id sid tid token lemma pos cord-104269-9r7rqqqk 1 1 key key JJ cord-104269-9r7rqqqk 2 1 : : : cord-104269-9r7rqqqk 2 2 cord-104269 cord-104269 NNP cord-104269-9r7rqqqk 2 3 - - HYPH cord-104269-9r7rqqqk 2 4 9r7rqqqk 9r7rqqqk CD cord-104269-9r7rqqqk 2 5 authors author NNS cord-104269-9r7rqqqk 2 6 : : : cord-104269-9r7rqqqk 3 1 nan nan NNP cord-104269-9r7rqqqk 3 2 title title NN cord-104269-9r7rqqqk 3 3 : : : cord-104269-9r7rqqqk 4 1 Sorting sorting NN cord-104269-9r7rqqqk 4 2 signals signal NNS cord-104269-9r7rqqqk 4 3 in in IN cord-104269-9r7rqqqk 4 4 the the DT cord-104269-9r7rqqqk 4 5 MHC MHC NNP cord-104269-9r7rqqqk 4 6 class class NN cord-104269-9r7rqqqk 4 7 II II NNP cord-104269-9r7rqqqk 4 8 invariant invariant JJ cord-104269-9r7rqqqk 4 9 chain chain NN cord-104269-9r7rqqqk 4 10 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 4 11 tail tail NN cord-104269-9r7rqqqk 4 12 and and CC cord-104269-9r7rqqqk 4 13 transmembrane transmembrane NN cord-104269-9r7rqqqk 4 14 region region NN cord-104269-9r7rqqqk 4 15 determine determine VBP cord-104269-9r7rqqqk 4 16 trafficking traffic VBG cord-104269-9r7rqqqk 4 17 to to IN cord-104269-9r7rqqqk 4 18 an an DT cord-104269-9r7rqqqk 4 19 endocytic endocytic JJ cord-104269-9r7rqqqk 4 20 processing processing NN cord-104269-9r7rqqqk 4 21 compartment compartment NN cord-104269-9r7rqqqk 4 22 date date NN cord-104269-9r7rqqqk 4 23 : : : cord-104269-9r7rqqqk 4 24 1994 1994 CD cord-104269-9r7rqqqk 4 25 - - SYM cord-104269-9r7rqqqk 4 26 07 07 CD cord-104269-9r7rqqqk 4 27 - - HYPH cord-104269-9r7rqqqk 4 28 02 02 CD cord-104269-9r7rqqqk 4 29 journal journal NN cord-104269-9r7rqqqk 4 30 : : : cord-104269-9r7rqqqk 5 1 J J NNP cord-104269-9r7rqqqk 5 2 Cell Cell NNP cord-104269-9r7rqqqk 5 3 Biol Biol NNP cord-104269-9r7rqqqk 5 4 DOI DOI NNP cord-104269-9r7rqqqk 5 5 : : : cord-104269-9r7rqqqk 5 6 nan nan NNP cord-104269-9r7rqqqk 5 7 sha sha NNP cord-104269-9r7rqqqk 5 8 : : : cord-104269-9r7rqqqk 6 1 6220d2580a3a4a5c86ea58944be947b5d4c0bc0b 6220d2580a3a4a5c86ea58944be947b5d4c0bc0b CD cord-104269-9r7rqqqk 6 2 doc_id doc_id CD cord-104269-9r7rqqqk 7 1 : : : cord-104269-9r7rqqqk 7 2 104269 104269 CD cord-104269-9r7rqqqk 7 3 cord_uid cord_uid NNS cord-104269-9r7rqqqk 7 4 : : : cord-104269-9r7rqqqk 7 5 9r7rqqqk 9r7rqqqk CD cord-104269-9r7rqqqk 8 1 Targeting target VBG cord-104269-9r7rqqqk 8 2 of of IN cord-104269-9r7rqqqk 8 3 MHC MHC NNP cord-104269-9r7rqqqk 8 4 class class NN cord-104269-9r7rqqqk 8 5 II II NNP cord-104269-9r7rqqqk 8 6 molecules molecule NNS cord-104269-9r7rqqqk 8 7 to to IN cord-104269-9r7rqqqk 8 8 the the DT cord-104269-9r7rqqqk 8 9 endocytic endocytic JJ cord-104269-9r7rqqqk 8 10 compartment compartment NN cord-104269-9r7rqqqk 8 11 where where WRB cord-104269-9r7rqqqk 8 12 they -PRON- PRP cord-104269-9r7rqqqk 8 13 encounter encounter VBP cord-104269-9r7rqqqk 8 14 processed process VBN cord-104269-9r7rqqqk 8 15 antigen antigen NN cord-104269-9r7rqqqk 8 16 is be VBZ cord-104269-9r7rqqqk 8 17 determined determine VBN cord-104269-9r7rqqqk 8 18 by by IN cord-104269-9r7rqqqk 8 19 the the DT cord-104269-9r7rqqqk 8 20 invariant invariant JJ cord-104269-9r7rqqqk 8 21 chain chain NN cord-104269-9r7rqqqk 8 22 ( ( -LRB- cord-104269-9r7rqqqk 8 23 Ii Ii NNP cord-104269-9r7rqqqk 8 24 ) ) -RRB- cord-104269-9r7rqqqk 8 25 . . . cord-104269-9r7rqqqk 9 1 By by IN cord-104269-9r7rqqqk 9 2 analysis analysis NN cord-104269-9r7rqqqk 9 3 of of IN cord-104269-9r7rqqqk 9 4 Ii ii NN cord-104269-9r7rqqqk 9 5 - - HYPH cord-104269-9r7rqqqk 9 6 transferrin transferrin NN cord-104269-9r7rqqqk 9 7 receptor receptor NN cord-104269-9r7rqqqk 9 8 ( ( -LRB- cord-104269-9r7rqqqk 9 9 TR tr NN cord-104269-9r7rqqqk 9 10 ) ) -RRB- cord-104269-9r7rqqqk 9 11 chimera chimera NN cord-104269-9r7rqqqk 9 12 trafficking trafficking NN cord-104269-9r7rqqqk 9 13 , , , cord-104269-9r7rqqqk 9 14 we -PRON- PRP cord-104269-9r7rqqqk 9 15 have have VBP cord-104269-9r7rqqqk 9 16 identified identify VBN cord-104269-9r7rqqqk 9 17 sorting sorting NN cord-104269-9r7rqqqk 9 18 signals signal NNS cord-104269-9r7rqqqk 9 19 in in IN cord-104269-9r7rqqqk 9 20 the the DT cord-104269-9r7rqqqk 9 21 Ii Ii NNP cord-104269-9r7rqqqk 9 22 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 9 23 tail tail NN cord-104269-9r7rqqqk 9 24 and and CC cord-104269-9r7rqqqk 9 25 transmembrane transmembrane NN cord-104269-9r7rqqqk 9 26 region region NN cord-104269-9r7rqqqk 9 27 that that WDT cord-104269-9r7rqqqk 9 28 mediate mediate VBP cord-104269-9r7rqqqk 9 29 this this DT cord-104269-9r7rqqqk 9 30 process process NN cord-104269-9r7rqqqk 9 31 . . . cord-104269-9r7rqqqk 10 1 Two two CD cord-104269-9r7rqqqk 10 2 non non JJ cord-104269-9r7rqqqk 10 3 - - JJ cord-104269-9r7rqqqk 10 4 tyrosine tyrosine NN cord-104269-9r7rqqqk 10 5 - - HYPH cord-104269-9r7rqqqk 10 6 based base VBN cord-104269-9r7rqqqk 10 7 sorting sorting NN cord-104269-9r7rqqqk 10 8 signals signal NNS cord-104269-9r7rqqqk 10 9 in in IN cord-104269-9r7rqqqk 10 10 the the DT cord-104269-9r7rqqqk 10 11 Ii Ii NNP cord-104269-9r7rqqqk 10 12 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 10 13 tail tail NN cord-104269-9r7rqqqk 10 14 were be VBD cord-104269-9r7rqqqk 10 15 identified identify VBN cord-104269-9r7rqqqk 10 16 that that IN cord-104269-9r7rqqqk 10 17 mediate mediate VBP cord-104269-9r7rqqqk 10 18 localization localization NN cord-104269-9r7rqqqk 10 19 to to IN cord-104269-9r7rqqqk 10 20 plasma plasma NN cord-104269-9r7rqqqk 10 21 membrane membrane NN cord-104269-9r7rqqqk 10 22 clathrin clathrin NN cord-104269-9r7rqqqk 10 23 - - HYPH cord-104269-9r7rqqqk 10 24 coated coat VBN cord-104269-9r7rqqqk 10 25 pits pit NNS cord-104269-9r7rqqqk 10 26 and and CC cord-104269-9r7rqqqk 10 27 promote promote VB cord-104269-9r7rqqqk 10 28 rapid rapid JJ cord-104269-9r7rqqqk 10 29 endocytosis endocytosis NN cord-104269-9r7rqqqk 10 30 . . . cord-104269-9r7rqqqk 11 1 Leu7 Leu7 NNP cord-104269-9r7rqqqk 11 2 and and CC cord-104269-9r7rqqqk 11 3 Ile8 ile8 PRP cord-104269-9r7rqqqk 11 4 were be VBD cord-104269-9r7rqqqk 11 5 required require VBN cord-104269-9r7rqqqk 11 6 for for IN cord-104269-9r7rqqqk 11 7 the the DT cord-104269-9r7rqqqk 11 8 activity activity NN cord-104269-9r7rqqqk 11 9 of of IN cord-104269-9r7rqqqk 11 10 the the DT cord-104269-9r7rqqqk 11 11 signal signal NN cord-104269-9r7rqqqk 11 12 most most RBS cord-104269-9r7rqqqk 11 13 distal distal JJ cord-104269-9r7rqqqk 11 14 to to IN cord-104269-9r7rqqqk 11 15 the the DT cord-104269-9r7rqqqk 11 16 cell cell NN cord-104269-9r7rqqqk 11 17 membrane membrane NN cord-104269-9r7rqqqk 11 18 whereas whereas IN cord-104269-9r7rqqqk 11 19 Pro15 Pro15 NNP cord-104269-9r7rqqqk 11 20 Met16 Met16 NNP cord-104269-9r7rqqqk 11 21 Leu17 Leu17 NNP cord-104269-9r7rqqqk 11 22 were be VBD cord-104269-9r7rqqqk 11 23 important important JJ cord-104269-9r7rqqqk 11 24 for for IN cord-104269-9r7rqqqk 11 25 the the DT cord-104269-9r7rqqqk 11 26 membrane membrane NN cord-104269-9r7rqqqk 11 27 - - HYPH cord-104269-9r7rqqqk 11 28 proximal proximal JJ cord-104269-9r7rqqqk 11 29 signal signal NN cord-104269-9r7rqqqk 11 30 . . . cord-104269-9r7rqqqk 12 1 The the DT cord-104269-9r7rqqqk 12 2 same same JJ cord-104269-9r7rqqqk 12 3 or or CC cord-104269-9r7rqqqk 12 4 overlapping overlap VBG cord-104269-9r7rqqqk 12 5 non- non- DT cord-104269-9r7rqqqk 12 6 tyrosine tyrosine NN cord-104269-9r7rqqqk 12 7 - - HYPH cord-104269-9r7rqqqk 12 8 based base VBN cord-104269-9r7rqqqk 12 9 sorting sorting NN cord-104269-9r7rqqqk 12 10 signals signal NNS cord-104269-9r7rqqqk 12 11 are be VBP cord-104269-9r7rqqqk 12 12 essential essential JJ cord-104269-9r7rqqqk 12 13 for for IN cord-104269-9r7rqqqk 12 14 delivery delivery NN cord-104269-9r7rqqqk 12 15 of of IN cord-104269-9r7rqqqk 12 16 Ii Ii NNP cord-104269-9r7rqqqk 12 17 - - HYPH cord-104269-9r7rqqqk 12 18 TR TR NNP cord-104269-9r7rqqqk 12 19 chimeras chimera NNS cord-104269-9r7rqqqk 12 20 , , , cord-104269-9r7rqqqk 12 21 either either CC cord-104269-9r7rqqqk 12 22 by by IN cord-104269-9r7rqqqk 12 23 an an DT cord-104269-9r7rqqqk 12 24 intracellular intracellular JJ cord-104269-9r7rqqqk 12 25 route route NN cord-104269-9r7rqqqk 12 26 or or CC cord-104269-9r7rqqqk 12 27 via via IN cord-104269-9r7rqqqk 12 28 the the DT cord-104269-9r7rqqqk 12 29 plasma plasma NN cord-104269-9r7rqqqk 12 30 membrane membrane NN cord-104269-9r7rqqqk 12 31 , , , cord-104269-9r7rqqqk 12 32 to to IN cord-104269-9r7rqqqk 12 33 an an DT cord-104269-9r7rqqqk 12 34 endocytic endocytic JJ cord-104269-9r7rqqqk 12 35 compartment compartment NN cord-104269-9r7rqqqk 12 36 where where WRB cord-104269-9r7rqqqk 12 37 they -PRON- PRP cord-104269-9r7rqqqk 12 38 are be VBP cord-104269-9r7rqqqk 12 39 rapidly rapidly RB cord-104269-9r7rqqqk 12 40 degraded degrade VBN cord-104269-9r7rqqqk 12 41 . . . cord-104269-9r7rqqqk 13 1 The the DT cord-104269-9r7rqqqk 13 2 Ii Ii NNP cord-104269-9r7rqqqk 13 3 transmembrane transmembrane NNP cord-104269-9r7rqqqk 13 4 region region NN cord-104269-9r7rqqqk 13 5 is be VBZ cord-104269-9r7rqqqk 13 6 also also RB cord-104269-9r7rqqqk 13 7 required require VBN cord-104269-9r7rqqqk 13 8 for for IN cord-104269-9r7rqqqk 13 9 efficient efficient JJ cord-104269-9r7rqqqk 13 10 delivery delivery NN cord-104269-9r7rqqqk 13 11 to to IN cord-104269-9r7rqqqk 13 12 this this DT cord-104269-9r7rqqqk 13 13 endocytic endocytic JJ cord-104269-9r7rqqqk 13 14 processing processing NN cord-104269-9r7rqqqk 13 15 compartment compartment NN cord-104269-9r7rqqqk 13 16 and and CC cord-104269-9r7rqqqk 13 17 contains contain VBZ cord-104269-9r7rqqqk 13 18 a a DT cord-104269-9r7rqqqk 13 19 signal signal NN cord-104269-9r7rqqqk 13 20 distinct distinct JJ cord-104269-9r7rqqqk 13 21 from from IN cord-104269-9r7rqqqk 13 22 the the DT cord-104269-9r7rqqqk 13 23 Ii Ii NNP cord-104269-9r7rqqqk 13 24 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 13 25 tail tail NN cord-104269-9r7rqqqk 13 26 . . . cord-104269-9r7rqqqk 14 1 More More JJR cord-104269-9r7rqqqk 14 2 than than IN cord-104269-9r7rqqqk 14 3 80 80 CD cord-104269-9r7rqqqk 14 4 % % NN cord-104269-9r7rqqqk 14 5 of of IN cord-104269-9r7rqqqk 14 6 the the DT cord-104269-9r7rqqqk 14 7 Ii Ii NNP cord-104269-9r7rqqqk 14 8 - - HYPH cord-104269-9r7rqqqk 14 9 TR TR NNP cord-104269-9r7rqqqk 14 10 chimera chimera NN cord-104269-9r7rqqqk 14 11 containing contain VBG cord-104269-9r7rqqqk 14 12 the the DT cord-104269-9r7rqqqk 14 13 Ii Ii NNP cord-104269-9r7rqqqk 14 14 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 14 15 tail tail NN cord-104269-9r7rqqqk 14 16 and and CC cord-104269-9r7rqqqk 14 17 transmembrane transmembrane NN cord-104269-9r7rqqqk 14 18 region region NN cord-104269-9r7rqqqk 14 19 is be VBZ cord-104269-9r7rqqqk 14 20 delivered deliver VBN cord-104269-9r7rqqqk 14 21 directly directly RB cord-104269-9r7rqqqk 14 22 to to IN cord-104269-9r7rqqqk 14 23 the the DT cord-104269-9r7rqqqk 14 24 endocytic endocytic JJ cord-104269-9r7rqqqk 14 25 pathway pathway NN cord-104269-9r7rqqqk 14 26 by by IN cord-104269-9r7rqqqk 14 27 an an DT cord-104269-9r7rqqqk 14 28 intracellular intracellular JJ cord-104269-9r7rqqqk 14 29 route route NN cord-104269-9r7rqqqk 14 30 , , , cord-104269-9r7rqqqk 14 31 implying imply VBG cord-104269-9r7rqqqk 14 32 that that IN cord-104269-9r7rqqqk 14 33 the the DT cord-104269-9r7rqqqk 14 34 Ii Ii NNP cord-104269-9r7rqqqk 14 35 sorting sorting NN cord-104269-9r7rqqqk 14 36 signals signal NNS cord-104269-9r7rqqqk 14 37 are be VBP cord-104269-9r7rqqqk 14 38 efficiently efficiently RB cord-104269-9r7rqqqk 14 39 recognized recognize VBN cord-104269-9r7rqqqk 14 40 by by IN cord-104269-9r7rqqqk 14 41 sorting sort VBG cord-104269-9r7rqqqk 14 42 machinery machinery NN cord-104269-9r7rqqqk 14 43 located locate VBN cord-104269-9r7rqqqk 14 44 in in IN cord-104269-9r7rqqqk 14 45 the the DT cord-104269-9r7rqqqk 14 46 trans trans NNP cord-104269-9r7rqqqk 14 47 - - NNP cord-104269-9r7rqqqk 14 48 Golgi Golgi NNP cord-104269-9r7rqqqk 14 49 . . . cord-104269-9r7rqqqk 15 1 Ii Ii NNP cord-104269-9r7rqqqk 15 2 - - HYPH cord-104269-9r7rqqqk 15 3 TR TR NNP cord-104269-9r7rqqqk 15 4 chimera chimera NN cord-104269-9r7rqqqk 15 5 containing contain VBG cord-104269-9r7rqqqk 15 6 the the DT cord-104269-9r7rqqqk 15 7 Ii Ii NNP cord-104269-9r7rqqqk 15 8 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 15 9 tail tail NN cord-104269-9r7rqqqk 15 10 and and CC cord-104269-9r7rqqqk 15 11 transmembrane transmembrane NN cord-104269-9r7rqqqk 15 12 region region NN cord-104269-9r7rqqqk 15 13 is be VBZ cord-104269-9r7rqqqk 15 14 delivered deliver VBN cord-104269-9r7rqqqk 15 15 directly directly RB cord-104269-9r7rqqqk 15 16 to to IN cord-104269-9r7rqqqk 15 17 the the DT cord-104269-9r7rqqqk 15 18 endocytic endocytic JJ cord-104269-9r7rqqqk 15 19 pathway pathway NN cord-104269-9r7rqqqk 15 20 by by IN cord-104269-9r7rqqqk 15 21 an an DT cord-104269-9r7rqqqk 15 22 intracellular intracellular JJ cord-104269-9r7rqqqk 15 23 route route NN cord-104269-9r7rqqqk 15 24 , , , cord-104269-9r7rqqqk 15 25 implying imply VBG cord-104269-9r7rqqqk 15 26 that that IN cord-104269-9r7rqqqk 15 27 the the DT cord-104269-9r7rqqqk 15 28 Ii Ii NNP cord-104269-9r7rqqqk 15 29 sorting sorting NN cord-104269-9r7rqqqk 15 30 signals signal NNS cord-104269-9r7rqqqk 15 31 are be VBP cord-104269-9r7rqqqk 15 32 efficiently efficiently RB cord-104269-9r7rqqqk 15 33 recognized recognize VBN cord-104269-9r7rqqqk 15 34 by by IN cord-104269-9r7rqqqk 15 35 sorting sort VBG cord-104269-9r7rqqqk 15 36 machinery machinery NN cord-104269-9r7rqqqk 15 37 located locate VBN cord-104269-9r7rqqqk 15 38 in in IN cord-104269-9r7rqqqk 15 39 the the DT cord-104269-9r7rqqqk 15 40 trans trans NNP cord-104269-9r7rqqqk 15 41 - - NNP cord-104269-9r7rqqqk 15 42 Golgi Golgi NNP cord-104269-9r7rqqqk 15 43 . . . cord-104269-9r7rqqqk 16 1 M M NNP cord-104269-9r7rqqqk 16 2 AJOR AJOR NNP cord-104269-9r7rqqqk 16 3 histocompatibility histocompatibility NN cord-104269-9r7rqqqk 16 4 complex complex NN cord-104269-9r7rqqqk 16 5 ( ( -LRB- cord-104269-9r7rqqqk 17 1 MHC MHC NNP cord-104269-9r7rqqqk 17 2 ) ) -RRB- cord-104269-9r7rqqqk 17 3 1 1 CD cord-104269-9r7rqqqk 17 4 class class NN cord-104269-9r7rqqqk 17 5 1I 1i CD cord-104269-9r7rqqqk 17 6 molecules molecule NNS cord-104269-9r7rqqqk 17 7 are be VBP cord-104269-9r7rqqqk 17 8 polymorphic polymorphic JJ cord-104269-9r7rqqqk 17 9 cell cell NN cord-104269-9r7rqqqk 17 10 surface surface NN cord-104269-9r7rqqqk 17 11 glycoproteins glycoprotein NNS cord-104269-9r7rqqqk 17 12 expressed express VBD cord-104269-9r7rqqqk 17 13 primarily primarily RB cord-104269-9r7rqqqk 17 14 on on IN cord-104269-9r7rqqqk 17 15 specialized specialized JJ cord-104269-9r7rqqqk 17 16 antigenpresenting antigenpresenting NN cord-104269-9r7rqqqk 17 17 cells cell NNS cord-104269-9r7rqqqk 17 18 such such JJ cord-104269-9r7rqqqk 17 19 as as IN cord-104269-9r7rqqqk 17 20 macrophages macrophage NNS cord-104269-9r7rqqqk 17 21 , , , cord-104269-9r7rqqqk 17 22 dendritic dendritic JJ cord-104269-9r7rqqqk 17 23 cells cell NNS cord-104269-9r7rqqqk 17 24 , , , cord-104269-9r7rqqqk 17 25 and and CC cord-104269-9r7rqqqk 17 26 B b NN cord-104269-9r7rqqqk 17 27 lymphocytes lymphocyte NNS cord-104269-9r7rqqqk 17 28 . . . cord-104269-9r7rqqqk 18 1 They -PRON- PRP cord-104269-9r7rqqqk 18 2 bind bind VBP cord-104269-9r7rqqqk 18 3 peptides peptide NNS cord-104269-9r7rqqqk 18 4 derived derive VBN cord-104269-9r7rqqqk 18 5 from from IN cord-104269-9r7rqqqk 18 6 exogenous exogenous JJ cord-104269-9r7rqqqk 18 7 proteins protein NNS cord-104269-9r7rqqqk 18 8 and and CC cord-104269-9r7rqqqk 18 9 present present VB cord-104269-9r7rqqqk 18 10 them -PRON- PRP cord-104269-9r7rqqqk 18 11 to to IN cord-104269-9r7rqqqk 18 12 CD4 CD4 NNP cord-104269-9r7rqqqk 18 13 + + CC cord-104269-9r7rqqqk 18 14 helper helper NN cord-104269-9r7rqqqk 18 15 T t NN cord-104269-9r7rqqqk 18 16 cells cell NNS cord-104269-9r7rqqqk 18 17 as as IN cord-104269-9r7rqqqk 18 18 part part NN cord-104269-9r7rqqqk 18 19 of of IN cord-104269-9r7rqqqk 18 20 the the DT cord-104269-9r7rqqqk 18 21 mechanism mechanism NN cord-104269-9r7rqqqk 18 22 for for IN cord-104269-9r7rqqqk 18 23 recognizing recognize VBG cord-104269-9r7rqqqk 18 24 foreign foreign JJ cord-104269-9r7rqqqk 18 25 antigens antigen NNS cord-104269-9r7rqqqk 18 26 and and CC cord-104269-9r7rqqqk 18 27 stimulating stimulate VBG cord-104269-9r7rqqqk 18 28 an an DT cord-104269-9r7rqqqk 18 29 immune immune JJ cord-104269-9r7rqqqk 18 30 response response NN cord-104269-9r7rqqqk 18 31 . . . cord-104269-9r7rqqqk 19 1 MHC MHC NNP cord-104269-9r7rqqqk 19 2 class class NN cord-104269-9r7rqqqk 19 3 II II NNP cord-104269-9r7rqqqk 19 4 molecules molecule NNS cord-104269-9r7rqqqk 19 5 displayed display VBN cord-104269-9r7rqqqk 19 6 on on IN cord-104269-9r7rqqqk 19 7 the the DT cord-104269-9r7rqqqk 19 8 cell cell NN cord-104269-9r7rqqqk 19 9 surface surface NN cord-104269-9r7rqqqk 19 10 are be VBP cord-104269-9r7rqqqk 19 11 ct/3 ct/3 CD cord-104269-9r7rqqqk 19 12 heterodimers heterodimer NNS cord-104269-9r7rqqqk 19 13 , , , cord-104269-9r7rqqqk 19 14 but but CC cord-104269-9r7rqqqk 19 15 during during IN cord-104269-9r7rqqqk 19 16 their -PRON- PRP$ cord-104269-9r7rqqqk 19 17 intracellular intracellular JJ cord-104269-9r7rqqqk 19 18 transport transport NN cord-104269-9r7rqqqk 19 19 to to IN cord-104269-9r7rqqqk 19 20 the the DT cord-104269-9r7rqqqk 19 21 cell cell NN cord-104269-9r7rqqqk 19 22 surface surface NN cord-104269-9r7rqqqk 19 23 , , , cord-104269-9r7rqqqk 19 24 they -PRON- PRP cord-104269-9r7rqqqk 19 25 are be VBP cord-104269-9r7rqqqk 19 26 transiently transiently RB cord-104269-9r7rqqqk 19 27 associated associate VBN cord-104269-9r7rqqqk 19 28 with with IN cord-104269-9r7rqqqk 19 29 a a DT cord-104269-9r7rqqqk 19 30 third third JJ cord-104269-9r7rqqqk 19 31 nonpolymorphic nonpolymorphic JJ cord-104269-9r7rqqqk 19 32 polypeptide polypeptide NN cord-104269-9r7rqqqk 19 33 , , , cord-104269-9r7rqqqk 19 34 the the DT cord-104269-9r7rqqqk 19 35 invariant invariant JJ cord-104269-9r7rqqqk 19 36 chain chain NN cord-104269-9r7rqqqk 19 37 ( ( -LRB- cord-104269-9r7rqqqk 19 38 Ii Ii NNP cord-104269-9r7rqqqk 19 39 ) ) -RRB- cord-104269-9r7rqqqk 19 40 . . . cord-104269-9r7rqqqk 20 1 Ii Ii NNP cord-104269-9r7rqqqk 20 2 is be VBZ cord-104269-9r7rqqqk 20 3 a a DT cord-104269-9r7rqqqk 20 4 type type NN cord-104269-9r7rqqqk 20 5 II II NNP cord-104269-9r7rqqqk 20 6 membrane membrane NN cord-104269-9r7rqqqk 20 7 protein protein NN cord-104269-9r7rqqqk 20 8 with with IN cord-104269-9r7rqqqk 20 9 different different JJ cord-104269-9r7rqqqk 20 10 isoforms isoform NNS cord-104269-9r7rqqqk 20 11 that that WDT cord-104269-9r7rqqqk 20 12 arise arise VBP cord-104269-9r7rqqqk 20 13 from from IN cord-104269-9r7rqqqk 20 14 alternative alternative JJ cord-104269-9r7rqqqk 20 15 splicing splicing NN cord-104269-9r7rqqqk 20 16 of of IN cord-104269-9r7rqqqk 20 17 one one CD cord-104269-9r7rqqqk 20 18 exon exon NN cord-104269-9r7rqqqk 20 19 and and CC cord-104269-9r7rqqqk 20 20 the the DT cord-104269-9r7rqqqk 20 21 use use NN cord-104269-9r7rqqqk 20 22 of of IN cord-104269-9r7rqqqk 20 23 two two CD cord-104269-9r7rqqqk 20 24 translation translation NN cord-104269-9r7rqqqk 20 25 initiation initiation NN cord-104269-9r7rqqqk 20 26 sites site NNS cord-104269-9r7rqqqk 20 27 ( ( -LRB- cord-104269-9r7rqqqk 20 28 Strubin Strubin NNP cord-104269-9r7rqqqk 20 29 et et NNP cord-104269-9r7rqqqk 20 30 al al NNP cord-104269-9r7rqqqk 20 31 . . NNP cord-104269-9r7rqqqk 20 32 , , , cord-104269-9r7rqqqk 20 33 1986 1986 CD cord-104269-9r7rqqqk 20 34 ; ; . cord-104269-9r7rqqqk 21 1 O'Sullivan O'Sullivan NNP cord-104269-9r7rqqqk 21 2 et et NNP cord-104269-9r7rqqqk 21 3 al al NNP cord-104269-9r7rqqqk 21 4 . . . cord-104269-9r7rqqqk 22 1 , , , cord-104269-9r7rqqqk 22 2 1987 1987 CD cord-104269-9r7rqqqk 22 3 ; ; : cord-104269-9r7rqqqk 22 4 Koch Koch NNP cord-104269-9r7rqqqk 22 5 et et NNP cord-104269-9r7rqqqk 22 6 al al NNP cord-104269-9r7rqqqk 22 7 . . NNP cord-104269-9r7rqqqk 22 8 , , , cord-104269-9r7rqqqk 22 9 1987 1987 CD cord-104269-9r7rqqqk 22 10 ) ) -RRB- cord-104269-9r7rqqqk 22 11 . . . cord-104269-9r7rqqqk 23 1 Studies study NNS cord-104269-9r7rqqqk 23 2 of of IN cord-104269-9r7rqqqk 23 3 mutant mutant JJ cord-104269-9r7rqqqk 23 4 mice mouse NNS cord-104269-9r7rqqqk 23 5 lacking lack VBG cord-104269-9r7rqqqk 23 6 Ii Ii NNP cord-104269-9r7rqqqk 23 7 have have VBP cord-104269-9r7rqqqk 23 8 established establish VBN cord-104269-9r7rqqqk 23 9 that that IN cord-104269-9r7rqqqk 23 10 Ii Ii NNP cord-104269-9r7rqqqk 23 11 plays play VBZ cord-104269-9r7rqqqk 23 12 a a DT cord-104269-9r7rqqqk 23 13 critical critical JJ cord-104269-9r7rqqqk 23 14 role role NN cord-104269-9r7rqqqk 23 15 in in IN cord-104269-9r7rqqqk 23 16 the the DT cord-104269-9r7rqqqk 23 17 surface surface NN cord-104269-9r7rqqqk 23 18 expression expression NN cord-104269-9r7rqqqk 23 19 of of IN cord-104269-9r7rqqqk 23 20 class class NN cord-104269-9r7rqqqk 23 21 II ii CD cord-104269-9r7rqqqk 23 22 molecules molecule NNS cord-104269-9r7rqqqk 23 23 and and CC cord-104269-9r7rqqqk 23 24 in in IN cord-104269-9r7rqqqk 23 25 the the DT cord-104269-9r7rqqqk 23 26 ability ability NN cord-104269-9r7rqqqk 23 27 of of IN cord-104269-9r7rqqqk 23 28 class class NN cord-104269-9r7rqqqk 23 29 II ii CD cord-104269-9r7rqqqk 23 30 molecules molecule NNS cord-104269-9r7rqqqk 23 31 to to TO cord-104269-9r7rqqqk 23 32 present present VB cord-104269-9r7rqqqk 23 33 native native JJ cord-104269-9r7rqqqk 23 34 protein protein NN cord-104269-9r7rqqqk 23 35 antigens antigen NNS cord-104269-9r7rqqqk 23 36 ( ( -LRB- cord-104269-9r7rqqqk 23 37 Viville Viville NNP cord-104269-9r7rqqqk 23 38 et et NNP cord-104269-9r7rqqqk 23 39 al al NNP cord-104269-9r7rqqqk 23 40 . . NNP cord-104269-9r7rqqqk 23 41 , , , cord-104269-9r7rqqqk 23 42 1993 1993 CD cord-104269-9r7rqqqk 23 43 ; ; : cord-104269-9r7rqqqk 23 44 Bikoff Bikoff NNP cord-104269-9r7rqqqk 23 45 et et NNP cord-104269-9r7rqqqk 23 46 al al NNP cord-104269-9r7rqqqk 23 47 . . NNP cord-104269-9r7rqqqk 23 48 , , , cord-104269-9r7rqqqk 23 49 1993 1993 CD cord-104269-9r7rqqqk 23 50 ) ) -RRB- cord-104269-9r7rqqqk 23 51 .. .. . cord-104269-9r7rqqqk 24 1 Ii Ii NNP cord-104269-9r7rqqqk 24 2 determines determine VBZ cord-104269-9r7rqqqk 24 3 the the DT cord-104269-9r7rqqqk 24 4 membrane membrane NN cord-104269-9r7rqqqk 24 5 trafficking trafficking NN cord-104269-9r7rqqqk 24 6 of of IN cord-104269-9r7rqqqk 24 7 MHC MHC NNP cord-104269-9r7rqqqk 24 8 class class NN cord-104269-9r7rqqqk 24 9 II II NNP cord-104269-9r7rqqqk 24 10 a/3 a/3 NN cord-104269-9r7rqqqk 24 11 dimers dimer NNS cord-104269-9r7rqqqk 24 12 ( ( -LRB- cord-104269-9r7rqqqk 24 13 Bakke Bakke NNP cord-104269-9r7rqqqk 24 14 and and CC cord-104269-9r7rqqqk 24 15 Dobberstein Dobberstein NNP cord-104269-9r7rqqqk 24 16 , , , cord-104269-9r7rqqqk 24 17 1990 1990 CD cord-104269-9r7rqqqk 24 18 ; ; : cord-104269-9r7rqqqk 24 19 Lotteau Lotteau NNP cord-104269-9r7rqqqk 24 20 et et NNP cord-104269-9r7rqqqk 24 21 al al NNP cord-104269-9r7rqqqk 24 22 . . NNP cord-104269-9r7rqqqk 24 23 , , , cord-104269-9r7rqqqk 24 24 1990 1990 CD cord-104269-9r7rqqqk 24 25 ) ) -RRB- cord-104269-9r7rqqqk 24 26 and and CC cord-104269-9r7rqqqk 24 27 also also RB cord-104269-9r7rqqqk 24 28 blocks block VBZ cord-104269-9r7rqqqk 24 29 their -PRON- PRP$ cord-104269-9r7rqqqk 24 30 peptide peptide NN cord-104269-9r7rqqqk 24 31 - - HYPH cord-104269-9r7rqqqk 24 32 binding bind VBG cord-104269-9r7rqqqk 24 33 site site NN cord-104269-9r7rqqqk 24 34 during during IN cord-104269-9r7rqqqk 24 35 transit transit NN cord-104269-9r7rqqqk 24 36 through through IN cord-104269-9r7rqqqk 24 37 the the DT cord-104269-9r7rqqqk 24 38 biosynthetic biosynthetic JJ cord-104269-9r7rqqqk 24 39 pathway pathway NN cord-104269-9r7rqqqk 24 40 ( ( -LRB- cord-104269-9r7rqqqk 24 41 Roche Roche NNP cord-104269-9r7rqqqk 24 42 and and CC cord-104269-9r7rqqqk 24 43 Cresswell Cresswell NNP cord-104269-9r7rqqqk 24 44 , , , cord-104269-9r7rqqqk 24 45 1990 1990 CD cord-104269-9r7rqqqk 24 46 ; ; : cord-104269-9r7rqqqk 24 47 Teyton Teyton NNP cord-104269-9r7rqqqk 24 48 et et NNP cord-104269-9r7rqqqk 24 49 al al NNP cord-104269-9r7rqqqk 24 50 . . NNP cord-104269-9r7rqqqk 24 51 , , , cord-104269-9r7rqqqk 24 52 1990 1990 CD cord-104269-9r7rqqqk 24 53 ; ; : cord-104269-9r7rqqqk 25 1 Roche Roche NNP cord-104269-9r7rqqqk 25 2 et et NNP cord-104269-9r7rqqqk 25 3 al al NNP cord-104269-9r7rqqqk 25 4 . . NNP cord-104269-9r7rqqqk 25 5 , , , cord-104269-9r7rqqqk 25 6 1992 1992 CD cord-104269-9r7rqqqk 25 7 ) ) -RRB- cord-104269-9r7rqqqk 25 8 . . . cord-104269-9r7rqqqk 26 1 Ij Ij NNP cord-104269-9r7rqqqk 26 2 is be VBZ cord-104269-9r7rqqqk 26 3 assembled assemble VBN cord-104269-9r7rqqqk 26 4 with with IN cord-104269-9r7rqqqk 26 5 class class NN cord-104269-9r7rqqqk 26 6 II II NNP cord-104269-9r7rqqqk 26 7 a a NN cord-104269-9r7rqqqk 26 8 and and CC cord-104269-9r7rqqqk 26 9 13 13 CD cord-104269-9r7rqqqk 26 10 polypepfides polypepfide NNS cord-104269-9r7rqqqk 26 11 in in IN cord-104269-9r7rqqqk 26 12 the the DT cord-104269-9r7rqqqk 26 13 endoplasmic endoplasmic NN cord-104269-9r7rqqqk 26 14 reticulum reticulum NN cord-104269-9r7rqqqk 26 15 , , , cord-104269-9r7rqqqk 26 16 and and CC cord-104269-9r7rqqqk 26 17 , , , cord-104269-9r7rqqqk 26 18 after after IN cord-104269-9r7rqqqk 26 19 its -PRON- PRP$ cord-104269-9r7rqqqk 26 20 transport transport NN cord-104269-9r7rqqqk 26 21 through through IN cord-104269-9r7rqqqk 26 22 the the DT cord-104269-9r7rqqqk 26 23 Golgi Golgi NNP cord-104269-9r7rqqqk 26 24 region region NN cord-104269-9r7rqqqk 26 25 , , , cord-104269-9r7rqqqk 26 26 the the DT cord-104269-9r7rqqqk 26 27 a/3Ii a/3Ii NNP cord-104269-9r7rqqqk 26 28 complex complex NN cord-104269-9r7rqqqk 26 29 is be VBZ cord-104269-9r7rqqqk 26 30 sorted sort VBN cord-104269-9r7rqqqk 26 31 out out IN cord-104269-9r7rqqqk 26 32 of of IN cord-104269-9r7rqqqk 26 33 the the DT cord-104269-9r7rqqqk 26 34 constitutive constitutive JJ cord-104269-9r7rqqqk 26 35 biosynthetic biosynthetic JJ cord-104269-9r7rqqqk 26 36 pathway pathway NN cord-104269-9r7rqqqk 26 37 to to IN cord-104269-9r7rqqqk 26 38 an an DT cord-104269-9r7rqqqk 26 39 acidic acidic JJ cord-104269-9r7rqqqk 26 40 compartment compartment NN cord-104269-9r7rqqqk 26 41 within within IN cord-104269-9r7rqqqk 26 42 the the DT cord-104269-9r7rqqqk 26 43 endocytic endocytic JJ cord-104269-9r7rqqqk 26 44 system system NN cord-104269-9r7rqqqk 26 45 ( ( -LRB- cord-104269-9r7rqqqk 26 46 Cresswell Cresswell NNP cord-104269-9r7rqqqk 26 47 , , , cord-104269-9r7rqqqk 26 48 1985 1985 CD cord-104269-9r7rqqqk 26 49 ; ; : cord-104269-9r7rqqqk 27 1 Lamb Lamb NNP cord-104269-9r7rqqqk 27 2 et et NNP cord-104269-9r7rqqqk 27 3 al al NNP cord-104269-9r7rqqqk 27 4 . . NNP cord-104269-9r7rqqqk 27 5 , , , cord-104269-9r7rqqqk 27 6 1991 1991 CD cord-104269-9r7rqqqk 27 7 ; ; : cord-104269-9r7rqqqk 27 8 Pieters Pieters NNP cord-104269-9r7rqqqk 27 9 et et NNP cord-104269-9r7rqqqk 27 10 al al NNP cord-104269-9r7rqqqk 27 11 . . NNP cord-104269-9r7rqqqk 27 12 , , , cord-104269-9r7rqqqk 27 13 1991 1991 CD cord-104269-9r7rqqqk 27 14 ) ) -RRB- cord-104269-9r7rqqqk 27 15 . . . cord-104269-9r7rqqqk 28 1 Within within IN cord-104269-9r7rqqqk 28 2 this this DT cord-104269-9r7rqqqk 28 3 prelysosomal prelysosomal NN cord-104269-9r7rqqqk 28 4 ( ( -LRB- cord-104269-9r7rqqqk 28 5 Peters Peters NNP cord-104269-9r7rqqqk 28 6 et et NNP cord-104269-9r7rqqqk 28 7 al al NNP cord-104269-9r7rqqqk 28 8 . . NNP cord-104269-9r7rqqqk 28 9 , , , cord-104269-9r7rqqqk 28 10 1991 1991 CD cord-104269-9r7rqqqk 28 11 ) ) -RRB- cord-104269-9r7rqqqk 28 12 , , , cord-104269-9r7rqqqk 28 13 early early JJ cord-104269-9r7rqqqk 28 14 lysosomal lysosomal NN cord-104269-9r7rqqqk 28 15 ( ( -LRB- cord-104269-9r7rqqqk 28 16 Harding Harding NNP cord-104269-9r7rqqqk 28 17 and and CC cord-104269-9r7rqqqk 28 18 Geuze Geuze NNP cord-104269-9r7rqqqk 28 19 , , , cord-104269-9r7rqqqk 28 20 1993 1993 CD cord-104269-9r7rqqqk 28 21 ) ) -RRB- cord-104269-9r7rqqqk 28 22 , , , cord-104269-9r7rqqqk 28 23 or or CC cord-104269-9r7rqqqk 28 24 endosomal endosomal NNP cord-104269-9r7rqqqk 28 25 com com NNP cord-104269-9r7rqqqk 28 26 - - HYPH cord-104269-9r7rqqqk 28 27 pa pa NNP cord-104269-9r7rqqqk 29 1 ~i ~i NNP cord-104269-9r7rqqqk 29 2 , , , cord-104269-9r7rqqqk 29 3 uent uent JJ cord-104269-9r7rqqqk 29 4 ( ( -LRB- cord-104269-9r7rqqqk 29 5 Guagliardi Guagliardi NNPS cord-104269-9r7rqqqk 29 6 et et NNP cord-104269-9r7rqqqk 29 7 al al NNP cord-104269-9r7rqqqk 29 8 . . NNP cord-104269-9r7rqqqk 29 9 , , , cord-104269-9r7rqqqk 29 10 1990 1990 CD cord-104269-9r7rqqqk 29 11 ) ) -RRB- cord-104269-9r7rqqqk 30 1 , , , cord-104269-9r7rqqqk 30 2 Ii Ii NNP cord-104269-9r7rqqqk 30 3 dissociates dissociate VBZ cord-104269-9r7rqqqk 30 4 from from IN cord-104269-9r7rqqqk 30 5 class class NN cord-104269-9r7rqqqk 30 6 II II NNP cord-104269-9r7rqqqk 30 7 a/3 a/3 NN cord-104269-9r7rqqqk 30 8 dimers dimer NNS cord-104269-9r7rqqqk 30 9 , , , cord-104269-9r7rqqqk 30 10 permitting permit VBG cord-104269-9r7rqqqk 30 11 binding binding NN cord-104269-9r7rqqqk 30 12 ofpeptides ofpeptide NNS cord-104269-9r7rqqqk 30 13 from from IN cord-104269-9r7rqqqk 30 14 exogenous exogenous JJ cord-104269-9r7rqqqk 30 15 foreign foreign JJ cord-104269-9r7rqqqk 30 16 antigens antigen NNS cord-104269-9r7rqqqk 30 17 generated generate VBN cord-104269-9r7rqqqk 30 18 in in IN cord-104269-9r7rqqqk 30 19 the the DT cord-104269-9r7rqqqk 30 20 endocytic endocytic JJ cord-104269-9r7rqqqk 30 21 pathway pathway NN cord-104269-9r7rqqqk 30 22 . . . cord-104269-9r7rqqqk 31 1 Class class NN cord-104269-9r7rqqqk 31 2 II II NNP cord-104269-9r7rqqqk 31 3 a/3 a/3 NN cord-104269-9r7rqqqk 31 4 dimers dimer NNS cord-104269-9r7rqqqk 31 5 loaded load VBN cord-104269-9r7rqqqk 31 6 with with IN cord-104269-9r7rqqqk 31 7 peptide peptide NN cord-104269-9r7rqqqk 31 8 are be VBP cord-104269-9r7rqqqk 31 9 then then RB cord-104269-9r7rqqqk 31 10 transported transport VBN cord-104269-9r7rqqqk 31 11 to to IN cord-104269-9r7rqqqk 31 12 the the DT cord-104269-9r7rqqqk 31 13 cell cell NN cord-104269-9r7rqqqk 31 14 surface surface NN cord-104269-9r7rqqqk 31 15 , , , cord-104269-9r7rqqqk 31 16 while while IN cord-104269-9r7rqqqk 31 17 the the DT cord-104269-9r7rqqqk 31 18 free free JJ cord-104269-9r7rqqqk 31 19 Ii Ii NNP cord-104269-9r7rqqqk 31 20 is be VBZ cord-104269-9r7rqqqk 31 21 rapidly rapidly RB cord-104269-9r7rqqqk 31 22 degraded degrade VBN cord-104269-9r7rqqqk 31 23 ( ( -LRB- cord-104269-9r7rqqqk 31 24 Blum Blum NNP cord-104269-9r7rqqqk 31 25 and and CC cord-104269-9r7rqqqk 31 26 Cresswell Cresswell NNP cord-104269-9r7rqqqk 31 27 , , , cord-104269-9r7rqqqk 31 28 1988 1988 CD cord-104269-9r7rqqqk 31 29 ; ; . cord-104269-9r7rqqqk 32 1 Pieters Pieters NNP cord-104269-9r7rqqqk 32 2 et et NNP cord-104269-9r7rqqqk 32 3 al al NNP cord-104269-9r7rqqqk 32 4 . . NNP cord-104269-9r7rqqqk 32 5 , , , cord-104269-9r7rqqqk 32 6 1991 1991 CD cord-104269-9r7rqqqk 32 7 ) ) -RRB- cord-104269-9r7rqqqk 32 8 . . . cord-104269-9r7rqqqk 33 1 Although although IN cord-104269-9r7rqqqk 33 2 a/3Ii a/3Ii NNP cord-104269-9r7rqqqk 33 3 complexes complex NNS cord-104269-9r7rqqqk 33 4 are be VBP cord-104269-9r7rqqqk 33 5 thought think VBN cord-104269-9r7rqqqk 33 6 to to TO cord-104269-9r7rqqqk 33 7 be be VB cord-104269-9r7rqqqk 33 8 delivered deliver VBN cord-104269-9r7rqqqk 33 9 to to IN cord-104269-9r7rqqqk 33 10 an an DT cord-104269-9r7rqqqk 33 11 endocytic endocytic JJ cord-104269-9r7rqqqk 33 12 compartment compartment NN cord-104269-9r7rqqqk 33 13 predominantly predominantly RB cord-104269-9r7rqqqk 33 14 by by IN cord-104269-9r7rqqqk 33 15 an an DT cord-104269-9r7rqqqk 33 16 intracellular intracellular JJ cord-104269-9r7rqqqk 33 17 mute mute JJ cord-104269-9r7rqqqk 33 18 , , , cord-104269-9r7rqqqk 33 19 small small JJ cord-104269-9r7rqqqk 33 20 amounts amount NNS cord-104269-9r7rqqqk 33 21 of of IN cord-104269-9r7rqqqk 33 22 Ii Ii NNP cord-104269-9r7rqqqk 33 23 and and CC cord-104269-9r7rqqqk 33 24 aflli aflli NNP cord-104269-9r7rqqqk 33 25 complexes complex NNS cord-104269-9r7rqqqk 33 26 have have VBP cord-104269-9r7rqqqk 33 27 been be VBN cord-104269-9r7rqqqk 33 28 detected detect VBN cord-104269-9r7rqqqk 33 29 on on IN cord-104269-9r7rqqqk 33 30 the the DT cord-104269-9r7rqqqk 33 31 cell cell NN cord-104269-9r7rqqqk 33 32 surface surface NN cord-104269-9r7rqqqk 33 33 , , , cord-104269-9r7rqqqk 33 34 and and CC cord-104269-9r7rqqqk 33 35 rapid rapid JJ cord-104269-9r7rqqqk 33 36 internalization internalization NN cord-104269-9r7rqqqk 33 37 of of IN cord-104269-9r7rqqqk 33 38 a/3Ii a/3Ii NNP cord-104269-9r7rqqqk 33 39 complexes complex NNS cord-104269-9r7rqqqk 33 40 has have VBZ cord-104269-9r7rqqqk 33 41 been be VBN cord-104269-9r7rqqqk 33 42 reported report VBN cord-104269-9r7rqqqk 34 1 ( ( -LRB- cord-104269-9r7rqqqk 34 2 Wraight Wraight NNP cord-104269-9r7rqqqk 34 3 et et NNP cord-104269-9r7rqqqk 34 4 al al NNP cord-104269-9r7rqqqk 34 5 . . NNP cord-104269-9r7rqqqk 34 6 , , , cord-104269-9r7rqqqk 34 7 1990 1990 CD cord-104269-9r7rqqqk 35 1 ; ; : cord-104269-9r7rqqqk 35 2 Lotteau Lotteau NNP cord-104269-9r7rqqqk 35 3 et et NNP cord-104269-9r7rqqqk 35 4 al al NNP cord-104269-9r7rqqqk 35 5 . . NNP cord-104269-9r7rqqqk 35 6 , , , cord-104269-9r7rqqqk 35 7 1990 1990 CD cord-104269-9r7rqqqk 35 8 ; ; : cord-104269-9r7rqqqk 35 9 Roche Roche NNP cord-104269-9r7rqqqk 35 10 et et NNP cord-104269-9r7rqqqk 35 11 al al NNP cord-104269-9r7rqqqk 35 12 . . NNP cord-104269-9r7rqqqk 35 13 , , , cord-104269-9r7rqqqk 35 14 1993 1993 CD cord-104269-9r7rqqqk 35 15 ) ) -RRB- cord-104269-9r7rqqqk 35 16 . . . cord-104269-9r7rqqqk 36 1 These these DT cord-104269-9r7rqqqk 36 2 data datum NNS cord-104269-9r7rqqqk 36 3 suggest suggest VBP cord-104269-9r7rqqqk 36 4 that that IN cord-104269-9r7rqqqk 36 5 at at IN cord-104269-9r7rqqqk 36 6 least least JJS cord-104269-9r7rqqqk 36 7 some some DT cord-104269-9r7rqqqk 36 8 newly newly RB cord-104269-9r7rqqqk 36 9 synthesized synthesize VBN cord-104269-9r7rqqqk 36 10 li li NNP cord-104269-9r7rqqqk 36 11 molecules molecule NNS cord-104269-9r7rqqqk 36 12 are be VBP cord-104269-9r7rqqqk 36 13 transported transport VBN cord-104269-9r7rqqqk 36 14 to to IN cord-104269-9r7rqqqk 36 15 an an DT cord-104269-9r7rqqqk 36 16 cndocytic cndocytic JJ cord-104269-9r7rqqqk 36 17 compartment compartment NN cord-104269-9r7rqqqk 