pskod 251 12 indistinguishable indistinguishable JJ cord-104231-fi8pskod 251 13 from from IN cord-104231-fi8pskod 251 14 TGN38 tgn38 ADD cord-104231-fi8pskod 251 15 both both CC cord-104231-fi8pskod 251 16 morphologically morphologically RB cord-104231-fi8pskod 251 17 and and CC cord-104231-fi8pskod 251 18 quantitatively quantitatively RB cord-104231-fi8pskod 251 19 . . . cord-104231-fi8pskod 252 1 Both both DT cord-104231-fi8pskod 252 2 CD8-MC cd8-mc NN cord-104231-fi8pskod 252 3 ( ( -LRB- cord-104231-fi8pskod 252 4 Fig fig NN cord-104231-fi8pskod 252 5 . . . cord-104231-fi8pskod 252 6 5 5 CD cord-104231-fi8pskod 252 7 B b NN cord-104231-fi8pskod 252 8 ) ) -RRB- cord-104231-fi8pskod 252 9 and and CC cord-104231-fi8pskod 252 10 CD4-MC cd4-mc NN cord-104231-fi8pskod 252 11 ( ( -LRB- cord-104231-fi8pskod 252 12 Fig Fig NNP cord-104231-fi8pskod 252 13 . . . cord-104231-fi8pskod 252 14 5 5 CD cord-104231-fi8pskod 252 15 D d NN cord-104231-fi8pskod 252 16 ) ) -RRB- cord-104231-fi8pskod 252 17 were be VBD cord-104231-fi8pskod 252 18 present present JJ cord-104231-fi8pskod 252 19 only only RB cord-104231-fi8pskod 252 20 in in IN cord-104231-fi8pskod 252 21 the the DT cord-104231-fi8pskod 252 22 Golgi Golgi NNP cord-104231-fi8pskod 252 23 apparatus apparatus NN cord-104231-fi8pskod 252 24 at at IN cord-104231-fi8pskod 252 25 all all DT cord-104231-fi8pskod 252 26 levels level NNS cord-104231-fi8pskod 252 27 of of IN cord-104231-fi8pskod 252 28 expression expression NN cord-104231-fi8pskod 252 29 and and CC cord-104231-fi8pskod 252 30 more more JJR cord-104231-fi8pskod 252 31 than than IN cord-104231-fi8pskod 252 32 80 80 CD cord-104231-fi8pskod 252 33 % % NN cord-104231-fi8pskod 252 34 of of IN cord-104231-fi8pskod 252 35 the the DT cord-104231-fi8pskod 252 36 label label NN cord-104231-fi8pskod 252 37 was be VBD cord-104231-fi8pskod 252 38 present present JJ cord-104231-fi8pskod 252 39 in in IN cord-104231-fi8pskod 252 40 the the DT cord-104231-fi8pskod 252 41 TGN TGN NNP cord-104231-fi8pskod 252 42 ( ( -LRB- cord-104231-fi8pskod 252 43 Fig Fig NNP cord-104231-fi8pskod 252 44 . . . cord-104231-fi8pskod 252 45 6 6 CD cord-104231-fi8pskod 252 46 ) ) -RRB- cord-104231-fi8pskod 252 47 . . . cord-104231-fi8pskod 253 1 Hybrid hybrid NN cord-104231-fi8pskod 253 2 proteins protein NNS cord-104231-fi8pskod 253 3 containing contain VBG cord-104231-fi8pskod 253 4 only only RB cord-104231-fi8pskod 253 5 the the DT cord-104231-fi8pskod 253 6 membrane membrane NN cord-104231-fi8pskod 253 7 - - HYPH cord-104231-fi8pskod 253 8 spanning span VBG cord-104231-fi8pskod 253 9 domain domain NN cord-104231-fi8pskod 253 10 also also RB cord-104231-fi8pskod 253 11 gave give VBD cord-104231-fi8pskod 253 12 results result NNS cord-104231-fi8pskod 253 13 very very RB cord-104231-fi8pskod 253 14 similar similar JJ cord-104231-fi8pskod 253 15 to to IN cord-104231-fi8pskod 253 16 those those DT cord-104231-fi8pskod 253 17 for for IN cord-104231-fi8pskod 253 18 TGN38 TGN38 NNS cord-104231-fi8pskod 253 19 . . . cord-104231-fi8pskod 254 1 CD4-M CD4-M NNP cord-104231-fi8pskod 254 2 was be VBD cord-104231-fi8pskod 254 3 present present JJ cord-104231-fi8pskod 254 4 only only RB cord-104231-fi8pskod 254 5 in in IN cord-104231-fi8pskod 254 6 the the DT cord-104231-fi8pskod 254 7 Golgi Golgi NNP cord-104231-fi8pskod 254 8 apparatus apparatus NN cord-104231-fi8pskod 254 9 at at IN cord-104231-fi8pskod 254 10 low low JJ cord-104231-fi8pskod 254 11 levels level NNS cord-104231-fi8pskod 254 12 of of IN cord-104231-fi8pskod 254 13 expression expression NN cord-104231-fi8pskod 254 14 ( ( -LRB- cord-104231-fi8pskod 254 15 < < XX cord-104231-fi8pskod 254 16 50 50 CD cord-104231-fi8pskod 254 17 gold gold NN cord-104231-fi8pskod 254 18 particles particle NNS cord-104231-fi8pskod 254 19 / / SYM cord-104231-fi8pskod 254 20 cell cell NN cord-104231-fi8pskod 254 21 section section NN cord-104231-fi8pskod 254 22 ; ; : cord-104231-fi8pskod 254 23 Table Table NNP cord-104231-fi8pskod 254 24 I i CD cord-104231-fi8pskod 254 25 ) ) -RRB- cord-104231-fi8pskod 254 26 with with IN cord-104231-fi8pskod 254 27 80 80 CD cord-104231-fi8pskod 254 28 5:8 5:8 CD cord-104231-fi8pskod 254 29 % % NN cord-104231-fi8pskod 254 30 being be VBG cord-104231-fi8pskod 254 31 present present JJ cord-104231-fi8pskod 254 32 in in IN cord-104231-fi8pskod 254 33 the the DT cord-104231-fi8pskod 254 34 TGN TGN NNP cord-104231-fi8pskod 254 35 ( ( -LRB- cord-104231-fi8pskod 254 36 Fig Fig NNP cord-104231-fi8pskod 254 37 . . . cord-104231-fi8pskod 254 38 6 6 CD cord-104231-fi8pskod 254 39 ) ) -RRB- cord-104231-fi8pskod 254 40 . . . cord-104231-fi8pskod 255 1 At at IN cord-104231-fi8pskod 255 2 higher high JJR cord-104231-fi8pskod 255 3 levels level NNS cord-104231-fi8pskod 255 4 of of IN cord-104231-fi8pskod 255 5 expression expression NN cord-104231-fi8pskod 255 6 ( ( -LRB- cord-104231-fi8pskod 255 7 > > NFP cord-104231-fi8pskod 255 8 50 50 CD cord-104231-fi8pskod 255 9 gold gold NN cord-104231-fi8pskod 255 10 particles particle NNS cord-104231-fi8pskod 255 11 / / SYM cord-104231-fi8pskod 255 12 cell cell NN cord-104231-fi8pskod 255 13 section section NN cord-104231-fi8pskod 255 14 ) ) -RRB- cord-104231-fi8pskod 255 15 the the DT cord-104231-fi8pskod 255 16 percentage percentage NN cord-104231-fi8pskod 255 17 of of IN cord-104231-fi8pskod 255 18 total total JJ cord-104231-fi8pskod 255 19 labeling labeling NN cord-104231-fi8pskod 255 20 over over IN cord-104231-fi8pskod 255 21 the the DT cord-104231-fi8pskod 255 22 Golgi Golgi NNP cord-104231-fi8pskod 255 23 apparatus apparatus NN cord-104231-fi8pskod 255 24 fell fall VBD cord-104231-fi8pskod 255 25 ( ( -LRB- cord-104231-fi8pskod 255 26 to to IN cord-104231-fi8pskod 255 27 61 61 CD cord-104231-fi8pskod 255 28 5 5 CD cord-104231-fi8pskod 255 29 : : : cord-104231-fi8pskod 255 30 8 8 CD cord-104231-fi8pskod 255 31 % % NN cord-104231-fi8pskod 255 32 ) ) -RRB- cord-104231-fi8pskod 255 33 and and CC cord-104231-fi8pskod 255 34 rose rise VBD cord-104231-fi8pskod 255 35 over over IN cord-104231-fi8pskod 255 36 endosomes endosome NNS cord-104231-fi8pskod 255 37 ( ( -LRB- cord-104231-fi8pskod 255 38 to to IN cord-104231-fi8pskod 255 39 35 35 CD cord-104231-fi8pskod 255 40 + + SYM cord-104231-fi8pskod 255 41 8 8 CD cord-104231-fi8pskod 255 42 % % NN cord-104231-fi8pskod 255 43 ) ) -RRB- cord-104231-fi8pskod 255 44 and and CC cord-104231-fi8pskod 255 45 plasma plasma NN cord-104231-fi8pskod 255 46 membrane membrane NN cord-104231-fi8pskod 255 47 ( ( -LRB- cord-104231-fi8pskod 255 48 to to IN cord-104231-fi8pskod 255 49 4 4 CD cord-104231-fi8pskod 255 50 + + SYM cord-104231-fi8pskod 255 51 3 3 CD cord-104231-fi8pskod 255 52 % % NN cord-104231-fi8pskod 255 53 ) ) -RRB- cord-104231-fi8pskod 256 1 ( ( -LRB- cord-104231-fi8pskod 256 2 Table table NN cord-104231-fi8pskod 256 3 I i CD cord-104231-fi8pskod 256 4 ) ) -RRB- cord-104231-fi8pskod 256 5 . . . cord-104231-fi8pskod 257 1 This this DT cord-104231-fi8pskod 257 2 distribution distribution NN cord-104231-fi8pskod 257 3 was be VBD cord-104231-fi8pskod 257 4 not not RB cord-104231-fi8pskod 257 5 affected affect VBN cord-104231-fi8pskod 257 6 by by IN cord-104231-fi8pskod 257 7 pretreatment pretreatment NN cord-104231-fi8pskod 257 8 of of IN cord-104231-fi8pskod 257 9 the the DT cord-104231-fi8pskod 257 10 cells cell NNS cord-104231-fi8pskod 257 11 for for IN cord-104231-fi8pskod 257 12 2 2 CD cord-104231-fi8pskod 257 13 h h NN cord-104231-fi8pskod 257 14 with with IN cord-104231-fi8pskod 257 15 cycloheximide cycloheximide NN cord-104231-fi8pskod 257 16 ( ( -LRB- cord-104231-fi8pskod 257 17 Fig fig NN cord-104231-fi8pskod 257 18 . . . cord-104231-fi8pskod 257 19 5 5 CD cord-104231-fi8pskod 257 20 F F NNP cord-104231-fi8pskod 257 21 ) ) -RRB- cord-104231-fi8pskod 257 22 . . . cord-104231-fi8pskod 258 1 The the DT cord-104231-fi8pskod 258 2 linear linear JJ cord-104231-fi8pskod 258 3 density density NN cord-104231-fi8pskod 258 4 of of IN cord-104231-fi8pskod 258 5 labeling labeling NN cord-104231-fi8pskod 258 6 over over IN cord-104231-fi8pskod 258 7 the the DT cord-104231-fi8pskod 258 8 TGN TGN NNP cord-104231-fi8pskod 258 9 was be VBD cord-104231-fi8pskod 258 10 11 11 CD cord-104231-fi8pskod 258 11 times time NNS cord-104231-fi8pskod 258 12 that that IN cord-104231-fi8pskod 258 13 over over IN cord-104231-fi8pskod 258 14 the the DT cord-104231-fi8pskod 258 15 plasma plasma NN cord-104231-fi8pskod 258 16 membrane membrane NN cord-104231-fi8pskod 258 17 , , , cord-104231-fi8pskod 258 18 even even RB cord-104231-fi8pskod 258 19 higher high JJR cord-104231-fi8pskod 258 20 than than IN cord-104231-fi8pskod 258 21 that that DT cord-104231-fi8pskod 258 22 for for IN cord-104231-fi8pskod 258 23 TGN38 tgn38 ADD cord-104231-fi8pskod 259 1 ( ( -LRB- cord-104231-fi8pskod 259 2 Table table NN cord-104231-fi8pskod 259 3 I i CD cord-104231-fi8pskod 259 4 ) ) -RRB- cord-104231-fi8pskod 259 5 . . . cord-104231-fi8pskod 260 1 The the DT cord-104231-fi8pskod 260 2 other other JJ cord-104231-fi8pskod 260 3 hybrid hybrid JJ cord-104231-fi8pskod 260 4 protein protein NN cord-104231-fi8pskod 260 5 , , , cord-104231-fi8pskod 260 6 CDS CDS NNP cord-104231-fi8pskod 260 7 - - HYPH cord-104231-fi8pskod 260 8 M M NNP cord-104231-fi8pskod 260 9 , , , cord-104231-fi8pskod 260 10 was be VBD cord-104231-fi8pskod 260 11 also also RB cord-104231-fi8pskod 260 12 present present JJ cord-104231-fi8pskod 260 13 exclusively exclusively RB cord-104231-fi8pskod 260 14 in in IN cord-104231-fi8pskod 260 15 the the DT cord-104231-fi8pskod 260 16 Golgi Golgi NNP cord-104231-fi8pskod 260 17 apparatus apparatus NN cord-104231-fi8pskod 260 18 ( ( -LRB- cord-104231-fi8pskod 260 19 Fig Fig NNP cord-104231-fi8pskod 260 20 . . . cord-104231-fi8pskod 260 21 5 5 CD cord-104231-fi8pskod 260 22 C C NNP cord-104231-fi8pskod 260 23 ) ) -RRB- cord-104231-fi8pskod 260 24 and and CC cord-104231-fi8pskod 260 25 most most JJS cord-104231-fi8pskod 260 26 ( ( -LRB- cord-104231-fi8pskod 260 27 66 66 CD cord-104231-fi8pskod 260 28 5 5 CD cord-104231-fi8pskod 260 29 : : : cord-104231-fi8pskod 260 30 8 8 CD cord-104231-fi8pskod 260 31 % % NN cord-104231-fi8pskod 260 32 ) ) -RRB- cord-104231-fi8pskod 260 33 was be VBD cord-104231-fi8pskod 260 34 present present JJ cord-104231-fi8pskod 260 35 in in IN cord-104231-fi8pskod 260 36 the the DT cord-104231-fi8pskod 260 37 TGN TGN NNP cord-104231-fi8pskod 260 38 ( ( -LRB- cord-104231-fi8pskod 260 39 Fig Fig NNP cord-104231-fi8pskod 260 40 . . . cord-104231-fi8pskod 260 41 _SP cord-104231-fi8pskod 260 42 6 6 CD cord-104231-fi8pskod 260 43 ) ) -RRB- cord-104231-fi8pskod 260 44 . . . cord-104231-fi8pskod 261 1 However however RB cord-104231-fi8pskod 261 2 , , , cord-104231-fi8pskod 261 3 more more JJR cord-104231-fi8pskod 261 4 of of IN cord-104231-fi8pskod 261 5 the the DT cord-104231-fi8pskod 261 6 protein protein NN cord-104231-fi8pskod 261 7 was be VBD cord-104231-fi8pskod 261 8 present present JJ cord-104231-fi8pskod 261 9 in in IN cord-104231-fi8pskod 261 10 the the DT cord-104231-fi8pskod 261 11 Golgi Golgi NNP cord-104231-fi8pskod 261 12 stack stack NN cord-104231-fi8pskod 261 13 than than IN cord-104231-fi8pskod 261 14 for for IN cord-104231-fi8pskod 261 15 other other JJ cord-104231-fi8pskod 261 16 hybrid hybrid JJ cord-104231-fi8pskod 261 17 proteins protein NNS cord-104231-fi8pskod 261 18 . . . cord-104231-fi8pskod 262 1 We -PRON- PRP cord-104231-fi8pskod 262 2 also also RB cord-104231-fi8pskod 262 3 examined examine VBD cord-104231-fi8pskod 262 4 cells cell NNS cord-104231-fi8pskod 262 5 expressing express VBG cord-104231-fi8pskod 262 6 CD4-M CD4-M NNP cord-104231-fi8pskod 262 7 after after IN cord-104231-fi8pskod 262 8 incubation incubation NN cord-104231-fi8pskod 262 9 in in IN cord-104231-fi8pskod 262 10 cycloheximide cycloheximide NN cord-104231-fi8pskod 262 11 for for IN cord-104231-fi8pskod 262 12 7 7 CD cord-104231-fi8pskod 262 13 h h NN cord-104231-fi8pskod 262 14 and and CC cord-104231-fi8pskod 262 15 found find VBD cord-104231-fi8pskod 262 16 no no DT cord-104231-fi8pskod 262 17 significant significant JJ cord-104231-fi8pskod 262 18 change change NN cord-104231-fi8pskod 262 19 in in IN cord-104231-fi8pskod 262 20 the the DT cord-104231-fi8pskod 262 21 pattern pattern NN cord-104231-fi8pskod 262 22 of of IN cord-104231-fi8pskod 262 23 labeling labeling NN cord-104231-fi8pskod 262 24 ( ( -LRB- cord-104231-fi8pskod 262 25 data datum NNS cord-104231-fi8pskod 262 26 not not RB cord-104231-fi8pskod 262 27 shown show VBN cord-104231-fi8pskod 262 28 ) ) -RRB- cord-104231-fi8pskod 262 29 . . . cord-104231-fi8pskod 263 1 The the DT cord-104231-fi8pskod 263 2 CD4 CD4 NNP cord-104231-fi8pskod 263 3 hybrid hybrid NN cord-104231-fi8pskod 263 4 protein protein NN cord-104231-fi8pskod 263 5 containing contain VBG cord-104231-fi8pskod 263 6 the the DT cord-104231-fi8pskod 263 7 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 263 8 domain domain NN cord-104231-fi8pskod 263 9 ( ( -LRB- cord-104231-fi8pskod 263 10 CD4-C CD4-C NNP cord-104231-fi8pskod 263 11 ) ) -RRB- cord-104231-fi8pskod 263 12 was be VBD cord-104231-fi8pskod 263 13 also also RB cord-104231-fi8pskod 263 14 localized localize VBN cord-104231-fi8pskod 263 15 using use VBG cord-104231-fi8pskod 263 16 immunoelectron immunoelectron NNP cord-104231-fi8pskod 263 17 microscopy microscopy NNP cord-104231-fi8pskod 263 18 . . . cord-104231-fi8pskod 264 1 CD8-C CD8-C NNP cord-104231-fi8pskod 264 2 could could MD cord-104231-fi8pskod 264 3 not not RB cord-104231-fi8pskod 264 4 be be VB cord-104231-fi8pskod 264 5 detected detect VBN cord-104231-fi8pskod 264 6 by by IN cord-104231-fi8pskod 264 7 immuno immuno JJ cord-104231-fi8pskod 264 8 - - HYPH cord-104231-fi8pskod 264 9 electron electron NN cord-104231-fi8pskod 264 10 microscopy microscopy NN cord-104231-fi8pskod 264 11 for for IN cord-104231-fi8pskod 264 12 reasons reason NNS cord-104231-fi8pskod 264 13 that that WDT cord-104231-fi8pskod 264 14 are be VBP cord-104231-fi8pskod 264 15 still still RB cord-104231-fi8pskod 264 16 unclear unclear JJ cord-104231-fi8pskod 264 17 . . . cord-104231-fi8pskod 265 1 More More JJR cord-104231-fi8pskod 265 2 than than IN cord-104231-fi8pskod 265 3 84 84 CD cord-104231-fi8pskod 265 4 % % NN cord-104231-fi8pskod 265 5 of of IN cord-104231-fi8pskod 265 6 the the DT cord-104231-fi8pskod 265 7 CD4-C CD4-C NNP cord-104231-fi8pskod 265 8 in in IN cord-104231-fi8pskod 265 9 the the DT cord-104231-fi8pskod 265 10 Golgi Golgi NNP cord-104231-fi8pskod 265 11 apparatus apparatus NN cord-104231-fi8pskod 265 12 was be VBD cord-104231-fi8pskod 265 13 present present JJ cord-104231-fi8pskod 265 14 in in IN cord-104231-fi8pskod 265 15 the the DT cord-104231-fi8pskod 265 16 TGN TGN NNP cord-104231-fi8pskod 265 17 ( ( -LRB- cord-104231-fi8pskod 265 18 Fig Fig NNP cord-104231-fi8pskod 265 19 . . . cord-104231-fi8pskod 265 20 6 6 CD cord-104231-fi8pskod 265 21 ) ) -RRB- cord-104231-fi8pskod 266 1 The the DT cord-104231-fi8pskod 266 2 Journal Journal NNP cord-104231-fi8pskod 266 3 of of IN cord-104231-fi8pskod 266 4 Cell Cell NNP cord-104231-fi8pskod 266 5 Biology Biology NNP cord-104231-fi8pskod 266 6 , , , cord-104231-fi8pskod 266 7 Volume volume NN cord-104231-fi8pskod 266 8 125 125 CD cord-104231-fi8pskod 266 9 , , , cord-104231-fi8pskod 266 10 1994 1994 CD cord-104231-fi8pskod 266 11 5 5 CD cord-104231-fi8pskod 266 12 E e NN cord-104231-fi8pskod 266 13 ) ) -RRB- cord-104231-fi8pskod 266 14 . . . cord-104231-fi8pskod 267 1 However however RB cord-104231-fi8pskod 267 2 , , , cord-104231-fi8pskod 267 3 CD4-C CD4-C NNP cord-104231-fi8pskod 267 4 differed differ VBD cord-104231-fi8pskod 267 5 from from IN cord-104231-fi8pskod 267 6 other other JJ cord-104231-fi8pskod 267 7 hybrid hybrid JJ cord-104231-fi8pskod 267 8 proteins protein NNS cord-104231-fi8pskod 267 9 and and CC cord-104231-fi8pskod 267 10 TGN38 TGN38 NNS cord-104231-fi8pskod 267 11 in in IN cord-104231-fi8pskod 267 12 three three CD cord-104231-fi8pskod 267 13 respects respect NNS cord-104231-fi8pskod 267 14 . . . cord-104231-fi8pskod 268 1 First first RB cord-104231-fi8pskod 268 2 , , , cord-104231-fi8pskod 268 3 more more JJR cord-104231-fi8pskod 268 4 than than IN cord-104231-fi8pskod 268 5 35 35 CD cord-104231-fi8pskod 268 6 % % NN cord-104231-fi8pskod 268 7 of of IN cord-104231-fi8pskod 268 8 the the DT cord-104231-fi8pskod 268 9 total total JJ cord-104231-fi8pskod 268 10 protein protein NN cord-104231-fi8pskod 268 11 was be VBD cord-104231-fi8pskod 268 12 present present JJ cord-104231-fi8pskod 268 13 in in IN cord-104231-fi8pskod 268 14 endosomal endosomal JJ cord-104231-fi8pskod 268 15 structures structure NNS cord-104231-fi8pskod 268 16 at at IN cord-104231-fi8pskod 268 17 low low JJ cord-104231-fi8pskod 268 18 levels level NNS cord-104231-fi8pskod 268 19 of of IN cord-104231-fi8pskod 268 20 expression expression NN cord-104231-fi8pskod 268 21 and and CC cord-104231-fi8pskod 268 22 this this DT cord-104231-fi8pskod 268 23 increased increase VBD cord-104231-fi8pskod 268 24 to to IN cord-104231-fi8pskod 268 25 nearly nearly RB cord-104231-fi8pskod 268 26 60 60 CD cord-104231-fi8pskod 268 27 % % NN cord-104231-fi8pskod 268 28 at at IN cord-104231-fi8pskod 268 29 high high JJ cord-104231-fi8pskod 268 30 levels level NNS cord-104231-fi8pskod 268 31 . . . cord-104231-fi8pskod 269 1 Second second RB cord-104231-fi8pskod 269 2 , , , cord-104231-fi8pskod 269 3 the the DT cord-104231-fi8pskod 269 4 type type NN cord-104231-fi8pskod 269 5 of of IN cord-104231-fi8pskod 269 6 endosomal endosomal NNP cord-104231-fi8pskod 269 7 structure structure NN cord-104231-fi8pskod 269 8 labeled label VBN cord-104231-fi8pskod 269 9 also also RB cord-104231-fi8pskod 269 10 changed change VBD cord-104231-fi8pskod 269 11 . . . cord-104231-fi8pskod 270 1 At at IN cord-104231-fi8pskod 270 2 low low JJ cord-104231-fi8pskod 270 3 levels level NNS cord-104231-fi8pskod 270 4 , , , cord-104231-fi8pskod 270 5 all all DT cord-104231-fi8pskod 270 6 of of IN cord-104231-fi8pskod 270 7 the the DT cord-104231-fi8pskod 270 8 endosomal endosomal NNP cord-104231-fi8pskod 270 9 structures structure NNS cord-104231-fi8pskod 270 10 were be VBD cord-104231-fi8pskod 270 11 multivesicular multivesicular JJ cord-104231-fi8pskod 270 12 bodies body NNS cord-104231-fi8pskod 270 13 which which WDT cord-104231-fi8pskod 270 14 are be VBP cord-104231-fi8pskod 270 15 late late RB cord-104231-fi8pskod 270 16 endosomal endosomal NNP cord-104231-fi8pskod 270 17 compartments compartment NNS cord-104231-fi8pskod 270 18 ( ( -LRB- cord-104231-fi8pskod 270 19 Geuze Geuze NNP cord-104231-fi8pskod 270 20 et et NNP cord-104231-fi8pskod 270 21 al al NNP cord-104231-fi8pskod 270 22 . . NNP cord-104231-fi8pskod 270 23 , , , cord-104231-fi8pskod 270 24 1988 1988 CD cord-104231-fi8pskod 270 25 ; ; : cord-104231-fi8pskod 270 26 Hopkins Hopkins NNP cord-104231-fi8pskod 270 27 et et NNP cord-104231-fi8pskod 270 28 al al NNP cord-104231-fi8pskod 270 29 . . NNP cord-104231-fi8pskod 270 30 , , , cord-104231-fi8pskod 270 31 1990 1990 CD cord-104231-fi8pskod 270 32 ) ) -RRB- cord-104231-fi8pskod 270 33 . . . cord-104231-fi8pskod 271 1 At at IN cord-104231-fi8pskod 271 2 high high JJ cord-104231-fi8pskod 271 3 levels level NNS cord-104231-fi8pskod 271 4 of of IN cord-104231-fi8pskod 271 5 expression expression NN cord-104231-fi8pskod 271 6 , , , cord-104231-fi8pskod 271 7 labeling labeling NN cord-104231-fi8pskod 271 8 was be VBD cord-104231-fi8pskod 271 9 also also RB cord-104231-fi8pskod 271 10 present present JJ cord-104231-fi8pskod 271 11 in in IN cord-104231-fi8pskod 271 12 tubular tubular JJ cord-104231-fi8pskod 271 13 endosomes endosome NNS cord-104231-fi8pskod 271 14 . . . cord-104231-fi8pskod 272 1 Because because IN cord-104231-fi8pskod 272 2 of of IN cord-104231-fi8pskod 272 3 the the DT cord-104231-fi8pskod 272 4 difficulties difficulty NNS cord-104231-fi8pskod 272 5 of of IN cord-104231-fi8pskod 272 6 distinguishing distinguish VBG cord-104231-fi8pskod 272 7 between between IN cord-104231-fi8pskod 272 8 tubular tubular JJ cord-104231-fi8pskod 272 9 endosomes endosome NNS cord-104231-fi8pskod 272 10 and and CC cord-104231-fi8pskod 272 11 the the DT cord-104231-fi8pskod 272 12 TGN TGN NNP cord-104231-fi8pskod 272 13 ( ( -LRB- cord-104231-fi8pskod 272 14 Geuze Geuze NNP cord-104231-fi8pskod 272 15 et et NNP cord-104231-fi8pskod 272 16 al al NNP cord-104231-fi8pskod 272 17 . . NNP cord-104231-fi8pskod 272 18 , , , cord-104231-fi8pskod 272 19 1988 1988 CD cord-104231-fi8pskod 272 20 ; ; : cord-104231-fi8pskod 272 21 Klumperman Klumperman NNP cord-104231-fi8pskod 272 22 et et FW cord-104231-fi8pskod 272 23 al al NNP cord-104231-fi8pskod 272 24 . . NNP cord-104231-fi8pskod 272 25 , , , cord-104231-fi8pskod 272 26 1993 1993 CD cord-104231-fi8pskod 272 27 ) ) -RRB- cord-104231-fi8pskod 272 28 , , , cord-104231-fi8pskod 272 29 definitive definitive JJ cord-104231-fi8pskod 272 30 identification identification NN cord-104231-fi8pskod 272 31 of of IN cord-104231-fi8pskod 272 32 these these DT cord-104231-fi8pskod 272 33 structures structure NNS cord-104231-fi8pskod 272 34 will will MD cord-104231-fi8pskod 272 35 have have VB cord-104231-fi8pskod 272 36 to to TO cord-104231-fi8pskod 272 37 await await VB cord-104231-fi8pskod 272 38 further further JJ cord-104231-fi8pskod 272 39 analysis analysis NN cord-104231-fi8pskod 272 40 using use VBG cord-104231-fi8pskod 272 41 stably stably RB cord-104231-fi8pskod 272 42 transfected transfecte VBN cord-104231-fi8pskod 272 43 cell cell NN cord-104231-fi8pskod 272 44 lines line NNS cord-104231-fi8pskod 272 45 . . . cord-104231-fi8pskod 273 1 Third third JJ cord-104231-fi8pskod 273 2 and and CC cord-104231-fi8pskod 273 3 last last JJ cord-104231-fi8pskod 273 4 , , , cord-104231-fi8pskod 273 5 the the DT cord-104231-fi8pskod 273 6 plasma plasma NN cord-104231-fi8pskod 273 7 membrane membrane NN cord-104231-fi8pskod 273 8 was be VBD cord-104231-fi8pskod 273 9 labeled label VBN cord-104231-fi8pskod 273 10 at at IN cord-104231-fi8pskod 273 11 both both CC cord-104231-fi8pskod 273 12 high high JJ cord-104231-fi8pskod 273 13 and and CC cord-104231-fi8pskod 273 14 low low JJ cord-104231-fi8pskod 273 15 levels level NNS cord-104231-fi8pskod 273 16 of of IN cord-104231-fi8pskod 273 17 expression expression NN cord-104231-fi8pskod 273 18 ( ( -LRB- cord-104231-fi8pskod 273 19 Table table NN cord-104231-fi8pskod 273 20 _SP cord-104231-fi8pskod 273 21 I i NN cord-104231-fi8pskod 273 22 ) ) -RRB- cord-104231-fi8pskod 273 23 . . . cord-104231-fi8pskod 274 1 The the DT cord-104231-fi8pskod 274 2 linear linear JJ cord-104231-fi8pskod 274 3 density density NN cord-104231-fi8pskod 274 4 was be VBD cord-104231-fi8pskod 274 5 the the DT cord-104231-fi8pskod 274 6 same same JJ cord-104231-fi8pskod 274 7 ( ( -LRB- cord-104231-fi8pskod 274 8 0.08 0.08 CD cord-104231-fi8pskod 274 9 + + SYM cord-104231-fi8pskod 274 10 0.05 0.05 CD cord-104231-fi8pskod 274 11 gold gold NN cord-104231-fi8pskod 274 12 particles/#m particles/#m NN cord-104231-fi8pskod 274 13 ) ) -RRB- cord-104231-fi8pskod 274 14 suggesting suggest VBG cord-104231-fi8pskod 274 15 that that IN cord-104231-fi8pskod 274 16 CD4-C CD4-C NNP cord-104231-fi8pskod 274 17 was be VBD cord-104231-fi8pskod 274 18 as as RB cord-104231-fi8pskod 274 19 efficiently efficiently RB cord-104231-fi8pskod 274 20 removed remove VBN cord-104231-fi8pskod 274 21 from from IN cord-104231-fi8pskod 274 22 the the DT cord-104231-fi8pskod 274 23 cell cell NN cord-104231-fi8pskod 274 24 surface surface NN cord-104231-fi8pskod 274 25 and and CC cord-104231-fi8pskod 274 26 delivered deliver VBN cord-104231-fi8pskod 274 27 to to IN cord-104231-fi8pskod 274 28 endosomes endosome NNS cord-104231-fi8pskod 274 29 . . . cord-104231-fi8pskod 275 1 The the DT cord-104231-fi8pskod 275 2 protein protein NN cord-104231-fi8pskod 275 3 was be VBD cord-104231-fi8pskod 275 4 still still RB cord-104231-fi8pskod 275 5 concentrated concentrate VBN cord-104231-fi8pskod 275 6 in in IN cord-104231-fi8pskod 275 7 the the DT cord-104231-fi8pskod 275 8 TGN TGN NNP cord-104231-fi8pskod 275 9 since since IN cord-104231-fi8pskod 275 10 the the DT cord-104231-fi8pskod 275 11 linear linear JJ cord-104231-fi8pskod 275 12 density density NN cord-104231-fi8pskod 275 13 was be VBD cord-104231-fi8pskod 275 14 9 9 CD cord-104231-fi8pskod 275 15 - - SYM cord-104231-fi8pskod 275 16 24 24 CD cord-104231-fi8pskod 275 17 times time NNS cord-104231-fi8pskod 275 18 higher high JJR cord-104231-fi8pskod 275 19 than than IN cord-104231-fi8pskod 275 20 that that DT cord-104231-fi8pskod 275 21 in in IN cord-104231-fi8pskod 275 22 the the DT cord-104231-fi8pskod 275 23 plasma plasma NN cord-104231-fi8pskod 275 24 membrane membrane NN cord-104231-fi8pskod 275 25 ( ( -LRB- cord-104231-fi8pskod 275 26 Table table NN cord-104231-fi8pskod 275 27 I i CD cord-104231-fi8pskod 275 28 ) ) -RRB- cord-104231-fi8pskod 275 29 . . . cord-104231-fi8pskod 276 1 As as IN cord-104231-fi8pskod 276 2 a a DT cord-104231-fi8pskod 276 3 final final JJ cord-104231-fi8pskod 276 4 control control NN cord-104231-fi8pskod 276 5 , , , cord-104231-fi8pskod 276 6 CD4 CD4 NNP cord-104231-fi8pskod 276 7 was be VBD cord-104231-fi8pskod 276 8 also also RB cord-104231-fi8pskod 276 9 expressed express VBN cord-104231-fi8pskod 276 10 transiently transiently RB cord-104231-fi8pskod 276 11 in in IN cord-104231-fi8pskod 276 12 HeLa HeLa NNP cord-104231-fi8pskod 276 13 cells cell NNS cord-104231-fi8pskod 276 14 . . . cord-104231-fi8pskod 277 1 All all DT cord-104231-fi8pskod 277 2 of of IN cord-104231-fi8pskod 277 3 the the DT cord-104231-fi8pskod 277 4 protein protein NN cord-104231-fi8pskod 277 5 was be VBD cord-104231-fi8pskod 277 6 found find VBN cord-104231-fi8pskod 277 7 in in IN cord-104231-fi8pskod 277 8 endosomes endosome NNS cord-104231-fi8pskod 277 9 and and CC cord-104231-fi8pskod 277 10 on on IN cord-104231-fi8pskod 277 11 the the DT cord-104231-fi8pskod 277 12 plasma plasma NN cord-104231-fi8pskod 277 13 membrane membrane NN cord-104231-fi8pskod 277 14 after after IN cord-104231-fi8pskod 277 15 treatment treatment NN cord-104231-fi8pskod 277 16 with with IN cord-104231-fi8pskod 277 17 cycloheximide cycloheximide NN cord-104231-fi8pskod 277 18 and and CC cord-104231-fi8pskod 277 19 in in IN cord-104231-fi8pskod 277 20 agreement agreement NN cord-104231-fi8pskod 277 21 with with IN cord-104231-fi8pskod 277 22 studies study NNS cord-104231-fi8pskod 277 23 carried carry VBN cord-104231-fi8pskod 277 24 out out RP cord-104231-fi8pskod 277 25 by by IN cord-104231-fi8pskod 277 26 Pelchen Pelchen NNP cord-104231-fi8pskod 277 27 - - HYPH cord-104231-fi8pskod 277 28 Matthews Matthews NNP cord-104231-fi8pskod 277 29 et et NNP cord-104231-fi8pskod 277 30 al al NNP cord-104231-fi8pskod 277 31 . . . cord-104231-fi8pskod 278 1 ( ( -LRB- cord-104231-fi8pskod 278 2 1991 1991 CD cord-104231-fi8pskod 278 3 ) ) -RRB- cord-104231-fi8pskod 278 4 . . . cord-104231-fi8pskod 279 1 Little little JJ cord-104231-fi8pskod 279 2 if if IN cord-104231-fi8pskod 279 3 any any DT cord-104231-fi8pskod 279 4 was be VBD cord-104231-fi8pskod 279 5 found find VBN cord-104231-fi8pskod 279 6 in in IN cord-104231-fi8pskod 279 7 the the DT cord-104231-fi8pskod 279 8 TGN TGN NNP cord-104231-fi8pskod 279 9 ( ( -LRB- cord-104231-fi8pskod 279 10 data datum NNS cord-104231-fi8pskod 279 11 not not RB cord-104231-fi8pskod 279 12 shown show VBN cord-104231-fi8pskod 279 13 ) ) -RRB- cord-104231-fi8pskod 279 14 . . . cord-104231-fi8pskod 280 1 This this DT cord-104231-fi8pskod 280 2 shows show VBZ cord-104231-fi8pskod 280 3 that that IN cord-104231-fi8pskod 280 4 accumulation accumulation NN cord-104231-fi8pskod 280 5 of of IN cord-104231-fi8pskod 280 6 the the DT cord-104231-fi8pskod 280 7 hybrid hybrid JJ cord-104231-fi8pskod 280 8 proteins protein NNS cord-104231-fi8pskod 280 9 in in IN cord-104231-fi8pskod 280 10 the the DT cord-104231-fi8pskod 280 11 TGN TGN NNP cord-104231-fi8pskod 280 12 was be VBD cord-104231-fi8pskod 280 13 not not RB cord-104231-fi8pskod 280 14 a a DT cord-104231-fi8pskod 280 15 consequence consequence NN cord-104231-fi8pskod 280 16 of of IN cord-104231-fi8pskod 280 17 the the DT cord-104231-fi8pskod 280 18 CD4 CD4 NNP cord-104231-fi8pskod 280 19 ectodomain ectodomain NNP cord-104231-fi8pskod 280 20 . . . cord-104231-fi8pskod 281 1 FACS FACS NNP cord-104231-fi8pskod 281 2 analysis analysis NN cord-104231-fi8pskod 281 3 was be VBD cord-104231-fi8pskod 281 4 used use VBN cord-104231-fi8pskod 281 5 to to TO cord-104231-fi8pskod 281 6 determine determine VB cord-104231-fi8pskod 281 7 the the DT cord-104231-fi8pskod 281 8 amount amount NN cord-104231-fi8pskod 281 9 of of IN cord-104231-fi8pskod 281 10 CD8 CD8 NNP cord-104231-fi8pskod 281 11 and and CC cord-104231-fi8pskod 281 12 CD8 CD8 NNP cord-104231-fi8pskod 281 13 hybrid hybrid NN cord-104231-fi8pskod 281 14 proteins protein NNS cord-104231-fi8pskod 281 15 on on IN cord-104231-fi8pskod 281 16 the the DT cord-104231-fi8pskod 281 17 cell cell NN cord-104231-fi8pskod 281 18 surface surface NN cord-104231-fi8pskod 281 19 . . . cord-104231-fi8pskod 282 1 Fixed fix VBN cord-104231-fi8pskod 282 2 , , , cord-104231-fi8pskod 282 3 nonpermeabilized nonpermeabilized JJ cord-104231-fi8pskod 282 4 cells cell NNS cord-104231-fi8pskod 282 5 transfected transfecte VBN cord-104231-fi8pskod 282 6 with with IN cord-104231-fi8pskod 282 7 the the DT cord-104231-fi8pskod 282 8 appropriate appropriate JJ cord-104231-fi8pskod 283 1 eDNA eDNA NNP cord-104231-fi8pskod 283 2 were be VBD cord-104231-fi8pskod 283 3 stained stain VBN cord-104231-fi8pskod 283 4 with with IN cord-104231-fi8pskod 283 5 the the DT cord-104231-fi8pskod 283 6 OKT8 OKT8 NNP cord-104231-fi8pskod 283 7 antibody antibody NN cord-104231-fi8pskod 283 8 to to IN cord-104231-fi8pskod 283 9 CD8 CD8 NNP cord-104231-fi8pskod 283 10 and and CC cord-104231-fi8pskod 283 11 visualized visualize VBD cord-104231-fi8pskod 283 12 using use VBG cord-104231-fi8pskod 283 13 a a DT cord-104231-fi8pskod 283 14 secondary secondary JJ cord-104231-fi8pskod 283 15 antibody antibody NN cord-104231-fi8pskod 283 16 conjugated conjugate VBN cord-104231-fi8pskod 283 17 to to IN cord-104231-fi8pskod 283 18 FITC FITC NNP cord-104231-fi8pskod 284 1 ( ( -LRB- cord-104231-fi8pskod 284 2 Fig Fig NNP cord-104231-fi8pskod 284 3 . . NNP cord-104231-fi8pskod 284 4 7 7 CD cord-104231-fi8pskod 284 5 ) ) -RRB- cord-104231-fi8pskod 284 6 . . . cord-104231-fi8pskod 285 1 Mock mock JJ cord-104231-fi8pskod 285 2 - - HYPH cord-104231-fi8pskod 285 3 transfected transfecte VBN cord-104231-fi8pskod 285 4 cells cell NNS cord-104231-fi8pskod 285 5 were be VBD cord-104231-fi8pskod 285 6 used use VBN cord-104231-fi8pskod 285 7 as as IN cord-104231-fi8pskod 285 8 the the DT cord-104231-fi8pskod 285 9 negative negative JJ cord-104231-fi8pskod 285 10 control control NN cord-104231-fi8pskod 285 11 and and CC cord-104231-fi8pskod 285 12 the the DT cord-104231-fi8pskod 285 13 gate gate NN cord-104231-fi8pskod 285 14 was be VBD cord-104231-fi8pskod 285 15 set set VBN cord-104231-fi8pskod 285 16 such such JJ cord-104231-fi8pskod 285 17 that that IN cord-104231-fi8pskod 285 18 1 1 CD cord-104231-fi8pskod 285 19 % % NN cord-104231-fi8pskod 285 20 of of IN cord-104231-fi8pskod 285 21 these these DT cord-104231-fi8pskod 285 22 cells cell NNS cord-104231-fi8pskod 285 23 scored score VBD cord-104231-fi8pskod 285 24 positive positive JJ cord-104231-fi8pskod 285 25 . . . cord-104231-fi8pskod 286 1 The the DT cord-104231-fi8pskod 286 2 profile profile NN cord-104231-fi8pskod 286 3 of of IN cord-104231-fi8pskod 286 4 CD8 CD8 NNP cord-104231-fi8pskod 286 5 expression expression NN cord-104231-fi8pskod 286 6 was be VBD cord-104231-fi8pskod 286 7 characteristic characteristic JJ cord-104231-fi8pskod 286 8 of of IN cord-104231-fi8pskod 286 9 that that DT cord-104231-fi8pskod 286 10 in in IN cord-104231-fi8pskod 286 11 a a DT cord-104231-fi8pskod 286 12 population population NN cord-104231-fi8pskod 286 13 of of IN cord-104231-fi8pskod 286 14 transiently transiently RB cord-104231-fi8pskod 286 15 transfected transfecte VBN cord-104231-fi8pskod 286 16 cells cell NNS cord-104231-fi8pskod 286 17 ( ( -LRB- cord-104231-fi8pskod 286 18 Nilsson Nilsson NNP cord-104231-fi8pskod 286 19 et et NNP cord-104231-fi8pskod 286 20 al al NNP cord-104231-fi8pskod 286 21 . . NNP cord-104231-fi8pskod 286 22 , , , cord-104231-fi8pskod 286 23 1989 1989 CD cord-104231-fi8pskod 286 24 ; ; : cord-104231-fi8pskod 287 1 Jackson Jackson NNP cord-104231-fi8pskod 287 2 et et NNP cord-104231-fi8pskod 287 3 al al NNP cord-104231-fi8pskod 287 4 . . NNP cord-104231-fi8pskod 287 5 , , , cord-104231-fi8pskod 287 6 1990 1990 CD cord-104231-fi8pskod 287 7 ) ) -RRB- cord-104231-fi8pskod 287 8 and and CC cord-104231-fi8pskod 287 9 58 58 CD cord-104231-fi8pskod 287 10 % % NN cord-104231-fi8pskod 287 11 scored score VBD cord-104231-fi8pskod 287 12 positive positive JJ cord-104231-fi8pskod 287 13 ( ( -LRB- cord-104231-fi8pskod 287 14 Fig fig NN cord-104231-fi8pskod 287 15 . . . cord-104231-fi8pskod 287 16 _SP cord-104231-fi8pskod 287 17 7 7 CD cord-104231-fi8pskod 287 18 A A NNP cord-104231-fi8pskod 287 19 , , , cord-104231-fi8pskod 287 20 Table Table NNP cord-104231-fi8pskod 287 21 1I 1i CD cord-104231-fi8pskod 287 22 ) ) -RRB- cord-104231-fi8pskod 287 23 . . . cord-104231-fi8pskod 288 1 When when WRB cord-104231-fi8pskod 288 2 both both DT cord-104231-fi8pskod 288 3 localization localization NN cord-104231-fi8pskod 288 4 signals signal NNS cord-104231-fi8pskod 288 5 were be VBD cord-104231-fi8pskod 288 6 present present JJ cord-104231-fi8pskod 288 7 ( ( -LRB- cord-104231-fi8pskod 288 8 CDS CDS NNP cord-104231-fi8pskod 288 9 - - HYPH cord-104231-fi8pskod 288 10 MC MC NNP cord-104231-fi8pskod 288 11 ) ) -RRB- cord-104231-fi8pskod 289 1 only only RB cord-104231-fi8pskod 289 2 6 6 CD cord-104231-fi8pskod 289 3 % % NN cord-104231-fi8pskod 289 4 of of IN cord-104231-fi8pskod 289 5 the the DT cord-104231-fi8pskod 289 6 cells cell NNS cord-104231-fi8pskod 289 7 showed show VBD cord-104231-fi8pskod 289 8 surface surface NN cord-104231-fi8pskod 289 9 staining staining NN cord-104231-fi8pskod 289 10 ( ( -LRB- cord-104231-fi8pskod 290 1 Fig Fig NNP cord-104231-fi8pskod 290 2 . . NNP cord-104231-fi8pskod 290 3 7 7 CD cord-104231-fi8pskod 290 4 B b NN cord-104231-fi8pskod 290 5 , , , cord-104231-fi8pskod 290 6 Table table NN cord-104231-fi8pskod 290 7 ] ] -RRB- cord-104231-fi8pskod 290 8 I i NN cord-104231-fi8pskod 290 9 ) ) -RRB- cord-104231-fi8pskod 291 1 and and CC cord-104231-fi8pskod 291 2 this this DT cord-104231-fi8pskod 291 3 rose rise VBD cord-104231-fi8pskod 291 4 to to IN cord-104231-fi8pskod 291 5 19 19 CD cord-104231-fi8pskod 291 6 % % NN cord-104231-fi8pskod 291 7 when when WRB cord-104231-fi8pskod 291 8 the the DT cord-104231-fi8pskod 291 9 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 291 10 signal signal NN cord-104231-fi8pskod 291 11 was be VBD cord-104231-fi8pskod 291 12 deleted delete VBN cord-104231-fi8pskod 291 13 ( ( -LRB- cord-104231-fi8pskod 291 14 CD8-MAC CD8-MAC NNP cord-104231-fi8pskod 291 15 ) ) -RRB- cord-104231-fi8pskod 292 1 ( ( -LRB- cord-104231-fi8pskod 292 2 Fig Fig NNP cord-104231-fi8pskod 292 3 . . . cord-104231-fi8pskod 293 1 7 7 CD cord-104231-fi8pskod 293 2 D d NN cord-104231-fi8pskod 293 3 , , , cord-104231-fi8pskod 293 4 Table Table NNP cord-104231-fi8pskod 293 5 1I 1i CD cord-104231-fi8pskod 293 6 ) ) -RRB- cord-104231-fi8pskod 293 7 suggesting suggest VBG cord-104231-fi8pskod 293 8 that that IN cord-104231-fi8pskod 293 9 the the DT cord-104231-fi8pskod 293 10 membrane membrane NN cord-104231-fi8pskod 293 11 - - HYPH cord-104231-fi8pskod 293 12 spanning span VBG cord-104231-fi8pskod 293 13 signal signal NN cord-104231-fi8pskod 293 14 alone alone RB cord-104231-fi8pskod 293 15 could could MD cord-104231-fi8pskod 293 16 retain retain VB cord-104231-fi8pskod 293 17 the the DT cord-104231-fi8pskod 293 18 hybrid hybrid JJ cord-104231-fi8pskod 293 19 protein protein NN cord-104231-fi8pskod 293 20 . . . cord-104231-fi8pskod 294 1 This this DT cord-104231-fi8pskod 294 2 was be VBD cord-104231-fi8pskod 294 3 confirmed confirm VBN cord-104231-fi8pskod 294 4 by by IN cord-104231-fi8pskod 294 5 expression expression NN cord-104231-fi8pskod 294 6 of of IN cord-104231-fi8pskod 294 7 CD8-M. CD8-M. NNP cord-104231-fi8pskod 294 8 Only only RB cord-104231-fi8pskod 294 9 13 13 CD cord-104231-fi8pskod 294 10 % % NN cord-104231-fi8pskod 294 11 of of IN cord-104231-fi8pskod 294 12 the the DT cord-104231-fi8pskod 294 13 cells cell NNS cord-104231-fi8pskod 294 14 scored score VBD cord-104231-fi8pskod 294 15 positive positive JJ cord-104231-fi8pskod 294 16 ( ( -LRB- cord-104231-fi8pskod 294 17 Fig Fig NNP cord-104231-fi8pskod 294 18 . . . cord-104231-fi8pskod 294 19 7 7 CD cord-104231-fi8pskod 294 20 C c NN cord-104231-fi8pskod 294 21 , , , cord-104231-fi8pskod 294 22 Table Table NNP cord-104231-fi8pskod 294 23 II II NNP cord-104231-fi8pskod 294 24 ) ) -RRB- cord-104231-fi8pskod 294 25 . . . cord-104231-fi8pskod 295 1 The the DT cord-104231-fi8pskod 295 2 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 295 3 signal signal NN cord-104231-fi8pskod 295 4 alone alone RB cord-104231-fi8pskod 295 5 could could MD cord-104231-fi8pskod 295 6 also also RB cord-104231-fi8pskod 295 7 retain retain VB cord-104231-fi8pskod 295 8 the the DT cord-104231-fi8pskod 295 9 hybrid hybrid JJ cord-104231-fi8pskod 295 10 protein protein NN cord-104231-fi8pskod 295 11 . . . cord-104231-fi8pskod 296 1 Only only RB cord-104231-fi8pskod 296 2 . . . cord-104231-fi8pskod 297 1 Surface surface NN cord-104231-fi8pskod 297 2 expression expression NN cord-104231-fi8pskod 297 3 of of IN cord-104231-fi8pskod 297 4 CD8 CD8 NNP cord-104231-fi8pskod 297 5 and and CC cord-104231-fi8pskod 297 6 CD8 CD8 NNP cord-104231-fi8pskod 297 7 hybrid hybrid NN cord-104231-fi8pskod 297 8 proteins protein NNS cord-104231-fi8pskod 297 9 analyzed analyze VBD cord-104231-fi8pskod 297 10 using use VBG cord-104231-fi8pskod 297 11 FACS FACS NNP cord-104231-fi8pskod 297 12 analysis analysis NN cord-104231-fi8pskod 297 13 . . . cord-104231-fi8pskod 298 1 HeLa HeLa NNP cord-104231-fi8pskod 298 2 cells cell NNS cord-104231-fi8pskod 298 3 were be VBD cord-104231-fi8pskod 298 4 fixed fix VBN cord-104231-fi8pskod 298 5 and and CC cord-104231-fi8pskod 298 6 stained stain VBN cord-104231-fi8pskod 298 7 with with IN cord-104231-fi8pskod 298 8 monoclonal monoclonal JJ cord-104231-fi8pskod 298 9 antibody antibody NN cord-104231-fi8pskod 298 10 to to IN cord-104231-fi8pskod 298 11 CD8 CD8 NNP cord-104231-fi8pskod 298 12 and and CC cord-104231-fi8pskod 298 13 visualized visualize VBD cord-104231-fi8pskod 298 14 using use VBG cord-104231-fi8pskod 298 15 a a DT cord-104231-fi8pskod 298 16 secondary secondary JJ cord-104231-fi8pskod 298 17 antibody antibody NN cord-104231-fi8pskod 298 18 conjugated conjugate VBN cord-104231-fi8pskod 298 19 to to IN cord-104231-fi8pskod 298 20 FITC FITC NNP cord-104231-fi8pskod 298 21 . . . cord-104231-fi8pskod 299 1 The the DT cord-104231-fi8pskod 299 2 gate gate NN cord-104231-fi8pskod 299 3 on on IN cord-104231-fi8pskod 299 4 the the DT cord-104231-fi8pskod 299 5 x x NN cord-104231-fi8pskod 299 6 - - HYPH cord-104231-fi8pskod 299 7 axis axis NN cord-104231-fi8pskod 299 8 ( ( -LRB- cord-104231-fi8pskod 299 9 fluorescence fluorescence NN cord-104231-fi8pskod 299 10 units unit NNS cord-104231-fi8pskod 299 11 ) ) -RRB- cord-104231-fi8pskod 299 12 was be VBD cord-104231-fi8pskod 299 13 set set VBN cord-104231-fi8pskod 299 14 using use VBG cord-104231-fi8pskod 299 15 mock mock NN cord-104231-fi8pskod 299 16 - - HYPH cord-104231-fi8pskod 299 17 transfected transfecte VBN cord-104231-fi8pskod 299 18 cells cell NNS cord-104231-fi8pskod 299 19 as as IN cord-104231-fi8pskod 299 20 a a DT cord-104231-fi8pskod 299 21 negative negative JJ cord-104231-fi8pskod 299 22 control control NN cord-104231-fi8pskod 299 23 . . . cord-104231-fi8pskod 300 1 Cells cell NNS cord-104231-fi8pskod 300 2 scoring score VBG cord-104231-fi8pskod 300 3 to to IN cord-104231-fi8pskod 300 4 the the DT cord-104231-fi8pskod 300 5 right right NN cord-104231-fi8pskod 300 6 of of IN cord-104231-fi8pskod 300 7 this this DT cord-104231-fi8pskod 300 8 point point NN cord-104231-fi8pskod 300 9 were be VBD cord-104231-fi8pskod 300 10 regarded regard VBN cord-104231-fi8pskod 300 11 as as IN cord-104231-fi8pskod 300 12 positive positive JJ cord-104231-fi8pskod 300 13 for for IN cord-104231-fi8pskod 300 14 cell cell NN cord-104231-fi8pskod 300 15 surface surface NN cord-104231-fi8pskod 300 16 fluorescence fluorescence NN cord-104231-fi8pskod 300 17 ( ( -LRB- cord-104231-fi8pskod 300 18 see see VB cord-104231-fi8pskod 300 19 Table table NN cord-104231-fi8pskod 300 20 I1 i1 NN cord-104231-fi8pskod 300 21 ) ) -RRB- cord-104231-fi8pskod 300 22 . . . cord-104231-fi8pskod 301 1 * * NFP cord-104231-fi8pskod 301 2 The the DT cord-104231-fi8pskod 301 3 gate gate NN cord-104231-fi8pskod 301 4 was be VBD cord-104231-fi8pskod 301 5 set set VBN cord-104231-fi8pskod 301 6 using use VBG cord-104231-fi8pskod 301 7 moek moek VBN cord-104231-fi8pskod 301 8 - - HYPH cord-104231-fi8pskod 301 9 transfeeted transfeete VBN cord-104231-fi8pskod 301 10 cells cell NNS cord-104231-fi8pskod 301 11 and and CC cord-104231-fi8pskod 301 12 1 1 CD cord-104231-fi8pskod 301 13 % % NN cord-104231-fi8pskod 301 14 were be VBD cord-104231-fi8pskod 301 15 positive positive JJ cord-104231-fi8pskod 301 16 . . . cord-104231-fi8pskod 302 1 5,000 5,000 CD cord-104231-fi8pskod 302 2 cells cell NNS cord-104231-fi8pskod 302 3 were be VBD cord-104231-fi8pskod 302 4 analyzed analyze VBN cord-104231-fi8pskod 302 5 in in IN cord-104231-fi8pskod 302 6 each each DT cord-104231-fi8pskod 302 7 experiment experiment NN cord-104231-fi8pskod 302 8 . . . cord-104231-fi8pskod 303 1 * * NFP cord-104231-fi8pskod 303 2 The the DT cord-104231-fi8pskod 303 3 total total JJ cord-104231-fi8pskod 303 4 fluorescence fluorescence NN cord-104231-fi8pskod 303 5 intensity intensity NN cord-104231-fi8pskod 303 6 was be VBD cord-104231-fi8pskod 303 7 calculated calculate VBN cord-104231-fi8pskod 303 8 by by IN cord-104231-fi8pskod 303 9 integrating integrate VBG cord-104231-fi8pskod 303 10 the the DT cord-104231-fi8pskod 303 11 area area NN cord-104231-fi8pskod 303 12 under under IN cord-104231-fi8pskod 303 13 the the DT cord-104231-fi8pskod 303 14 positive positive JJ cord-104231-fi8pskod 303 15 cells cell NNS cord-104231-fi8pskod 303 16 and and CC cord-104231-fi8pskod 303 17 expressing express VBG cord-104231-fi8pskod 303 18 the the DT cord-104231-fi8pskod 303 19 results result NNS cord-104231-fi8pskod 303 20 as as IN cord-104231-fi8pskod 303 21 a a DT cord-104231-fi8pskod 303 22 percentage percentage NN cord-104231-fi8pskod 303 23 of of IN cord-104231-fi8pskod 303 24 the the DT cord-104231-fi8pskod 303 25 value value NN cord-104231-fi8pskod 303 26 for for IN cord-104231-fi8pskod 303 27 CD8 CD8 NNP cord-104231-fi8pskod 303 28 . . . cord-104231-fi8pskod 304 1 ( ( -LRB- cord-104231-fi8pskod 304 2 Fig Fig NNP cord-104231-fi8pskod 304 3 . . . cord-104231-fi8pskod 304 4 7 7 CD cord-104231-fi8pskod 304 5 E e NN cord-104231-fi8pskod 304 6 , , , cord-104231-fi8pskod 304 7 Table Table NNP cord-104231-fi8pskod 304 8 II II NNP cord-104231-fi8pskod 304 9 ) ) -RRB- cord-104231-fi8pskod 304 10 . . . cord-104231-fi8pskod 305 1 Removal removal NN cord-104231-fi8pskod 305 2 of of IN cord-104231-fi8pskod 305 3 the the DT cord-104231-fi8pskod 305 4 tyrosine tyrosine NN cord-104231-fi8pskod 305 5 - - HYPH cord-104231-fi8pskod 305 6 based base VBN cord-104231-fi8pskod 305 7 motif motif NN cord-104231-fi8pskod 305 8 in in IN cord-104231-fi8pskod 305 9 this this DT cord-104231-fi8pskod 305 10 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 305 11 signal signal NN cord-104231-fi8pskod 305 12 destroyed destroy VBD cord-104231-fi8pskod 305 13 its -PRON- PRP$ cord-104231-fi8pskod 305 14 ability ability NN cord-104231-fi8pskod 305 15 to to TO cord-104231-fi8pskod 305 16 retain retain VB cord-104231-fi8pskod 305 17 hybrid hybrid JJ cord-104231-fi8pskod 305 18 proteins protein NNS cord-104231-fi8pskod 305 19 . . . cord-104231-fi8pskod 306 1 More More JJR cord-104231-fi8pskod 306 2 than than IN cord-104231-fi8pskod 306 3 67 67 CD cord-104231-fi8pskod 306 4 % % NN cord-104231-fi8pskod 306 5 of of IN cord-104231-fi8pskod 306 6 cells cell NNS cord-104231-fi8pskod 306 7 expressing express VBG cord-104231-fi8pskod 306 8 CD8-AC cd8-ac NN cord-104231-fi8pskod 306 9 scored score VBD cord-104231-fi8pskod 306 10 positive positive JJ cord-104231-fi8pskod 306 11 ( ( -LRB- cord-104231-fi8pskod 306 12 Fig Fig NNP cord-104231-fi8pskod 306 13 . . . cord-104231-fi8pskod 306 14 7 7 CD cord-104231-fi8pskod 306 15 F f NN cord-104231-fi8pskod 306 16 , , , cord-104231-fi8pskod 306 17 , , , cord-104231-fi8pskod 306 18 Table Table NNP cord-104231-fi8pskod 306 19 II II NNP cord-104231-fi8pskod 306 20 ) ) -RRB- cord-104231-fi8pskod 306 21 , , , cord-104231-fi8pskod 306 22 slightly slightly RB cord-104231-fi8pskod 306 23 higher high JJR cord-104231-fi8pskod 306 24 than than IN cord-104231-fi8pskod 306 25 the the DT cord-104231-fi8pskod 306 26 number number NN cord-104231-fi8pskod 306 27 expressing express VBG cord-104231-fi8pskod 306 28 CD8 CD8 NNP cord-104231-fi8pskod 306 29 alone alone RB cord-104231-fi8pskod 306 30 ( ( -LRB- cord-104231-fi8pskod 306 31 58 58 CD cord-104231-fi8pskod 306 32 % % NN cord-104231-fi8pskod 306 33 , , , cord-104231-fi8pskod 306 34 Fig Fig NNP cord-104231-fi8pskod 306 35 . . . cord-104231-fi8pskod 307 1 7 7 CD cord-104231-fi8pskod 307 2 A a NN cord-104231-fi8pskod 307 3 , , , cord-104231-fi8pskod 307 4 Table Table NNP cord-104231-fi8pskod 307 5 II II NNP cord-104231-fi8pskod 307 6 ) ) -RRB- cord-104231-fi8pskod 307 7 . . . cord-104231-fi8pskod 308 1 The the DT cord-104231-fi8pskod 308 2 total total JJ cord-104231-fi8pskod 308 3 fluorescence fluorescence NN cord-104231-fi8pskod 308 4 of of IN cord-104231-fi8pskod 308 5 positive positive JJ cord-104231-fi8pskod 308 6 cells cell NNS cord-104231-fi8pskod 308 7 for for IN cord-104231-fi8pskod 308 8 each each DT cord-104231-fi8pskod 308 9 protein protein NN cord-104231-fi8pskod 308 10 was be VBD cord-104231-fi8pskod 308 11 also also RB cord-104231-fi8pskod 308 12 calculated calculate VBN cord-104231-fi8pskod 308 13 by by IN cord-104231-fi8pskod 308 14 integration integration NN cord-104231-fi8pskod 308 15 of of IN cord-104231-fi8pskod 308 16 the the DT cord-104231-fi8pskod 308 17 results result NNS cord-104231-fi8pskod 308 18 presented present VBN cord-104231-fi8pskod 308 19 in in IN cord-104231-fi8pskod 308 20 Fig Fig NNP cord-104231-fi8pskod 308 21 . . . cord-104231-fi8pskod 309 1 7 7 CD cord-104231-fi8pskod 309 2 . . . cord-104231-fi8pskod 310 1 These these DT cord-104231-fi8pskod 310 2 calculations calculation NNS cord-104231-fi8pskod 310 3 , , , cord-104231-fi8pskod 310 4 presented present VBN cord-104231-fi8pskod 310 5 in in IN cord-104231-fi8pskod 310 6 Table Table NNP cord-104231-fi8pskod 310 7 II II NNP cord-104231-fi8pskod 310 8 , , , cord-104231-fi8pskod 310 9 are be VBP cord-104231-fi8pskod 310 10 in in IN cord-104231-fi8pskod 310 11 close close JJ cord-104231-fi8pskod 310 12 agreement agreement NN cord-104231-fi8pskod 310 13 with with IN cord-104231-fi8pskod 310 14 the the DT cord-104231-fi8pskod 310 15 percentage percentage NN cord-104231-fi8pskod 310 16 of of IN cord-104231-fi8pskod 310 17 cells cell NNS cord-104231-fi8pskod 310 18 scoring score VBG cord-104231-fi8pskod 310 19 positive positive JJ cord-104231-fi8pskod 310 20 and and CC cord-104231-fi8pskod 310 21 provide provide VB cord-104231-fi8pskod 310 22 further further JJ cord-104231-fi8pskod 310 23 evidence evidence NN cord-104231-fi8pskod 310 24 that that IN cord-104231-fi8pskod 310 25 both both CC cord-104231-fi8pskod 310 26 the the DT cord-104231-fi8pskod 310 27 membrane membrane NN cord-104231-fi8pskod 310 28 - - HYPH cord-104231-fi8pskod 310 29 spanning span VBG cord-104231-fi8pskod 310 30 and and CC cord-104231-fi8pskod 310 31 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 310 32 signals signal NNS cord-104231-fi8pskod 310 33 can can MD cord-104231-fi8pskod 310 34 , , , cord-104231-fi8pskod 310 35 independently independently RB cord-104231-fi8pskod 310 36 , , , cord-104231-fi8pskod 310 37 retain retain VB cord-104231-fi8pskod 310 38 hybrid hybrid JJ cord-104231-fi8pskod 310 39 proteins protein NNS cord-104231-fi8pskod 310 40 inside inside IN cord-104231-fi8pskod 310 41 the the DT cord-104231-fi8pskod 310 42 cell cell NN cord-104231-fi8pskod 310 43 . . . cord-104231-fi8pskod 311 1 These these DT cord-104231-fi8pskod 311 2 data datum NNS cord-104231-fi8pskod 311 3 were be VBD cord-104231-fi8pskod 311 4 also also RB cord-104231-fi8pskod 311 5 supported support VBN cord-104231-fi8pskod 311 6 by by IN cord-104231-fi8pskod 311 7 immunofluorescence immunofluorescence NN cord-104231-fi8pskod 311 8 microscopy microscopy NN cord-104231-fi8pskod 311 9 on on IN cord-104231-fi8pskod 311 10 non non JJ cord-104231-fi8pskod 311 11 - - JJ cord-104231-fi8pskod 311 12 permeabilized permeabilized JJ cord-104231-fi8pskod 311 13 cells cell NNS cord-104231-fi8pskod 311 14 ( ( -LRB- cord-104231-fi8pskod 311 15 data datum NNS cord-104231-fi8pskod 311 16 not not RB cord-104231-fi8pskod 311 17 shown show VBN cord-104231-fi8pskod 311 18 ) ) -RRB- cord-104231-fi8pskod 311 19 . . . cord-104231-fi8pskod 312 1 There there EX cord-104231-fi8pskod 312 2 was be VBD cord-104231-fi8pskod 312 3 no no DT cord-104231-fi8pskod 312 4 surface surface NN cord-104231-fi8pskod 312 5 staining staining NN cord-104231-fi8pskod 312 6 of of IN cord-104231-fi8pskod 312 7 cells cell NNS cord-104231-fi8pskod 312 8 expressing express VBG cord-104231-fi8pskod 312 9 CD8-MC cd8-mc NN cord-104231-fi8pskod 312 10 or or CC cord-104231-fi8pskod 312 11 CD8-C. CD8-C. NNP cord-104231-fi8pskod 313 1 A a DT cord-104231-fi8pskod 313 2 few few JJ cord-104231-fi8pskod 313 3 of of IN cord-104231-fi8pskod 313 4 the the DT cord-104231-fi8pskod 313 5 transfected transfecte VBN cord-104231-fi8pskod 313 6 cells cell NNS cord-104231-fi8pskod 313 7 expressing express VBG cord-104231-fi8pskod 313 8 CD8-M CD8-M NNP cord-104231-fi8pskod 313 9 showed show VBD cord-104231-fi8pskod 313 10 low low JJ cord-104231-fi8pskod 313 11 levels level NNS cord-104231-fi8pskod 313 12 of of IN cord-104231-fi8pskod 313 13 cell cell NN cord-104231-fi8pskod 313 14 surface surface NN cord-104231-fi8pskod 313 15 staining staining NN cord-104231-fi8pskod 313 16 . . . cord-104231-fi8pskod 314 1 Cells cell NNS cord-104231-fi8pskod 314 2 expressing express VBG cord-104231-fi8pskod 314 3 CD8-AC cd8-ac NN cord-104231-fi8pskod 314 4 showed show VBD cord-104231-fi8pskod 314 5 high high JJ cord-104231-fi8pskod 314 6 levels level NNS cord-104231-fi8pskod 314 7 of of IN cord-104231-fi8pskod 314 8 cell cell NN cord-104231-fi8pskod 314 9 surface surface NN cord-104231-fi8pskod 314 10 staining staining NN cord-104231-fi8pskod 314 11 , , , cord-104231-fi8pskod 314 12 in in IN cord-104231-fi8pskod 314 13 agreement agreement NN cord-104231-fi8pskod 314 14 with with IN cord-104231-fi8pskod 314 15 the the DT cord-104231-fi8pskod 314 16 FACS FACS NNP cord-104231-fi8pskod 314 17 analysis analysis NN cord-104231-fi8pskod 314 18 . . . cord-104231-fi8pskod 315 1 CD4 CD4 NNP cord-104231-fi8pskod 315 2 contains contain VBZ cord-104231-fi8pskod 315 3 two two CD cord-104231-fi8pskod 315 4 N n CD cord-104231-fi8pskod 