36 18 via via IN cord-104269-9r7rqqqk 36 19 the the DT cord-104269-9r7rqqqk 36 20 plasma plasma NN cord-104269-9r7rqqqk 36 21 membrane membrane NN cord-104269-9r7rqqqk 36 22 , , , cord-104269-9r7rqqqk 36 23 a a DT cord-104269-9r7rqqqk 36 24 route route NN cord-104269-9r7rqqqk 36 25 analogous analogous JJ cord-104269-9r7rqqqk 36 26 to to IN cord-104269-9r7rqqqk 36 27 that that DT cord-104269-9r7rqqqk 36 28 taken take VBN cord-104269-9r7rqqqk 36 29 by by IN cord-104269-9r7rqqqk 36 30 some some DT cord-104269-9r7rqqqk 36 31 integral integral JJ cord-104269-9r7rqqqk 36 32 lysosomal lysosomal JJ cord-104269-9r7rqqqk 36 33 membrane membrane NN cord-104269-9r7rqqqk 36 34 proteins protein NNS cord-104269-9r7rqqqk 36 35 , , , cord-104269-9r7rqqqk 36 36 e.g. e.g. RB cord-104269-9r7rqqqk 36 37 , , , cord-104269-9r7rqqqk 36 38 lysosomal lysosomal JJ cord-104269-9r7rqqqk 36 39 acid acid NN cord-104269-9r7rqqqk 36 40 phosphatase phosphatase NN cord-104269-9r7rqqqk 36 41 ( ( -LRB- cord-104269-9r7rqqqk 36 42 Braun Braun NNP cord-104269-9r7rqqqk 36 43 et et NNP cord-104269-9r7rqqqk 36 44 al al NNP cord-104269-9r7rqqqk 36 45 . . NNP cord-104269-9r7rqqqk 36 46 , , , cord-104269-9r7rqqqk 36 47 1989 1989 CD cord-104269-9r7rqqqk 36 48 ; ; : cord-104269-9r7rqqqk 36 49 Peters Peters NNP cord-104269-9r7rqqqk 36 50 et et NNP cord-104269-9r7rqqqk 36 51 al al NNP cord-104269-9r7rqqqk 36 52 . . NNP cord-104269-9r7rqqqk 36 53 , , , cord-104269-9r7rqqqk 36 54 1990 1990 CD cord-104269-9r7rqqqk 36 55 ) ) -RRB- cord-104269-9r7rqqqk 36 56 . . . cord-104269-9r7rqqqk 37 1 li li NNP cord-104269-9r7rqqqk 37 2 contains contain VBZ cord-104269-9r7rqqqk 37 3 one one CD cord-104269-9r7rqqqk 37 4 or or CC cord-104269-9r7rqqqk 37 5 more more JJR cord-104269-9r7rqqqk 37 6 sorting sorting NN cord-104269-9r7rqqqk 37 7 signals signal NNS cord-104269-9r7rqqqk 37 8 within within IN cord-104269-9r7rqqqk 37 9 its -PRON- PRP$ cord-104269-9r7rqqqk 37 10 aminoterminal aminoterminal JJ cord-104269-9r7rqqqk 37 11 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 37 12 domain domain NN cord-104269-9r7rqqqk 37 13 required require VBN cord-104269-9r7rqqqk 37 14 for for IN cord-104269-9r7rqqqk 37 15 localization localization NN cord-104269-9r7rqqqk 37 16 to to IN cord-104269-9r7rqqqk 37 17 the the DT cord-104269-9r7rqqqk 37 18 cndocytic cndocytic JJ cord-104269-9r7rqqqk 37 19 system system NN cord-104269-9r7rqqqk 37 20 ( ( -LRB- cord-104269-9r7rqqqk 37 21 Bakke Bakke NNP cord-104269-9r7rqqqk 37 22 and and CC cord-104269-9r7rqqqk 37 23 Dobberstcin Dobberstcin NNP cord-104269-9r7rqqqk 37 24 , , , cord-104269-9r7rqqqk 37 25 1990 1990 CD cord-104269-9r7rqqqk 37 26 ; ; : cord-104269-9r7rqqqk 38 1 Lotteau Lotteau NNP cord-104269-9r7rqqqk 38 2 et et NNP cord-104269-9r7rqqqk 38 3 al al NNP cord-104269-9r7rqqqk 38 4 . . . cord-104269-9r7rqqqk 39 1 , , , cord-104269-9r7rqqqk 39 2 1990 1990 CD cord-104269-9r7rqqqk 39 3 ; ; : cord-104269-9r7rqqqk 39 4 Simonsen Simonsen NNP cord-104269-9r7rqqqk 39 5 ct ct NNP cord-104269-9r7rqqqk 39 6 al al NNP cord-104269-9r7rqqqk 39 7 . . NNP cord-104269-9r7rqqqk 39 8 , , , cord-104269-9r7rqqqk 39 9 1993 1993 CD cord-104269-9r7rqqqk 39 10 ) ) -RRB- cord-104269-9r7rqqqk 39 11 . . . cord-104269-9r7rqqqk 40 1 Ifli Ifli VBN cord-104269-9r7rqqqk 40 2 with with IN cord-104269-9r7rqqqk 40 3 a a DT cord-104269-9r7rqqqk 40 4 truncated truncated JJ cord-104269-9r7rqqqk 40 5 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 40 6 domain domain NN cord-104269-9r7rqqqk 40 7 is be VBZ cord-104269-9r7rqqqk 40 8 coexpressed coexpresse VBN cord-104269-9r7rqqqk 40 9 with with IN cord-104269-9r7rqqqk 40 10 class class NN cord-104269-9r7rqqqk 40 11 II II NNP cord-104269-9r7rqqqk 40 12 o~/~ o~/~ NNS cord-104269-9r7rqqqk 40 13 dimers dimer NNS cord-104269-9r7rqqqk 40 14 , , , cord-104269-9r7rqqqk 40 15 li li NNP cord-104269-9r7rqqqk 40 16 fails fail VBZ cord-104269-9r7rqqqk 40 17 to to TO cord-104269-9r7rqqqk 40 18 dissociate dissociate VB cord-104269-9r7rqqqk 40 19 , , , cord-104269-9r7rqqqk 40 20 and and CC cord-104269-9r7rqqqk 40 21 a a DT cord-104269-9r7rqqqk 40 22 ~ ~ CD cord-104269-9r7rqqqk 40 23 Ii Ii NNP cord-104269-9r7rqqqk 40 24 complexes complex NNS cord-104269-9r7rqqqk 40 25 are be VBP cord-104269-9r7rqqqk 40 26 expressed express VBN cord-104269-9r7rqqqk 40 27 on on IN cord-104269-9r7rqqqk 40 28 the the DT cord-104269-9r7rqqqk 40 29 cell cell NN cord-104269-9r7rqqqk 40 30 surface surface NN cord-104269-9r7rqqqk 40 31 ( ( -LRB- cord-104269-9r7rqqqk 40 32 Roche Roche NNP cord-104269-9r7rqqqk 40 33 et et NNP cord-104269-9r7rqqqk 40 34 al al NNP cord-104269-9r7rqqqk 40 35 . . NNP cord-104269-9r7rqqqk 40 36 , , , cord-104269-9r7rqqqk 40 37 1992 1992 CD cord-104269-9r7rqqqk 40 38 ) ) -RRB- cord-104269-9r7rqqqk 40 39 . . . cord-104269-9r7rqqqk 41 1 However however RB cord-104269-9r7rqqqk 41 2 , , , cord-104269-9r7rqqqk 41 3 the the DT cord-104269-9r7rqqqk 41 4 sorting sorting NN cord-104269-9r7rqqqk 41 5 signals signal NNS cord-104269-9r7rqqqk 41 6 within within IN cord-104269-9r7rqqqk 41 7 the the DT cord-104269-9r7rqqqk 41 8 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 41 9 domain domain NN cord-104269-9r7rqqqk 41 10 of of IN cord-104269-9r7rqqqk 41 11 li li NNP cord-104269-9r7rqqqk 41 12 have have VBP cord-104269-9r7rqqqk 41 13 not not RB cord-104269-9r7rqqqk 41 14 been be VBN cord-104269-9r7rqqqk 41 15 wellcharacterized wellcharacterize VBN cord-104269-9r7rqqqk 41 16 , , , cord-104269-9r7rqqqk 41 17 and and CC cord-104269-9r7rqqqk 41 18 neither neither CC cord-104269-9r7rqqqk 41 19 the the DT cord-104269-9r7rqqqk 41 20 trafficking trafficking NN cord-104269-9r7rqqqk 41 21 pathway pathway NN cord-104269-9r7rqqqk 41 22 taken take VBN cord-104269-9r7rqqqk 41 23 by by IN cord-104269-9r7rqqqk 41 24 MHC MHC NNP cord-104269-9r7rqqqk 41 25 class class NN cord-104269-9r7rqqqk 41 26 II II NNP cord-104269-9r7rqqqk 41 27 cd3Ii cd3Ii NNP cord-104269-9r7rqqqk 41 28 complexes complex NNS cord-104269-9r7rqqqk 41 29 nor nor CC cord-104269-9r7rqqqk 41 30 the the DT cord-104269-9r7rqqqk 41 31 cndocytic cndocytic JJ cord-104269-9r7rqqqk 41 32 membrane membrane NN cord-104269-9r7rqqqk 41 33 compartment compartment NN cord-104269-9r7rqqqk 41 34 where where WRB cord-104269-9r7rqqqk 41 35 o~/~ o~/~ VBP cord-104269-9r7rqqqk 41 36 dimcrs dimcrs JJ cord-104269-9r7rqqqk 41 37 encounter encounter NN cord-104269-9r7rqqqk 41 38 antigen antigen NN cord-104269-9r7rqqqk 41 39 have have VBP cord-104269-9r7rqqqk 41 40 been be VBN cord-104269-9r7rqqqk 41 41 clearly clearly RB cord-104269-9r7rqqqk 41 42 defined define VBN cord-104269-9r7rqqqk 41 43 . . . cord-104269-9r7rqqqk 42 1 In in IN cord-104269-9r7rqqqk 42 2 this this DT cord-104269-9r7rqqqk 42 3 study study NN cord-104269-9r7rqqqk 42 4 , , , cord-104269-9r7rqqqk 42 5 we -PRON- PRP cord-104269-9r7rqqqk 42 6 have have VBP cord-104269-9r7rqqqk 42 7 constructed construct VBN cord-104269-9r7rqqqk 42 8 Ii Ii NNP cord-104269-9r7rqqqk 42 9 - - HYPH cord-104269-9r7rqqqk 42 10 human human JJ cord-104269-9r7rqqqk 42 11 transferrin transferrin NN cord-104269-9r7rqqqk 42 12 receptor receptor NN cord-104269-9r7rqqqk 42 13 ( ( -LRB- cord-104269-9r7rqqqk 42 14 TR TR NNP cord-104269-9r7rqqqk 42 15 ) ) -RRB- cord-104269-9r7rqqqk 42 16 chimeras chimera NNS cord-104269-9r7rqqqk 42 17 with with IN cord-104269-9r7rqqqk 42 18 the the DT cord-104269-9r7rqqqk 42 19 aim aim NN cord-104269-9r7rqqqk 42 20 of of IN cord-104269-9r7rqqqk 42 21 characterizing characterize VBG cord-104269-9r7rqqqk 42 22 li li NNP cord-104269-9r7rqqqk 42 23 sorting sort VBG cord-104269-9r7rqqqk 42 24 signals signal NNS cord-104269-9r7rqqqk 42 25 . . . cord-104269-9r7rqqqk 43 1 We -PRON- PRP cord-104269-9r7rqqqk 43 2 show show VBP cord-104269-9r7rqqqk 43 3 that that IN cord-104269-9r7rqqqk 43 4 the the DT cord-104269-9r7rqqqk 43 5 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 43 6 domain domain NN cord-104269-9r7rqqqk 43 7 of of IN cord-104269-9r7rqqqk 43 8 li li NNP cord-104269-9r7rqqqk 43 9 contains contain VBZ cord-104269-9r7rqqqk 43 10 two two CD cord-104269-9r7rqqqk 43 11 independent independent JJ cord-104269-9r7rqqqk 43 12 non non JJ cord-104269-9r7rqqqk 43 13 - - JJ cord-104269-9r7rqqqk 43 14 tyrosine tyrosine NN cord-104269-9r7rqqqk 43 15 - - HYPH cord-104269-9r7rqqqk 43 16 based base VBN cord-104269-9r7rqqqk 43 17 sorting sorting NN cord-104269-9r7rqqqk 43 18 signals signal NNS cord-104269-9r7rqqqk 43 19 , , , cord-104269-9r7rqqqk 43 20 both both DT cord-104269-9r7rqqqk 43 21 of of IN cord-104269-9r7rqqqk 43 22 which which WDT cord-104269-9r7rqqqk 43 23 mediate mediate VBP cord-104269-9r7rqqqk 43 24 internalization internalization NN cord-104269-9r7rqqqk 43 25 via via IN cord-104269-9r7rqqqk 43 26 clathrincoated clathrincoated JJ cord-104269-9r7rqqqk 43 27 pits pit NNS cord-104269-9r7rqqqk 43 28 of of IN cord-104269-9r7rqqqk 43 29 the the DT cord-104269-9r7rqqqk 43 30 plasma plasma NN cord-104269-9r7rqqqk 43 31 membrane membrane NN cord-104269-9r7rqqqk 43 32 . . . cord-104269-9r7rqqqk 44 1 Both both DT cord-104269-9r7rqqqk 44 2 signals signal NNS cord-104269-9r7rqqqk 44 3 contain contain VBP cord-104269-9r7rqqqk 44 4 two two CD cord-104269-9r7rqqqk 44 5 adjacent adjacent JJ cord-104269-9r7rqqqk 44 6 large large JJ cord-104269-9r7rqqqk 44 7 hydrophobic hydrophobic JJ cord-104269-9r7rqqqk 44 8 nonaromatic nonaromatic JJ cord-104269-9r7rqqqk 44 9 residues residue NNS cord-104269-9r7rqqqk 44 10 suggesting suggest VBG cord-104269-9r7rqqqk 44 11 that that IN cord-104269-9r7rqqqk 44 12 this this DT cord-104269-9r7rqqqk 44 13 may may MD cord-104269-9r7rqqqk 44 14 be be VB cord-104269-9r7rqqqk 44 15 a a DT cord-104269-9r7rqqqk 44 16 common common JJ cord-104269-9r7rqqqk 44 17 feature feature NN cord-104269-9r7rqqqk 44 18 of of IN cord-104269-9r7rqqqk 44 19 non non JJ cord-104269-9r7rqqqk 44 20 - - JJ cord-104269-9r7rqqqk 44 21 tyrosinebased tyrosinebased JJ cord-104269-9r7rqqqk 44 22 sorting sorting NN cord-104269-9r7rqqqk 44 23 signals signal NNS cord-104269-9r7rqqqk 44 24 . . . cord-104269-9r7rqqqk 45 1 Trafficking trafficking NN cord-104269-9r7rqqqk 45 2 of of IN cord-104269-9r7rqqqk 45 3 Ii Ii NNP cord-104269-9r7rqqqk 45 4 - - HYPH cord-104269-9r7rqqqk 45 5 TR TR NNP cord-104269-9r7rqqqk 45 6 chimeras chimera NNS cord-104269-9r7rqqqk 45 7 to to IN cord-104269-9r7rqqqk 45 8 an an DT cord-104269-9r7rqqqk 45 9 cndocytic cndocytic JJ cord-104269-9r7rqqqk 45 10 compartment compartment NN cord-104269-9r7rqqqk 45 11 where where WRB cord-104269-9r7rqqqk 45 12 they -PRON- PRP cord-104269-9r7rqqqk 45 13 are be VBP cord-104269-9r7rqqqk 45 14 degraded degrade VBN cord-104269-9r7rqqqk 45 15 is be VBZ cord-104269-9r7rqqqk 45 16 dependent dependent JJ cord-104269-9r7rqqqk 45 17 upon upon IN cord-104269-9r7rqqqk 45 18 these these DT cord-104269-9r7rqqqk 45 19 signals signal NNS cord-104269-9r7rqqqk 45 20 but but CC cord-104269-9r7rqqqk 45 21 occurs occur VBZ cord-104269-9r7rqqqk 45 22 predominantly predominantly RB cord-104269-9r7rqqqk 45 23 by by IN cord-104269-9r7rqqqk 45 24 a a DT cord-104269-9r7rqqqk 45 25 direct direct JJ cord-104269-9r7rqqqk 45 26 intracclhlar intracclhlar NNP cord-104269-9r7rqqqk 45 27 route route NN cord-104269-9r7rqqqk 45 28 , , , cord-104269-9r7rqqqk 45 29 implying imply VBG cord-104269-9r7rqqqk 45 30 that that IN cord-104269-9r7rqqqk 45 31 the the DT cord-104269-9r7rqqqk 45 32 non non JJ cord-104269-9r7rqqqk 45 33 - - JJ cord-104269-9r7rqqqk 45 34 tyrosine tyrosine NN cord-104269-9r7rqqqk 45 35 - - HYPH cord-104269-9r7rqqqk 45 36 based base VBN cord-104269-9r7rqqqk 45 37 sorting sorting NN cord-104269-9r7rqqqk 45 38 signals signal NNS cord-104269-9r7rqqqk 45 39 arc arc NNP cord-104269-9r7rqqqk 45 40 efficiently efficiently RB cord-104269-9r7rqqqk 45 41 recognized recognize VBN cord-104269-9r7rqqqk 45 42 by by IN cord-104269-9r7rqqqk 45 43 sorting sort VBG cord-104269-9r7rqqqk 45 44 machinery machinery NN cord-104269-9r7rqqqk 45 45 located locate VBN cord-104269-9r7rqqqk 45 46 in in IN cord-104269-9r7rqqqk 45 47 the the DT cord-104269-9r7rqqqk 45 48 trans trans NNP cord-104269-9r7rqqqk 45 49 - - NNP cord-104269-9r7rqqqk 45 50 Golgi Golgi NNP cord-104269-9r7rqqqk 45 51 . . . cord-104269-9r7rqqqk 46 1 This this DT cord-104269-9r7rqqqk 46 2 cndocytic cndocytic JJ cord-104269-9r7rqqqk 46 3 compartment compartment NN cord-104269-9r7rqqqk 46 4 is be VBZ cord-104269-9r7rqqqk 46 5 distinct distinct JJ cord-104269-9r7rqqqk 46 6 from from IN cord-104269-9r7rqqqk 46 7 the the DT cord-104269-9r7rqqqk 46 8 sorting sorting NN cord-104269-9r7rqqqk 46 9 and and CC cord-104269-9r7rqqqk 46 10 recycling recycling NN cord-104269-9r7rqqqk 46 11 endosomal endosomal NNP cord-104269-9r7rqqqk 46 12 compartments compartment NNS cord-104269-9r7rqqqk 46 13 traversed traverse VBN cord-104269-9r7rqqqk 46 14 by by IN cord-104269-9r7rqqqk 46 15 the the DT cord-104269-9r7rqqqk 46 16 wild wild JJ cord-104269-9r7rqqqk 46 17 - - HYPH cord-104269-9r7rqqqk 46 18 type type NN cord-104269-9r7rqqqk 46 19 TR tr NN cord-104269-9r7rqqqk 46 20 , , , cord-104269-9r7rqqqk 46 21 and and CC cord-104269-9r7rqqqk 46 22 , , , cord-104269-9r7rqqqk 46 23 therefore therefore RB cord-104269-9r7rqqqk 46 24 , , , cord-104269-9r7rqqqk 46 25 is be VBZ cord-104269-9r7rqqqk 46 26 located locate VBN cord-104269-9r7rqqqk 46 27 within within IN cord-104269-9r7rqqqk 46 28 the the DT cord-104269-9r7rqqqk 46 29 prelysosomal prelysosomal NN cord-104269-9r7rqqqk 46 30 / / SYM cord-104269-9r7rqqqk 46 31 lysosomal lysosomal JJ cord-104269-9r7rqqqk 46 32 branch branch NN cord-104269-9r7rqqqk 46 33 of of IN cord-104269-9r7rqqqk 46 34 the the DT cord-104269-9r7rqqqk 46 35 endocytic endocytic JJ cord-104269-9r7rqqqk 46 36 pathway pathway NN cord-104269-9r7rqqqk 46 37 . . . cord-104269-9r7rqqqk 47 1 In in IN cord-104269-9r7rqqqk 47 2 addition addition NN cord-104269-9r7rqqqk 47 3 , , , cord-104269-9r7rqqqk 47 4 wc wc NNP cord-104269-9r7rqqqk 47 5 show show VBP cord-104269-9r7rqqqk 47 6 that that IN cord-104269-9r7rqqqk 47 7 efficient efficient JJ cord-104269-9r7rqqqk 47 8 delivery delivery NN cord-104269-9r7rqqqk 47 9 of of IN cord-104269-9r7rqqqk 47 10 Ii Ii NNP cord-104269-9r7rqqqk 47 11 - - HYPH cord-104269-9r7rqqqk 47 12 TR TR NNP cord-104269-9r7rqqqk 47 13 chimeras chimera NNS cord-104269-9r7rqqqk 47 14 to to IN cord-104269-9r7rqqqk 47 15 this this DT cord-104269-9r7rqqqk 47 16 endocytic endocytic JJ cord-104269-9r7rqqqk 47 17 compartment compartment NN cord-104269-9r7rqqqk 47 18 requires require VBZ cord-104269-9r7rqqqk 47 19 an an DT cord-104269-9r7rqqqk 47 20 independent independent JJ cord-104269-9r7rqqqk 47 21 signal signal NN cord-104269-9r7rqqqk 47 22 in in IN cord-104269-9r7rqqqk 47 23 the the DT cord-104269-9r7rqqqk 47 24 Ii Ii NNP cord-104269-9r7rqqqk 47 25 transmembrane transmembrane NNP cord-104269-9r7rqqqk 47 26 region region NN cord-104269-9r7rqqqk 47 27 . . . cord-104269-9r7rqqqk 48 1 A a DT cord-104269-9r7rqqqk 48 2 Cla Cla NNP cord-104269-9r7rqqqk 48 3 I -PRON- PRP cord-104269-9r7rqqqk 48 4 fragment fragment VBP cord-104269-9r7rqqqk 48 5 containing contain VBG cord-104269-9r7rqqqk 48 6 the the DT cord-104269-9r7rqqqk 48 7 entire entire JJ cord-104269-9r7rqqqk 48 8 coding code VBG cord-104269-9r7rqqqk 48 9 region region NN cord-104269-9r7rqqqk 48 10 of of IN cord-104269-9r7rqqqk 48 11 the the DT cord-104269-9r7rqqqk 48 12 wild wild JJ cord-104269-9r7rqqqk 48 13 - - HYPH cord-104269-9r7rqqqk 48 14 type type NN cord-104269-9r7rqqqk 48 15 human human NN cord-104269-9r7rqqqk 48 16 TR tr NN cord-104269-9r7rqqqk 48 17 was be VBD cord-104269-9r7rqqqk 48 18 cloned clone VBN cord-104269-9r7rqqqk 48 19 into into IN cord-104269-9r7rqqqk 48 20 the the DT cord-104269-9r7rqqqk 48 21 phagemid phagemid NN cord-104269-9r7rqqqk 48 22 pBluescript pBluescript NNP cord-104269-9r7rqqqk 48 23 SK SK NNP cord-104269-9r7rqqqk 48 24 ( ( -LRB- cord-104269-9r7rqqqk 48 25 Stratagene Stratagene NNP cord-104269-9r7rqqqk 48 26 , , , cord-104269-9r7rqqqk 48 27 La La NNP cord-104269-9r7rqqqk 48 28 JoLla JoLla NNP cord-104269-9r7rqqqk 48 29 , , , cord-104269-9r7rqqqk 48 30 CA CA NNP cord-104269-9r7rqqqk 48 31 ) ) -RRB- cord-104269-9r7rqqqk 48 32 . . . cord-104269-9r7rqqqk 49 1 Oligonucleotide oligonucleotide JJ cord-104269-9r7rqqqk 49 2 site site NN cord-104269-9r7rqqqk 49 3 - - HYPH cord-104269-9r7rqqqk 49 4 directed direct VBN cord-104269-9r7rqqqk 49 5 mutaganesis mutaganesis NN cord-104269-9r7rqqqk 49 6 was be VBD cord-104269-9r7rqqqk 49 7 used use VBN cord-104269-9r7rqqqk 49 8 to to TO cord-104269-9r7rqqqk 49 9 generate generate VB cord-104269-9r7rqqqk 49 10 the the DT cord-104269-9r7rqqqk 49 11 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 49 12 taft taft NNP cord-104269-9r7rqqqk 49 13 and and CC cord-104269-9r7rqqqk 49 14 transmembrane transmembrane NNP cord-104269-9r7rqqqk 49 15 domain domain NN cord-104269-9r7rqqqk 49 16 of of IN cord-104269-9r7rqqqk 49 17 Ii Ii NNP cord-104269-9r7rqqqk 49 18 ( ( -LRB- cord-104269-9r7rqqqk 49 19 Iic Iic NNP cord-104269-9r7rqqqk 49 20 - - HYPH cord-104269-9r7rqqqk 49 21 r r NNP cord-104269-9r7rqqqk 49 22 and and CC cord-104269-9r7rqqqk 49 23 IirM IirM NNP cord-104269-9r7rqqqk 49 24 ) ) -RRB- cord-104269-9r7rqqqk 50 1 chimeric chimeric JJ cord-104269-9r7rqqqk 50 2 constructs construct NNS cord-104269-9r7rqqqk 50 3 from from IN cord-104269-9r7rqqqk 50 4 a a DT cord-104269-9r7rqqqk 50 5 pBluescript pBluescript NNP cord-104269-9r7rqqqk 50 6 SK SK NNP cord-104269-9r7rqqqk 50 7 phagemid phagemid NN cord-104269-9r7rqqqk 50 8 template template NN cord-104269-9r7rqqqk 50 9 of of IN cord-104269-9r7rqqqk 50 10 the the DT cord-104269-9r7rqqqk 50 11 wild wild JJ cord-104269-9r7rqqqk 50 12 - - HYPH cord-104269-9r7rqqqk 50 13 type type NN cord-104269-9r7rqqqk 50 14 TR tr NN cord-104269-9r7rqqqk 50 15 by by IN cord-104269-9r7rqqqk 50 16 the the DT cord-104269-9r7rqqqk 50 17 method method NN cord-104269-9r7rqqqk 50 18 of of IN cord-104269-9r7rqqqk 50 19 Kunkel Kunkel NNP cord-104269-9r7rqqqk 50 20 ( ( -LRB- cord-104269-9r7rqqqk 50 21 1985 1985 CD cord-104269-9r7rqqqk 50 22 ) ) -RRB- cord-104269-9r7rqqqk 50 23 using use VBG cord-104269-9r7rqqqk 50 24 the the DT cord-104269-9r7rqqqk 50 25 Mnta Mnta NNP cord-104269-9r7rqqqk 50 26 - - HYPH cord-104269-9r7rqqqk 50 27 gene gene NN cord-104269-9r7rqqqk 50 28 mntagenesis mntagenesis NN cord-104269-9r7rqqqk 50 29 kit kit NN cord-104269-9r7rqqqk 50 30 ( ( -LRB- cord-104269-9r7rqqqk 50 31 Bio Bio NNP cord-104269-9r7rqqqk 50 32 - - HYPH cord-104269-9r7rqqqk 50 33 Rad Rad NNP cord-104269-9r7rqqqk 50 34 Labs Labs NNP cord-104269-9r7rqqqk 50 35 . . NNP cord-104269-9r7rqqqk 50 36 , , , cord-104269-9r7rqqqk 50 37 Richmond Richmond NNP cord-104269-9r7rqqqk 50 38 , , , cord-104269-9r7rqqqk 50 39 CA CA NNP cord-104269-9r7rqqqk 50 40 ) ) -RRB- cord-104269-9r7rqqqk 50 41 . . . cord-104269-9r7rqqqk 51 1 The the DT cord-104269-9r7rqqqk 51 2 same same JJ cord-104269-9r7rqqqk 51 3 method method NN cord-104269-9r7rqqqk 51 4 was be VBD cord-104269-9r7rqqqk 51 5 used use VBN cord-104269-9r7rqqqk 51 6 to to TO cord-104269-9r7rqqqk 51 7 generate generate VB cord-104269-9r7rqqqk 51 8 the the DT cord-104269-9r7rqqqk 51 9 Iic'r+zM Iic'r+zM NNP cord-104269-9r7rqqqk 51 10 chimeric chimeric JJ cord-104269-9r7rqqqk 51 11 construct construct NN cord-104269-9r7rqqqk 51 12 and and CC cord-104269-9r7rqqqk 51 13 mutant mutant JJ cord-104269-9r7rqqqk 51 14 IiCT iict NN cord-104269-9r7rqqqk 51 15 chimeric chimeric JJ cord-104269-9r7rqqqk 51 16 constructs construct NNS cord-104269-9r7rqqqk 51 17 from from IN cord-104269-9r7rqqqk 51 18 pBluescript pBluescript NNP cord-104269-9r7rqqqk 51 19 SK SK NNP cord-104269-9r7rqqqk 51 20 phagemid phagemid NN cord-104269-9r7rqqqk 51 21 templates template NNS cord-104269-9r7rqqqk 51 22 of of IN cord-104269-9r7rqqqk 51 23 the the DT cord-104269-9r7rqqqk 51 24 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 51 25 tail tail NN cord-104269-9r7rqqqk 51 26 of of IN cord-104269-9r7rqqqk 51 27 Ii Ii NNP cord-104269-9r7rqqqk 51 28 ( ( -LRB- cord-104269-9r7rqqqk 51 29 IicT IicT NNP cord-104269-9r7rqqqk 51 30 ) ) -RRB- cord-104269-9r7rqqqk 51 31 . . . cord-104269-9r7rqqqk 52 1 Oligonucleotides oligonucleotide NNS cord-104269-9r7rqqqk 52 2 were be VBD cord-104269-9r7rqqqk 52 3 prepared prepare VBN cord-104269-9r7rqqqk 52 4 on on IN cord-104269-9r7rqqqk 52 5 a a DT cord-104269-9r7rqqqk 52 6 model model NN cord-104269-9r7rqqqk 52 7 391 391 CD cord-104269-9r7rqqqk 52 8 PCR pcr NN cord-104269-9r7rqqqk 52 9 - - : cord-104269-9r7rqqqk 52 10 I I NNP cord-104269-9r7rqqqk 52 11 ~ ~ CD cord-104269-9r7rqqqk 52 12 TE TE NNP cord-104269-9r7rqqqk 52 13 DNA dna NN cord-104269-9r7rqqqk 52 14 synthesizer synthesizer NN cord-104269-9r7rqqqk 52 15 using use VBG cord-104269-9r7rqqqk 52 16 phosphoranddite phosphoranddite NNP cord-104269-9r7rqqqk 52 17 chemistry chemistry NN cord-104269-9r7rqqqk 52 18 and and CC cord-104269-9r7rqqqk 52 19 purified purify VBN cord-104269-9r7rqqqk 52 20 by by IN cord-104269-9r7rqqqk 52 21 polyacrylamide polyacrylamide NN cord-104269-9r7rqqqk 52 22 gel gel NN cord-104269-9r7rqqqk 52 23 electrophoresis electrophoresis NN cord-104269-9r7rqqqk 52 24 ( ( -LRB- cord-104269-9r7rqqqk 52 25 Appl Appl NNP cord-104269-9r7rqqqk 52 26 . . . cord-104269-9r7rqqqk 53 1 Biosystems Biosystems NNP cord-104269-9r7rqqqk 53 2 , , , cord-104269-9r7rqqqk 53 3 Inc. Inc. NNP cord-104269-9r7rqqqk 53 4 , , , cord-104269-9r7rqqqk 53 5 Foster Foster NNP cord-104269-9r7rqqqk 53 6 City City NNP cord-104269-9r7rqqqk 53 7 , , , cord-104269-9r7rqqqk 53 8 CA CA NNP cord-104269-9r7rqqqk 53 9 ) ) -RRB- cord-104269-9r7rqqqk 53 10 . . . cord-104269-9r7rqqqk 54 1 Mutants mutant NNS cord-104269-9r7rqqqk 54 2 were be VBD cord-104269-9r7rqqqk 54 3 selected select VBN cord-104269-9r7rqqqk 54 4 by by IN cord-104269-9r7rqqqk 54 5 restriction restriction NN cord-104269-9r7rqqqk 54 6 mapping mapping NN cord-104269-9r7rqqqk 54 7 or or CC cord-104269-9r7rqqqk 54 8 differential differential JJ cord-104269-9r7rqqqk 54 9 hybridization hybridization NN cord-104269-9r7rqqqk 54 10 , , , cord-104269-9r7rqqqk 54 11 and and CC cord-104269-9r7rqqqk 54 12 Cla Cla NNP cord-104269-9r7rqqqk 54 13 I -PRON- PRP cord-104269-9r7rqqqk 54 14 fragments fragment NNS cord-104269-9r7rqqqk 54 15 encoding encode VBG cord-104269-9r7rqqqk 54 16 the the DT cord-104269-9r7rqqqk 54 17 mutant mutant JJ cord-104269-9r7rqqqk 54 18 receptors receptor NNS cord-104269-9r7rqqqk 54 19 were be VBD cord-104269-9r7rqqqk 54 20 excised excise VBN cord-104269-9r7rqqqk 54 21 and and CC cord-104269-9r7rqqqk 54 22 cloned clone VBN cord-104269-9r7rqqqk 54 23 into into IN cord-104269-9r7rqqqk 54 24 the the DT cord-104269-9r7rqqqk 54 25 retroviral retroviral JJ cord-104269-9r7rqqqk 54 26 expression expression NN cord-104269-9r7rqqqk 54 27 vector vector NN cord-104269-9r7rqqqk 54 28 BH BH NNP cord-104269-9r7rqqqk 54 29 - - HYPH cord-104269-9r7rqqqk 54 30 RCAS RCAS NNP cord-104269-9r7rqqqk 55 1 ( ( -LRB- cord-104269-9r7rqqqk 55 2 Hughes Hughes NNP cord-104269-9r7rqqqk 55 3 et et NNP cord-104269-9r7rqqqk 55 4 al al NNP cord-104269-9r7rqqqk 55 5 . . NNP cord-104269-9r7rqqqk 55 6 , , , cord-104269-9r7rqqqk 55 7 1990 1990 CD cord-104269-9r7rqqqk 55 8 ) ) -RRB- cord-104269-9r7rqqqk 55 9 . . . cord-104269-9r7rqqqk 56 1 The the DT cord-104269-9r7rqqqk 56 2 mutations mutation NNS cord-104269-9r7rqqqk 56 3 were be VBD cord-104269-9r7rqqqk 56 4 verified verify VBN cord-104269-9r7rqqqk 56 5 by by IN cord-104269-9r7rqqqk 56 6 dideoxynucleotide dideoxynucleotide NN cord-104269-9r7rqqqk 56 7 sequencing sequencing NN cord-104269-9r7rqqqk 56 8 ( ( -LRB- cord-104269-9r7rqqqk 56 9 Sanger Sanger NNP cord-104269-9r7rqqqk 56 10 et et NNP cord-104269-9r7rqqqk 56 11 al al NNP cord-104269-9r7rqqqk 56 12 . . NNP cord-104269-9r7rqqqk 56 13 , , , cord-104269-9r7rqqqk 56 14 1977 1977 CD cord-104269-9r7rqqqk 56 15 ; ; : cord-104269-9r7rqqqk 56 16 Tabor Tabor NNP cord-104269-9r7rqqqk 56 17 and and CC cord-104269-9r7rqqqk 56 18 Richardson Richardson NNP cord-104269-9r7rqqqk 56 19 , , , cord-104269-9r7rqqqk 56 20 1987 1987 CD cord-104269-9r7rqqqk 56 21 ) ) -RRB- cord-104269-9r7rqqqk 56 22 of of IN cord-104269-9r7rqqqk 56 23 the the DT cord-104269-9r7rqqqk 56 24 BH BH NNP cord-104269-9r7rqqqk 56 25 - - HYPH cord-104269-9r7rqqqk 56 26 RCAS RCAS NNP cord-104269-9r7rqqqk 56 27 constructs construct NNS cord-104269-9r7rqqqk 56 28 using use VBG cord-104269-9r7rqqqk 56 29 the the DT cord-104269-9r7rqqqk 56 30 Sequenase Sequenase NNP cord-104269-9r7rqqqk 56 31 kit kit NN cord-104269-9r7rqqqk 56 32 ( ( -LRB- cord-104269-9r7rqqqk 56 33 US US NNP cord-104269-9r7rqqqk 56 34 Biochemical Biochemical NNP cord-104269-9r7rqqqk 56 35 Corp. Corp. NNP cord-104269-9r7rqqqk 56 36 , , , cord-104269-9r7rqqqk 56 37 Cleveland Cleveland NNP cord-104269-9r7rqqqk 56 38 , , , cord-104269-9r7rqqqk 56 39 OH OH NNP cord-104269-9r7rqqqk 56 40 ) ) -RRB- cord-104269-9r7rqqqk 56 41 according accord VBG cord-104269-9r7rqqqk 56 42 to to IN cord-104269-9r7rqqqk 56 43 the the DT cord-104269-9r7rqqqk 56 44 manufacturer manufacturer NN cord-104269-9r7rqqqk 56 45 's 's POS cord-104269-9r7rqqqk 56 46 directions direction NNS cord-104269-9r7rqqqk 56 47 . . . cord-104269-9r7rqqqk 57 1 Chicken chicken NN cord-104269-9r7rqqqk 57 2 embryo embryo NN cord-104269-9r7rqqqk 57 3 fibroblasts fibroblast NNS cord-104269-9r7rqqqk 57 4 ( ( -LRB- cord-104269-9r7rqqqk 57 5 CEF CEF NNP cord-104269-9r7rqqqk 57 6 ) ) -RRB- cord-104269-9r7rqqqk 57 7 were be VBD cord-104269-9r7rqqqk 57 8 prepared prepare VBN cord-104269-9r7rqqqk 57 9 from from IN cord-104269-9r7rqqqk 57 10 fertilized fertilize VBN cord-104269-9r7rqqqk 57 11 eggs egg NNS cord-104269-9r7rqqqk 57 12 ( ( -LRB- cord-104269-9r7rqqqk 57 13 SPAFAS SPAFAS NNP cord-104269-9r7rqqqk 57 14 , , , cord-104269-9r7rqqqk 57 15 Norwich Norwich NNP cord-104269-9r7rqqqk 57 16 , , , cord-104269-9r7rqqqk 57 17 CT CT NNP cord-104269-9r7rqqqk 57 18 ) ) -RRB- cord-104269-9r7rqqqk 57 19 and and CC cord-104269-9r7rqqqk 57 20 grown grow VBN cord-104269-9r7rqqqk 57 21 in in IN cord-104269-9r7rqqqk 57 22 DME DME NNP cord-104269-9r7rqqqk 57 23 supplemented supplement VBN cord-104269-9r7rqqqk 57 24 with with IN cord-104269-9r7rqqqk 57 25 1 1 CD cord-104269-9r7rqqqk 57 26 % % NN cord-104269-9r7rqqqk 57 27 ( ( -LRB- cord-104269-9r7rqqqk 57 28 vol vol NNP cord-104269-9r7rqqqk 57 29 / / SYM cord-104269-9r7rqqqk 57 30 vol vol NN cord-104269-9r7rqqqk 57 31 ) ) -RRB- cord-104269-9r7rqqqk 57 32 chicken chicken NN cord-104269-9r7rqqqk 57 33 serum serum NN cord-104269-9r7rqqqk 57 34 , , , cord-104269-9r7rqqqk 57 35 1 1 CD cord-104269-9r7rqqqk 57 36 % % NN cord-104269-9r7rqqqk 57 37 ( ( -LRB- cord-104269-9r7rqqqk 57 38 vol vol NNP cord-104269-9r7rqqqk 57 39 / / SYM cord-104269-9r7rqqqk 57 40 vol vol NN cord-104269-9r7rqqqk 57 41 ) ) -RRB- cord-104269-9r7rqqqk 58 1 defined define VBN cord-104269-9r7rqqqk 58 2 bovine bovine JJ cord-104269-9r7rqqqk 58 3 calf calf NN cord-104269-9r7rqqqk 58 4 serum serum NN cord-104269-9r7rqqqk 58 5 ( ( -LRB- cord-104269-9r7rqqqk 58 6 Hyclone Hyclone NNP cord-104269-9r7rqqqk 58 7 , , , cord-104269-9r7rqqqk 58 8 Logan Logan NNP cord-104269-9r7rqqqk 58 9 , , , cord-104269-9r7rqqqk 58 10 LIT LIT NNP cord-104269-9r7rqqqk 58 11 ) ) -RRB- cord-104269-9r7rqqqk 58 12 , , , cord-104269-9r7rqqqk 58 13 and and CC cord-104269-9r7rqqqk 58 14 2 2 CD cord-104269-9r7rqqqk 58 15 % % NN cord-104269-9r7rqqqk 58 16 ( ( -LRB- cord-104269-9r7rqqqk 58 17 vol vol NNP cord-104269-9r7rqqqk 58 18 / / SYM cord-104269-9r7rqqqk 58 19 vol vol NN cord-104269-9r7rqqqk 58 20 ) ) -RRB- cord-104269-9r7rqqqk 59 1 tryptose tryptose NNP cord-104269-9r7rqqqk 59 2 phosphate phosphate NN cord-104269-9r7rqqqk 59 3 broth broth NN cord-104269-9r7rqqqk 59 4 ( ( -LRB- cord-104269-9r7rqqqk 59 5 Difeo Difeo NNP cord-104269-9r7rqqqk 59 6 , , , cord-104269-9r7rqqqk 59 7 Detroit Detroit NNP cord-104269-9r7rqqqk 59 8 , , , cord-104269-9r7rqqqk 59 9 MI MI NNP cord-104269-9r7rqqqk 59 10 ) ) -RRB- cord-104269-9r7rqqqk 59 11 . . . cord-104269-9r7rqqqk 60 1 CEF CEF NNP cord-104269-9r7rqqqk 60 2 were be VBD cord-104269-9r7rqqqk 60 3 transfected transfecte VBN cord-104269-9r7rqqqk 60 4 with with IN cord-104269-9r7rqqqk 60 5 30/~g 30/~g CD cord-104269-9r7rqqqk 60 6 retroviral retroviral NN cord-104269-9r7rqqqk 60 7 construct construct VB cord-104269-9r7rqqqk 60 8 DNA dna NN cord-104269-9r7rqqqk 60 9 per per IN cord-104269-9r7rqqqk 60 10 10-cm 10-cm CD cord-104269-9r7rqqqk 60 11 tissue tissue NN cord-104269-9r7rqqqk 60 12 culture culture NN cord-104269-9r7rqqqk 60 13 plate plate NN cord-104269-9r7rqqqk 60 14 of of IN cord-104269-9r7rqqqk 60 15 40 40 CD cord-104269-9r7rqqqk 60 16 % % NN cord-104269-9r7rqqqk 60 17 confluent confluent JJ cord-104269-9r7rqqqk 60 18 cells cell NNS cord-104269-9r7rqqqk 60 19 using use VBG cord-104269-9r7rqqqk 60 20 the the DT cord-104269-9r7rqqqk 60 21 polybrene polybrene JJ cord-104269-9r7rqqqk 60 22 - - HYPH cord-104269-9r7rqqqk 60 23 dimethyl dimethyl NN cord-104269-9r7rqqqk 60 24 sulfoxide sulfoxide NN cord-104269-9r7rqqqk 60 25 method method NN cord-104269-9r7rqqqk 60 26 ( ( -LRB- cord-104269-9r7rqqqk 60 27 Kawai Kawai NNP cord-104269-9r7rqqqk 60 28 and and CC cord-104269-9r7rqqqk 60 29 Nishizawa Nishizawa NNP cord-104269-9r7rqqqk 60 30 , , , cord-104269-9r7rqqqk 60 31 1984 1984 CD cord-104269-9r7rqqqk 60 32 ) ) -RRB- cord-104269-9r7rqqqk 60 33 . . . cord-104269-9r7rqqqk 61 1 One one CD cord-104269-9r7rqqqk 61 2 to to TO cord-104269-9r7rqqqk 61 3 two two CD cord-104269-9r7rqqqk 61 4 weeks week NNS cord-104269-9r7rqqqk 61 5 after after IN cord-104269-9r7rqqqk 61 6 transfection transfection NN cord-104269-9r7rqqqk 61 7 with with IN cord-104269-9r7rqqqk 61 8 the the DT cord-104269-9r7rqqqk 61 9 BH BH NNP cord-104269-9r7rqqqk 61 10 - - HYPH cord-104269-9r7rqqqk 61 11 RCAS RCAS NNP cord-104269-9r7rqqqk 61 12 constructs construct VBZ cord-104269-9r7rqqqk 61 13 , , , cord-104269-9r7rqqqk 61 14 the the DT cord-104269-9r7rqqqk 61 15 CEF CEF NNP cord-104269-9r7rqqqk 61 16 stably stably RB cord-104269-9r7rqqqk 61 17 expressed express VBD cord-104269-9r7rqqqk 61 18 the the DT cord-104269-9r7rqqqk 61 19 wild wild JJ cord-104269-9r7rqqqk 61 20 - - HYPH cord-104269-9r7rqqqk 61 21 type type NN cord-104269-9r7rqqqk 61 22 TR tr NN cord-104269-9r7rqqqk 61 23 and and CC cord-104269-9r7rqqqk 61 24 chimeric chimeric JJ cord-104269-9r7rqqqk 61 25 Ii Ii NNP cord-104269-9r7rqqqk 61 26 - - HYPH cord-104269-9r7rqqqk 61 27 TR TR NNP cord-104269-9r7rqqqk 61 28 constructs construct NNS cord-104269-9r7rqqqk 61 29 as as IN cord-104269-9r7rqqqk 61 30 a a DT cord-104269-9r7rqqqk 61 31 result result NN cord-104269-9r7rqqqk 61 32 of of IN cord-104269-9r7rqqqk 61 33 infection infection NN cord-104269-9r7rqqqk 61 34 by by IN cord-104269-9r7rqqqk 61 35 recombinant recombinant JJ cord-104269-9r7rqqqk 61 36 virus virus NN cord-104269-9r7rqqqk 61 37 . . . cord-104269-9r7rqqqk 62 1 Surface surface NN cord-104269-9r7rqqqk 62 2 expression expression NN cord-104269-9r7rqqqk 62 3 levels level NNS cord-104269-9r7rqqqk 62 4 of of IN cord-104269-9r7rqqqk 62 5 the the DT cord-104269-9r7rqqqk 62 6 wild wild JJ cord-104269-9r7rqqqk 62 7 - - HYPH cord-104269-9r7rqqqk 62 8 type type NN cord-104269-9r7rqqqk 62 9 TR tr NN cord-104269-9r7rqqqk 62 10 and and CC cord-104269-9r7rqqqk 62 11 chimeric chimeric JJ cord-104269-9r7rqqqk 62 12 Ii Ii NNP cord-104269-9r7rqqqk 62 13 - - HYPH cord-104269-9r7rqqqk 62 14 TR TR NNP cord-104269-9r7rqqqk 62 15 constructs construct NNS cord-104269-9r7rqqqk 62 16 was be VBD cord-104269-9r7rqqqk 62 17 determined determine VBN cord-104269-9r7rqqqk 62 18 by by IN cord-104269-9r7rqqqk 62 19 measuring measure VBG cord-104269-9r7rqqqk 62 20 the the DT cord-104269-9r7rqqqk 62 21 binding binding NN cord-104269-9r7rqqqk 62 22 of of IN cord-104269-9r7rqqqk 62 23 125Ilabeled 125ilabeled CD cord-104269-9r7rqqqk 62 24 human human JJ cord-104269-9r7rqqqk 62 25 transferrin transferrin NN cord-104269-9r7rqqqk 62 26 ( ( -LRB- cord-104269-9r7rqqqk 62 27 Tf Tf NNP cord-104269-9r7rqqqk 62 28 ) ) -RRB- cord-104269-9r7rqqqk 62 29 at at IN cord-104269-9r7rqqqk 62 30 4 4 CD cord-104269-9r7rqqqk 62 31 ° ° NN cord-104269-9r7rqqqk 62 32 C c NN cord-104269-9r7rqqqk 62 33 . . . cord-104269-9r7rqqqk 63 1 Diferric Diferric NNP cord-104269-9r7rqqqk 63 2 human human JJ cord-104269-9r7rqqqk 64 1 " " `` cord-104269-9r7rqqqk 64 2 If if IN cord-104269-9r7rqqqk 64 3 ( ( -LRB- cord-104269-9r7rqqqk 64 4 Miles Miles NNP cord-104269-9r7rqqqk 64 5 Scientific Scientific NNP cord-104269-9r7rqqqk 64 6 , , , cord-104269-9r7rqqqk 64 7 Naperville Naperville NNP cord-104269-9r7rqqqk 64 8 , , , cord-104269-9r7rqqqk 64 9 IL IL NNP cord-104269-9r7rqqqk 64 10 ) ) -RRB- cord-104269-9r7rqqqk 64 11 was be VBD cord-104269-9r7rqqqk 64 12 labeled label VBN cord-104269-9r7rqqqk 64 13 with with IN cord-104269-9r7rqqqk 64 14 125I 125i CD cord-104269-9r7rqqqk 64 15 to to IN cord-104269-9r7rqqqk 64 16 a a DT cord-104269-9r7rqqqk 64 17 specific specific JJ cord-104269-9r7rqqqk 64 18 activity activity NN cord-104269-9r7rqqqk 64 19 of of IN cord-104269-9r7rqqqk 64 20 2 2 CD cord-104269-9r7rqqqk 64 21 - - HYPH cord-104269-9r7rqqqk 64 22 -4 -4 NN cord-104269-9r7rqqqk 64 23 # # NN cord-104269-9r7rqqqk 64 24 Ci/~tg Ci/~tg NNS cord-104269-9r7rqqqk 64 25 using use VBG cord-104269-9r7rqqqk 64 26 Enzymobeads Enzymobeads NNP cord-104269-9r7rqqqk 64 27 ( ( -LRB- cord-104269-9r7rqqqk 64 28 Bio Bio NNP cord-104269-9r7rqqqk 64 29 - - HYPH cord-104269-9r7rqqqk 64 30 Rad Rad NNP cord-104269-9r7rqqqk 64 31 Labs Labs NNP cord-104269-9r7rqqqk 64 32 . . . cord-104269-9r7rqqqk 64 33 ) ) -RRB- cord-104269-9r7rqqqk 65 1 according accord VBG cord-104269-9r7rqqqk 65 2 to to IN cord-104269-9r7rqqqk 65 3 the the DT cord-104269-9r7rqqqk 65 4 manufacturer manufacturer NN cord-104269-9r7rqqqk 65 5 's 's POS cord-104269-9r7rqqqk 65 6 directions direction NNS cord-104269-9r7rqqqk 65 7 . . . cord-104269-9r7rqqqk 66 1 CEF CEF NNP cord-104269-9r7rqqqk 66 2 were be VBD cord-104269-9r7rqqqk 66 3 plated plate VBN cord-104269-9r7rqqqk 66 4 in in IN cord-104269-9r7rqqqk 66 5 triplicate triplicate NN cord-104269-9r7rqqqk 66 6 at at IN cord-104269-9r7rqqqk 66 7 a a DT cord-104269-9r7rqqqk 66 8 density density NN cord-104269-9r7rqqqk 66 9 of of IN cord-104269-9r7rqqqk 66 10 7.5 7.5 CD cord-104269-9r7rqqqk 66 11 × × NN cord-104269-9r7rqqqk 66 12 104 104 CD cord-104269-9r7rqqqk 66 13 cells cell NNS cord-104269-9r7rqqqk 66 14 / / SYM cord-104269-9r7rqqqk 66 15 cm cm NN cord-104269-9r7rqqqk 66 16 2 2 CD cord-104269-9r7rqqqk 66 17 in in IN cord-104269-9r7rqqqk 66 18 24-well 24-well CD cord-104269-9r7rqqqk 66 19 tissue tissue NN cord-104269-9r7rqqqk 66 20 culture culture NN cord-104269-9r7rqqqk 66 21 plates plate NNS cord-104269-9r7rqqqk 66 22 24 24 CD cord-104269-9r7rqqqk 66 23 h h NN cord-104269-9r7rqqqk 66 24 before before IN cord-104269-9r7rqqqk 66 25 the the DT cord-104269-9r7rqqqk 66 26 binding bind VBG cord-104269-9r7rqqqk 66 27 assay assay NN cord-104269-9r7rqqqk 66 28 ( ( -LRB- cord-104269-9r7rqqqk 66 29 Costar Costar NNP cord-104269-9r7rqqqk 66 30 Corp. Corp. NNP cord-104269-9r7rqqqk 66 31 , , , cord-104269-9r7rqqqk 66 32 Cambridge Cambridge NNP cord-104269-9r7rqqqk 66 33 , , , cord-104269-9r7rqqqk 66 34 MA MA NNP cord-104269-9r7rqqqk 66 35 ) ) -RRB- cord-104269-9r7rqqqk 66 36 . . . cord-104269-9r7rqqqk 67 1 Cells cell NNS cord-104269-9r7rqqqk 67 2 were be VBD cord-104269-9r7rqqqk 67 3 incubated incubate VBN cord-104269-9r7rqqqk 67 4 in in IN cord-104269-9r7rqqqk 67 5 serum serum NN cord-104269-9r7rqqqk 67 6 - - HYPH cord-104269-9r7rqqqk 67 7 free free JJ cord-104269-9r7rqqqk 67 8 DME DME NNP cord-104269-9r7rqqqk 67 9 for for IN cord-104269-9r7rqqqk 67 10 1 1 CD cord-104269-9r7rqqqk 67 11 h h NN cord-104269-9r7rqqqk 67 12 at at IN cord-104269-9r7rqqqk 67 13 37 37 CD cord-104269-9r7rqqqk 67 14 ° ° NN cord-104269-9r7rqqqk 67 15 C c NN cord-104269-9r7rqqqk 67 16 , , , cord-104269-9r7rqqqk 67 17 and and CC cord-104269-9r7rqqqk 67 18 then then RB cord-104269-9r7rqqqk 67 19 washed wash VBD cord-104269-9r7rqqqk 67 20 once once RB cord-104269-9r7rqqqk 67 21 with with IN cord-104269-9r7rqqqk 67 22 ice ice NN cord-104269-9r7rqqqk 67 23 - - HYPH cord-104269-9r7rqqqk 67 24 cold cold JJ cord-104269-9r7rqqqk 67 25 0.15 0.15 CD cord-104269-9r7rqqqk 67 26 M M NNP cord-104269-9r7rqqqk 67 27 NaC1 NaC1 NNP cord-104269-9r7rqqqk 67 28 , , , cord-104269-9r7rqqqk 67 29 0.01 0.01 CD cord-104269-9r7rqqqk 67 30 M m NN cord-104269-9r7rqqqk 67 31 sodium sodium NN cord-104269-9r7rqqqk 67 32 phosphate phosphate NN cord-104269-9r7rqqqk 67 33 buffer buffer NN cord-104269-9r7rqqqk 67 34 ( ( -LRB- cord-104269-9r7rqqqk 67 35 pH pH NNP cord-104269-9r7rqqqk 67 36 7.4 7.4 CD cord-104269-9r7rqqqk 67 37 ) ) -RRB- cord-104269-9r7rqqqk 67 38 containing contain VBG cord-104269-9r7rqqqk 67 39 0.1 0.1 CD cord-104269-9r7rqqqk 67 40 % % NN cord-104269-9r7rqqqk 67 41 bovine bovine JJ cord-104269-9r7rqqqk 67 42 serum serum NN cord-104269-9r7rqqqk 67 43 albumin albumin NN cord-104269-9r7rqqqk 67 44 ( ( -LRB- cord-104269-9r7rqqqk 67 45 BSA BSA NNP cord-104269-9r7rqqqk 67 46 - - HYPH cord-104269-9r7rqqqk 67 47 PBS PBS NNP cord-104269-9r7rqqqk 67 48 ) ) -RRB- cord-104269-9r7rqqqk 67 49 . . . cord-104269-9r7rqqqk 68 1 t25Ilabeled t25Ilabeled NNP cord-104269-9r7rqqqk 68 2 Tf Tf NNP cord-104269-9r7rqqqk 68 3 ( ( -LRB- cord-104269-9r7rqqqk 68 4 4 4 CD cord-104269-9r7rqqqk 68 5 # # $ cord-104269-9r7rqqqk 68 6 g g NN cord-104269-9r7rqqqk 68 7 / / SYM cord-104269-9r7rqqqk 68 8 rnl rnl NNP cord-104269-9r7rqqqk 68 9 ) ) -RRB- cord-104269-9r7rqqqk 68 10 in in IN cord-104269-9r7rqqqk 68 11 0.15 0.15 CD cord-104269-9r7rqqqk 68 12 rnl rnl NNP cord-104269-9r7rqqqk 68 13 of of IN cord-104269-9r7rqqqk 68 14 BSA BSA NNP cord-104269-9r7rqqqk 68 15 - - HYPH cord-104269-9r7rqqqk 68 16 PBS PBS NNP cord-104269-9r7rqqqk 68 17 was be VBD cord-104269-9r7rqqqk 68 18 added add VBN cord-104269-9r7rqqqk 68 19 to to TO cord-104269-9r7rqqqk 68 20 triplicate triplicate VB cord-104269-9r7rqqqk 68 21 wells well NNS cord-104269-9r7rqqqk 68 22 and and CC cord-104269-9r7rqqqk 68 23 incubated incubate VBN cord-104269-9r7rqqqk 68 24 at at IN cord-104269-9r7rqqqk 68 25 4 4 CD cord-104269-9r7rqqqk 68 26 ° ° NN cord-104269-9r7rqqqk 68 27 C c NN cord-104269-9r7rqqqk 68 28 for for IN cord-104269-9r7rqqqk 68 29 60 60 CD cord-104269-9r7rqqqk 68 30 rain rain NN cord-104269-9r7rqqqk 68 31 . . . cord-104269-9r7rqqqk 69 1 Cells cell NNS cord-104269-9r7rqqqk 69 2 were be VBD cord-104269-9r7rqqqk 69 3 then then RB cord-104269-9r7rqqqk 69 4 washed wash VBN cord-104269-9r7rqqqk 69 5 three three CD cord-104269-9r7rqqqk 69 6 times time NNS cord-104269-9r7rqqqk 69 7 with with IN cord-104269-9r7rqqqk 69 8 0.5 0.5 CD cord-104269-9r7rqqqk 69 9 ml ml NNS cord-104269-9r7rqqqk 69 10 of of IN cord-104269-9r7rqqqk 69 11 ice ice NN cord-104269-9r7rqqqk 69 12 - - HYPH cord-104269-9r7rqqqk 69 13 cold cold JJ cord-104269-9r7rqqqk 69 14 BSA BSA NNP cord-104269-9r7rqqqk 69 15 - - HYPH cord-104269-9r7rqqqk 69 16 PBS PBS NNP cord-104269-9r7rqqqk 69 17 , , , cord-104269-9r7rqqqk 69 18 removed remove VBN cord-104269-9r7rqqqk 69 19 from from IN cord-104269-9r7rqqqk 69 20 the the DT cord-104269-9r7rqqqk 69 21 wells well NNS cord-104269-9r7rqqqk 69 22 with with IN cord-104269-9r7rqqqk 69 23 0.5 0.5 CD cord-104269-9r7rqqqk 69 24 ml ml NNS cord-104269-9r7rqqqk 69 25 of of IN cord-104269-9r7rqqqk 69 26 1 1 CD cord-104269-9r7rqqqk 69 27 M M NNP cord-104269-9r7rqqqk 69 28 NaOH NaOH NNP cord-104269-9r7rqqqk 69 29 , , , cord-104269-9r7rqqqk 69 30 and and CC cord-104269-9r7rqqqk 69 31 the the DT cord-104269-9r7rqqqk 69 32 radioactivity radioactivity NN cord-104269-9r7rqqqk 69 33 was be VBD cord-104269-9r7rqqqk 69 34 counted count VBN cord-104269-9r7rqqqk 69 35 in in IN cord-104269-9r7rqqqk 69 36 a a DT cord-104269-9r7rqqqk 69 37 gamma gamma NN cord-104269-9r7rqqqk 69 38 counter counter NN cord-104269-9r7rqqqk 69 39 . . . cord-104269-9r7rqqqk 70 1 The the DT cord-104269-9r7rqqqk 70 2 apparent apparent JJ cord-104269-9r7rqqqk 70 3 internalization internalization NN cord-104269-9r7rqqqk 70 4 efficiencies efficiency NNS cord-104269-9r7rqqqk 70 5 of of IN cord-104269-9r7rqqqk 70 6 the the DT cord-104269-9r7rqqqk 70 7 wild wild JJ cord-104269-9r7rqqqk 70 8 - - HYPH cord-104269-9r7rqqqk 70 9 type type NN cord-104269-9r7rqqqk 70 10 TR tr NN cord-104269-9r7rqqqk 70 11 and and CC cord-104269-9r7rqqqk 70 12 chimeric chimeric JJ cord-104269-9r7rqqqk 70 13 Ii Ii NNP cord-104269-9r7rqqqk 70 14 - - HYPH cord-104269-9r7rqqqk 70 15 TR TR NNP cord-104269-9r7rqqqk 70 16 constructs construct NNS cord-104269-9r7rqqqk 70 17 were be VBD cord-104269-9r7rqqqk 70 18 estimated estimate VBN cord-104269-9r7rqqqk 70 19 from from IN cord-104269-9r7rqqqk 70 20 measurements measurement NNS cord-104269-9r7rqqqk 70 21 of of IN cord-104269-9r7rqqqk 70 22 the the DT cord-104269-9r7rqqqk 70 23 steady steady JJ cord-104269-9r7rqqqk 70 24 - - HYPH cord-104269-9r7rqqqk 70 25 state state NN cord-104269-9r7rqqqk 70 26 distribution distribution NN cord-104269-9r7rqqqk 70 27 of of IN cord-104269-9r7rqqqk 70 28 receptors receptor NNS cord-104269-9r7rqqqk 70 29 at at IN cord-104269-9r7rqqqk 70 30 37 37 CD cord-104269-9r7rqqqk 70 31 ° ° NN cord-104269-9r7rqqqk 70 32 C C NNP cord-104269-9r7rqqqk 70 33 ( ( -LRB- cord-104269-9r7rqqqk 70 34 Tanner Tanner NNP cord-104269-9r7rqqqk 70 35 and and CC cord-104269-9r7rqqqk 70 36 Leinhard Leinhard NNP cord-104269-9r7rqqqk 70 37 , , , cord-104269-9r7rqqqk 70 38 1987 1987 CD cord-104269-9r7rqqqk 70 39 ) ) -RRB- cord-104269-9r7rqqqk 70 40 . . . cord-104269-9r7rqqqk 71 1 CEF CEF NNP cord-104269-9r7rqqqk 71 2 were be VBD cord-104269-9r7rqqqk 71 3 plated plate VBN cord-104269-9r7rqqqk 71 4 in in IN cord-104269-9r7rqqqk 71 5 triplicate triplicate JJ cord-104269-9r7rqqqk 71 6 wells well NNS cord-104269-9r7rqqqk 71 7 as as IN cord-104269-9r7rqqqk 71 8 described describe VBN cord-104269-9r7rqqqk 71 9 for for IN cord-104269-9r7rqqqk 71 10 the the DT cord-104269-9r7rqqqk 71 11 binding bind VBG cord-104269-9r7rqqqk 71 12 studies study NNS cord-104269-9r7rqqqk 71 13 . . . cord-104269-9r7rqqqk 72 1 The the DT cord-104269-9r7rqqqk 72 2 cells cell NNS cord-104269-9r7rqqqk 72 3 were be VBD cord-104269-9r7rqqqk 72 4 first first RB cord-104269-9r7rqqqk 72 5 incubated incubate VBN cord-104269-9r7rqqqk 72 6 in in IN cord-104269-9r7rqqqk 72 7 serum serum NN cord-104269-9r7rqqqk 72 8 - - HYPH cord-104269-9r7rqqqk 72 9 free free JJ cord-104269-9r7rqqqk 72 10 DME DME NNP cord-104269-9r7rqqqk 72 11 , , , cord-104269-9r7rqqqk 72 12 and and CC cord-104269-9r7rqqqk 72 13 then then RB cord-104269-9r7rqqqk 72 14 incubated incubate VBD cord-104269-9r7rqqqk 72 15 with with IN cord-104269-9r7rqqqk 72 16 4 4 CD cord-104269-9r7rqqqk 72 17 / / SYM cord-104269-9r7rqqqk 72 18 tg tg NNP cord-104269-9r7rqqqk 72 19 / / SYM cord-104269-9r7rqqqk 72 20 ml ml NNP cord-104269-9r7rqqqk 72 21 t2Sllabeled t2sllabele VBN cord-104269-9r7rqqqk 72 22 " " `` cord-104269-9r7rqqqk 72 23 If if IN cord-104269-9r7rqqqk 72 24 in in IN cord-104269-9r7rqqqk 72 25 BSA BSA NNP cord-104269-9r7rqqqk 72 26 - - HYPH cord-104269-9r7rqqqk 72 27 PBS PBS NNP cord-104269-9r7rqqqk 72 28 for for IN cord-104269-9r7rqqqk 72 29 I -PRON- PRP cord-104269-9r7rqqqk 73 1 h h NN cord-104269-9r7rqqqk 73 2 at at IN cord-104269-9r7rqqqk 73 3 37 37 CD cord-104269-9r7rqqqk 73 4 ° ° NN cord-104269-9r7rqqqk 73 5 C c NN cord-104269-9r7rqqqk 73 6 . . . cord-104269-9r7rqqqk 74 1 The the DT cord-104269-9r7rqqqk 74 2 labeling labeling NN cord-104269-9r7rqqqk 74 3 medium medium NN cord-104269-9r7rqqqk 74 4 was be VBD cord-104269-9r7rqqqk 74 5 removed remove VBN cord-104269-9r7rqqqk 74 6 , , , cord-104269-9r7rqqqk 74 7 and and CC cord-104269-9r7rqqqk 74 8 the the DT cord-104269-9r7rqqqk 74 9 cells cell NNS cord-104269-9r7rqqqk 74 10 were be VBD cord-104269-9r7rqqqk 74 11 washed wash VBN cord-104269-9r7rqqqk 74 12 three three CD cord-104269-9r7rqqqk 74 13 times time NNS cord-104269-9r7rqqqk 74 14 with with IN cord-104269-9r7rqqqk 74 15 1 1 CD cord-104269-9r7rqqqk 74 16 ml ml NN cord-104269-9r7rqqqk 74 17 of of IN cord-104269-9r7rqqqk 74 18 ice ice NN cord-104269-9r7rqqqk 74 19 - - HYPH cord-104269-9r7rqqqk 74 20 cold cold JJ cord-104269-9r7rqqqk 74 21 BSA BSA NNP cord-104269-9r7rqqqk 74 22 - - HYPH cord-104269-9r7rqqqk 74 23 PBS PBS NNP cord-104269-9r7rqqqk 74 24 , , , cord-104269-9r7rqqqk 74 25 and and CC cord-104269-9r7rqqqk 74 26 then then RB cord-104269-9r7rqqqk 74 27 incubated incubate VBN cord-104269-9r7rqqqk 74 28 twice twice RB cord-104269-9r7rqqqk 74 29 for for IN cord-104269-9r7rqqqk 74 30 3 3 CD cord-104269-9r7rqqqk 74 31 min min NN cord-104269-9r7rqqqk 74 32 with with IN cord-104269-9r7rqqqk 75 1 0.5 0.5 CD cord-104269-9r7rqqqk 75 2 m m NN cord-104269-9r7rqqqk 75 3 ] ] -RRB- cord-104269-9r7rqqqk 75 4 0.2 0.2 CD cord-104269-9r7rqqqk 75 5 M m NN cord-104269-9r7rqqqk 76 1 acetic acetic JJ cord-104269-9r7rqqqk 76 2 acid-0.5 acid-0.5 NNP cord-104269-9r7rqqqk 76 3 M M NNP cord-104269-9r7rqqqk 76 4 NaCI naci NN cord-104269-9r7rqqqk 77 1 ( ( -LRB- cord-104269-9r7rqqqk 77 2 pH pH NNP cord-104269-9r7rqqqk 77 3 2.4 2.4 CD cord-104269-9r7rqqqk 77 4 ) ) -RRB- cord-104269-9r7rqqqk 77 5 to to TO cord-104269-9r7rqqqk 77 6 remove remove VB cord-104269-9r7rqqqk 77 7 surface surface NN cord-104269-9r7rqqqk 77 8 - - HYPH cord-104269-9r7rqqqk 77 9 bound bind VBN cord-104269-9r7rqqqk 77 10 1251 1251 CD cord-104269-9r7rqqqk 77 11 - - HYPH cord-104269-9r7rqqqk 77 12 1abeled 1abeled CD cord-104269-9r7rqqqk 77 13 Tf Tf NNP cord-104269-9r7rqqqk 77 14 ( ( -LRB- cord-104269-9r7rqqqk 77 15 Hopkins Hopkins NNP cord-104269-9r7rqqqk 77 16 and and CC cord-104269-9r7rqqqk 77 17 Trowbridge Trowbridge NNP cord-104269-9r7rqqqk 77 18 , , , cord-104269-9r7rqqqk 77 19 1983 1983 CD cord-104269-9r7rqqqk 77 20 ) ) -RRB- cord-104269-9r7rqqqk 77 21 , , , cord-104269-9r7rqqqk 77 22 and and CC cord-104269-9r7rqqqk 77 23 removed remove VBN cord-104269-9r7rqqqk 77 24 from from IN cord-104269-9r7rqqqk 77 25 the the DT cord-104269-9r7rqqqk 77 26 wells well NNS cord-104269-9r7rqqqk 77 27 with with IN cord-104269-9r7rqqqk 77 28 I I NNP cord-104269-9r7rqqqk 77 29 M M NNP cord-104269-9r7rqqqk 77 30 NaOH NaOH NNP cord-104269-9r7rqqqk 77 31 . . . cord-104269-9r7rqqqk 78 1 Radioactivity radioactivity NN cord-104269-9r7rqqqk 78 2 in in IN cord-104269-9r7rqqqk 78 3 the the DT cord-104269-9r7rqqqk 78 4 acid acid NN cord-104269-9r7rqqqk 78 5 wash wash NN cord-104269-9r7rqqqk 78 6 and and CC cord-104269-9r7rqqqk 78 7 in in IN cord-104269-9r7rqqqk 78 8 the the DT cord-104269-9r7rqqqk 78 9 cell cell NN cord-104269-9r7rqqqk 78 10 lysete lysete NN cord-104269-9r7rqqqk 78 11 was be VBD cord-104269-9r7rqqqk 78 12 determined determine VBN cord-104269-9r7rqqqk 78 13 . . . cord-104269-9r7rqqqk 79 1 More more RBR cord-104269-9r7rqqqk 79 2 prolonged prolong VBN cord-104269-9r7rqqqk 79 3 incubation incubation NN cord-104269-9r7rqqqk 79 4 with with IN cord-104269-9r7rqqqk 79 5 the the DT cord-104269-9r7rqqqk 79 6 acid acid NN cord-104269-9r7rqqqk 79 7 wash wash NN cord-104269-9r7rqqqk 79 8 did do VBD cord-104269-9r7rqqqk 79 9 not not RB cord-104269-9r7rqqqk 79 10 change change VB cord-104269-9r7rqqqk 79 11 the the DT cord-104269-9r7rqqqk 79 12 amount amount NN cord-104269-9r7rqqqk 79 13 of of IN cord-104269-9r7rqqqk 79 14 t251 t251 NN cord-104269-9r7rqqqk 79 15 released release VBN cord-104269-9r7rqqqk 79 16 . . . cord-104269-9r7rqqqk 80 1 At at IN cord-104269-9r7rqqqk 80 2 steady steady JJ cord-104269-9r7rqqqk 80 3 state state NN cord-104269-9r7rqqqk 80 4 , , , cord-104269-9r7rqqqk 80 5 the the DT cord-104269-9r7rqqqk 80 6 rate rate NN cord-104269-9r7rqqqk 80 7 of of IN cord-104269-9r7rqqqk 80 8 internalization internalization NN cord-104269-9r7rqqqk 80 9 , , , cord-104269-9r7rqqqk 80 10 kint kint NNP cord-104269-9r7rqqqk 80 11 , , , cord-104269-9r7rqqqk 80 12 of of IN cord-104269-9r7rqqqk 80 13 cell cell NN cord-104269-9r7rqqqk 80 14 surface surface NN cord-104269-9r7rqqqk 80 15 Tf Tf NNP cord-104269-9r7rqqqk 80 16 . . . cord-104269-9r7rqqqk 80 17 TR TR NNP cord-104269-9r7rqqqk 80 18 complexes complex NNS cord-104269-9r7rqqqk 80 19 , , , cord-104269-9r7rqqqk 80 20 [ [ -LRB- cord-104269-9r7rqqqk 80 21 TR TR NNP cord-104269-9r7rqqqk 80 22 ] ] -RRB- cord-104269-9r7rqqqk 80 23 .... .... NFP cord-104269-9r7rqqqk 80 24 equals equal VBZ cord-104269-9r7rqqqk 80 25 the the DT cord-104269-9r7rqqqk 80 26 rate rate NN cord-104269-9r7rqqqk 80 27 of of IN cord-104269-9r7rqqqk 80 28 externalization externalization NN cord-104269-9r7rqqqk 80 29 , , , cord-104269-9r7rqqqk 80 30 kext kext NN cord-104269-9r7rqqqk 80 31 , , , cord-104269-9r7rqqqk 80 32 of of IN cord-104269-9r7rqqqk 80 33 the the DT cord-104269-9r7rqqqk 80 34 internal internal JJ cord-104269-9r7rqqqk 80 35 pool pool NN cord-104269-9r7rqqqk 80 36 of of IN cord-104269-9r7rqqqk 80 37 apoTf apotf RB cord-104269-9r7rqqqk 80 38 . . . cord-104269-9r7rqqqk 81 1 TR TR NNP cord-104269-9r7rqqqk 81 2 complexes complex NNS cord-104269-9r7rqqqk 81 3 , , , cord-104269-9r7rqqqk 81 4 [ [ -LRB- cord-104269-9r7rqqqk 81 5 TR]~ TR]~ NNP cord-104269-9r7rqqqk 81 6 ; ; : cord-104269-9r7rqqqk 81 7 i.e. i.e. FW cord-104269-9r7rqqqk 81 8 , , , cord-104269-9r7rqqqk 81 9 kint'[TR]sur kint'[tr]sur ADD cord-104269-9r7rqqqk 81 10 = = SYM cord-104269-9r7rqqqk 81 11 kext'~I'R]int kext'~I'R]int NNP cord-104269-9r7rqqqk 81 12 , , , cord-104269-9r7rqqqk 82 1 assuming assume VBG cord-104269-9r7rqqqk 82 2 an an DT cord-104269-9r7rqqqk 82 3 insignificant insignificant JJ cord-104269-9r7rqqqk 82 4 rate rate NN cord-104269-9r7rqqqk 82 5 of of IN cord-104269-9r7rqqqk 82 6 degradation degradation NN cord-104269-9r7rqqqk 82 7 of of IN cord-104269-9r7rqqqk 82 8 internalized internalize VBN cord-104269-9r7rqqqk 82 9 receptors receptor NNS cord-104269-9r7rqqqk 82 10 during during IN cord-104269-9r7rqqqk 82 11 the the DT cord-104269-9r7rqqqk 82 12 time time NN cord-104269-9r7rqqqk 82 13 required require VBN cord-104269-9r7rqqqk 82 14 to to TO cord-104269-9r7rqqqk 82 15 achieve achieve VB cord-104269-9r7rqqqk 82 16 steady steady JJ cord-104269-9r7rqqqk 82 17 - - HYPH cord-104269-9r7rqqqk 82 18 state state NN cord-104269-9r7rqqqk 82 19 . . . cord-104269-9r7rqqqk 83 1 The the DT cord-104269-9r7rqqqk 83 2 values value NNS cord-104269-9r7rqqqk 83 3 of of IN cord-104269-9r7rqqqk 83 4 [ [ -LRB- cord-104269-9r7rqqqk 83 5 ' ' '' cord-104269-9r7rqqqk 83 6 I'R]~ I'R]~ VBN cord-104269-9r7rqqqk 83 7 and and CC cord-104269-9r7rqqqk 83 8 [ [ -LRB- cord-104269-9r7rqqqk 83 9 TR]mt tr]mt PRP cord-104269-9r7rqqqk 83 10 can can MD cord-104269-9r7rqqqk 83 11 be be VB cord-104269-9r7rqqqk 83 12 obtained obtain VBN cord-104269-9r7rqqqk 83 13 from from IN cord-104269-9r7rqqqk 83 14 steady steady JJ cord-104269-9r7rqqqk 83 15 - - HYPH cord-104269-9r7rqqqk 83 16 state state NN cord-104269-9r7rqqqk 83 17 binding binding NN cord-104269-9r7rqqqk 83 18 of of IN cord-104269-9r7rqqqk 83 19 TR tr NN cord-104269-9r7rqqqk 83 20 under under IN cord-104269-9r7rqqqk 83 21 saturating saturate VBG cord-104269-9r7rqqqk 83 22 conditions condition NNS cord-104269-9r7rqqqk 83 23 at at IN cord-104269-9r7rqqqk 83 24 37 37 CD cord-104269-9r7rqqqk 83 25 ° ° NN cord-104269-9r7rqqqk 83 26 C c NN cord-104269-9r7rqqqk 83 27 . . . cord-104269-9r7rqqqk 84 1 As as IN cord-104269-9r7rqqqk 84 2 ke ke NNP cord-104269-9r7rqqqk 84 3 ~ ~ NNP cord-104269-9r7rqqqk 84 4 t t NNP cord-104269-9r7rqqqk 84 5 of of IN cord-104269-9r7rqqqk 84 6 apoTf apotf RB cord-104269-9r7rqqqk 84 7 . . . cord-104269-9r7rqqqk 85 1 TR TR NNP cord-104269-9r7rqqqk 85 2 complexes complex NNS cord-104269-9r7rqqqk 85 3 is be VBZ cord-104269-9r7rqqqk 85 4 independent independent JJ cord-104269-9r7rqqqk 85 5 of of IN cord-104269-9r7rqqqk 85 6 signals signal NNS cord-104269-9r7rqqqk 85 7 in in IN cord-104269-9r7rqqqk 85 8 the the DT cord-104269-9r7rqqqk 85 9 TR tr NN cord-104269-9r7rqqqk 85 10 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 85 11 domain domain NN cord-104269-9r7rqqqk 85 12 , , , cord-104269-9r7rqqqk 85 13 k k NNP cord-104269-9r7rqqqk 85 14 ~ ~ NNP cord-104269-9r7rqqqk 85 15 xt xt NNP cord-104269-9r7rqqqk 85 16 values value NNS cord-104269-9r7rqqqk 85 17 of of IN cord-104269-9r7rqqqk 85 18 mutant mutant JJ cord-104269-9r7rqqqk 85 19 and and CC cord-104269-9r7rqqqk 85 20 wild wild JJ cord-104269-9r7rqqqk 85 21 - - HYPH cord-104269-9r7rqqqk 85 22 type type NN cord-104269-9r7rqqqk 85 23 receptors receptor NNS cord-104269-9r7rqqqk 85 24 are be VBP cord-104269-9r7rqqqk 85 25 identical identical JJ cord-104269-9r7rqqqk 85 26 so so IN cord-104269-9r7rqqqk 85 27 that that IN cord-104269-9r7rqqqk 85 28 their -PRON- PRP$ cord-104269-9r7rqqqk 85 29 k~ k~ CD cord-104269-9r7rqqqk 85 30 values value NNS cord-104269-9r7rqqqk 85 31 are be VBP cord-104269-9r7rqqqk 85 32 proportional proportional JJ cord-104269-9r7rqqqk 85 33 to to IN cord-104269-9r7rqqqk 85 34 their -PRON- PRP$ cord-104269-9r7rqqqk 85 35 steady steady JJ cord-104269-9r7rqqqk 85 36 state state NN cord-104269-9r7rqqqk 85 37 distribution distribution NN cord-104269-9r7rqqqk 85 38 , , , cord-104269-9r7rqqqk 85 39 [ [ -LRB- cord-104269-9r7rqqqk 85 40 TR tr NN cord-104269-9r7rqqqk 85 41 ] ] -RRB- cord-104269-9r7rqqqk 85 42 _SP cord-104269-9r7rqqqk 86 1 The the DT cord-104269-9r7rqqqk 86 2 internalization internalization NN cord-104269-9r7rqqqk 86 3 efficiencies efficiency NNS cord-104269-9r7rqqqk 86 4 of of IN cord-104269-9r7rqqqk 86 5 wild wild JJ cord-104269-9r7rqqqk 86 6 - - HYPH cord-104269-9r7rqqqk 86 7 type type NN cord-104269-9r7rqqqk 86 8 TR tr NN cord-104269-9r7rqqqk 86 9 and and CC cord-104269-9r7rqqqk 86 10 Ii Ii NNP cord-104269-9r7rqqqk 86 11 - - HYPH cord-104269-9r7rqqqk 86 12 TR TR NNP cord-104269-9r7rqqqk 86 13 chimeras chimera NNS cord-104269-9r7rqqqk 86 14 were be VBD cord-104269-9r7rqqqk 86 15 also also RB cord-104269-9r7rqqqk 86 16 determined determine VBN cord-104269-9r7rqqqk 86 17 by by IN cord-104269-9r7rqqqk 86 18 measuring measure VBG cord-104269-9r7rqqqk 86 19 their -PRON- PRP$ cord-104269-9r7rqqqk 86 20 ability ability NN cord-104269-9r7rqqqk 86 21 to to TO cord-104269-9r7rqqqk 86 22 mediate mediate VB cord-104269-9r7rqqqk 86 23 iron iron NN cord-104269-9r7rqqqk 86 24 uptake uptake NN cord-104269-9r7rqqqk 86 25 . . . cord-104269-9r7rqqqk 87 1 Human human JJ cord-104269-9r7rqqqk 87 2 apo apo NNP cord-104269-9r7rqqqk 87 3 - - HYPH cord-104269-9r7rqqqk 87 4 Tf Tf NNP cord-104269-9r7rqqqk 87 5 was be VBD cord-104269-9r7rqqqk 87 6 labeled label VBN cord-104269-9r7rqqqk 87 7 with with IN cord-104269-9r7rqqqk 87 8 59Fe 59Fe NNP cord-104269-9r7rqqqk 87 9 ( ( -LRB- cord-104269-9r7rqqqk 87 10 FeCI3 FeCI3 NNP cord-104269-9r7rqqqk 87 11 ; ; : cord-104269-9r7rqqqk 87 12 Amersham Amersham NNP cord-104269-9r7rqqqk 87 13 Corp. Corp. NNP cord-104269-9r7rqqqk 87 14 , , , cord-104269-9r7rqqqk 87 15 Arlington Arlington NNP cord-104269-9r7rqqqk 87 16 Heights Heights NNP cord-104269-9r7rqqqk 87 17 , , , cord-104269-9r7rqqqk 87 18 IL IL NNP cord-104269-9r7rqqqk 87 19 ) ) -RRB- cord-104269-9r7rqqqk 87 20 to to IN cord-104269-9r7rqqqk 87 21 a a DT cord-104269-9r7rqqqk 87 22 specific specific JJ cord-104269-9r7rqqqk 87 23 activity activity NN cord-104269-9r7rqqqk 87 24 of of IN cord-104269-9r7rqqqk 87 25 5 5 CD cord-104269-9r7rqqqk 87 26 - - SYM cord-104269-9r7rqqqk 87 27 10 10 CD cord-104269-9r7rqqqk 87 28 # # $ cord-104269-9r7rqqqk 87 29 Ci ci NN cord-104269-9r7rqqqk 87 30 / / SYM cord-104269-9r7rqqqk 87 31 ttg ttg NNP cord-104269-9r7rqqqk 87 32 using use VBG cord-104269-9r7rqqqk 87 33 ultrilotriacetate ultrilotriacetate NNP cord-104269-9r7rqqqk 87 34 ( ( -LRB- cord-104269-9r7rqqqk 87 35 Bates Bates NNP cord-104269-9r7rqqqk 87 36 and and CC cord-104269-9r7rqqqk 87 37 Schlabach Schlabach NNP cord-104269-9r7rqqqk 87 38 , , , cord-104269-9r7rqqqk 87 39 1973 1973 CD cord-104269-9r7rqqqk 87 40 ) ) -RRB- cord-104269-9r7rqqqk 87 41 . . . cord-104269-9r7rqqqk 88 1 Cells cell NNS cord-104269-9r7rqqqk 88 2 were be VBD cord-104269-9r7rqqqk 88 3 plated plate VBN cord-104269-9r7rqqqk 88 4 in in IN cord-104269-9r7rqqqk 88 5 triplicate triplicate JJ cord-104269-9r7rqqqk 88 6 wells well NNS cord-104269-9r7rqqqk 88 7 as as IN cord-104269-9r7rqqqk 88 8 described describe VBN cord-104269-9r7rqqqk 88 9 for for IN cord-104269-9r7rqqqk 88 10 the the DT cord-104269-9r7rqqqk 88 11 binding bind VBG cord-104269-9r7rqqqk 88 12 studies study NNS cord-104269-9r7rqqqk 88 13 . . . cord-104269-9r7rqqqk 89 1 The the DT cord-104269-9r7rqqqk 89 2 following following JJ cord-104269-9r7rqqqk 89 3 day day NN cord-104269-9r7rqqqk 89 4 , , , cord-104269-9r7rqqqk 89 5 cells cell NNS cord-104269-9r7rqqqk 89 6 were be VBD cord-104269-9r7rqqqk 89 7 washed wash VBN cord-104269-9r7rqqqk 89 8 twice twice RB cord-104269-9r7rqqqk 89 9 in in IN cord-104269-9r7rqqqk 89 10 prewarmed prewarmed JJ cord-104269-9r7rqqqk 89 11 ( ( -LRB- cord-104269-9r7rqqqk 89 12 37 37 CD cord-104269-9r7rqqqk 89 13 ° ° NN cord-104269-9r7rqqqk 89 14 C c NN cord-104269-9r7rqqqk 89 15 ) ) -RRB- cord-104269-9r7rqqqk 89 16 serum serum NN cord-104269-9r7rqqqk 89 17 - - HYPH cord-104269-9r7rqqqk 89 18 free free JJ cord-104269-9r7rqqqk 89 19 DME DME NNP cord-104269-9r7rqqqk 89 20 , , , cord-104269-9r7rqqqk 89 21 and and CC cord-104269-9r7rqqqk 89 22 then then RB cord-104269-9r7rqqqk 89 23 incubated incubate VBN cord-104269-9r7rqqqk 89 24 in in IN cord-104269-9r7rqqqk 89 25 DME DME NNP cord-104269-9r7rqqqk 89 26 containing contain VBG cord-104269-9r7rqqqk 89 27 0.1 0.1 CD cord-104269-9r7rqqqk 89 28 % % NN cord-104269-9r7rqqqk 89 29 BSA BSA NNP cord-104269-9r7rqqqk 89 30 and and CC cord-104269-9r7rqqqk 89 31 4 4 CD cord-104269-9r7rqqqk 89 32 t t NN cord-104269-9r7rqqqk 89 33 ~ ~ CD cord-104269-9r7rqqqk 89 34 g g NN cord-104269-9r7rqqqk 89 35 / / SYM cord-104269-9r7rqqqk 89 36 m159Fe m159fe NN cord-104269-9r7rqqqk 89 37 - - HYPH cord-104269-9r7rqqqk 89 38 Tf Tf NNP cord-104269-9r7rqqqk 89 39 at at IN cord-104269-9r7rqqqk 89 40 37 37 CD cord-104269-9r7rqqqk 89 41 ° ° NN cord-104269-9r7rqqqk 89 42 C c NN cord-104269-9r7rqqqk 89 43 for for IN cord-104269-9r7rqqqk 89 44 0 0 CD cord-104269-9r7rqqqk 89 45 , , , cord-104269-9r7rqqqk 89 46 1 1 CD cord-104269-9r7rqqqk 89 47 , , , cord-104269-9r7rqqqk 89 48 2 2 CD cord-104269-9r7rqqqk 89 49 , , , cord-104269-9r7rqqqk 89 50 3 3 CD cord-104269-9r7rqqqk 89 51 , , , cord-104269-9r7rqqqk 89 52 and and CC cord-104269-9r7rqqqk 89 53 4 4 CD cord-104269-9r7rqqqk 89 54 h. h. NN cord-104269-9r7rqqqk 90 1 At at IN cord-104269-9r7rqqqk 90 2 the the DT cord-104269-9r7rqqqk 90 3 indicated indicate VBN cord-104269-9r7rqqqk 90 4 times time NNS cord-104269-9r7rqqqk 90 5 , , , cord-104269-9r7rqqqk 90 6 the the DT cord-104269-9r7rqqqk 90 7 medium medium NN cord-104269-9r7rqqqk 90 8 was be VBD cord-104269-9r7rqqqk 90 9 removed remove VBN cord-104269-9r7rqqqk 90 10 , , , cord-104269-9r7rqqqk 90 11 and and CC cord-104269-9r7rqqqk 90 12 cells cell NNS cord-104269-9r7rqqqk 90 13 were be VBD cord-104269-9r7rqqqk 90 14 washed wash VBN cord-104269-9r7rqqqk 90 15 three three CD cord-104269-9r7rqqqk 90 16 times time NNS cord-104269-9r7rqqqk 90 17 with with IN cord-104269-9r7rqqqk 90 18 ice ice NN cord-104269-9r7rqqqk 90 19 - - HYPH cord-104269-9r7rqqqk 90 20 cold cold JJ cord-104269-9r7rqqqk 90 21 BSA BSA NNP cord-104269-9r7rqqqk 90 22 - - HYPH cord-104269-9r7rqqqk 90 23 PBS PBS NNP cord-104269-9r7rqqqk 90 24 . . . cord-104269-9r7rqqqk 91 1 Cells cell NNS cord-104269-9r7rqqqk 91 2 from from IN cord-104269-9r7rqqqk 91 3 triplicate triplicate JJ cord-104269-9r7rqqqk 91 4 wells well NNS cord-104269-9r7rqqqk 91 5 for for IN cord-104269-9r7rqqqk 91 6 each each DT cord-104269-9r7rqqqk 91 7 time time NN cord-104269-9r7rqqqk 91 8 point point NN cord-104269-9r7rqqqk 91 9 were be VBD cord-104269-9r7rqqqk 91 10 removed remove VBN cord-104269-9r7rqqqk 91 11 in in IN cord-104269-9r7rqqqk 91 12 0.5 0.5 CD cord-104269-9r7rqqqk 91 13 ml ml NN cord-104269-9r7rqqqk 91 14 1 1 CD cord-104269-9r7rqqqk 91 15 M M NNP cord-104269-9r7rqqqk 91 16 NaOH NaOH NNP cord-104269-9r7rqqqk 91 17 , , , cord-104269-9r7rqqqk 91 18 and and CC cord-104269-9r7rqqqk 91 19 the the DT cord-104269-9r7rqqqk 91 20 radioactivity radioactivity NN cord-104269-9r7rqqqk 91 21 was be VBD cord-104269-9r7rqqqk 91 22 counted count VBN cord-104269-9r7rqqqk 91 23 in in IN cord-104269-9r7rqqqk 91 24 a a DT cord-104269-9r7rqqqk 91 25 gamma gamma NN cord-104269-9r7rqqqk 91 26 counter counter NN cord-104269-9r7rqqqk 91 27 . . . cord-104269-9r7rqqqk 92 1 The the DT cord-104269-9r7rqqqk 92 2 relative relative JJ cord-104269-9r7rqqqk 92 3 levels level NNS cord-104269-9r7rqqqk 92 4 of of IN cord-104269-9r7rqqqk 92 5 wild wild JJ cord-104269-9r7rqqqk 92 6 - - HYPH cord-104269-9r7rqqqk 92 7 type type NN cord-104269-9r7rqqqk 92 8 TR tr NN cord-104269-9r7rqqqk 92 9 and and CC cord-104269-9r7rqqqk 92 10 chimeric chimeric JJ cord-104269-9r7rqqqk 92 11 Ii Ii NNP cord-104269-9r7rqqqk 92 12 - - HYPH cord-104269-9r7rqqqk 92 13 TR TR NNP cord-104269-9r7rqqqk 92 14 constructs construct NNS cord-104269-9r7rqqqk 92 15 expressed express VBN cord-104269-9r7rqqqk 92 16 on on IN cord-104269-9r7rqqqk 92 17 the the DT cord-104269-9r7rqqqk 92 18 various various JJ cord-104269-9r7rqqqk 92 19 CEF CEF NNP cord-104269-9r7rqqqk 92 20 populations population NNS cord-104269-9r7rqqqk 92 21 were be VBD cord-104269-9r7rqqqk 92 22 determined determine VBN cord-104269-9r7rqqqk 92 23 in in IN cord-104269-9r7rqqqk 92 24 each each DT cord-104269-9r7rqqqk 92 25 experiment experiment NN cord-104269-9r7rqqqk 92 26 by by IN cord-104269-9r7rqqqk 92 27 measuring measure VBG cord-104269-9r7rqqqk 92 28 125I 125I NNP cord-104269-9r7rqqqk 92 29 - - HYPH cord-104269-9r7rqqqk 92 30 labeled label VBN cord-104269-9r7rqqqk 92 31 Tf Tf NNP cord-104269-9r7rqqqk 92 32 binding bind VBG cord-104269-9r7rqqqk 92 33 at at IN cord-104269-9r7rqqqk 92 34 4 4 CD cord-104269-9r7rqqqk 92 35 ° ° NN cord-104269-9r7rqqqk 92 36 C c NN cord-104269-9r7rqqqk 92 37 for for IN cord-104269-9r7rqqqk 92 38 each each DT cord-104269-9r7rqqqk 92 39 weft weft NN cord-104269-9r7rqqqk 92 40 . . . cord-104269-9r7rqqqk 93 1 After after IN cord-104269-9r7rqqqk 93 2 preincubation preincubation NN cord-104269-9r7rqqqk 93 3 for for IN cord-104269-9r7rqqqk 93 4 1 1 CD cord-104269-9r7rqqqk 93 5 h h NN cord-104269-9r7rqqqk 93 6 at at IN cord-104269-9r7rqqqk 93 7 37 37 CD cord-104269-9r7rqqqk 93 8 ° ° NN cord-104269-9r7rqqqk 93 9 C c NN cord-104269-9r7rqqqk 93 10 in in IN cord-104269-9r7rqqqk 93 11 serum serum NN cord-104269-9r7rqqqk 93 12 - - HYPH cord-104269-9r7rqqqk 93 13 free free JJ cord-104269-9r7rqqqk 93 14 DME DME NNP cord-104269-9r7rqqqk 93 15 , , , cord-104269-9r7rqqqk 93 16 triplicate triplicate VBZ cord-104269-9r7rqqqk 93 17 wells well NNS cord-104269-9r7rqqqk 93 18 of of IN cord-104269-9r7rqqqk 93 19 cells cell NNS cord-104269-9r7rqqqk 93 20 were be VBD cord-104269-9r7rqqqk 93 21 incubated incubate VBN cord-104269-9r7rqqqk 93 22 with with IN cord-104269-9r7rqqqk 93 23 4/~g 4/~g CD cord-104269-9r7rqqqk 93 24 / / SYM cord-104269-9r7rqqqk 93 25 rul rul NN cord-104269-9r7rqqqk 93 26 t25I t25i RB cord-104269-9r7rqqqk 93 27 - - HYPH cord-104269-9r7rqqqk 93 28 labeled label VBN cord-104269-9r7rqqqk 93 29 Tf Tf NNP cord-104269-9r7rqqqk 93 30 on on IN cord-104269-9r7rqqqk 93 31 ice ice NN cord-104269-9r7rqqqk 93 32 for for IN cord-104269-9r7rqqqk 93 33 1 1 CD cord-104269-9r7rqqqk 93 34 h h NN cord-104269-9r7rqqqk 93 35 , , , cord-104269-9r7rqqqk 93 36 and and CC cord-104269-9r7rqqqk 93 37 then then RB cord-104269-9r7rqqqk 93 38 washed wash VBD cord-104269-9r7rqqqk 93 39 three three CD cord-104269-9r7rqqqk 93 40 times time NNS cord-104269-9r7rqqqk 93 41 with with IN cord-104269-9r7rqqqk 93 42 1 1 CD cord-104269-9r7rqqqk 93 43 mi mi NN cord-104269-9r7rqqqk 93 44 of of IN cord-104269-9r7rqqqk 93 45 ice ice NN cord-104269-9r7rqqqk 93 46 - - HYPH cord-104269-9r7rqqqk 93 47 cold cold JJ cord-104269-9r7rqqqk 93 48 BSA BSA NNP cord-104269-9r7rqqqk 93 49 - - HYPH cord-104269-9r7rqqqk 93 50 PBS PBS NNP cord-104269-9r7rqqqk 93 51 , , , cord-104269-9r7rqqqk 93 52 and and CC cord-104269-9r7rqqqk 93 53 the the DT cord-104269-9r7rqqqk 93 54 radioactivity radioactivity NN cord-104269-9r7rqqqk 93 55 bound bind VBN cord-104269-9r7rqqqk 93 56 to to IN cord-104269-9r7rqqqk 93 57 the the DT cord-104269-9r7rqqqk 93 58 cells cell NNS cord-104269-9r7rqqqk 93 59 was be VBD cord-104269-9r7rqqqk 93 60 counted count VBN cord-104269-9r7rqqqk 93 61 in in IN cord-104269-9r7rqqqk 93 62 a a DT cord-104269-9r7rqqqk 93 63 gamma gamma NN cord-104269-9r7rqqqk 93 64 counter counter NN cord-104269-9r7rqqqk 93 65 . . . cord-104269-9r7rqqqk 94 1 CEF CEF NNP cord-104269-9r7rqqqk 94 2 were be VBD cord-104269-9r7rqqqk 94 3 plated plate VBN cord-104269-9r7rqqqk 94 4 in in IN cord-104269-9r7rqqqk 94 5 triplicate triplicate JJ cord-104269-9r7rqqqk 94 6 wells well NNS cord-104269-9r7rqqqk 94 7 as as IN cord-104269-9r7rqqqk 94 8 described describe VBN cord-104269-9r7rqqqk 94 9 for for IN cord-104269-9r7rqqqk 94 10 the the DT cord-104269-9r7rqqqk 94 11 binding bind VBG cord-104269-9r7rqqqk 94 12 studies study NNS cord-104269-9r7rqqqk 94 13 . . . cord-104269-9r7rqqqk 95 1 Cells cell NNS cord-104269-9r7rqqqk 95 2 were be VBD cord-104269-9r7rqqqk 95 3 preincobated preincobate VBN cord-104269-9r7rqqqk 95 4 in in IN cord-104269-9r7rqqqk 95 5 serum serum NN cord-104269-9r7rqqqk 95 6 - - HYPH cord-104269-9r7rqqqk 95 7 free free JJ cord-104269-9r7rqqqk 95 8 DME DME NNP cord-104269-9r7rqqqk 95 9 for for IN cord-104269-9r7rqqqk 95 10 30 30 CD cord-104269-9r7rqqqk 95 11 vain vain NN cord-104269-9r7rqqqk 95 12 at at IN cord-104269-9r7rqqqk 95 13 37 37 CD cord-104269-9r7rqqqk 95 14 ° ° NN cord-104269-9r7rqqqk 95 15 C c NN cord-104269-9r7rqqqk 95 16 , , , cord-104269-9r7rqqqk 95 17 and and CC cord-104269-9r7rqqqk 95 18 then then RB cord-104269-9r7rqqqk 95 19 incubated incubate VBD cord-104269-9r7rqqqk 95 20 with with IN cord-104269-9r7rqqqk 95 21 125I 125i CD cord-104269-9r7rqqqk 95 22 - - HYPH cord-104269-9r7rqqqk 95 23 labeled label VBN cord-104269-9r7rqqqk 95 24 " " `` cord-104269-9r7rqqqk 95 25 IY IY NNP cord-104269-9r7rqqqk 95 26 ( ( -LRB- cord-104269-9r7rqqqk 95 27 4 4 CD cord-104269-9r7rqqqk 95 28 # # $ cord-104269-9r7rqqqk 95 29 g g NN cord-104269-9r7rqqqk 95 30 / / SYM cord-104269-9r7rqqqk 95 31 m1 m1 NNP cord-104269-9r7rqqqk 95 32 ) ) -RRB- cord-104269-9r7rqqqk 95 33 in in IN cord-104269-9r7rqqqk 95 34 BSA BSA NNP cord-104269-9r7rqqqk 95 35 - - HYPH cord-104269-9r7rqqqk 95 36 PBS PBS NNP cord-104269-9r7rqqqk 95 37 for for IN cord-104269-9r7rqqqk 95 38 1 1 CD cord-104269-9r7rqqqk 95 39 h h NN cord-104269-9r7rqqqk 95 40 at at IN cord-104269-9r7rqqqk 95 41 37 37 CD cord-104269-9r7rqqqk 95 42 ° ° NN cord-104269-9r7rqqqk 95 43 C c NN cord-104269-9r7rqqqk 95 44 . . . cord-104269-9r7rqqqk 96 1 The the DT cord-104269-9r7rqqqk 96 2 medium medium NN cord-104269-9r7rqqqk 96 3 was be VBD cord-104269-9r7rqqqk 96 4 removed remove VBN cord-104269-9r7rqqqk 96 5 and and CC cord-104269-9r7rqqqk 96 6 the the DT cord-104269-9r7rqqqk 96 7 cells cell NNS cord-104269-9r7rqqqk 96 8 were be VBD cord-104269-9r7rqqqk 96 9 washed wash VBN cord-104269-9r7rqqqk 96 10 three three CD cord-104269-9r7rqqqk 96 11 times time NNS cord-104269-9r7rqqqk 96 12 with with IN cord-104269-9r7rqqqk 96 13 ice ice NN cord-104269-9r7rqqqk 96 14 - - HYPH cord-104269-9r7rqqqk 96 15 cold cold JJ cord-104269-9r7rqqqk 96 16 BSA BSA NNP cord-104269-9r7rqqqk 96 17 - - HYPH cord-104269-9r7rqqqk 96 18 PBS PBS NNP cord-104269-9r7rqqqk 96 19 and and CC cord-104269-9r7rqqqk 96 20 incubated incubate VBD cord-104269-9r7rqqqk 96 21 at at IN cord-104269-9r7rqqqk 96 22 37 37 CD cord-104269-9r7rqqqk 96 23 ° ° NN cord-104269-9r7rqqqk 96 24 C c NN cord-104269-9r7rqqqk 96 25 with with IN cord-104269-9r7rqqqk 96 26 prewarmed prewarmed NNP cord-104269-9r7rqqqk 96 27 ( ( -LRB- cord-104269-9r7rqqqk 96 28 37 37 CD cord-104269-9r7rqqqk 96 29 ° ° NN cord-104269-9r7rqqqk 96 30 C C NNP cord-104269-9r7rqqqk 96 31 ) ) -RRB- cord-104269-9r7rqqqk 96 32 DME DME NNP cord-104269-9r7rqqqk 96 33 containing contain VBG cord-104269-9r7rqqqk 96 34 0.1 0.1 CD cord-104269-9r7rqqqk 96 35 % % NN cord-104269-9r7rqqqk 96 36 BSA BSA NNP cord-104269-9r7rqqqk 96 37 and and CC cord-104269-9r7rqqqk 96 38 50 50 CD cord-104269-9r7rqqqk 96 39 # # NN cord-104269-9r7rqqqk 96 40 g g NN cord-104269-9r7rqqqk 96 41 / / SYM cord-104269-9r7rqqqk 96 42 ml ml NN cord-104269-9r7rqqqk 96 43 unlabeled unlabeled JJ cord-104269-9r7rqqqk 96 44 " " `` cord-104269-9r7rqqqk 96 45 If if IN cord-104269-9r7rqqqk 96 46 for for IN cord-104269-9r7rqqqk 96 47 0 0 CD cord-104269-9r7rqqqk 96 48 , , , cord-104269-9r7rqqqk 96 49 5 5 CD cord-104269-9r7rqqqk 96 50 , , , cord-104269-9r7rqqqk 96 51 10 10 CD cord-104269-9r7rqqqk 96 52 , , , cord-104269-9r7rqqqk 96 53 15 15 CD cord-104269-9r7rqqqk 96 54 , , , cord-104269-9r7rqqqk 96 55 20 20 CD cord-104269-9r7rqqqk 96 56 , , , cord-104269-9r7rqqqk 96 57 40 40 CD cord-104269-9r7rqqqk 96 58 , , , cord-104269-9r7rqqqk 96 59 or or CC cord-104269-9r7rqqqk 96 60 60 60 