315 5 - - HYPH cord-104231-fi8pskod 315 6 linked link VBN cord-104231-fi8pskod 315 7 glycosylation glycosylation NN cord-104231-fi8pskod 315 8 sites site NNS cord-104231-fi8pskod 315 9 ( ( -LRB- cord-104231-fi8pskod 315 10 Maddon Maddon NNP cord-104231-fi8pskod 315 11 et et NNP cord-104231-fi8pskod 315 12 al al NNP cord-104231-fi8pskod 315 13 . . NNP cord-104231-fi8pskod 315 14 , , , cord-104231-fi8pskod 315 15 1985 1985 CD cord-104231-fi8pskod 315 16 ) ) -RRB- cord-104231-fi8pskod 315 17 one one CD cord-104231-fi8pskod 315 18 of of IN cord-104231-fi8pskod 315 19 which which WDT cord-104231-fi8pskod 315 20 is be VBZ cord-104231-fi8pskod 315 21 completely completely RB cord-104231-fi8pskod 315 22 processed process VBN cord-104231-fi8pskod 315 23 to to IN cord-104231-fi8pskod 315 24 the the DT cord-104231-fi8pskod 315 25 complex complex JJ cord-104231-fi8pskod 315 26 bi bi JJ cord-104231-fi8pskod 315 27 - - JJ cord-104231-fi8pskod 315 28 antennary antennary JJ cord-104231-fi8pskod 315 29 sialylated sialylated JJ cord-104231-fi8pskod 315 30 structure structure NN cord-104231-fi8pskod 315 31 . . . cord-104231-fi8pskod 316 1 Terminal terminal JJ cord-104231-fi8pskod 316 2 sialic sialic NN cord-104231-fi8pskod 316 3 acid acid NN cord-104231-fi8pskod 316 4 residues residue NNS cord-104231-fi8pskod 316 5 are be VBP cord-104231-fi8pskod 316 6 added add VBN cord-104231-fi8pskod 316 7 by by IN cord-104231-fi8pskod 316 8 ot2,6-sialyltransferase ot2,6-sialyltransferase NNS cord-104231-fi8pskod 316 9 which which WDT cord-104231-fi8pskod 316 10 is be VBZ cord-104231-fi8pskod 316 11 located locate VBN cord-104231-fi8pskod 316 12 in in IN cord-104231-fi8pskod 316 13 the the DT cord-104231-fi8pskod 316 14 trans trans NNP cord-104231-fi8pskod 316 15 - - NNP cord-104231-fi8pskod 316 16 Golgi Golgi NNP cord-104231-fi8pskod 316 17 cisterna cisterna NNP cord-104231-fi8pskod 316 18 and and CC cord-104231-fi8pskod 316 19 the the DT cord-104231-fi8pskod 316 20 TGN TGN NNP cord-104231-fi8pskod 316 21 ( ( -LRB- cord-104231-fi8pskod 316 22 Roth Roth NNP cord-104231-fi8pskod 316 23 et et FW cord-104231-fi8pskod 316 24 al al NNP cord-104231-fi8pskod 316 25 . . NNP cord-104231-fi8pskod 316 26 , , , cord-104231-fi8pskod 316 27 1985 1985 CD cord-104231-fi8pskod 316 28 ) ) -RRB- cord-104231-fi8pskod 316 29 . . . cord-104231-fi8pskod 317 1 Neuraminidase Neuraminidase NNP cord-104231-fi8pskod 317 2 cleaves cleave VBZ cord-104231-fi8pskod 317 3 off off RP cord-104231-fi8pskod 317 4 these these DT cord-104231-fi8pskod 317 5 sialic sialic JJ cord-104231-fi8pskod 317 6 acid acid NN cord-104231-fi8pskod 317 7 residues residue NNS cord-104231-fi8pskod 317 8 producing produce VBG cord-104231-fi8pskod 317 9 a a DT cord-104231-fi8pskod 317 10 small small JJ cord-104231-fi8pskod 317 11 mobility mobility NN cord-104231-fi8pskod 317 12 shift shift NN cord-104231-fi8pskod 317 13 on on IN cord-104231-fi8pskod 317 14 SDS SDS NNP cord-104231-fi8pskod 317 15 - - HYPH cord-104231-fi8pskod 317 16 PAGE PAGE NNP cord-104231-fi8pskod 317 17 ( ( -LRB- cord-104231-fi8pskod 317 18 Nilsson Nilsson NNP cord-104231-fi8pskod 317 19 et et NNP cord-104231-fi8pskod 317 20 al al NNP cord-104231-fi8pskod 317 21 . . NNP cord-104231-fi8pskod 317 22 , , , cord-104231-fi8pskod 317 23 1989 1989 CD cord-104231-fi8pskod 317 24 ; ; : cord-104231-fi8pskod 317 25 Jackson Jackson NNP cord-104231-fi8pskod 317 26 et et NNP cord-104231-fi8pskod 317 27 al al NNP cord-104231-fi8pskod 317 28 . . NNP cord-104231-fi8pskod 317 29 , , , cord-104231-fi8pskod 317 30 1990 1990 CD cord-104231-fi8pskod 317 31 ) ) -RRB- cord-104231-fi8pskod 317 32 that that WDT cord-104231-fi8pskod 317 33 can can MD cord-104231-fi8pskod 317 34 be be VB cord-104231-fi8pskod 317 35 used use VBN cord-104231-fi8pskod 317 36 to to TO cord-104231-fi8pskod 317 37 show show VB cord-104231-fi8pskod 317 38 that that IN cord-104231-fi8pskod 317 39 proteins protein NNS cord-104231-fi8pskod 317 40 have have VBP cord-104231-fi8pskod 317 41 reached reach VBN cord-104231-fi8pskod 317 42 this this DT cord-104231-fi8pskod 317 43 part part NN cord-104231-fi8pskod 317 44 of of IN cord-104231-fi8pskod 317 45 the the DT cord-104231-fi8pskod 317 46 Golgi Golgi NNP cord-104231-fi8pskod 317 47 apparatus apparatus NN cord-104231-fi8pskod 317 48 . . . cord-104231-fi8pskod 318 1 Cells cell NNS cord-104231-fi8pskod 318 2 expressing express VBG cord-104231-fi8pskod 318 3 CIM CIM NNP cord-104231-fi8pskod 318 4 or or CC cord-104231-fi8pskod 318 5 CD4 CD4 NNP cord-104231-fi8pskod 318 6 hybrid hybrid NN cord-104231-fi8pskod 318 7 proteins protein NNS cord-104231-fi8pskod 318 8 were be VBD cord-104231-fi8pskod 318 9 labeled label VBN cord-104231-fi8pskod 318 10 with with IN cord-104231-fi8pskod 318 11 [ [ -LRB- cord-104231-fi8pskod 318 12 35S]methionine 35s]methionine CD cord-104231-fi8pskod 318 13 / / SYM cord-104231-fi8pskod 318 14 cysteine cysteine NN cord-104231-fi8pskod 318 15 for for IN cord-104231-fi8pskod 318 16 20 20 CD cord-104231-fi8pskod 318 17 min min NN cord-104231-fi8pskod 318 18 ( ( -LRB- cord-104231-fi8pskod 318 19 see see VB cord-104231-fi8pskod 318 20 Materials material NNS cord-104231-fi8pskod 318 21 and and CC cord-104231-fi8pskod 318 22 Methods Methods NNPS cord-104231-fi8pskod 318 23 ) ) -RRB- cord-104231-fi8pskod 318 24 and and CC cord-104231-fi8pskod 318 25 either either CC cord-104231-fi8pskod 318 26 lysed lyse VBN cord-104231-fi8pskod 318 27 immediately immediately RB cord-104231-fi8pskod 318 28 or or CC cord-104231-fi8pskod 318 29 chased chase VBN cord-104231-fi8pskod 318 30 with with IN cord-104231-fi8pskod 318 31 excess excess JJ cord-104231-fi8pskod 318 32 cold cold JJ cord-104231-fi8pskod 318 33 methionine methionine NN cord-104231-fi8pskod 318 34 and and CC cord-104231-fi8pskod 318 35 cysteine cysteine NN cord-104231-fi8pskod 318 36 for for IN cord-104231-fi8pskod 318 37 2 2 CD cord-104231-fi8pskod 318 38 h. h. NNP cord-104231-fi8pskod 318 39 Immunoprecipitates Immunoprecipitates NNPS cord-104231-fi8pskod 318 40 were be VBD cord-104231-fi8pskod 318 41 treated treat VBN cord-104231-fi8pskod 318 42 with with IN cord-104231-fi8pskod 318 43 neuraminidase neuraminidase NN cord-104231-fi8pskod 318 44 or or CC cord-104231-fi8pskod 318 45 mocktreated mocktreate VBN cord-104231-fi8pskod 318 46 and and CC cord-104231-fi8pskod 318 47 analyzed analyze VBN cord-104231-fi8pskod 318 48 by by IN cord-104231-fi8pskod 318 49 SDS SDS NNP cord-104231-fi8pskod 318 50 - - HYPH cord-104231-fi8pskod 318 51 PAGE PAGE NNP cord-104231-fi8pskod 318 52 ( ( -LRB- cord-104231-fi8pskod 318 53 Fig Fig NNP cord-104231-fi8pskod 318 54 . . . cord-104231-fi8pskod 318 55 8) 8) NNP cord-104231-fi8pskod 318 56 . . . cord-104231-fi8pskod 319 1 Newly newly RB cord-104231-fi8pskod 319 2 synthesized synthesize VBN cord-104231-fi8pskod 319 3 CIM CIM NNP cord-104231-fi8pskod 319 4 was be VBD cord-104231-fi8pskod 319 5 insensitive insensitive JJ cord-104231-fi8pskod 319 6 to to IN cord-104231-fi8pskod 319 7 neuraminidase neuraminidase NN cord-104231-fi8pskod 319 8 ( ( -LRB- cord-104231-fi8pskod 319 9 cf cf NN cord-104231-fi8pskod 319 10 . . . cord-104231-fi8pskod 320 1 lanes lane NNS cord-104231-fi8pskod 320 2 I I NNP cord-104231-fi8pskod 320 3 and and CC cord-104231-fi8pskod 320 4 2 2 CD cord-104231-fi8pskod 320 5 ) ) -RRB- cord-104231-fi8pskod 320 6 but but CC cord-104231-fi8pskod 320 7 completely completely RB cord-104231-fi8pskod 320 8 sensitive sensitive JJ cord-104231-fi8pskod 320 9 after after IN cord-104231-fi8pskod 320 10 a a DT cord-104231-fi8pskod 320 11 2-h 2-h CD cord-104231-fi8pskod 320 12 chase chase NN cord-104231-fi8pskod 320 13 ( ( -LRB- cord-104231-fi8pskod 320 14 cf cf NN cord-104231-fi8pskod 320 15 . . . cord-104231-fi8pskod 321 1 lanes lane NNS cord-104231-fi8pskod 321 2 3 3 CD cord-104231-fi8pskod 321 3 and and CC cord-104231-fi8pskod 321 4 4 4 CD cord-104231-fi8pskod 321 5 ) ) -RRB- cord-104231-fi8pskod 321 6 . . . cord-104231-fi8pskod 322 1 Hybrid Hybrid NNP cord-104231-fi8pskod 322 2 CIM CIM NNP cord-104231-fi8pskod 322 3 proteins protein NNS cord-104231-fi8pskod 322 4 containing contain VBG cord-104231-fi8pskod 322 5 either either CC cord-104231-fi8pskod 322 6 the the DT cord-104231-fi8pskod 322 7 membranespanning membranespanne VBG cord-104231-fi8pskod 322 8 ( ( -LRB- cord-104231-fi8pskod 322 9 CIM CIM NNP cord-104231-fi8pskod 322 10 - - HYPH cord-104231-fi8pskod 322 11 M M NNP cord-104231-fi8pskod 322 12 ) ) -RRB- cord-104231-fi8pskod 322 13 or or CC cord-104231-fi8pskod 322 14 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 322 15 signal signal NN cord-104231-fi8pskod 322 16 ( ( -LRB- cord-104231-fi8pskod 322 17 CIM CIM NNP cord-104231-fi8pskod 322 18 - - HYPH cord-104231-fi8pskod 322 19 C C NNP cord-104231-fi8pskod 322 20 ) ) -RRB- cord-104231-fi8pskod 322 21 were be VBD cord-104231-fi8pskod 322 22 also also RB cord-104231-fi8pskod 322 23 completely completely RB cord-104231-fi8pskod 322 24 siaiylated siaiylate VBN cord-104231-fi8pskod 322 25 after after IN cord-104231-fi8pskod 322 26 a a DT cord-104231-fi8pskod 322 27 2-h 2-h CD cord-104231-fi8pskod 322 28 chase chase NN cord-104231-fi8pskod 322 29 period period NN cord-104231-fi8pskod 322 30 ( ( -LRB- cord-104231-fi8pskod 322 31 lanes lane NNS cord-104231-fi8pskod 322 32 9 9 CD cord-104231-fi8pskod 322 33 - - SYM cord-104231-fi8pskod 322 34 16 16 CD cord-104231-fi8pskod 322 35 ) ) -RRB- cord-104231-fi8pskod 322 36 . . . cord-104231-fi8pskod 323 1 3 3 CD cord-104231-fi8pskod 323 2 , , , cord-104231-fi8pskod 323 3 5 5 CD cord-104231-fi8pskod 323 4 , , , cord-104231-fi8pskod 323 5 7 7 CD cord-104231-fi8pskod 323 6 , , , cord-104231-fi8pskod 323 7 9 9 CD cord-104231-fi8pskod 323 8 , , , cord-104231-fi8pskod 323 9 11 11 CD cord-104231-fi8pskod 323 10 , , , cord-104231-fi8pskod 323 11 13 13 CD cord-104231-fi8pskod 323 12 , , , cord-104231-fi8pskod 323 13 and and CC cord-104231-fi8pskod 323 14 15 15 CD cord-104231-fi8pskod 323 15 ) ) -RRB- cord-104231-fi8pskod 323 16 or or CC cord-104231-fi8pskod 323 17 digested digest VBN cord-104231-fi8pskod 323 18 with with IN cord-104231-fi8pskod 323 19 neuraminidase neuraminidase FW cord-104231-fi8pskod 323 20 ( ( -LRB- cord-104231-fi8pskod 323 21 lanes lane NNS cord-104231-fi8pskod 323 22 2 2 CD cord-104231-fi8pskod 323 23 , , , cord-104231-fi8pskod 323 24 4 4 CD cord-104231-fi8pskod 323 25 , , , cord-104231-fi8pskod 323 26 6 6 CD cord-104231-fi8pskod 323 27 , , , cord-104231-fi8pskod 323 28 8 8 CD cord-104231-fi8pskod 323 29 , , , cord-104231-fi8pskod 323 30 10 10 CD cord-104231-fi8pskod 323 31 , , , cord-104231-fi8pskod 323 32 12 12 CD cord-104231-fi8pskod 323 33 , , , cord-104231-fi8pskod 323 34 14 14 CD cord-104231-fi8pskod 323 35 , , , cord-104231-fi8pskod 323 36 and and CC cord-104231-fi8pskod 323 37 16 16 CD cord-104231-fi8pskod 323 38 ) ) -RRB- cord-104231-fi8pskod 323 39 and and CC cord-104231-fi8pskod 323 40 analyzed analyze VBN cord-104231-fi8pskod 323 41 by by IN cord-104231-fi8pskod 323 42 SDS SDS NNP cord-104231-fi8pskod 323 43 - - HYPH cord-104231-fi8pskod 323 44 PAGE PAGE NNP cord-104231-fi8pskod 323 45 and and CC cord-104231-fi8pskod 323 46 fluorography fluorography NN cord-104231-fi8pskod 323 47 . . . cord-104231-fi8pskod 324 1 The the DT cord-104231-fi8pskod 324 2 molecular molecular JJ cord-104231-fi8pskod 324 3 size size NN cord-104231-fi8pskod 324 4 of of IN cord-104231-fi8pskod 324 5 14C 14c JJ cord-104231-fi8pskod 324 6 - - HYPH cord-104231-fi8pskod 324 7 radioactive radioactive JJ cord-104231-fi8pskod 324 8 markers marker NNS cord-104231-fi8pskod 324 9 are be VBP cord-104231-fi8pskod 324 10 indicated indicate VBN cord-104231-fi8pskod 324 11 on on IN cord-104231-fi8pskod 324 12 the the DT cord-104231-fi8pskod 324 13 left left JJ cord-104231-fi8pskod 324 14 hand hand NN cord-104231-fi8pskod 324 15 side side NN cord-104231-fi8pskod 324 16 of of IN cord-104231-fi8pskod 324 17 the the DT cord-104231-fi8pskod 324 18 panels panel NNS cord-104231-fi8pskod 324 19 . . . cord-104231-fi8pskod 325 1 However however RB cord-104231-fi8pskod 325 2 , , , cord-104231-fi8pskod 325 3 CD4-MC cd4-mc NN cord-104231-fi8pskod 325 4 , , , cord-104231-fi8pskod 325 5 which which WDT cord-104231-fi8pskod 325 6 contains contain VBZ cord-104231-fi8pskod 325 7 both both DT cord-104231-fi8pskod 325 8 signals signal NNS cord-104231-fi8pskod 325 9 , , , cord-104231-fi8pskod 325 10 was be VBD cord-104231-fi8pskod 325 11 incompletely incompletely RB cord-104231-fi8pskod 325 12 sialylated sialylate VBN cord-104231-fi8pskod 325 13 after after IN cord-104231-fi8pskod 325 14 the the DT cord-104231-fi8pskod 325 15 2-h 2-h CD cord-104231-fi8pskod 325 16 chase chase NN cord-104231-fi8pskod 325 17 ( ( -LRB- cord-104231-fi8pskod 325 18 lanes lane NNS cord-104231-fi8pskod 325 19 7and 7and CD cord-104231-fi8pskod 325 20 8) 8) NN cord-104231-fi8pskod 325 21 suggesting suggest VBG cord-104231-fi8pskod 325 22 that that DT cord-104231-fi8pskod 325 23 transport transport NN cord-104231-fi8pskod 325 24 to to IN cord-104231-fi8pskod 325 25 the the DT cord-104231-fi8pskod 325 26 trans trans NNP cord-104231-fi8pskod 325 27 part part NN cord-104231-fi8pskod 325 28 of of IN cord-104231-fi8pskod 325 29 the the DT cord-104231-fi8pskod 325 30 Golgi Golgi NNP cord-104231-fi8pskod 325 31 apparatus apparatus NN cord-104231-fi8pskod 325 32 was be VBD cord-104231-fi8pskod 325 33 slower slow JJR cord-104231-fi8pskod 325 34 . . . cord-104231-fi8pskod 326 1 The the DT cord-104231-fi8pskod 326 2 reason reason NN cord-104231-fi8pskod 326 3 for for IN cord-104231-fi8pskod 326 4 this this DT cord-104231-fi8pskod 326 5 is be VBZ cord-104231-fi8pskod 326 6 unclear unclear JJ cord-104231-fi8pskod 326 7 but but CC cord-104231-fi8pskod 326 8 was be VBD cord-104231-fi8pskod 326 9 not not RB cord-104231-fi8pskod 326 10 the the DT cord-104231-fi8pskod 326 11 consequence consequence NN cord-104231-fi8pskod 326 12 of of IN cord-104231-fi8pskod 326 13 using use VBG cord-104231-fi8pskod 326 14 CD4 CD4 NNP cord-104231-fi8pskod 326 15 as as IN cord-104231-fi8pskod 326 16 the the DT cord-104231-fi8pskod 326 17 reporter reporter NN cord-104231-fi8pskod 326 18 molecule molecule NN cord-104231-fi8pskod 326 19 . . . cord-104231-fi8pskod 327 1 CD8-MC CD8-MC NNP cord-104231-fi8pskod 327 2 was be VBD cord-104231-fi8pskod 327 3 also also RB cord-104231-fi8pskod 327 4 incompletely incompletely RB cord-104231-fi8pskod 327 5 sialylated sialylated JJ cord-104231-fi8pskod 327 6 ( ( -LRB- cord-104231-fi8pskod 327 7 data datum NNS cord-104231-fi8pskod 327 8 not not RB cord-104231-fi8pskod 327 9 shown show VBN cord-104231-fi8pskod 327 10 ) ) -RRB- cord-104231-fi8pskod 327 11 in in IN cord-104231-fi8pskod 327 12 contrast contrast NN cord-104231-fi8pskod 327 13 to to IN cord-104231-fi8pskod 327 14 hybrid hybrid JJ cord-104231-fi8pskod 327 15 CD8 CD8 NNP cord-104231-fi8pskod 327 16 proteins protein NNS cord-104231-fi8pskod 327 17 containing contain VBG cord-104231-fi8pskod 327 18 either either CC cord-104231-fi8pskod 327 19 TGN38 tgn38 CD cord-104231-fi8pskod 327 20 signal signal NN cord-104231-fi8pskod 327 21 ( ( -LRB- cord-104231-fi8pskod 327 22 Fig Fig NNP cord-104231-fi8pskod 327 23 . . . cord-104231-fi8pskod 327 24 9 9 CD cord-104231-fi8pskod 327 25 ) ) -RRB- cord-104231-fi8pskod 327 26 . . . cord-104231-fi8pskod 328 1 Metabolic metabolic JJ cord-104231-fi8pskod 328 2 labeling labeling NN cord-104231-fi8pskod 328 3 of of IN cord-104231-fi8pskod 328 4 CD8 CD8 NNP cord-104231-fi8pskod 328 5 hybrid hybrid NN cord-104231-fi8pskod 328 6 proteins protein NNS cord-104231-fi8pskod 328 7 could could MD cord-104231-fi8pskod 328 8 not not RB cord-104231-fi8pskod 328 9 be be VB cord-104231-fi8pskod 328 10 used use VBN cord-104231-fi8pskod 328 11 to to TO cord-104231-fi8pskod 328 12 study study VB cord-104231-fi8pskod 328 13 arrival arrival NN cord-104231-fi8pskod 328 14 in in IN cord-104231-fi8pskod 328 15 the the DT cord-104231-fi8pskod 328 16 trans trans NNP cord-104231-fi8pskod 328 17 cisterna cisterna NNP cord-104231-fi8pskod 328 18 and and CC cord-104231-fi8pskod 328 19 the the DT cord-104231-fi8pskod 328 20 TGN TGN NNP cord-104231-fi8pskod 328 21 because because IN cord-104231-fi8pskod 328 22 the the DT cord-104231-fi8pskod 328 23 bound bind VBN cord-104231-fi8pskod 328 24 oligosaccharides oligosaccharide NNS cord-104231-fi8pskod 328 25 are be VBP cord-104231-fi8pskod 328 26 all all DT cord-104231-fi8pskod 328 27 O o NN cord-104231-fi8pskod 328 28 - - HYPH cord-104231-fi8pskod 328 29 linked link VBN cord-104231-fi8pskod 328 30 . . . cord-104231-fi8pskod 329 1 Sialylation sialylation NN cord-104231-fi8pskod 329 2 of of IN cord-104231-fi8pskod 329 3 O o NN cord-104231-fi8pskod 329 4 - - HYPH cord-104231-fi8pskod 329 5 linked link VBN cord-104231-fi8pskod 329 6 oligosaccharides oligosaccharide NNS cord-104231-fi8pskod 329 7 is be VBZ cord-104231-fi8pskod 329 8 thought think VBN cord-104231-fi8pskod 329 9 to to TO cord-104231-fi8pskod 329 10 occur occur VB cord-104231-fi8pskod 329 11 in in IN cord-104231-fi8pskod 329 12 the the DT cord-104231-fi8pskod 329 13 medial medial JJ cord-104231-fi8pskod 329 14 - - HYPH cord-104231-fi8pskod 329 15 Golgi Golgi NNP cord-104231-fi8pskod 329 16 cisterna cisterna NN cord-104231-fi8pskod 329 17 ( ( -LRB- cord-104231-fi8pskod 329 18 Locker Locker NNP cord-104231-fi8pskod 329 19 et et FW cord-104231-fi8pskod 329 20 al al NNP cord-104231-fi8pskod 329 21 . . NNP cord-104231-fi8pskod 329 22 , , , cord-104231-fi8pskod 329 23 1992 1992 CD cord-104231-fi8pskod 329 24 ) ) -RRB- cord-104231-fi8pskod 329 25 . . . cord-104231-fi8pskod 330 1 However however RB cord-104231-fi8pskod 330 2 , , , cord-104231-fi8pskod 330 3 pulse pulse NN cord-104231-fi8pskod 330 4 - - HYPH cord-104231-fi8pskod 330 5 chase chase NN cord-104231-fi8pskod 330 6 analysis analysis NN cord-104231-fi8pskod 330 7 combined combine VBN cord-104231-fi8pskod 330 8 with with IN cord-104231-fi8pskod 330 9 neuraminidase neuraminidase JJ cord-104231-fi8pskod 330 10 digestion digestion NN cord-104231-fi8pskod 330 11 showed show VBD cord-104231-fi8pskod 330 12 that that IN cord-104231-fi8pskod 330 13 CD8-M CD8-M NNP cord-104231-fi8pskod 330 14 and and CC cord-104231-fi8pskod 330 15 CD8-C CD8-C NNP cord-104231-fi8pskod 330 16 hybrid hybrid NN cord-104231-fi8pskod 330 17 proteins protein NNS cord-104231-fi8pskod 330 18 had have VBD cord-104231-fi8pskod 330 19 kinetic kinetic JJ cord-104231-fi8pskod 330 20 properties property NNS cord-104231-fi8pskod 330 21 similar similar JJ cord-104231-fi8pskod 330 22 to to IN cord-104231-fi8pskod 330 23 those those DT cord-104231-fi8pskod 330 24 for for IN cord-104231-fi8pskod 330 25 native native JJ cord-104231-fi8pskod 330 26 CD8 CD8 NNP cord-104231-fi8pskod 330 27 ( ( -LRB- cord-104231-fi8pskod 330 28 Fig Fig NNP cord-104231-fi8pskod 330 29 . . . cord-104231-fi8pskod 330 30 9 9 CD cord-104231-fi8pskod 330 31 ) ) -RRB- cord-104231-fi8pskod 330 32 . . . cord-104231-fi8pskod 331 1 In in IN cord-104231-fi8pskod 331 2 all all DT cord-104231-fi8pskod 331 3 cases case NNS cord-104231-fi8pskod 331 4 the the DT cord-104231-fi8pskod 331 5 half half JJ cord-104231-fi8pskod 331 6 - - HYPH cord-104231-fi8pskod 331 7 time time NN cord-104231-fi8pskod 331 8 for for IN cord-104231-fi8pskod 331 9 acquisition acquisition NN cord-104231-fi8pskod 331 10 of of IN cord-104231-fi8pskod 331 11 terminal terminal JJ cord-104231-fi8pskod 331 12 sialic sialic NN cord-104231-fi8pskod 331 13 acid acid NN cord-104231-fi8pskod 331 14 residues residue NNS cord-104231-fi8pskod 331 15 was be VBD cord-104231-fi8pskod 331 16 between between IN cord-104231-fi8pskod 331 17 25 25 CD cord-104231-fi8pskod 331 18 - - SYM cord-104231-fi8pskod 331 19 30 30 CD cord-104231-fi8pskod 331 20 min min NN cord-104231-fi8pskod 331 21 . . . cord-104231-fi8pskod 332 1 Taken take VBN cord-104231-fi8pskod 332 2 further further RB cord-104231-fi8pskod 332 3 with with IN cord-104231-fi8pskod 332 4 the the DT cord-104231-fi8pskod 332 5 results result NNS cord-104231-fi8pskod 332 6 for for IN cord-104231-fi8pskod 332 7 the the DT cord-104231-fi8pskod 332 8 CD4 CD4 NNP cord-104231-fi8pskod 332 9 hybrid hybrid NN cord-104231-fi8pskod 332 10 proteins protein NNS cord-104231-fi8pskod 332 11 , , , cord-104231-fi8pskod 332 12 these these DT cord-104231-fi8pskod 332 13 results result NNS cord-104231-fi8pskod 332 14 show show VBP cord-104231-fi8pskod 332 15 that that IN cord-104231-fi8pskod 332 16 the the DT cord-104231-fi8pskod 332 17 presence presence NN cord-104231-fi8pskod 332 18 of of IN cord-104231-fi8pskod 332 19 either either DT cord-104231-fi8pskod 332 20 signal signal NN cord-104231-fi8pskod 332 21 had have VBD cord-104231-fi8pskod 332 22 little little JJ cord-104231-fi8pskod 332 23 , , , cord-104231-fi8pskod 332 24 if if IN cord-104231-fi8pskod 332 25 any any DT cord-104231-fi8pskod 332 26 , , , cord-104231-fi8pskod 332 27 effect effect NN cord-104231-fi8pskod 332 28 on on IN cord-104231-fi8pskod 332 29 synthesis synthesis NN cord-104231-fi8pskod 332 30 , , , cord-104231-fi8pskod 332 31 transport transport NN cord-104231-fi8pskod 332 32 , , , cord-104231-fi8pskod 332 33 and and CC cord-104231-fi8pskod 332 34 processing processing NN cord-104231-fi8pskod 332 35 of of IN cord-104231-fi8pskod 332 36 the the DT cord-104231-fi8pskod 332 37 reporter reporter NN cord-104231-fi8pskod 332 38 molecules molecule NNS cord-104231-fi8pskod 332 39 . . . cord-104231-fi8pskod 333 1 TGN38 TGN38 NNP cord-104231-fi8pskod 333 2 was be VBD cord-104231-fi8pskod 333 3 originally originally RB cord-104231-fi8pskod 333 4 identified identify VBN cord-104231-fi8pskod 333 5 as as IN cord-104231-fi8pskod 333 6 a a DT cord-104231-fi8pskod 333 7 resident resident NN cord-104231-fi8pskod 333 8 of of IN cord-104231-fi8pskod 333 9 the the DT cord-104231-fi8pskod 333 10 TGN TGN NNP cord-104231-fi8pskod 333 11 ( ( -LRB- cord-104231-fi8pskod 333 12 Luzio Luzio NNP cord-104231-fi8pskod 333 13 et et FW cord-104231-fi8pskod 333 14 al al NNP cord-104231-fi8pskod 333 15 . . NNP cord-104231-fi8pskod 333 16 , , , cord-104231-fi8pskod 333 17 1990 1990 CD cord-104231-fi8pskod 333 18 ) ) -RRB- cord-104231-fi8pskod 333 19 but but CC cord-104231-fi8pskod 333 20 more more JJR cord-104231-fi8pskod 333 21 recent recent JJ cord-104231-fi8pskod 333 22 work work NN cord-104231-fi8pskod 333 23 has have VBZ cord-104231-fi8pskod 333 24 shown show VBN cord-104231-fi8pskod 333 25 that that IN cord-104231-fi8pskod 333 26 it -PRON- PRP cord-104231-fi8pskod 333 27 cycles cycle VBZ cord-104231-fi8pskod 333 28 between between IN cord-104231-fi8pskod 333 29 the the DT cord-104231-fi8pskod 333 30 TGN TGN NNP cord-104231-fi8pskod 333 31 and and CC cord-104231-fi8pskod 333 32 the the DT cord-104231-fi8pskod 333 33 cell cell NN cord-104231-fi8pskod 333 34 surface surface NN cord-104231-fi8pskod 333 35 ( ( -LRB- cord-104231-fi8pskod 333 36 Jones Jones NNP cord-104231-fi8pskod 333 37 et et NNP cord-104231-fi8pskod 333 38 al al NNP cord-104231-fi8pskod 333 39 . . NNP cord-104231-fi8pskod 333 40 , , , cord-104231-fi8pskod 333 41 1993 1993 CD cord-104231-fi8pskod 333 42 ; ; : cord-104231-fi8pskod 333 43 Reaves Reaves NNP cord-104231-fi8pskod 333 44 et et FW cord-104231-fi8pskod 333 45 al al NNP cord-104231-fi8pskod 333 46 . . NNP cord-104231-fi8pskod 333 47 , , , cord-104231-fi8pskod 333 48 1993 1993 CD cord-104231-fi8pskod 333 49 ) ) -RRB- cord-104231-fi8pskod 333 50 . . . cord-104231-fi8pskod 334 1 Movement movement NN cord-104231-fi8pskod 334 2 to to IN cord-104231-fi8pskod 334 3 the the DT cord-104231-fi8pskod 334 4 cell cell NN cord-104231-fi8pskod 334 5 surface surface NN cord-104231-fi8pskod 334 6 occurs occur VBZ cord-104231-fi8pskod 334 7 via via IN cord-104231-fi8pskod 334 8 exocytic exocytic JJ cord-104231-fi8pskod 334 9 vesicles vesicle NNS cord-104231-fi8pskod 335 1 ( ( -LRB- cord-104231-fi8pskod 335 2 Jones Jones NNP cord-104231-fi8pskod 335 3 et et NNP cord-104231-fi8pskod 335 4 al al NNP cord-104231-fi8pskod 335 5 . . NNP cord-104231-fi8pskod 335 6 , , , cord-104231-fi8pskod 335 7 1993 1993 CD cord-104231-fi8pskod 335 8 ) ) -RRB- cord-104231-fi8pskod 335 9 and and CC cord-104231-fi8pskod 335 10 is be VBZ cord-104231-fi8pskod 335 11 followed follow VBN cord-104231-fi8pskod 335 12 by by IN cord-104231-fi8pskod 335 13 retrieval retrieval NN cord-104231-fi8pskod 335 14 which which WDT cord-104231-fi8pskod 335 15 requires require VBZ cord-104231-fi8pskod 335 16 a a DT cord-104231-fi8pskod 335 17 signal signal NN cord-104231-fi8pskod 335 18 present present NN cord-104231-fi8pskod 335 19 in in IN cord-104231-fi8pskod 335 20 the the DT cord-104231-fi8pskod 335 21 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 335 22 domain domain NN cord-104231-fi8pskod 335 23 that that WDT cord-104231-fi8pskod 335 24 has have VBZ cord-104231-fi8pskod 335 25 been be VBN cord-104231-fi8pskod 335 26 localized localize VBN cord-104231-fi8pskod 335 27 to to IN cord-104231-fi8pskod 335 28 the the DT cord-104231-fi8pskod 335 29 sequence sequence NN cord-104231-fi8pskod 335 30 SDYQRL SDYQRL NNP cord-104231-fi8pskod 335 31 ( ( -LRB- cord-104231-fi8pskod 335 32 Bos Bos NNP cord-104231-fi8pskod 335 33 et et NNP cord-104231-fi8pskod 335 34 al al NNP cord-104231-fi8pskod 335 35 . . NNP cord-104231-fi8pskod 335 36 , , , cord-104231-fi8pskod 335 37 1993 1993 CD cord-104231-fi8pskod 335 38 ; ; : cord-104231-fi8pskod 335 39 Humphrey Humphrey NNP cord-104231-fi8pskod 335 40 et et NNP cord-104231-fi8pskod 335 41 ai ai NNP cord-104231-fi8pskod 335 42 . . . cord-104231-fi8pskod 336 1 , , , cord-104231-fi8pskod 336 2 1993 1993 CD cord-104231-fi8pskod 336 3 ; ; : cord-104231-fi8pskod 336 4 Wong Wong NNP cord-104231-fi8pskod 336 5 and and CC cord-104231-fi8pskod 336 6 Hong Hong NNP cord-104231-fi8pskod 336 7 , , , cord-104231-fi8pskod 336 8 1993 1993 CD cord-104231-fi8pskod 336 9 ) ) -RRB- cord-104231-fi8pskod 336 10 . . . cord-104231-fi8pskod 337 1 Retrieved retrieve VBN cord-104231-fi8pskod 337 2 TGN38 tgn38 ADD cord-104231-fi8pskod 338 1 first first JJ cord-104231-fi8pskod 338 2 moves move NNS cord-104231-fi8pskod 338 3 to to IN cord-104231-fi8pskod 338 4 the the DT cord-104231-fi8pskod 338 5 endosomes endosome NNS cord-104231-fi8pskod 338 6 and and CC cord-104231-fi8pskod 338 7 then then RB cord-104231-fi8pskod 338 8 to to IN cord-104231-fi8pskod 338 9 the the DT cord-104231-fi8pskod 338 10 TGN TGN NNP cord-104231-fi8pskod 338 11 ( ( -LRB- cord-104231-fi8pskod 338 12 Bos Bos NNP cord-104231-fi8pskod 338 13 et et NNP cord-104231-fi8pskod 338 14 al al NNP cord-104231-fi8pskod 338 15 . . NNP cord-104231-fi8pskod 338 16 , , , cord-104231-fi8pskod 338 17 1993 1993 CD cord-104231-fi8pskod 338 18 ; ; : cord-104231-fi8pskod 339 1 Humphrey Humphrey NNP cord-104231-fi8pskod 339 2 et et NNP cord-104231-fi8pskod 339 3 al al NNP cord-104231-fi8pskod 339 4 . . . cord-104231-fi8pskod 340 1 , , , cord-104231-fi8pskod 340 2 1993 1993 CD cord-104231-fi8pskod 340 3 ) ) -RRB- cord-104231-fi8pskod 340 4 . . . cord-104231-fi8pskod 341 1 Using use VBG cord-104231-fi8pskod 341 2 plasma plasma NN cord-104231-fi8pskod 341 3 membrane membrane NN cord-104231-fi8pskod 341 4 glycoproteins glycoprotein NNS cord-104231-fi8pskod 341 5 as as IN cord-104231-fi8pskod 341 6 reporter reporter NN cord-104231-fi8pskod 341 7 molecules molecule NNS cord-104231-fi8pskod 341 8 , , , cord-104231-fi8pskod 341 9 we -PRON- PRP cord-104231-fi8pskod 341 10 have have VBP cord-104231-fi8pskod 341 11 now now RB cord-104231-fi8pskod 341 12 shown show VBN cord-104231-fi8pskod 341 13 that that IN cord-104231-fi8pskod 341 14 there there EX cord-104231-fi8pskod 341 15 is be VBZ cord-104231-fi8pskod 341 16 an an DT cord-104231-fi8pskod 341 17 additional additional JJ cord-104231-fi8pskod 341 18 signal signal NN cord-104231-fi8pskod 341 19 for for IN cord-104231-fi8pskod 341 20 localizing localize VBG cord-104231-fi8pskod 341 21 the the DT cord-104231-fi8pskod 341 22 protein protein NN cord-104231-fi8pskod 341 23 to to IN cord-104231-fi8pskod 341 24 the the DT cord-104231-fi8pskod 341 25 TGN TGN NNP cord-104231-fi8pskod 341 26 . . . cord-104231-fi8pskod 342 1 This this DT cord-104231-fi8pskod 342 2 signal signal NN cord-104231-fi8pskod 342 3 is be VBZ cord-104231-fi8pskod 342 4 located locate VBN cord-104231-fi8pskod 342 5 in in IN cord-104231-fi8pskod 342 6 the the DT cord-104231-fi8pskod 342 7 membrane membrane NN cord-104231-fi8pskod 342 8 - - HYPH cord-104231-fi8pskod 342 9 spanning span VBG cord-104231-fi8pskod 342 10 domain domain NN cord-104231-fi8pskod 342 11 . . . cord-104231-fi8pskod 343 1 Four four CD cord-104231-fi8pskod 343 2 independent independent JJ cord-104231-fi8pskod 343 3 lines line NNS cord-104231-fi8pskod 343 4 of of IN cord-104231-fi8pskod 343 5 evidence evidence NN cord-104231-fi8pskod 343 6 support support VBP cord-104231-fi8pskod 343 7 this this DT cord-104231-fi8pskod 343 8 conclusion conclusion NN cord-104231-fi8pskod 343 9 . . . cord-104231-fi8pskod 344 1 First first RB cord-104231-fi8pskod 344 2 , , , cord-104231-fi8pskod 344 3 immunofluorescence immunofluorescence NN cord-104231-fi8pskod 344 4 microscopy microscopy NNS cord-104231-fi8pskod 344 5 showed show VBD cord-104231-fi8pskod 344 6 that that IN cord-104231-fi8pskod 344 7 CD4-M CD4-M NNP cord-104231-fi8pskod 344 8 and and CC cord-104231-fi8pskod 344 9 CD8-M CD8-M NNP cord-104231-fi8pskod 344 10 hybrid hybrid JJ cord-104231-fi8pskod 344 11 proteins protein NNS cord-104231-fi8pskod 344 12 localized localize VBN cord-104231-fi8pskod 344 13 to to IN cord-104231-fi8pskod 344 14 a a DT cord-104231-fi8pskod 344 15 juxtanuclear juxtanuclear NN cord-104231-fi8pskod 344 16 reticulum reticulum NN cord-104231-fi8pskod 344 17 that that WDT cord-104231-fi8pskod 344 18 could could MD cord-104231-fi8pskod 344 19 also also RB cord-104231-fi8pskod 344 20 be be VB cord-104231-fi8pskod 344 21 stained stain VBN cord-104231-fi8pskod 344 22 by by IN cord-104231-fi8pskod 344 23 antibodies antibody NNS cord-104231-fi8pskod 344 24 to to IN cord-104231-fi8pskod 344 25 GAIT GAIT NNP cord-104231-fi8pskod 344 26 , , , cord-104231-fi8pskod 344 27 a a DT cord-104231-fi8pskod 344 28 marker marker NN cord-104231-fi8pskod 344 29 for for IN cord-104231-fi8pskod 344 30 the the DT cord-104231-fi8pskod 344 31 trans trans NNP cord-104231-fi8pskod 344 32 cisterna cisterna NNP cord-104231-fi8pskod 344 33 and and CC cord-104231-fi8pskod 344 34 TGN TGN NNP cord-104231-fi8pskod 344 35 ( ( -LRB- cord-104231-fi8pskod 344 36 Roth Roth NNP cord-104231-fi8pskod 344 37 and and CC cord-104231-fi8pskod 344 38 Berger Berger NNP cord-104231-fi8pskod 344 39 , , , cord-104231-fi8pskod 344 40 1982 1982 CD cord-104231-fi8pskod 344 41 ; ; : cord-104231-fi8pskod 345 1 Taatjes Taatjes NNP cord-104231-fi8pskod 345 2 et et NNP cord-104231-fi8pskod 345 3 al al NNP cord-104231-fi8pskod 345 4 . . . cord-104231-fi8pskod 346 1 , , , cord-104231-fi8pskod 346 2 1992 1992 CD cord-104231-fi8pskod 346 3 ; ; : cord-104231-fi8pskod 346 4 Nilsson Nilsson NNP cord-104231-fi8pskod 346 5 et et NNP cord-104231-fi8pskod 346 6 al al NNP cord-104231-fi8pskod 346 7 . . NNP cord-104231-fi8pskod 346 8 , , , cord-104231-fi8pskod 346 9 1993a 1993a CD cord-104231-fi8pskod 346 10 ) ) -RRB- cord-104231-fi8pskod 346 11 . . . cord-104231-fi8pskod 347 1 This this DT cord-104231-fi8pskod 347 2 staining staining NN cord-104231-fi8pskod 347 3 pattern pattern NN cord-104231-fi8pskod 347 4 was be VBD cord-104231-fi8pskod 347 5 not not RB cord-104231-fi8pskod 347 6 affected affect VBN cord-104231-fi8pskod 347 7 by by IN cord-104231-fi8pskod 347 8 pretreamaent pretreamaent NN cord-104231-fi8pskod 347 9 with with IN cord-104231-fi8pskod 347 10 10 10 CD cord-104231-fi8pskod 347 11 # # $ cord-104231-fi8pskod 347 12 g g NN cord-104231-fi8pskod 347 13 / / SYM cord-104231-fi8pskod 347 14 ml ml NN cord-104231-fi8pskod 347 15 cycloheximide cycloheximide NN cord-104231-fi8pskod 347 16 for for IN cord-104231-fi8pskod 347 17 up up IN cord-104231-fi8pskod 347 18 to to TO cord-104231-fi8pskod 347 19 7 7 CD cord-104231-fi8pskod 347 20 h h NN cord-104231-fi8pskod 347 21 ( ( -LRB- cord-104231-fi8pskod 347 22 data datum NNS cord-104231-fi8pskod 347 23 not not RB cord-104231-fi8pskod 347 24 shown show VBN cord-104231-fi8pskod 347 25 ) ) -RRB- cord-104231-fi8pskod 347 26 showing show VBG cord-104231-fi8pskod 347 27 that that IN cord-104231-fi8pskod 347 28 these these DT cord-104231-fi8pskod 347 29 hybrid hybrid JJ cord-104231-fi8pskod 347 30 proteins protein NNS cord-104231-fi8pskod 347 31 were be VBD cord-104231-fi8pskod 347 32 not not RB cord-104231-fi8pskod 347 33 simply simply RB cord-104231-fi8pskod 347 34 moving move VBG cord-104231-fi8pskod 347 35 slowly slowly RB cord-104231-fi8pskod 347 36 along along IN cord-104231-fi8pskod 347 37 the the DT cord-104231-fi8pskod 347 38 exocytic exocytic JJ cord-104231-fi8pskod 347 39 pathway pathway NN cord-104231-fi8pskod 347 40 . . . cord-104231-fi8pskod 348 1 In in IN cord-104231-fi8pskod 348 2 addition addition NN cord-104231-fi8pskod 348 3 , , , cord-104231-fi8pskod 348 4 it -PRON- PRP cord-104231-fi8pskod 348 5 is be VBZ cord-104231-fi8pskod 348 6 unlikely unlikely JJ cord-104231-fi8pskod 348 7 that that IN cord-104231-fi8pskod 348 8 a a DT cord-104231-fi8pskod 348 9 cryptic cryptic JJ cord-104231-fi8pskod 348 10 localization localization NN cord-104231-fi8pskod 348 11 signal signal NN cord-104231-fi8pskod 348 12 in in IN cord-104231-fi8pskod 348 13 the the DT cord-104231-fi8pskod 348 14 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 348 15 domain domain NN cord-104231-fi8pskod 348 16 of of IN cord-104231-fi8pskod 348 17 either either CC cord-104231-fi8pskod 348 18 CD4 CD4 NNP cord-104231-fi8pskod 348 19 or or CC cord-104231-fi8pskod 348 20 CD8 CD8 NNP cord-104231-fi8pskod 348 21 was be VBD cord-104231-fi8pskod 348 22 activated activate VBN cord-104231-fi8pskod 348 23 as as IN cord-104231-fi8pskod 348 24 a a DT cord-104231-fi8pskod 348 25 result result NN cord-104231-fi8pskod 348 26 of of IN cord-104231-fi8pskod 348 27 generating generate VBG cord-104231-fi8pskod 348 28 hybrid hybrid NN cord-104231-fi8pskod 348 29 proteins protein NNS cord-104231-fi8pskod 348 30 . . . cord-104231-fi8pskod 349 1 Both both DT cord-104231-fi8pskod 349 2 CD8-MAC CD8-MAC NNP cord-104231-fi8pskod 349 3 and and CC cord-104231-fi8pskod 349 4 CD4-MAC CD4-MAC NNP cord-104231-fi8pskod 349 5 hybrid hybrid NN cord-104231-fi8pskod 349 6 proteins protein NNS cord-104231-fi8pskod 349 7 which which WDT cord-104231-fi8pskod 349 8 lack lack VBP cord-104231-fi8pskod 349 9 most most JJS cord-104231-fi8pskod 349 10 of of IN cord-104231-fi8pskod 349 11 the the DT cord-104231-fi8pskod 349 12 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 349 13 domain domain NN cord-104231-fi8pskod 349 14 ( ( -LRB- cord-104231-fi8pskod 349 15 including include VBG cord-104231-fi8pskod 349 16 the the DT cord-104231-fi8pskod 349 17 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 349 18 signal signal NN cord-104231-fi8pskod 349 19 for for IN cord-104231-fi8pskod 349 20 TGN38 TGN38 NNS cord-104231-fi8pskod 349 21 ) ) -RRB- cord-104231-fi8pskod 349 22 were be VBD cord-104231-fi8pskod 349 23 also also RB cord-104231-fi8pskod 349 24 localized localize VBN cord-104231-fi8pskod 349 25 to to IN cord-104231-fi8pskod 349 26 the the DT cord-104231-fi8pskod 349 27 Golgi Golgi NNP cord-104231-fi8pskod 349 28 apparatus apparatus NN cord-104231-fi8pskod 349 29 . . . cord-104231-fi8pskod 350 1 Second second RB cord-104231-fi8pskod 350 2 , , , cord-104231-fi8pskod 350 3 quantitative quantitative JJ cord-104231-fi8pskod 350 4 immunoelectron immunoelectron NNP cord-104231-fi8pskod 350 5 microscopy microscopy NNS cord-104231-fi8pskod 350 6 showed show VBD cord-104231-fi8pskod 350 7 that that IN cord-104231-fi8pskod 350 8 CIM CIM NNP cord-104231-fi8pskod 350 9 - - HYPH cord-104231-fi8pskod 350 10 M M NNP cord-104231-fi8pskod 350 11 had have VBD cord-104231-fi8pskod 350 12 almost almost RB cord-104231-fi8pskod 350 13 the the DT cord-104231-fi8pskod 350 14 same same JJ cord-104231-fi8pskod 350 15 distribution distribution NN cord-104231-fi8pskod 350 16 as as IN cord-104231-fi8pskod 350 17 TGN38 TGN38 NNS cord-104231-fi8pskod 350 18 at at IN cord-104231-fi8pskod 350 19 both both CC cord-104231-fi8pskod 350 20 high high JJ cord-104231-fi8pskod 350 21 and and CC cord-104231-fi8pskod 350 22 low low JJ cord-104231-fi8pskod 350 23 levels level NNS cord-104231-fi8pskod 350 24 of of IN cord-104231-fi8pskod 350 25 expression expression NN cord-104231-fi8pskod 350 26 . . . cord-104231-fi8pskod 351 1 At at IN cord-104231-fi8pskod 351 2 low low JJ cord-104231-fi8pskod 351 3 levels level NNS cord-104231-fi8pskod 351 4 of of IN cord-104231-fi8pskod 351 5 expression expression NN cord-104231-fi8pskod 351 6 , , , cord-104231-fi8pskod 351 7 all all DT cord-104231-fi8pskod 351 8 of of IN cord-104231-fi8pskod 351 9 the the DT cord-104231-fi8pskod 351 10 CD4-M CD4-M NNP cord-104231-fi8pskod 351 11 hybrid hybrid NN cord-104231-fi8pskod 351 12 protein protein NN cord-104231-fi8pskod 351 13 and and CC cord-104231-fi8pskod 351 14 TGN38 TGN38 NNS cord-104231-fi8pskod 351 15 was be VBD cord-104231-fi8pskod 351 16 present present JJ cord-104231-fi8pskod 351 17 in in IN cord-104231-fi8pskod 351 18 the the DT cord-104231-fi8pskod 351 19 Golgi Golgi NNP cord-104231-fi8pskod 351 20 apparatus apparatus NN cord-104231-fi8pskod 351 21 , , , cord-104231-fi8pskod 351 22 80 80 CD cord-104231-fi8pskod 351 23 % % NN cord-104231-fi8pskod 351 24 being be VBG cord-104231-fi8pskod 351 25 present present JJ cord-104231-fi8pskod 351 26 within within IN cord-104231-fi8pskod 351 27 the the DT cord-104231-fi8pskod 351 28 TGN TGN NNP cord-104231-fi8pskod 351 29 . . . cord-104231-fi8pskod 352 1 At at IN cord-104231-fi8pskod 352 2 higher high JJR cord-104231-fi8pskod 352 3 levels level NNS cord-104231-fi8pskod 352 4 of of IN cord-104231-fi8pskod 352 5 expression expression NN cord-104231-fi8pskod 352 6 , , , cord-104231-fi8pskod 352 7 CIM CIM NNP cord-104231-fi8pskod 352 8 - - HYPH cord-104231-fi8pskod 352 9 M M NNP cord-104231-fi8pskod 352 10 and and CC cord-104231-fi8pskod 352 11 TGN38 TGN38 NNS cord-104231-fi8pskod 352 12 were be VBD cord-104231-fi8pskod 352 13 also also RB cord-104231-fi8pskod 352 14 present present JJ cord-104231-fi8pskod 352 15 in in IN cord-104231-fi8pskod 352 16 endosomes endosome NNS cord-104231-fi8pskod 352 17 and and CC cord-104231-fi8pskod 352 18 on on IN cord-104231-fi8pskod 352 19 the the DT cord-104231-fi8pskod 352 20 cell cell NN cord-104231-fi8pskod 352 21 surface surface NN cord-104231-fi8pskod 352 22 , , , cord-104231-fi8pskod 352 23 again again RB cord-104231-fi8pskod 352 24 to to IN cord-104231-fi8pskod 352 25 a a DT cord-104231-fi8pskod 352 26 similar similar JJ cord-104231-fi8pskod 352 27 extent extent NN cord-104231-fi8pskod 352 28 . . . cord-104231-fi8pskod 353 1 Even even RB cord-104231-fi8pskod 353 2 at at IN cord-104231-fi8pskod 353 3 these these DT cord-104231-fi8pskod 353 4 higher high JJR cord-104231-fi8pskod 353 5 levels level NNS cord-104231-fi8pskod 353 6 of of IN cord-104231-fi8pskod 353 7 expression expression NN cord-104231-fi8pskod 353 8 , , , cord-104231-fi8pskod 353 9 both both DT cord-104231-fi8pskod 353 10 proteins protein NNS cord-104231-fi8pskod 353 11 were be VBD cord-104231-fi8pskod 353 12 still still RB cord-104231-fi8pskod 353 13 concentrated concentrate VBN cord-104231-fi8pskod 353 14 in in IN cord-104231-fi8pskod 353 15 the the DT cord-104231-fi8pskod 353 16 TGN TGN NNP cord-104231-fi8pskod 353 17 as as IN cord-104231-fi8pskod 353 18 compared compare VBN cord-104231-fi8pskod 353 19 to to IN cord-104231-fi8pskod 353 20 the the DT cord-104231-fi8pskod 353 21 plasma plasma NN cord-104231-fi8pskod 353 22 membrane membrane NN cord-104231-fi8pskod 353 23 . . . cord-104231-fi8pskod 354 1 For for IN cord-104231-fi8pskod 354 2 TGN38 TGN38 NNS cord-104231-fi8pskod 354 3 , , , cord-104231-fi8pskod 354 4 the the DT cord-104231-fi8pskod 354 5 linear linear JJ cord-104231-fi8pskod 354 6 density density NN cord-104231-fi8pskod 354 7 in in IN cord-104231-fi8pskod 354 8 the the DT cord-104231-fi8pskod 354 9 TGN TGN NNP cord-104231-fi8pskod 354 10 was be VBD cord-104231-fi8pskod 354 11 four four CD cord-104231-fi8pskod 354 12 times time NNS cord-104231-fi8pskod 354 13 that that IN cord-104231-fi8pskod 354 14 in in IN cord-104231-fi8pskod 354 15 the the DT cord-104231-fi8pskod 354 16 plasma plasma NN cord-104231-fi8pskod 354 17 membrane membrane NN cord-104231-fi8pskod 354 18 ; ; : cord-104231-fi8pskod 354 19 for for IN cord-104231-fi8pskod 354 20 CD4-M CD4-M NNP cord-104231-fi8pskod 354 21 it -PRON- PRP cord-104231-fi8pskod 354 22 was be VBD cord-104231-fi8pskod 354 23 even even RB cord-104231-fi8pskod 354 24 higher high JJR cord-104231-fi8pskod 354 25 , , , cord-104231-fi8pskod 354 26 being be VBG cord-104231-fi8pskod 354 27 12 12 CD cord-104231-fi8pskod 354 28 times time NNS cord-104231-fi8pskod 354 29 higher high JJR cord-104231-fi8pskod 354 30 in in IN cord-104231-fi8pskod 354 31 the the DT cord-104231-fi8pskod 354 32 TGN TGN NNP cord-104231-fi8pskod 354 33 than than IN cord-104231-fi8pskod 354 34 in in IN cord-104231-fi8pskod 354 35 the the DT cord-104231-fi8pskod 354 36 plasma plasma NN cord-104231-fi8pskod 354 37 membrane membrane NN cord-104231-fi8pskod 354 38 . . . cord-104231-fi8pskod 355 1 The the DT cord-104231-fi8pskod 355 2 distribution distribution NN cord-104231-fi8pskod 355 3 of of IN cord-104231-fi8pskod 355 4 CD8-M CD8-M NNP cord-104231-fi8pskod 355 5 was be VBD cord-104231-fi8pskod 355 6 also also RB cord-104231-fi8pskod 355 7 similar similar JJ cord-104231-fi8pskod 355 8 to to IN cord-104231-fi8pskod 355 9 that that DT cord-104231-fi8pskod 355 10 for for IN cord-104231-fi8pskod 355 11 TGN38 TGN38 NNS cord-104231-fi8pskod 355 12 being be VBG cord-104231-fi8pskod 355 13 exclusively exclusively RB cord-104231-fi8pskod 355 14 in in IN cord-104231-fi8pskod 355 15 the the DT cord-104231-fi8pskod 355 16 Golgi Golgi NNP cord-104231-fi8pskod 355 17 apparatus apparatus NN cord-104231-fi8pskod 355 18 at at IN cord-104231-fi8pskod 355 19 low low JJ cord-104231-fi8pskod 355 20 levels level NNS cord-104231-fi8pskod 355 21 of of IN cord-104231-fi8pskod 355 22 expression expression NN cord-104231-fi8pskod 355 23 . . . cord-104231-fi8pskod 356 1 There there EX cord-104231-fi8pskod 356 2 was be VBD cord-104231-fi8pskod 356 3 , , , cord-104231-fi8pskod 356 4 however however RB cord-104231-fi8pskod 356 5 , , , cord-104231-fi8pskod 356 6 more more JJR cord-104231-fi8pskod 356 7 of of IN cord-104231-fi8pskod 356 8 this this DT cord-104231-fi8pskod 356 9 hybrid hybrid JJ cord-104231-fi8pskod 356 10 protein protein NN cord-104231-fi8pskod 356 11 in in IN cord-104231-fi8pskod 356 12 the the DT cord-104231-fi8pskod 356 13 Golgi Golgi NNP cord-104231-fi8pskod 356 14 stack stack NN cord-104231-fi8pskod 356 15 for for IN cord-104231-fi8pskod 356 16 reasons reason NNS cord-104231-fi8pskod 356 17 that that WDT cord-104231-fi8pskod 356 18 are be VBP cord-104231-fi8pskod 356 19 still still RB cord-104231-fi8pskod 356 20 unclear unclear JJ cord-104231-fi8pskod 356 21 . . . cord-104231-fi8pskod 357 1 Third third JJ cord-104231-fi8pskod 357 2 , , , cord-104231-fi8pskod 357 3 FACS FACS NNP cord-104231-fi8pskod 357 4 analysis analysis NN cord-104231-fi8pskod 357 5 showed show VBD cord-104231-fi8pskod 357 6 that that IN cord-104231-fi8pskod 357 7 the the DT cord-104231-fi8pskod 357 8 membrane membrane NN cord-104231-fi8pskod 357 9 - - HYPH cord-104231-fi8pskod 357 10 spanning span VBG cord-104231-fi8pskod 357 11 domain domain NN cord-104231-fi8pskod 357 12 reduced reduce VBD cord-104231-fi8pskod 357 13 the the DT cord-104231-fi8pskod 357 14 percentage percentage NN cord-104231-fi8pskod 357 15 of of IN cord-104231-fi8pskod 357 16 positive positive JJ cord-104231-fi8pskod 357 17 cells cell NNS cord-104231-fi8pskod 357 18 from from IN cord-104231-fi8pskod 357 19 58 58 CD cord-104231-fi8pskod 357 20 % % NN cord-104231-fi8pskod 357 21 Figure figure NN cord-104231-fi8pskod 357 22 9 9 CD cord-104231-fi8pskod 357 23 . . . cord-104231-fi8pskod 358 1 Metabolic metabolic JJ cord-104231-fi8pskod 358 2 labeling labeling NN cord-104231-fi8pskod 358 3 of of IN cord-104231-fi8pskod 358 4 CD8 CD8 NNP cord-104231-fi8pskod 358 5 and and CC cord-104231-fi8pskod 358 6 CD8 CD8 NNP cord-104231-fi8pskod 358 7 hybrid hybrid NN cord-104231-fi8pskod 358 8 proteins protein NNS cord-104231-fi8pskod 358 9 . . . cord-104231-fi8pskod 359 1 Transfected Transfected NNP cord-104231-fi8pskod 359 2 HeLa HeLa NNP cord-104231-fi8pskod 359 3 ceils ceil NNS cord-104231-fi8pskod 359 4 were be VBD cord-104231-fi8pskod 359 5 labeled label VBN cord-104231-fi8pskod 359 6 with with IN cord-104231-fi8pskod 359 7 [ [ -LRB- cord-104231-fi8pskod 359 8 35S]methionine 35s]methionine CD cord-104231-fi8pskod 359 9 / / SYM cord-104231-fi8pskod 359 10 cysteine cysteine NN cord-104231-fi8pskod 359 11 for for IN cord-104231-fi8pskod 359 12 20 20 CD cord-104231-fi8pskod 359 13 min min NN cord-104231-fi8pskod 359 14 and and CC cord-104231-fi8pskod 359 15 chased chase VBD cord-104231-fi8pskod 359 16 for0 for0 NNS cord-104231-fi8pskod 359 17 , , , cord-104231-fi8pskod 359 18 15 15 CD cord-104231-fi8pskod 359 19 , , , cord-104231-fi8pskod 359 20 30 30 CD cord-104231-fi8pskod 359 21 , , , cord-104231-fi8pskod 359 22 45 45 CD cord-104231-fi8pskod 359 23 , , , cord-104231-fi8pskod 359 24 75 75 CD cord-104231-fi8pskod 359 25 , , , cord-104231-fi8pskod 359 26 or or CC cord-104231-fi8pskod 359 27 120 120 CD cord-104231-fi8pskod 359 28 min min NN cord-104231-fi8pskod 359 29 . . . cord-104231-fi8pskod 360 1 Immunoprecipitates Immunoprecipitates NNP cord-104231-fi8pskod 360 2 were be VBD cord-104231-fi8pskod 360 3 mock mock JJ cord-104231-fi8pskod 360 4 - - HYPH cord-104231-fi8pskod 360 5 digested digested JJ cord-104231-fi8pskod 360 6 ( ( -LRB- cord-104231-fi8pskod 360 7 - - : cord-104231-fi8pskod 360 8 ) ) -RRB- cord-104231-fi8pskod 360 9 or or CC cord-104231-fi8pskod 360 10 digested digest VBN cord-104231-fi8pskod 360 11 ( ( -LRB- cord-104231-fi8pskod 360 12 + + CC cord-104231-fi8pskod 360 13 ) ) -RRB- cord-104231-fi8pskod 360 14 with with IN cord-104231-fi8pskod 360 15 neurarninidase neurarninidase NNP cord-104231-fi8pskod 360 16 and and CC cord-104231-fi8pskod 360 17 analyzed analyze VBN cord-104231-fi8pskod 360 18 by by IN cord-104231-fi8pskod 360 19 SDS SDS NNP cord-104231-fi8pskod 360 20 - - HYPH cord-104231-fi8pskod 360 21 PAGE PAGE NNP cord-104231-fi8pskod 360 22 and and CC cord-104231-fi8pskod 360 23 fluorography fluorography NN cord-104231-fi8pskod 360 24 . . . cord-104231-fi8pskod 361 1 ( ( -LRB- cord-104231-fi8pskod 361 2 for for IN cord-104231-fi8pskod 361 3 CD8 CD8 NNP cord-104231-fi8pskod 361 4 ) ) -RRB- cord-104231-fi8pskod 361 5 to to IN cord-104231-fi8pskod 361 6 13 13 CD cord-104231-fi8pskod 361 7 % % NN cord-104231-fi8pskod 361 8 ( ( -LRB- cord-104231-fi8pskod 361 9 for for IN cord-104231-fi8pskod 361 10 CD8-M CD8-M NNP cord-104231-fi8pskod 361 11 ) ) -RRB- cord-104231-fi8pskod 361 12 , , , cord-104231-fi8pskod 361 13 a a DT cord-104231-fi8pskod 361 14 4.5-fold 4.5-fold CD cord-104231-fi8pskod 361 15 decrease decrease NN cord-104231-fi8pskod 361 16 . . . cord-104231-fi8pskod 362 1 This this DT cord-104231-fi8pskod 362 2 effect effect NN cord-104231-fi8pskod 362 3 was be VBD cord-104231-fi8pskod 362 4 not not RB cord-104231-fi8pskod 362 5 the the DT cord-104231-fi8pskod 362 6 consequence consequence NN cord-104231-fi8pskod 362 7 of of IN cord-104231-fi8pskod 362 8 activating activate VBG cord-104231-fi8pskod 362 9 a a DT cord-104231-fi8pskod 362 10 cryptic cryptic JJ cord-104231-fi8pskod 362 11 signal signal NN cord-104231-fi8pskod 362 12 in in IN cord-104231-fi8pskod 362 13 the the DT cord-104231-fi8pskod 362 14 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 362 15 domain domain NN cord-104231-fi8pskod 362 16 of of IN cord-104231-fi8pskod 362 17 CD8 CD8 NNP cord-104231-fi8pskod 362 18 since since IN cord-104231-fi8pskod 362 19 removal removal NN cord-104231-fi8pskod 362 20 of of IN cord-104231-fi8pskod 362 21 most most JJS cord-104231-fi8pskod 362 22 of of IN cord-104231-fi8pskod 362 23 this this DT cord-104231-fi8pskod 362 24 domain domain NN cord-104231-fi8pskod 362 25 ( ( -LRB- cord-104231-fi8pskod 362 26 including include VBG cord-104231-fi8pskod 362 27 the the DT cord-104231-fi8pskod 362 28 TGN38 tgn38 CD cord-104231-fi8pskod 362 29 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 362 30 signal signal NN cord-104231-fi8pskod 362 31 ) ) -RRB- cord-104231-fi8pskod 362 32 still still RB cord-104231-fi8pskod 362 33 reduced reduce VBD cord-104231-fi8pskod 362 34 the the DT cord-104231-fi8pskod 362 35 percentage percentage NN cord-104231-fi8pskod 362 36 of of IN cord-104231-fi8pskod 362 37 positive positive JJ cord-104231-fi8pskod 362 38 ceils ceil NNS cord-104231-fi8pskod 362 39 to to IN cord-104231-fi8pskod 362 40 19 19 CD cord-104231-fi8pskod 362 41 % % NN cord-104231-fi8pskod 362 42 ( ( -LRB- cord-104231-fi8pskod 362 43 for for IN cord-104231-fi8pskod 362 44 CD8-MAC CD8-MAC NNP cord-104231-fi8pskod 362 45 ) ) -RRB- cord-104231-fi8pskod 362 46 , , , cord-104231-fi8pskod 362 47 a a DT cord-104231-fi8pskod 362 48 threefold threefold JJ cord-104231-fi8pskod 362 49 decrease decrease NN cord-104231-fi8pskod 362 50 . . . cord-104231-fi8pskod 363 1 Fourth fourth JJ cord-104231-fi8pskod 363 2 and and CC cord-104231-fi8pskod 363 3 finally finally RB cord-104231-fi8pskod 363 4 , , , cord-104231-fi8pskod 363 5 metabolic metabolic JJ cord-104231-fi8pskod 363 6 labeling labeling NN cord-104231-fi8pskod 363 7 studies study NNS cord-104231-fi8pskod 363 8 showed show VBD cord-104231-fi8pskod 363 9 that that IN cord-104231-fi8pskod 363 10 all all DT cord-104231-fi8pskod 363 11 molecules molecule NNS cord-104231-fi8pskod 363 12 of of IN cord-104231-fi8pskod 363 13 CD4-M CD4-M NNP cord-104231-fi8pskod 363 14 received receive VBD cord-104231-fi8pskod 363 15 sialic sialic JJ cord-104231-fi8pskod 363 16 acid acid NN cord-104231-fi8pskod 363 17 , , , cord-104231-fi8pskod 363 18 showing show VBG cord-104231-fi8pskod 363 19 that that IN cord-104231-fi8pskod 363 20 they -PRON- PRP cord-104231-fi8pskod 363 21 had have VBD cord-104231-fi8pskod 363 22 all all RB cord-104231-fi8pskod 363 23 reached reach VBN cord-104231-fi8pskod 363 24 the the DT cord-104231-fi8pskod 363 25 trans trans NNP cord-104231-fi8pskod 363 26 cisterna cisterna NNP cord-104231-fi8pskod 363 27 / / SYM cord-104231-fi8pskod 363 28 TGN TGN NNP cord-104231-fi8pskod 363 29 . . . cord-104231-fi8pskod 364 1 The the DT cord-104231-fi8pskod 364 2 present present JJ cord-104231-fi8pskod 364 3 studies study NNS cord-104231-fi8pskod 364 4 are be VBP cord-104231-fi8pskod 364 5 at at IN cord-104231-fi8pskod 364 6 variance variance NN cord-104231-fi8pskod 364 7 with with IN cord-104231-fi8pskod 364 8 previous previous JJ cord-104231-fi8pskod 364 9 studies study NNS cord-104231-fi8pskod 364 10 that that WDT cord-104231-fi8pskod 364 11 identified identify VBD cord-104231-fi8pskod 364 12 only only RB cord-104231-fi8pskod 364 13 the the DT cord-104231-fi8pskod 364 14 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 364 15 domain domain NN cord-104231-fi8pskod 364 16 of of IN cord-104231-fi8pskod 364 17 TGN38 TGN38 NNS cord-104231-fi8pskod 364 18 as as IN cord-104231-fi8pskod 364 19 a a DT cord-104231-fi8pskod 364 20 localiTation localitation NN cord-104231-fi8pskod 364 21 signal signal NN cord-104231-fi8pskod 364 22 . . . cord-104231-fi8pskod 365 1 Removal removal NN cord-104231-fi8pskod 365 2 of of IN cord-104231-fi8pskod 365 3 