CD cord-104269-9r7rqqqk 96 61 rain rain NN cord-104269-9r7rqqqk 96 62 . . . cord-104269-9r7rqqqk 97 1 After after IN cord-104269-9r7rqqqk 97 2 incubation incubation NN cord-104269-9r7rqqqk 97 3 , , , cord-104269-9r7rqqqk 97 4 the the DT cord-104269-9r7rqqqk 97 5 medium medium NN cord-104269-9r7rqqqk 97 6 was be VBD cord-104269-9r7rqqqk 97 7 collected collect VBN cord-104269-9r7rqqqk 97 8 , , , cord-104269-9r7rqqqk 97 9 protein protein NN cord-104269-9r7rqqqk 97 10 was be VBD cord-104269-9r7rqqqk 97 11 precipitated precipitate VBN cord-104269-9r7rqqqk 97 12 in in IN cord-104269-9r7rqqqk 97 13 10 10 CD cord-104269-9r7rqqqk 97 14 % % NN cord-104269-9r7rqqqk 97 15 TCA TCA NNP cord-104269-9r7rqqqk 97 16 and and CC cord-104269-9r7rqqqk 97 17 removed remove VBN cord-104269-9r7rqqqk 97 18 by by IN cord-104269-9r7rqqqk 97 19 centrifugation centrifugation NN cord-104269-9r7rqqqk 97 20 , , , cord-104269-9r7rqqqk 97 21 and and CC cord-104269-9r7rqqqk 97 22 then then RB cord-104269-9r7rqqqk 97 23 the the DT cord-104269-9r7rqqqk 97 24 acid acid NN cord-104269-9r7rqqqk 97 25 - - HYPH cord-104269-9r7rqqqk 97 26 soluble soluble JJ cord-104269-9r7rqqqk 97 27 and and CC cord-104269-9r7rqqqk 97 28 acid acid NN cord-104269-9r7rqqqk 97 29 - - HYPH cord-104269-9r7rqqqk 97 30 insoluble insoluble JJ cord-104269-9r7rqqqk 97 31 radioactivity radioactivity NN cord-104269-9r7rqqqk 97 32 was be VBD cord-104269-9r7rqqqk 97 33 counted count VBN cord-104269-9r7rqqqk 97 34 in in IN cord-104269-9r7rqqqk 97 35 a a DT cord-104269-9r7rqqqk 97 36 gamma gamma NN cord-104269-9r7rqqqk 97 37 counter counter NN cord-104269-9r7rqqqk 97 38 . . . cord-104269-9r7rqqqk 98 1 The the DT cord-104269-9r7rqqqk 98 2 surfacebound surfacebound NN cord-104269-9r7rqqqk 98 3 and and CC cord-104269-9r7rqqqk 98 4 internalized internalize VBD cord-104269-9r7rqqqk 98 5 " " '' cord-104269-9r7rqqqk 99 1 If if IN cord-104269-9r7rqqqk 99 2 in in IN cord-104269-9r7rqqqk 99 3 CEF CEF NNP cord-104269-9r7rqqqk 99 4 was be VBD cord-104269-9r7rqqqk 99 5 determined determine VBN cord-104269-9r7rqqqk 99 6 by by IN cord-104269-9r7rqqqk 99 7 the the DT cord-104269-9r7rqqqk 99 8 acid acid NN cord-104269-9r7rqqqk 99 9 wash wash NN cord-104269-9r7rqqqk 99 10 procedure procedure NN cord-104269-9r7rqqqk 99 11 described describe VBN cord-104269-9r7rqqqk 99 12 for for IN cord-104269-9r7rqqqk 99 13 the the DT cord-104269-9r7rqqqk 99 14 steady steady JJ cord-104269-9r7rqqqk 99 15 - - HYPH cord-104269-9r7rqqqk 99 16 state state NN cord-104269-9r7rqqqk 99 17 distribution distribution NN cord-104269-9r7rqqqk 99 18 assay assay NN cord-104269-9r7rqqqk 99 19 . . . cord-104269-9r7rqqqk 100 1 CEF CEF NNP cord-104269-9r7rqqqk 100 2 expressing express VBG cord-104269-9r7rqqqk 100 3 chimeric chimeric JJ cord-104269-9r7rqqqk 100 4 Ii Ii NNP cord-104269-9r7rqqqk 100 5 - - HYPH cord-104269-9r7rqqqk 100 6 TR TR NNP cord-104269-9r7rqqqk 100 7 constructs construct NNS cord-104269-9r7rqqqk 100 8 were be VBD cord-104269-9r7rqqqk 100 9 1~I 1~i CD cord-104269-9r7rqqqk 100 10 surface surface NN cord-104269-9r7rqqqk 100 11 - - HYPH cord-104269-9r7rqqqk 100 12 labeled label VBN cord-104269-9r7rqqqk 100 13 using use VBG cord-104269-9r7rqqqk 100 14 lactoperoxidase lactoperoxidase NNP cord-104269-9r7rqqqk 100 15 for for IN cord-104269-9r7rqqqk 100 16 10 10 CD cord-104269-9r7rqqqk 100 17 min min NN cord-104269-9r7rqqqk 100 18 essentially essentially RB cord-104269-9r7rqqqk 100 19 as as IN cord-104269-9r7rqqqk 100 20 described describe VBN cord-104269-9r7rqqqk 100 21 , , , cord-104269-9r7rqqqk 100 22 except except IN cord-104269-9r7rqqqk 100 23 that that DT cord-104269-9r7rqqqk 100 24 iodination iodination NN cord-104269-9r7rqqqk 100 25 was be VBD cord-104269-9r7rqqqk 100 26 performed perform VBN cord-104269-9r7rqqqk 100 27 in in IN cord-104269-9r7rqqqk 100 28 6-cm 6-cm CD cord-104269-9r7rqqqk 100 29 tissue tissue NN cord-104269-9r7rqqqk 100 30 culture culture NN cord-104269-9r7rqqqk 100 31 dishes dish NNS cord-104269-9r7rqqqk 100 32 in in IN cord-104269-9r7rqqqk 100 33 a a DT cord-104269-9r7rqqqk 100 34 total total JJ cord-104269-9r7rqqqk 100 35 volume volume NN cord-104269-9r7rqqqk 100 36 of of IN cord-104269-9r7rqqqk 100 37 0.5 0.5 CD cord-104269-9r7rqqqk 100 38 rnl rnl NNP cord-104269-9r7rqqqk 100 39 . . . cord-104269-9r7rqqqk 101 1 CEF CEF NNP cord-104269-9r7rqqqk 101 2 were be VBD cord-104269-9r7rqqqk 101 3 then then RB cord-104269-9r7rqqqk 101 4 washed wash VBN cord-104269-9r7rqqqk 101 5 four four CD cord-104269-9r7rqqqk 101 6 times time NNS cord-104269-9r7rqqqk 101 7 with with IN cord-104269-9r7rqqqk 101 8 ice ice NN cord-104269-9r7rqqqk 101 9 - - : cord-104269-9r7rqqqk 101 10 culd culd NNP cord-104269-9r7rqqqk 101 11 PBS PBS NNP cord-104269-9r7rqqqk 101 12 and and CC cord-104269-9r7rqqqk 101 13 incubated incubate VBD cord-104269-9r7rqqqk 101 14 at at IN cord-104269-9r7rqqqk 101 15 37 37 CD cord-104269-9r7rqqqk 101 16 ° ° NN cord-104269-9r7rqqqk 101 17 C c NN cord-104269-9r7rqqqk 101 18 in in IN cord-104269-9r7rqqqk 101 19 complete complete JJ cord-104269-9r7rqqqk 101 20 medium medium NN cord-104269-9r7rqqqk 101 21 for for IN cord-104269-9r7rqqqk 101 22 0 0 CD cord-104269-9r7rqqqk 101 23 , , , cord-104269-9r7rqqqk 101 24 2 2 CD cord-104269-9r7rqqqk 101 25 , , , cord-104269-9r7rqqqk 101 26 3 3 CD cord-104269-9r7rqqqk 101 27 , , , cord-104269-9r7rqqqk 101 28 4 4 CD cord-104269-9r7rqqqk 101 29 , , , cord-104269-9r7rqqqk 101 30 or or CC cord-104269-9r7rqqqk 101 31 6 6 CD cord-104269-9r7rqqqk 101 32 h. h. NN cord-104269-9r7rqqqk 102 1 At at IN cord-104269-9r7rqqqk 102 2 each each DT cord-104269-9r7rqqqk 102 3 time time NN cord-104269-9r7rqqqk 102 4 point point NN cord-104269-9r7rqqqk 102 5 , , , cord-104269-9r7rqqqk 102 6 cells cell NNS cord-104269-9r7rqqqk 102 7 were be VBD cord-104269-9r7rqqqk 102 8 solub'dized solub'dize VBN cord-104269-9r7rqqqk 102 9 on on IN cord-104269-9r7rqqqk 102 10 ice ice NN cord-104269-9r7rqqqk 102 11 with with IN cord-104269-9r7rqqqk 102 12 1 1 CD cord-104269-9r7rqqqk 102 13 % % NN cord-104269-9r7rqqqk 102 14 NP-40-PBS np-40-pbs NN cord-104269-9r7rqqqk 102 15 , , , cord-104269-9r7rqqqk 102 16 and and CC cord-104269-9r7rqqqk 102 17 the the DT cord-104269-9r7rqqqk 102 18 chimeric chimeric JJ cord-104269-9r7rqqqk 102 19 Ii Ii NNP cord-104269-9r7rqqqk 102 20 - - HYPH cord-104269-9r7rqqqk 102 21 TR TR NNP cord-104269-9r7rqqqk 102 22 constructs construct NNS cord-104269-9r7rqqqk 102 23 were be VBD cord-104269-9r7rqqqk 102 24 immunoprecipitated immunoprecipitate VBN cord-104269-9r7rqqqk 102 25 using use VBG cord-104269-9r7rqqqk 102 26 B3/25 b3/25 JJ cord-104269-9r7rqqqk 102 27 monoclonal monoclonal JJ cord-104269-9r7rqqqk 102 28 antibody antibody NN cord-104269-9r7rqqqk 102 29 specific specific JJ cord-104269-9r7rqqqk 102 30 for for IN cord-104269-9r7rqqqk 102 31 the the DT cord-104269-9r7rqqqk 102 32 extracellular extracellular JJ cord-104269-9r7rqqqk 102 33 domain domain NN cord-104269-9r7rqqqk 102 34 of of IN cord-104269-9r7rqqqk 102 35 the the DT cord-104269-9r7rqqqk 102 36 human human JJ cord-104269-9r7rqqqk 102 37 TR tr NN cord-104269-9r7rqqqk 102 38 . . . cord-104269-9r7rqqqk 103 1 Immunoprecipitates Immunoprecipitates NNP cord-104269-9r7rqqqk 103 2 were be VBD cord-104269-9r7rqqqk 103 3 analyzed analyze VBN cord-104269-9r7rqqqk 103 4 on on IN cord-104269-9r7rqqqk 103 5 7.5 7.5 CD cord-104269-9r7rqqqk 103 6 % % NN cord-104269-9r7rqqqk 103 7 SDS sds NN cord-104269-9r7rqqqk 103 8 - - HYPH cord-104269-9r7rqqqk 103 9 polyacrylamide polyacrylamide NN cord-104269-9r7rqqqk 103 10 gels gel NNS cord-104269-9r7rqqqk 103 11 , , , cord-104269-9r7rqqqk 103 12 which which WDT cord-104269-9r7rqqqk 103 13 were be VBD cord-104269-9r7rqqqk 103 14 then then RB cord-104269-9r7rqqqk 103 15 dried dry VBN cord-104269-9r7rqqqk 103 16 and and CC cord-104269-9r7rqqqk 103 17 exposed expose VBN cord-104269-9r7rqqqk 103 18 to to IN cord-104269-9r7rqqqk 103 19 preflashed preflashed JJ cord-104269-9r7rqqqk 103 20 XAR XAR NNP cord-104269-9r7rqqqk 103 21 film film NN cord-104269-9r7rqqqk 103 22 ( ( -LRB- cord-104269-9r7rqqqk 103 23 Eastman Eastman NNP cord-104269-9r7rqqqk 103 24 Kodak Kodak NNP cord-104269-9r7rqqqk 103 25 , , , cord-104269-9r7rqqqk 103 26 Rochester Rochester NNP cord-104269-9r7rqqqk 103 27 , , , cord-104269-9r7rqqqk 103 28 NY NY NNP cord-104269-9r7rqqqk 103 29 ) ) -RRB- cord-104269-9r7rqqqk 103 30 . . . cord-104269-9r7rqqqk 104 1 CEF CEF NNP cord-104269-9r7rqqqk 104 2 were be VBD cord-104269-9r7rqqqk 104 3 washed wash VBN cord-104269-9r7rqqqk 104 4 twice twice RB cord-104269-9r7rqqqk 104 5 with with IN cord-104269-9r7rqqqk 104 6 methionine methionine NN cord-104269-9r7rqqqk 104 7 - - HYPH cord-104269-9r7rqqqk 104 8 free free JJ cord-104269-9r7rqqqk 104 9 DME DME NNP cord-104269-9r7rqqqk 104 10 , , , cord-104269-9r7rqqqk 104 11 and and CC cord-104269-9r7rqqqk 104 12 then then RB cord-104269-9r7rqqqk 104 13 pulse pulse NN cord-104269-9r7rqqqk 104 14 - - HYPH cord-104269-9r7rqqqk 104 15 labeled label VBN cord-104269-9r7rqqqk 104 16 for for IN cord-104269-9r7rqqqk 104 17 30 30 CD cord-104269-9r7rqqqk 104 18 rain rain NN cord-104269-9r7rqqqk 104 19 in in IN cord-104269-9r7rqqqk 104 20 1 1 CD cord-104269-9r7rqqqk 104 21 ml ml NNS cord-104269-9r7rqqqk 104 22 of of IN cord-104269-9r7rqqqk 104 23 DME DME NNP cord-104269-9r7rqqqk 104 24 containing contain VBG cord-104269-9r7rqqqk 104 25 0.12 0.12 CD cord-104269-9r7rqqqk 104 26 mCi mCi NNPS cord-104269-9r7rqqqk 104 27 / / SYM cord-104269-9r7rqqqk 104 28 mi mi JJ cord-104269-9r7rqqqk 104 29 trans trans NNP cord-104269-9r7rqqqk 104 30 - - HYPH cord-104269-9r7rqqqk 104 31 asS as NNS cord-104269-9r7rqqqk 104 32 - - HYPH cord-104269-9r7rqqqk 104 33 label label NN cord-104269-9r7rqqqk 104 34 ( ( -LRB- cord-104269-9r7rqqqk 104 35 ICN ICN NNP cord-104269-9r7rqqqk 104 36 Biomedicals Biomedicals NNP cord-104269-9r7rqqqk 104 37 , , , cord-104269-9r7rqqqk 104 38 Irvine Irvine NNP cord-104269-9r7rqqqk 104 39 , , , cord-104269-9r7rqqqk 104 40 CA CA NNP cord-104269-9r7rqqqk 104 41 ) ) -RRB- cord-104269-9r7rqqqk 104 42 and and CC cord-104269-9r7rqqqk 104 43 2 2 CD cord-104269-9r7rqqqk 104 44 % % NN cord-104269-9r7rqqqk 104 45 defined define VBN cord-104269-9r7rqqqk 104 46 calf calf NN cord-104269-9r7rqqqk 104 47 serum serum NN cord-104269-9r7rqqqk 104 48 . . . cord-104269-9r7rqqqk 105 1 Pulse pulse NN cord-104269-9r7rqqqk 105 2 - - HYPH cord-104269-9r7rqqqk 105 3 labeled label VBN cord-104269-9r7rqqqk 105 4 cells cell NNS cord-104269-9r7rqqqk 105 5 were be VBD cord-104269-9r7rqqqk 105 6 chased chase VBN cord-104269-9r7rqqqk 105 7 for for IN cord-104269-9r7rqqqk 105 8 0 0 CD cord-104269-9r7rqqqk 105 9 , , , cord-104269-9r7rqqqk 105 10 2 2 CD cord-104269-9r7rqqqk 105 11 , , , cord-104269-9r7rqqqk 105 12 4 4 CD cord-104269-9r7rqqqk 105 13 , , , cord-104269-9r7rqqqk 105 14 8 8 CD cord-104269-9r7rqqqk 105 15 , , , cord-104269-9r7rqqqk 105 16 or or CC cord-104269-9r7rqqqk 105 17 24 24 CD cord-104269-9r7rqqqk 105 18 h h NN cord-104269-9r7rqqqk 105 19 in in IN cord-104269-9r7rqqqk 105 20 complete complete JJ cord-104269-9r7rqqqk 105 21 medium medium NN cord-104269-9r7rqqqk 105 22 . . . cord-104269-9r7rqqqk 106 1 In in IN cord-104269-9r7rqqqk 106 2 one one CD cord-104269-9r7rqqqk 106 3 experiment experiment NN cord-104269-9r7rqqqk 106 4 , , , cord-104269-9r7rqqqk 106 5 cells cell NNS cord-104269-9r7rqqqk 106 6 were be VBD cord-104269-9r7rqqqk 106 7 preincubated preincubate VBN cord-104269-9r7rqqqk 106 8 for for IN cord-104269-9r7rqqqk 106 9 4 4 CD cord-104269-9r7rqqqk 106 10 h h NN cord-104269-9r7rqqqk 106 11 in in IN cord-104269-9r7rqqqk 106 12 medium medium NN cord-104269-9r7rqqqk 106 13 containing contain VBG cord-104269-9r7rqqqk 106 14 100 100 CD cord-104269-9r7rqqqk 106 15 # # $ cord-104269-9r7rqqqk 106 16 g g NN cord-104269-9r7rqqqk 106 17 / / SYM cord-104269-9r7rqqqk 106 18 ml ml NN cord-104269-9r7rqqqk 106 19 leupeptin leupeptin NN cord-104269-9r7rqqqk 106 20 , , , cord-104269-9r7rqqqk 106 21 and and CC cord-104269-9r7rqqqk 106 22 then then RB cord-104269-9r7rqqqk 106 23 pulse pulse NN cord-104269-9r7rqqqk 106 24 - - HYPH cord-104269-9r7rqqqk 106 25 labeled label VBN cord-104269-9r7rqqqk 106 26 and and CC cord-104269-9r7rqqqk 106 27 chased chase VBN cord-104269-9r7rqqqk 106 28 for for IN cord-104269-9r7rqqqk 106 29 0 0 CD cord-104269-9r7rqqqk 106 30 , , , cord-104269-9r7rqqqk 106 31 1 1 CD cord-104269-9r7rqqqk 106 32 , , , cord-104269-9r7rqqqk 106 33 2 2 CD cord-104269-9r7rqqqk 106 34 , , , cord-104269-9r7rqqqk 106 35 3 3 CD cord-104269-9r7rqqqk 106 36 , , , cord-104269-9r7rqqqk 106 37 or or CC cord-104269-9r7rqqqk 106 38 4 4 CD cord-104269-9r7rqqqk 106 39 h h NN cord-104269-9r7rqqqk 106 40 in in IN cord-104269-9r7rqqqk 106 41 leupeptin leupeptin NN cord-104269-9r7rqqqk 106 42 - - HYPH cord-104269-9r7rqqqk 106 43 containing contain VBG cord-104269-9r7rqqqk 106 44 medium medium NN cord-104269-9r7rqqqk 106 45 . . . cord-104269-9r7rqqqk 107 1 At at IN cord-104269-9r7rqqqk 107 2 each each DT cord-104269-9r7rqqqk 107 3 time time NN cord-104269-9r7rqqqk 107 4 point point NN cord-104269-9r7rqqqk 107 5 , , , cord-104269-9r7rqqqk 107 6 cells cell NNS cord-104269-9r7rqqqk 107 7 were be VBD cord-104269-9r7rqqqk 107 8 soluffdized soluffdize VBN cord-104269-9r7rqqqk 107 9 on on IN cord-104269-9r7rqqqk 107 10 ice ice NN cord-104269-9r7rqqqk 107 11 with with IN cord-104269-9r7rqqqk 107 12 1 1 CD cord-104269-9r7rqqqk 107 13 % % NN cord-104269-9r7rqqqk 107 14 NP-40-PBS np-40-pbs NN cord-104269-9r7rqqqk 107 15 . . . cord-104269-9r7rqqqk 108 1 The the DT cord-104269-9r7rqqqk 108 2 wild wild JJ cord-104269-9r7rqqqk 108 3 - - HYPH cord-104269-9r7rqqqk 108 4 type type NN cord-104269-9r7rqqqk 108 5 TR tr NN cord-104269-9r7rqqqk 108 6 and and CC cord-104269-9r7rqqqk 108 7 chimeric chimeric JJ cord-104269-9r7rqqqk 108 8 fi fi NN cord-104269-9r7rqqqk 108 9 - - HYPH cord-104269-9r7rqqqk 108 10 TR tr NN cord-104269-9r7rqqqk 108 11 constructs construct NNS cord-104269-9r7rqqqk 108 12 were be VBD cord-104269-9r7rqqqk 108 13 immunoprecipitated immunoprecipitate VBN cord-104269-9r7rqqqk 108 14 from from IN cord-104269-9r7rqqqk 108 15 postnuclear postnuclear NNP cord-104269-9r7rqqqk 108 16 supernatants supernatant NNS cord-104269-9r7rqqqk 108 17 using use VBG cord-104269-9r7rqqqk 108 18 B3/25 b3/25 JJ cord-104269-9r7rqqqk 108 19 monoclonal monoclonal JJ cord-104269-9r7rqqqk 108 20 antibody antibody NN cord-104269-9r7rqqqk 108 21 and and CC cord-104269-9r7rqqqk 108 22 analyzed analyze VBD cord-104269-9r7rqqqk 108 23 on on IN cord-104269-9r7rqqqk 108 24 7.5 7.5 CD cord-104269-9r7rqqqk 108 25 % % NN cord-104269-9r7rqqqk 108 26 SDS sds NN cord-104269-9r7rqqqk 108 27 - - HYPH cord-104269-9r7rqqqk 108 28 polyacrylamide polyacrylamide NN cord-104269-9r7rqqqk 108 29 gels gel NNS cord-104269-9r7rqqqk 108 30 . . . cord-104269-9r7rqqqk 109 1 Dried dry VBN cord-104269-9r7rqqqk 109 2 gels gel NNS cord-104269-9r7rqqqk 109 3 were be VBD cord-104269-9r7rqqqk 109 4 exposed expose VBN cord-104269-9r7rqqqk 109 5 to to IN cord-104269-9r7rqqqk 109 6 prettashed prettashe VBN cord-104269-9r7rqqqk 109 7 XAR XAR NNP cord-104269-9r7rqqqk 109 8 film film NN cord-104269-9r7rqqqk 109 9 ( ( -LRB- cord-104269-9r7rqqqk 109 10 Eastman Eastman NNP cord-104269-9r7rqqqk 109 11 Kodak Kodak NNP cord-104269-9r7rqqqk 109 12 , , , cord-104269-9r7rqqqk 109 13 Rochester Rochester NNP cord-104269-9r7rqqqk 109 14 , , , cord-104269-9r7rqqqk 109 15 NY NY NNP cord-104269-9r7rqqqk 109 16 ) ) -RRB- cord-104269-9r7rqqqk 109 17 . . . cord-104269-9r7rqqqk 110 1 Quantitation quantitation NN cord-104269-9r7rqqqk 110 2 of of IN cord-104269-9r7rqqqk 110 3 radioactivity radioactivity NN cord-104269-9r7rqqqk 110 4 was be VBD cord-104269-9r7rqqqk 110 5 performed perform VBN cord-104269-9r7rqqqk 110 6 on on IN cord-104269-9r7rqqqk 110 7 a a DT cord-104269-9r7rqqqk 110 8 model model NN cord-104269-9r7rqqqk 110 9 425 425 CD cord-104269-9r7rqqqk 110 10 PhosphorImager PhosphorImager NNP cord-104269-9r7rqqqk 110 11 ( ( -LRB- cord-104269-9r7rqqqk 110 12 Molecular Molecular NNP cord-104269-9r7rqqqk 110 13 Dynamics Dynamics NNPS cord-104269-9r7rqqqk 110 14 , , , cord-104269-9r7rqqqk 110 15 Sunnyvale Sunnyvale NNP cord-104269-9r7rqqqk 110 16 , , , cord-104269-9r7rqqqk 110 17 CA CA NNP cord-104269-9r7rqqqk 110 18 ) ) -RRB- cord-104269-9r7rqqqk 110 19 . . . cord-104269-9r7rqqqk 111 1 The the DT cord-104269-9r7rqqqk 111 2 surface surface NN cord-104269-9r7rqqqk 111 3 - - HYPH cord-104269-9r7rqqqk 111 4 accessible accessible JJ cord-104269-9r7rqqqk 111 5 pools pool NNS cord-104269-9r7rqqqk 111 6 of of IN cord-104269-9r7rqqqk 111 7 wild wild JJ cord-104269-9r7rqqqk 111 8 - - HYPH cord-104269-9r7rqqqk 111 9 type type NN cord-104269-9r7rqqqk 111 10 TR tr NN cord-104269-9r7rqqqk 111 11 and and CC cord-104269-9r7rqqqk 111 12 Ii Ii NNP cord-104269-9r7rqqqk 111 13 - - HYPH cord-104269-9r7rqqqk 111 14 TR TR NNP cord-104269-9r7rqqqk 111 15 chimeras chimera NNS cord-104269-9r7rqqqk 111 16 were be VBD cord-104269-9r7rqqqk 111 17 calculated calculate VBN cord-104269-9r7rqqqk 111 18 from from IN cord-104269-9r7rqqqk 111 19 the the DT cord-104269-9r7rqqqk 111 20 nsI nsI NNS cord-104269-9r7rqqqk 111 21 - - HYPH cord-104269-9r7rqqqk 111 22 labeled label VBN cord-104269-9r7rqqqk 111 23 " " `` cord-104269-9r7rqqqk 111 24 If if IN cord-104269-9r7rqqqk 111 25 bound bind VBN cord-104269-9r7rqqqk 111 26 to to IN cord-104269-9r7rqqqk 111 27 intracellular intracellular JJ cord-104269-9r7rqqqk 111 28 and and CC cord-104269-9r7rqqqk 111 29 surface surface NN cord-104269-9r7rqqqk 111 30 receptors receptor NNS cord-104269-9r7rqqqk 111 31 at at IN cord-104269-9r7rqqqk 111 32 steady steady JJ cord-104269-9r7rqqqk 111 33 - - HYPH cord-104269-9r7rqqqk 111 34 state state NN cord-104269-9r7rqqqk 111 35 . . . cord-104269-9r7rqqqk 112 1 The the DT cord-104269-9r7rqqqk 112 2 surface surface NN cord-104269-9r7rqqqk 112 3 - - HYPH cord-104269-9r7rqqqk 112 4 accessible accessible JJ cord-104269-9r7rqqqk 112 5 pool pool NN cord-104269-9r7rqqqk 112 6 of of IN cord-104269-9r7rqqqk 112 7 chimera chimera NN cord-104269-9r7rqqqk 112 8 relative relative JJ cord-104269-9r7rqqqk 112 9 to to IN cord-104269-9r7rqqqk 112 10 wild4ype wild4ype NN cord-104269-9r7rqqqk 112 11 TR TR NNP cord-104269-9r7rqqqk 112 12 , , , cord-104269-9r7rqqqk 112 13 [ [ -LRB- cord-104269-9r7rqqqk 112 14 Ii Ii NNP cord-104269-9r7rqqqk 112 15 - - HYPH cord-104269-9r7rqqqk 112 16 TR TR NNP cord-104269-9r7rqqqk 112 17 ] ] -RRB- cord-104269-9r7rqqqk 112 18 s^ s^ NNS cord-104269-9r7rqqqk 112 19 , , , cord-104269-9r7rqqqk 112 20 equals equal VBZ cord-104269-9r7rqqqk 112 21 the the DT cord-104269-9r7rqqqk 112 22 cell cell NN cord-104269-9r7rqqqk 112 23 surface surface NN cord-104269-9r7rqqqk 112 24 chimem chimem NNP cord-104269-9r7rqqqk 112 25 - - HYPH cord-104269-9r7rqqqk 112 26 Tf Tf NNP cord-104269-9r7rqqqk 112 27 complexes complex NNS cord-104269-9r7rqqqk 112 28 , , , cord-104269-9r7rqqqk 112 29 FIi fii NN cord-104269-9r7rqqqk 112 30 - - HYPH cord-104269-9r7rqqqk 112 31 TR]s~ TR]s~ NNP cord-104269-9r7rqqqk 112 32 , , , cord-104269-9r7rqqqk 112 33 plus plus CC cord-104269-9r7rqqqk 112 34 internalized internalize VBN cord-104269-9r7rqqqk 112 35 chimera chimera NN cord-104269-9r7rqqqk 112 36 - - HYPH cord-104269-9r7rqqqk 112 37 Tf Tf NNP cord-104269-9r7rqqqk 112 38 complexes complex NNS cord-104269-9r7rqqqk 113 1 [ [ -LRB- cord-104269-9r7rqqqk 113 2 Ii Ii NNP cord-104269-9r7rqqqk 113 3 - - HYPH cord-104269-9r7rqqqk 113 4 TR TR NNP cord-104269-9r7rqqqk 113 5 ] ] -RRB- cord-104269-9r7rqqqk 113 6 int int NN cord-104269-9r7rqqqk 113 7 , , , cord-104269-9r7rqqqk 113 8 multiplied multiply VBN cord-104269-9r7rqqqk 113 9 by by IN cord-104269-9r7rqqqk 113 10 100 100 CD cord-104269-9r7rqqqk 113 11 , , , cord-104269-9r7rqqqk 113 12 divided divide VBN cord-104269-9r7rqqqk 113 13 by by IN cord-104269-9r7rqqqk 113 14 cell cell NN cord-104269-9r7rqqqk 113 15 surface surface NN cord-104269-9r7rqqqk 113 16 TR TR NNP cord-104269-9r7rqqqk 113 17 - - HYPH cord-104269-9r7rqqqk 113 18 Tf Tf NNP cord-104269-9r7rqqqk 113 19 complexes complex NNS cord-104269-9r7rqqqk 113 20 [ [ -LRB- cord-104269-9r7rqqqk 113 21 TR]sur TR]sur NNP cord-104269-9r7rqqqk 113 22 , , , cord-104269-9r7rqqqk 113 23 plus plus CC cord-104269-9r7rqqqk 113 24 internalized internalize VBN cord-104269-9r7rqqqk 113 25 TR TR NNP cord-104269-9r7rqqqk 113 26 - - HYPH cord-104269-9r7rqqqk 113 27 Tf Tf NNP cord-104269-9r7rqqqk 113 28 complexes complex NNS cord-104269-9r7rqqqk 113 29 , , , cord-104269-9r7rqqqk 114 1 [ [ -LRB- cord-104269-9r7rqqqk 114 2 TR]int TR]int NNP cord-104269-9r7rqqqk 114 3 ; ; , cord-104269-9r7rqqqk 114 4 i.e. i.e. FW cord-104269-9r7rqqqk 114 5 , , , cord-104269-9r7rqqqk 114 6 [ [ -LRB- cord-104269-9r7rqqqk 114 7 Ii Ii NNP cord-104269-9r7rqqqk 114 8 - - HYPH cord-104269-9r7rqqqk 114 9 TR TR NNP cord-104269-9r7rqqqk 114 10 ] ] -RRB- cord-104269-9r7rqqqk 114 11 _SP cord-104269-9r7rqqqk 114 12 receptors receptor NNS cord-104269-9r7rqqqk 114 13 . . . cord-104269-9r7rqqqk 115 1 This this DT cord-104269-9r7rqqqk 115 2 condition condition NN cord-104269-9r7rqqqk 115 3 is be VBZ cord-104269-9r7rqqqk 115 4 met meet VBN cord-104269-9r7rqqqk 115 5 as as IN cord-104269-9r7rqqqk 115 6 steady steady JJ cord-104269-9r7rqqqk 115 7 - - HYPH cord-104269-9r7rqqqk 115 8 state state NN cord-104269-9r7rqqqk 115 9 conditions condition NNS cord-104269-9r7rqqqk 115 10 are be VBP cord-104269-9r7rqqqk 115 11 achieved achieve VBN cord-104269-9r7rqqqk 115 12 in in IN cord-104269-9r7rqqqk 115 13 , , , cord-104269-9r7rqqqk 115 14 ~20 ~20 NFP cord-104269-9r7rqqqk 115 15 rain rain NN cord-104269-9r7rqqqk 115 16 at at IN cord-104269-9r7rqqqk 115 17 37 37 CD cord-104269-9r7rqqqk 115 18 ° ° NN cord-104269-9r7rqqqk 115 19 C c NN cord-104269-9r7rqqqk 115 20 ( ( -LRB- cord-104269-9r7rqqqk 115 21 fling fling NN cord-104269-9r7rqqqk 115 22 et et FW cord-104269-9r7rqqqk 115 23 al al NNP cord-104269-9r7rqqqk 115 24 . . NNP cord-104269-9r7rqqqk 115 25 , , , cord-104269-9r7rqqqk 115 26 1990 1990 CD cord-104269-9r7rqqqk 115 27 ; ; : cord-104269-9r7rqqqk 115 28 see see VB cord-104269-9r7rqqqk 115 29 also also RB cord-104269-9r7rqqqk 115 30 above above RB cord-104269-9r7rqqqk 115 31 ) ) -RRB- cord-104269-9r7rqqqk 115 32 . . . cord-104269-9r7rqqqk 116 1 In in IN cord-104269-9r7rqqqk 116 2 20 20 CD cord-104269-9r7rqqqk 116 3 rain rain NN cord-104269-9r7rqqqk 116 4 , , , cord-104269-9r7rqqqk 116 5 < < XX cord-104269-9r7rqqqk 116 6 10 10 CD cord-104269-9r7rqqqk 116 7 % % NN cord-104269-9r7rqqqk 116 8 of of IN cord-104269-9r7rqqqk 116 9 the the DT cord-104269-9r7rqqqk 116 10 total total NN cord-104269-9r7rqqqk 116 11 125Llabeled 125llabele VBN cord-104269-9r7rqqqk 116 12 " " '' cord-104269-9r7rqqqk 116 13 If if IN cord-104269-9r7rqqqk 116 14 bound bind VBN cord-104269-9r7rqqqk 116 15 to to IN cord-104269-9r7rqqqk 116 16 the the DT cord-104269-9r7rqqqk 116 17 most most RBS cord-104269-9r7rqqqk 116 18 rapidly rapidly RB cord-104269-9r7rqqqk 116 19 degraded degrade VBN cord-104269-9r7rqqqk 116 20 chimera chimera NN cord-104269-9r7rqqqk 116 21 , , , cord-104269-9r7rqqqk 116 22 Iicr+xu Iicr+xu NNP cord-104269-9r7rqqqk 116 23 , , , cord-104269-9r7rqqqk 116 24 is be VBZ cord-104269-9r7rqqqk 116 25 released release VBN cord-104269-9r7rqqqk 116 26 into into IN cord-104269-9r7rqqqk 116 27 the the DT cord-104269-9r7rqqqk 116 28 medium medium NN cord-104269-9r7rqqqk 116 29 as as IN cord-104269-9r7rqqqk 116 30 ~ ~ NFP cord-104269-9r7rqqqk 116 31 soluble soluble JJ cord-104269-9r7rqqqk 116 32 material material NN cord-104269-9r7rqqqk 116 33 ( ( -LRB- cord-104269-9r7rqqqk 116 34 see see VB cord-104269-9r7rqqqk 116 35 Fig Fig NNP cord-104269-9r7rqqqk 116 36 . . NNP cord-104269-9r7rqqqk 116 37 7 7 CD cord-104269-9r7rqqqk 116 38 ) ) -RRB- cord-104269-9r7rqqqk 116 39 . . . cord-104269-9r7rqqqk 117 1 The the DT cord-104269-9r7rqqqk 117 2 fraction fraction NN cord-104269-9r7rqqqk 117 3 of of IN cord-104269-9r7rqqqk 117 4 Ii Ii NNP cord-104269-9r7rqqqk 117 5 - - HYPH cord-104269-9r7rqqqk 117 6 TR TR NNP cord-104269-9r7rqqqk 117 7 chimeras chimera NNS cord-104269-9r7rqqqk 117 8 trafficking traffic VBG cord-104269-9r7rqqqk 117 9 via via IN cord-104269-9r7rqqqk 117 10 the the DT cord-104269-9r7rqqqk 117 11 cell cell NN cord-104269-9r7rqqqk 117 12 surface surface NN cord-104269-9r7rqqqk 117 13 is be VBZ cord-104269-9r7rqqqk 117 14 the the DT cord-104269-9r7rqqqk 117 15 surface surface NN cord-104269-9r7rqqqk 117 16 accessible accessible JJ cord-104269-9r7rqqqk 117 17 pool pool NN cord-104269-9r7rqqqk 117 18 , , , cord-104269-9r7rqqqk 117 19 [ [ -LRB- cord-104269-9r7rqqqk 117 20 Ii Ii NNP cord-104269-9r7rqqqk 117 21 - - HYPH cord-104269-9r7rqqqk 117 22 TR TR NNP cord-104269-9r7rqqqk 117 23 ] ] -RRB- cord-104269-9r7rqqqk 117 24 sA sA NNP cord-104269-9r7rqqqk 117 25 , , , cord-104269-9r7rqqqk 117 26 divided divide VBN cord-104269-9r7rqqqk 117 27 by by IN cord-104269-9r7rqqqk 117 28 the the DT cord-104269-9r7rqqqk 117 29 rate rate NN cord-104269-9r7rqqqk 117 30 of of IN cord-104269-9r7rqqqk 117 31 biosynthesis biosynthesis NN cord-104269-9r7rqqqk 117 32 of of IN cord-104269-9r7rqqqk 117 33 the the DT cord-104269-9r7rqqqk 117 34 Ii Ii NNP cord-104269-9r7rqqqk 117 35 - - HYPH cord-104269-9r7rqqqk 117 36 TR TR NNP cord-104269-9r7rqqqk 117 37 chimera chimera NN cord-104269-9r7rqqqk 117 38 multiplied multiply VBN cord-104269-9r7rqqqk 117 39 by by IN cord-104269-9r7rqqqk 117 40 the the DT cord-104269-9r7rqqqk 117 41 rate rate NN cord-104269-9r7rqqqk 117 42 of of IN cord-104269-9r7rqqqk 117 43 degradation degradation NN cord-104269-9r7rqqqk 117 44 of of IN cord-104269-9r7rqqqk 117 45 [ [ -LRB- cord-104269-9r7rqqqk 117 46 Ii Ii NNP cord-104269-9r7rqqqk 117 47 - - HYPH cord-104269-9r7rqqqk 117 48 TR TR NNP cord-104269-9r7rqqqk 117 49 ] ] -RRB- cord-104269-9r7rqqqk 117 50 sA sa NN cord-104269-9r7rqqqk 117 51 ; ; , cord-104269-9r7rqqqk 117 52 i.e. i.e. FW cord-104269-9r7rqqqk 117 53 , , , cord-104269-9r7rqqqk 117 54 ( ( -LRB- cord-104269-9r7rqqqk 117 55 [ [ -LRB- cord-104269-9r7rqqqk 117 56 Ii Ii NNP cord-104269-9r7rqqqk 117 57 - - HYPH cord-104269-9r7rqqqk 117 58 TRsA TRsA NNP cord-104269-9r7rqqqk 117 59 X x NN cord-104269-9r7rqqqk 117 60 degradation degradation NN cord-104269-9r7rqqqk 117 61 rate rate NN cord-104269-9r7rqqqk 117 62 of of IN cord-104269-9r7rqqqk 117 63 synthesis synthesis NN cord-104269-9r7rqqqk 117 64 . . . cord-104269-9r7rqqqk 118 1 As as IN cord-104269-9r7rqqqk 118 2 an an DT cord-104269-9r7rqqqk 118 3 example example NN cord-104269-9r7rqqqk 118 4 of of IN cord-104269-9r7rqqqk 118 5 the the DT cord-104269-9r7rqqqk 118 6 calculation calculation NN cord-104269-9r7rqqqk 118 7 , , , cord-104269-9r7rqqqk 118 8 the the DT cord-104269-9r7rqqqk 118 9 surface surface NN cord-104269-9r7rqqqk 118 10 expression expression NN cord-104269-9r7rqqqk 118 11 of of IN cord-104269-9r7rqqqk 118 12 Iic'r+TM Iic'r+TM NNP cord-104269-9r7rqqqk 118 13 is be VBZ cord-104269-9r7rqqqk 118 14 12 12 CD cord-104269-9r7rqqqk 118 15 % % NN cord-104269-9r7rqqqk 118 16 relative relative JJ cord-104269-9r7rqqqk 118 17 to to IN cord-104269-9r7rqqqk 118 18 wild wild JJ cord-104269-9r7rqqqk 118 19 - - HYPH cord-104269-9r7rqqqk 118 20 type type NN cord-104269-9r7rqqqk 118 21 TR tr NN cord-104269-9r7rqqqk 118 22 , , , cord-104269-9r7rqqqk 118 23 and and CC cord-104269-9r7rqqqk 118 24 at at IN cord-104269-9r7rqqqk 118 25 stendy stendy NNP cord-104269-9r7rqqqk 118 26 - - HYPH cord-104269-9r7rqqqk 118 27 state state NN cord-104269-9r7rqqqk 118 28 76 76 CD cord-104269-9r7rqqqk 118 29 % % NN cord-104269-9r7rqqqk 118 30 of of IN cord-104269-9r7rqqqk 118 31 the the DT cord-104269-9r7rqqqk 118 32 Iicr+~ Iicr+~ NNP cord-104269-9r7rqqqk 118 33 chimera chimera NNP cord-104269-9r7rqqqk 118 34 and and CC cord-104269-9r7rqqqk 118 35 62 62 CD cord-104269-9r7rqqqk 118 36 % % NN cord-104269-9r7rqqqk 118 37 of of IN cord-104269-9r7rqqqk 118 38 the the DT cord-104269-9r7rqqqk 118 39 wild wild JJ cord-104269-9r7rqqqk 118 40 - - HYPH cord-104269-9r7rqqqk 118 41 type type NN cord-104269-9r7rqqqk 118 42 TR tr NN cord-104269-9r7rqqqk 118 43 are be VBP cord-104269-9r7rqqqk 118 44 internalized internalize VBN cord-104269-9r7rqqqk 118 45 ( ( -LRB- cord-104269-9r7rqqqk 118 46 see see VB cord-104269-9r7rqqqk 118 47 Results result NNS cord-104269-9r7rqqqk 118 48 and and CC cord-104269-9r7rqqqk 118 49 Table Table NNP cord-104269-9r7rqqqk 118 50 _SP cord-104269-9r7rqqqk 118 51 I I NNP cord-104269-9r7rqqqk 118 52 ) ) -RRB- cord-104269-9r7rqqqk 118 53 . . . cord-104269-9r7rqqqk 119 1 Thus thus RB cord-104269-9r7rqqqk 119 2 , , , cord-104269-9r7rqqqk 119 3 [ [ -LRB- cord-104269-9r7rqqqk 119 4 Iic'r+T~.dSA Iic'r+T~.dSA NNP cord-104269-9r7rqqqk 119 5 ffi ffi NNP cord-104269-9r7rqqqk 119 6 ( ( -LRB- cord-104269-9r7rqqqk 119 7 12 12 CD cord-104269-9r7rqqqk 119 8 + + SYM cord-104269-9r7rqqqk 119 9 [ [ -LRB- cord-104269-9r7rqqqk 119 10 12 12 CD cord-104269-9r7rqqqk 119 11 X x CD cord-104269-9r7rqqqk 119 12 76/24 76/24 CD cord-104269-9r7rqqqk 119 13 ] ] -RRB- cord-104269-9r7rqqqk 119 14 ) ) -RRB- cord-104269-9r7rqqqk 120 1 X X NNS cord-104269-9r7rqqqk 120 2 100/(100 100/(100 CD cord-104269-9r7rqqqk 121 1 + + CC cord-104269-9r7rqqqk 122 1 [ [ -LRB- cord-104269-9r7rqqqk 122 2 100 100 CD cord-104269-9r7rqqqk 122 3 X x NN cord-104269-9r7rqqqk 122 4 62/38 62/38 CD cord-104269-9r7rqqqk 122 5 ] ] -RRB- cord-104269-9r7rqqqk 122 6 ) ) -RRB- cord-104269-9r7rqqqk 122 7 = = NFP cord-104269-9r7rqqqk 123 1 19 19 CD cord-104269-9r7rqqqk 123 2 % % NN cord-104269-9r7rqqqk 123 3 . . . cord-104269-9r7rqqqk 124 1 Quantitation quantitation NN cord-104269-9r7rqqqk 124 2 of of IN cord-104269-9r7rqqqk 124 3 the the DT cord-104269-9r7rqqqk 124 4 data datum NNS cord-104269-9r7rqqqk 124 5 presented present VBN cord-104269-9r7rqqqk 124 6 in in IN cord-104269-9r7rqqqk 124 7 Fig Fig NNP cord-104269-9r7rqqqk 124 8 . . NNP cord-104269-9r7rqqqk 124 9 2 2 CD cord-104269-9r7rqqqk 124 10 showed show VBD cord-104269-9r7rqqqk 124 11 that that IN cord-104269-9r7rqqqk 124 12 eightfold eightfold RB cord-104269-9r7rqqqk 124 13 more more JJR cord-104269-9r7rqqqk 124 14 Iic~+~ iic~+~ JJ cord-104269-9r7rqqqk 124 15 chimera chimera NN cord-104269-9r7rqqqk 124 16 was be VBD cord-104269-9r7rqqqk 124 17 synthesized synthesize VBN cord-104269-9r7rqqqk 124 18 than than IN cord-104269-9r7rqqqk 124 19 wild wild JJ cord-104269-9r7rqqqk 124 20 - - HYPH cord-104269-9r7rqqqk 124 21 type type NN cord-104269-9r7rqqqk 124 22 TR tr NN cord-104269-9r7rqqqk 124 23 , , , cord-104269-9r7rqqqk 124 24 whereas whereas IN cord-104269-9r7rqqqk 124 25 quantitation quantitation NN cord-104269-9r7rqqqk 124 26 of of IN cord-104269-9r7rqqqk 124 27 the the DT cord-104269-9r7rqqqk 124 28 data datum NNS cord-104269-9r7rqqqk 124 29 presented present VBN cord-104269-9r7rqqqk 124 30 in in IN cord-104269-9r7rqqqk 124 31 Fig Fig NNP cord-104269-9r7rqqqk 124 32 . . . cord-104269-9r7rqqqk 125 1 8 8 CD cord-104269-9r7rqqqk 125 2 showed show VBD cord-104269-9r7rqqqk 125 3 that that IN cord-104269-9r7rqqqk 125 4 the the DT cord-104269-9r7rqqqk 125 5 [ [ -LRB- cord-104269-9r7rqqqk 125 6 Iic'r+TM]SA iic'r+tm]sa NN cord-104269-9r7rqqqk 125 7 is be VBZ cord-104269-9r7rqqqk 125 8 degraded degrade VBN cord-104269-9r7rqqqk 125 9 eightfold eightfold JJ cord-104269-9r7rqqqk 125 10 faster fast RBR cord-104269-9r7rqqqk 125 11 than than IN cord-104269-9r7rqqqk 125 12 wild wild JJ cord-104269-9r7rqqqk 125 13 - - HYPH cord-104269-9r7rqqqk 125 14 type type NN cord-104269-9r7rqqqk 125 15 TR tr NN cord-104269-9r7rqqqk 125 16 . . . cord-104269-9r7rqqqk 126 1 Therefore therefore RB cord-104269-9r7rqqqk 126 2 , , , cord-104269-9r7rqqqk 126 3 the the DT cord-104269-9r7rqqqk 126 4 fraction fraction NN cord-104269-9r7rqqqk 126 5 of of IN cord-104269-9r7rqqqk 126 6 Iic'r+TM Iic'r+TM NNP cord-104269-9r7rqqqk 126 7 chimeras chimera NNS cord-104269-9r7rqqqk 126 8 trafficking traffic VBG cord-104269-9r7rqqqk 126 9 via via IN cord-104269-9r7rqqqk 126 10 the the DT cord-104269-9r7rqqqk 126 11 cell cell NN cord-104269-9r7rqqqk 126 12 surface surface NN cord-104269-9r7rqqqk 126 13 relative relative JJ cord-104269-9r7rqqqk 126 14 to to IN cord-104269-9r7rqqqk 126 15 wildtype wildtype JJ cord-104269-9r7rqqqk 126 16 TR tr NN cord-104269-9r7rqqqk 126 17 is be VBZ cord-104269-9r7rqqqk 126 18 19 19 CD cord-104269-9r7rqqqk 126 19 x x SYM cord-104269-9r7rqqqk 126 20 8/8 8/8 CD cord-104269-9r7rqqqk 126 21 % % NN cord-104269-9r7rqqqk 126 22 or or CC cord-104269-9r7rqqqk 126 23 19 19 CD cord-104269-9r7rqqqk 126 24 % % NN cord-104269-9r7rqqqk 126 25 . . . cord-104269-9r7rqqqk 127 1 CEF CEF NNP cord-104269-9r7rqqqk 127 2 and and CC cord-104269-9r7rqqqk 127 3 CEF CEF NNP cord-104269-9r7rqqqk 127 4 expressing express VBG cord-104269-9r7rqqqk 127 5 wild wild JJ cord-104269-9r7rqqqk 127 6 - - HYPH cord-104269-9r7rqqqk 127 7 type type NN cord-104269-9r7rqqqk 127 8 or or CC cord-104269-9r7rqqqk 127 9 Ii Ii NNP cord-104269-9r7rqqqk 127 10 - - HYPH cord-104269-9r7rqqqk 127 11 TR TR NNP cord-104269-9r7rqqqk 127 12 chimeras chimera NNS cord-104269-9r7rqqqk 127 13 were be VBD cord-104269-9r7rqqqk 127 14 plated plate VBN cord-104269-9r7rqqqk 127 15 onto onto IN cord-104269-9r7rqqqk 127 16 glass glass NN cord-104269-9r7rqqqk 127 17 coverslips coverslip NNS cord-104269-9r7rqqqk 127 18 and and CC cord-104269-9r7rqqqk 127 19 cultured culture VBN cord-104269-9r7rqqqk 127 20 overnight overnight RB cord-104269-9r7rqqqk 127 21 . . . cord-104269-9r7rqqqk 128 1 The the DT cord-104269-9r7rqqqk 128 2 cells cell NNS cord-104269-9r7rqqqk 128 3 were be VBD cord-104269-9r7rqqqk 128 4 fixed fix VBN cord-104269-9r7rqqqk 128 5 with with IN cord-104269-9r7rqqqk 128 6 2 2 CD cord-104269-9r7rqqqk 128 7 % % NN cord-104269-9r7rqqqk 128 8 formaldehyde formaldehyde NN cord-104269-9r7rqqqk 128 9 treatment treatment NN cord-104269-9r7rqqqk 128 10 for for IN cord-104269-9r7rqqqk 128 11 15 15 CD cord-104269-9r7rqqqk 128 12 rain rain NN cord-104269-9r7rqqqk 128 13 at at IN cord-104269-9r7rqqqk 128 14 room room NN cord-104269-9r7rqqqk 128 15 temperature temperature NN cord-104269-9r7rqqqk 128 16 ( ( -LRB- cord-104269-9r7rqqqk 128 17 RT RT NNP cord-104269-9r7rqqqk 128 18 ) ) -RRB- cord-104269-9r7rqqqk 128 19 , , , cord-104269-9r7rqqqk 128 20 quenched quench VBN cord-104269-9r7rqqqk 128 21 in in IN cord-104269-9r7rqqqk 128 22 0.27 0.27 CD cord-104269-9r7rqqqk 128 23 % % NN cord-104269-9r7rqqqk 128 24 NI ni NN cord-104269-9r7rqqqk 128 25 - - HYPH cord-104269-9r7rqqqk 128 26 hC1 hC1 NNP