most most JJS cord-104231-fi8pskod 365 4 of of IN cord-104231-fi8pskod 365 5 the the DT cord-104231-fi8pskod 365 6 domain domain NN cord-104231-fi8pskod 365 7 led lead VBD cord-104231-fi8pskod 365 8 to to IN cord-104231-fi8pskod 365 9 the the DT cord-104231-fi8pskod 365 10 appearance appearance NN cord-104231-fi8pskod 365 11 of of IN cord-104231-fi8pskod 365 12 the the DT cord-104231-fi8pskod 365 13 truncated truncated JJ cord-104231-fi8pskod 365 14 protein protein NN cord-104231-fi8pskod 365 15 on on IN cord-104231-fi8pskod 365 16 the the DT cord-104231-fi8pskod 365 17 cell cell NN cord-104231-fi8pskod 365 18 surface surface NN cord-104231-fi8pskod 366 1 ( ( -LRB- cord-104231-fi8pskod 366 2 Luzio Luzio NNP cord-104231-fi8pskod 366 3 et et FW cord-104231-fi8pskod 366 4 al al NNP cord-104231-fi8pskod 366 5 . . . cord-104231-fi8pskod 367 1 , , , cord-104231-fi8pskod 367 2 1990 1990 CD cord-104231-fi8pskod 367 3 ; ; : cord-104231-fi8pskod 367 4 Bos Bos NNP cord-104231-fi8pskod 367 5 et et NNP cord-104231-fi8pskod 367 6 al al NNP cord-104231-fi8pskod 367 7 . . NNP cord-104231-fi8pskod 367 8 , , , cord-104231-fi8pskod 367 9 1993 1993 CD cord-104231-fi8pskod 367 10 ) ) -RRB- cord-104231-fi8pskod 367 11 . . . cord-104231-fi8pskod 368 1 It -PRON- PRP cord-104231-fi8pskod 368 2 should should MD cord-104231-fi8pskod 368 3 be be VB cord-104231-fi8pskod 368 4 noted note VBN cord-104231-fi8pskod 368 5 that that IN cord-104231-fi8pskod 368 6 the the DT cord-104231-fi8pskod 368 7 earher earher NNP cord-104231-fi8pskod 368 8 study study NN cord-104231-fi8pskod 368 9 was be VBD cord-104231-fi8pskod 368 10 carried carry VBN cord-104231-fi8pskod 368 11 out out RP cord-104231-fi8pskod 368 12 before before IN cord-104231-fi8pskod 368 13 any any DT cord-104231-fi8pskod 368 14 evidence evidence NN cord-104231-fi8pskod 368 15 existed exist VBD cord-104231-fi8pskod 368 16 for for IN cord-104231-fi8pskod 368 17 retention retention NN cord-104231-fi8pskod 368 18 by by IN cord-104231-fi8pskod 368 19 membrane membrane NN cord-104231-fi8pskod 368 20 - - HYPH cord-104231-fi8pskod 368 21 spanning span VBG cord-104231-fi8pskod 368 22 domains domain NNS cord-104231-fi8pskod 368 23 , , , cord-104231-fi8pskod 368 24 a a DT cord-104231-fi8pskod 368 25 phenomenon phenomenon NN cord-104231-fi8pskod 368 26 first first RB cord-104231-fi8pskod 368 27 described describe VBN cord-104231-fi8pskod 368 28 for for IN cord-104231-fi8pskod 368 29 a a DT cord-104231-fi8pskod 368 30 viral viral JJ cord-104231-fi8pskod 368 31 protein protein NN cord-104231-fi8pskod 368 32 ( ( -LRB- cord-104231-fi8pskod 368 33 Swift Swift NNP cord-104231-fi8pskod 368 34 and and CC cord-104231-fi8pskod 368 35 Machamer Machamer NNP cord-104231-fi8pskod 368 36 , , , cord-104231-fi8pskod 368 37 1991 1991 CD cord-104231-fi8pskod 368 38 ) ) -RRB- cord-104231-fi8pskod 368 39 and and CC cord-104231-fi8pskod 368 40 Golgi Golgi NNP cord-104231-fi8pskod 368 41 enzymes enzyme NNS cord-104231-fi8pskod 368 42 ( ( -LRB- cord-104231-fi8pskod 368 43 Nilsson Nilsson NNP cord-104231-fi8pskod 368 44 et et NNP cord-104231-fi8pskod 368 45 al al NNP cord-104231-fi8pskod 368 46 . . NNP cord-104231-fi8pskod 368 47 , , , cord-104231-fi8pskod 368 48 1991 1991 CD cord-104231-fi8pskod 368 49 ; ; : cord-104231-fi8pskod 368 50 Munro Munro NNP cord-104231-fi8pskod 368 51 , , , cord-104231-fi8pskod 368 52 1991 1991 CD cord-104231-fi8pskod 368 53 ) ) -RRB- cord-104231-fi8pskod 368 54 . . . cord-104231-fi8pskod 369 1 The the DT cord-104231-fi8pskod 369 2 expectation expectation NN cord-104231-fi8pskod 369 3 was be VBD cord-104231-fi8pskod 369 4 that that IN cord-104231-fi8pskod 369 5 the the DT cord-104231-fi8pskod 369 6 signal signal NN cord-104231-fi8pskod 369 7 would would MD cord-104231-fi8pskod 369 8 be be VB cord-104231-fi8pskod 369 9 found find VBN cord-104231-fi8pskod 369 10 in in IN cord-104231-fi8pskod 369 11 the the DT cord-104231-fi8pskod 369 12 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 369 13 domain domain NN cord-104231-fi8pskod 369 14 and and CC cord-104231-fi8pskod 369 15 this this DT cord-104231-fi8pskod 369 16 appeared appear VBD cord-104231-fi8pskod 369 17 to to TO cord-104231-fi8pskod 369 18 be be VB cord-104231-fi8pskod 369 19 borne bear VBN cord-104231-fi8pskod 369 20 out out RP cord-104231-fi8pskod 369 21 by by IN cord-104231-fi8pskod 369 22 the the DT cord-104231-fi8pskod 369 23 appearance appearance NN cord-104231-fi8pskod 369 24 of of IN cord-104231-fi8pskod 369 25 protein protein NN cord-104231-fi8pskod 369 26 on on IN cord-104231-fi8pskod 369 27 the the DT cord-104231-fi8pskod 369 28 cell cell NN cord-104231-fi8pskod 369 29 surface surface NN cord-104231-fi8pskod 369 30 . . . cord-104231-fi8pskod 370 1 The the DT cord-104231-fi8pskod 370 2 likely likely JJ cord-104231-fi8pskod 370 3 reason reason NN cord-104231-fi8pskod 370 4 for for IN cord-104231-fi8pskod 370 5 this this DT cord-104231-fi8pskod 370 6 , , , cord-104231-fi8pskod 370 7 however however RB cord-104231-fi8pskod 370 8 , , , cord-104231-fi8pskod 370 9 was be VBD cord-104231-fi8pskod 370 10 that that IN cord-104231-fi8pskod 370 11 the the DT cord-104231-fi8pskod 370 12 levels level NNS cord-104231-fi8pskod 370 13 of of IN cord-104231-fi8pskod 370 14 expressed express VBN cord-104231-fi8pskod 370 15 protein protein NN cord-104231-fi8pskod 370 16 were be VBD cord-104231-fi8pskod 370 17 so so RB cord-104231-fi8pskod 370 18 high high JJ cord-104231-fi8pskod 370 19 that that IN cord-104231-fi8pskod 370 20 they -PRON- PRP cord-104231-fi8pskod 370 21 saturated saturate VBD cord-104231-fi8pskod 370 22 the the DT cord-104231-fi8pskod 370 23 retention retention NN cord-104231-fi8pskod 370 24 mechanism mechanism NN cord-104231-fi8pskod 370 25 that that WDT cord-104231-fi8pskod 370 26 operates operate VBZ cord-104231-fi8pskod 370 27 on on IN cord-104231-fi8pskod 370 28 the the DT cord-104231-fi8pskod 370 29 membrane membrane NN cord-104231-fi8pskod 370 30 - - HYPH cord-104231-fi8pskod 370 31 spanning span VBG cord-104231-fi8pskod 370 32 domain domain NN cord-104231-fi8pskod 370 33 . . . cord-104231-fi8pskod 371 1 Even even RB cord-104231-fi8pskod 371 2 cells cell NNS cord-104231-fi8pskod 371 3 expressing express VBG cord-104231-fi8pskod 371 4 the the DT cord-104231-fi8pskod 371 5 wild wild JJ cord-104231-fi8pskod 371 6 - - HYPH cord-104231-fi8pskod 371 7 type type NN cord-104231-fi8pskod 371 8 protein protein NN cord-104231-fi8pskod 371 9 ( ( -LRB- cord-104231-fi8pskod 371 10 TGN38 TGN38 NNP cord-104231-fi8pskod 371 11 ) ) -RRB- cord-104231-fi8pskod 371 12 , , , cord-104231-fi8pskod 371 13 containing contain VBG cord-104231-fi8pskod 371 14 both both CC cord-104231-fi8pskod 371 15 retention retention NN cord-104231-fi8pskod 371 16 and and CC cord-104231-fi8pskod 371 17 retrieval retrieval NN cord-104231-fi8pskod 371 18 signals signal NNS cord-104231-fi8pskod 371 19 , , , cord-104231-fi8pskod 371 20 showed show VBD cord-104231-fi8pskod 371 21 aberrant aberrant JJ cord-104231-fi8pskod 371 22 localization localization NN cord-104231-fi8pskod 371 23 . . . cord-104231-fi8pskod 372 1 After after IN cord-104231-fi8pskod 372 2 24 24 CD cord-104231-fi8pskod 372 3 h h NN cord-104231-fi8pskod 372 4 transfection transfection NN cord-104231-fi8pskod 372 5 , , , cord-104231-fi8pskod 372 6 50 50 CD cord-104231-fi8pskod 372 7 % % NN cord-104231-fi8pskod 372 8 of of IN cord-104231-fi8pskod 372 9 the the DT cord-104231-fi8pskod 372 10 cells cell NNS cord-104231-fi8pskod 372 11 had have VBD cord-104231-fi8pskod 372 12 either either CC cord-104231-fi8pskod 372 13 fallen fall VBN cord-104231-fi8pskod 372 14 off off IN cord-104231-fi8pskod 372 15 the the DT cord-104231-fi8pskod 372 16 coverslip coverslip NN cord-104231-fi8pskod 372 17 or or CC cord-104231-fi8pskod 372 18 showed show VBD cord-104231-fi8pskod 372 19 cell cell NN cord-104231-fi8pskod 372 20 surface surface NN cord-104231-fi8pskod 372 21 staining staining NN cord-104231-fi8pskod 372 22 ( ( -LRB- cord-104231-fi8pskod 372 23 Luzio Luzio NNP cord-104231-fi8pskod 372 24 et et FW cord-104231-fi8pskod 372 25 al al NNP cord-104231-fi8pskod 372 26 . . NNP cord-104231-fi8pskod 372 27 , , , cord-104231-fi8pskod 372 28 1990 1990 CD cord-104231-fi8pskod 372 29 ) ) -RRB- cord-104231-fi8pskod 372 30 . . . cord-104231-fi8pskod 373 1 The the DT cord-104231-fi8pskod 373 2 more more RBR cord-104231-fi8pskod 373 3 recent recent JJ cord-104231-fi8pskod 373 4 study study NN cord-104231-fi8pskod 373 5 by by IN cord-104231-fi8pskod 373 6 Bos Bos NNP cord-104231-fi8pskod 373 7 et et NNP cord-104231-fi8pskod 373 8 al al NNP cord-104231-fi8pskod 373 9 . . . cord-104231-fi8pskod 374 1 ( ( -LRB- cord-104231-fi8pskod 374 2 1993 1993 CD cord-104231-fi8pskod 374 3 ) ) -RRB- cord-104231-fi8pskod 374 4 overcame overcome VBD cord-104231-fi8pskod 374 5 this this DT cord-104231-fi8pskod 374 6 problem problem NN cord-104231-fi8pskod 374 7 by by IN cord-104231-fi8pskod 374 8 using use VBG cord-104231-fi8pskod 374 9 a a DT cord-104231-fi8pskod 374 10 similar similar JJ cord-104231-fi8pskod 374 11 expression expression NN cord-104231-fi8pskod 374 12 vector vector NN cord-104231-fi8pskod 374 13 ( ( -LRB- cord-104231-fi8pskod 374 14 pSVL1 psvl1 NN cord-104231-fi8pskod 374 15 ) ) -RRB- cord-104231-fi8pskod 374 16 at at IN cord-104231-fi8pskod 374 17 a a DT cord-104231-fi8pskod 374 18 low low JJ cord-104231-fi8pskod 374 19 transfection transfection NN cord-104231-fi8pskod 374 20 efficiency efficiency NN cord-104231-fi8pskod 374 21 ( ( -LRB- cord-104231-fi8pskod 374 22 ~0.15 ~0.15 CD cord-104231-fi8pskod 374 23 % % NN cord-104231-fi8pskod 374 24 ) ) -RRB- cord-104231-fi8pskod 374 25 to to TO cord-104231-fi8pskod 374 26 ensure ensure VB cord-104231-fi8pskod 374 27 more more RBR cord-104231-fi8pskod 374 28 constant constant JJ cord-104231-fi8pskod 374 29 levels level NNS cord-104231-fi8pskod 374 30 of of IN cord-104231-fi8pskod 374 31 expression expression NN cord-104231-fi8pskod 374 32 in in IN cord-104231-fi8pskod 374 33 each each DT cord-104231-fi8pskod 374 34 cell cell NN cord-104231-fi8pskod 374 35 . . . cord-104231-fi8pskod 375 1 Unfortunately unfortunately RB cord-104231-fi8pskod 375 2 , , , cord-104231-fi8pskod 375 3 their -PRON- PRP$ cord-104231-fi8pskod 375 4 only only JJ cord-104231-fi8pskod 375 5 assay assay NN cord-104231-fi8pskod 375 6 was be VBD cord-104231-fi8pskod 375 7 immunofluorescence immunofluorescence NN cord-104231-fi8pskod 375 8 microscopy microscopy NN cord-104231-fi8pskod 375 9 and and CC cord-104231-fi8pskod 375 10 it -PRON- PRP cord-104231-fi8pskod 375 11 was be VBD cord-104231-fi8pskod 375 12 impossible impossible JJ cord-104231-fi8pskod 375 13 to to TO cord-104231-fi8pskod 375 14 determine determine VB cord-104231-fi8pskod 375 15 how how WRB cord-104231-fi8pskod 375 16 much much JJ cord-104231-fi8pskod 375 17 of of IN cord-104231-fi8pskod 375 18 the the DT cord-104231-fi8pskod 375 19 protein protein NN cord-104231-fi8pskod 375 20 was be VBD cord-104231-fi8pskod 375 21 still still RB cord-104231-fi8pskod 375 22 present present JJ cord-104231-fi8pskod 375 23 in in IN cord-104231-fi8pskod 375 24 the the DT cord-104231-fi8pskod 375 25 Golgi Golgi NNP cord-104231-fi8pskod 375 26 apparatus apparatus NN cord-104231-fi8pskod 375 27 . . . cord-104231-fi8pskod 376 1 These these DT cord-104231-fi8pskod 376 2 problems problem NNS cord-104231-fi8pskod 376 3 did do VBD cord-104231-fi8pskod 376 4 not not RB cord-104231-fi8pskod 376 5 arise arise VB cord-104231-fi8pskod 376 6 in in IN cord-104231-fi8pskod 376 7 our -PRON- PRP$ cord-104231-fi8pskod 376 8 system system NN cord-104231-fi8pskod 376 9 since since IN cord-104231-fi8pskod 376 10 the the DT cord-104231-fi8pskod 376 11 expressed express VBN cord-104231-fi8pskod 376 12 levels level NNS cord-104231-fi8pskod 376 13 of of IN cord-104231-fi8pskod 376 14 hybrid hybrid JJ cord-104231-fi8pskod 376 15 protein protein NN cord-104231-fi8pskod 376 16 were be VBD cord-104231-fi8pskod 376 17 lower low JJR cord-104231-fi8pskod 376 18 and and CC cord-104231-fi8pskod 376 19 the the DT cord-104231-fi8pskod 376 20 distributions distribution NNS cord-104231-fi8pskod 376 21 were be VBD cord-104231-fi8pskod 376 22 quantitated quantitate VBN cord-104231-fi8pskod 376 23 in in IN cord-104231-fi8pskod 376 24 several several JJ cord-104231-fi8pskod 376 25 independent independent JJ cord-104231-fi8pskod 376 26 ways way NNS cord-104231-fi8pskod 376 27 . . . cord-104231-fi8pskod 377 1 Two two CD cord-104231-fi8pskod 377 2 different different JJ cord-104231-fi8pskod 377 3 reporter reporter NN cord-104231-fi8pskod 377 4 molecules molecule NNS cord-104231-fi8pskod 377 5 were be VBD cord-104231-fi8pskod 377 6 also also RB cord-104231-fi8pskod 377 7 used use VBN cord-104231-fi8pskod 377 8 . . . cord-104231-fi8pskod 378 1 However however RB cord-104231-fi8pskod 378 2 , , , cord-104231-fi8pskod 378 3 it -PRON- PRP cord-104231-fi8pskod 378 4 is be VBZ cord-104231-fi8pskod 378 5 conceivable conceivable JJ cord-104231-fi8pskod 378 6 that that IN cord-104231-fi8pskod 378 7 other other JJ cord-104231-fi8pskod 378 8 small small JJ cord-104231-fi8pskod 378 9 differences difference NNS cord-104231-fi8pskod 378 10 in in IN cord-104231-fi8pskod 378 11 the the DT cord-104231-fi8pskod 378 12 systems system NNS cord-104231-fi8pskod 378 13 used use VBN cord-104231-fi8pskod 378 14 are be VBP cord-104231-fi8pskod 378 15 responsible responsible JJ cord-104231-fi8pskod 378 16 for for IN cord-104231-fi8pskod 378 17 the the DT cord-104231-fi8pskod 378 18 apparent apparent JJ cord-104231-fi8pskod 378 19 discrepancies discrepancy NNS cord-104231-fi8pskod 378 20 . . . cord-104231-fi8pskod 379 1 Wong Wong NNP cord-104231-fi8pskod 379 2 and and CC cord-104231-fi8pskod 379 3 Hong Hong NNP cord-104231-fi8pskod 379 4 ( ( -LRB- cord-104231-fi8pskod 379 5 1993 1993 CD cord-104231-fi8pskod 379 6 ) ) -RRB- cord-104231-fi8pskod 379 7 , , , cord-104231-fi8pskod 379 8 for for IN cord-104231-fi8pskod 379 9 example example NN cord-104231-fi8pskod 379 10 , , , cord-104231-fi8pskod 379 11 found find VBD cord-104231-fi8pskod 379 12 that that IN cord-104231-fi8pskod 379 13 the the DT cord-104231-fi8pskod 379 14 serine serine NN cord-104231-fi8pskod 379 15 residue residue NN cord-104231-fi8pskod 379 16 in in IN cord-104231-fi8pskod 379 17 the the DT cord-104231-fi8pskod 379 18 SDYQRL SDYQRL NNP cord-104231-fi8pskod 379 19 motif motif NN cord-104231-fi8pskod 379 20 was be VBD cord-104231-fi8pskod 379 21 important important JJ cord-104231-fi8pskod 379 22 for for IN cord-104231-fi8pskod 379 23 Golgi Golgi NNP cord-104231-fi8pskod 379 24 localization localization NN cord-104231-fi8pskod 379 25 , , , cord-104231-fi8pskod 379 26 while while IN cord-104231-fi8pskod 379 27 Humphrey Humphrey NNP cord-104231-fi8pskod 379 28 et et NNP cord-104231-fi8pskod 379 29 al al NNP cord-104231-fi8pskod 379 30 . . . cord-104231-fi8pskod 380 1 ( ( -LRB- cord-104231-fi8pskod 380 2 1993 1993 CD cord-104231-fi8pskod 380 3 ) ) -RRB- cord-104231-fi8pskod 380 4 found find VBD cord-104231-fi8pskod 380 5 that that IN cord-104231-fi8pskod 380 6 an an DT cord-104231-fi8pskod 380 7 identical identical JJ cord-104231-fi8pskod 380 8 mutation mutation NN cord-104231-fi8pskod 380 9 in in IN cord-104231-fi8pskod 380 10 their -PRON- PRP$ cord-104231-fi8pskod 380 11 system system NN cord-104231-fi8pskod 380 12 had have VBD cord-104231-fi8pskod 380 13 no no DT cord-104231-fi8pskod 380 14 effect effect NN cord-104231-fi8pskod 380 15 . . . cord-104231-fi8pskod 381 1 Further further JJ cord-104231-fi8pskod 381 2 studies study NNS cord-104231-fi8pskod 381 3 using use VBG cord-104231-fi8pskod 381 4 stable stable JJ cord-104231-fi8pskod 381 5 cell cell NN cord-104231-fi8pskod 381 6 lines line NNS cord-104231-fi8pskod 381 7 will will MD cord-104231-fi8pskod 381 8 be be VB cord-104231-fi8pskod 381 9 needed need VBN cord-104231-fi8pskod 381 10 to to TO cord-104231-fi8pskod 381 11 resolve resolve VB cord-104231-fi8pskod 381 12 these these DT cord-104231-fi8pskod 381 13 differences difference NNS cord-104231-fi8pskod 381 14 . . . cord-104231-fi8pskod 382 1 Though though IN cord-104231-fi8pskod 382 2 the the DT cord-104231-fi8pskod 382 3 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 382 4 and and CC cord-104231-fi8pskod 382 5 membrane membrane NN cord-104231-fi8pskod 382 6 - - HYPH cord-104231-fi8pskod 382 7 spanning span VBG cord-104231-fi8pskod 382 8 signals signal NNS cord-104231-fi8pskod 382 9 can can MD cord-104231-fi8pskod 382 10 act act VB cord-104231-fi8pskod 382 11 independently independently RB cord-104231-fi8pskod 382 12 to to TO cord-104231-fi8pskod 382 13 localize localize VB cord-104231-fi8pskod 382 14 hybrid hybrid JJ cord-104231-fi8pskod 382 15 proteins protein NNS cord-104231-fi8pskod 382 16 to to IN cord-104231-fi8pskod 382 17 the the DT cord-104231-fi8pskod 382 18 TGN TGN NNP cord-104231-fi8pskod 382 19 , , , cord-104231-fi8pskod 382 20 it -PRON- PRP cord-104231-fi8pskod 382 21 is be VBZ cord-104231-fi8pskod 382 22 likely likely JJ cord-104231-fi8pskod 382 23 that that IN cord-104231-fi8pskod 382 24 they -PRON- PRP cord-104231-fi8pskod 382 25 operate operate VBP cord-104231-fi8pskod 382 26 in in IN cord-104231-fi8pskod 382 27 different different JJ cord-104231-fi8pskod 382 28 ways way NNS cord-104231-fi8pskod 382 29 . . . cord-104231-fi8pskod 383 1 The the DT cord-104231-fi8pskod 383 2 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 383 3 domain domain NN cord-104231-fi8pskod 383 4 is be VBZ cord-104231-fi8pskod 383 5 a a DT cord-104231-fi8pskod 383 6 clear clear JJ cord-104231-fi8pskod 383 7 example example NN cord-104231-fi8pskod 383 8 of of IN cord-104231-fi8pskod 383 9 a a DT cord-104231-fi8pskod 383 10 retrieval retrieval NN cord-104231-fi8pskod 383 11 signal signal NN cord-104231-fi8pskod 383 12 . . . cord-104231-fi8pskod 384 1 This this DT cord-104231-fi8pskod 384 2 acts act VBZ cord-104231-fi8pskod 384 3 after after IN cord-104231-fi8pskod 384 4 a a DT cord-104231-fi8pskod 384 5 protein protein NN cord-104231-fi8pskod 384 6 has have VBZ cord-104231-fi8pskod 384 7 left leave VBN cord-104231-fi8pskod 384 8 the the DT cord-104231-fi8pskod 384 9 compartment compartment NN cord-104231-fi8pskod 384 10 in in IN cord-104231-fi8pskod 384 11 which which WDT cord-104231-fi8pskod 384 12 it -PRON- PRP cord-104231-fi8pskod 384 13 norreally norreally RB cord-104231-fi8pskod 384 14 functions function VBZ cord-104231-fi8pskod 384 15 and and CC cord-104231-fi8pskod 384 16 returns return VBZ cord-104231-fi8pskod 384 17 them -PRON- PRP cord-104231-fi8pskod 384 18 from from IN cord-104231-fi8pskod 384 19 a a DT cord-104231-fi8pskod 384 20 later later JJ cord-104231-fi8pskod 384 21 compartment compartment NN cord-104231-fi8pskod 384 22 . . . cord-104231-fi8pskod 385 1 All all DT cord-104231-fi8pskod 385 2 of of IN cord-104231-fi8pskod 385 3 the the DT cord-104231-fi8pskod 385 4 retrieval retrieval NN cord-104231-fi8pskod 385 5 signals signal NNS cord-104231-fi8pskod 385 6 so so RB cord-104231-fi8pskod 385 7 far far RB cord-104231-fi8pskod 385 8 characterized characterize VBN cord-104231-fi8pskod 385 9 have have VBP cord-104231-fi8pskod 385 10 been be VBN cord-104231-fi8pskod 385 11 located locate VBN cord-104231-fi8pskod 385 12 in in IN cord-104231-fi8pskod 385 13 the the DT cord-104231-fi8pskod 385 14 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 385 15 domains domain NNS cord-104231-fi8pskod 385 16 of of IN cord-104231-fi8pskod 385 17 proteins protein NNS cord-104231-fi8pskod 385 18 and and CC cord-104231-fi8pskod 385 19 many many JJ cord-104231-fi8pskod 385 20 are be VBP cord-104231-fi8pskod 385 21 based base VBN cord-104231-fi8pskod 385 22 on on IN cord-104231-fi8pskod 385 23 tyrosine tyrosine NN cord-104231-fi8pskod 385 24 as as IN cord-104231-fi8pskod 385 25 the the DT cord-104231-fi8pskod 385 26 critical critical JJ cord-104231-fi8pskod 385 27 amino amino NN cord-104231-fi8pskod 385 28 acid acid NN cord-104231-fi8pskod 385 29 set set VBN cord-104231-fi8pskod 385 30 in in IN cord-104231-fi8pskod 385 31 a a DT cord-104231-fi8pskod 385 32 particular particular JJ cord-104231-fi8pskod 385 33 motif motif NN cord-104231-fi8pskod 385 34 ( ( -LRB- cord-104231-fi8pskod 385 35 for for IN cord-104231-fi8pskod 385 36 review review NN cord-104231-fi8pskod 385 37 see see VB cord-104231-fi8pskod 385 38 Collawn Collawn NNP cord-104231-fi8pskod 385 39 et et NNP cord-104231-fi8pskod 385 40 al al NNP cord-104231-fi8pskod 385 41 . . NNP cord-104231-fi8pskod 385 42 , , , cord-104231-fi8pskod 385 43 1990 1990 CD cord-104231-fi8pskod 385 44 ; ; : cord-104231-fi8pskod 385 45 Pearse Pearse NNP cord-104231-fi8pskod 385 46 and and CC cord-104231-fi8pskod 385 47 Robinson Robinson NNP cord-104231-fi8pskod 385 48 , , , cord-104231-fi8pskod 385 49 1990 1990 CD cord-104231-fi8pskod 385 50 ; ; : cord-104231-fi8pskod 385 51 Kornfeld Kornfeld NNP cord-104231-fi8pskod 385 52 , , , cord-104231-fi8pskod 385 53 1992 1992 CD cord-104231-fi8pskod 385 54 ; ; : cord-104231-fi8pskod 385 55 Matter Matter NNP cord-104231-fi8pskod 385 56 et et NNP cord-104231-fi8pskod 385 57 ai ai NNP cord-104231-fi8pskod 385 58 . . NNP cord-104231-fi8pskod 385 59 , , , cord-104231-fi8pskod 385 60 1992 1992 CD cord-104231-fi8pskod 385 61 ) ) -RRB- cord-104231-fi8pskod 385 62 . . . cord-104231-fi8pskod 386 1 The the DT cord-104231-fi8pskod 386 2 critical critical JJ cord-104231-fi8pskod 386 3 motif motif NN cord-104231-fi8pskod 386 4 in in IN cord-104231-fi8pskod 386 5 the the DT cord-104231-fi8pskod 386 6 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 386 7 domain domain NN cord-104231-fi8pskod 386 8 of of IN cord-104231-fi8pskod 386 9 TGN38 TGN38 NNS cord-104231-fi8pskod 386 10 is be VBZ cord-104231-fi8pskod 386 11 SDYQRL SDYQRL NNP cord-104231-fi8pskod 386 12 ( ( -LRB- cord-104231-fi8pskod 387 1 Bos Bos NNP cord-104231-fi8pskod 387 2 et et NNP cord-104231-fi8pskod 387 3 al al NNP cord-104231-fi8pskod 387 4 . . . cord-104231-fi8pskod 388 1 , , , cord-104231-fi8pskod 388 2 1993 1993 CD cord-104231-fi8pskod 388 3 ; ; : cord-104231-fi8pskod 388 4 Humphrey Humphrey NNP cord-104231-fi8pskod 388 5 et et NNP cord-104231-fi8pskod 388 6 al al NNP cord-104231-fi8pskod 388 7 . . . cord-104231-fi8pskod 389 1 , , , cord-104231-fi8pskod 389 2 1993 1993 CD cord-104231-fi8pskod 389 3 ; ; : cord-104231-fi8pskod 389 4 Wong Wong NNP cord-104231-fi8pskod 389 5 and and CC cord-104231-fi8pskod 389 6 Hong Hong NNP cord-104231-fi8pskod 389 7 , , , cord-104231-fi8pskod 389 8 1993 1993 CD cord-104231-fi8pskod 389 9 ) ) -RRB- cord-104231-fi8pskod 389 10 and and CC cord-104231-fi8pskod 389 11 it -PRON- PRP cord-104231-fi8pskod 389 12 operates operate VBZ cord-104231-fi8pskod 389 13 once once IN cord-104231-fi8pskod 389 14 the the DT cord-104231-fi8pskod 389 15 protein protein NN cord-104231-fi8pskod 389 16 reaches reach VBZ cord-104231-fi8pskod 389 17 the the DT cord-104231-fi8pskod 389 18 cell cell NN cord-104231-fi8pskod 389 19 surface surface NN cord-104231-fi8pskod 389 20 . . . cord-104231-fi8pskod 390 1 TGN38 TGN38 NNP cord-104231-fi8pskod 390 2 is be VBZ cord-104231-fi8pskod 390 3 then then RB cord-104231-fi8pskod 390 4 returned return VBN cord-104231-fi8pskod 390 5 to to IN cord-104231-fi8pskod 390 6 the the DT cord-104231-fi8pskod 390 7 TGN TGN NNP cord-104231-fi8pskod 390 8 via via IN cord-104231-fi8pskod 390 9 endosomes endosome NNS cord-104231-fi8pskod 390 10 . . . cord-104231-fi8pskod 391 1 The the DT cord-104231-fi8pskod 391 2 membrane membrane NN cord-104231-fi8pskod 391 3 - - HYPH cord-104231-fi8pskod 391 4 spanning span VBG cord-104231-fi8pskod 391 5 domain domain NN cord-104231-fi8pskod 391 6 is be VBZ cord-104231-fi8pskod 391 7 a a DT cord-104231-fi8pskod 391 8 typical typical JJ cord-104231-fi8pskod 391 9 Golgi Golgi NNP cord-104231-fi8pskod 391 10 retention retention NN cord-104231-fi8pskod 391 11 signal signal NN cord-104231-fi8pskod 391 12 . . . cord-104231-fi8pskod 392 1 These these DT cord-104231-fi8pskod 392 2 act act VBP cord-104231-fi8pskod 392 3 to to TO cord-104231-fi8pskod 392 4 keep keep VB cord-104231-fi8pskod 392 5 proteins protein NNS cord-104231-fi8pskod 392 6 in in IN cord-104231-fi8pskod 392 7 the the DT cord-104231-fi8pskod 392 8 Golgi Golgi NNP cord-104231-fi8pskod 392 9 and and CC cord-104231-fi8pskod 392 10 , , , cord-104231-fi8pskod 392 11 so so RB cord-104231-fi8pskod 392 12 far far RB cord-104231-fi8pskod 392 13 , , , cord-104231-fi8pskod 392 14 all all DT cord-104231-fi8pskod 392 15 Golgi Golgi NNP cord-104231-fi8pskod 392 16 proteins protein NNS cord-104231-fi8pskod 392 17 ( ( -LRB- cord-104231-fi8pskod 392 18 Machamer Machamer NNP cord-104231-fi8pskod 392 19 and and CC cord-104231-fi8pskod 392 20 Swift Swift NNP cord-104231-fi8pskod 392 21 , , , cord-104231-fi8pskod 392 22 1991 1991 CD cord-104231-fi8pskod 392 23 ) ) -RRB- cord-104231-fi8pskod 392 24 and and CC cord-104231-fi8pskod 392 25 enzymes enzyme NNS cord-104231-fi8pskod 392 26 ( ( -LRB- cord-104231-fi8pskod 392 27 Nilsson Nilsson NNP cord-104231-fi8pskod 392 28 et et NNP cord-104231-fi8pskod 392 29 al al NNP cord-104231-fi8pskod 392 30 . . NNP cord-104231-fi8pskod 392 31 , , , cord-104231-fi8pskod 392 32 1991 1991 CD cord-104231-fi8pskod 392 33 ; ; : cord-104231-fi8pskod 392 34 Munro Munro NNP cord-104231-fi8pskod 392 35 , , , cord-104231-fi8pskod 392 36 1991 1991 CD cord-104231-fi8pskod 392 37 ; ; : cord-104231-fi8pskod 392 38 Aoki Aoki NNP cord-104231-fi8pskod 392 39 et et NNP cord-104231-fi8pskod 392 40 al al NNP cord-104231-fi8pskod 392 41 . . NNP cord-104231-fi8pskod 392 42 , , , cord-104231-fi8pskod 392 43 1992 1992 CD cord-104231-fi8pskod 392 44 ; ; : cord-104231-fi8pskod 392 45 Burke Burke NNP cord-104231-fi8pskod 392 46 et et NNP cord-104231-fi8pskod 392 47 al al NNP cord-104231-fi8pskod 392 48 . . NNP cord-104231-fi8pskod 392 49 , , , cord-104231-fi8pskod 392 50 1992 1992 CD cord-104231-fi8pskod 392 51 ; ; : cord-104231-fi8pskod 392 52 Colley Colley NNP cord-104231-fi8pskod 392 53 et et NNP cord-104231-fi8pskod 392 54 al al NNP cord-104231-fi8pskod 392 55 . . NNP cord-104231-fi8pskod 392 56 , , , cord-104231-fi8pskod 392 57 1992 1992 CD cord-104231-fi8pskod 393 1 ; ; : cord-104231-fi8pskod 393 2 Tang Tang NNP cord-104231-fi8pskod 393 3 et et NNP cord-104231-fi8pskod 393 4 ai ai NNP cord-104231-fi8pskod 393 5 . . NNP cord-104231-fi8pskod 393 6 , , , cord-104231-fi8pskod 393 7 1992 1992 CD cord-104231-fi8pskod 393 8 ; ; : cord-104231-fi8pskod 393 9 Teasdale Teasdale NNP cord-104231-fi8pskod 393 10 et et NNP cord-104231-fi8pskod 393 11 al al NNP cord-104231-fi8pskod 393 12 . . . cord-104231-fi8pskod 394 1 , , , cord-104231-fi8pskod 394 2 1992 1992 CD cord-104231-fi8pskod 394 3 ; ; : cord-104231-fi8pskod 394 4 Wong Wong NNP cord-104231-fi8pskod 394 5 et et NNP cord-104231-fi8pskod 394 6 al al NNP cord-104231-fi8pskod 394 7 . . NNP cord-104231-fi8pskod 394 8 , , , cord-104231-fi8pskod 394 9 1992 1992 CD cord-104231-fi8pskod 394 10 ) ) -RRB- cord-104231-fi8pskod 394 11 have have VBP cord-104231-fi8pskod 394 12 been be VBN cord-104231-fi8pskod 394 13 found find VBN cord-104231-fi8pskod 394 14 to to TO cord-104231-fi8pskod 394 15 be be VB cord-104231-fi8pskod 394 16 retained retain VBN cord-104231-fi8pskod 394 17 by by IN cord-104231-fi8pskod 394 18 the the DT cord-104231-fi8pskod 394 19 membrane membrane NN cord-104231-fi8pskod 394 20 - - HYPH cord-104231-fi8pskod 394 21 spanning span VBG cord-104231-fi8pskod 394 22 domain domain NN cord-104231-fi8pskod 394 23 . . . cord-104231-fi8pskod 395 1 The the DT cord-104231-fi8pskod 395 2 mechanism mechanism NN cord-104231-fi8pskod 395 3 of of IN cord-104231-fi8pskod 395 4 retention retention NN cord-104231-fi8pskod 395 5 is be VBZ cord-104231-fi8pskod 395 6 still still RB cord-104231-fi8pskod 395 7 unclear unclear JJ cord-104231-fi8pskod 395 8 though though IN cord-104231-fi8pskod 395 9 two two CD cord-104231-fi8pskod 395 10 possibilities possibility NNS cord-104231-fi8pskod 395 11 have have VBP cord-104231-fi8pskod 395 12 been be VBN cord-104231-fi8pskod 395 13 proposed propose VBN cord-104231-fi8pskod 395 14 . . . cord-104231-fi8pskod 396 1 TGN38 tgn38 NN cord-104231-fi8pskod 396 2 molecules molecule NNS cord-104231-fi8pskod 396 3 could could MD cord-104231-fi8pskod 396 4 interact interact VB cord-104231-fi8pskod 396 5 with with IN cord-104231-fi8pskod 396 6 their -PRON- PRP$ cord-104231-fi8pskod 396 7 neighbors neighbor NNS cord-104231-fi8pskod 396 8 through through IN cord-104231-fi8pskod 396 9 the the DT cord-104231-fi8pskod 396 10 spanning span VBG cord-104231-fi8pskod 396 11 domains domain NNS cord-104231-fi8pskod 396 12 forming form VBG cord-104231-fi8pskod 396 13 kin kin NN cord-104231-fi8pskod 396 14 oligomers oligomer NNS cord-104231-fi8pskod 396 15 so so RB cord-104231-fi8pskod 396 16 large large JJ cord-104231-fi8pskod 396 17 that that IN cord-104231-fi8pskod 396 18 they -PRON- PRP cord-104231-fi8pskod 396 19 can can MD cord-104231-fi8pskod 396 20 no no RB cord-104231-fi8pskod 396 21 longer longer RB cord-104231-fi8pskod 396 22 enter enter VB cord-104231-fi8pskod 396 23 vesicles vesicle NNS cord-104231-fi8pskod 396 24 budding bud VBG cord-104231-fi8pskod 396 25 from from IN cord-104231-fi8pskod 396 26 the the DT cord-104231-fi8pskod 396 27 TGN TGN NNP cord-104231-fi8pskod 396 28 ( ( -LRB- cord-104231-fi8pskod 396 29 Nilsson Nilsson NNP cord-104231-fi8pskod 396 30 et et NNP cord-104231-fi8pskod 396 31 al al NNP cord-104231-fi8pskod 396 32 . . NNP cord-104231-fi8pskod 396 33 , , , cord-104231-fi8pskod 396 34 1994 1994 CD cord-104231-fi8pskod 396 35 ) ) -RRB- cord-104231-fi8pskod 396 36 . . . cord-104231-fi8pskod 397 1 Alternatively alternatively RB cord-104231-fi8pskod 397 2 , , , cord-104231-fi8pskod 397 3 the the DT cord-104231-fi8pskod 397 4 length length NN cord-104231-fi8pskod 397 5 of of IN cord-104231-fi8pskod 397 6 the the DT cord-104231-fi8pskod 397 7 membrane membrane NN cord-104231-fi8pskod 397 8 - - HYPH cord-104231-fi8pskod 397 9 spanning span VBG cord-104231-fi8pskod 397 10 domain domain NN cord-104231-fi8pskod 397 11 might may MD cord-104231-fi8pskod 397 12 prevent prevent VB cord-104231-fi8pskod 397 13 forward forward JJ cord-104231-fi8pskod 397 14 movement movement NN cord-104231-fi8pskod 397 15 of of IN cord-104231-fi8pskod 397 16 the the DT cord-104231-fi8pskod 397 17 protein protein NN cord-104231-fi8pskod 397 18 past past IN cord-104231-fi8pskod 397 19 the the DT cord-104231-fi8pskod 397 20 TGN TGN NNP cord-104231-fi8pskod 397 21 ( ( -LRB- cord-104231-fi8pskod 397 22 Bretscher Bretscher NNP cord-104231-fi8pskod 397 23 and and CC cord-104231-fi8pskod 397 24 Munro Munro NNP cord-104231-fi8pskod 397 25 , , , cord-104231-fi8pskod 397 26 1993 1993 CD cord-104231-fi8pskod 397 27 ) ) -RRB- cord-104231-fi8pskod 397 28 . . . cord-104231-fi8pskod 398 1 The the DT cord-104231-fi8pskod 398 2 latter latter NN cord-104231-fi8pskod 398 3 seems seem VBZ cord-104231-fi8pskod 398 4 less less RBR cord-104231-fi8pskod 398 5 likely likely JJ cord-104231-fi8pskod 398 6 since since IN cord-104231-fi8pskod 398 7 the the DT cord-104231-fi8pskod 398 8 length length NN cord-104231-fi8pskod 398 9 of of IN cord-104231-fi8pskod 398 10 the the DT cord-104231-fi8pskod 398 11 membrane membrane NN cord-104231-fi8pskod 398 12 - - HYPH cord-104231-fi8pskod 398 13 spanning span VBG cord-104231-fi8pskod 398 14 domain domain NN cord-104231-fi8pskod 398 15 ( ( -LRB- cord-104231-fi8pskod 398 16 19 19 CD cord-104231-fi8pskod 398 17 amino amino JJ cord-104231-fi8pskod 398 18 acids acid NNS cord-104231-fi8pskod 398 19 ) ) -RRB- cord-104231-fi8pskod 398 20 is be VBZ cord-104231-fi8pskod 398 21 longer long JJR cord-104231-fi8pskod 398 22 than than IN cord-104231-fi8pskod 398 23 the the DT cord-104231-fi8pskod 398 24 estimated estimate VBN cord-104231-fi8pskod 398 25 average average NN cord-104231-fi8pskod 398 26 for for IN cord-104231-fi8pskod 398 27 Golgi Golgi NNP cord-104231-fi8pskod 398 28 proteins protein NNS cord-104231-fi8pskod 398 29 ( ( -LRB- cord-104231-fi8pskod 398 30 15 15 CD cord-104231-fi8pskod 398 31 amino amino JJ cord-104231-fi8pskod 398 32 acids acid NNS cord-104231-fi8pskod 398 33 ) ) -RRB- cord-104231-fi8pskod 398 34 but but CC cord-104231-fi8pskod 398 35 further further JJ cord-104231-fi8pskod 398 36 work work NN cord-104231-fi8pskod 398 37 will will MD cord-104231-fi8pskod 398 38 be be VB cord-104231-fi8pskod 398 39 needed need VBN cord-104231-fi8pskod 398 40 to to TO cord-104231-fi8pskod 398 41 determine determine VB cord-104231-fi8pskod 398 42 whether whether IN cord-104231-fi8pskod 398 43 either either DT cord-104231-fi8pskod 398 44 of of IN cord-104231-fi8pskod 398 45 these these DT cord-104231-fi8pskod 398 46 mechanisms mechanism NNS cord-104231-fi8pskod 398 47 , , , cord-104231-fi8pskod 398 48 or or CC cord-104231-fi8pskod 398 49 a a DT cord-104231-fi8pskod 398 50 completely completely RB cord-104231-fi8pskod 398 51 different different JJ cord-104231-fi8pskod 398 52 one one CD cord-104231-fi8pskod 398 53 , , , cord-104231-fi8pskod 398 54 are be VBP cord-104231-fi8pskod 398 55 responsible responsible JJ cord-104231-fi8pskod 398 56 for for IN cord-104231-fi8pskod 398 57 retention retention NN cord-104231-fi8pskod 398 58 in in IN cord-104231-fi8pskod 398 59 the the DT cord-104231-fi8pskod 398 60 TGN TGN NNP cord-104231-fi8pskod 398 61 . . . cord-104231-fi8pskod 399 1 Our -PRON- PRP$ cord-104231-fi8pskod 399 2 results result NNS cord-104231-fi8pskod 399 3 suggest suggest VBP cord-104231-fi8pskod 399 4 that that IN cord-104231-fi8pskod 399 5 the the DT cord-104231-fi8pskod 399 6 membrane membrane NN cord-104231-fi8pskod 399 7 - - HYPH cord-104231-fi8pskod 399 8 spanning span VBG cord-104231-fi8pskod 399 9 domain domain NN cord-104231-fi8pskod 399 10 is be VBZ cord-104231-fi8pskod 399 11 an an DT cord-104231-fi8pskod 399 12 effective effective JJ cord-104231-fi8pskod 399 13 retention retention NN cord-104231-fi8pskod 399 14 signal signal NN cord-104231-fi8pskod 399 15 since since IN cord-104231-fi8pskod 399 16 alone alone RB cord-104231-fi8pskod 399 17 it -PRON- PRP cord-104231-fi8pskod 399 18 can can MD cord-104231-fi8pskod 399 19 confer confer VB cord-104231-fi8pskod 399 20 localization localization NN cord-104231-fi8pskod 399 21 properties property NNS cord-104231-fi8pskod 399 22 on on IN cord-104231-fi8pskod 399 23 CD4 CD4 NNP cord-104231-fi8pskod 399 24 that that WDT cord-104231-fi8pskod 399 25 make make VBP cord-104231-fi8pskod 399 26 it -PRON- PRP cord-104231-fi8pskod 399 27 virtually virtually RB cord-104231-fi8pskod 399 28 indistinguishable indistinguishable JJ cord-104231-fi8pskod 399 29 from from IN cord-104231-fi8pskod 399 30 TGN38 TGN38 NNS cord-104231-fi8pskod 399 31 . . . cord-104231-fi8pskod 400 1 The the DT cord-104231-fi8pskod 400 2 predicted predict VBN cord-104231-fi8pskod 400 3 effect effect NN cord-104231-fi8pskod 400 4 of of IN cord-104231-fi8pskod 400 5 its -PRON- PRP$ cord-104231-fi8pskod 400 6 absence absence NN cord-104231-fi8pskod 400 7 would would MD cord-104231-fi8pskod 400 8 be be VB cord-104231-fi8pskod 400 9 an an DT cord-104231-fi8pskod 400 10 increased increase VBN cord-104231-fi8pskod 400 11 flux flux NN cord-104231-fi8pskod 400 12 of of IN cord-104231-fi8pskod 400 13 protein protein NN cord-104231-fi8pskod 400 14 through through IN cord-104231-fi8pskod 400 15 the the DT cord-104231-fi8pskod 400 16 TGN TGN NNP cord-104231-fi8pskod 400 17 to to IN cord-104231-fi8pskod 400 18 the the DT cord-104231-fi8pskod 400 19 cell cell NN cord-104231-fi8pskod 400 20 surface surface NN cord-104231-fi8pskod 400 21 . . . cord-104231-fi8pskod 401 1 In in IN cord-104231-fi8pskod 401 2 other other JJ cord-104231-fi8pskod 401 3 words word NNS cord-104231-fi8pskod 401 4 , , , cord-104231-fi8pskod 401 5 its -PRON- PRP$ cord-104231-fi8pskod 401 6 absence absence NN cord-104231-fi8pskod 401 7 should should MD cord-104231-fi8pskod 401 8 put put VB cord-104231-fi8pskod 401 9 a a DT cord-104231-fi8pskod 401 10 greatly greatly RB cord-104231-fi8pskod 401 11 increased increase VBN cord-104231-fi8pskod 401 12 load load NN cord-104231-fi8pskod 401 13 on on IN cord-104231-fi8pskod 401 14 to to IN cord-104231-fi8pskod 401 15 the the DT cord-104231-fi8pskod 401 16 retrieval retrieval NN cord-104231-fi8pskod 401 17 system system NN cord-104231-fi8pskod 401 18 . . . cord-104231-fi8pskod 402 1 As as RB cord-104231-fi8pskod 402 2 fast fast RB cord-104231-fi8pskod 402 3 as as IN cord-104231-fi8pskod 402 4 the the DT cord-104231-fi8pskod 402 5 protein protein NN cord-104231-fi8pskod 402 6 is be VBZ cord-104231-fi8pskod 402 7 retrieved retrieve VBN cord-104231-fi8pskod 402 8 and and CC cord-104231-fi8pskod 402 9 returned return VBN cord-104231-fi8pskod 402 10 to to IN cord-104231-fi8pskod 402 11 the the DT cord-104231-fi8pskod 402 12 TGN TGN NNP cord-104231-fi8pskod 402 13 , , , cord-104231-fi8pskod 402 14 it -PRON- PRP cord-104231-fi8pskod 402 15 would would MD cord-104231-fi8pskod 402 16 escape escape VB cord-104231-fi8pskod 402 17 to to IN cord-104231-fi8pskod 402 18 the the DT cord-104231-fi8pskod 402 19 plasma plasma NN cord-104231-fi8pskod 402 20 membrane membrane NN cord-104231-fi8pskod 402 21 and and CC cord-104231-fi8pskod 402 22 need need VBP cord-104231-fi8pskod 402 23 to to TO cord-104231-fi8pskod 402 24 be be VB cord-104231-fi8pskod 402 25 retrieved retrieve VBN cord-104231-fi8pskod 402 26 again again RB cord-104231-fi8pskod 402 27 . . . cord-104231-fi8pskod 403 1 This this DT cord-104231-fi8pskod 403 2 would would MD cord-104231-fi8pskod 403 3 explain explain VB cord-104231-fi8pskod 403 4 why why WRB cord-104231-fi8pskod 403 5 CD4-C CD4-C NNP cord-104231-fi8pskod 403 6 , , , cord-104231-fi8pskod 403 7 which which WDT cord-104231-fi8pskod 403 8 has have VBZ cord-104231-fi8pskod 403 9 the the DT cord-104231-fi8pskod 403 10 retrieval retrieval NN cord-104231-fi8pskod 403 11 but but CC cord-104231-fi8pskod 403 12 not not RB cord-104231-fi8pskod 403 13 the the DT cord-104231-fi8pskod 403 14 retention retention NN cord-104231-fi8pskod 403 15 signal signal NN cord-104231-fi8pskod 403 16 , , , cord-104231-fi8pskod 403 17 is be VBZ cord-104231-fi8pskod 403 18 present present JJ cord-104231-fi8pskod 403 19 in in IN cord-104231-fi8pskod 403 20 both both DT cord-104231-fi8pskod 403 21 endosomes endosome NNS cord-104231-fi8pskod 403 22 and and CC cord-104231-fi8pskod 403 23 at at IN cord-104231-fi8pskod 403 24 the the DT cord-104231-fi8pskod 403 25 plasma plasma NN cord-104231-fi8pskod 403 26 membrane membrane NN cord-104231-fi8pskod 403 27 even even RB cord-104231-fi8pskod 403 28 at at IN cord-104231-fi8pskod 403 29 low low JJ cord-104231-fi8pskod 403 30 levels level NNS cord-104231-fi8pskod 403 31 of of IN cord-104231-fi8pskod 403 32 expression expression NN cord-104231-fi8pskod 403 33 . . . cord-104231-fi8pskod 404 1 The the DT cord-104231-fi8pskod 404 2 rate rate NN cord-104231-fi8pskod 404 3 - - HYPH cord-104231-fi8pskod 404 4 limiting limit VBG cord-104231-fi8pskod 404 5 step step NN cord-104231-fi8pskod 404 6 in in IN cord-104231-fi8pskod 404 7 retrieval retrieval NN cord-104231-fi8pskod 404 8 is be VBZ cord-104231-fi8pskod 404 9 not not RB cord-104231-fi8pskod 404 10 endocytosis endocytosis NN cord-104231-fi8pskod 404 11 because because IN cord-104231-fi8pskod 404 12 the the DT cord-104231-fi8pskod 404 13 linear linear JJ cord-104231-fi8pskod 404 14 density density NN cord-104231-fi8pskod 404 15 of of IN cord-104231-fi8pskod 404 16 CD4-C CD4-C NNP cord-104231-fi8pskod 404 17 at at IN cord-104231-fi8pskod 404 18 the the DT cord-104231-fi8pskod 404 19 plasma plasma NN cord-104231-fi8pskod 404 20 membrane membrane NN cord-104231-fi8pskod 404 21 was be VBD cord-104231-fi8pskod 404 22 the the DT cord-104231-fi8pskod 404 23 same same JJ cord-104231-fi8pskod 404 24 at at IN cord-104231-fi8pskod 404 25 both both CC cord-104231-fi8pskod 404 26 low low JJ cord-104231-fi8pskod 404 27 and and CC cord-104231-fi8pskod 404 28 high high JJ cord-104231-fi8pskod 404 29 levels level NNS cord-104231-fi8pskod 404 30 of of IN cord-104231-fi8pskod 404 31 expression expression NN cord-104231-fi8pskod 404 32 . . . cord-104231-fi8pskod 405 1 The the DT cord-104231-fi8pskod 405 2 limiting limit VBG cord-104231-fi8pskod 405 3 step step NN cord-104231-fi8pskod 405 4 must must MD cord-104231-fi8pskod 405 5 be be VB cord-104231-fi8pskod 405 6 between between IN cord-104231-fi8pskod 405 7 endosomes endosome NNS cord-104231-fi8pskod 405 8 and and CC cord-104231-fi8pskod 405 9 the the DT cord-104231-fi8pskod 405 10 TGN TGN NNP cord-104231-fi8pskod 405 11 since since IN cord-104231-fi8pskod 405 12 CD4-C CD4-C NNP cord-104231-fi8pskod 405 13 accumulates accumulate VBZ cord-104231-fi8pskod 405 14 in in IN cord-104231-fi8pskod 405 15 endosomes endosome NNS cord-104231-fi8pskod 405 16 as as IN cord-104231-fi8pskod 405 17 the the DT cord-104231-fi8pskod 405 18 expression expression NN cord-104231-fi8pskod 405 19 level level NN cord-104231-fi8pskod 405 20 increases increase NNS cord-104231-fi8pskod 405 21 . . . cord-104231-fi8pskod 406 1 The the DT cord-104231-fi8pskod 406 2 only only JJ cord-104231-fi8pskod 406 3 result result NN cord-104231-fi8pskod 406 4 that that WDT cord-104231-fi8pskod 406 5 is be VBZ cord-104231-fi8pskod 406 6 a a DT cord-104231-fi8pskod 406 7 little little JJ cord-104231-fi8pskod 406 8 puzzling puzzling JJ cord-104231-fi8pskod 406 9 is be VBZ cord-104231-fi8pskod 406 10 the the DT cord-104231-fi8pskod 406 11 presence presence NN cord-104231-fi8pskod 406 12 of of IN cord-104231-fi8pskod 406 13 CD4-M CD4-M NNP cord-104231-fi8pskod 406 14 in in IN cord-104231-fi8pskod 406 15 endosomes endosome NNS cord-104231-fi8pskod 406 16 at at IN cord-104231-fi8pskod 406 17 high high JJ cord-104231-fi8pskod 406 18 expression expression NN cord-104231-fi8pskod 406 19 levels level NNS cord-104231-fi8pskod 406 20 . . . cord-104231-fi8pskod 407 1 This this DT cord-104231-fi8pskod 407 2 hybrid hybrid JJ cord-104231-fi8pskod 407 3 protein protein NN cord-104231-fi8pskod 407 4 would would MD cord-104231-fi8pskod 407 5 be be VB cord-104231-fi8pskod 407 6 expected expect VBN cord-104231-fi8pskod 407 7 to to TO cord-104231-fi8pskod 407 8 leak leak VB cord-104231-fi8pskod 407 9 very very RB cord-104231-fi8pskod 407 10 slowly slowly RB cord-104231-fi8pskod 407 11 to to IN cord-104231-fi8pskod 407 12 the the DT cord-104231-fi8pskod 407 13 cell cell NN cord-104231-fi8pskod 407 14 surface surface NN cord-104231-fi8pskod 407 15 , , , cord-104231-fi8pskod 407 16 but but CC cord-104231-fi8pskod 407 17 , , , cord-104231-fi8pskod 407 18 in in IN cord-104231-fi8pskod 407 19 the the DT cord-104231-fi8pskod 407 20 absence absence NN cord-104231-fi8pskod 407 21 of of IN cord-104231-fi8pskod 407 22 a a DT cord-104231-fi8pskod 407 23 retrieval retrieval NN cord-104231-fi8pskod 407 24 signal signal NN cord-104231-fi8pskod 407 25 , , , cord-104231-fi8pskod 407 26 it -PRON- PRP cord-104231-fi8pskod 407 27 should should MD cord-104231-fi8pskod 407 28 accumulate accumulate VB cord-104231-fi8pskod 407 29 there there RB cord-104231-fi8pskod 407 30 . . . cord-104231-fi8pskod 408 1 One one CD cord-104231-fi8pskod 408 2 possibility possibility NN cord-104231-fi8pskod 408 3 is be VBZ cord-104231-fi8pskod 408 4 that that IN cord-104231-fi8pskod 408 5 the the DT cord-104231-fi8pskod 408 6 endocytic endocytic JJ cord-104231-fi8pskod 408 7 signal signal NN cord-104231-fi8pskod 408 8 in in IN cord-104231-fi8pskod 408 9 the the DT cord-104231-fi8pskod 408 10 CD4 CD4 NNP cord-104231-fi8pskod 408 11 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 408 12 domain domain NN cord-104231-fi8pskod 408 13 ( ( -LRB- cord-104231-fi8pskod 408 14 Shin Shin NNP cord-104231-fi8pskod 408 15 et et FW cord-104231-fi8pskod 408 16 al al NNP cord-104231-fi8pskod 408 17 . . NNP cord-104231-fi8pskod 408 18 , , , cord-104231-fi8pskod 408 19 1991 1991 CD cord-104231-fi8pskod 408 20 ) ) -RRB- cord-104231-fi8pskod 408 21 triggers trigger VBZ cord-104231-fi8pskod 408 22 movement movement NN cord-104231-fi8pskod 408 23 to to IN cord-104231-fi8pskod 408 24 endosomes endosomes VB cord-104231-fi8pskod 408 25 ; ; : cord-104231-fi8pskod 408 26 another another DT cord-104231-fi8pskod 408 27 possibility possibility NN cord-104231-fi8pskod 408 28 is be VBZ cord-104231-fi8pskod 408 29 that that IN cord-104231-fi8pskod 408 30 the the DT cord-104231-fi8pskod 408 31 protein protein NN cord-104231-fi8pskod 408 32 in in IN cord-104231-fi8pskod 408 33 the the DT cord-104231-fi8pskod 408 34 endosome endosome NN cord-104231-fi8pskod 408 35 is be VBZ cord-104231-fi8pskod 408 36 destined destine VBN cord-104231-fi8pskod 408 37 for for IN cord-104231-fi8pskod 408 38 degradation degradation NN cord-104231-fi8pskod 408 39 in in IN cord-104231-fi8pskod 408 40 lysosomes lysosome NNS cord-104231-fi8pskod 408 41 . . . cord-104231-fi8pskod 409 1 This this DT cord-104231-fi8pskod 409 2 latter latter JJ cord-104231-fi8pskod 409 3 possibility possibility NN cord-104231-fi8pskod 409 4 is be VBZ cord-104231-fi8pskod 409 5 more more RBR cord-104231-fi8pskod 409 6 likely likely JJ cord-104231-fi8pskod 409 7 for for IN cord-104231-fi8pskod 409 8 CDS CDS NNP cord-104231-fi8pskod 409 9 - - HYPH cord-104231-fi8pskod 409 10 M M NNP cord-104231-fi8pskod 409 11 which which WDT cord-104231-fi8pskod 409 12 lacks lack VBZ cord-104231-fi8pskod 409 13 an an DT cord-104231-fi8pskod 409 14 endocytic endocytic JJ cord-104231-fi8pskod 409 15 signal signal NN cord-104231-fi8pskod 409 16 yet yet RB cord-104231-fi8pskod 409 17 is be VBZ cord-104231-fi8pskod 409 18 found find VBN cord-104231-fi8pskod 409 19 in in IN cord-104231-fi8pskod 409 20 small small JJ cord-104231-fi8pskod 409 21 amounts amount NNS cord-104231-fi8pskod 409 22 in in IN cord-104231-fi8pskod 409 23 what what WP cord-104231-fi8pskod 409 24 appear appear VBP cord-104231-fi8pskod 409 25 to to TO cord-104231-fi8pskod 409 26 be be VB cord-104231-fi8pskod 409 27 endosomes endosome NNS cord-104231-fi8pskod 409 28 by by IN cord-104231-fi8pskod 409 29 immunofluorescence immunofluorescence NN cord-104231-fi8pskod 409 30 microscopy microscopy NN cord-104231-fi8pskod 409 31 . . . cord-104231-fi8pskod 410 1 It -PRON- PRP cord-104231-fi8pskod 410 2 is be VBZ cord-104231-fi8pskod 410 3 likely likely JJ cord-104231-fi8pskod 410 4 that that IN cord-104231-fi8pskod 410 5 other other JJ cord-104231-fi8pskod 410 6 proteins protein NNS cord-104231-fi8pskod 410 7 on on IN cord-104231-fi8pskod 410 8 the the DT cord-104231-fi8pskod 410 9 exocytic exocytic JJ cord-104231-fi8pskod 410 10 pathway pathway NN cord-104231-fi8pskod 410 11 have have VBP cord-104231-fi8pskod 410 12 both both DT cord-104231-fi8pskod 410 13 retention retention NN cord-104231-fi8pskod 410 14 and and CC cord-104231-fi8pskod 410 15 retrieval retrieval NN cord-104231-fi8pskod 410 16 signals signal NNS cord-104231-fi8pskod 410 17 . . . cord-104231-fi8pskod 411 1 The the DT cord-104231-fi8pskod 411 2 sum sum NN cord-104231-fi8pskod 411 3 of of IN cord-104231-fi8pskod 411 4 the the DT cord-104231-fi8pskod 411 5 two two CD cord-104231-fi8pskod 411 6 processes process NNS cord-104231-fi8pskod 411 7 acting act VBG cord-104231-fi8pskod 411 8 on on IN cord-104231-fi8pskod 411 9 these these DT cord-104231-fi8pskod 411 10 signals signal NNS cord-104231-fi8pskod 411 11 would would MD cord-104231-fi8pskod 411 12 determine determine VB cord-104231-fi8pskod 411 13 the the DT cord-104231-fi8pskod 411 14 amount amount NN cord-104231-fi8pskod 411 15 of of IN cord-104231-fi8pskod 411 16 protein protein NN cord-104231-fi8pskod 411 17 found find VBN cord-104231-fi8pskod 411 18 in in IN cord-104231-fi8pskod 411 19 a a DT cord-104231-fi8pskod 411 20 particular particular JJ cord-104231-fi8pskod 411 21 compartment compartment NN cord-104231-fi8pskod 411 22 . . . cord-104231-fi8pskod 412 1 For for IN cord-104231-fi8pskod 412 2 resident resident JJ cord-104231-fi8pskod 412 3 proteins protein NNS cord-104231-fi8pskod 412 4 which which WDT cord-104231-fi8pskod 412 5 should should MD cord-104231-fi8pskod 412 6 spend spend VB cord-104231-fi8pskod 412 7 as as RB cord-104231-fi8pskod 412 8 much much JJ cord-104231-fi8pskod 412 9 of of IN cord-104231-fi8pskod 412 10 their -PRON- PRP$ cord-104231-fi8pskod 412 11 time time NN cord-104231-fi8pskod 412 12 as as IN cord-104231-fi8pskod 412 13 possible possible JJ cord-104231-fi8pskod 412 14 in in IN cord-104231-fi8pskod 412 15 a a DT cord-104231-fi8pskod 412 16 particular particular JJ cord-104231-fi8pskod 412 17 compartment compartment NN cord-104231-fi8pskod 412 18 one one PRP cord-104231-fi8pskod 412 19 would would MD cord-104231-fi8pskod 412 20 expect expect VB cord-104231-fi8pskod 412 21 retention retention NN cord-104231-fi8pskod 412 22 rather rather RB cord-104231-fi8pskod 412 23 than than IN cord-104231-fi8pskod 412 24 retrieval retrieval NN cord-104231-fi8pskod 412 25 to to TO cord-104231-fi8pskod 412 26 be be VB cord-104231-fi8pskod 412 27 the the DT cord-104231-fi8pskod 412 28 dominant dominant JJ cord-104231-fi8pskod 412 29 process process NN cord-104231-fi8pskod 412 30 . . . cord-104231-fi8pskod 413 1 For for IN cord-104231-fi8pskod 413 2 recycling recycling NN cord-104231-fi8pskod 413 3 proteins protein NNS cord-104231-fi8pskod 413 4 , , , cord-104231-fi8pskod 413 5 such such JJ cord-104231-fi8pskod 413 6 as as IN cord-104231-fi8pskod 413 7 receptors receptor NNS cord-104231-fi8pskod 413 8 , , , cord-104231-fi8pskod 413 9 one one PRP cord-104231-fi8pskod 413 10 would would MD cord-104231-fi8pskod 413 11 expect expect VB cord-104231-fi8pskod 413 12 the the DT cord-104231-fi8pskod 413 13 opposite opposite NN cord-104231-fi8pskod 413 14 so so IN cord-104231-fi8pskod 413 15 that that IN cord-104231-fi8pskod 413 16 substantial substantial JJ cord-104231-fi8pskod 413 17 amounts amount NNS cord-104231-fi8pskod 413 18 of of IN cord-104231-fi8pskod 413 19 protein protein NN cord-104231-fi8pskod 413 20 would would MD cord-104231-fi8pskod 413 21 be be VB cord-104231-fi8pskod 413 22 moving move VBG cord-104231-fi8pskod 413 23 between between IN cord-104231-fi8pskod 413 24 two two CD cord-104231-fi8pskod 413 25 compartments compartment NNS cord-104231-fi8pskod 413 26 . . . cord-104231-fi8pskod 414 1 It -PRON- PRP cord-104231-fi8pskod 414 2 is be VBZ cord-104231-fi8pskod 414 3 not not RB cord-104231-fi8pskod 414 4 yet yet RB cord-104231-fi8pskod 414 5 clear clear JJ cord-104231-fi8pskod 414 6 whether whether IN cord-104231-fi8pskod 414 7 TGN38 TGN38 NNP cord-104231-fi8pskod 414 8 is be VBZ cord-104231-fi8pskod 414 9 best well RBS cord-104231-fi8pskod 414 10 considered consider VBN cord-104231-fi8pskod 414 11 as as IN cord-104231-fi8pskod 414 12 a a DT cord-104231-fi8pskod 414 13 resident resident NN cord-104231-fi8pskod 414 14 or or CC cord-104231-fi8pskod 414 15 a a DT cord-104231-fi8pskod 414 16 recycling recycling NN cord-104231-fi8pskod 414 17 protein protein NN cord-104231-fi8pskod 414 18 ( ( -LRB- cord-104231-fi8pskod 414 19 Luzio Luzio NNP cord-104231-fi8pskod 414 20 and and CC cord-104231-fi8pskod 414 21 Banting Banting NNP cord-104231-fi8pskod 414 22 , , , cord-104231-fi8pskod 414 23 1993 1993 CD cord-104231-fi8pskod 414 24 ; ; : cord-104231-fi8pskod 414 25 Stanley Stanley NNP cord-104231-fi8pskod 414 26 and and CC cord-104231-fi8pskod 414 27 Howell Howell NNP cord-104231-fi8pskod 414 28 , , , cord-104231-fi8pskod 414 29 1993 1993 CD cord-104231-fi8pskod 414 30 ) ) -RRB- cord-104231-fi8pskod 414 31 . . . cord-104231-fi8pskod 415 1 That that DT cord-104231-fi8pskod 415 2 will will MD cord-104231-fi8pskod 415 3 have have VB cord-104231-fi8pskod 415 4 to to TO cord-104231-fi8pskod 415 5 await await VB cord-104231-fi8pskod 415 6 discovery discovery NN cord-104231-fi8pskod 415 7 of of IN cord-104231-fi8pskod 415 8 its -PRON- PRP$ cord-104231-fi8pskod 415 9 function function NN cord-104231-fi8pskod 415 10 . . . cord-104231-fi8pskod 416 1 Golgi Golgi NNP cord-104231-fi8pskod 416 2 retention retention NN cord-104231-fi8pskod 416 3 of of IN cord-104231-fi8pskod 416 4 a a DT cord-104231-fi8pskod 416 5 trans trans JJ cord-104231-fi8pskod 416 6 - - JJ cord-104231-fi8pskod 416 7 Golgi Golgi NNP cord-104231-fi8pskod 416 8 membrane membrane NN cord-104231-fi8pskod 416 9 protein protein NN cord-104231-fi8pskod 416 10 , , , cord-104231-fi8pskod 416 11 galactosyltransferase galactosyltransferase NNP cord-104231-fi8pskod 416 12 , , , cord-104231-fi8pskod 416 13 requires require VBZ cord-104231-fi8pskod 416 14 cysteine cysteine NN cord-104231-fi8pskod 416 15 and and CC cord-104231-fi8pskod 416 16 histidiue histidiue JJ cord-104231-fi8pskod 416 17 residues residue NNS cord-104231-fi8pskod 416 18 within within IN cord-104231-fi8pskod 416 19 the the DT cord-104231-fi8pskod 416 20 membrane membrane NN cord-104231-fi8pskod 416 21 - - HYPH cord-104231-fi8pskod 416 22 anchoring anchor VBG cord-104231-fi8pskod 416 23 domain domain NN cord-104231-fi8pskod 416 24 Monoclonal Monoclonal NNP cord-104231-fi8pskod 416 25 antibodies antibody NNS cord-104231-fi8pskod 416 26 to to IN cord-104231-fi8pskod 416 27 soluble soluble JJ cord-104231-fi8pskod 416 28 , , , cord-104231-fi8pskod 416 29 human human JJ cord-104231-fi8pskod 416 30 milk milk NN cord-104231-fi8pskod 416 31 galactosyltransferase galactosyltransferase NNP cord-104231-fi8pskod 416 32 0actose 0actose . cord-104231-fi8pskod 416 33 synthase synthase NN cord-104231-fi8pskod 416 34 A a DT cord-104231-fi8pskod 416 35 protein protein NN cord-104231-fi8pskod 416 36 ) ) -RRB- cord-104231-fi8pskod 417 1 TGN38 TGN38 NNP cord-104231-fi8pskod 417 2 is be VBZ cord-104231-fi8pskod 417 3 maintained maintain VBN cord-104231-fi8pskod 417 4 in in IN cord-104231-fi8pskod 417 5 the the DT cord-104231-fi8pskod 417 6 trans trans NNP cord-104231-fi8pskod 417 7 - - NNP cord-104231-fi8pskod 417 8 Goigi Goigi NNP cord-104231-fi8pskod 417 9 network network NN cord-104231-fi8pskod 417 10 by by IN cord-104231-fi8pskod 417 11 a a DT cord-104231-fi8pskod 417 12 tyrosine tyrosine NN cord-104231-fi8pskod 417 13 - - HYPH cord-104231-fi8pskod 417 14 containing contain VBG cord-104231-fi8pskod 417 15 motif motif NN cord-104231-fi8pskod 417 16 in in IN cord-104231-fi8pskod 417 17 the the DT cord-104231-fi8pskod 417 18 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 417 19 domain domain NN cord-104231-fi8pskod 417 20 Evidence evidence NN cord-104231-fi8pskod 417 21 for for IN cord-104231-fi8pskod 417 22 an an DT cord-104231-fi8pskod 417 23 extended extended JJ cord-104231-fi8pskod 417 24 of of IN cord-104231-fi8pskod 417 25 the the DT cord-104231-fi8pskod 417 26 T t NN cord-104231-fi8pskod 417 27 - - HYPH cord-104231-fi8pskod 417 28 cell cell NN cord-104231-fi8pskod 417 29 co co NN cord-104231-fi8pskod 417 30 - - NN cord-104231-fi8pskod 417 31 receptor receptor NN cord-104231-fi8pskod 417 32 CDga CDga NNP cord-104231-fi8pskod 417 33 as as IN cord-104231-fi8pskod 417 34 deduced deduce VBN cord-104231-fi8pskod 417 35 from from IN cord-104231-fi8pskod 417 36 the the DT cord-104231-fi8pskod 417 37 hydrodynamic hydrodynamic JJ cord-104231-fi8pskod 417 38 properties property NNS cord-104231-fi8pskod 417 39 of of IN cord-104231-fi8pskod 417 40 soluble soluble JJ cord-104231-fi8pskod 417 41 forms form NNS cord-104231-fi8pskod 417 42 of of IN cord-104231-fi8pskod 417 43 the the DT cord-104231-fi8pskod 417 44 extracellular extracellular JJ cord-104231-fi8pskod 417 45 region region NN cord-104231-fi8pskod 417 46 Cholesterol cholesterol NN cord-104231-fi8pskod 417 47 and and CC cord-104231-fi8pskod 417 48 the the DT cord-104231-fi8pskod 417 49 Golgi Golgi NNP cord-104231-fi8pskod 417 50 apparatus apparatus NN cord-104231-fi8pskod 417 51 The the DT cord-104231-fi8pskod 417 52 transmembrane transmembrane NN cord-104231-fi8pskod 417 53 and and CC cord-104231-fi8pskod 417 54 flanking flanking JJ cord-104231-fi8pskod 417 55 sequences sequence NNS cord-104231-fi8pskod 417 56 of of IN cord-104231-fi8pskod 417 57 fll,2-N fll,2-n JJ cord-104231-fi8pskod 417 58 - - HYPH cord-104231-fi8pskod 417 59 acetylglucosaminyltransferase acetylglucosaminyltransferase NN cord-104231-fi8pskod 417 60 I -PRON- PRP cord-104231-fi8pskod 417 61 specify specify VBP cord-104231-fi8pskod 417 62 medial medial JJ cord-104231-fi8pskod 417 63 - - HYPH cord-104231-fi8pskod 417 64 Goigi goigi JJ cord-104231-fi8pskod 417 65 localization localization NN cord-104231-fi8pskod 418 1 Transferrin Transferrin NNP cord-104231-fi8pskod 418 2 receptor receptor NN cord-104231-fi8pskod 418 3 internalization internalization NN cord-104231-fi8pskod 418 4 sequence sequence NN cord-104231-fi8pskod 419 1 YXRF YXRF NNP cord-104231-fi8pskod 419 2 implicates implicate VBZ cord-104231-fi8pskod 419 3 a a DT cord-104231-fi8pskod 419 4 tight tight JJ cord-104231-fi8pskod 419 5 turn turn NN cord-104231-fi8pskod 419 6 as as IN cord-104231-fi8pskod 419 7 the the DT cord-104231-fi8pskod 419 8 structural structural JJ cord-104231-fi8pskod 419 9 recognition recognition NN cord-104231-fi8pskod 419 10 motif motif NN cord-104231-fi8pskod 419 11 for for IN cord-104231-fi8pskod 419 12 endocytosis endocytosis NN cord-104231-fi8pskod 420 1 The the DT cord-104231-fi8pskod 420 2 signal signal NN cord-104231-fi8pskod 420 3 anchor anchor NN cord-104231-fi8pskod 420 4 and and CC cord-104231-fi8pskod 420 5 stem stem NN cord-104231-fi8pskod 420 6 regions region NNS cord-104231-fi8pskod 420 7 of of IN cord-104231-fi8pskod 420 8 the the DT cord-104231-fi8pskod 420 9 fl fl NNP cord-104231-fi8pskod 420 10 - - HYPH cord-104231-fi8pskod 420 11 galactoside galactoside NNP cord-104231-fi8pskod 420 12 ot2,6-sialyltransferase ot2,6-sialyltransferase CD cord-104231-fi8pskod 421 1 may may MD cord-104231-fi8pskod 421 2 each each DT cord-104231-fi8pskod 421 3 act act VB cord-104231-fi8pskod 421 4 to to TO cord-104231-fi8pskod 421 5 localize localize VB cord-104231-fi8pskod 421 6 the the DT cord-104231-fi8pskod 421 7 enzyme enzyme NN cord-104231-fi8pskod 421 8 to to IN cord-104231-fi8pskod 421 9 the the DT cord-104231-fi8pskod 421 10 Golgi Golgi NNP cord-104231-fi8pskod 421 11 apparatus apparatus NN cord-104231-fi8pskod 422 1 Tubulovesicular Tubulovesicular NNP cord-104231-fi8pskod 422 2 processes process NNS cord-104231-fi8pskod 422 3 emerge emerge VBP cord-104231-fi8pskod 422 4 from from IN cord-104231-fi8pskod 422 5 tmas tmas NNP cord-104231-fi8pskod 422 6 - - HYPH cord-104231-fi8pskod 422 7 Golgi Golgi NNP cord-104231-fi8pskod 422 8 cisteroae cisteroae NNP cord-104231-fi8pskod 422 9 , , , cord-104231-fi8pskod 422 10 extend extend VB cord-104231-fi8pskod 422 11 along along IN cord-104231-fi8pskod 422 12 microtubules microtubule NNS cord-104231-fi8pskod 422 13 , , , cord-104231-fi8pskod 422 14 and and CC cord-104231-fi8pskod 422 15 interlink interlink VB cord-104231-fi8pskod 422 16 adjacent adjacent JJ cord-104231-fi8pskod 422 17 trans trans NNP cord-104231-fi8pskod 422 18 - - NNP cord-104231-fi8pskod 422 19 Golgi Golgi NNP cord-104231-fi8pskod 422 20 elements element NNS cord-104231-fi8pskod 422 21 into into IN cord-104231-fi8pskod 422 22 a a DT cord-104231-fi8pskod 422 23 reticulum reticulum NN cord-104231-fi8pskod 422 24 Soluble soluble JJ cord-104231-fi8pskod 422 25 form form NN cord-104231-fi8pskod 422 26 of of IN cord-104231-fi8pskod 422 27 CD4 CD4 NNP cord-104231-fi8pskod 422 28 ( ( -LRB- cord-104231-fi8pskod 422 29 ' ' `` cord-104231-fi8pskod 422 30 I"4 I"4 NNP cord-104231-fi8pskod 422 31 ) ) -RRB- cord-104231-fi8pskod 422 32 protein protein NN cord-104231-fi8pskod 422 33 inhibits inhibit VBZ cord-104231-fi8pskod 422 34 AIDS AIDS NNP cord-104231-fi8pskod 422 35 virus virus NN cord-104231-fi8pskod 422 36 infection infection NN cord-104231-fi8pskod 422 37 Protein Protein NNP cord-104231-fi8pskod 422 38 folding fold VBG cord-104231-fi8pskod 422 39 in in IN cord-104231-fi8pskod 422 40 the the DT cord-104231-fi8pskod 422 41 cell cell NN cord-104231-fi8pskod 423 1 Intracellular Intracellular NNP cord-104231-fi8pskod 423 2 site site NN cord-104231-fi8pskod 423 3 of of IN cord-104231-fi8pskod 423 4 aalaloglycoprotein aalaloglycoprotein NNP cord-104231-fi8pskod 423 5 receptor receptor NN cord-104231-fi8pskod 423 6 - - HYPH cord-104231-fi8pskod 423 7 ligand ligand NN cord-104231-fi8pskod 423 8 uncoupling uncoupling NN cord-104231-fi8pskod 423 9 : : : cord-104231-fi8pskod 424 1 doublelabel doublelabel NNP cord-104231-fi8pskod 424 2 immunoelectron immunoelectron NNP cord-104231-fi8pskod 424 3 microscopy microscopy NNP cord-104231-fi8pskod 424 4 during during IN cord-104231-fi8pskod 424 5 receptor receptor NN cord-104231-fi8pskod 424 6 mediated mediate VBN cord-104231-fi8pskod 424 7 endocytosis endocytosis NN cord-104231-fi8pskod 424 8 Sorting sorting NN cord-104231-fi8pskod 424 9 of of IN cord-104231-fi8pskod 424 10 mannose mannose NN cord-104231-fi8pskod 424 11 6-phosphate 6-phosphate NN cord-104231-fi8pskod 424 12 receptors receptor NNS cord-104231-fi8pskod 424 13 and and CC cord-104231-fi8pskod 424 14 lysosomal lysosomal JJ cord-104231-fi8pskod 424 15 membrane membrane NN cord-104231-fi8pskod 424 16 proteins protein NNS cord-104231-fi8pskod 424 17 in in IN cord-104231-fi8pskod 424 18 endocytic endocytic JJ cord-104231-fi8pskod 424 19 vesicles vesicle NNS cord-104231-fi8pskod 425 1 The the DT cord-104231-fi8pskod 425 2 trans trans NNP cord-104231-fi8pskod 425 3 Golgi Golgi NNP cord-104231-fi8pskod 425 4 network network NN cord-104231-fi8pskod 425 5 : : : cord-104231-fi8pskod 425 6 sorting sort VBG cord-104231-fi8pskod 425 7 at at IN cord-104231-fi8pskod 425 8 the the DT cord-104231-fi8pskod 425 9 exit exit NN cord-104231-fi8pskod 425 10 site site NN cord-104231-fi8pskod 425 11 of of IN cord-104231-fi8pskod 425 12 the the DT cord-104231-fi8pskod 425 13 Golgi Golgi NNP cord-104231-fi8pskod 425 14 complex complex NN cord-104231-fi8pskod 426 1 The the DT cord-104231-fi8pskod 426 2 dynamic dynamic JJ cord-104231-fi8pskod 426 3 nature nature NN cord-104231-fi8pskod 426 4 of of IN cord-104231-fi8pskod 426 5 the the DT cord-104231-fi8pskod 426 6 Golgi Golgi NNP cord-104231-fi8pskod 426 7 complex complex NN cord-104231-fi8pskod 426 8 Summary summary NN cord-104231-fi8pskod 426 9 of of IN cord-104231-fi8pskod 426 10 T T NNP cord-104231-fi8pskod 426 11 cell cell NN cord-104231-fi8pskod 426 12 studies study NNS cord-104231-fi8pskod 426 13 performed perform VBN cord-104231-fi8pskod 426 14 during during IN cord-104231-fi8pskod 426 15 the the DT cord-104231-fi8pskod 426 16 second second JJ cord-104231-fi8pskod 426 17 international international JJ cord-104231-fi8pskod 426 18 workshop workshop NN cord-104231-fi8pskod 426 19 and and CC cord-104231-fi8pskod 426 20 conference conference NN cord-104231-fi8pskod 426 21 on on IN cord-104231-fi8pskod 426 22 buman buman NNP cord-104231-fi8pskod 426 23 leukocyte leukocyte NN cord-104231-fi8pskod 426 24 differentiation differentiation NN cord-104231-fi8pskod 426 25 antigens antigen VBD cord-104231-fi8pskod 426 26 Novel Novel NNP cord-104231-fi8pskod 426 27 anti anti JJ cord-104231-fi8pskod 426 28 - - JJ cord-104231-fi8pskod 426 29 CD4 cd4 JJ cord-104231-fi8pskod 426 30 monoclonal monoclonal JJ cord-104231-fi8pskod 426 31 antibodies antibody NNS cord-104231-fi8pskod 426 32 separate separate VBP cord-104231-fi8pskod 426 33 human human JJ cord-104231-fi8pskod 426 34 immunodeficiency immunodeficiency NN cord-104231-fi8pskod 426 35 virus virus NN cord-104231-fi8pskod 426 36 infection infection NN cord-104231-fi8pskod 426 37 and and CC cord-104231-fi8pskod 426 38 fusion fusion NN cord-104231-fi8pskod 426 39 of of IN cord-104231-fi8pskod 426 40 CD4 CD4 NNP cord-104231-fi8pskod 426 41 + + CC cord-104231-fi8pskod 426 42 cells cell NNS cord-104231-fi8pskod 426 43 from from IN cord-104231-fi8pskod 426 44 virus virus NN cord-104231-fi8pskod 426 45 binding bind VBG cord-104231-fi8pskod 426 46 Movement Movement NNP cord-104231-fi8pskod 426 47 of of IN cord-104231-fi8pskod 426 48 internalized internalize VBN cord-104231-fi8pskod 426 49 ligand ligand NN cord-104231-fi8pskod 426 50 - - HYPH cord-104231-fi8pskod 426 51 receptor receptor NN cord-104231-fi8pskod 426 52 complexes complex NNS cord-104231-fi8pskod 426 53 along along IN cord-104231-fi8pskod 426 54 a a DT cord-104231-fi8pskod 426 55 continuous continuous JJ cord-104231-fi8pskod 426 56 endosomal endosomal NNP cord-104231-fi8pskod 426 57 reticuinm reticuinm NNP cord-104231-fi8pskod 426 58 Localization Localization NNP cord-104231-fi8pskod 426 59 of of IN cord-104231-fi8pskod 426 60 TGN38 TGN38 NNP cord-104231-fi8pskod 426 61 to to IN cord-104231-fi8pskod 426 62 the the DT cord-104231-fi8pskod 426 63 trans trans NNP cord-104231-fi8pskod 426 64 - - NNP cord-104231-fi8pskod 426 65 Golo Golo NNP cord-104231-fi8pskod 426 66 network network NN cord-104231-fi8pskod 426 67 : : : cord-104231-fi8pskod 426 68 involvement involvement NN cord-104231-fi8pskod 426 69 of of IN cord-104231-fi8pskod 426 70 a a DT cord-104231-fi8pskod 426 71 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 426 72 tyrosine tyrosine NN cord-104231-fi8pskod 426 73 - - HYPH cord-104231-fi8pskod 426 74 contalning contalne VBG cord-104231-fi8pskod 426 75 sequence sequence NN cord-104231-fi8pskod 426 76 Identification identification NN cord-104231-fi8pskod 426 77 of of IN cord-104231-fi8pskod 426 78 a a DT cord-104231-fi8pskod 426 79 consensus consensus NN cord-104231-fi8pskod 426 80 motif motif NN cord-104231-fi8pskod 426 81 for for IN cord-104231-fi8pskod 426 82 retention retention NN cord-104231-fi8pskod 426 83 of of IN cord-104231-fi8pskod 426 84 transmembrane transmembrane JJ cord-104231-fi8pskod 426 85 proteins protein NNS cord-104231-fi8pskod 426 86 in in IN cord-104231-fi8pskod 426 87 the the DT cord-104231-fi8pskod 426 88 endoplasmic endoplasmic NN cord-104231-fi8pskod 426 89 reticulum reticulum NN cord-104231-fi8pskod 426 90 Retrieval Retrieval NNP cord-104231-fi8pskod 426 91 of of IN cord-104231-fi8pskod 426 92 transmembraue transmembraue NNP cord-104231-fi8pskod 426 93 proteins protein NNS cord-104231-fi8pskod 426 94 to to IN cord-104231-fi8pskod 426 95 the the DT cord-104231-fi8pskod 426 96 endoplasmic endoplasmic NN cord-104231-fi8pskod 426 97 - - HYPH cord-104231-fi8pskod 426 98 reticulum reticulum NN cord-104231-fi8pskod 426 99 A a DT cord-104231-fi8pskod 426 100 cytosolic cytosolic JJ cord-104231-fi8pskod 426 101 complex complex NN cord-104231-fi8pskod 426 102 of of IN cord-104231-fi8pskod 426 103 p62 p62 NN cord-104231-fi8pskod 426 104 and and CC cord-104231-fi8pskod 426 105 rab6 rab6 NNP cord-104231-fi8pskod 426 106 associates associate NNS cord-104231-fi8pskod 426 107 with with IN cord-104231-fi8pskod 426 108 TGN38/41 TGN38/41 NNP cord-104231-fi8pskod 426 109 and and CC cord-104231-fi8pskod 426 110 is be VBZ cord-104231-fi8pskod 426 111 involved involve VBN cord-104231-fi8pskod 426 112 in in IN cord-104231-fi8pskod 426 113 budding bud VBG cord-104231-fi8pskod 426 114 of of IN cord-104231-fi8pskod 426 115 exocytic exocytic JJ cord-104231-fi8pskod 426 116 vesicles vesicle NNS cord-104231-fi8pskod 426 117 from from IN cord-104231-fi8pskod 426 118 the the DT cord-104231-fi8pskod 426 119 trans trans NNP cord-104231-fi8pskod 426 120 - - NNP cord-104231-fi8pskod 426 121 Golgi Golgi NNP cord-104231-fi8pskod 426 122 network network NN cord-104231-fi8pskod 426 123 Differences difference NNS cord-104231-fi8pskod 426 124 in in IN cord-104231-fi8pskod 426 125 the the DT cord-104231-fi8pskod 426 126 endosomal endosomal NNP cord-104231-fi8pskod 426 127 distributions distribution NNS cord-104231-fi8pskod 426 128 of of IN cord-104231-fi8pskod 426 129 the the DT cord-104231-fi8pskod 426 130 two two CD cord-104231-fi8pskod 426 131 mannose-6-phosphate mannose-6-phosphate NNP cord-104231-fi8pskod 426 132 receptors receptor NNS cord-104231-fi8pskod 426 133 Structure structure NN cord-104231-fi8pskod 426 134 and and CC cord-104231-fi8pskod 426 135 function function NN cord-104231-fi8pskod 426 136 of of IN cord-104231-fi8pskod 426 137 the the DT cord-104231-fi8pskod 426 138 mannose mannose NN cord-104231-fi8pskod 426 139 6-phosphate 6-phosphate NNP cord-104231-fi8pskod 426 140 / / SYM cord-104231-fi8pskod 426 141 insulinlike insulinlike JJ cord-104231-fi8pskod 426 142 growth growth NN cord-104231-fi8pskod 426 143 factor factor NN cord-104231-fi8pskod 426 144 II II NNP cord-104231-fi8pskod 426 145 receptors receptor NNS cord-104231-fi8pskod 426 146 Cry.stal cry.stal DT cord-104231-fi8pskod 426 147 structure structure NN cord-104231-fi8pskod 426 148 of of IN cord-104231-fi8pskod 426 149 a a DT cord-104231-fi8pskod 426 150 soluble soluble JJ cord-104231-fi8pskod 426 151 form form NN cord-104231-fi8pskod 426 152 of of IN cord-104231-fi8pskod 426 153 the the DT cord-104231-fi8pskod 426 154 human human JJ cord-104231-fi8pskod 426 155 T T NNP cord-104231-fi8pskod 426 156 cell cell NN cord-104231-fi8pskod 426 157 coreceptor coreceptor NN cord-104231-fi8pskod 426 158 CD8 CD8 NNP cord-104231-fi8pskod 426 159 at at IN cord-104231-fi8pskod 426 160 2.6 2.6 CD cord-104231-fi8pskod 426 161 A a DT cord-104231-fi8pskod 426 162 resolution resolution NN cord-104231-fi8pskod 426 163 Ligand ligand NN cord-104231-fi8pskod 426 164 - - HYPH cord-104231-fi8pskod 426 165 induced induce VBN cord-104231-fi8pskod 426 166 redistribution redistribution NN cord-104231-fi8pskod 426 167 of of IN cord-104231-fi8pskod 426 168 a a DT cord-104231-fi8pskod 426 169 human human JJ cord-104231-fi8pskod 426 170 KDEL KDEL NNP cord-104231-fi8pskod 426 171 receptor receptor NN cord-104231-fi8pskod 426 172 from from IN cord-104231-fi8pskod 426 173 the the DT cord-104231-fi8pskod 426 174 Golgi Golgi NNP cord-104231-fi8pskod 426 175 complex complex NN cord-104231-fi8pskod 426 176 to to IN cord-104231-fi8pskod 426 177 the the DT cord-104231-fi8pskod 426 178 endoplasmic endoplasmic NN cord-104231-fi8pskod 426 179 reticulum reticulum NN cord-104231-fi8pskod 427 1 The the DT cord-104231-fi8pskod 427 2 isolation isolation NN cord-104231-fi8pskod 427 3 and and CC cord-104231-fi8pskod 427 4 sequence sequence NN cord-104231-fi8pskod 427 5 of of IN cord-104231-fi8pskod 427 6 the the DT cord-104231-fi8pskod 427 7 gene gene NN cord-104231-fi8pskod 427 8 encoding encode VBG cord-104231-fi8pskod 427 9 T8 T8 NNP cord-104231-fi8pskod 427 10 : : : cord-104231-fi8pskod 427 11 a a DT cord-104231-fi8pskod 427 12 molecule molecule NN cord-104231-fi8pskod 427 13 defining define VBG cord-104231-fi8pskod 427 14 functional functional JJ cord-104231-fi8pskod 427 15 classes class NNS cord-104231-fi8pskod 427 16 of of IN cord-104231-fi8pskod 427 17 T T NNP cord-104231-fi8pskod 427 18 lymphccytes lymphccyte NNS cord-104231-fi8pskod 427 19 O o NN cord-104231-fi8pskod 427 20 - - HYPH cord-104231-fi8pskod 427 21 glycosylation glycosylation NN cord-104231-fi8pskod 427 22 of of IN cord-104231-fi8pskod 427 23 the the DT cord-104231-fi8pskod 427 24 coronavirus coronavirus NN cord-104231-fi8pskod 427 25 M M NNP cord-104231-fi8pskod 427 26 protein protein NN cord-104231-fi8pskod 427 27 . . . cord-104231-fi8pskod 428 1 Differential differential JJ cord-104231-fi8pskod 428 2 localization localization NN cord-104231-fi8pskod 428 3 of of IN cord-104231-fi8pskod 428 4 sialyltransferases sialyltransferase NNS cord-104231-fi8pskod 428 5 in in IN cord-104231-fi8pskod 428 6 N N NNP cord-104231-fi8pskod 428 7 - - : cord-104231-fi8pskod 428 8 and and CC cord-104231-fi8pskod 428 9 O o NN cord-104231-fi8pskod 428 10 - - HYPH cord-104231-fi8pskod 428 11 linked link VBN cord-104231-fi8pskod 428 12 glycosylation glycosylation NN cord-104231-fi8pskod 428 13 Mitotic mitotic NN cord-104231-fi8pskod 428 14 Golgi Golgi NNP cord-104231-fi8pskod 428 15 fragments fragment NNS cord-104231-fi8pskod 428 16 in in IN cord-104231-fi8pskod 428 17 HeLe HeLe NNP cord-104231-fi8pskod 428 18 cells cell NNS cord-104231-fi8pskod 428 19 and and CC cord-104231-fi8pskod 428 20 their -PRON- PRP$ cord-104231-fi8pskod 428 21 role role NN cord-104231-fi8pskod 428 22 in in IN cord-104231-fi8pskod 428 23 the the DT cord-104231-fi8pskod 428 24 re re NN cord-104231-fi8pskod 428 25 , , , cord-104231-fi8pskod 428 26 assembly assembly NN cord-104231-fi8pskod 428 27 pathway pathway NN cord-104231-fi8pskod 428 28 Eukaryote Eukaryote NNP cord-104231-fi8pskod 428 29 membrane membrane NN cord-104231-fi8pskod 428 30 traffic traffic NN cord-104231-fi8pskod 428 31 - - HYPH cord-104231-fi8pskod 428 32 retrieval retrieval NN cord-104231-fi8pskod 428 33 and and CC cord-104231-fi8pskod 428 34 retention retention NN cord-104231-fi8pskod 428 35 to to TO cord-104231-fi8pskod 428 36 achieve achieve VB cord-104231-fi8pskod 428 37 organelle organelle NN cord-104231-fi8pskod 428 38 residence residence NN cord-104231-fi8pskod 428 39 Identification identification NN cord-104231-fi8pskod 428 40 , , , cord-104231-fi8pskod 428 41 sequencing sequencing NN cord-104231-fi8pskod 428 42 and and CC cord-104231-fi8pskod 428 43 expression expression NN cord-104231-fi8pskod 428 44 of of IN cord-104231-fi8pskod 428 45 an an DT cord-104231-fi8pskod 428 46 integral integral JJ cord-104231-fi8pskod 428 47 membrane membrane NN cord-104231-fi8pskod 428 48 protein protein NN cord-104231-fi8pskod 428 49 of of IN cord-104231-fi8pskod 428 50 the the DT cord-104231-fi8pskod 428 51 trans trans NNP cord-104231-fi8pskod 428 52 - - NNP cord-104231-fi8pskod 428 53 Golgi Golgi NNP cord-104231-fi8pskod 428 54 network network NN cord-104231-fi8pskod 428 55 ( ( -LRB- cord-104231-fi8pskod 428 56 TGN38 TGN38 NNP cord-104231-fi8pskod 428 57 ) ) -RRB- cord-104231-fi8pskod 429 1 Golgi Golgi NNP cord-104231-fi8pskod 429 2 retention retention NN cord-104231-fi8pskod 429 3 signals signal NNS cord-104231-fi8pskod 429 4 : : : cord-104231-fi8pskod 429 5 do do VBP cord-104231-fi8pskod 429 6 membranes membrane NNS cord-104231-fi8pskod 429 7 hold hold VB cord-104231-fi8pskod 429 8 the the DT cord-104231-fi8pskod 429 9 key key NN cord-104231-fi8pskod 429 10 ? ? . cord-104231-fi8pskod 430 1 The the DT cord-104231-fi8pskod 430 2 isolation isolation NN cord-104231-fi8pskod 430 3 and and CC cord-104231-fi8pskod 430 4 nucleotide nucleotide JJ cord-104231-fi8pskod 430 5 sequence sequence NN cord-104231-fi8pskod 430 6 of of IN cord-104231-fi8pskod 430 7 a a DT cord-104231-fi8pskod 430 8 cDNA cdna NN cord-104231-fi8pskod 430 9 encoding encode VBG cord-104231-fi8pskod 430 10 the the DT cord-104231-fi8pskod 430 11 T T NNP cord-104231-fi8pskod 430 12 cell cell NN cord-104231-fi8pskod 430 13 surface surface NN cord-104231-fi8pskod 430 14 protein protein NN cord-104231-fi8pskod 430 15 T4 T4 NNP cord-104231-fi8pskod 430 16 : : : cord-104231-fi8pskod 430 17 a a DT cord-104231-fi8pskod 430 18 new new JJ cord-104231-fi8pskod 430 19 member member NN cord-104231-fi8pskod 430 20 of of IN cord-104231-fi8pskod 430 21 the the DT cord-104231-fi8pskod 430 22 immunoglobulin immunoglobulin NN cord-104231-fi8pskod 430 23 gene gene NN cord-104231-fi8pskod 430 24 family family NN cord-104231-fi8pskod 430 25 Basolateral Basolateral NNP cord-104231-fi8pskod 430 26 sorting sort VBG cord-104231-fi8pskod 430 27 of of IN cord-104231-fi8pskod 430 28 LDL ldl NN cord-104231-fi8pskod 430 29 receptor receptor NN cord-104231-fi8pskod 430 30 in in IN cord-104231-fi8pskod 430 31 MDCK MDCK NNP cord-104231-fi8pskod 430 32 cells cell NNS cord-104231-fi8pskod 431 1 : : : cord-104231-fi8pskod 431 2 the the DT cord-104231-fi8pskod 431 3 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 431 4 domain domain NN cord-104231-fi8pskod 431 5 contains contain VBZ cord-104231-fi8pskod 431 6 two two CD cord-104231-fi8pskod 431 7 tyrosinedependent tyrosinedependent NN cord-104231-fi8pskod 431 8 targeting target VBG cord-104231-fi8pskod 431 9 determinants determinant NNS cord-104231-fi8pskod 431 10 The the DT cord-104231-fi8pskod 431 11 Golgi Golgi NNP cord-104231-fi8pskod 431 12 complex complex NN cord-104231-fi8pskod 431 13 : : : cord-104231-fi8pskod 431 14 in in FW cord-104231-fi8pskod 431 15 vitro vitro FW cord-104231-fi8pskod 431 16 veritas veritas FW cord-104231-fi8pskod 431 17 ? ? . cord-104231-fi8pskod 432 1 Sequences sequence NNS cord-104231-fi8pskod 432 2 within within IN cord-104231-fi8pskod 432 3 and and CC cord-104231-fi8pskod 432 4 adjacent adjacent JJ cord-104231-fi8pskod 432 5 to to IN cord-104231-fi8pskod 432 6 the the DT cord-104231-fi8pskod 432 7 transmembrane transmembrane NN cord-104231-fi8pskod 432 8 segment segment NN cord-104231-fi8pskod 432 9 of of IN cord-104231-fi8pskod 432 10 ot-2,6-sialyltransferase ot-2,6-sialyltransferase TO cord-104231-fi8pskod 432 11 specify specify VB cord-104231-fi8pskod 432 12 Golgi Golgi NNP cord-104231-fi8pskod 432 13 retention retention NN cord-104231-fi8pskod 433 1 A a DT cord-104231-fi8pskod 433 2 C c NN cord-104231-fi8pskod 433 3 - - HYPH cord-104231-fi8pskod 433 4 terminal terminal NN cord-104231-fi8pskod 433 5 signal signal NN cord-104231-fi8pskod 433 6 prevents prevent VBZ cord-104231-fi8pskod 433 7 secretion secretion NN cord-104231-fi8pskod 433 8 of of IN cord-104231-fi8pskod 433 9 luminal luminal JJ cord-104231-fi8pskod 433 10 ER er NN cord-104231-fi8pskod 433 11 proteins protein NNS cord-104231-fi8pskod 434 1 Short short JJ cord-104231-fi8pskod 434 2 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 434 3 sequences sequence NNS cord-104231-fi8pskod 434 4 serve serve VBP cord-104231-fi8pskod 434 5 as as IN cord-104231-fi8pskod 434 6 retention retention NN cord-104231-fi8pskod 434 7 signals signal NNS cord-104231-fi8pskod 434 8 for for IN cord-104231-fi8pskod 434 9 transmembrane transmembrane NN cord-104231-fi8pskod 