cord-104269-9r7rqqqk 128 27 , , , cord-104269-9r7rqqqk 128 28 0.37 0.37 CD cord-104269-9r7rqqqk 128 29 % % NN cord-104269-9r7rqqqk 128 30 glycine glycine NN cord-104269-9r7rqqqk 128 31 , , , cord-104269-9r7rqqqk 128 32 pH pH NNP cord-104269-9r7rqqqk 128 33 7.2 7.2 CD cord-104269-9r7rqqqk 128 34 , , , cord-104269-9r7rqqqk 128 35 and and CC cord-104269-9r7rqqqk 128 36 incubated incubate VBN cord-104269-9r7rqqqk 128 37 in in IN cord-104269-9r7rqqqk 128 38 P p NN cord-104269-9r7rqqqk 128 39 / / SYM cord-104269-9r7rqqqk 128 40 H H NNP cord-104269-9r7rqqqk 128 41 / / SYM cord-104269-9r7rqqqk 128 42 S S NNP cord-104269-9r7rqqqk 128 43 ( ( -LRB- cord-104269-9r7rqqqk 128 44 PBS/10 PBS/10 NNP cord-104269-9r7rqqqk 128 45 % % NN cord-104269-9r7rqqqk 128 46 horse horse NN cord-104269-9r7rqqqk 128 47 serum/0.1 serum/0.1 NNP cord-104269-9r7rqqqk 128 48 % % NN cord-104269-9r7rqqqk 128 49 saponin saponin NN cord-104269-9r7rqqqk 128 50 ) ) -RRB- cord-104269-9r7rqqqk 128 51 ( ( -LRB- cord-104269-9r7rqqqk 128 52 for for IN cord-104269-9r7rqqqk 128 53 permeabilized permeabilized JJ cord-104269-9r7rqqqk 128 54 samples sample NNS cord-104269-9r7rqqqk 128 55 ) ) -RRB- cord-104269-9r7rqqqk 128 56 or or CC cord-104269-9r7rqqqk 128 57 P p NN cord-104269-9r7rqqqk 128 58 / / SYM cord-104269-9r7rqqqk 128 59 H h NN cord-104269-9r7rqqqk 128 60 ( ( -LRB- cord-104269-9r7rqqqk 128 61 for for IN cord-104269-9r7rqqqk 128 62 nonpermeab'diznd nonpermeab'diznd DT cord-104269-9r7rqqqk 128 63 samples sample NNS cord-104269-9r7rqqqk 128 64 ) ) -RRB- cord-104269-9r7rqqqk 128 65 for for IN cord-104269-9r7rqqqk 128 66 30 30 CD cord-104269-9r7rqqqk 128 67 min min NN cord-104269-9r7rqqqk 128 68 at at IN cord-104269-9r7rqqqk 128 69 RT RT NNP cord-104269-9r7rqqqk 128 70 . . . cord-104269-9r7rqqqk 129 1 The the DT cord-104269-9r7rqqqk 129 2 samples sample NNS cord-104269-9r7rqqqk 129 3 were be VBD cord-104269-9r7rqqqk 129 4 next next RB cord-104269-9r7rqqqk 129 5 incubated incubate VBN cord-104269-9r7rqqqk 129 6 with with IN cord-104269-9r7rqqqk 129 7 monoclonal monoclonal JJ cord-104269-9r7rqqqk 129 8 antibody antibody NN cord-104269-9r7rqqqk 130 1 B3/25 B3/25 NNP cord-104269-9r7rqqqk 131 1 ( ( -LRB- cord-104269-9r7rqqqk 131 2 undiluted undiluted JJ cord-104269-9r7rqqqk 131 3 hybridoma hybridoma NN cord-104269-9r7rqqqk 131 4 supernatants supernatant NNS cord-104269-9r7rqqqk 131 5 ) ) -RRB- cord-104269-9r7rqqqk 131 6 followed follow VBN cord-104269-9r7rqqqk 131 7 by by IN cord-104269-9r7rqqqk 131 8 a a DT cord-104269-9r7rqqqk 131 9 second second JJ cord-104269-9r7rqqqk 131 10 incubation incubation NN cord-104269-9r7rqqqk 131 11 with with IN cord-104269-9r7rqqqk 131 12 rat rat NN cord-104269-9r7rqqqk 131 13 anti anti JJ cord-104269-9r7rqqqk 131 14 - - JJ cord-104269-9r7rqqqk 131 15 mouse mouse NN cord-104269-9r7rqqqk 131 16 IgGi IgGi NNP cord-104269-9r7rqqqk 131 17 - - HYPH cord-104269-9r7rqqqk 131 18 FrIC FrIC NNP cord-104269-9r7rqqqk 131 19 ( ( -LRB- cord-104269-9r7rqqqk 131 20 1:20 1:20 CD cord-104269-9r7rqqqk 131 21 dilution dilution NN cord-104269-9r7rqqqk 131 22 ; ; : cord-104269-9r7rqqqk 131 23 Zymed Zymed NNP cord-104269-9r7rqqqk 131 24 Labs Labs NNP cord-104269-9r7rqqqk 131 25 . . . cord-104269-9r7rqqqk 131 26 ) ) -RRB- cord-104269-9r7rqqqk 131 27 . . . cord-104269-9r7rqqqk 132 1 Each each DT cord-104269-9r7rqqqk 132 2 incubation incubation NN cord-104269-9r7rqqqk 132 3 step step NN cord-104269-9r7rqqqk 132 4 was be VBD cord-104269-9r7rqqqk 132 5 followed follow VBN cord-104269-9r7rqqqk 132 6 by by IN cord-104269-9r7rqqqk 132 7 three three CD cord-104269-9r7rqqqk 132 8 washes wash NNS cord-104269-9r7rqqqk 132 9 in in IN cord-104269-9r7rqqqk 132 10 P p NN cord-104269-9r7rqqqk 132 11 / / SYM cord-104269-9r7rqqqk 132 12 H H NNP cord-104269-9r7rqqqk 132 13 / / SYM cord-104269-9r7rqqqk 132 14 S S NNP cord-104269-9r7rqqqk 132 15 or or CC cord-104269-9r7rqqqk 132 16 P p NN cord-104269-9r7rqqqk 132 17 / / SYM cord-104269-9r7rqqqk 132 18 H h NN cord-104269-9r7rqqqk 132 19 for for IN cord-104269-9r7rqqqk 132 20 10 10 CD cord-104269-9r7rqqqk 132 21 rain rain NN cord-104269-9r7rqqqk 132 22 each each DT cord-104269-9r7rqqqk 132 23 . . . cord-104269-9r7rqqqk 133 1 In in IN cord-104269-9r7rqqqk 133 2 all all DT cord-104269-9r7rqqqk 133 3 cases case NNS cord-104269-9r7rqqqk 133 4 , , , cord-104269-9r7rqqqk 133 5 antibodies antibody NNS cord-104269-9r7rqqqk 133 6 were be VBD cord-104269-9r7rqqqk 133 7 diluted dilute VBN cord-104269-9r7rqqqk 133 8 in in IN cord-104269-9r7rqqqk 133 9 P p NN cord-104269-9r7rqqqk 133 10 / / SYM cord-104269-9r7rqqqk 133 11 I I NNP cord-104269-9r7rqqqk 133 12 - - HYPH cord-104269-9r7rqqqk 133 13 US US NNP cord-104269-9r7rqqqk 133 14 or or CC cord-104269-9r7rqqqk 133 15 P P NNP cord-104269-9r7rqqqk 133 16 / / SYM cord-104269-9r7rqqqk 133 17 H H NNP cord-104269-9r7rqqqk 133 18 , , , cord-104269-9r7rqqqk 133 19 and and CC cord-104269-9r7rqqqk 133 20 primary primary JJ cord-104269-9r7rqqqk 133 21 and and CC cord-104269-9r7rqqqk 133 22 secondary secondary JJ cord-104269-9r7rqqqk 133 23 antibodies antibody NNS cord-104269-9r7rqqqk 133 24 were be VBD cord-104269-9r7rqqqk 133 25 incubated incubate VBN cord-104269-9r7rqqqk 133 26 for for IN cord-104269-9r7rqqqk 133 27 1 1 CD cord-104269-9r7rqqqk 133 28 h h NN cord-104269-9r7rqqqk 133 29 and and CC cord-104269-9r7rqqqk 133 30 30 30 CD cord-104269-9r7rqqqk 133 31 rain rain NN cord-104269-9r7rqqqk 133 32 , , , cord-104269-9r7rqqqk 133 33 respectively respectively RB cord-104269-9r7rqqqk 133 34 . . . cord-104269-9r7rqqqk 134 1 Covershps Covershps NNP cord-104269-9r7rqqqk 134 2 were be VBD cord-104269-9r7rqqqk 134 3 mounted mount VBN cord-104269-9r7rqqqk 134 4 in in IN cord-104269-9r7rqqqk 134 5 1 1 CD cord-104269-9r7rqqqk 134 6 mg mg NNP cord-104269-9r7rqqqk 134 7 / / SYM cord-104269-9r7rqqqk 134 8 ml ml NN cord-104269-9r7rqqqk 134 9 p p NN cord-104269-9r7rqqqk 134 10 - - HYPH cord-104269-9r7rqqqk 134 11 phenylenediamine phenylenediamine NN cord-104269-9r7rqqqk 134 12 in in IN cord-104269-9r7rqqqk 134 13 a a DT cord-104269-9r7rqqqk 134 14 1:10 1:10 CD cord-104269-9r7rqqqk 134 15 mixture mixture NN cord-104269-9r7rqqqk 134 16 of of IN cord-104269-9r7rqqqk 134 17 PBS PBS NNP cord-104269-9r7rqqqk 134 18 : : : cord-104269-9r7rqqqk 135 1 glycerol glycerol NN cord-104269-9r7rqqqk 135 2 and and CC cord-104269-9r7rqqqk 135 3 sealed seal VBN cord-104269-9r7rqqqk 135 4 with with IN cord-104269-9r7rqqqk 135 5 nail nail NN cord-104269-9r7rqqqk 135 6 polish polish NN cord-104269-9r7rqqqk 135 7 . . . cord-104269-9r7rqqqk 136 1 Slides slide NNS cord-104269-9r7rqqqk 136 2 were be VBD cord-104269-9r7rqqqk 136 3 analyzed analyze VBN cord-104269-9r7rqqqk 136 4 on on IN cord-104269-9r7rqqqk 136 5 a a DT cord-104269-9r7rqqqk 136 6 Nikon Nikon NNP cord-104269-9r7rqqqk 136 7 Optiphot Optiphot NNP cord-104269-9r7rqqqk 136 8 microscope microscope NN cord-104269-9r7rqqqk 136 9 equipped equip VBN cord-104269-9r7rqqqk 136 10 with with IN cord-104269-9r7rqqqk 136 11 epifluorescence epifluorescence NN cord-104269-9r7rqqqk 136 12 and and CC cord-104269-9r7rqqqk 136 13 a a DT cord-104269-9r7rqqqk 136 14 DAGE DAGE NNP cord-104269-9r7rqqqk 136 15 MTI MTI NNP cord-104269-9r7rqqqk 136 16 series series NN cord-104269-9r7rqqqk 136 17 SIT sit VB cord-104269-9r7rqqqk 136 18 video video NN cord-104269-9r7rqqqk 136 19 camera camera NN cord-104269-9r7rqqqk 137 1 ( ( -LRB- cord-104269-9r7rqqqk 137 2 Dage Dage NNP cord-104269-9r7rqqqk 137 3 - - HYPH cord-104269-9r7rqqqk 137 4 MTI MTI NNP cord-104269-9r7rqqqk 137 5 , , , cord-104269-9r7rqqqk 137 6 Michigan Michigan NNP cord-104269-9r7rqqqk 137 7 City City NNP cord-104269-9r7rqqqk 137 8 , , , cord-104269-9r7rqqqk 137 9 IN in IN cord-104269-9r7rqqqk 137 10 ) ) -RRB- cord-104269-9r7rqqqk 137 11 coupled couple VBN cord-104269-9r7rqqqk 137 12 to to IN cord-104269-9r7rqqqk 137 13 an an DT cord-104269-9r7rqqqk 137 14 Image image NN cord-104269-9r7rqqqk 137 15 1-AT 1-at CD cord-104269-9r7rqqqk 138 1 ( ( -LRB- cord-104269-9r7rqqqk 138 2 Universal Universal NNP cord-104269-9r7rqqqk 138 3 Ima Ima NNP cord-104269-9r7rqqqk 138 4 ~ ~ NFP cord-104269-9r7rqqqk 138 5 ng~ ng~ NNP cord-104269-9r7rqqqk 138 6 Media Media NNP cord-104269-9r7rqqqk 138 7 , , , cord-104269-9r7rqqqk 138 8 PA PA NNP cord-104269-9r7rqqqk 138 9 ) ) -RRB- cord-104269-9r7rqqqk 138 10 image image NN cord-104269-9r7rqqqk 138 11 analysis analysis NN cord-104269-9r7rqqqk 138 12 system system NN cord-104269-9r7rqqqk 138 13 . . . cord-104269-9r7rqqqk 139 1 Micrographs Micrographs NNP cord-104269-9r7rqqqk 139 2 were be VBD cord-104269-9r7rqqqk 139 3 prepared prepare VBN cord-104269-9r7rqqqk 139 4 with with IN cord-104269-9r7rqqqk 139 5 a a DT cord-104269-9r7rqqqk 139 6 Sony Sony NNP cord-104269-9r7rqqqk 139 7 Color Color NNP cord-104269-9r7rqqqk 139 8 Video video NN cord-104269-9r7rqqqk 139 9 Printer Printer NNP cord-104269-9r7rqqqk 139 10 ( ( -LRB- cord-104269-9r7rqqqk 139 11 UP-5000 up-5000 RB cord-104269-9r7rqqqk 139 12 ) ) -RRB- cord-104269-9r7rqqqk 139 13 . . . cord-104269-9r7rqqqk 140 1 For for IN cord-104269-9r7rqqqk 140 2 double double JJ cord-104269-9r7rqqqk 140 3 - - HYPH cord-104269-9r7rqqqk 140 4 label label NN cord-104269-9r7rqqqk 140 5 indirect indirect JJ cord-104269-9r7rqqqk 140 6 immunofluorescence immunofluorescence NN cord-104269-9r7rqqqk 140 7 , , , cord-104269-9r7rqqqk 140 8 ceils ceil NNS cord-104269-9r7rqqqk 140 9 were be VBD cord-104269-9r7rqqqk 140 10 fixed fix VBN cord-104269-9r7rqqqk 140 11 , , , cord-104269-9r7rqqqk 140 12 permeabilized permeabilized JJ cord-104269-9r7rqqqk 140 13 , , , cord-104269-9r7rqqqk 140 14 and and CC cord-104269-9r7rqqqk 140 15 incubated incubate VBN cord-104269-9r7rqqqk 140 16 with with IN cord-104269-9r7rqqqk 140 17 the the DT cord-104269-9r7rqqqk 140 18 following follow VBG cord-104269-9r7rqqqk 140 19 combinations combination NNS cord-104269-9r7rqqqk 140 20 of of IN cord-104269-9r7rqqqk 140 21 antibodies antibody NNS cord-104269-9r7rqqqk 140 22 : : : cord-104269-9r7rqqqk 140 23 rabbit rabbit NNP cord-104269-9r7rqqqk 140 24 anti anti JJ cord-104269-9r7rqqqk 140 25 - - JJ cord-104269-9r7rqqqk 140 26 human human JJ cord-104269-9r7rqqqk 140 27 TR tr NN cord-104269-9r7rqqqk 140 28 antiserum antiserum NN cord-104269-9r7rqqqk 140 29 ( ( -LRB- cord-104269-9r7rqqqk 140 30 1:500 1:500 CD cord-104269-9r7rqqqk 140 31 dilution dilution NN cord-104269-9r7rqqqk 140 32 ) ) -RRB- cord-104269-9r7rqqqk 140 33 , , , cord-104269-9r7rqqqk 140 34 goat goat NN cord-104269-9r7rqqqk 140 35 anti anti JJ cord-104269-9r7rqqqk 140 36 - - JJ cord-104269-9r7rqqqk 140 37 rabbit rabbit JJ cord-104269-9r7rqqqk 140 38 Ig Ig NNP cord-104269-9r7rqqqk 140 39 - - HYPH cord-104269-9r7rqqqk 140 40 FITC FITC NNP cord-104269-9r7rqqqk 140 41 ( ( -LRB- cord-104269-9r7rqqqk 140 42 1:20 1:20 CD cord-104269-9r7rqqqk 140 43 dilution dilution NN cord-104269-9r7rqqqk 140 44 ; ; : cord-104269-9r7rqqqk 140 45 American American NNP cord-104269-9r7rqqqk 140 46 Qualex Qualex NNP cord-104269-9r7rqqqk 140 47 ) ) -RRB- cord-104269-9r7rqqqk 140 48 , , , cord-104269-9r7rqqqk 140 49 mouse mouse NN cord-104269-9r7rqqqk 140 50 monoclonal monoclonal JJ cord-104269-9r7rqqqk 140 51 antibody antibody NN cord-104269-9r7rqqqk 140 52 anti anti NNS cord-104269-9r7rqqqk 140 53 - - NNS cord-104269-9r7rqqqk 140 54 LEP100 lep100 JJ cord-104269-9r7rqqqk 140 55 ( ( -LRB- cord-104269-9r7rqqqk 140 56 undiluted undiluted JJ cord-104269-9r7rqqqk 140 57 tissue tissue NN cord-104269-9r7rqqqk 140 58 culture culture NN cord-104269-9r7rqqqk 140 59 supernatant supernatant NN cord-104269-9r7rqqqk 140 60 , , , cord-104269-9r7rqqqk 140 61 Developmental Developmental NNP cord-104269-9r7rqqqk 140 62 Studies Studies NNPS cord-104269-9r7rqqqk 140 63 Hybridoma Hybridoma NNP cord-104269-9r7rqqqk 140 64 Bank Bank NNP cord-104269-9r7rqqqk 140 65 , , , cord-104269-9r7rqqqk 140 66 University University NNP cord-104269-9r7rqqqk 140 67 of of IN cord-104269-9r7rqqqk 140 68 Iowa Iowa NNP cord-104269-9r7rqqqk 140 69 ) ) -RRB- cord-104269-9r7rqqqk 140 70 , , , cord-104269-9r7rqqqk 140 71 goat goat NNP cord-104269-9r7rqqqk 140 72 anti anti JJ cord-104269-9r7rqqqk 140 73 - - JJ cord-104269-9r7rqqqk 140 74 mouse mouse JJ cord-104269-9r7rqqqk 140 75 IgG IgG NNP cord-104269-9r7rqqqk 140 76 - - HYPH cord-104269-9r7rqqqk 140 77 Texas Texas NNP cord-104269-9r7rqqqk 140 78 red red NN cord-104269-9r7rqqqk 140 79 ( ( -LRB- cord-104269-9r7rqqqk 140 80 1:100 1:100 CD cord-104269-9r7rqqqk 140 81 : : : cord-104269-9r7rqqqk 140 82 Zymed Zymed NNP cord-104269-9r7rqqqk 140 83 Laboratories Laboratories NNP cord-104269-9r7rqqqk 140 84 ) ) -RRB- cord-104269-9r7rqqqk 140 85 . . . cord-104269-9r7rqqqk 141 1 Slides slide NNS cord-104269-9r7rqqqk 141 2 were be VBD cord-104269-9r7rqqqk 141 3 analyzed analyze VBN cord-104269-9r7rqqqk 141 4 on on IN cord-104269-9r7rqqqk 141 5 a a DT cord-104269-9r7rqqqk 141 6 Leitz Leitz NNP cord-104269-9r7rqqqk 141 7 fluorescence fluorescence NN cord-104269-9r7rqqqk 141 8 microscope microscope NN cord-104269-9r7rqqqk 141 9 equipped equip VBN cord-104269-9r7rqqqk 141 10 with with IN cord-104269-9r7rqqqk 141 11 a a DT cord-104269-9r7rqqqk 141 12 Vario Vario NNP cord-104269-9r7rqqqk 141 13 Orthomat Orthomat NNP cord-104269-9r7rqqqk 141 14 II II NNP cord-104269-9r7rqqqk 141 15 camera camera NN cord-104269-9r7rqqqk 141 16 system system NN cord-104269-9r7rqqqk 141 17 and and CC cord-104269-9r7rqqqk 141 18 a a DT cord-104269-9r7rqqqk 141 19 dual dual JJ cord-104269-9r7rqqqk 141 20 wavelength wavelength NN cord-104269-9r7rqqqk 141 21 ( ( -LRB- cord-104269-9r7rqqqk 141 22 FiTC FiTC NNP cord-104269-9r7rqqqk 141 23 / / SYM cord-104269-9r7rqqqk 141 24 Texas Texas NNP cord-104269-9r7rqqqk 141 25 red red NN cord-104269-9r7rqqqk 141 26 ) ) -RRB- cord-104269-9r7rqqqk 141 27 epifluorescence epifluorescence NNP cord-104269-9r7rqqqk 141 28 filter filter NN cord-104269-9r7rqqqk 141 29 module module NN cord-104269-9r7rqqqk 141 30 . . . cord-104269-9r7rqqqk 142 1 Micrographs Micrographs NNP cord-104269-9r7rqqqk 142 2 were be VBD cord-104269-9r7rqqqk 142 3 prepared prepare VBN cord-104269-9r7rqqqk 142 4 with with IN cord-104269-9r7rqqqk 142 5 Ektachrome Ektachrome NNP cord-104269-9r7rqqqk 142 6 Pl600 Pl600 NNP cord-104269-9r7rqqqk 142 7 Professional professional JJ cord-104269-9r7rqqqk 142 8 film film NN cord-104269-9r7rqqqk 142 9 processed process VBN cord-104269-9r7rqqqk 142 10 at at IN cord-104269-9r7rqqqk 142 11 ASA ASA NNP cord-104269-9r7rqqqk 142 12 800 800 CD cord-104269-9r7rqqqk 142 13 . . . cord-104269-9r7rqqqk 143 1 Colloidal colloidal JJ cord-104269-9r7rqqqk 143 2 gold gold NN cord-104269-9r7rqqqk 143 3 ( ( -LRB- cord-104269-9r7rqqqk 143 4 6 6 CD cord-104269-9r7rqqqk 143 5 and and CC cord-104269-9r7rqqqk 143 6 10 10 CD cord-104269-9r7rqqqk 143 7 tun tun NNP cord-104269-9r7rqqqk 143 8 diameter diameter NN cord-104269-9r7rqqqk 143 9 ) ) -RRB- cord-104269-9r7rqqqk 143 10 sols sol NNS cord-104269-9r7rqqqk 143 11 were be VBD cord-104269-9r7rqqqk 143 12 made make VBN cord-104269-9r7rqqqk 143 13 as as IN cord-104269-9r7rqqqk 143 14 described describe VBN cord-104269-9r7rqqqk 143 15 by by IN cord-104269-9r7rqqqk 143 16 Slot slot NN cord-104269-9r7rqqqk 144 1 and and CC cord-104269-9r7rqqqk 144 2 ~ ~ NFP cord-104269-9r7rqqqk 144 3 ( ( -LRB- cord-104269-9r7rqqqk 144 4 1985 1985 CD cord-104269-9r7rqqqk 144 5 ) ) -RRB- cord-104269-9r7rqqqk 144 6 . . . cord-104269-9r7rqqqk 145 1 Protein Protein NNP cord-104269-9r7rqqqk 145 2 A a DT cord-104269-9r7rqqqk 145 3 gold gold NN cord-104269-9r7rqqqk 145 4 and and CC cord-104269-9r7rqqqk 145 5 rabbit rabbit NN cord-104269-9r7rqqqk 145 6 anti anti JJ cord-104269-9r7rqqqk 145 7 - - JJ cord-104269-9r7rqqqk 145 8 mouse mouse JJ cord-104269-9r7rqqqk 145 9 Ig Ig NNP cord-104269-9r7rqqqk 145 10 gold gold NN cord-104269-9r7rqqqk 145 11 antibody antibody NN cord-104269-9r7rqqqk 145 12 conjugates conjugate NNS cord-104269-9r7rqqqk 145 13 ( ( -LRB- cord-104269-9r7rqqqk 145 14 1978 1978 CD cord-104269-9r7rqqqk 145 15 ) ) -RRB- cord-104269-9r7rqqqk 145 16 were be VBD cord-104269-9r7rqqqk 145 17 made make VBN cord-104269-9r7rqqqk 145 18 by by IN cord-104269-9r7rqqqk 145 19 standard standard JJ cord-104269-9r7rqqqk 145 20 methods method NNS cord-104269-9r7rqqqk 145 21 ( ( -LRB- cord-104269-9r7rqqqk 145 22 de de FW cord-104269-9r7rqqqk 145 23 Mey Mey NNP cord-104269-9r7rqqqk 145 24 , , , cord-104269-9r7rqqqk 145 25 1986 1986 CD cord-104269-9r7rqqqk 145 26 ) ) -RRB- cord-104269-9r7rqqqk 145 27 and and CC cord-104269-9r7rqqqk 145 28 cryosections cryosection NNS cord-104269-9r7rqqqk 145 29 made make VBN cord-104269-9r7rqqqk 145 30 and and CC cord-104269-9r7rqqqk 145 31 immunolabeled immunolabele VBN cord-104269-9r7rqqqk 145 32 essentially essentially RB cord-104269-9r7rqqqk 145 33 as as IN cord-104269-9r7rqqqk 145 34 described describe VBN cord-104269-9r7rqqqk 145 35 by by IN cord-104269-9r7rqqqk 145 36 Tokuyasu Tokuyasu NNP cord-104269-9r7rqqqk 145 37 ( ( -LRB- cord-104269-9r7rqqqk 145 38 1978 1978 CD cord-104269-9r7rqqqk 145 39 ) ) -RRB- cord-104269-9r7rqqqk 145 40 . . . cord-104269-9r7rqqqk 146 1 The the DT cord-104269-9r7rqqqk 146 2 sections section NNS cord-104269-9r7rqqqk 146 3 were be VBD cord-104269-9r7rqqqk 146 4 incubated incubate VBN cord-104269-9r7rqqqk 146 5 30 30 CD cord-104269-9r7rqqqk 146 6 rain rain NN cord-104269-9r7rqqqk 146 7 with with IN cord-104269-9r7rqqqk 146 8 a a DT cord-104269-9r7rqqqk 146 9 rabbit rabbit NN cord-104269-9r7rqqqk 146 10 antiserum antiserum NN cord-104269-9r7rqqqk 146 11 a a DT cord-104269-9r7rqqqk 146 12 vaingt vaingt NN cord-104269-9r7rqqqk 146 13 human human JJ cord-104269-9r7rqqqk 146 14 TR tr NN cord-104269-9r7rqqqk 146 15 ( ( -LRB- cord-104269-9r7rqqqk 146 16 Trowbridge Trowbridge NNP cord-104269-9r7rqqqk 146 17 , , , cord-104269-9r7rqqqk 146 18 I.S. I.S. NNP cord-104269-9r7rqqqk 146 19 , , , cord-104269-9r7rqqqk 146 20 unpublished unpublished JJ cord-104269-9r7rqqqk 146 21 results result NNS cord-104269-9r7rqqqk 146 22 ) ) -RRB- cord-104269-9r7rqqqk 146 23 followed follow VBN cord-104269-9r7rqqqk 146 24 by by IN cord-104269-9r7rqqqk 146 25 6-am 6-am CD cord-104269-9r7rqqqk 146 26 gold gold NN cord-104269-9r7rqqqk 146 27 protein protein NN cord-104269-9r7rqqqk 146 28 A a NN cord-104269-9r7rqqqk 146 29 for for IN cord-104269-9r7rqqqk 146 30 20 20 CD cord-104269-9r7rqqqk 146 31 rain rain NN cord-104269-9r7rqqqk 146 32 , , , cord-104269-9r7rqqqk 146 33 and and CC cord-104269-9r7rqqqk 146 34 then then RB cord-104269-9r7rqqqk 146 35 rinsed rinse VBD cord-104269-9r7rqqqk 146 36 for for IN cord-104269-9r7rqqqk 146 37 20 20 CD cord-104269-9r7rqqqk 146 38 rain rain NN cord-104269-9r7rqqqk 146 39 with with IN cord-104269-9r7rqqqk 146 40 five five CD cord-104269-9r7rqqqk 146 41 changes change NNS cord-104269-9r7rqqqk 146 42 of of IN cord-104269-9r7rqqqk 146 43 PBS PBS NNP cord-104269-9r7rqqqk 146 44 and and CC cord-104269-9r7rqqqk 146 45 incubated incubate VBN cord-104269-9r7rqqqk 146 46 with with IN cord-104269-9r7rqqqk 146 47 anti anti JJ cord-104269-9r7rqqqk 146 48 - - JJ cord-104269-9r7rqqqk 146 49 LEP LEP NNP cord-104269-9r7rqqqk 146 50 100 100 CD cord-104269-9r7rqqqk 146 51 mouse mouse NN cord-104269-9r7rqqqk 146 52 monoclooal monoclooal NNP cord-104269-9r7rqqqk 146 53 antibody antibody NN cord-104269-9r7rqqqk 146 54 for for IN cord-104269-9r7rqqqk 146 55 30 30 CD cord-104269-9r7rqqqk 146 56 rain rain NN cord-104269-9r7rqqqk 146 57 followed follow VBN cord-104269-9r7rqqqk 146 58 by by IN cord-104269-9r7rqqqk 146 59 10-nm 10-nm CD cord-104269-9r7rqqqk 146 60 gold gold NN cord-104269-9r7rqqqk 146 61 rabbit rabbit NN cord-104269-9r7rqqqk 146 62 anti anti JJ cord-104269-9r7rqqqk 146 63 - - JJ cord-104269-9r7rqqqk 146 64 mouse mouse JJ cord-104269-9r7rqqqk 146 65 Ig Ig NNP cord-104269-9r7rqqqk 146 66 antibody antibody NN cord-104269-9r7rqqqk 146 67 for for IN cord-104269-9r7rqqqk 146 68 20 20 CD cord-104269-9r7rqqqk 146 69 rain rain NN cord-104269-9r7rqqqk 146 70 before before IN cord-104269-9r7rqqqk 146 71 an an DT cord-104269-9r7rqqqk 146 72 additional additional JJ cord-104269-9r7rqqqk 146 73 five five CD cord-104269-9r7rqqqk 146 74 rinses rinse NNS cord-104269-9r7rqqqk 146 75 in in IN cord-104269-9r7rqqqk 146 76 PBS PBS NNP cord-104269-9r7rqqqk 146 77 . . . cord-104269-9r7rqqqk 147 1 Omitting omit VBG cord-104269-9r7rqqqk 147 2 either either CC cord-104269-9r7rqqqk 147 3 first first JJ cord-104269-9r7rqqqk 147 4 or or CC cord-104269-9r7rqqqk 147 5 second second JJ cord-104269-9r7rqqqk 147 6 specific specific JJ cord-104269-9r7rqqqk 147 7 antibodies antibody NNS cord-104269-9r7rqqqk 147 8 indicated indicate VBD cord-104269-9r7rqqqk 147 9 nonspecific nonspecific JJ cord-104269-9r7rqqqk 147 10 labeling labeling NN cord-104269-9r7rqqqk 147 11 was be VBD cord-104269-9r7rqqqk 147 12 less less JJR cord-104269-9r7rqqqk 147 13 than than IN cord-104269-9r7rqqqk 147 14 5 5 CD cord-104269-9r7rqqqk 147 15 % % NN cord-104269-9r7rqqqk 147 16 . . . cord-104269-9r7rqqqk 148 1 Staining stain VBG cord-104269-9r7rqqqk 148 2 and and CC cord-104269-9r7rqqqk 148 3 embedding embedding NN cord-104269-9r7rqqqk 148 4 were be VBD cord-104269-9r7rqqqk 148 5 carried carry VBN cord-104269-9r7rqqqk 148 6 out out RP cord-104269-9r7rqqqk 148 7 as as IN cord-104269-9r7rqqqk 148 8 described describe VBN cord-104269-9r7rqqqk 148 9 previously previously RB cord-104269-9r7rqqqk 148 10 ( ( -LRB- cord-104269-9r7rqqqk 148 11 Hopkim Hopkim NNP cord-104269-9r7rqqqk 148 12 , , , cord-104269-9r7rqqqk 148 13 1983 1983 CD cord-104269-9r7rqqqk 148 14 ) ) -RRB- cord-104269-9r7rqqqk 148 15 and and CC cord-104269-9r7rqqqk 148 16 the the DT cord-104269-9r7rqqqk 148 17 sections section NNS cord-104269-9r7rqqqk 148 18 were be VBD cord-104269-9r7rqqqk 148 19 examined examine VBN cord-104269-9r7rqqqk 148 20 at at IN cord-104269-9r7rqqqk 148 21 80 80 CD cord-104269-9r7rqqqk 148 22 keV keV NNP cord-104269-9r7rqqqk 148 23 in in IN cord-104269-9r7rqqqk 148 24 a a DT cord-104269-9r7rqqqk 148 25 Philips Philips NNP cord-104269-9r7rqqqk 148 26 301 301 CD cord-104269-9r7rqqqk 148 27 electron electron NN cord-104269-9r7rqqqk 148 28 microscope microscope NN cord-104269-9r7rqqqk 148 29 . . . cord-104269-9r7rqqqk 149 1 Quantitation quantitation NN cord-104269-9r7rqqqk 149 2 of of IN cord-104269-9r7rqqqk 149 3 the the DT cord-104269-9r7rqqqk 149 4 Iicr+m Iicr+m NNP cord-104269-9r7rqqqk 149 5 chimera chimera NN cord-104269-9r7rqqqk 149 6 in in IN cord-104269-9r7rqqqk 149 7 clathrin clathrin NN cord-104269-9r7rqqqk 149 8 - - HYPH cord-104269-9r7rqqqk 149 9 coated coat VBN cord-104269-9r7rqqqk 149 10 pits pit NNS cord-104269-9r7rqqqk 149 11 was be VBD cord-104269-9r7rqqqk 149 12 determined determine VBN cord-104269-9r7rqqqk 149 13 on on IN cord-104269-9r7rqqqk 149 14 conventional conventional JJ cord-104269-9r7rqqqk 149 15 plastic plastic JJ cord-104269-9r7rqqqk 149 16 sections section NNS cord-104269-9r7rqqqk 149 17 from from IN cord-104269-9r7rqqqk 149 18 cells cell NNS cord-104269-9r7rqqqk 149 19 incubated incubate VBN cord-104269-9r7rqqqk 149 20 with with IN cord-104269-9r7rqqqk 149 21 B3/25-gold B3/25-gold NNP cord-104269-9r7rqqqk 149 22 complexes complex NNS cord-104269-9r7rqqqk 149 23 at at IN cord-104269-9r7rqqqk 149 24 4 4 CD cord-104269-9r7rqqqk 149 25 ° ° NN cord-104269-9r7rqqqk 149 26 C C NNP cord-104269-9r7rqqqk 149 27 as as IN cord-104269-9r7rqqqk 149 28 described describe VBN cord-104269-9r7rqqqk 149 29 earlier early RBR cord-104269-9r7rqqqk 149 30 ( ( -LRB- cord-104269-9r7rqqqk 149 31 Miller Miller NNP cord-104269-9r7rqqqk 149 32 et et NNP cord-104269-9r7rqqqk 149 33 al al NNP cord-104269-9r7rqqqk 149 34 . . NNP cord-104269-9r7rqqqk 149 35 , , , cord-104269-9r7rqqqk 149 36 1991 1991 CD cord-104269-9r7rqqqk 149 37 ) ) -RRB- cord-104269-9r7rqqqk 149 38 . . . cord-104269-9r7rqqqk 150 1 To to TO cord-104269-9r7rqqqk 150 2 investigate investigate VB cord-104269-9r7rqqqk 150 3 the the DT cord-104269-9r7rqqqk 150 4 signal signal NN cord-104269-9r7rqqqk 150 5 - - HYPH cord-104269-9r7rqqqk 150 6 dependent dependent JJ cord-104269-9r7rqqqk 150 7 trafficking trafficking NN cord-104269-9r7rqqqk 150 8 of of IN cord-104269-9r7rqqqk 150 9 fi fi NNP cord-104269-9r7rqqqk 150 10 , , , cord-104269-9r7rqqqk 150 11 we -PRON- PRP cord-104269-9r7rqqqk 150 12 constructed construct VBD cord-104269-9r7rqqqk 150 13 Ii Ii NNP cord-104269-9r7rqqqk 150 14 - - HYPH cord-104269-9r7rqqqk 150 15 TR TR NNP cord-104269-9r7rqqqk 150 16 chimeras chimera NNS cord-104269-9r7rqqqk 150 17 consisting consist VBG cord-104269-9r7rqqqk 150 18 of of IN cord-104269-9r7rqqqk 150 19 the the DT cord-104269-9r7rqqqk 150 20 fi fi NN cord-104269-9r7rqqqk 150 21 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 150 22 tail tail NN cord-104269-9r7rqqqk 150 23 , , , cord-104269-9r7rqqqk 150 24 the the DT cord-104269-9r7rqqqk 150 25 TR tr NN cord-104269-9r7rqqqk 150 26 extracellular extracellular JJ cord-104269-9r7rqqqk 150 27 domain domain NN cord-104269-9r7rqqqk 150 28 , , , cord-104269-9r7rqqqk 150 29 and and CC cord-104269-9r7rqqqk 150 30 the the DT cord-104269-9r7rqqqk 150 31 transmembrane transmembrane NN cord-104269-9r7rqqqk 150 32 region region NN cord-104269-9r7rqqqk 150 33 from from IN cord-104269-9r7rqqqk 150 34 either either DT cord-104269-9r7rqqqk 150 35 TR TR NNP cord-104269-9r7rqqqk 150 36 or or CC cord-104269-9r7rqqqk 150 37 from from IN cord-104269-9r7rqqqk 150 38 Ii Ii NNP cord-104269-9r7rqqqk 150 39 ( ( -LRB- cord-104269-9r7rqqqk 150 40 Fig Fig NNP cord-104269-9r7rqqqk 150 41 . . . cord-104269-9r7rqqqk 150 42 1 1 CD cord-104269-9r7rqqqk 150 43 ) ) -RRB- cord-104269-9r7rqqqk 150 44 . . . cord-104269-9r7rqqqk 151 1 We -PRON- PRP cord-104269-9r7rqqqk 151 2 reasoned reason VBD cord-104269-9r7rqqqk 151 3 that that IN cord-104269-9r7rqqqk 151 4 fi fi NN cord-104269-9r7rqqqk 151 5 - - HYPH cord-104269-9r7rqqqk 151 6 TR tr NN cord-104269-9r7rqqqk 151 7 chimeras chimera NNS cord-104269-9r7rqqqk 151 8 would would MD cord-104269-9r7rqqqk 151 9 be be VB cord-104269-9r7rqqqk 151 10 expressed express VBN cord-104269-9r7rqqqk 151 11 in in IN cord-104269-9r7rqqqk 151 12 sufficient sufficient JJ cord-104269-9r7rqqqk 151 13 amounts amount NNS cord-104269-9r7rqqqk 151 14 on on IN cord-104269-9r7rqqqk 151 15 the the DT cord-104269-9r7rqqqk 151 16 cell cell NN cord-104269-9r7rqqqk 151 17 surface surface NN cord-104269-9r7rqqqk 151 18 to to TO cord-104269-9r7rqqqk 151 19 identify identify VB cord-104269-9r7rqqqk 151 20 Ii Ii NNP cord-104269-9r7rqqqk 151 21 sorting sorting NN cord-104269-9r7rqqqk 151 22 signals signal NNS cord-104269-9r7rqqqk 151 23 using use VBG cord-104269-9r7rqqqk 151 24 quantitative quantitative JJ cord-104269-9r7rqqqk 151 25 assays assay NNS cord-104269-9r7rqqqk 151 26 which which WDT cord-104269-9r7rqqqk 151 27 measure measure VBP cord-104269-9r7rqqqk 151 28 Tf Tf NNP cord-104269-9r7rqqqk 151 29 internalization internalization NN cord-104269-9r7rqqqk 151 30 and and CC cord-104269-9r7rqqqk 151 31 iron iron NN cord-104269-9r7rqqqk 151 32 uptake uptake NN cord-104269-9r7rqqqk 151 33 . . . cord-104269-9r7rqqqk 152 1 As as IN cord-104269-9r7rqqqk 152 2 there there EX cord-104269-9r7rqqqk 152 3 is be VBZ cord-104269-9r7rqqqk 152 4 increasing increase VBG cord-104269-9r7rqqqk 152 5 evidence evidence NN cord-104269-9r7rqqqk 152 6 that that IN cord-104269-9r7rqqqk 152 7 the the DT cord-104269-9r7rqqqk 152 8 same same JJ cord-104269-9r7rqqqk 152 9 or or CC cord-104269-9r7rqqqk 152 10 closely closely RB cord-104269-9r7rqqqk 152 11 related related JJ cord-104269-9r7rqqqk 152 12 signals signal NNS cord-104269-9r7rqqqk 152 13 are be VBP cord-104269-9r7rqqqk 152 14 recognized recognize VBN cord-104269-9r7rqqqk 152 15 by by IN cord-104269-9r7rqqqk 152 16 the the DT cord-104269-9r7rqqqk 152 17 clathrin clathrin NN cord-104269-9r7rqqqk 152 18 - - HYPH cord-104269-9r7rqqqk 152 19 based base VBN cord-104269-9r7rqqqk 152 20 sorting sorting NN cord-104269-9r7rqqqk 152 21 machinery machinery NN cord-104269-9r7rqqqk 152 22 at at IN cord-104269-9r7rqqqk 152 23 the the DT cord-104269-9r7rqqqk 152 24 plasma plasma NN cord-104269-9r7rqqqk 152 25 membrane membrane NN cord-104269-9r7rqqqk 152 26 and and CC cord-104269-9r7rqqqk 152 27 trans trans NNP cord-104269-9r7rqqqk 152 28 - - NNP cord-104269-9r7rqqqk 152 29 Golgi Golgi NNP cord-104269-9r7rqqqk 152 30 ( ( -LRB- cord-104269-9r7rqqqk 152 31 Letourneur Letourneur NNP cord-104269-9r7rqqqk 152 32 and and CC cord-104269-9r7rqqqk 152 33 Klausner Klausner NNP cord-104269-9r7rqqqk 152 34 , , , cord-104269-9r7rqqqk 152 35 1992 1992 CD cord-104269-9r7rqqqk 152 36 would would MD cord-104269-9r7rqqqk 152 37 also also RB cord-104269-9r7rqqqk 152 38 allow allow VB cord-104269-9r7rqqqk 152 39 identification identification NN cord-104269-9r7rqqqk 152 40 of of IN cord-104269-9r7rqqqk 152 41 Ii Ii NNP cord-104269-9r7rqqqk 152 42 sorting sorting NN cord-104269-9r7rqqqk 152 43 signals signal NNS cord-104269-9r7rqqqk 152 44 operative operative JJ cord-104269-9r7rqqqk 152 45 along along IN cord-104269-9r7rqqqk 152 46 the the DT cord-104269-9r7rqqqk 152 47 intracelhlar intracelhlar JJ cord-104269-9r7rqqqk 152 48 trafficking traffic VBG cord-104269-9r7rqqqk 152 49 pathway pathway NN cord-104269-9r7rqqqk 152 50 . . . cord-104269-9r7rqqqk 153 1 Wild wild JJ cord-104269-9r7rqqqk 153 2 - - HYPH cord-104269-9r7rqqqk 153 3 type type NN cord-104269-9r7rqqqk 153 4 human human NN cord-104269-9r7rqqqk 153 5 TR tr NN cord-104269-9r7rqqqk 153 6 and and CC cord-104269-9r7rqqqk 153 7 Ii Ii NNP cord-104269-9r7rqqqk 153 8 - - HYPH cord-104269-9r7rqqqk 153 9 TR TR NNP cord-104269-9r7rqqqk 153 10 chimeras chimera NNS cord-104269-9r7rqqqk 153 11 containing contain VBG cord-104269-9r7rqqqk 154 1 either either CC cord-104269-9r7rqqqk 154 2 the the DT cord-104269-9r7rqqqk 154 3 Iicr Iicr NNP cord-104269-9r7rqqqk 154 4 or or CC cord-104269-9r7rqqqk 154 5 Iicr÷ru Iicr÷ru NNP cord-104269-9r7rqqqk 154 6 were be VBD cord-104269-9r7rqqqk 154 7 stably stably RB cord-104269-9r7rqqqk 154 8 expressed express VBN cord-104269-9r7rqqqk 154 9 in in IN cord-104269-9r7rqqqk 154 10 CEF CEF NNP cord-104269-9r7rqqqk 154 11 using use VBG cord-104269-9r7rqqqk 154 12 BH BH NNP cord-104269-9r7rqqqk 154 13 - - HYPH cord-104269-9r7rqqqk 154 14 RCAS RCAS NNP cord-104269-9r7rqqqk 154 15 , , , cord-104269-9r7rqqqk 154 16 a a DT cord-104269-9r7rqqqk 154 17 replication replication NN cord-104269-9r7rqqqk 154 18 - - HYPH cord-104269-9r7rqqqk 154 19 competent competent JJ cord-104269-9r7rqqqk 154 20 retroviral retroviral NN cord-104269-9r7rqqqk 154 21 vector vector NN cord-104269-9r7rqqqk 154 22 derived derive VBN cord-104269-9r7rqqqk 154 23 from from IN cord-104269-9r7rqqqk 154 24 Rous rous JJ cord-104269-9r7rqqqk 154 25 sarcoma sarcoma NN cord-104269-9r7rqqqk 154 26 virus virus NN cord-104269-9r7rqqqk 154 27 Hughes Hughes NNP cord-104269-9r7rqqqk 154 28 et et NNP cord-104269-9r7rqqqk 154 29 al al NNP cord-104269-9r7rqqqk 154 30 . . NNP cord-104269-9r7rqqqk 154 31 , , , cord-104269-9r7rqqqk 154 32 1990 1990 CD cord-104269-9r7rqqqk 154 33 ) ) -RRB- cord-104269-9r7rqqqk 154 34 . . . cord-104269-9r7rqqqk 155 1 Binding binding NN cord-104269-9r7rqqqk 155 2 studies study NNS cord-104269-9r7rqqqk 155 3 at at IN cord-104269-9r7rqqqk 155 4 4 4 CD cord-104269-9r7rqqqk 155 5 ° ° NN cord-104269-9r7rqqqk 155 6 C c NN cord-104269-9r7rqqqk 155 7 using use VBG cord-104269-9r7rqqqk 155 8 ~2SI ~2si ADD cord-104269-9r7rqqqk 155 9 - - HYPH cord-104269-9r7rqqqk 155 10 labeled label VBN cord-104269-9r7rqqqk 155 11 human human NN cord-104269-9r7rqqqk 155 12 Tf Tf NNP cord-104269-9r7rqqqk 155 13 indicated indicate VBD cord-104269-9r7rqqqk 155 14 that that IN cord-104269-9r7rqqqk 155 15 both both DT cord-104269-9r7rqqqk 155 16 Ii Ii NNP cord-104269-9r7rqqqk 155 17 - - HYPH cord-104269-9r7rqqqk 155 18 TR TR NNP cord-104269-9r7rqqqk 155 19 chimeras chimera NNS cord-104269-9r7rqqqk 155 20 were be VBD cord-104269-9r7rqqqk 155 21 expressed express VBN cord-104269-9r7rqqqk 155 22 on on IN cord-104269-9r7rqqqk 155 23 the the DT cord-104269-9r7rqqqk 155 24 cell cell NN cord-104269-9r7rqqqk 155 25 surface surface NN cord-104269-9r7rqqqk 155 26 of of IN cord-104269-9r7rqqqk 155 27 infected infected JJ cord-104269-9r7rqqqk 155 28 CEF CEF NNP cord-104269-9r7rqqqk 155 29 , , , cord-104269-9r7rqqqk 155 30 but but CC cord-104269-9r7rqqqk 155 31 at at IN cord-104269-9r7rqqqk 155 32 lower low JJR cord-104269-9r7rqqqk 155 33 levels level NNS cord-104269-9r7rqqqk 155 34 than than IN cord-104269-9r7rqqqk 155 35 wild wild JJ cord-104269-9r7rqqqk 155 36 - - HYPH cord-104269-9r7rqqqk 155 37 type type NN cord-104269-9r7rqqqk 155 38 TR tr NN cord-104269-9r7rqqqk 155 39 ( ( -LRB- cord-104269-9r7rqqqk 156 1 51 51 CD cord-104269-9r7rqqqk 156 2 + + SYM cord-104269-9r7rqqqk 156 3 13 13 CD cord-104269-9r7rqqqk 156 4 % % NN cord-104269-9r7rqqqk 156 5 Iicr Iicr NNP cord-104269-9r7rqqqk 156 6 and and CC cord-104269-9r7rqqqk 156 7 12 12 CD cord-104269-9r7rqqqk 156 8 ± ± CD cord-104269-9r7rqqqk 156 9 1 1 CD cord-104269-9r7rqqqk 156 10 % % NN cord-104269-9r7rqqqk 156 11 Iicr÷rM iicr÷rm NN cord-104269-9r7rqqqk 156 12 relative relative JJ cord-104269-9r7rqqqk 156 13 to to IN cord-104269-9r7rqqqk 156 14 wildtype wildtype JJ cord-104269-9r7rqqqk 156 15 TR tr NN cord-104269-9r7rqqqk 156 16 ) ) -RRB- cord-104269-9r7rqqqk 156 17 . . . cord-104269-9r7rqqqk 157 1 Internalization internalization NN cord-104269-9r7rqqqk 157 2 of of IN cord-104269-9r7rqqqk 157 3 the the DT cord-104269-9r7rqqqk 157 4 Ii Ii NNP cord-104269-9r7rqqqk 157 5 - - HYPH cord-104269-9r7rqqqk 157 6 TR TR NNP cord-104269-9r7rqqqk 157 7 chimeras chimera NNS cord-104269-9r7rqqqk 157 8 was be VBD cord-104269-9r7rqqqk 157 9 assayed assay VBN cord-104269-9r7rqqqk 157 10 by by IN cord-104269-9r7rqqqk 157 11 measuring measure VBG cord-104269-9r7rqqqk 157 12 the the DT cord-104269-9r7rqqqk 157 13 steady steady JJ cord-104269-9r7rqqqk 157 14 - - HYPH cord-104269-9r7rqqqk 157 15 state state NN cord-104269-9r7rqqqk 157 16 distribution distribution NN cord-104269-9r7rqqqk 157 17 of of IN cord-104269-9r7rqqqk 157 18 internalized internalize VBN cord-104269-9r7rqqqk 157 19 receptors receptor NNS cord-104269-9r7rqqqk 157 20 and and CC cord-104269-9r7rqqqk 157 21 their -PRON- PRP$ cord-104269-9r7rqqqk 157 22 ability ability NN cord-104269-9r7rqqqk 157 23 to to TO cord-104269-9r7rqqqk 157 24 mediate mediate VB cord-104269-9r7rqqqk 157 25 iron iron NN cord-104269-9r7rqqqk 157 26 uptake uptake NN cord-104269-9r7rqqqk 157 27 Collawn Collawn NNP cord-104269-9r7rqqqk 157 28 et et NNP cord-104269-9r7rqqqk 157 29 al al NNP cord-104269-9r7rqqqk 157 30 . . NNP cord-104269-9r7rqqqk 157 31 , , , cord-104269-9r7rqqqk 157 32 1990 1990 CD cord-104269-9r7rqqqk 157 33 ) ) -RRB- cord-104269-9r7rqqqk 157 34 . . . cord-104269-9r7rqqqk 158 1 The the DT cord-104269-9r7rqqqk 158 2 Iicr Iicr NNP cord-104269-9r7rqqqk 158 3 chimera chimera NN cord-104269-9r7rqqqk 158 4 was be VBD cord-104269-9r7rqqqk 158 5 efficiently efficiently RB cord-104269-9r7rqqqk 158 6 internalized internalize VBN cord-104269-9r7rqqqk 158 7 , , , cord-104269-9r7rqqqk 158 8 as as IN cord-104269-9r7rqqqk 158 9 judged judge VBN cord-104269-9r7rqqqk 158 10 by by IN cord-104269-9r7rqqqk 158 11 either either DT cord-104269-9r7rqqqk 158 12 iron iron NN cord-104269-9r7rqqqk 158 13 uptake uptake NN cord-104269-9r7rqqqk 158 14 or or CC cord-104269-9r7rqqqk 158 15 steady steady JJ cord-104269-9r7rqqqk 158 16 - - HYPH cord-104269-9r7rqqqk 158 17 state state NN cord-104269-9r7rqqqk 158 18 assays assay NNS cord-104269-9r7rqqqk 158 19 ( ( -LRB- cord-104269-9r7rqqqk 158 20 Table table NN cord-104269-9r7rqqqk 158 21 I -PRON- PRP cord-104269-9r7rqqqk 158 22 ) ) -RRB- cord-104269-9r7rqqqk 158 23 , , , cord-104269-9r7rqqqk 158 24 indicating indicate VBG cord-104269-9r7rqqqk 158 25 that that IN cord-104269-9r7rqqqk 158 26 the the DT cord-104269-9r7rqqqk 158 27 Ii Ii NNP cord-104269-9r7rqqqk 158 28 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 158 29 domain domain NN cord-104269-9r7rqqqk 158 30 contains contain VBZ cord-104269-9r7rqqqk 158 31 internalization internalization NN cord-104269-9r7rqqqk 158 32 signal(s signal(s XX cord-104269-9r7rqqqk 158 33 ) ) -RRB- cord-104269-9r7rqqqk 158 34 that that WDT cord-104269-9r7rqqqk 158 35 can can MD cord-104269-9r7rqqqk 158 36 promote promote VB cord-104269-9r7rqqqk 158 37 rapid rapid JJ cord-104269-9r7rqqqk 158 38 endocytosis endocytosis NN cord-104269-9r7rqqqk 158 39 of of IN cord-104269-9r7rqqqk 158 40 the the DT cord-104269-9r7rqqqk 158 41 human human JJ cord-104269-9r7rqqqk 158 42 TR tr NN cord-104269-9r7rqqqk 158 43 . . . cord-104269-9r7rqqqk 159 1 Electron electron NN cord-104269-9r7rqqqk 159 2 microscopy microscopy NN cord-104269-9r7rqqqk 159 3 ( ( -LRB- cord-104269-9r7rqqqk 159 4 see see VB cord-104269-9r7rqqqk 159 5 Fig Fig NNP cord-104269-9r7rqqqk 159 6 . . . cord-104269-9r7rqqqk 159 7 _SP cord-104269-9r7rqqqk 159 8 6 6 CD cord-104269-9r7rqqqk 159 9 , , , cord-104269-9r7rqqqk 159 10 inset inset NN cord-104269-9r7rqqqk 159 11 ) ) -RRB- cord-104269-9r7rqqqk 159 12 clearly clearly RB cord-104269-9r7rqqqk 159 13 demonstrates demonstrate VBZ cord-104269-9r7rqqqk 159 14 that that IN cord-104269-9r7rqqqk 159 15 Ii Ii NNP cord-104269-9r7rqqqk 159 16 - - HYPH cord-104269-9r7rqqqk 159 17 TR TR NNP cord-104269-9r7rqqqk 159 18 chimeras chimera NNS cord-104269-9r7rqqqk 159 19 internalize internalize VBP cord-104269-9r7rqqqk 159 20 via via IN cord-104269-9r7rqqqk 159 21 clathrin clathrin NN cord-104269-9r7rqqqk 159 22 - - HYPH cord-104269-9r7rqqqk 159 23 coated coat VBN cord-104269-9r7rqqqk 159 24 pits pit NNS cord-104269-9r7rqqqk 159 25 , , , cord-104269-9r7rqqqk 159 26 15 15 CD cord-104269-9r7rqqqk 159 27 % % NN cord-104269-9r7rqqqk 159 28 of of IN cord-104269-9r7rqqqk 159 29 the the DT cord-104269-9r7rqqqk 159 30 label label NN cord-104269-9r7rqqqk 159 31 on on IN cord-104269-9r7rqqqk 159 32 the the DT cord-104269-9r7rqqqk 159 33 plasma plasma NN cord-104269-9r7rqqqk 159 34 membrane membrane NN cord-104269-9r7rqqqk 159 35 being be VBG cord-104269-9r7rqqqk 159 36 located locate VBN cord-104269-9r7rqqqk 159 37 within within IN cord-104269-9r7rqqqk 159 38 these these DT cord-104269-9r7rqqqk 159 39 domains domain NNS cord-104269-9r7rqqqk 159 40 . . . cord-104269-9r7rqqqk 160 1 The the DT cord-104269-9r7rqqqk 160 2 apparent apparent JJ cord-104269-9r7rqqqk 160 3 internalization internalization NN cord-104269-9r7rqqqk 160 4 efficiency efficiency NN cord-104269-9r7rqqqk 160 5 of of IN cord-104269-9r7rqqqk 160 6 the the DT cord-104269-9r7rqqqk 160 7 Iicr+ru iicr+ru CD cord-104269-9r7rqqqk 160 8 chimera chimera NN cord-104269-9r7rqqqk 160 9 was be VBD cord-104269-9r7rqqqk 160 10 significantly significantly RB cord-104269-9r7rqqqk 160 11 higher high JJR cord-104269-9r7rqqqk 160 12 than than IN cord-104269-9r7rqqqk 160 13 either either DT cord-104269-9r7rqqqk 160 14 wild wild JJ cord-104269-9r7rqqqk 160 15 - - HYPH cord-104269-9r7rqqqk 160 16 type type NN cord-104269-9r7rqqqk 160 17 TR tr NN cord-104269-9r7rqqqk 160 18 or or CC cord-104269-9r7rqqqk 160 19 the the DT cord-104269-9r7rqqqk 160 20 Iicr Iicr NNP cord-104269-9r7rqqqk 160 21 chimera chimera NN cord-104269-9r7rqqqk 160 22 , , , cord-104269-9r7rqqqk 160 23 implying imply VBG cord-104269-9r7rqqqk 160 24 that that IN cord-104269-9r7rqqqk 160 25 the the DT cord-104269-9r7rqqqk 160 26 transmembrane transmembrane NNP cord-104269-9r7rqqqk 160 27 region region NN cord-104269-9r7rqqqk 160 28 of of IN cord-104269-9r7rqqqk 160 29 Ii Ii NNP cord-104269-9r7rqqqk 160 30 also also RB cord-104269-9r7rqqqk 160 31 influences influence VBZ cord-104269-9r7rqqqk 160 32 trafficking traffic VBG cord-104269-9r7rqqqk 160 33 . . . cord-104269-9r7rqqqk 161 1 To to TO cord-104269-9r7rqqqk 161 2 locate locate VB cord-104269-9r7rqqqk 161 3 the the DT cord-104269-9r7rqqqk 161 4 Ii Ii NNP cord-104269-9r7rqqqk 161 5 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 161 6 tail tail NN cord-104269-9r7rqqqk 161 7 internalization internalization NN cord-104269-9r7rqqqk 161 8 signal(s signal(s XX cord-104269-9r7rqqqk 161 9 ) ) -RRB- cord-104269-9r7rqqqk 161 10 , , , cord-104269-9r7rqqqk 161 11 deletion deletion NN cord-104269-9r7rqqqk 161 12 mutants mutant NNS cord-104269-9r7rqqqk 161 13 of of IN cord-104269-9r7rqqqk 161 14 the the DT cord-104269-9r7rqqqk 161 15 Iicr Iicr NNP cord-104269-9r7rqqqk 161 16 chimera chimera NN cord-104269-9r7rqqqk 161 17 were be VBD cord-104269-9r7rqqqk 161 18 constructed construct VBN cord-104269-9r7rqqqk 161 19 ( ( -LRB- cord-104269-9r7rqqqk 161 20 Fig Fig NNP cord-104269-9r7rqqqk 161 21 . . . cord-104269-9r7rqqqk 161 22 _SP cord-104269-9r7rqqqk 161 23 1 1 CD cord-104269-9r7rqqqk 161 24 ) ) -RRB- cord-104269-9r7rqqqk 161 25 . . . cord-104269-9r7rqqqk 162 1 Deletion deletion NN cord-104269-9r7rqqqk 162 2 of of IN cord-104269-9r7rqqqk 162 3 residues residue NNS cord-104269-9r7rqqqk 162 4 2 2 CD cord-104269-9r7rqqqk 162 5 - - SYM cord-104269-9r7rqqqk 162 6 5 5 CD cord-104269-9r7rqqqk 162 7 or or CC cord-104269-9r7rqqqk 162 8 residues residue NNS cord-104269-9r7rqqqk 162 9 2 2 CD cord-104269-9r7rqqqk 162 10 - - SYM cord-104269-9r7rqqqk 162 11 11 11 CD cord-104269-9r7rqqqk 162 12 reduced reduce VBD cord-104269-9r7rqqqk 162 13 the the DT cord-104269-9r7rqqqk 162 14 apparent apparent JJ cord-104269-9r7rqqqk 162 15 internalization internalization NN cord-104269-9r7rqqqk 162 16 efficiency efficiency NN cord-104269-9r7rqqqk 162 17 of of IN cord-104269-9r7rqqqk 162 18 the the DT cord-104269-9r7rqqqk 162 19 Iicr Iicr NNP cord-104269-9r7rqqqk 162 20 chimera chimera NN cord-104269-9r7rqqqk 162 21 to to IN cord-104269-9r7rqqqk 162 22 ~50 ~50 CD cord-104269-9r7rqqqk 162 23 % % NN cord-104269-9r7rqqqk 162 24 , , , cord-104269-9r7rqqqk 162 25 whereas whereas IN cord-104269-9r7rqqqk 162 26 deletion deletion NN cord-104269-9r7rqqqk 162 27 of of IN cord-104269-9r7rqqqk 162 28 residues residue NNS cord-104269-9r7rqqqk 162 29 2 2 CD cord-104269-9r7rqqqk 162 30 - - SYM cord-104269-9r7rqqqk 162 31 17 17 CD cord-104269-9r7rqqqk 162 32 completely completely RB cord-104269-9r7rqqqk 162 33 abolished abolish VBN cord-104269-9r7rqqqk 162 34 rapid rapid JJ cord-104269-9r7rqqqk 162 35 endocytosis endocytosis NN cord-104269-9r7rqqqk 162 36 ( ( -LRB- cord-104269-9r7rqqqk 162 37 Table Table NNP cord-104269-9r7rqqqk 162 38 II II NNP cord-104269-9r7rqqqk 162 39 ) ) -RRB- cord-104269-9r7rqqqk 162 40 . . . cord-104269-9r7rqqqk 163 1 These these DT cord-104269-9r7rqqqk 163 2 results result NNS cord-104269-9r7rqqqk 163 3 suggest suggest VBP cord-104269-9r7rqqqk 163 4 that that IN cord-104269-9r7rqqqk 163 5 the the DT cord-104269-9r7rqqqk 163 6 Ii Ii NNP cord-104269-9r7rqqqk 163 7 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 163 8 tail tail NN cord-104269-9r7rqqqk 163 9 contains contain VBZ cord-104269-9r7rqqqk 163 10 two two CD cord-104269-9r7rqqqk 163 11 internalization internalization NN cord-104269-9r7rqqqk 163 12 signals signal NNS cord-104269-9r7rqqqk 163 13 , , , cord-104269-9r7rqqqk 163 14 one one CD cord-104269-9r7rqqqk 163 15 located locate VBN cord-104269-9r7rqqqk 163 16 between between IN cord-104269-9r7rqqqk 163 17 residues residue NNS cord-104269-9r7rqqqk 163 18 2 2 CD cord-104269-9r7rqqqk 163 19 - - SYM cord-104269-9r7rqqqk 163 20 11 11 CD cord-104269-9r7rqqqk 163 21 , , , cord-104269-9r7rqqqk 163 22 the the DT cord-104269-9r7rqqqk 163 23 other other JJ cord-104269-9r7rqqqk 163 24 between between IN cord-104269-9r7rqqqk 163 25 residues residue NNS cord-104269-9r7rqqqk 163 26 12 12 CD cord-104269-9r7rqqqk 163 27 - - SYM cord-104269-9r7rqqqk 163 28 17 17 CD cord-104269-9r7rqqqk 163 29 . . . cord-104269-9r7rqqqk 164 1 Since since IN cord-104269-9r7rqqqk 164 2 the the DT cord-104269-9r7rqqqk 164 3 Ii Ii NNP cord-104269-9r7rqqqk 164 4 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 164 5 tail tail NN cord-104269-9r7rqqqk 164 6 does do VBZ cord-104269-9r7rqqqk 164 7 not not RB cord-104269-9r7rqqqk 164 8 contain contain VB cord-104269-9r7rqqqk 164 9 aromatic aromatic JJ cord-104269-9r7rqqqk 164 10 residues residue NNS cord-104269-9r7rqqqk 164 11 , , , cord-104269-9r7rqqqk 164 12 the the DT cord-104269-9r7rqqqk 164 13 internalization internalization NN cord-104269-9r7rqqqk 164 14 signals signal NNS cord-104269-9r7rqqqk 164 15 identified identify VBN cord-104269-9r7rqqqk 164 16 by by IN cord-104269-9r7rqqqk 164 17 analysis analysis NN cord-104269-9r7rqqqk 164 18 of of IN cord-104269-9r7rqqqk 164 19 the the DT cord-104269-9r7rqqqk 164 20 Iicr Iicr NNP cord-104269-9r7rqqqk 164 21 deletion deletion NN cord-104269-9r7rqqqk 164 22 mutants mutant NNS cord-104269-9r7rqqqk 164 23 can can MD cord-104269-9r7rqqqk 164 24 not not RB cord-104269-9r7rqqqk 164 25 be be VB cord-104269-9r7rqqqk 164 26 tyrosine tyrosine NN cord-104269-9r7rqqqk 164 27 - - HYPH cord-104269-9r7rqqqk 164 28 based base VBN cord-104269-9r7rqqqk 164 29 . . . cord-104269-9r7rqqqk 165 1 However however RB cord-104269-9r7rqqqk 165 2 , , , cord-104269-9r7rqqqk 165 3 the the DT cord-104269-9r7rqqqk 165 4 Ii Ii NNP cord-104269-9r7rqqqk 165 5 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 165 6 tail tail NN cord-104269-9r7rqqqk 165 7 contains contain VBZ cord-104269-9r7rqqqk 165 8 three three CD cord-104269-9r7rqqqk 165 9 leucine leucine NN cord-104269-9r7rqqqk 165 10 residues residue NNS cord-104269-9r7rqqqk 165 11 ( ( -LRB- cord-104269-9r7rqqqk 165 12 residues residue NNS cord-104269-9r7rqqqk 165 13 7 7 CD cord-104269-9r7rqqqk 165 14 , , , cord-104269-9r7rqqqk 165 15 14 14 CD cord-104269-9r7rqqqk 165 16 , , , cord-104269-9r7rqqqk 165 17 and and CC cord-104269-9r7rqqqk 165 18 17 17 CD cord-104269-9r7rqqqk 165 19 ) ) -RRB- cord-104269-9r7rqqqk 165 20 within within IN cord-104269-9r7rqqqk 165 21 the the DT cord-104269-9r7rqqqk 165 22 first first JJ cord-104269-9r7rqqqk 165 23 17 17 CD cord-104269-9r7rqqqk 165 24 amino amino NN cord-104269-9r7rqqqk 165 25 - - HYPH cord-104269-9r7rqqqk 165 26 terminal terminal NN cord-104269-9r7rqqqk 165 27 residues residue NNS cord-104269-9r7rqqqk 165 28 ( ( -LRB- cord-104269-9r7rqqqk 165 29 Fig Fig NNP cord-104269-9r7rqqqk 165 30 . . . cord-104269-9r7rqqqk 165 31 1 1 CD cord-104269-9r7rqqqk 165 32 ) ) -RRB- cord-104269-9r7rqqqk 165 33 , , , cord-104269-9r7rqqqk 165 34 suggesting suggest VBG cord-104269-9r7rqqqk 165 35 that that IN cord-104269-9r7rqqqk 165 36 internalization internalization NN cord-104269-9r7rqqqk 165 37 of of IN cord-104269-9r7rqqqk 165 38 the the DT cord-104269-9r7rqqqk 165 39 Ii Ii NNP cord-104269-9r7rqqqk 165 40 - - HYPH cord-104269-9r7rqqqk 165 41 TR TR NNP cord-104269-9r7rqqqk 165 42 chimeras chimera NNS cord-104269-9r7rqqqk 165 43 may may MD cord-104269-9r7rqqqk 165 44 be be VB cord-104269-9r7rqqqk 165 45 mediated mediate VBN cord-104269-9r7rqqqk 165 46 by by IN cord-104269-9r7rqqqk 165 47 signals signal NNS cord-104269-9r7rqqqk 165 48 related relate VBN cord-104269-9r7rqqqk 165 49 to to IN cord-104269-9r7rqqqk 165 50 the the DT cord-104269-9r7rqqqk 165 51 dileucine dileucine NN cord-104269-9r7rqqqk 165 52 - - HYPH cord-104269-9r7rqqqk 165 53 based base VBN cord-104269-9r7rqqqk 165 54 sorting sorting NN cord-104269-9r7rqqqk 165 55 signals signal NNS cord-104269-9r7rqqqk 165 56 previously previously RB cord-104269-9r7rqqqk 165 57 identified identify VBN cord-104269-9r7rqqqk 165 58 in in IN cord-104269-9r7rqqqk 165 59 the the DT cord-104269-9r7rqqqk 165 60 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 165 61 tails tail NNS cord-104269-9r7rqqqk 165 62 of of IN cord-104269-9r7rqqqk 165 63 the the DT cord-104269-9r7rqqqk 165 64 T T NNP cord-104269-9r7rqqqk 165 65 cell cell NN cord-104269-9r7rqqqk 165 66 receptor receptor NN cord-104269-9r7rqqqk 165 67 CD3"y cd3"y CD cord-104269-9r7rqqqk 165 68 chain chain NN cord-104269-9r7rqqqk 165 69 and and CC cord-104269-9r7rqqqk 165 70 the the DT cord-104269-9r7rqqqk 165 71 cation cation NN cord-104269-9r7rqqqk 165 72 - - HYPH cord-104269-9r7rqqqk 165 73 independent independent JJ cord-104269-9r7rqqqk 165 74 and and CC cord-104269-9r7rqqqk 165 75 cation cation NN cord-104269-9r7rqqqk 165 76 - - HYPH cord-104269-9r7rqqqk 165 77 dependent dependent JJ cord-104269-9r7rqqqk 165 78 mannose-6-pbosphate mannose-6-pbosphate JJ cord-104269-9r7rqqqk 165 79 receptors receptor NNS cord-104269-9r7rqqqk 165 80 ( ( -LRB- cord-104269-9r7rqqqk 165 81 Letourneur Letourneur NNP cord-104269-9r7rqqqk 165 82 and and CC cord-104269-9r7rqqqk 165 83 Klausner Klausner NNP cord-104269-9r7rqqqk 165 84 , , , cord-104269-9r7rqqqk 165 85 1992 1992 CD cord-104269-9r7rqqqk 165 86 ; ; : cord-104269-9r7rqqqk 165 87 Johnson Johnson NNP cord-104269-9r7rqqqk 165 88 and and CC cord-104269-9r7rqqqk 165 89 Kornfeld Kornfeld NNP cord-104269-9r7rqqqk 165 90 , , , cord-104269-9r7rqqqk 165 91 1992a 1992a CD cord-104269-9r7rqqqk 165 92 , , , cord-104269-9r7rqqqk 165 93 b b LS cord-104269-9r7rqqqk 165 94 ) ) -RRB- cord-104269-9r7rqqqk 165 95 . . . cord-104269-9r7rqqqk 166 1 Although although IN cord-104269-9r7rqqqk 166 2 first first RB cord-104269-9r7rqqqk 166 3 identified identify VBN cord-104269-9r7rqqqk 166 4 as as IN cord-104269-9r7rqqqk 166 5 a a DT cord-104269-9r7rqqqk 166 6 lysosomal lysosomal JJ cord-104269-9r7rqqqk 166 7 targeting target VBG cord-104269-9r7rqqqk 166 8 signal signal NN cord-104269-9r7rqqqk 166 9 , , , cord-104269-9r7rqqqk 166 10 the the DT cord-104269-9r7rqqqk 166 11 CD37 CD37 NNP cord-104269-9r7rqqqk 166 12 chain chain NN cord-104269-9r7rqqqk 166 13 di di NN cord-104269-9r7rqqqk 166 14 - - HYPH cord-104269-9r7rqqqk 166 15 leucine leucine NN cord-104269-9r7rqqqk 166 16 signal signal NN cord-104269-9r7rqqqk 166 17 has have VBZ cord-104269-9r7rqqqk 166 18 been be VBN cord-104269-9r7rqqqk 166 19 shown show VBN cord-104269-9r7rqqqk 166 20 to to TO cord-104269-9r7rqqqk 166 21 function function VB cord-104269-9r7rqqqk 166 22 as as IN cord-104269-9r7rqqqk 166 23 an an DT cord-104269-9r7rqqqk 166 24 internalization internalization NN cord-104269-9r7rqqqk 166 25 signal signal NN cord-104269-9r7rqqqk 166 26 ( ( -LRB- cord-104269-9r7rqqqk 166 27 Letourneur Letourneur NNP cord-104269-9r7rqqqk 166 28 and and CC cord-104269-9r7rqqqk 166 29 Klausner Klausner NNP cord-104269-9r7rqqqk 166 30 , , , cord-104269-9r7rqqqk 166 31 1992 1992 CD cord-104269-9r7rqqqk 166 32 ; ; : cord-104269-9r7rqqqk 166 33 White White NNP cord-104269-9r7rqqqk 166 34 , , , cord-104269-9r7rqqqk 166 35 S. S. NNP cord-104269-9r7rqqqk 166 36 , , , cord-104269-9r7rqqqk 166 37 J. J. NNP cord-104269-9r7rqqqk 166 38 F. F. NNP cord-104269-9r7rqqqk 166 39 Collawn Collawn NNP cord-104269-9r7rqqqk 166 40 , , , cord-104269-9r7rqqqk 166 41 and and CC cord-104269-9r7rqqqk 166 42 I. I. NNP cord-104269-9r7rqqqk 166 43 S. S. NNP cord-104269-9r7rqqqk 166 44 Trowbridge Trowbridge NNP cord-104269-9r7rqqqk 166 45 , , , cord-104269-9r7rqqqk 166 46 unpublished unpublished JJ cord-104269-9r7rqqqk 166 47 results result NNS cord-104269-9r7rqqqk 166 48 ) ) -RRB- cord-104269-9r7rqqqk 166 49 . . . cord-104269-9r7rqqqk 167 1 Di Di NNP cord-104269-9r7rqqqk 167 2 - - HYPH cord-104269-9r7rqqqk 167 3 leucine leucine NN cord-104269-9r7rqqqk 167 4 - - HYPH cord-104269-9r7rqqqk 167 5 based base VBN cord-104269-9r7rqqqk 167 6 sorting sorting NN cord-104269-9r7rqqqk 167 7 signals signal NNS cord-104269-9r7rqqqk 167 8 have have VBP cord-104269-9r7rqqqk 167 9 not not RB cord-104269-9r7rqqqk 167 10 been be VBN cord-104269-9r7rqqqk 167 11 extensively extensively RB cord-104269-9r7rqqqk 167 12 characterized characterize VBN cord-104269-9r7rqqqk 167 13 ; ; : cord-104269-9r7rqqqk 167 14 however however RB cord-104269-9r7rqqqk 167 15 , , , cord-104269-9r7rqqqk 167 16 isoleucine isoleucine NNP cord-104269-9r7rqqqk 167 17 can can MD cord-104269-9r7rqqqk 167 18 substitute substitute VB cord-104269-9r7rqqqk 167 19 for for IN cord-104269-9r7rqqqk 167 20 the the DT cord-104269-9r7rqqqk 167 21 second second JJ cord-104269-9r7rqqqk 167 22 leucine leucine NN cord-104269-9r7rqqqk 167 23 , , , cord-104269-9r7rqqqk 167 24 but but CC cord-104269-9r7rqqqk 167 25 not not RB cord-104269-9r7rqqqk 167 26 the the DT cord-104269-9r7rqqqk 167 27 first first JJ cord-104269-9r7rqqqk 167 28 , , , cord-104269-9r7rqqqk 167 29 in in IN cord-104269-9r7rqqqk 167 30 the the DT cord-104269-9r7rqqqk 167 31 CD3~/di cd3~/di NN cord-104269-9r7rqqqk 167 32 - - HYPH cord-104269-9r7rqqqk 167 33 leucine leucine NN cord-104269-9r7rqqqk 167 34 signal signal NN cord-104269-9r7rqqqk 167 35 without without IN cord-104269-9r7rqqqk 167 36 loss loss NN cord-104269-9r7rqqqk 167 37 of of IN cord-104269-9r7rqqqk 167 38 lysosomal lysosomal JJ cord-104269-9r7rqqqk 167 39 targeting targeting NN cord-104269-9r7rqqqk 167 40 activity activity NN cord-104269-9r7rqqqk 167 41 , , , cord-104269-9r7rqqqk 167 42 whereas whereas IN cord-104269-9r7rqqqk 167 43 alteration alteration NN cord-104269-9r7rqqqk 167 44 of of IN cord-104269-9r7rqqqk 167 45 either either DT cord-104269-9r7rqqqk 167 46 leucine leucine NN cord-104269-9r7rqqqk 167 47 to to IN cord-104269-9r7rqqqk 167 48 alanine alanine NN cord-104269-9r7rqqqk 167 49 substantially substantially RB cord-104269-9r7rqqqk 167 50 reduces reduce VBZ cord-104269-9r7rqqqk 167 51 activity activity NN cord-104269-9r7rqqqk 167 52 ( ( -LRB- cord-104269-9r7rqqqk 167 53 Letourneur Letourneur NNP cord-104269-9r7rqqqk 167 54 and and CC cord-104269-9r7rqqqk 167 55 Klausnet Klausnet NNP cord-104269-9r7rqqqk 167 56 , , , cord-104269-9r7rqqqk 167 57 1992 1992 CD cord-104269-9r7rqqqk 167 58 ) ) -RRB- cord-104269-9r7rqqqk 167 59 . . . cord-104269-9r7rqqqk 168 1 Because because IN cord-104269-9r7rqqqk 168 2 of of IN cord-104269-9r7rqqqk 168 3 the the DT cord-104269-9r7rqqqk 168 4 stricter strict JJR cord-104269-9r7rqqqk 168 5 requirement requirement NN cord-104269-9r7rqqqk 168 6 for for IN cord-104269-9r7rqqqk 168 7 leucine leucine NN cord-104269-9r7rqqqk 168 8 in in IN cord-104269-9r7rqqqk 168 9 the the DT cord-104269-9r7rqqqk 168 10 first first JJ cord-104269-9r7rqqqk 168 11 position position NN cord-104269-9r7rqqqk 168 12 of of IN cord-104269-9r7rqqqk 168 13 the the DT cord-104269-9r7rqqqk 168 14 CD3~ CD3~ NNP cord-104269-9r7rqqqk 168 15 , , , cord-104269-9r7rqqqk 168 16 chain chain NN cord-104269-9r7rqqqk 168 17 signal signal NN cord-104269-9r7rqqqk 168 18 , , , cord-104269-9r7rqqqk 168 19 we -PRON- PRP cord-104269-9r7rqqqk 168 20 initially initially RB cord-104269-9r7rqqqk 168 21 thought think VBD cord-104269-9r7rqqqk 168 22 that that IN cord-104269-9r7rqqqk 168 23 the the DT cord-104269-9r7rqqqk 168 24 two two CD cord-104269-9r7rqqqk 168 25 internalization internalization NN cord-104269-9r7rqqqk 168 26 signals signal NNS cord-104269-9r7rqqqk 168 27 identified identify VBN cord-104269-9r7rqqqk 168 28 by by IN cord-104269-9r7rqqqk 168 29 deletional deletional JJ cord-104269-9r7rqqqk 168 30 analysis analysis NN cord-104269-9r7rqqqk 168 31 may may MD cord-104269-9r7rqqqk 168 32 involve involve VB cord-104269-9r7rqqqk 168 33 I.,¢uTIle I.,¢uTIle NNP cord-104269-9r7rqqqk 168 34 s s NN cord-104269-9r7rqqqk 168 35 and and CC cord-104269-9r7rqqqk 168 36 Leu~4pro Leu~4pro NNP cord-104269-9r7rqqqk 168 37 is be VBZ cord-104269-9r7rqqqk 168 38 , , , cord-104269-9r7rqqqk 168 39 respectively respectively RB cord-104269-9r7rqqqk 168 40 . . . cord-104269-9r7rqqqk 169 1 To to TO cord-104269-9r7rqqqk 169 2 investigate investigate VB cord-104269-9r7rqqqk 169 3 this this DT cord-104269-9r7rqqqk 169 4 possibility possibility NN cord-104269-9r7rqqqk 169 5 , , , cord-104269-9r7rqqqk 169 6 the the DT cord-104269-9r7rqqqk 169 7 two two CD cord-104269-9r7rqqqk 169 8 residues residue NNS cord-104269-9r7rqqqk 169 9 of of IN cord-104269-9r7rqqqk 169 10 either either DT cord-104269-9r7rqqqk 169 11 one one CD cord-104269-9r7rqqqk 169 12 or or CC cord-104269-9r7rqqqk 169 13 both both DT cord-104269-9r7rqqqk 169 14 of of IN cord-104269-9r7rqqqk 169 15 these these DT cord-104269-9r7rqqqk 169 16 di di NN cord-104269-9r7rqqqk 169 17 - - HYPH cord-104269-9r7rqqqk 169 18 peptides peptide NNS cord-104269-9r7rqqqk 169 19 were be VBD cord-104269-9r7rqqqk 169 20 altered alter VBN cord-104269-9r7rqqqk 169 21 to to IN cord-104269-9r7rqqqk 169 22 alanine alanine NN cord-104269-9r7rqqqk 169 23 . . . cord-104269-9r7rqqqk 170 1 Strikingly strikingly RB cord-104269-9r7rqqqk 170 2 , , , cord-104269-9r7rqqqk 170 3 independently independently RB cord-104269-9r7rqqqk 170 4 altering alter VBG cord-104269-9r7rqqqk 170 5 each each DT cord-104269-9r7rqqqk 170 6 di di NN cord-104269-9r7rqqqk 170 7 - - HYPH cord-104269-9r7rqqqk 170 8 peptide peptide JJ cord-104269-9r7rqqqk 170 9 to to IN cord-104269-9r7rqqqk 170 10 two two CD cord-104269-9r7rqqqk 170 11 alanine alanine NN cord-104269-9r7rqqqk 170 12 residues residue NNS cord-104269-9r7rqqqk 170 13 reduced reduce VBD cord-104269-9r7rqqqk 170 14 the the DT cord-104269-9r7rqqqk 170 15 apparent apparent JJ cord-104269-9r7rqqqk 170 16 internalization internalization NN cord-104269-9r7rqqqk 170 17 efficiency efficiency NN cord-104269-9r7rqqqk 170 18 of of IN cord-104269-9r7rqqqk 170 19 the the DT cord-104269-9r7rqqqk 170 20 Iicr Iicr NNP cord-104269-9r7rqqqk 170 21 chimera chimera NN cord-104269-9r7rqqqk 170 22 by by IN cord-104269-9r7rqqqk 170 23 ~,50 ~,50 CD cord-104269-9r7rqqqk 170 24 % % NN cord-104269-9r7rqqqk 170 25 , , , cord-104269-9r7rqqqk 170 26 whereas whereas IN cord-104269-9r7rqqqk 170 27 alteration alteration NN cord-104269-9r7rqqqk 170 28 of of IN cord-104269-9r7rqqqk 170 29 both both DT cord-104269-9r7rqqqk 170 30 dipeptides dipeptide NNS cord-104269-9r7rqqqk 170 31 completely completely RB cord-104269-9r7rqqqk 170 32 abrogated abrogate VBD cord-104269-9r7rqqqk 170 33 high high JJ cord-104269-9r7rqqqk 170 34 efficiency efficiency NN cord-104269-9r7rqqqk 170 35 endocytosis endocytosis NN cord-104269-9r7rqqqk 170 36 ( ( -LRB- cord-104269-9r7rqqqk 170 37 Table table NN cord-104269-9r7rqqqk 170 38 ID ID NNP cord-104269-9r7rqqqk 170 39 . . . cord-104269-9r7rqqqk 171 1 These these DT cord-104269-9r7rqqqk 171 2 results result NNS cord-104269-9r7rqqqk 171 3 supported support VBD cord-104269-9r7rqqqk 171 4 the the DT cord-104269-9r7rqqqk 171 5 idea idea NN cord-104269-9r7rqqqk 171 6 that that IN cord-104269-9r7rqqqk 171 7 the the DT cord-104269-9r7rqqqk 171 8 Ii Ii NNP cord-104269-9r7rqqqk 171 9 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 171 10 tail tail NN cord-104269-9r7rqqqk 171 11 contains contain VBZ cord-104269-9r7rqqqk 171 12 two two CD cord-104269-9r7rqqqk 171 13 non non JJ cord-104269-9r7rqqqk 171 14 - - JJ cord-104269-9r7rqqqk 171 15 tyrosine tyrosine NN cord-104269-9r7rqqqk 171 16 - - HYPH cord-104269-9r7rqqqk 171 17 based base VBN cord-104269-9r7rqqqk 171 18 signals signal NNS cord-104269-9r7rqqqk 171 19 with with IN cord-104269-9r7rqqqk 171 20 similar similar JJ cord-104269-9r7rqqqk 171 21 internalization internalization NN cord-104269-9r7rqqqk 171 22 activity activity NN cord-104269-9r7rqqqk 171 23 that that WDT cord-104269-9r7rqqqk 171 24 are be VBP cord-104269-9r7rqqqk 171 25 related relate VBN cord-104269-9r7rqqqk 171 26 to to IN cord-104269-9r7rqqqk 171 27 the the DT cord-104269-9r7rqqqk 171 28 CD3"y CD3"y NNP cord-104269-9r7rqqqk 171 29 chain chain NN cord-104269-9r7rqqqk 171 30 di di NN cord-104269-9r7rqqqk 171 31 - - HYPH cord-104269-9r7rqqqk 171 32 leucine leucine JJ cord-104269-9r7rqqqk 171 33 signal signal NN cord-104269-9r7rqqqk 171 34 . . . cord-104269-9r7rqqqk 172 1 To to TO cord-104269-9r7rqqqk 172 2 further further RB cord-104269-9r7rqqqk 172 3 characterize characterize VB cord-104269-9r7rqqqk 172 4 the the DT cord-104269-9r7rqqqk 172 5 two two CD cord-104269-9r7rqqqk 172 6 sorting sorting NN cord-104269-9r7rqqqk 172 7 signals signal NNS cord-104269-9r7rqqqk 172 8 , , , cord-104269-9r7rqqqk 172 9 each each DT cord-104269-9r7rqqqk 172 10 of of IN cord-104269-9r7rqqqk 172 11 the the DT cord-104269-9r7rqqqk 172 12 two two CD cord-104269-9r7rqqqk 172 13 residues residue NNS cord-104269-9r7rqqqk 172 14 that that WDT cord-104269-9r7rqqqk 172 15 might may MD cord-104269-9r7rqqqk 172 16 be be VB cord-104269-9r7rqqqk 172 17 important important JJ cord-104269-9r7rqqqk 172 18 for for IN cord-104269-9r7rqqqk 172 19 their -PRON- PRP$ cord-104269-9r7rqqqk 172 20 activity activity NN cord-104269-9r7rqqqk 172 21 were be VBD cord-104269-9r7rqqqk 172 22 independently independently RB cord-104269-9r7rqqqk 172 23 changed change VBN cord-104269-9r7rqqqk 172 24 to to IN cord-104269-9r7rqqqk 172 25 alanine alanine NN cord-104269-9r7rqqqk 172 26 in in IN cord-104269-9r7rqqqk 172 27 mutant mutant JJ cord-104269-9r7rqqqk 172 28 receptors receptor NNS cord-104269-9r7rqqqk 172 29 in in IN cord-104269-9r7rqqqk 172 30 which which WDT cord-104269-9r7rqqqk 172 31 the the DT cord-104269-9r7rqqqk 172 32 second second JJ cord-104269-9r7rqqqk 172 33 signal signal NN cord-104269-9r7rqqqk 172 34 had have VBD cord-104269-9r7rqqqk 172 35 been be VBN cord-104269-9r7rqqqk 172 36 inactivated inactivate VBN cord-104269-9r7rqqqk 172 37 by by IN cord-104269-9r7rqqqk 172 38 alanine alanine JJ cord-104269-9r7rqqqk 172 39 substitutions substitution NNS cord-104269-9r7rqqqk 172 40 . . . cord-104269-9r7rqqqk 173 1 The the DT cord-104269-9r7rqqqk 173 2 results result NNS cord-104269-9r7rqqqk 173 3 of of IN cord-104269-9r7rqqqk 173 4 this this DT cord-104269-9r7rqqqk 173 5 analysis analysis NN cord-104269-9r7rqqqk 173 6 clearly clearly RB cord-104269-9r7rqqqk 173 7 showed show VBD cord-104269-9r7rqqqk 173 8 that that IN cord-104269-9r7rqqqk 173 9 both both CC cord-104269-9r7rqqqk 173 10 Leu Leu NNP cord-104269-9r7rqqqk 173 11 7 7 CD cord-104269-9r7rqqqk 173 12 and and CC cord-104269-9r7rqqqk 173 13 He -PRON- PRP cord-104269-9r7rqqqk 173 14 S S VBZ cord-104269-9r7rqqqk 173 15 were be VBD cord-104269-9r7rqqqk 173 16 required require VBN cord-104269-9r7rqqqk 173 17 for for IN cord-104269-9r7rqqqk 173 18 the the DT cord-104269-9r7rqqqk 173 19 signal signal NN cord-104269-9r7rqqqk 173 20 most most RBS cord-104269-9r7rqqqk 173 21 distal distal JJ cord-104269-9r7rqqqk 173 22 from from IN cord-104269-9r7rqqqk 173 23 the the DT cord-104269-9r7rqqqk 173 24 transmembrane transmembrane NN cord-104269-9r7rqqqk 173 25 region region NN cord-104269-9r7rqqqk 173 26 ( ( -LRB- cord-104269-9r7rqqqk 173 27 Table table NN cord-104269-9r7rqqqk 173 28 HI HI NNP cord-104269-9r7rqqqk 173 29 ) ) -RRB- cord-104269-9r7rqqqk 173 30 . . . cord-104269-9r7rqqqk 174 1 However however RB cord-104269-9r7rqqqk 174 2 , , , cord-104269-9r7rqqqk 174 3 whereas whereas IN cord-104269-9r7rqqqk 174 4 alteration alteration NN cord-104269-9r7rqqqk 174 5 of of IN cord-104269-9r7rqqqk 174 6 Pro Pro NNP cord-104269-9r7rqqqk 174 7 ~s ~s NNP cord-104269-9r7rqqqk 174 8 to to IN cord-104269-9r7rqqqk 174 9 alanine alanine NN cord-104269-9r7rqqqk 174 10 abolished abolish VBD cord-104269-9r7rqqqk 174 11 the the DT cord-104269-9r7rqqqk 174 12 internalization internalization NN cord-104269-9r7rqqqk 174 13 activity activity NN cord-104269-9r7rqqqk 174 14 of of IN cord-104269-9r7rqqqk 174 15 the the DT cord-104269-9r7rqqqk 174 16 membrane membrane NN cord-104269-9r7rqqqk 174 17 - - HYPH cord-104269-9r7rqqqk 174 18 proximal proximal JJ cord-104269-9r7rqqqk 174 19 signal signal NN cord-104269-9r7rqqqk 174 20 , , , cord-104269-9r7rqqqk 174 21 alteration alteration NN cord-104269-9r7rqqqk 174 22 of of IN cord-104269-9r7rqqqk 174 23 Leu Leu NNP cord-104269-9r7rqqqk 174 24 14 14 CD cord-104269-9r7rqqqk 174 25 to to IN cord-104269-9r7rqqqk 174 26 alanine alanine NNP cord-104269-9r7rqqqk 174 27 increased increase VBD cord-104269-9r7rqqqk 174 28 its -PRON- PRP$ cord-104269-9r7rqqqk 174 29 efficiency efficiency NN cord-104269-9r7rqqqk 174 30 . . . cord-104269-9r7rqqqk 175 1 This this DT cord-104269-9r7rqqqk 175 2 result result NN cord-104269-9r7rqqqk 175 3 implied imply VBD cord-104269-9r7rqqqk 175 4 that that IN cord-104269-9r7rqqqk 175 5 the the DT cord-104269-9r7rqqqk 175 6 membrane membrane NN cord-104269-9r7rqqqk 175 7 - - HYPH cord-104269-9r7rqqqk 175 8 proximal proximal JJ cord-104269-9r7rqqqk 175 9 signal signal NN cord-104269-9r7rqqqk 175 10 was be VBD cord-104269-9r7rqqqk 175 11 novel novel JJ cord-104269-9r7rqqqk 175 12 and and CC cord-104269-9r7rqqqk 175 13 led lead VBD cord-104269-9r7rqqqk 175 14 us -PRON- PRP cord-104269-9r7rqqqk 175 15 to to TO cord-104269-9r7rqqqk 175 16 examine examine VB cord-104269-9r7rqqqk 175 17 whether whether IN cord-104269-9r7rqqqk 175 18 the the DT cord-104269-9r7rqqqk 175 19 two two CD cord-104269-9r7rqqqk 175 20 hydrophobic hydrophobic JJ cord-104269-9r7rqqqk 175 21 residues residue NNS cord-104269-9r7rqqqk 175 22 , , , cord-104269-9r7rqqqk 175 23 Met Met NNP cord-104269-9r7rqqqk 175 24 ~6 ~6 NFP cord-104269-9r7rqqqk 175 25 and and CC cord-104269-9r7rqqqk 175 26 Leu Leu NNP cord-104269-9r7rqqqk 175 27 ~7 ~7 NNP cord-104269-9r7rqqqk 175 28 , , , cord-104269-9r7rqqqk 175 29 on on IN cord-104269-9r7rqqqk 175 30 the the DT cord-104269-9r7rqqqk 175 31 carboxy carboxy JJ cord-104269-9r7rqqqk 175 32 - - HYPH cord-104269-9r7rqqqk 175 33 terminal terminal NN cord-104269-9r7rqqqk 175 34 side side NN cord-104269-9r7rqqqk 175 35 of of IN cord-104269-9r7rqqqk 175 36 Pro Pro NNP cord-104269-9r7rqqqk 175 37 ~5 ~5 NNP cord-104269-9r7rqqqk 175 38 were be VBD cord-104269-9r7rqqqk 175 39 required require VBN cord-104269-9r7rqqqk 175 40 . . . cord-104269-9r7rqqqk 176 1 As as IN cord-104269-9r7rqqqk 176 2 shown show VBN cord-104269-9r7rqqqk 176 3 in in IN cord-104269-9r7rqqqk 176 4 Table Table NNP cord-104269-9r7rqqqk 176 5 HI HI NNP cord-104269-9r7rqqqk 176 6 , , , cord-104269-9r7rqqqk 176 7 alteration alteration NN cord-104269-9r7rqqqk 176 8 of of IN cord-104269-9r7rqqqk 176 9 either either DT cord-104269-9r7rqqqk 176 10 Met Met NNP cord-104269-9r7rqqqk 176 11 ~6 ~6 CD cord-104269-9r7rqqqk 176 12 or or CC cord-104269-9r7rqqqk 176 13 Leu Leu NNP cord-104269-9r7rqqqk 176 14 17 17 CD cord-104269-9r7rqqqk 176 15 to to IN cord-104269-9r7rqqqk 176 16 alanine alanine NNP cord-104269-9r7rqqqk 176 17 completely completely RB cord-104269-9r7rqqqk 176 18 abrogated abrogate VBD cord-104269-9r7rqqqk 176 19 the the DT cord-104269-9r7rqqqk 176 20 activity activity NN cord-104269-9r7rqqqk 176 21 of of IN cord-104269-9r7rqqqk 176 22 the the DT cord-104269-9r7rqqqk 176 23 membrane membrane NN cord-104269-9r7rqqqk 176 24 - - HYPH cord-104269-9r7rqqqk 176 25 proximal proximal JJ cord-104269-9r7rqqqk 176 26 sorting sorting NN cord-104269-9r7rqqqk 176 27 signal signal NN cord-104269-9r7rqqqk 176 28 in in IN cord-104269-9r7rqqqk 176 29 the the DT cord-104269-9r7rqqqk 176 30 Ii Ii NNP cord-104269-9r7rqqqk 176 31 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 176 32 tall tall JJ cord-104269-9r7rqqqk 176 33 . . . cord-104269-9r7rqqqk 177 1 We -PRON- PRP cord-104269-9r7rqqqk 177 2 conclude conclude VBP cord-104269-9r7rqqqk 177 3 , , , cord-104269-9r7rqqqk 177 4 therefore therefore RB cord-104269-9r7rqqqk 177 5 , , , cord-104269-9r7rqqqk 177 6 that that IN cord-104269-9r7rqqqk 177 7 one one CD cord-104269-9r7rqqqk 177 8 internalization internalization NN cord-104269-9r7rqqqk 177 9 signal signal NN cord-104269-9r7rqqqk 177 10 in in IN cord-104269-9r7rqqqk 177 11 the the DT cord-104269-9r7rqqqk 177 12 Ii Ii NNP cord-104269-9r7rqqqk 177 13 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 177 14 taft taft NNP cord-104269-9r7rqqqk 177 15 involves involve VBZ cord-104269-9r7rqqqk 177 16 Leu Leu NNP cord-104269-9r7rqqqk 177 17 7 7 CD cord-104269-9r7rqqqk 177 18 and and CC cord-104269-9r7rqqqk 177 19 Ile Ile NNP cord-104269-9r7rqqqk 177 20 s s NNP cord-104269-9r7rqqqk 177 21 , , , cord-104269-9r7rqqqk 177 22 whereas whereas IN cord-104269-9r7rqqqk 177 23 Pro Pro NNP cord-104269-9r7rqqqk 177 24 15 15 CD cord-104269-9r7rqqqk 177 25 , , , cord-104269-9r7rqqqk 177 26 Met Met NNP cord-104269-9r7rqqqk 177 27 16 16 CD cord-104269-9r7rqqqk 177 28 , , , cord-104269-9r7rqqqk 177 29 or or CC cord-104269-9r7rqqqk 177 30 Leu Leu NNP cord-104269-9r7rqqqk 177 31 ~7 ~7 NNP cord-104269-9r7rqqqk 177 32 are be VBP cord-104269-9r7rqqqk 177 33 all all RB cord-104269-9r7rqqqk 177 34 important important JJ cord-104269-9r7rqqqk 177 35 for for IN cord-104269-9r7rqqqk 177 36 the the DT cord-104269-9r7rqqqk 177 37 second second JJ cord-104269-9r7rqqqk 177 38 signal signal NN cord-104269-9r7rqqqk 177 39 . . . cord-104269-9r7rqqqk 178 1 Consistent consistent JJ cord-104269-9r7rqqqk 178 2 with with IN cord-104269-9r7rqqqk 178 3 the the DT cord-104269-9r7rqqqk 178 4 distal distal JJ cord-104269-9r7rqqqk 178 5 signal signal NN cord-104269-9r7rqqqk 178 6 being be VBG cord-104269-9r7rqqqk 178 7 closely closely RB cord-104269-9r7rqqqk 178 8 related relate VBN cord-104269-9r7rqqqk 178 9 to to IN cord-104269-9r7rqqqk 178 10 the the DT cord-104269-9r7rqqqk 178 11 CD33 CD33 NNP cord-104269-9r7rqqqk 178 12 , , , cord-104269-9r7rqqqk 178 13 chain chain NN cord-104269-9r7rqqqk 178 14 di di NN cord-104269-9r7rqqqk 178 15 - - HYPH cord-104269-9r7rqqqk 178 16 leucine leucine JJ cord-104269-9r7rqqqk 178 17 signal signal NN