434 10 proteins protein NNS cord-104231-fi8pskod 434 11 in in IN cord-104231-fi8pskod 434 12 the the DT cord-104231-fi8pskod 434 13 endoplasmic endoplasmic NN cord-104231-fi8pskod 434 14 reticulum reticulum NN cord-104231-fi8pskod 435 1 The the DT cord-104231-fi8pskod 435 2 membrane membrane NN cord-104231-fi8pskod 435 3 spanning span VBG cord-104231-fi8pskod 435 4 domain domain NN cord-104231-fi8pskod 435 5 of of IN cord-104231-fi8pskod 435 6 fll,4-galactosyltransferase fll,4-galactosyltransferase NN cord-104231-fi8pskod 435 7 specifies specify VBZ cord-104231-fi8pskod 435 8 trans trans NNP cord-104231-fi8pskod 435 9 Golgi Golgi NNP cord-104231-fi8pskod 435 10 localization localization NN cord-104231-fi8pskod 435 11 Kin Kin NNP cord-104231-fi8pskod 435 12 recognition recognition NN cord-104231-fi8pskod 435 13 between between IN cord-104231-fi8pskod 435 14 medial medial JJ cord-104231-fi8pskod 435 15 Golgi Golgi NNP cord-104231-fi8pskod 435 16 enzymes enzyme NNS cord-104231-fi8pskod 435 17 in in IN cord-104231-fi8pskod 435 18 HeLa HeLa NNP cord-104231-fi8pskod 435 19 cells cell NNS cord-104231-fi8pskod 435 20 Overlapping overlap VBG cord-104231-fi8pskod 435 21 distribution distribution NN cord-104231-fi8pskod 435 22 of of IN cord-104231-fi8pskod 435 23 two two CD cord-104231-fi8pskod 435 24 glycosyltransferases glycosyltransferase NNS cord-104231-fi8pskod 435 25 in in IN cord-104231-fi8pskod 435 26 the the DT cord-104231-fi8pskod 435 27 Golgi Golgi NNP cord-104231-fi8pskod 435 28 apparatus apparatus NN cord-104231-fi8pskod 435 29 of of IN cord-104231-fi8pskod 435 30 HeLa HeLa NNP cord-104231-fi8pskod 435 31 cells cell NNS cord-104231-fi8pskod 435 32 Kin Kin NNP cord-104231-fi8pskod 435 33 recognition recognition NN cord-104231-fi8pskod 435 34 : : : cord-104231-fi8pskod 435 35 a a DT cord-104231-fi8pskod 435 36 model model NN cord-104231-fi8pskod 435 37 for for IN cord-104231-fi8pskod 435 38 the the DT cord-104231-fi8pskod 435 39 retention retention NN cord-104231-fi8pskod 435 40 of of IN cord-104231-fi8pskod 435 41 Golgi Golgi NNP cord-104231-fi8pskod 435 42 enzymes enzyme NNS cord-104231-fi8pskod 435 43 . . . cord-104231-fi8pskod 436 1 FEBS FEBS NNP cord-104231-fi8pskod 436 2 ( ( -LRB- cord-104231-fi8pskod 436 3 Fed Fed NNP cord-104231-fi8pskod 436 4 . . . cord-104231-fi8pskod 437 1 Fur Fur NNP cord-104231-fi8pskod 437 2 Perrelet Perrelet NNP cord-104231-fi8pskod 437 3 . . . cord-104231-fi8pskod 438 1 1984 1984 CD cord-104231-fi8pskod 438 2 . . . cord-104231-fi8pskod 439 1 A a DT cord-104231-fi8pskod 439 2 clathrin clathrin NN cord-104231-fi8pskod 439 3 - - HYPH cord-104231-fi8pskod 439 4 coated coat VBN cord-104231-fi8pskod 439 5 , , , cord-104231-fi8pskod 439 6 Golgi Golgi NNP cord-104231-fi8pskod 439 7 - - HYPH cord-104231-fi8pskod 439 8 related relate VBN cord-104231-fi8pskod 439 9 compartment compartment NN cord-104231-fi8pskod 439 10 of of IN cord-104231-fi8pskod 439 11 the the DT cord-104231-fi8pskod 439 12 insulin insulin NN cord-104231-fi8pskod 439 13 secreting secrete VBG cord-104231-fi8pskod 439 14 cell cell NN cord-104231-fi8pskod 439 15 accumulates accumulate VBZ cord-104231-fi8pskod 439 16 proinsulin proinsulin NNP cord-104231-fi8pskod 439 17 in in IN cord-104231-fi8pskod 439 18 the the DT cord-104231-fi8pskod 439 19 presence presence NN cord-104231-fi8pskod 439 20 of of IN cord-104231-fi8pskod 439 21 monensin monensin NNP cord-104231-fi8pskod 439 22 Clathi~-immunoreactive Clathi~-immunoreactive NNP cord-104231-fi8pskod 439 23 sites site NNS cord-104231-fi8pskod 439 24 in in IN cord-104231-fi8pskod 439 25 the the DT cord-104231-fi8pskod 439 26 Golgi Golgi NNP cord-104231-fi8pskod 439 27 apparatus apparatus NN cord-104231-fi8pskod 439 28 are be VBP cord-104231-fi8pskod 439 29 concentrated concentrate VBN cord-104231-fi8pskod 439 30 at at IN cord-104231-fi8pskod 439 31 the the DT cord-104231-fi8pskod 439 32 trans trans NNP cord-104231-fi8pskod 439 33 pole pole NN cord-104231-fi8pskod 439 34 in in IN cord-104231-fi8pskod 439 35 polypeptide polypeptide JJ cord-104231-fi8pskod 439 36 hormone hormone NN cord-104231-fi8pskod 439 37 - - HYPH cord-104231-fi8pskod 439 38 secreting secrete VBG cord-104231-fi8pskod 439 39 cells cell NNS cord-104231-fi8pskod 439 40 Clathrin Clathrin NNP cord-104231-fi8pskod 439 41 , , , cord-104231-fi8pskod 439 42 adaptors adaptor NNS cord-104231-fi8pskod 439 43 , , , cord-104231-fi8pskod 439 44 and and CC cord-104231-fi8pskod 439 45 sorting sort VBG cord-104231-fi8pskod 439 46 Differential differential JJ cord-104231-fi8pskod 439 47 endoeytosis endoeytosis NN cord-104231-fi8pskod 439 48 of of IN cord-104231-fi8pskod 439 49 CD4 CD4 NNP cord-104231-fi8pskod 439 50 in in IN cord-104231-fi8pskod 439 51 lymphoid lymphoid JJ cord-104231-fi8pskod 439 52 and and CC cord-104231-fi8pskod 439 53 non non JJ cord-104231-fi8pskod 439 54 - - JJ cord-104231-fi8pskod 439 55 lymphoid lymphoid JJ cord-104231-fi8pskod 439 56 cells cell NNS cord-104231-fi8pskod 439 57 Control Control NNP cord-104231-fi8pskod 439 58 of of IN cord-104231-fi8pskod 439 59 protein protein NN cord-104231-fi8pskod 439 60 exit exit NN cord-104231-fi8pskod 439 61 from from IN cord-104231-fi8pskod 439 62 the the DT cord-104231-fi8pskod 439 63 endoplasmic endoplasmic NN cord-104231-fi8pskod 439 64 reticulum reticulum NN cord-104231-fi8pskod 439 65 Segregation segregation NN cord-104231-fi8pskod 439 66 of of IN cord-104231-fi8pskod 439 67 MHC MHC NNP cord-104231-fi8pskod 439 68 Class Class NNP cord-104231-fi8pskod 439 69 II II NNP cord-104231-fi8pskod 439 70 molecules molecule NNS cord-104231-fi8pskod 439 71 from from IN cord-104231-fi8pskod 439 72 MHC MHC NNP cord-104231-fi8pskod 439 73 Class Class NNP cord-104231-fi8pskod 440 1 I -PRON- PRP cord-104231-fi8pskod 440 2 molecules molecule NNS cord-104231-fi8pskod 440 3 in in IN cord-104231-fi8pskod 440 4 the the DT cord-104231-fi8pskod 440 5 Golgi Golgi NNP cord-104231-fi8pskod 440 6 complex complex NN cord-104231-fi8pskod 440 7 for for IN cord-104231-fi8pskod 440 8 transport transport NN cord-104231-fi8pskod 440 9 to to IN cord-104231-fi8pskod 440 10 lysosomal lysosomal JJ cord-104231-fi8pskod 440 11 compartments compartment NNS cord-104231-fi8pskod 441 1 The the DT cord-104231-fi8pskod 441 2 differential differential JJ cord-104231-fi8pskod 441 3 degradation degradation NN cord-104231-fi8pskod 441 4 of of IN cord-104231-fi8pskod 441 5 two two CD cord-104231-fi8pskod 441 6 cytosolic cytosolic JJ cord-104231-fi8pskod 441 7 proteins protein NNS cord-104231-fi8pskod 441 8 as as IN cord-104231-fi8pskod 441 9 a a DT cord-104231-fi8pskod 441 10 tool tool NN cord-104231-fi8pskod 441 11 to to TO cord-104231-fi8pskod 441 12 monitor monitor VB cord-104231-fi8pskod 441 13 autophagy autophagy NN cord-104231-fi8pskod 441 14 in in IN cord-104231-fi8pskod 441 15 hepatocytes hepatocyte NNS cord-104231-fi8pskod 441 16 by by IN cord-104231-fi8pskod 441 17 immnnocytocbemistry immnnocytocbemistry NNP cord-104231-fi8pskod 441 18 Thrze Thrze NNP cord-104231-fi8pskod 441 19 - - HYPH cord-104231-fi8pskod 441 20 dimensional dimensional JJ cord-104231-fi8pskod 441 21 electron electron NN cord-104231-fi8pskod 441 22 microscopy microscopy NN cord-104231-fi8pskod 441 23 : : : cord-104231-fi8pskod 441 24 structure structure NN cord-104231-fi8pskod 441 25 of of IN cord-104231-fi8pskod 441 26 the the DT cord-104231-fi8pskod 441 27 Golgi Golgi NNP cord-104231-fi8pskod 441 28 apparatus apparatus NN cord-104231-fi8pskod 441 29 TGN38/41 TGN38/41 NNP cord-104231-fi8pskod 441 30 recycles recycle VBZ cord-104231-fi8pskod 441 31 between between IN cord-104231-fi8pskod 441 32 the the DT cord-104231-fi8pskod 441 33 cell cell NN cord-104231-fi8pskod 441 34 surface surface NN cord-104231-fi8pskod 441 35 and and CC cord-104231-fi8pskod 441 36 the the DT cord-104231-fi8pskod 441 37 TGN TGN NNP cord-104231-fi8pskod 441 38 : : : cord-104231-fi8pskod 442 1 brefeldin brefeldin NNP cord-104231-fi8pskod 442 2 A A NNP cord-104231-fi8pskod 442 3 affects affect VBZ cord-104231-fi8pskod 442 4 its -PRON- PRP$ cord-104231-fi8pskod 442 5 rate rate NN cord-104231-fi8pskod 442 6 of of IN cord-104231-fi8pskod 442 7 return return NN cord-104231-fi8pskod 442 8 of of IN cord-104231-fi8pskod 442 9 the the DT cord-104231-fi8pskod 442 10 TGN TGN NNP cord-104231-fi8pskod 442 11 Immunocytochemical Immunocytochemical NNP cord-104231-fi8pskod 442 12 localization localization NN cord-104231-fi8pskod 442 13 of of IN cord-104231-fi8pskod 442 14 galactosyltransferase galactosyltransferase NN cord-104231-fi8pskod 442 15 in in IN cord-104231-fi8pskod 442 16 HeLa HeLa NNP cord-104231-fi8pskod 442 17 cells cell NNS cord-104231-fi8pskod 442 18 : : : cord-104231-fi8pskod 442 19 codistribution codistribution NN cord-104231-fi8pskod 442 20 with with IN cord-104231-fi8pskod 442 21 thiamine thiamine NN cord-104231-fi8pskod 442 22 pyrophosphatase pyrophosphatase NN cord-104231-fi8pskod 442 23 in in IN cord-104231-fi8pskod 442 24 trans trans NNP cord-104231-fi8pskod 442 25 - - NNP cord-104231-fi8pskod 442 26 Golgi Golgi NNP cord-104231-fi8pskod 442 27 cisternae cisternae NNP cord-104231-fi8pskod 442 28 Demonstration Demonstration NNP cord-104231-fi8pskod 442 29 of of IN cord-104231-fi8pskod 442 30 an an DT cord-104231-fi8pskod 442 31 extensive extensive JJ cord-104231-fi8pskod 442 32 trans trans JJ cord-104231-fi8pskod 442 33 - - JJ cord-104231-fi8pskod 442 34 tubular tubular JJ cord-104231-fi8pskod 442 35 network network NN cord-104231-fi8pskod 442 36 continuous continuous JJ cord-104231-fi8pskod 442 37 with with IN cord-104231-fi8pskod 442 38 the the DT cord-104231-fi8pskod 442 39 Golgi Golgi NNP cord-104231-fi8pskod 442 40 apparatus apparatus NN cord-104231-fi8pskod 442 41 stack stack NN cord-104231-fi8pskod 442 42 that that WDT cord-104231-fi8pskod 442 43 may may MD cord-104231-fi8pskod 442 44 function function VB cord-104231-fi8pskod 442 45 in in IN cord-104231-fi8pskod 442 46 glycosylation glycosylation NN cord-104231-fi8pskod 442 47 Molecular molecular JJ cord-104231-fi8pskod 442 48 dissection dissection NN cord-104231-fi8pskod 442 49 of of IN cord-104231-fi8pskod 442 50 the the DT cord-104231-fi8pskod 442 51 secretory secretory JJ cord-104231-fi8pskod 442 52 pathway pathway NN cord-104231-fi8pskod 442 53 Primer primer NN cord-104231-fi8pskod 442 54 - - HYPH cord-104231-fi8pskod 442 55 directed direct VBN cord-104231-fi8pskod 442 56 enzymatic enzymatic JJ cord-104231-fi8pskod 442 57 amplification amplification NN cord-104231-fi8pskod 442 58 of of IN cord-104231-fi8pskod 442 59 cDNA cdna NN cord-104231-fi8pskod 442 60 with with IN cord-104231-fi8pskod 442 61 a a DT cord-104231-fi8pskod 442 62 thermostable thermostable JJ cord-104231-fi8pskod 442 63 DNA dna NN cord-104231-fi8pskod 442 64 polymerase polymerase NN cord-104231-fi8pskod 442 65 Molecular Molecular NNP cord-104231-fi8pskod 442 66 cloning clone VBG cord-104231-fi8pskod 442 67 : : : cord-104231-fi8pskod 442 68 a a DT cord-104231-fi8pskod 442 69 laboratory laboratory NN cord-104231-fi8pskod 442 70 manual manual NN cord-104231-fi8pskod 442 71 ERD2 ERD2 NNP cord-104231-fi8pskod 442 72 , , , cord-104231-fi8pskod 442 73 a a DT cord-104231-fi8pskod 442 74 yeast yeast NN cord-104231-fi8pskod 442 75 gene gene NN cord-104231-fi8pskod 442 76 required require VBN cord-104231-fi8pskod 442 77 for for IN cord-104231-fi8pskod 442 78 the the DT cord-104231-fi8pskod 442 79 receptor receptor NN cord-104231-fi8pskod 442 80 - - HYPH cord-104231-fi8pskod 442 81 mediated mediate VBN cord-104231-fi8pskod 442 82 retrieval retrieval NN cord-104231-fi8pskod 442 83 of of IN cord-104231-fi8pskod 442 84 luminal luminal JJ cord-104231-fi8pskod 442 85 ER er NN cord-104231-fi8pskod 442 86 proteins protein NNS cord-104231-fi8pskod 442 87 from from IN cord-104231-fi8pskod 442 88 the the DT cord-104231-fi8pskod 442 89 secretory secretory JJ cord-104231-fi8pskod 442 90 pathway pathway NN cord-104231-fi8pskod 442 91 Structural structural JJ cord-104231-fi8pskod 442 92 features feature NNS cord-104231-fi8pskod 442 93 of of IN cord-104231-fi8pskod 442 94 the the DT cord-104231-fi8pskod 442 95 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 442 96 region region NN cord-104231-fi8pskod 442 97 of of IN cord-104231-fi8pskod 442 98 CD4 CD4 NNP cord-104231-fi8pskod 442 99 required require VBD cord-104231-fi8pskod 442 100 for for IN cord-104231-fi8pskod 442 101 internalization internalization NN cord-104231-fi8pskod 442 102 Phosphorylationdependent Phosphorylationdependent NNP cord-104231-fi8pskod 442 103 down down IN cord-104231-fi8pskod 442 104 - - HYPH cord-104231-fi8pskod 442 105 modulation modulation NN cord-104231-fi8pskod 442 106 of of IN cord-104231-fi8pskod 442 107 CD4 CD4 NNP cord-104231-fi8pskod 442 108 requires require VBZ cord-104231-fi8pskod 442 109 a a DT cord-104231-fi8pskod 442 110 specific specific JJ cord-104231-fi8pskod 442 111 structure structure NN cord-104231-fi8pskod 442 112 within within IN cord-104231-fi8pskod 442 113 the the DT cord-104231-fi8pskod 442 114 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 442 115 domain domain NN cord-104231-fi8pskod 442 116 of of IN cord-104231-fi8pskod 442 117 CD4 CD4 NNP cord-104231-fi8pskod 442 118 The the DT cord-104231-fi8pskod 442 119 first first JJ cord-104231-fi8pskod 442 120 membrane membrane NN cord-104231-fi8pskod 442 121 spanning span VBG cord-104231-fi8pskod 442 122 region region NN cord-104231-fi8pskod 442 123 of of IN cord-104231-fi8pskod 442 124 the the DT cord-104231-fi8pskod 442 125 lamin lamin NN cord-104231-fi8pskod 442 126 B B NNP cord-104231-fi8pskod 442 127 receptor receptor NN cord-104231-fi8pskod 442 128 is be VBZ cord-104231-fi8pskod 442 129 sufficient sufficient JJ cord-104231-fi8pskod 442 130 for for IN cord-104231-fi8pskod 442 131 sorting sort VBG cord-104231-fi8pskod 442 132 to to IN cord-104231-fi8pskod 442 133 the the DT cord-104231-fi8pskod 442 134 inner inner JJ cord-104231-fi8pskod 442 135 nuclear nuclear JJ cord-104231-fi8pskod 442 136 membrane membrane NN cord-104231-fi8pskod 442 137 TGN38/41 TGN38/41 NNP cord-104231-fi8pskod 442 138 : : : cord-104231-fi8pskod 442 139 a a DT cord-104231-fi8pskod 442 140 molecule molecule NN cord-104231-fi8pskod 442 141 on on IN cord-104231-fi8pskod 442 142 the the DT cord-104231-fi8pskod 442 143 move move NN cord-104231-fi8pskod 443 1 A a DT cord-104231-fi8pskod 443 2 Golgi Golgi NNP cord-104231-fi8pskod 443 3 retention retention NN cord-104231-fi8pskod 443 4 signal signal NN cord-104231-fi8pskod 443 5 in in IN cord-104231-fi8pskod 443 6 a a DT cord-104231-fi8pskod 443 7 membrane membrane NN cord-104231-fi8pskod 443 8 spanning span VBG cord-104231-fi8pskod 443 9 domain domain NN cord-104231-fi8pskod 443 10 of of IN cord-104231-fi8pskod 443 11 coronaviros coronaviros NNP cord-104231-fi8pskod 443 12 E1 e1 NN cord-104231-fi8pskod 443 13 protein protein NN cord-104231-fi8pskod 443 14 Immunocytochemical Immunocytochemical NNP cord-104231-fi8pskod 443 15 localization localization NN cord-104231-fi8pskod 444 1 of/~1,4 of/~1,4 DT cord-104231-fi8pskod 444 2 galactosyltransferase galactosyltransferase NN cord-104231-fi8pskod 444 3 in in IN cord-104231-fi8pskod 444 4 epithelial epithelial JJ cord-104231-fi8pskod 444 5 cells cell NNS cord-104231-fi8pskod 444 6 from from IN cord-104231-fi8pskod 444 7 bovine bovine JJ cord-104231-fi8pskod 444 8 tissues tissue NNS cord-104231-fi8pskod 444 9 using use VBG cord-104231-fi8pskod 444 10 monoclonal monoclonal JJ cord-104231-fi8pskod 444 11 antibodies antibody NNS cord-104231-fi8pskod 444 12 DNA dna NN cord-104231-fi8pskod 444 13 sequence sequence NN cord-104231-fi8pskod 444 14 analysis analysis NN cord-104231-fi8pskod 444 15 with with IN cord-104231-fi8pskod 444 16 a a DT cord-104231-fi8pskod 444 17 modified modify VBN cord-104231-fi8pskod 444 18 bacteriophage bacteriophage NN cord-104231-fi8pskod 444 19 T7 t7 NN cord-104231-fi8pskod 444 20 DNA dna NN cord-104231-fi8pskod 444 21 polymerase polymerase NN cord-104231-fi8pskod 445 1 The the DT cord-104231-fi8pskod 445 2 transmembrane transmembrane NN cord-104231-fi8pskod 445 3 domain domain NN cord-104231-fi8pskod 445 4 of of IN cord-104231-fi8pskod 445 5 N n NN cord-104231-fi8pskod 445 6 - - HYPH cord-104231-fi8pskod 445 7 glucosaminyltransferase glucosaminyltransferase NN cord-104231-fi8pskod 446 1 I -PRON- PRP cord-104231-fi8pskod 446 2 contains contain VBZ cord-104231-fi8pskod 446 3 a a DT cord-104231-fi8pskod 446 4 Goigi Goigi NNP cord-104231-fi8pskod 446 5 retention retention NN cord-104231-fi8pskod 446 6 signal signal NN cord-104231-fi8pskod 446 7 . . . cord-104231-fi8pskod 447 1 3 3 LS cord-104231-fi8pskod 447 2 ' ' '' cord-104231-fi8pskod 447 3 The the DT cord-104231-fi8pskod 447 4 signal signal NN cord-104231-fi8pskod 447 5 for for IN cord-104231-fi8pskod 447 6 Golgi Golgi NNP cord-104231-fi8pskod 447 7 retention retention NN cord-104231-fi8pskod 447 8 of of IN cord-104231-fi8pskod 447 9 bovine/31,4-galactosyltransferase bovine/31,4-galactosyltransferase NN cord-104231-fi8pskod 447 10 is be VBZ cord-104231-fi8pskod 447 11 in in IN cord-104231-fi8pskod 447 12 the the DT cord-104231-fi8pskod 447 13 transmembrane transmembrane NN cord-104231-fi8pskod 447 14 doromn doromn NNP cord-104231-fi8pskod 447 15 Irmnnnochemistry Irmnnnochemistry NNP cord-104231-fi8pskod 447 16 on on IN cord-104231-fi8pskod 447 17 ultrathin ultrathin JJ cord-104231-fi8pskod 447 18 frozen frozen JJ cord-104231-fi8pskod 447 19 sections section NNS cord-104231-fi8pskod 447 20 Recycling Recycling NNP cord-104231-fi8pskod 447 21 of of IN cord-104231-fi8pskod 447 22 transferrin transferrin NN cord-104231-fi8pskod 447 23 receptors receptor NNS cord-104231-fi8pskod 447 24 in in IN cord-104231-fi8pskod 447 25 A431 A431 NNP cord-104231-fi8pskod 447 26 cells cell NNS cord-104231-fi8pskod 447 27 is be VBZ cord-104231-fi8pskod 447 28 inhibited inhibit VBN cord-104231-fi8pskod 447 29 during during IN cord-104231-fi8pskod 447 30 mitosis mitosis NN cord-104231-fi8pskod 448 1 The the DT cord-104231-fi8pskod 448 2 SXYQRL SXYQRL NNP cord-104231-fi8pskod 448 3 sequence sequence NN cord-104231-fi8pskod 448 4 in in IN cord-104231-fi8pskod 448 5 the the DT cord-104231-fi8pskod 448 6 cytoplasmic cytoplasmic JJ cord-104231-fi8pskod 448 7 domain domain NN cord-104231-fi8pskod 448 8 of of IN cord-104231-fi8pskod 448 9 TGN38 TGN38 NNP cord-104231-fi8pskod 448 10 plays play VBZ cord-104231-fi8pskod 448 11 a a DT cord-104231-fi8pskod 448 12 major major JJ cord-104231-fi8pskod 448 13 role role NN cord-104231-fi8pskod 448 14 in in IN cord-104231-fi8pskod 448 15 trans trans NNP cord-104231-fi8pskod 448 16 - - NNP cord-104231-fi8pskod 448 17 Golgi Golgi NNP cord-104231-fi8pskod 448 18 network network NN cord-104231-fi8pskod 448 19 localization localization NN cord-104231-fi8pskod 449 1 The the DT cord-104231-fi8pskod 449 2 17-residue 17-residue CD cord-104231-fi8pskod 449 3 membrane membrane NN cord-104231-fi8pskod 449 4 domain domain NN cord-104231-fi8pskod 450 1 of/~-galactoside of/~-galactoside JJ cord-104231-fi8pskod 450 2 tx2,6-sialyltransferase tx2,6-sialyltransferase NNP cord-104231-fi8pskod 450 3 is be VBZ cord-104231-fi8pskod 450 4 sufficient sufficient JJ cord-104231-fi8pskod 450 5 for for IN cord-104231-fi8pskod 450 6 Golgi Golgi NNP cord-104231-fi8pskod 450 7 retention retention NN cord-104231-fi8pskod 451 1 The the DT cord-104231-fi8pskod 451 2 single single JJ cord-104231-fi8pskod 451 3 transmembrane transmembrane NN cord-104231-fi8pskod 451 4 segment segment NN cord-104231-fi8pskod 451 5 of of IN cord-104231-fi8pskod 451 6 gp210 gp210 NNP cord-104231-fi8pskod 451 7 is be VBZ cord-104231-fi8pskod 451 8 sufficient sufficient JJ cord-104231-fi8pskod 451 9 for for IN cord-104231-fi8pskod 451 10 sorting sort VBG cord-104231-fi8pskod 451 11 to to IN cord-104231-fi8pskod 451 12 the the DT cord-104231-fi8pskod 451 13 pore pore NN cord-104231-fi8pskod 451 14 membrane membrane NN cord-104231-fi8pskod 451 15 domain domain NN cord-104231-fi8pskod 451 16 of of IN cord-104231-fi8pskod 451 17 the the DT cord-104231-fi8pskod 451 18 nuclear nuclear JJ cord-104231-fi8pskod 451 19 envelope envelope NN cord-104231-fi8pskod 452 1 We -PRON- PRP cord-104231-fi8pskod 452 2 thank thank VBP cord-104231-fi8pskod 452 3 Dr. Dr. NNP cord-104231-fi8pskod 452 4 Mark Mark NNP cord-104231-fi8pskod 452 5 Marsh Marsh NNP cord-104231-fi8pskod 452 6 for for IN cord-104231-fi8pskod 452 7 helpful helpful JJ cord-104231-fi8pskod 452 8 advice advice NN cord-104231-fi8pskod 452 9 and and CC cord-104231-fi8pskod 452 10 antibodies antibody NNS cord-104231-fi8pskod 452 11 , , , cord-104231-fi8pskod 452 12 Dr. Dr. NNP cord-104231-fi8pskod 452 13 Eric Eric NNP cord-104231-fi8pskod 452 14 Berger Berger NNP cord-104231-fi8pskod 452 15 , , , cord-104231-fi8pskod 452 16 Dr. Dr. NNP cord-104231-fi8pskod 452 17 Peter Peter NNP cord-104231-fi8pskod 452 18 Beverly Beverly NNP cord-104231-fi8pskod 452 19 , , , cord-104231-fi8pskod 452 20 and and CC cord-104231-fi8pskod 452 21 Dr Dr NNP cord-104231-fi8pskod 452 22 , , , cord-104231-fi8pskod 452 23 Nancy Nancy NNP cord-104231-fi8pskod 452 24 Hogg Hogg NNP cord-104231-fi8pskod 452 25 for for IN cord-104231-fi8pskod 452 26 antibodies antibody NNS cord-104231-fi8pskod 452 27 , , , cord-104231-fi8pskod 452 28 and and CC cord-104231-fi8pskod 452 29 Dr. Dr. NNP cord-104231-fi8pskod 452 30 Per Per NNP cord-104231-fi8pskod 452 31 Peterson Peterson NNP cord-104231-fi8pskod 452 32 for for IN cord-104231-fi8pskod 452 33 the the DT cord-104231-fi8pskod 452 34 CD4 CD4 NNP cord-104231-fi8pskod 452 35 cDNA cdna NN cord-104231-fi8pskod 452 36 . . . cord-104231-fi8pskod 453 1 We -PRON- PRP cord-104231-fi8pskod 453 2 also also RB cord-104231-fi8pskod 453 3 thank thank VBP cord-104231-fi8pskod 453 4 Rose Rose NNP cord-104231-fi8pskod 453 5 Watson Watson NNP cord-104231-fi8pskod 453 6 , , , cord-104231-fi8pskod 453 7 Felicia Felicia NNP cord-104231-fi8pskod 453 8 Hunte Hunte NNP cord-104231-fi8pskod 453 9 , , , cord-104231-fi8pskod 453 10 and and CC cord-104231-fi8pskod 453 11 the the DT cord-104231-fi8pskod 453 12 Imperial Imperial NNP cord-104231-fi8pskod 453 13 Cancer Cancer NNP cord-104231-fi8pskod 453 14 Research Research NNP cord-104231-fi8pskod 453 15 Fund Fund NNP cord-104231-fi8pskod 453 16 ( ( -LRB- cord-104231-fi8pskod 453 17 ICRF ICRF NNP cord-104231-fi8pskod 453 18 ) ) -RRB- cord-104231-fi8pskod 453 19 FACS FACS NNP cord-104231-fi8pskod 453 20 Unit Unit NNP cord-104231-fi8pskod 453 21 for for IN cord-104231-fi8pskod 453 22 invaluable invaluable JJ cord-104231-fi8pskod 453 23 technical technical JJ cord-104231-fi8pskod 453 24 assistance assistance NN cord-104231-fi8pskod 453 25 . . . cord-104231-fi8pskod 454 1 We -PRON- PRP cord-104231-fi8pskod 454 2 thank thank VBP cord-104231-fi8pskod 454 3 the the DT cord-104231-fi8pskod 454 4 ICRF ICRF NNP cord-104231-fi8pskod 454 5 Oligonucleotide Oligonucleotide NNP cord-104231-fi8pskod 454 6 Synthesis Synthesis NNP cord-104231-fi8pskod 454 7 Facility Facility NNP cord-104231-fi8pskod 454 8 for for IN cord-104231-fi8pskod 454 9 high high JJ cord-104231-fi8pskod 454 10 quality quality NN cord-104231-fi8pskod 454 11 oligonuclootides oligonuclootide NNS cord-104231-fi8pskod 454 12 and and CC cord-104231-fi8pskod 454 13 the the DT cord-104231-fi8pskod 454 14 Photography Photography NNP cord-104231-fi8pskod 454 15 Department Department NNP cord-104231-fi8pskod 454 16 for for IN cord-104231-fi8pskod 454 17 their -PRON- PRP$ cord-104231-fi8pskod 454 18 help help NN cord-104231-fi8pskod 454 19 . . . cord-104231-fi8pskod 455 1 We -PRON- PRP cord-104231-fi8pskod 455 2 thank thank VBP cord-104231-fi8pskod 455 3 Dr. Dr. NNP cord-104231-fi8pskod 455 4 Kathryn Kathryn NNP cord-104231-fi8pskod 455 5 Howell Howell NNP cord-104231-fi8pskod 455 6 , , , cord-104231-fi8pskod 455 7 Dr. Dr. NNP cord-104231-fi8pskod 455 8 Wanjin Wanjin NNP cord-104231-fi8pskod 455 9 Hong Hong NNP cord-104231-fi8pskod 455 10 , , , cord-104231-fi8pskod 455 11 and and CC cord-104231-fi8pskod 455 12 Dr. Dr. NNP cord-104231-fi8pskod 455 13 Keith Keith NNP cord-104231-fi8pskod 455 14 Stanley Stanley NNP cord-104231-fi8pskod 455 15 for for IN cord-104231-fi8pskod 455 16 preprints preprint NNS cord-104231-fi8pskod 455 17 of of IN cord-104231-fi8pskod 455 18 papers paper NNS cord-104231-fi8pskod 455 19 before before IN cord-104231-fi8pskod 455 20 publication publication NN cord-104231-fi8pskod 455 21 and and CC cord-104231-fi8pskod 455 22 helpful helpful JJ cord-104231-fi8pskod 455 23 advice advice NN cord-104231-fi8pskod 455 24 . . . cord-104231-fi8pskod 456 1 We -PRON- PRP cord-104231-fi8pskod 456 2 also also RB cord-104231-fi8pskod 456 3 thank thank VBP cord-104231-fi8pskod 456 4 members member NNS cord-104231-fi8pskod 456 5 of of IN cord-104231-fi8pskod 456 6 the the DT cord-104231-fi8pskod 456 7 Warren Warren NNP cord-104231-fi8pskod 456 8 lab lab NN cord-104231-fi8pskod 456 9 for for IN cord-104231-fi8pskod 456 10 advice advice NN cord-104231-fi8pskod 456 11 and and CC cord-104231-fi8pskod 456 12 critical critical JJ cord-104231-fi8pskod 456 13 reading reading NN cord-104231-fi8pskod 456 14 of of IN cord-104231-fi8pskod 456 15 the the DT cord-104231-fi8pskod 456 16 manuscript manuscript NN cord-104231-fi8pskod 456 17 . . . cord-104231-fi8pskod 457 1 Received receive VBN cord-104231-fi8pskod 457 2 for for IN cord-104231-fi8pskod 457 3 publication publication NN cord-104231-fi8pskod 457 4 4 4 CD cord-104231-fi8pskod 457 5 October October NNP cord-104231-fi8pskod 457 6 1993 1993 CD cord-104231-fi8pskod 457 7 and and CC cord-104231-fi8pskod 457 8 in in IN cord-104231-fi8pskod 457 9 revised revise VBN cord-104231-fi8pskod 457 10 form form NN cord-104231-fi8pskod 457 11 7 7 CD cord-104231-fi8pskod 457 12 December December NNP cord-104231-fi8pskod 457 13 1993 1993 CD cord-104231-fi8pskod 457 14 . . .