cord-104269-9r7rqqqk 178 18 , , , cord-104269-9r7rqqqk 178 19 Ile Ile NNP cord-104269-9r7rqqqk 178 20 s s PRP cord-104269-9r7rqqqk 178 21 could could MD cord-104269-9r7rqqqk 178 22 be be VB cord-104269-9r7rqqqk 178 23 modified modify VBN cord-104269-9r7rqqqk 178 24 to to IN cord-104269-9r7rqqqk 178 25 leucine leucine NN cord-104269-9r7rqqqk 178 26 without without IN cord-104269-9r7rqqqk 178 27 loss loss NN cord-104269-9r7rqqqk 178 28 of of IN cord-104269-9r7rqqqk 178 29 activity activity NN cord-104269-9r7rqqqk 178 30 . . . cord-104269-9r7rqqqk 179 1 Interestingly interestingly RB cord-104269-9r7rqqqk 179 2 , , , cord-104269-9r7rqqqk 179 3 the the DT cord-104269-9r7rqqqk 179 4 nonconservative nonconservative JJ cord-104269-9r7rqqqk 179 5 mutation mutation NN cord-104269-9r7rqqqk 179 6 of of IN cord-104269-9r7rqqqk 179 7 Pro Pro NNP cord-104269-9r7rqqqk 179 8 ~5 ~5 NNP cord-104269-9r7rqqqk 179 9 to to IN cord-104269-9r7rqqqk 179 10 leucine leucine NN cord-104269-9r7rqqqk 179 11 did do VBD cord-104269-9r7rqqqk 179 12 not not RB cord-104269-9r7rqqqk 179 13 reduce reduce VB cord-104269-9r7rqqqk 179 14 the the DT cord-104269-9r7rqqqk 179 15 internaliTation internalitation NN cord-104269-9r7rqqqk 179 16 activity activity NN cord-104269-9r7rqqqk 179 17 of of IN cord-104269-9r7rqqqk 179 18 the the DT cord-104269-9r7rqqqk 179 19 membrane membrane NN cord-104269-9r7rqqqk 179 20 - - HYPH cord-104269-9r7rqqqk 179 21 proximal proximal JJ cord-104269-9r7rqqqk 179 22 signal signal NN cord-104269-9r7rqqqk 179 23 ( ( -LRB- cord-104269-9r7rqqqk 179 24 Table table NN cord-104269-9r7rqqqk 179 25 HI HI NNP cord-104269-9r7rqqqk 179 26 ) ) -RRB- cord-104269-9r7rqqqk 179 27 . . . cord-104269-9r7rqqqk 180 1 Ii Ii NNP cord-104269-9r7rqqqk 180 2 is be VBZ cord-104269-9r7rqqqk 180 3 thought think VBN cord-104269-9r7rqqqk 180 4 to to TO cord-104269-9r7rqqqk 180 5 be be VB cord-104269-9r7rqqqk 180 6 transported transport VBN cord-104269-9r7rqqqk 180 7 as as IN cord-104269-9r7rqqqk 180 8 an an DT cord-104269-9r7rqqqk 180 9 ot/~Ii ot/~ii CD cord-104269-9r7rqqqk 180 10 complex complex NN cord-104269-9r7rqqqk 180 11 via via IN cord-104269-9r7rqqqk 180 12 the the DT cord-104269-9r7rqqqk 180 13 trans trans NNP cord-104269-9r7rqqqk 180 14 - - NNP cord-104269-9r7rqqqk 180 15 Golgi Golgi NNP cord-104269-9r7rqqqk 180 16 directly directly RB cord-104269-9r7rqqqk 180 17 to to IN cord-104269-9r7rqqqk 180 18 an an DT cord-104269-9r7rqqqk 180 19 acidic acidic JJ cord-104269-9r7rqqqk 180 20 endocytic endocytic JJ cord-104269-9r7rqqqk 180 21 compathaent compathaent NN cord-104269-9r7rqqqk 180 22 where where WRB cord-104269-9r7rqqqk 180 23 it -PRON- PRP cord-104269-9r7rqqqk 180 24 dissociates dissociate VBZ cord-104269-9r7rqqqk 180 25 and and CC cord-104269-9r7rqqqk 180 26 is be VBZ cord-104269-9r7rqqqk 180 27 rapidly rapidly RB cord-104269-9r7rqqqk 180 28 degraded degrade VBN cord-104269-9r7rqqqk 180 29 ( ( -LRB- cord-104269-9r7rqqqk 180 30 Cresswell Cresswell NNP cord-104269-9r7rqqqk 180 31 , , , cord-104269-9r7rqqqk 180 32 1985 1985 CD cord-104269-9r7rqqqk 180 33 ; ; : cord-104269-9r7rqqqk 180 34 Lamb Lamb NNP cord-104269-9r7rqqqk 180 35 et et NNP cord-104269-9r7rqqqk 180 36 al al NNP cord-104269-9r7rqqqk 180 37 . . NNP cord-104269-9r7rqqqk 180 38 , , , cord-104269-9r7rqqqk 180 39 1991 1991 CD cord-104269-9r7rqqqk 180 40 ; ; : cord-104269-9r7rqqqk 181 1 Pieters Pieters NNP cord-104269-9r7rqqqk 181 2 et et NNP cord-104269-9r7rqqqk 181 3 al al NNP cord-104269-9r7rqqqk 181 4 . . NNP cord-104269-9r7rqqqk 181 5 , , , cord-104269-9r7rqqqk 181 6 1991 1991 CD cord-104269-9r7rqqqk 181 7 ) ) -RRB- cord-104269-9r7rqqqk 181 8 . . . cord-104269-9r7rqqqk 182 1 To to TO cord-104269-9r7rqqqk 182 2 determine determine VB cord-104269-9r7rqqqk 182 3 whether whether IN cord-104269-9r7rqqqk 182 4 the the DT cord-104269-9r7rqqqk 182 5 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 182 6 tail tail NN cord-104269-9r7rqqqk 182 7 of of IN cord-104269-9r7rqqqk 182 8 Ii Ii NNP cord-104269-9r7rqqqk 182 9 was be VBD cord-104269-9r7rqqqk 182 10 sufficient sufficient JJ cord-104269-9r7rqqqk 182 11 to to TO cord-104269-9r7rqqqk 182 12 target target VB cord-104269-9r7rqqqk 182 13 TR TR NNP cord-104269-9r7rqqqk 182 14 to to IN cord-104269-9r7rqqqk 182 15 this this DT cord-104269-9r7rqqqk 182 16 endocytic endocytic JJ cord-104269-9r7rqqqk 182 17 compartment compartment NN cord-104269-9r7rqqqk 182 18 and and CC cord-104269-9r7rqqqk 182 19 whether whether IN cord-104269-9r7rqqqk 182 20 the the DT cord-104269-9r7rqqqk 182 21 non non JJ cord-104269-9r7rqqqk 182 22 - - JJ cord-104269-9r7rqqqk 182 23 tyrosine tyrosine NN cord-104269-9r7rqqqk 182 24 - - HYPH cord-104269-9r7rqqqk 182 25 based base VBN cord-104269-9r7rqqqk 182 26 sorting sorting NN cord-104269-9r7rqqqk 182 27 signals signal NNS cord-104269-9r7rqqqk 182 28 were be VBD cord-104269-9r7rqqqk 182 29 involved involve VBN cord-104269-9r7rqqqk 182 30 , , , cord-104269-9r7rqqqk 182 31 metabolic metabolic JJ cord-104269-9r7rqqqk 182 32 pulse- pulse- NN cord-104269-9r7rqqqk 183 1 * * NFP cord-104269-9r7rqqqk 183 2 The the DT cord-104269-9r7rqqqk 183 3 LT--*'A LT--*'A NNP cord-104269-9r7rqqqk 183 4 , , , cord-104269-9r7rqqqk 183 5 P"*A P"*A NNP cord-104269-9r7rqqqk 183 6 , , , cord-104269-9r7rqqqk 183 7 and and CC cord-104269-9r7rqqqk 183 8 P p NN cord-104269-9r7rqqqk 183 9 ~ ~ NFP cord-104269-9r7rqqqk 183 10 L l NN cord-104269-9r7rqqqk 183 11 mutations mutation NNS cord-104269-9r7rqqqk 183 12 were be VBD cord-104269-9r7rqqqk 183 13 introduced introduce VBN cord-104269-9r7rqqqk 183 14 into into IN cord-104269-9r7rqqqk 183 15 the the DT cord-104269-9r7rqqqk 183 16 mutant mutant JJ cord-104269-9r7rqqqk 183 17 Iicr Iicr NNP cord-104269-9r7rqqqk 183 18 LP-*AA LP-*AA NNP cord-104269-9r7rqqqk 183 19 construct construct NN cord-104269-9r7rqqqk 183 20 ( ( -LRB- cord-104269-9r7rqqqk 183 21 internalization internalization NN cord-104269-9r7rqqqk 183 22 efficiency efficiency NN cord-104269-9r7rqqqk 183 23 of of IN cord-104269-9r7rqqqk 183 24 57 57 CD cord-104269-9r7rqqqk 183 25 % % NN cord-104269-9r7rqqqk 183 26 relative relative JJ cord-104269-9r7rqqqk 183 27 to to IN cord-104269-9r7rqqqk 183 28 the the DT cord-104269-9r7rqqqk 183 29 Iicr Iicr NNP cord-104269-9r7rqqqk 183 30 chimera chimera NN cord-104269-9r7rqqqk 183 31 ; ; : cord-104269-9r7rqqqk 183 32 see see VB cord-104269-9r7rqqqk 183 33 Table Table NNP cord-104269-9r7rqqqk 183 34 II II NNP cord-104269-9r7rqqqk 183 35 ) ) -RRB- cord-104269-9r7rqqqk 183 36 , , , cord-104269-9r7rqqqk 183 37 and and CC cord-104269-9r7rqqqk 183 38 the the DT cord-104269-9r7rqqqk 183 39 L~'-~A L~'-~A NNS cord-104269-9r7rqqqk 183 40 , , , cord-104269-9r7rqqqk 183 41 PlS PlS NNP cord-104269-9r7rqqqk 183 42 ~ ~ NFP cord-104269-9r7rqqqk 183 43 A A NNP cord-104269-9r7rqqqk 183 44 , , , cord-104269-9r7rqqqk 183 45 P p NN cord-104269-9r7rqqqk 183 46 ~ ~ CD cord-104269-9r7rqqqk 183 47 s-"L s-"l NN cord-104269-9r7rqqqk 183 48 , , , cord-104269-9r7rqqqk 183 49 M16~A M16~A NNP cord-104269-9r7rqqqk 183 50 , , , cord-104269-9r7rqqqk 183 51 and and CC cord-104269-9r7rqqqk 183 52 LIT-*A LIT-*A NNS cord-104269-9r7rqqqk 183 53 mutations mutation NNS cord-104269-9r7rqqqk 183 54 were be VBD cord-104269-9r7rqqqk 183 55 introduced introduce VBN cord-104269-9r7rqqqk 183 56 into into IN cord-104269-9r7rqqqk 183 57 the the DT cord-104269-9r7rqqqk 183 58 mutant mutant JJ cord-104269-9r7rqqqk 183 59 Iicr Iicr NNP cord-104269-9r7rqqqk 183 60 LI LI NNP cord-104269-9r7rqqqk 183 61 ~ ~ NFP cord-104269-9r7rqqqk 183 62 AA AA NNP cord-104269-9r7rqqqk 183 63 construct construct NN cord-104269-9r7rqqqk 183 64 ( ( -LRB- cord-104269-9r7rqqqk 183 65 internalization internalization NN cord-104269-9r7rqqqk 183 66 efficiency efficiency NN cord-104269-9r7rqqqk 183 67 of of IN cord-104269-9r7rqqqk 183 68 50 50 CD cord-104269-9r7rqqqk 183 69 % % NN cord-104269-9r7rqqqk 183 70 relative relative JJ cord-104269-9r7rqqqk 183 71 to to IN cord-104269-9r7rqqqk 183 72 the the DT cord-104269-9r7rqqqk 183 73 Iicr Iicr NNP cord-104269-9r7rqqqk 183 74 chimera chimera NN cord-104269-9r7rqqqk 183 75 ; ; : cord-104269-9r7rqqqk 183 76 see see VB cord-104269-9r7rqqqk 183 77 Table Table NNP cord-104269-9r7rqqqk 183 78 II II NNP cord-104269-9r7rqqqk 183 79 ) ) -RRB- cord-104269-9r7rqqqk 183 80 . . . cord-104269-9r7rqqqk 184 1 Internalization internalization NN cord-104269-9r7rqqqk 184 2 efficiencies efficiency NNS cord-104269-9r7rqqqk 184 3 of of IN cord-104269-9r7rqqqk 184 4 mutant mutant JJ cord-104269-9r7rqqqk 184 5 Iicr Iicr NNP cord-104269-9r7rqqqk 184 6 chimeras chimera NNS cord-104269-9r7rqqqk 184 7 are be VBP cord-104269-9r7rqqqk 184 8 expressed express VBN cord-104269-9r7rqqqk 184 9 relative relative JJ cord-104269-9r7rqqqk 184 10 to to IN cord-104269-9r7rqqqk 184 11 the the DT cord-104269-9r7rqqqk 184 12 Iic Iic NNP cord-104269-9r7rqqqk 184 13 - - HYPH cord-104269-9r7rqqqk 184 14 r r NNP cord-104269-9r7rqqqk 184 15 chimeras chimera NNS cord-104269-9r7rqqqk 184 16 as as IN cord-104269-9r7rqqqk 184 17 described describe VBN cord-104269-9r7rqqqk 184 18 in in IN cord-104269-9r7rqqqk 184 19 Table Table NNP cord-104269-9r7rqqqk 184 20 II II NNP cord-104269-9r7rqqqk 184 21 Figure Figure NNP cord-104269-9r7rqqqk 184 22 2 2 CD cord-104269-9r7rqqqk 184 23 . . . cord-104269-9r7rqqqk 185 1 Rapid rapid JJ cord-104269-9r7rqqqk 185 2 degradation degradation NN cord-104269-9r7rqqqk 185 3 of of IN cord-104269-9r7rqqqk 185 4 Ii Ii NNP cord-104269-9r7rqqqk 185 5 - - HYPH cord-104269-9r7rqqqk 185 6 TR TR NNP cord-104269-9r7rqqqk 185 7 chimeras chimera NNS cord-104269-9r7rqqqk 185 8 in in IN cord-104269-9r7rqqqk 185 9 a a DT cord-104269-9r7rqqqk 185 10 post post JJ cord-104269-9r7rqqqk 185 11 - - JJ cord-104269-9r7rqqqk 185 12 Golgi Golgi NNP cord-104269-9r7rqqqk 185 13 endocytic endocytic JJ cord-104269-9r7rqqqk 185 14 compartment compartment NN cord-104269-9r7rqqqk 185 15 . . . cord-104269-9r7rqqqk 186 1 Equivalent equivalent JJ cord-104269-9r7rqqqk 186 2 cell cell NN cord-104269-9r7rqqqk 186 3 numbers number NNS cord-104269-9r7rqqqk 186 4 of of IN cord-104269-9r7rqqqk 186 5 CEF CEF NNP cord-104269-9r7rqqqk 186 6 expressing express VBG cord-104269-9r7rqqqk 186 7 either either CC cord-104269-9r7rqqqk 186 8 wild wild JJ cord-104269-9r7rqqqk 186 9 - - HYPH cord-104269-9r7rqqqk 186 10 type type NN cord-104269-9r7rqqqk 186 11 TR tr NN cord-104269-9r7rqqqk 186 12 or or CC cord-104269-9r7rqqqk 186 13 the the DT cord-104269-9r7rqqqk 186 14 Ii Ii NNP cord-104269-9r7rqqqk 186 15 - - HYPH cord-104269-9r7rqqqk 186 16 TR TR NNP cord-104269-9r7rqqqk 186 17 chimeras chimera NNS cord-104269-9r7rqqqk 186 18 , , , cord-104269-9r7rqqqk 186 19 Iicr Iicr NNP cord-104269-9r7rqqqk 186 20 , , , cord-104269-9r7rqqqk 186 21 Iier+xM iier+xm JJ cord-104269-9r7rqqqk 186 22 , , , cord-104269-9r7rqqqk 186 23 or or CC cord-104269-9r7rqqqk 186 24 Iicr Iicr NNP cord-104269-9r7rqqqk 186 25 LI LI NNP cord-104269-9r7rqqqk 186 26 , , , cord-104269-9r7rqqqk 187 1 LP--*AA LP--*AA NNP cord-104269-9r7rqqqk 188 1 , , , cord-104269-9r7rqqqk 188 2 AA AA NNP cord-104269-9r7rqqqk 188 3 were be VBD cord-104269-9r7rqqqk 188 4 pulselabeled pulselabele VBN cord-104269-9r7rqqqk 188 5 for for IN cord-104269-9r7rqqqk 188 6 30 30 CD cord-104269-9r7rqqqk 188 7 rain rain NN cord-104269-9r7rqqqk 188 8 with with IN cord-104269-9r7rqqqk 188 9 trans-35S trans-35S NNP cord-104269-9r7rqqqk 188 10 - - HYPH cord-104269-9r7rqqqk 188 11 label label NN cord-104269-9r7rqqqk 188 12 and and CC cord-104269-9r7rqqqk 188 13 chased chase VBN cord-104269-9r7rqqqk 188 14 for for IN cord-104269-9r7rqqqk 188 15 various various JJ cord-104269-9r7rqqqk 188 16 periods period NNS cord-104269-9r7rqqqk 188 17 of of IN cord-104269-9r7rqqqk 188 18 time time NN cord-104269-9r7rqqqk 188 19 . . . cord-104269-9r7rqqqk 189 1 TRs tr NNS cord-104269-9r7rqqqk 189 2 were be VBD cord-104269-9r7rqqqk 189 3 then then RB cord-104269-9r7rqqqk 189 4 immunoprecipitated immunoprecipitate VBN cord-104269-9r7rqqqk 189 5 from from IN cord-104269-9r7rqqqk 189 6 post post JJ cord-104269-9r7rqqqk 189 7 - - JJ cord-104269-9r7rqqqk 189 8 nuclear nuclear JJ cord-104269-9r7rqqqk 189 9 supernatants supernatant NNS cord-104269-9r7rqqqk 189 10 and and CC cord-104269-9r7rqqqk 189 11 analyzed analyze VBD cord-104269-9r7rqqqk 189 12 on on IN cord-104269-9r7rqqqk 189 13 SDS SDS NNP cord-104269-9r7rqqqk 189 14 - - HYPH cord-104269-9r7rqqqk 189 15 polyacrylamide polyacrylamide NN cord-104269-9r7rqqqk 189 16 gels gel NNS cord-104269-9r7rqqqk 189 17 as as IN cord-104269-9r7rqqqk 189 18 described describe VBN cord-104269-9r7rqqqk 189 19 in in IN cord-104269-9r7rqqqk 189 20 Materials material NNS cord-104269-9r7rqqqk 189 21 and and CC cord-104269-9r7rqqqk 189 22 Methods method NNS cord-104269-9r7rqqqk 189 23 . . . cord-104269-9r7rqqqk 190 1 Dried dry VBN cord-104269-9r7rqqqk 190 2 gels gel NNS cord-104269-9r7rqqqk 190 3 were be VBD cord-104269-9r7rqqqk 190 4 exposed expose VBN cord-104269-9r7rqqqk 190 5 to to IN cord-104269-9r7rqqqk 190 6 pralashed pralashe VBN cord-104269-9r7rqqqk 190 7 XAR XAR NNP cord-104269-9r7rqqqk 190 8 film film NN cord-104269-9r7rqqqk 190 9 for for IN cord-104269-9r7rqqqk 190 10 two two CD cord-104269-9r7rqqqk 190 11 days day NNS cord-104269-9r7rqqqk 190 12 ( ( -LRB- cord-104269-9r7rqqqk 190 13 Eastman Eastman NNP cord-104269-9r7rqqqk 190 14 Kodak Kodak NNP cord-104269-9r7rqqqk 190 15 , , , cord-104269-9r7rqqqk 190 16 Rochester Rochester NNP cord-104269-9r7rqqqk 190 17 , , , cord-104269-9r7rqqqk 190 18 NY NY NNP cord-104269-9r7rqqqk 190 19 ) ) -RRB- cord-104269-9r7rqqqk 190 20 . . . cord-104269-9r7rqqqk 191 1 Immunoprecipitates Immunoprecipitates NNP cord-104269-9r7rqqqk 191 2 were be VBD cord-104269-9r7rqqqk 191 3 quantitated quantitate VBN cord-104269-9r7rqqqk 191 4 on on IN cord-104269-9r7rqqqk 191 5 a a DT cord-104269-9r7rqqqk 191 6 model model NN cord-104269-9r7rqqqk 191 7 425 425 CD cord-104269-9r7rqqqk 191 8 Phos Phos NNP cord-104269-9r7rqqqk 191 9 - - HYPH cord-104269-9r7rqqqk 191 10 phorImager phorimager NN cord-104269-9r7rqqqk 192 1 ( ( -LRB- cord-104269-9r7rqqqk 192 2 Molecular Molecular NNP cord-104269-9r7rqqqk 192 3 Dynamics Dynamics NNPS cord-104269-9r7rqqqk 192 4 ) ) -RRB- cord-104269-9r7rqqqk 192 5 . . . cord-104269-9r7rqqqk 193 1 The the DT cord-104269-9r7rqqqk 193 2 data datum NNS cord-104269-9r7rqqqk 193 3 shown show VBN cord-104269-9r7rqqqk 193 4 are be VBP cord-104269-9r7rqqqk 193 5 from from IN cord-104269-9r7rqqqk 193 6 one one CD cord-104269-9r7rqqqk 193 7 of of IN cord-104269-9r7rqqqk 193 8 two two CD cord-104269-9r7rqqqk 193 9 similar similar JJ cord-104269-9r7rqqqk 193 10 experiments experiment NNS cord-104269-9r7rqqqk 193 11 . . . cord-104269-9r7rqqqk 194 1 chase chase NNP cord-104269-9r7rqqqk 194 2 experiments experiment NNS cord-104269-9r7rqqqk 194 3 were be VBD cord-104269-9r7rqqqk 194 4 performed perform VBN cord-104269-9r7rqqqk 194 5 . . . cord-104269-9r7rqqqk 195 1 CEF CEF NNP cord-104269-9r7rqqqk 195 2 expressing express VBG cord-104269-9r7rqqqk 195 3 either either CC cord-104269-9r7rqqqk 195 4 TR tr NN cord-104269-9r7rqqqk 195 5 , , , cord-104269-9r7rqqqk 195 6 Iicr Iicr NNP cord-104269-9r7rqqqk 195 7 , , , cord-104269-9r7rqqqk 195 8 Iicr+r~ Iicr+r~ NNP cord-104269-9r7rqqqk 195 9 or or CC cord-104269-9r7rqqqk 195 10 the the DT cord-104269-9r7rqqqk 195 11 Iicr Iicr NNP cord-104269-9r7rqqqk 195 12 LI LI NNP cord-104269-9r7rqqqk 195 13 , , , cord-104269-9r7rqqqk 195 14 LP LP NNP cord-104269-9r7rqqqk 195 15 --- --- : cord-104269-9r7rqqqk 195 16 AA AA NNP cord-104269-9r7rqqqk 195 17 , , , cord-104269-9r7rqqqk 195 18 AA AA NNP cord-104269-9r7rqqqk 195 19 mutant mutant NN cord-104269-9r7rqqqk 195 20 were be VBD cord-104269-9r7rqqqk 195 21 pulse pulse NN cord-104269-9r7rqqqk 195 22 - - HYPH cord-104269-9r7rqqqk 195 23 labeled label VBN cord-104269-9r7rqqqk 195 24 with with IN cord-104269-9r7rqqqk 195 25 trans-3sS trans-3ss NN cord-104269-9r7rqqqk 195 26 - - HYPH cord-104269-9r7rqqqk 195 27 label label NN cord-104269-9r7rqqqk 195 28 for for IN cord-104269-9r7rqqqk 195 29 30 30 CD cord-104269-9r7rqqqk 195 30 min min NN cord-104269-9r7rqqqk 195 31 and and CC cord-104269-9r7rqqqk 195 32 chased chase VBN cord-104269-9r7rqqqk 195 33 for for IN cord-104269-9r7rqqqk 195 34 various various JJ cord-104269-9r7rqqqk 195 35 lengths length NNS cord-104269-9r7rqqqk 195 36 of of IN cord-104269-9r7rqqqk 195 37 time time NN cord-104269-9r7rqqqk 195 38 ; ; : cord-104269-9r7rqqqk 195 39 TR TR NNP cord-104269-9r7rqqqk 195 40 and and CC cord-104269-9r7rqqqk 195 41 Ii Ii NNP cord-104269-9r7rqqqk 195 42 - - HYPH cord-104269-9r7rqqqk 195 43 TR TR NNP cord-104269-9r7rqqqk 195 44 chimeras chimera NNS cord-104269-9r7rqqqk 195 45 were be VBD cord-104269-9r7rqqqk 195 46 then then RB cord-104269-9r7rqqqk 195 47 isolated isolate VBN cord-104269-9r7rqqqk 195 48 by by IN cord-104269-9r7rqqqk 195 49 immunoprecipitation immunoprecipitation NN cord-104269-9r7rqqqk 195 50 and and CC cord-104269-9r7rqqqk 195 51 analyzed analyze VBN cord-104269-9r7rqqqk 195 52 by by IN cord-104269-9r7rqqqk 195 53 SDS SDS NNP cord-104269-9r7rqqqk 195 54 - - HYPH cord-104269-9r7rqqqk 195 55 PAGE PAGE NNP cord-104269-9r7rqqqk 195 56 ( ( -LRB- cord-104269-9r7rqqqk 195 57 Fig Fig NNP cord-104269-9r7rqqqk 195 58 . . NNP cord-104269-9r7rqqqk 195 59 2 2 CD cord-104269-9r7rqqqk 195 60 ) ) -RRB- cord-104269-9r7rqqqk 195 61 . . . cord-104269-9r7rqqqk 196 1 Notably notably RB cord-104269-9r7rqqqk 196 2 , , , cord-104269-9r7rqqqk 196 3 both both CC cord-104269-9r7rqqqk 196 4 the the DT cord-104269-9r7rqqqk 196 5 Iicr÷rM Iicr÷rM NNP cord-104269-9r7rqqqk 196 6 and and CC cord-104269-9r7rqqqk 196 7 Iier Iier NNP cord-104269-9r7rqqqk 196 8 chimeras chimera NNS cord-104269-9r7rqqqk 196 9 were be VBD cord-104269-9r7rqqqk 196 10 rapidly rapidly RB cord-104269-9r7rqqqk 196 11 degraded degrade VBN cord-104269-9r7rqqqk 196 12 with with IN cord-104269-9r7rqqqk 196 13 half half RB cord-104269-9r7rqqqk 196 14 - - HYPH cord-104269-9r7rqqqk 196 15 lives life NNS cord-104269-9r7rqqqk 196 16 of of IN cord-104269-9r7rqqqk 196 17 < < NNP cord-104269-9r7rqqqk 196 18 2 2 CD cord-104269-9r7rqqqk 196 19 h h NNP cord-104269-9r7rqqqk 196 20 and and CC cord-104269-9r7rqqqk 196 21 ' ' `` cord-104269-9r7rqqqk 196 22 ~,4 ~,4 CD cord-104269-9r7rqqqk 196 23 h h NN cord-104269-9r7rqqqk 196 24 , , , cord-104269-9r7rqqqk 196 25 respectively respectively RB cord-104269-9r7rqqqk 196 26 , , , cord-104269-9r7rqqqk 196 27 similar similar JJ cord-104269-9r7rqqqk 196 28 to to IN cord-104269-9r7rqqqk 196 29 the the DT cord-104269-9r7rqqqk 196 30 rate rate NN cord-104269-9r7rqqqk 196 31 of of IN cord-104269-9r7rqqqk 196 32 turnover turnover NN cord-104269-9r7rqqqk 196 33 of of IN cord-104269-9r7rqqqk 196 34 native native JJ cord-104269-9r7rqqqk 196 35 Ii Ii NNP cord-104269-9r7rqqqk 196 36 in in IN cord-104269-9r7rqqqk 196 37 antigen antigen NN cord-104269-9r7rqqqk 196 38 - - HYPH cord-104269-9r7rqqqk 196 39 presenting present VBG cord-104269-9r7rqqqk 196 40 cells cell NNS cord-104269-9r7rqqqk 196 41 . . . cord-104269-9r7rqqqk 197 1 In in IN cord-104269-9r7rqqqk 197 2 contrast contrast NN cord-104269-9r7rqqqk 197 3 , , , cord-104269-9r7rqqqk 197 4 the the DT cord-104269-9r7rqqqk 197 5 wild wild JJ cord-104269-9r7rqqqk 197 6 - - HYPH cord-104269-9r7rqqqk 197 7 type type NN cord-104269-9r7rqqqk 197 8 TR tr NN cord-104269-9r7rqqqk 197 9 had have VBD cord-104269-9r7rqqqk 197 10 a a DT cord-104269-9r7rqqqk 197 11 half half JJ cord-104269-9r7rqqqk 197 12 - - HYPH cord-104269-9r7rqqqk 197 13 life life NN cord-104269-9r7rqqqk 197 14 of of IN cord-104269-9r7rqqqk 197 15 ' ' `` cord-104269-9r7rqqqk 197 16 ~24 ~24 IN cord-104269-9r7rqqqk 197 17 h h NN cord-104269-9r7rqqqk 197 18 , , , cord-104269-9r7rqqqk 197 19 consistent consistent JJ cord-104269-9r7rqqqk 197 20 with with IN cord-104269-9r7rqqqk 197 21 previous previous JJ cord-104269-9r7rqqqk 197 22 estimates estimate NNS cord-104269-9r7rqqqk 197 23 of of IN cord-104269-9r7rqqqk 197 24 wild wild JJ cord-104269-9r7rqqqk 197 25 - - HYPH cord-104269-9r7rqqqk 197 26 type type NN cord-104269-9r7rqqqk 197 27 human human JJ cord-104269-9r7rqqqk 197 28 TR tr NN cord-104269-9r7rqqqk 197 29 turnover turnover NN cord-104269-9r7rqqqk 197 30 rates rate NNS cord-104269-9r7rqqqk 197 31 in in IN cord-104269-9r7rqqqk 197 32 CEF CEF NNP cord-104269-9r7rqqqk 197 33 . . . cord-104269-9r7rqqqk 198 1 The the DT cord-104269-9r7rqqqk 198 2 Iicr÷a~ iicr÷a~ CD cord-104269-9r7rqqqk 198 3 chimera chimera NN cord-104269-9r7rqqqk 198 4 was be VBD cord-104269-9r7rqqqk 198 5 consistently consistently RB cord-104269-9r7rqqqk 198 6 degraded degrade VBN cord-104269-9r7rqqqk 198 7 > > XX cord-104269-9r7rqqqk 198 8 2-fold 2-fold CD cord-104269-9r7rqqqk 198 9 more more RBR cord-104269-9r7rqqqk 198 10 rapidly rapidly RB cord-104269-9r7rqqqk 198 11 than than IN cord-104269-9r7rqqqk 198 12 the the DT cord-104269-9r7rqqqk 198 13 Iier Iier NNP cord-104269-9r7rqqqk 198 14 chimera chimera NN cord-104269-9r7rqqqk 198 15 , , , cord-104269-9r7rqqqk 198 16 implying imply VBG cord-104269-9r7rqqqk 198 17 that that IN cord-104269-9r7rqqqk 198 18 the the DT cord-104269-9r7rqqqk 198 19 Ii Ii NNP cord-104269-9r7rqqqk 198 20 transmembrane transmembrane NNP cord-104269-9r7rqqqk 198 21 region region NN cord-104269-9r7rqqqk 198 22 influences influence VBZ cord-104269-9r7rqqqk 198 23 intracellular intracellular JJ cord-104269-9r7rqqqk 198 24 trafficking trafficking NN cord-104269-9r7rqqqk 198 25 . . . cord-104269-9r7rqqqk 199 1 After after IN cord-104269-9r7rqqqk 199 2 2 2 CD cord-104269-9r7rqqqk 199 3 h h NN cord-104269-9r7rqqqk 199 4 ( ( -LRB- cord-104269-9r7rqqqk 199 5 Fig Fig NNP cord-104269-9r7rqqqk 199 6 . . NNP cord-104269-9r7rqqqk 199 7 2 2 CD cord-104269-9r7rqqqk 199 8 ) ) -RRB- cord-104269-9r7rqqqk 199 9 , , , cord-104269-9r7rqqqk 199 10 the the DT cord-104269-9r7rqqqk 199 11 Mr Mr NNP cord-104269-9r7rqqqk 199 12 of of IN cord-104269-9r7rqqqk 199 13 TR TR NNP cord-104269-9r7rqqqk 199 14 and and CC cord-104269-9r7rqqqk 199 15 Ii Ii NNP cord-104269-9r7rqqqk 199 16 - - HYPH cord-104269-9r7rqqqk 199 17 TR TR NNP cord-104269-9r7rqqqk 199 18 chimeras chimera NNS cord-104269-9r7rqqqk 199 19 increased increase VBD cord-104269-9r7rqqqk 199 20 to to IN cord-104269-9r7rqqqk 199 21 that that DT cord-104269-9r7rqqqk 199 22 of of IN cord-104269-9r7rqqqk 199 23 the the DT cord-104269-9r7rqqqk 199 24 mature mature JJ cord-104269-9r7rqqqk 199 25 glycoprotein glycoprotein NN cord-104269-9r7rqqqk 199 26 and and CC cord-104269-9r7rqqqk 199 27 the the DT cord-104269-9r7rqqqk 199 28 molecules molecule NNS cord-104269-9r7rqqqk 199 29 became become VBD cord-104269-9r7rqqqk 199 30 partially partially RB cord-104269-9r7rqqqk 199 31 resistant resistant JJ cord-104269-9r7rqqqk 199 32 to to IN cord-104269-9r7rqqqk 199 33 endoglycosidase endoglycosidase FW cord-104269-9r7rqqqk 199 34 H h NN cord-104269-9r7rqqqk 199 35 digestion digestion NN cord-104269-9r7rqqqk 199 36 ( ( -LRB- cord-104269-9r7rqqqk 199 37 data datum NNS cord-104269-9r7rqqqk 199 38 not not RB cord-104269-9r7rqqqk 199 39 shown show VBN cord-104269-9r7rqqqk 199 40 ) ) -RRB- cord-104269-9r7rqqqk 199 41 . . . cord-104269-9r7rqqqk 200 1 This this DT cord-104269-9r7rqqqk 200 2 indicates indicate VBZ cord-104269-9r7rqqqk 200 3 that that IN cord-104269-9r7rqqqk 200 4 the the DT cord-104269-9r7rqqqk 200 5 Ii Ii NNP cord-104269-9r7rqqqk 200 6 - - HYPH cord-104269-9r7rqqqk 200 7 TR TR NNP cord-104269-9r7rqqqk 200 8 chimeras chimera NNS cord-104269-9r7rqqqk 200 9 traverse traverse VBP cord-104269-9r7rqqqk 200 10 the the DT cord-104269-9r7rqqqk 200 11 Golgi golgi NN cord-104269-9r7rqqqk 200 12 where where WRB cord-104269-9r7rqqqk 200 13 glycosylation glycosylation NN cord-104269-9r7rqqqk 200 14 is be VBZ cord-104269-9r7rqqqk 200 15 completed complete VBN cord-104269-9r7rqqqk 200 16 and and CC cord-104269-9r7rqqqk 200 17 are be VBP cord-104269-9r7rqqqk 200 18 degraded degrade VBN cord-104269-9r7rqqqk 200 19 in in IN cord-104269-9r7rqqqk 200 20 a a DT cord-104269-9r7rqqqk 200 21 post post JJ cord-104269-9r7rqqqk 200 22 - - JJ cord-104269-9r7rqqqk 200 23 Golgi Golgi NNP cord-104269-9r7rqqqk 200 24 compartment compartment NN cord-104269-9r7rqqqk 200 25 . . . cord-104269-9r7rqqqk 201 1 The the DT cord-104269-9r7rqqqk 201 2 Ii Ii NNP cord-104269-9r7rqqqk 201 3 - - HYPH cord-104269-9r7rqqqk 201 4 TR TR NNP cord-104269-9r7rqqqk 201 5 chimera chimera NN cord-104269-9r7rqqqk 201 6 lacking lack VBG cord-104269-9r7rqqqk 201 7 the the DT cord-104269-9r7rqqqk 201 8 non non JJ cord-104269-9r7rqqqk 201 9 - - JJ cord-104269-9r7rqqqk 201 10 tyrosine tyrosine NN cord-104269-9r7rqqqk 201 11 - - HYPH cord-104269-9r7rqqqk 201 12 based base VBN cord-104269-9r7rqqqk 201 13 signals signal NNS cord-104269-9r7rqqqk 201 14 was be VBD cord-104269-9r7rqqqk 201 15 degraded degrade VBN cord-104269-9r7rqqqk 201 16 more more RBR cord-104269-9r7rqqqk 201 17 slowly slowly RB cord-104269-9r7rqqqk 201 18 , , , cord-104269-9r7rqqqk 201 19 indicating indicate VBG cord-104269-9r7rqqqk 201 20 that that IN cord-104269-9r7rqqqk 201 21 these these DT cord-104269-9r7rqqqk 201 22 signals signal NNS cord-104269-9r7rqqqk 201 23 are be VBP cord-104269-9r7rqqqk 201 24 required require VBN cord-104269-9r7rqqqk 201 25 for for IN cord-104269-9r7rqqqk 201 26 delivery delivery NN cord-104269-9r7rqqqk 201 27 to to IN cord-104269-9r7rqqqk 201 28 this this DT cord-104269-9r7rqqqk 201 29 compartment compartment NN cord-104269-9r7rqqqk 201 30 . . . cord-104269-9r7rqqqk 202 1 Further further RB cord-104269-9r7rqqqk 202 2 , , , cord-104269-9r7rqqqk 202 3 the the DT cord-104269-9r7rqqqk 202 4 rate rate NN cord-104269-9r7rqqqk 202 5 of of IN cord-104269-9r7rqqqk 202 6 synthesis synthesis NN cord-104269-9r7rqqqk 202 7 of of IN cord-104269-9r7rqqqk 202 8 the the DT cord-104269-9r7rqqqk 202 9 Iicx Iicx NNP cord-104269-9r7rqqqk 202 10 and and CC cord-104269-9r7rqqqk 202 11 Iicr+m Iicr+m NNP cord-104269-9r7rqqqk 202 12 chimeras chimera NNS cord-104269-9r7rqqqk 202 13 was be VBD cord-104269-9r7rqqqk 202 14 4-and 4-and CD cord-104269-9r7rqqqk 202 15 8-fold 8-fold CD cord-104269-9r7rqqqk 202 16 higher high JJR cord-104269-9r7rqqqk 202 17 , , , cord-104269-9r7rqqqk 202 18 respectively respectively RB cord-104269-9r7rqqqk 202 19 , , , cord-104269-9r7rqqqk 202 20 than than IN cord-104269-9r7rqqqk 202 21 that that DT cord-104269-9r7rqqqk 202 22 of of IN cord-104269-9r7rqqqk 202 23 wild wild JJ cord-104269-9r7rqqqk 202 24 - - HYPH cord-104269-9r7rqqqk 202 25 type type NN cord-104269-9r7rqqqk 202 26 TR tr NN cord-104269-9r7rqqqk 202 27 even even RB cord-104269-9r7rqqqk 202 28 though though IN cord-104269-9r7rqqqk 202 29 the the DT cord-104269-9r7rqqqk 202 30 chimeric chimeric JJ cord-104269-9r7rqqqk 202 31 receptors receptor NNS cord-104269-9r7rqqqk 202 32 were be VBD cord-104269-9r7rqqqk 202 33 expressed express VBN cord-104269-9r7rqqqk 202 34 in in IN cord-104269-9r7rqqqk 202 35 lower low JJR cord-104269-9r7rqqqk 202 36 amounts amount NNS cord-104269-9r7rqqqk 202 37 on on IN cord-104269-9r7rqqqk 202 38 the the DT cord-104269-9r7rqqqk 202 39 cell cell NN cord-104269-9r7rqqqk 202 40 surface surface NN cord-104269-9r7rqqqk 202 41 suggesting suggest VBG cord-104269-9r7rqqqk 202 42 most most JJS cord-104269-9r7rqqqk 202 43 of of IN cord-104269-9r7rqqqk 202 44 the the DT cord-104269-9r7rqqqk 202 45 chimeric chimeric JJ cord-104269-9r7rqqqk 202 46 molecules molecule NNS cord-104269-9r7rqqqk 202 47 were be VBD cord-104269-9r7rqqqk 202 48 trafficking traffic VBG cord-104269-9r7rqqqk 202 49 by by IN cord-104269-9r7rqqqk 202 50 a a DT cord-104269-9r7rqqqk 202 51 direct direct JJ cord-104269-9r7rqqqk 202 52 intracellular intracellular JJ cord-104269-9r7rqqqk 202 53 route route NN cord-104269-9r7rqqqk 202 54 to to IN cord-104269-9r7rqqqk 202 55 the the DT cord-104269-9r7rqqqk 202 56 endocytic endocytic JJ cord-104269-9r7rqqqk 202 57 compartment compartment NN cord-104269-9r7rqqqk 202 58 where where WRB cord-104269-9r7rqqqk 202 59 they -PRON- PRP cord-104269-9r7rqqqk 202 60 were be VBD cord-104269-9r7rqqqk 202 61 degraded degrade VBN cord-104269-9r7rqqqk 202 62 . . . cord-104269-9r7rqqqk 203 1 Degradation degradation NN cord-104269-9r7rqqqk 203 2 of of IN cord-104269-9r7rqqqk 203 3 Ii Ii NNP cord-104269-9r7rqqqk 203 4 is be VBZ cord-104269-9r7rqqqk 203 5 partially partially RB cord-104269-9r7rqqqk 203 6 inhibited inhibit VBN cord-104269-9r7rqqqk 203 7 by by IN cord-104269-9r7rqqqk 203 8 lysosomotropic lysosomotropic JJ cord-104269-9r7rqqqk 203 9 agents agent NNS cord-104269-9r7rqqqk 203 10 such such JJ cord-104269-9r7rqqqk 203 11 as as IN cord-104269-9r7rqqqk 203 12 chloroquine chloroquine NN cord-104269-9r7rqqqk 203 13 , , , cord-104269-9r7rqqqk 203 14 monensin monensin NNP cord-104269-9r7rqqqk 203 15 , , , cord-104269-9r7rqqqk 203 16 and and CC cord-104269-9r7rqqqk 203 17 leupeptin leupeptin NNP cord-104269-9r7rqqqk 203 18 ( ( -LRB- cord-104269-9r7rqqqk 203 19 Ilium Ilium NNP cord-104269-9r7rqqqk 203 20 and and CC cord-104269-9r7rqqqk 203 21 Cresswell Cresswell NNP cord-104269-9r7rqqqk 203 22 , , , cord-104269-9r7rqqqk 203 23 1988 1988 CD cord-104269-9r7rqqqk 203 24 ; ; : cord-104269-9r7rqqqk 203 25 Nguyen Nguyen NNP cord-104269-9r7rqqqk 203 26 and and CC cord-104269-9r7rqqqk 203 27 Humphreys Humphreys NNP cord-104269-9r7rqqqk 203 28 1989 1989 CD cord-104269-9r7rqqqk 203 29 ; ; : cord-104269-9r7rqqqk 203 30 Pieters Pieters NNP cord-104269-9r7rqqqk 203 31 et et NNP cord-104269-9r7rqqqk 203 32 al al NNP cord-104269-9r7rqqqk 203 33 . . NNP cord-104269-9r7rqqqk 203 34 , , , cord-104269-9r7rqqqk 203 35 1991 1991 CD cord-104269-9r7rqqqk 203 36 ; ; : cord-104269-9r7rqqqk 203 37 Zachgo Zachgo NNP cord-104269-9r7rqqqk 203 38 et et NNP cord-104269-9r7rqqqk 203 39 al al NNP cord-104269-9r7rqqqk 203 40 . . NNP cord-104269-9r7rqqqk 203 41 , , , cord-104269-9r7rqqqk 203 42 1992 1992 CD cord-104269-9r7rqqqk 203 43 ) ) -RRB- cord-104269-9r7rqqqk 203 44 . . . cord-104269-9r7rqqqk 204 1 Similarly similarly RB cord-104269-9r7rqqqk 204 2 , , , cord-104269-9r7rqqqk 204 3 leupeptin leupeptin VBZ cord-104269-9r7rqqqk 204 4 significantly significantly RB cord-104269-9r7rqqqk 204 5 inhibited inhibit VBD cord-104269-9r7rqqqk 204 6 the the DT cord-104269-9r7rqqqk 204 7 degradation degradation NN cord-104269-9r7rqqqk 204 8 of of IN cord-104269-9r7rqqqk 204 9 the the DT cord-104269-9r7rqqqk 204 10 Iicr+rM iicr+rm NN cord-104269-9r7rqqqk 204 11 chimera chimera NN cord-104269-9r7rqqqk 204 12 , , , cord-104269-9r7rqqqk 204 13 as as IN cord-104269-9r7rqqqk 204 14 indicated indicate VBN cord-104269-9r7rqqqk 204 15 by by IN cord-104269-9r7rqqqk 204 16 the the DT cord-104269-9r7rqqqk 204 17 slower slow JJR cord-104269-9r7rqqqk 204 18 rate rate NN cord-104269-9r7rqqqk 204 19 of of IN cord-104269-9r7rqqqk 204 20 disappearance disappearance NN cord-104269-9r7rqqqk 204 21 of of IN cord-104269-9r7rqqqk 204 22 the the DT cord-104269-9r7rqqqk 204 23 intact intact JJ cord-104269-9r7rqqqk 204 24 molecule molecule NN cord-104269-9r7rqqqk 204 25 and and CC cord-104269-9r7rqqqk 204 26 the the DT cord-104269-9r7rqqqk 204 27 appearance appearance NN cord-104269-9r7rqqqk 204 28 of of IN cord-104269-9r7rqqqk 204 29 partial partial JJ cord-104269-9r7rqqqk 204 30 proteolytic proteolytic JJ cord-104269-9r7rqqqk 204 31 products product NNS cord-104269-9r7rqqqk 204 32 derived derive VBN cord-104269-9r7rqqqk 204 33 from from IN cord-104269-9r7rqqqk 204 34 the the DT cord-104269-9r7rqqqk 204 35 TR tr NN cord-104269-9r7rqqqk 204 36 external external JJ cord-104269-9r7rqqqk 204 37 domain domain NN cord-104269-9r7rqqqk 204 38 ( ( -LRB- cord-104269-9r7rqqqk 204 39 Fig Fig NNP cord-104269-9r7rqqqk 204 40 . . . cord-104269-9r7rqqqk 204 41 3 3 CD cord-104269-9r7rqqqk 204 42 ) ) -RRB- cord-104269-9r7rqqqk 204 43 , , , cord-104269-9r7rqqqk 204 44 which which WDT cord-104269-9r7rqqqk 204 45 is be VBZ cord-104269-9r7rqqqk 204 46 consistent consistent JJ cord-104269-9r7rqqqk 204 47 with with IN cord-104269-9r7rqqqk 204 48 degradation degradation NN cord-104269-9r7rqqqk 204 49 occurring occur VBG cord-104269-9r7rqqqk 204 50 in in IN cord-104269-9r7rqqqk 204 51 an an DT cord-104269-9r7rqqqk 204 52 endocytic endocytic JJ cord-104269-9r7rqqqk 204 53 compartment compartment NN cord-104269-9r7rqqqk 204 54 . . . cord-104269-9r7rqqqk 205 1 Comparable comparable JJ cord-104269-9r7rqqqk 205 2 results result NNS cord-104269-9r7rqqqk 205 3 were be VBD cord-104269-9r7rqqqk 205 4 obtained obtain VBN cord-104269-9r7rqqqk 205 5 with with IN cord-104269-9r7rqqqk 205 6 chloroquine chloroquine NN cord-104269-9r7rqqqk 205 7 treatment treatment NN cord-104269-9r7rqqqk 205 8 ( ( -LRB- cord-104269-9r7rqqqk 205 9 data datum NNS cord-104269-9r7rqqqk 205 10 not not RB cord-104269-9r7rqqqk 205 11 shown show VBN cord-104269-9r7rqqqk 205 12 ) ) -RRB- cord-104269-9r7rqqqk 205 13 . . . cord-104269-9r7rqqqk 206 1 Ii Ii NNP cord-104269-9r7rqqqk 206 2 - - HYPH cord-104269-9r7rqqqk 206 3 TR TR NNP cord-104269-9r7rqqqk 206 4 chimeras chimera NNS cord-104269-9r7rqqqk 206 5 trafficking traffic VBG cord-104269-9r7rqqqk 206 6 through through IN cord-104269-9r7rqqqk 206 7 the the DT cord-104269-9r7rqqqk 206 8 Golgi Golgi NNP cord-104269-9r7rqqqk 206 9 complex complex NN cord-104269-9r7rqqqk 206 10 were be VBD cord-104269-9r7rqqqk 206 11 identified identify VBN cord-104269-9r7rqqqk 206 12 using use VBG cord-104269-9r7rqqqk 206 13 electron electron NN cord-104269-9r7rqqqk 206 14 microscopy microscopy NNP cord-104269-9r7rqqqk 206 15 immunocytochemistry immunocytochemistry NN cord-104269-9r7rqqqk 206 16 . . . cord-104269-9r7rqqqk 207 1 Gold gold NN cord-104269-9r7rqqqk 207 2 complexes complex NNS cord-104269-9r7rqqqk 207 3 bearing bear VBG cord-104269-9r7rqqqk 207 4 antibody antibody NN cord-104269-9r7rqqqk 207 5 specific specific JJ cord-104269-9r7rqqqk 207 6 for for IN cord-104269-9r7rqqqk 207 7 the the DT cord-104269-9r7rqqqk 207 8 external external JJ cord-104269-9r7rqqqk 207 9 domain domain NN cord-104269-9r7rqqqk 207 10 of of IN cord-104269-9r7rqqqk 207 11 TR TR NNP cord-104269-9r7rqqqk 207 12 were be VBD cord-104269-9r7rqqqk 207 13 applied apply VBN cord-104269-9r7rqqqk 207 14 to to IN cord-104269-9r7rqqqk 207 15 cryosections cryosection NNS cord-104269-9r7rqqqk 207 16 and and CC cord-104269-9r7rqqqk 207 17 showed show VBD cord-104269-9r7rqqqk 207 18 Ii Ii NNP cord-104269-9r7rqqqk 207 19 - - HYPH cord-104269-9r7rqqqk 207 20 TR TR NNP cord-104269-9r7rqqqk 207 21 distributed distribute VBN cord-104269-9r7rqqqk 207 22 throughout throughout IN cord-104269-9r7rqqqk 207 23 the the DT cord-104269-9r7rqqqk 207 24 flattened flatten VBN cord-104269-9r7rqqqk 207 25 cisternae cisternae NN cord-104269-9r7rqqqk 207 26 of of IN cord-104269-9r7rqqqk 207 27 the the DT cord-104269-9r7rqqqk 207 28 Golgi Golgi NNP cord-104269-9r7rqqqk 207 29 stack stack NN cord-104269-9r7rqqqk 207 30 and and CC cord-104269-9r7rqqqk 207 31 within within IN cord-104269-9r7rqqqk 207 32 coated coated JJ cord-104269-9r7rqqqk 207 33 vesicles vesicle NNS cord-104269-9r7rqqqk 207 34 in in IN cord-104269-9r7rqqqk 207 35 the the DT cord-104269-9r7rqqqk 207 36 trans trans NNP cord-104269-9r7rqqqk 207 37 - - NNP cord-104269-9r7rqqqk 207 38 Golgi Golgi NNP cord-104269-9r7rqqqk 207 39 area area NN cord-104269-9r7rqqqk 207 40 ( ( -LRB- cord-104269-9r7rqqqk 207 41 see see VB cord-104269-9r7rqqqk 207 42 Fig Fig NNP cord-104269-9r7rqqqk 207 43 . . . cord-104269-9r7rqqqk 207 44 6 6 CD cord-104269-9r7rqqqk 207 45 ) ) -RRB- cord-104269-9r7rqqqk 207 46 . . . cord-104269-9r7rqqqk 208 1 The the DT cord-104269-9r7rqqqk 208 2 intracellular intracellular JJ cord-104269-9r7rqqqk 208 3 and and CC cord-104269-9r7rqqqk 208 4 cell cell NN cord-104269-9r7rqqqk 208 5 surface surface NN cord-104269-9r7rqqqk 208 6 distribution distribution NN cord-104269-9r7rqqqk 208 7 of of IN cord-104269-9r7rqqqk 208 8 the the DT cord-104269-9r7rqqqk 208 9 Ii Ii NNP cord-104269-9r7rqqqk 208 10 - - HYPH cord-104269-9r7rqqqk 208 11 TR TR NNP cord-104269-9r7rqqqk 208 12 chimeras chimera NNS cord-104269-9r7rqqqk 208 13 was be VBD cord-104269-9r7rqqqk 208 14 determined determine VBN cord-104269-9r7rqqqk 208 15 by by IN cord-104269-9r7rqqqk 208 16 immunofluorescence immunofluorescence NN cord-104269-9r7rqqqk 208 17 microscopy microscopy NN cord-104269-9r7rqqqk 208 18 . . . cord-104269-9r7rqqqk 209 1 The the DT cord-104269-9r7rqqqk 209 2 Iicr+rM iicr+rm NN cord-104269-9r7rqqqk 209 3 chimera chimera NN cord-104269-9r7rqqqk 209 4 was be VBD cord-104269-9r7rqqqk 209 5 found find VBN cord-104269-9r7rqqqk 209 6 predominantly predominantly RB cord-104269-9r7rqqqk 209 7 in in IN cord-104269-9r7rqqqk 209 8 intracellular intracellular JJ cord-104269-9r7rqqqk 209 9 vesicles vesicle NNS cord-104269-9r7rqqqk 209 10 , , , cord-104269-9r7rqqqk 209 11 as as IN cord-104269-9r7rqqqk 209 12 indicated indicate VBN cord-104269-9r7rqqqk 209 13 by by IN cord-104269-9r7rqqqk 209 14 the the DT cord-104269-9r7rqqqk 209 15 virtual virtual JJ cord-104269-9r7rqqqk 209 16 lack lack NN cord-104269-9r7rqqqk 209 17 of of IN cord-104269-9r7rqqqk 209 18 staining staining NN cord-104269-9r7rqqqk 209 19 of of IN cord-104269-9r7rqqqk 209 20 non non JJ cord-104269-9r7rqqqk 209 21 - - JJ cord-104269-9r7rqqqk 209 22 permeabilized permeabilized JJ cord-104269-9r7rqqqk 209 23 cells cell NNS cord-104269-9r7rqqqk 209 24 ( ( -LRB- cord-104269-9r7rqqqk 209 25 Fig Fig NNP cord-104269-9r7rqqqk 209 26 . . . cord-104269-9r7rqqqk 209 27 4 4 CD cord-104269-9r7rqqqk 209 28 A a CD cord-104269-9r7rqqqk 209 29 ) ) -RRB- cord-104269-9r7rqqqk 209 30 , , , cord-104269-9r7rqqqk 209 31 compared compare VBN cord-104269-9r7rqqqk 209 32 to to IN cord-104269-9r7rqqqk 209 33 the the DT cord-104269-9r7rqqqk 209 34 strong strong JJ cord-104269-9r7rqqqk 209 35 punctate punctate NN cord-104269-9r7rqqqk 209 36 staining staining NN cord-104269-9r7rqqqk 209 37 of of IN cord-104269-9r7rqqqk 209 38 permeabilized permeabilized JJ cord-104269-9r7rqqqk 209 39 cells cell NNS cord-104269-9r7rqqqk 209 40 ( ( -LRB- cord-104269-9r7rqqqk 209 41 Fig Fig NNP cord-104269-9r7rqqqk 209 42 . . . cord-104269-9r7rqqqk 209 43 4 4 CD cord-104269-9r7rqqqk 209 44 B b NN cord-104269-9r7rqqqk 209 45 ) ) -RRB- cord-104269-9r7rqqqk 209 46 . . . cord-104269-9r7rqqqk 210 1 CEF CEF NNP cord-104269-9r7rqqqk 210 2 expressing express VBG cord-104269-9r7rqqqk 210 3 the the DT cord-104269-9r7rqqqk 210 4 Iicr Iicr NNP cord-104269-9r7rqqqk 210 5 chimera chimera NN cord-104269-9r7rqqqk 210 6 exhibited exhibit VBD cord-104269-9r7rqqqk 210 7 more more JJR cord-104269-9r7rqqqk 210 8 surface surface NN cord-104269-9r7rqqqk 210 9 staining staining NN cord-104269-9r7rqqqk 210 10 ( ( -LRB- cord-104269-9r7rqqqk 210 11 Fig fig NN cord-104269-9r7rqqqk 210 12 . . . cord-104269-9r7rqqqk 210 13 4 4 CD cord-104269-9r7rqqqk 210 14 E e NN cord-104269-9r7rqqqk 210 15 ) ) -RRB- cord-104269-9r7rqqqk 210 16 , , , cord-104269-9r7rqqqk 210 17 but but CC cord-104269-9r7rqqqk 210 18 this this DT cord-104269-9r7rqqqk 210 19 chimera chimera NN cord-104269-9r7rqqqk 210 20 was be VBD cord-104269-9r7rqqqk 210 21 also also RB cord-104269-9r7rqqqk 210 22 found find VBN cord-104269-9r7rqqqk 210 23 predominantly predominantly RB cord-104269-9r7rqqqk 210 24 in in IN cord-104269-9r7rqqqk 210 25 intracellular intracellular JJ cord-104269-9r7rqqqk 210 26 vesicles vesicle NNS cord-104269-9r7rqqqk 210 27 ( ( -LRB- cord-104269-9r7rqqqk 210 28 Fig fig NN cord-104269-9r7rqqqk 210 29 . . . cord-104269-9r7rqqqk 210 30 4 4 CD cord-104269-9r7rqqqk 210 31 F F NNP cord-104269-9r7rqqqk 210 32 ) ) -RRB- cord-104269-9r7rqqqk 210 33 . . . cord-104269-9r7rqqqk 211 1 The the DT cord-104269-9r7rqqqk 211 2 Iicr Iicr NNP cord-104269-9r7rqqqk 211 3 LI LI NNP cord-104269-9r7rqqqk 211 4 , , , cord-104269-9r7rqqqk 211 5 LP LP NNP cord-104269-9r7rqqqk 211 6 ~ ~ NNP cord-104269-9r7rqqqk 211 7 AA AA NNP cord-104269-9r7rqqqk 211 8 , , , cord-104269-9r7rqqqk 211 9 AA AA NNP cord-104269-9r7rqqqk 211 10 chimera chimera NN cord-104269-9r7rqqqk 211 11 was be VBD cord-104269-9r7rqqqk 211 12 expressed express VBN cord-104269-9r7rqqqk 211 13 mainly mainly RB cord-104269-9r7rqqqk 211 14 on on IN cord-104269-9r7rqqqk 211 15 the the DT cord-104269-9r7rqqqk 211 16 cell cell NN cord-104269-9r7rqqqk 211 17 surface surface NN cord-104269-9r7rqqqk 211 18 ( ( -LRB- cord-104269-9r7rqqqk 211 19 Fig fig NN cord-104269-9r7rqqqk 211 20 . . . cord-104269-9r7rqqqk 211 21 4 4 CD cord-104269-9r7rqqqk 211 22 G G NNP cord-104269-9r7rqqqk 211 23 ) ) -RRB- cord-104269-9r7rqqqk 211 24 , , , cord-104269-9r7rqqqk 211 25 but but CC cord-104269-9r7rqqqk 211 26 some some DT cord-104269-9r7rqqqk 211 27 staining staining NN cord-104269-9r7rqqqk 211 28 of of IN cord-104269-9r7rqqqk 211 29 intracellular intracellular JJ cord-104269-9r7rqqqk 211 30 vesicles vesicle NNS cord-104269-9r7rqqqk 211 31 was be VBD cord-104269-9r7rqqqk 211 32 still still RB cord-104269-9r7rqqqk 211 33 observed observe VBN cord-104269-9r7rqqqk 211 34 ( ( -LRB- cord-104269-9r7rqqqk 211 35 Fig Fig NNP cord-104269-9r7rqqqk 211 36 . . . cord-104269-9r7rqqqk 211 37 4 4 CD cord-104269-9r7rqqqk 211 38 H h NN cord-104269-9r7rqqqk 211 39 ) ) -RRB- cord-104269-9r7rqqqk 211 40 . . . cord-104269-9r7rqqqk 212 1 Two two CD cord-104269-9r7rqqqk 212 2 - - HYPH cord-104269-9r7rqqqk 212 3 color color NN cord-104269-9r7rqqqk 212 4 immunofluorescence immunofluorescence NN cord-104269-9r7rqqqk 212 5 studies study NNS cord-104269-9r7rqqqk 212 6 comparing compare VBG cord-104269-9r7rqqqk 212 7 the the DT cord-104269-9r7rqqqk 212 8 intracellular intracellular JJ cord-104269-9r7rqqqk 212 9 distribution distribution NN cord-104269-9r7rqqqk 212 10 of of IN cord-104269-9r7rqqqk 212 11 the the DT cord-104269-9r7rqqqk 212 12 Iicr+rM iicr+rm NN cord-104269-9r7rqqqk 212 13 chimera chimera NN cord-104269-9r7rqqqk 212 14 with with IN cord-104269-9r7rqqqk 212 15 LEP100 LEP100 NNPS cord-104269-9r7rqqqk 212 16 , , , cord-104269-9r7rqqqk 212 17 an an DT cord-104269-9r7rqqqk 212 18 endogenous endogenous JJ cord-104269-9r7rqqqk 212 19 chicken chicken NN cord-104269-9r7rqqqk 212 20 lysosomal lysosomal NN cord-104269-9r7rqqqk 212 21 integral integral JJ cord-104269-9r7rqqqk 212 22 membrane membrane NN cord-104269-9r7rqqqk 212 23 protein protein NN cord-104269-9r7rqqqk 212 24 Fambrough Fambrough NNP cord-104269-9r7rqqqk 212 25 , , , cord-104269-9r7rqqqk 212 26 1986 1986 CD cord-104269-9r7rqqqk 212 27 , , , cord-104269-9r7rqqqk 212 28 1987 1987 CD cord-104269-9r7rqqqk 212 29 ; ; : cord-104269-9r7rqqqk 213 1 Mathews Mathews NNP cord-104269-9r7rqqqk 213 2 et et NNP cord-104269-9r7rqqqk 213 3 al al NNP cord-104269-9r7rqqqk 213 4 . . NNP cord-104269-9r7rqqqk 213 5 , , , cord-104269-9r7rqqqk 213 6 1992 1992 CD cord-104269-9r7rqqqk 213 7 ) ) -RRB- cord-104269-9r7rqqqk 213 8 , , , cord-104269-9r7rqqqk 213 9 showed show VBD cord-104269-9r7rqqqk 213 10 significant significant JJ cord-104269-9r7rqqqk 213 11 colocalization colocalization NN cord-104269-9r7rqqqk 213 12 , , , cord-104269-9r7rqqqk 213 13 indicating indicate VBG cord-104269-9r7rqqqk 213 14 that that IN cord-104269-9r7rqqqk 213 15 the the DT cord-104269-9r7rqqqk 213 16 hybrid hybrid JJ cord-104269-9r7rqqqk 213 17 molecules molecule NNS cord-104269-9r7rqqqk 213 18 traffic traffic NN cord-104269-9r7rqqqk 213 19 along along IN cord-104269-9r7rqqqk 213 20 the the DT cord-104269-9r7rqqqk 213 21 prelysosomal prelysosomal JJ cord-104269-9r7rqqqk 213 22 segment segment NN cord-104269-9r7rqqqk 213 23 of of IN cord-104269-9r7rqqqk 213 24 the the DT cord-104269-9r7rqqqk 213 25 endocytic endocytic JJ cord-104269-9r7rqqqk 213 26 pathway pathway NN cord-104269-9r7rqqqk 213 27 ( ( -LRB- cord-104269-9r7rqqqk 213 28 Fig Fig NNP cord-104269-9r7rqqqk 213 29 . . . cord-104269-9r7rqqqk 213 30 5 5 CD cord-104269-9r7rqqqk 213 31 C C NNP cord-104269-9r7rqqqk 213 32 ) ) -RRB- cord-104269-9r7rqqqk 213 33 . . . cord-104269-9r7rqqqk 214 1 Conversely conversely RB cord-104269-9r7rqqqk 214 2 , , , cord-104269-9r7rqqqk 214 3 wild wild JJ cord-104269-9r7rqqqk 214 4 - - HYPH cord-104269-9r7rqqqk 214 5 type type NN cord-104269-9r7rqqqk 214 6 TR tr NN cord-104269-9r7rqqqk 214 7 showed show VBD cord-104269-9r7rqqqk 214 8 virtually virtually RB cord-104269-9r7rqqqk 214 9 no no DT cord-104269-9r7rqqqk 214 10 colocalization colocalization NN cord-104269-9r7rqqqk 214 11 with with IN cord-104269-9r7rqqqk 214 12 LEP100 LEP100 NNPS cord-104269-9r7rqqqk 215 1 ( ( -LRB- cord-104269-9r7rqqqk 215 2 Fig Fig NNP cord-104269-9r7rqqqk 215 3 . . . cord-104269-9r7rqqqk 215 4 5 5 CD cord-104269-9r7rqqqk 215 5 A a NN cord-104269-9r7rqqqk 215 6 ) ) -RRB- cord-104269-9r7rqqqk 215 7 . . . cord-104269-9r7rqqqk 216 1 The the DT cord-104269-9r7rqqqk 216 2 Iicv Iicv NNP cord-104269-9r7rqqqk 216 3 chimera chimera NN cord-104269-9r7rqqqk 216 4 also also RB cord-104269-9r7rqqqk 216 5 partially partially RB cord-104269-9r7rqqqk 216 6 colocalized colocalize VBN cord-104269-9r7rqqqk 216 7 with with IN cord-104269-9r7rqqqk 216 8 LEP100 LEP100 NNS cord-104269-9r7rqqqk 216 9 ( ( -LRB- cord-104269-9r7rqqqk 216 10 Fig fig NN cord-104269-9r7rqqqk 216 11 . . . cord-104269-9r7rqqqk 216 12 5 5 CD cord-104269-9r7rqqqk 216 13 B b NN cord-104269-9r7rqqqk 216 14 ) ) -RRB- cord-104269-9r7rqqqk 216 15 , , , cord-104269-9r7rqqqk 216 16 whereas whereas IN cord-104269-9r7rqqqk 216 17 the the DT cord-104269-9r7rqqqk 216 18 Iicr Iicr NNP cord-104269-9r7rqqqk 216 19 LI LI NNP cord-104269-9r7rqqqk 216 20 , , , cord-104269-9r7rqqqk 216 21 LP-*AA LP-*AA NNP cord-104269-9r7rqqqk 216 22 , , , cord-104269-9r7rqqqk 216 23 AA AA NNP cord-104269-9r7rqqqk 216 24 ( ( -LRB- cord-104269-9r7rqqqk 216 25 Fig Fig NNP cord-104269-9r7rqqqk 216 26 . . . cord-104269-9r7rqqqk 216 27 5 5 CD cord-104269-9r7rqqqk 216 28 D d NN cord-104269-9r7rqqqk 216 29 ) ) -RRB- cord-104269-9r7rqqqk 216 30 gave give VBD cord-104269-9r7rqqqk 216 31 a a DT cord-104269-9r7rqqqk 216 32 staining staining NN cord-104269-9r7rqqqk 216 33 pattern pattern NN cord-104269-9r7rqqqk 216 34 similar similar JJ cord-104269-9r7rqqqk 216 35 to to IN cord-104269-9r7rqqqk 216 36 that that DT cord-104269-9r7rqqqk 216 37 of of IN cord-104269-9r7rqqqk 216 38 wild wild JJ cord-104269-9r7rqqqk 216 39 - - HYPH cord-104269-9r7rqqqk 216 40 type type NN cord-104269-9r7rqqqk 216 41 TR tr NN cord-104269-9r7rqqqk 216 42 . . . cord-104269-9r7rqqqk 217 1 To to TO cord-104269-9r7rqqqk 217 2 quantitatively quantitatively RB cord-104269-9r7rqqqk 217 3 determine determine VB cord-104269-9r7rqqqk 217 4 the the DT cord-104269-9r7rqqqk 217 5 altered alter VBN cord-104269-9r7rqqqk 217 6 trafficking trafficking NN cord-104269-9r7rqqqk 217 7 of of IN cord-104269-9r7rqqqk 217 8 Iicr÷m Iicr÷m NNP cord-104269-9r7rqqqk 217 9 chimera chimera NN cord-104269-9r7rqqqk 217 10 , , , cord-104269-9r7rqqqk 217 11 the the DT cord-104269-9r7rqqqk 217 12 steady steady JJ cord-104269-9r7rqqqk 217 13 - - HYPH cord-104269-9r7rqqqk 217 14 state state NN cord-104269-9r7rqqqk 217 15 distribution distribution NN cord-104269-9r7rqqqk 217 16 of of IN cord-104269-9r7rqqqk 217 17 TR TR NNP cord-104269-9r7rqqqk 217 18 and and CC cord-104269-9r7rqqqk 217 19 Ii Ii NNP cord-104269-9r7rqqqk 217 20 - - HYPH cord-104269-9r7rqqqk 217 21 TR TR NNP cord-104269-9r7rqqqk 217 22 chimeras chimera NNS cord-104269-9r7rqqqk 217 23 was be VBD cord-104269-9r7rqqqk 217 24 determined determine VBN cord-104269-9r7rqqqk 217 25 by by IN cord-104269-9r7rqqqk 217 26 gold gold NN cord-104269-9r7rqqqk 217 27 label label NN cord-104269-9r7rqqqk 217 28 immunocytochemistry immunocytochemistry NN cord-104269-9r7rqqqk 217 29 using use VBG cord-104269-9r7rqqqk 217 30 antibody antibody NN cord-104269-9r7rqqqk 217 31 specific specific JJ cord-104269-9r7rqqqk 217 32 for for IN cord-104269-9r7rqqqk 217 33 the the DT cord-104269-9r7rqqqk 217 34 external external JJ cord-104269-9r7rqqqk 217 35 domain domain NN cord-104269-9r7rqqqk 217 36 of of IN cord-104269-9r7rqqqk 217 37 human human JJ cord-104269-9r7rqqqk 217 38 TR tr NN cord-104269-9r7rqqqk 217 39 . . . cord-104269-9r7rqqqk 218 1 The the DT cord-104269-9r7rqqqk 218 2 extent extent NN cord-104269-9r7rqqqk 218 3 to to TO cord-104269-9r7rqqqk 218 4 which which WDT cord-104269-9r7rqqqk 218 5 the the DT cord-104269-9r7rqqqk 218 6 TR TR NNP cord-104269-9r7rqqqk 218 7 and and CC cord-104269-9r7rqqqk 218 8 Iicr+m Iicr+m NNP cord-104269-9r7rqqqk 218 9 chimera chimera NN cord-104269-9r7rqqqk 218 10 were be VBD cord-104269-9r7rqqqk 218 11 colocalized colocalize VBN cord-104269-9r7rqqqk 218 12 with with IN cord-104269-9r7rqqqk 218 13 LEP100 LEP100 NNPS cord-104269-9r7rqqqk 218 14 , , , cord-104269-9r7rqqqk 218 15 an an DT cord-104269-9r7rqqqk 218 16 antigen antigen NN cord-104269-9r7rqqqk 218 17 located locate VBN cord-104269-9r7rqqqk 218 18 predominantly predominantly RB cord-104269-9r7rqqqk 218 19 in in IN cord-104269-9r7rqqqk 218 20 the the DT cord-104269-9r7rqqqk 218 21 prelysosomal prelysosomal JJ cord-104269-9r7rqqqk 218 22 branch branch NN cord-104269-9r7rqqqk 218 23 of of IN cord-104269-9r7rqqqk 218 24 the the DT cord-104269-9r7rqqqk 218 25 endocytic endocytic JJ cord-104269-9r7rqqqk 218 26 pathway pathway NN cord-104269-9r7rqqqk 218 27 Fambrough Fambrough NNP cord-104269-9r7rqqqk 218 28 , , , cord-104269-9r7rqqqk 218 29 1986 1986 CD cord-104269-9r7rqqqk 218 30 , , , cord-104269-9r7rqqqk 218 31 1987 1987 CD cord-104269-9r7rqqqk 218 32 ) ) -RRB- cord-104269-9r7rqqqk 218 33 , , , cord-104269-9r7rqqqk 218 34 was be VBD cord-104269-9r7rqqqk 218 35 quantified quantify VBN cord-104269-9r7rqqqk 218 36 by by IN cord-104269-9r7rqqqk 218 37 double double JJ cord-104269-9r7rqqqk 218 38 labeling labeling NN cord-104269-9r7rqqqk 218 39 . . . cord-104269-9r7rqqqk 219 1 As as IN cord-104269-9r7rqqqk 219 2 shown show VBN cord-104269-9r7rqqqk 219 3 in in IN cord-104269-9r7rqqqk 219 4 Fig Fig NNP cord-104269-9r7rqqqk 219 5 . . NNP cord-104269-9r7rqqqk 219 6 6 6 CD cord-104269-9r7rqqqk 219 7 , , , cord-104269-9r7rqqqk 219 8 the the DT cord-104269-9r7rqqqk 219 9 distribution distribution NN cord-104269-9r7rqqqk 219 10 of of IN cord-104269-9r7rqqqk 219 11 TR tr NN cord-104269-9r7rqqqk 219 12 , , , cord-104269-9r7rqqqk 219 13 Iicr÷m Iicr÷m NNP cord-104269-9r7rqqqk 219 14 , , , cord-104269-9r7rqqqk 219 15 and and CC cord-104269-9r7rqqqk 219 16 LEP100 LEP100 NNPS cord-104269-9r7rqqqk 219 17 is be VBZ cord-104269-9r7rqqqk 219 18 clearly clearly RB cord-104269-9r7rqqqk 219 19 restricted restrict VBN cord-104269-9r7rqqqk 219 20 to to IN cord-104269-9r7rqqqk 219 21 the the DT cord-104269-9r7rqqqk 219 22 perimeter perimeter NN cord-104269-9r7rqqqk 219 23 membranes membrane NNS cord-104269-9r7rqqqk 219 24 of of IN cord-104269-9r7rqqqk 219 25 endocytic endocytic JJ cord-104269-9r7rqqqk 219 26 elements element NNS cord-104269-9r7rqqqk 219 27 . . . cord-104269-9r7rqqqk 220 1 Quantitative quantitative JJ cord-104269-9r7rqqqk 220 2 analysis analysis NN cord-104269-9r7rqqqk 220 3 shows show VBZ cord-104269-9r7rqqqk 220 4 that that IN cord-104269-9r7rqqqk 220 5 while while IN cord-104269-9r7rqqqk 220 6 relatively relatively RB cord-104269-9r7rqqqk 220 7 low low JJ cord-104269-9r7rqqqk 220 8 amounts amount NNS cord-104269-9r7rqqqk 220 9 ( ( -LRB- cord-104269-9r7rqqqk 220 10 11.2 11.2 CD cord-104269-9r7rqqqk 220 11 % % NN cord-104269-9r7rqqqk 220 12 ) ) -RRB- cord-104269-9r7rqqqk 220 13 of of IN cord-104269-9r7rqqqk 220 14 TR TR NNP cord-104269-9r7rqqqk 220 15 can can MD cord-104269-9r7rqqqk 220 16 be be VB cord-104269-9r7rqqqk 220 17 found find VBN cord-104269-9r7rqqqk 220 18 in in IN cord-104269-9r7rqqqk 220 19 LEP100-containing lep100-containe VBG cord-104269-9r7rqqqk 220 20 compartments compartment NNS cord-104269-9r7rqqqk 220 21 , , , cord-104269-9r7rqqqk 220 22 64 64 CD cord-104269-9r7rqqqk 220 23 % % NN cord-104269-9r7rqqqk 220 24 of of IN cord-104269-9r7rqqqk 220 25 the the DT cord-104269-9r7rqqqk 220 26 labeled label VBN cord-104269-9r7rqqqk 220 27 compartments compartment NNS cord-104269-9r7rqqqk 220 28 in in IN cord-104269-9r7rqqqk 220 29 cells cell NNS cord-104269-9r7rqqqk 220 30 transfected transfecte VBN cord-104269-9r7rqqqk 220 31 with with IN cord-104269-9r7rqqqk 220 32 Iicr+~M Iicr+~M NNP cord-104269-9r7rqqqk 220 33 contain contain VBP cord-104269-9r7rqqqk 220 34 both both CC cord-104269-9r7rqqqk 220 35 Iic~÷~ Iic~÷~ NNP cord-104269-9r7rqqqk 220 36 and and CC cord-104269-9r7rqqqk 220 37 LEP100 LEP100 NNP cord-104269-9r7rqqqk 220 38 . . . cord-104269-9r7rqqqk 221 1 To to TO cord-104269-9r7rqqqk 221 2 determine determine VB cord-104269-9r7rqqqk 221 3 whether whether IN cord-104269-9r7rqqqk 221 4 fi fi NN cord-104269-9r7rqqqk 221 5 - - HYPH cord-104269-9r7rqqqk 221 6 TR tr NN cord-104269-9r7rqqqk 221 7 chimeras chimera NNS cord-104269-9r7rqqqk 221 8 expressed express VBD cord-104269-9r7rqqqk 221 9 on on IN cord-104269-9r7rqqqk 221 10 the the DT cord-104269-9r7rqqqk 221 11 cell cell NN cord-104269-9r7rqqqk 221 12 surface surface NN cord-104269-9r7rqqqk 221 13 traffic traffic NN cord-104269-9r7rqqqk 221 14 to to IN cord-104269-9r7rqqqk 221 15 the the DT cord-104269-9r7rqqqk 221 16 endocytic endocytic JJ cord-104269-9r7rqqqk 221 17 compartment compartment NN cord-104269-9r7rqqqk 221 18 where where WRB cord-104269-9r7rqqqk 221 19 degradation degradation NN cord-104269-9r7rqqqk 221 20 occurs occur VBZ cord-104269-9r7rqqqk 221 21 , , , cord-104269-9r7rqqqk 221 22 cells cell NNS cord-104269-9r7rqqqk 221 23 were be VBD cord-104269-9r7rqqqk 221 24 incubated incubate VBN cord-104269-9r7rqqqk 221 25 with with IN cord-104269-9r7rqqqk 221 26 1 1 CD cord-104269-9r7rqqqk 221 27 = = NN cord-104269-9r7rqqqk 221 28 I i NN cord-104269-9r7rqqqk 221 29 - - HYPH cord-104269-9r7rqqqk 221 30 labeled label VBN cord-104269-9r7rqqqk 221 31 Tf Tf NNP cord-104269-9r7rqqqk 221 32 at at IN cord-104269-9r7rqqqk 221 33 37 37 CD cord-104269-9r7rqqqk 221 34 ° ° NN cord-104269-9r7rqqqk 221 35 C c NN cord-104269-9r7rqqqk 221 36 for for IN cord-104269-9r7rqqqk 221 37 1 1 CD cord-104269-9r7rqqqk 221 38 h h NN cord-104269-9r7rqqqk 221 39 to to TO cord-104269-9r7rqqqk 221 40 load load VB cord-104269-9r7rqqqk 221 41 the the DT cord-104269-9r7rqqqk 221 42 endocytic endocytic JJ cord-104269-9r7rqqqk 221 43 pathway pathway NN cord-104269-9r7rqqqk 221 44 with with IN cord-104269-9r7rqqqk 221 45 receptorligand receptorligand NN cord-104269-9r7rqqqk 221 46 complexes complex NNS cord-104269-9r7rqqqk 221 47 . . . cord-104269-9r7rqqqk 222 1 The the DT cord-104269-9r7rqqqk 222 2 cells cell NNS cord-104269-9r7rqqqk 222 3 were be VBD cord-104269-9r7rqqqk 222 4 then then RB cord-104269-9r7rqqqk 222 5 rapidly rapidly RB cord-104269-9r7rqqqk 222 6 washed wash VBN cord-104269-9r7rqqqk 222 7 , , , cord-104269-9r7rqqqk 222 8 and and CC cord-104269-9r7rqqqk 222 9 the the DT cord-104269-9r7rqqqk 222 10 reappearance reappearance NN cord-104269-9r7rqqqk 222 11 of of IN cord-104269-9r7rqqqk 222 12 intact intact JJ cord-104269-9r7rqqqk 222 13 and and CC cord-104269-9r7rqqqk 222 14 degraded degrade VBD cord-104269-9r7rqqqk 222 15 Tf Tf NNP cord-104269-9r7rqqqk 222 16 in in IN cord-104269-9r7rqqqk 222 17 the the DT cord-104269-9r7rqqqk 222 18 medium medium JJ cord-104269-9r7rqqqk 222 19 Time time NN cord-104269-9r7rqqqk 222 20 ( ( -LRB- cord-104269-9r7rqqqk 222 21 min min NNP cord-104269-9r7rqqqk 222 22 ) ) -RRB- cord-104269-9r7rqqqk 222 23 Figure figure NN cord-104269-9r7rqqqk 222 24 7 7 CD cord-104269-9r7rqqqk 222 25 . . . cord-104269-9r7rqqqk 223 1 Degradation degradation NN cord-104269-9r7rqqqk 223 2 of of IN cord-104269-9r7rqqqk 223 3 1 1 CD cord-104269-9r7rqqqk 223 4 T t NN cord-104269-9r7rqqqk 223 5 bound bind VBN cord-104269-9r7rqqqk 223 6 to to IN cord-104269-9r7rqqqk 223 7 Ii Ii NNP cord-104269-9r7rqqqk 223 8 - - HYPH cord-104269-9r7rqqqk 223 9 TR TR NNP cord-104269-9r7rqqqk 223 10 chimeras chimera NNS cord-104269-9r7rqqqk 223 11 . . . cord-104269-9r7rqqqk 224 1 Equivalent equivalent JJ cord-104269-9r7rqqqk 224 2 cell cell NN cord-104269-9r7rqqqk 224 3 numbers number NNS cord-104269-9r7rqqqk 224 4 of of IN cord-104269-9r7rqqqk 224 5 CEF CEF NNP cord-104269-9r7rqqqk 224 6 expressing express VBG cord-104269-9r7rqqqk 224 7 either either CC cord-104269-9r7rqqqk 224 8 wild wild JJ cord-104269-9r7rqqqk 224 9 - - HYPH cord-104269-9r7rqqqk 224 10 type type NN cord-104269-9r7rqqqk 224 11 TR tr NN cord-104269-9r7rqqqk 224 12 , , , cord-104269-9r7rqqqk 224 13 Iicr Iicr NNP cord-104269-9r7rqqqk 224 14 , , , cord-104269-9r7rqqqk 224 15 IicT+m iict+m CD cord-104269-9r7rqqqk 224 16 , , , cord-104269-9r7rqqqk 224 17 or or CC cord-104269-9r7rqqqk 224 18 Iicr Iicr NNP cord-104269-9r7rqqqk 224 19 LI LI NNP cord-104269-9r7rqqqk 224 20 , , , cord-104269-9r7rqqqk 224 21 LP-*AA LP-*AA NNP cord-104269-9r7rqqqk 225 1 , , , cord-104269-9r7rqqqk 225 2 AA AA NNP cord-104269-9r7rqqqk 225 3 were be VBD cord-104269-9r7rqqqk 225 4 preincubated preincubate VBN cord-104269-9r7rqqqk 225 5 in in IN cord-104269-9r7rqqqk 225 6 serum serum NN cord-104269-9r7rqqqk 225 7 - - HYPH cord-104269-9r7rqqqk 225 8 free free JJ cord-104269-9r7rqqqk 225 9 DME DME NNP cord-104269-9r7rqqqk 225 10 for for IN cord-104269-9r7rqqqk 225 11 30 30 CD cord-104269-9r7rqqqk 225 12 rain rain NN cord-104269-9r7rqqqk 225 13 at at IN cord-104269-9r7rqqqk 225 14 37 37 CD cord-104269-9r7rqqqk 225 15 ° ° NN cord-104269-9r7rqqqk 225 16 C c NN cord-104269-9r7rqqqk 225 17 , , , cord-104269-9r7rqqqk 225 18 and and CC cord-104269-9r7rqqqk 225 19 then then RB cord-104269-9r7rqqqk 225 20 incubated incubate VBD cord-104269-9r7rqqqk 225 21 with with IN cord-104269-9r7rqqqk 225 22 125I 125i CD cord-104269-9r7rqqqk 225 23 - - HYPH cord-104269-9r7rqqqk 225 24 labeled label VBN cord-104269-9r7rqqqk 225 25 Tf Tf NNP cord-104269-9r7rqqqk 225 26 for for IN cord-104269-9r7rqqqk 225 27 1 1 CD cord-104269-9r7rqqqk 225 28 h h NN cord-104269-9r7rqqqk 225 29 at at IN cord-104269-9r7rqqqk 225 30 37 37 CD cord-104269-9r7rqqqk 225 31 ° ° NN cord-104269-9r7rqqqk 225 32 C c NN cord-104269-9r7rqqqk 225 33 . . . cord-104269-9r7rqqqk 226 1 The the DT cord-104269-9r7rqqqk 226 2 cells cell NNS cord-104269-9r7rqqqk 226 3 were be VBD cord-104269-9r7rqqqk 226 4 then then RB cord-104269-9r7rqqqk 226 5 washed wash VBN cord-104269-9r7rqqqk 226 6 and and CC cord-104269-9r7rqqqk 226 7 reincubated reincubate VBD cord-104269-9r7rqqqk 226 8 at at IN cord-104269-9r7rqqqk 226 9 37 37 CD cord-104269-9r7rqqqk 226 10 ° ° NN cord-104269-9r7rqqqk 226 11 C c NN cord-104269-9r7rqqqk 226 12 in in IN cord-104269-9r7rqqqk 226 13 DME DME NNP cord-104269-9r7rqqqk 226 14 containing contain VBG cord-104269-9r7rqqqk 226 15 50 50 CD cord-104269-9r7rqqqk 226 16 # # $ cord-104269-9r7rqqqk 226 17 g g NN cord-104269-9r7rqqqk 226 18 / / SYM cord-104269-9r7rqqqk 226 19 ml ml NN cord-104269-9r7rqqqk 226 20 unlabeled unlabeled JJ cord-104269-9r7rqqqk 226 21 Tf Tf NNP cord-104269-9r7rqqqk 226 22 for for IN cord-104269-9r7rqqqk 226 23 various various JJ cord-104269-9r7rqqqk 226 24 times time NNS cord-104269-9r7rqqqk 226 25 . . . cord-104269-9r7rqqqk 227 1 Acid acid NN cord-104269-9r7rqqqk 227 2 - - HYPH cord-104269-9r7rqqqk 227 3 soluble soluble JJ cord-104269-9r7rqqqk 227 4 radioactivity radioactivity NN cord-104269-9r7rqqqk 227 5 ( ( -LRB- cord-104269-9r7rqqqk 227 6 B b NN cord-104269-9r7rqqqk 227 7 ) ) -RRB- cord-104269-9r7rqqqk 227 8 or or CC cord-104269-9r7rqqqk 227 9 acid acid NN cord-104269-9r7rqqqk 227 10 - - HYPH cord-104269-9r7rqqqk 227 11 insoluble insoluble JJ cord-104269-9r7rqqqk 227 12 125I 125i CD cord-104269-9r7rqqqk 227 13 - - HYPH cord-104269-9r7rqqqk 227 14 labeled label VBN cord-104269-9r7rqqqk 227 15 Tf Tf NNP cord-104269-9r7rqqqk 227 16 ( ( -LRB- cord-104269-9r7rqqqk 227 17 A a NN cord-104269-9r7rqqqk 227 18 ) ) -RRB- cord-104269-9r7rqqqk 227 19 released release VBN cord-104269-9r7rqqqk 227 20 into into IN cord-104269-9r7rqqqk 227 21 the the DT cord-104269-9r7rqqqk 227 22 medium medium NN cord-104269-9r7rqqqk 227 23 , , , cord-104269-9r7rqqqk 227 24 as as RB cord-104269-9r7rqqqk 227 25 well well RB cord-104269-9r7rqqqk 227 26 as as IN cord-104269-9r7rqqqk 227 27 surface surface NN cord-104269-9r7rqqqk 227 28 - - HYPH cord-104269-9r7rqqqk 227 29 bound bind VBN cord-104269-9r7rqqqk 227 30 125I 125I NNP cord-104269-9r7rqqqk 227 31 - - HYPH cord-104269-9r7rqqqk 227 32 labeled label VBN cord-104269-9r7rqqqk 227 33 " " `` cord-104269-9r7rqqqk 227 34 IT it NN cord-104269-9r7rqqqk 227 35 ( ( -LRB- cord-104269-9r7rqqqk 227 36 n n NN cord-104269-9r7rqqqk 227 37 ) ) -RRB- cord-104269-9r7rqqqk 227 38 and and CC cord-104269-9r7rqqqk 227 39 internalized internalize VBD cord-104269-9r7rqqqk 227 40 12SI 12si CD cord-104269-9r7rqqqk 227 41 - - HYPH cord-104269-9r7rqqqk 227 42 labeled label VBN cord-104269-9r7rqqqk 227 43 Tf Tf NNP cord-104269-9r7rqqqk 227 44 ( ( -LRB- cord-104269-9r7rqqqk 227 45 0 0 NFP cord-104269-9r7rqqqk 227 46 ) ) -RRB- cord-104269-9r7rqqqk 227 47 were be VBD cord-104269-9r7rqqqk 227 48 determined determine VBN cord-104269-9r7rqqqk 227 49 as as IN cord-104269-9r7rqqqk 227 50 described describe VBN cord-104269-9r7rqqqk 227 51 in in IN cord-104269-9r7rqqqk 227 52 Materials material NNS cord-104269-9r7rqqqk 227 53 and and CC cord-104269-9r7rqqqk 227 54 Methods Methods NNPS cord-104269-9r7rqqqk 227 55 and and CC cord-104269-9r7rqqqk 227 56 are be VBP cord-104269-9r7rqqqk 227 57 expressed express VBN cord-104269-9r7rqqqk 227 58 as as IN cord-104269-9r7rqqqk 227 59 a a DT cord-104269-9r7rqqqk 227 60 percent percent NN cord-104269-9r7rqqqk 227 61 of of IN cord-104269-9r7rqqqk 227 62 total total JJ cord-104269-9r7rqqqk 227 63 radioactivity radioactivity NN cord-104269-9r7rqqqk 227 64 recovered recover VBN cord-104269-9r7rqqqk 227 65 . . . cord-104269-9r7rqqqk 228 1 Each each DT cord-104269-9r7rqqqk 228 2 point point NN cord-104269-9r7rqqqk 228 3 is be VBZ cord-104269-9r7rqqqk 228 4 the the DT cord-104269-9r7rqqqk 228 5 average average NN cord-104269-9r7rqqqk 228 6 of of IN cord-104269-9r7rqqqk 228 7 triplicate triplicate JJ cord-104269-9r7rqqqk 228 8 determinations determination NNS cord-104269-9r7rqqqk 228 9 from from IN cord-104269-9r7rqqqk 228 10 a a DT cord-104269-9r7rqqqk 228 11 representative representative JJ cord-104269-9r7rqqqk 228 12 experiment experiment NN cord-104269-9r7rqqqk 228 13 . . . cord-104269-9r7rqqqk 229 1 was be VBD cord-104269-9r7rqqqk 229 2 monitored monitor VBN cord-104269-9r7rqqqk 229 3 by by IN cord-104269-9r7rqqqk 229 4 measuring measure VBG cord-104269-9r7rqqqk 229 5 TCA TCA NNP cord-104269-9r7rqqqk 229 6 insoluble insoluble JJ cord-104269-9r7rqqqk 229 7 and and CC cord-104269-9r7rqqqk 229 8 soluble soluble JJ cord-104269-9r7rqqqk 229 9 radioactivity radioactivity NN cord-104269-9r7rqqqk 229 10 . . . cord-104269-9r7rqqqk 230 1 As as IN cord-104269-9r7rqqqk 230 2 expected expect VBN cord-104269-9r7rqqqk 230 3 , , , cord-104269-9r7rqqqk 230 4 the the DT cord-104269-9r7rqqqk 230 5 apo apo NNP cord-104269-9r7rqqqk 230 6 - - HYPH cord-104269-9r7rqqqk 230 7 Tf Tf NNP cord-104269-9r7rqqqk 230 8 released release VBD cord-104269-9r7rqqqk 230 9 into into IN cord-104269-9r7rqqqk 230 10 the the DT cord-104269-9r7rqqqk 230 11 medium medium NN cord-104269-9r7rqqqk 230 12 from from IN cord-104269-9r7rqqqk 230 13 cells cell NNS cord-104269-9r7rqqqk 230 14 expressing express VBG cord-104269-9r7rqqqk 230 15 the the DT cord-104269-9r7rqqqk 230 16 wild wild JJ cord-104269-9r7rqqqk 230 17 - - HYPH cord-104269-9r7rqqqk 230 18 type type NN cord-104269-9r7rqqqk 230 19 TR tr NN cord-104269-9r7rqqqk 230 20 was be VBD cord-104269-9r7rqqqk 230 21 undergraded undergrade VBN cord-104269-9r7rqqqk 230 22 ( ( -LRB- cord-104269-9r7rqqqk 230 23 Fig Fig NNP cord-104269-9r7rqqqk 230 24 . . NNP cord-104269-9r7rqqqk 230 25 7 7 CD cord-104269-9r7rqqqk 230 26 ) ) -RRB- cord-104269-9r7rqqqk 230 27 , , , cord-104269-9r7rqqqk 230 28 as as IN cord-104269-9r7rqqqk 230 29 TR TR NNP cord-104269-9r7rqqqk 230 30 - - HYPH cord-104269-9r7rqqqk 230 31 apo apo NNP cord-104269-9r7rqqqk 230 32 - - HYPH cord-104269-9r7rqqqk 230 33 Tf Tf NNP cord-104269-9r7rqqqk 230 34 complexes complex NNS cord-104269-9r7rqqqk 230 35 are be VBP cord-104269-9r7rqqqk 230 36 efficiently efficiently RB cord-104269-9r7rqqqk 230 37 recycled recycle VBN cord-104269-9r7rqqqk 230 38 back back RB cord-104269-9r7rqqqk 230 39 to to IN cord-104269-9r7rqqqk 230 40 the the DT cord-104269-9r7rqqqk 230 41 cell cell NN cord-104269-9r7rqqqk 230 42 surface surface NN cord-104269-9r7rqqqk 230 43 through through IN cord-104269-9r7rqqqk 230 44 the the DT cord-104269-9r7rqqqk 230 45 sorting sorting NN cord-104269-9r7rqqqk 230 46 and and CC cord-104269-9r7rqqqk 230 47 recycling recycling NN cord-104269-9r7rqqqk 230 48 endosomal endosomal NNP cord-104269-9r7rqqqk 230 49 compartments compartment NNS cord-104269-9r7rqqqk 230 50 Hopkins Hopkins NNP cord-104269-9r7rqqqk 230 51 and and CC cord-104269-9r7rqqqk 230 52 Trowbridge Trowbridge NNP cord-104269-9r7rqqqk 230 53 , , , cord-104269-9r7rqqqk 230 54 1983 1983 CD cord-104269-9r7rqqqk 230 55 ; ; : cord-104269-9r7rqqqk 230 56 Dunn Dunn NNP cord-104269-9r7rqqqk 230 57 et et NNP cord-104269-9r7rqqqk 230 58 al al NNP cord-104269-9r7rqqqk 230 59 . . . cord-104269-9r7rqqqk 231 1 , , , cord-104269-9r7rqqqk 231 2 1989 1989 CD cord-104269-9r7rqqqk 231 3 ; ; : cord-104269-9r7rqqqk 231 4 Weissman Weissman NNP cord-104269-9r7rqqqk 231 5 et et NNP cord-104269-9r7rqqqk 231 6 al al NNP cord-104269-9r7rqqqk 231 7 . . . cord-104269-9r7rqqqk 232 1 , , , cord-104269-9r7rqqqk 232 2 1986 1986 CD cord-104269-9r7rqqqk 232 3 ; ; : cord-104269-9r7rqqqk 232 4 Trowbridge Trowbridge NNP cord-104269-9r7rqqqk 232 5 et et NNP cord-104269-9r7rqqqk 232 6 al al NNP cord-104269-9r7rqqqk 232 7 . . NNP cord-104269-9r7rqqqk 232 8 , , , cord-104269-9r7rqqqk 232 9 1993 1993 CD cord-104269-9r7rqqqk 232 10 ) ) -RRB- cord-104269-9r7rqqqk 232 11 . . . cord-104269-9r7rqqqk 233 1 In in IN cord-104269-9r7rqqqk 233 2 stalking stalk VBG cord-104269-9r7rqqqk 233 3 contrast contrast NN cord-104269-9r7rqqqk 233 4 , , , cord-104269-9r7rqqqk 233 5 , , , cord-104269-9r7rqqqk 233 6 x,35 x,35 NN cord-104269-9r7rqqqk 233 7 % % NN cord-104269-9r7rqqqk 233 8 of of IN cord-104269-9r7rqqqk 233 9 the the DT cord-104269-9r7rqqqk 233 10 l l NN cord-104269-9r7rqqqk 233 11 ~ ~ NFP cord-104269-9r7rqqqk 233 12 I I NNP cord-104269-9r7rqqqk 233 13 - - HYPH cord-104269-9r7rqqqk 233 14 labeled label VBN cord-104269-9r7rqqqk 233 15 Tf Tf NNP cord-104269-9r7rqqqk 233 16 released release VBN cord-104269-9r7rqqqk 233 17 from from IN cord-104269-9r7rqqqk 233 18 cells cell NNS cord-104269-9r7rqqqk 233 19 expressing express VBG cord-104269-9r7rqqqk 233 20 the the DT cord-104269-9r7rqqqk 233 21 Iicr÷m Iicr÷m NNP cord-104269-9r7rqqqk 233 22 chimera chimera NN cord-104269-9r7rqqqk 233 23 was be VBD cord-104269-9r7rqqqk 233 24 degraded degrade VBN cord-104269-9r7rqqqk 233 25 , , , cord-104269-9r7rqqqk 233 26 implying imply VBG cord-104269-9r7rqqqk 233 27 that that IN cord-104269-9r7rqqqk 233 28 this this DT cord-104269-9r7rqqqk 233 29 fraction fraction NN cord-104269-9r7rqqqk 233 30 of of IN cord-104269-9r7rqqqk 233 31 the the DT cord-104269-9r7rqqqk 233 32 chimeric chimeric JJ cord-104269-9r7rqqqk 233 33 receptors receptor NNS cord-104269-9r7rqqqk 233 34 traffic traffic NN cord-104269-9r7rqqqk 233 35 directly directly RB cord-104269-9r7rqqqk 233 36 from from IN cord-104269-9r7rqqqk 233 37 the the DT cord-104269-9r7rqqqk 233 38 cell cell NN cord-104269-9r7rqqqk 233 39 surface surface NN cord-104269-9r7rqqqk 233 40 to to IN cord-104269-9r7rqqqk 233 41 an an DT cord-104269-9r7rqqqk 233 42 endocytic endocytic JJ cord-104269-9r7rqqqk 233 43 compartment compartment NN cord-104269-9r7rqqqk 233 44 where where WRB cord-104269-9r7rqqqk 233 45 they -PRON- PRP cord-104269-9r7rqqqk 233 46 are be VBP cord-104269-9r7rqqqk 233 47 degraded degrade VBN cord-104269-9r7rqqqk 233 48 . . . cord-104269-9r7rqqqk 234 1 A a DT cord-104269-9r7rqqqk 234 2 significantly significantly RB cord-104269-9r7rqqqk 234 3 smaller small JJR cord-104269-9r7rqqqk 234 4 fraction fraction NN cord-104269-9r7rqqqk 234 5 ( ( -LRB- cord-104269-9r7rqqqk 234 6 ~,20 ~,20 CD cord-104269-9r7rqqqk 234 7 % % NN cord-104269-9r7rqqqk 234 8 ) ) -RRB- cord-104269-9r7rqqqk 234 9 of of IN cord-104269-9r7rqqqk 234 10 Tf Tf NNP cord-104269-9r7rqqqk 234 11 bound bind VBN cord-104269-9r7rqqqk 234 12 to to IN cord-104269-9r7rqqqk 234 13 the the DT cord-104269-9r7rqqqk 234 14 Iicr Iicr NNP cord-104269-9r7rqqqk 234 15 chimera chimera NN cord-104269-9r7rqqqk 234 16 was be VBD cord-104269-9r7rqqqk 234 17 degraded degrade VBN cord-104269-9r7rqqqk 234 18 ( ( -LRB- cord-104269-9r7rqqqk 234 19 Fig Fig NNP cord-104269-9r7rqqqk 234 20 . . NNP cord-104269-9r7rqqqk 234 21 7 7 CD cord-104269-9r7rqqqk 234 22 ) ) -RRB- cord-104269-9r7rqqqk 234 23 , , , cord-104269-9r7rqqqk 234 24 indicating indicate VBG cord-104269-9r7rqqqk 234 25 that that IN cord-104269-9r7rqqqk 234 26 the the DT cord-104269-9r7rqqqk 234 27 Ii Ii NNP cord-104269-9r7rqqqk 234 28 transmembrane transmembrane NNP cord-104269-9r7rqqqk 234 29 region region NN cord-104269-9r7rqqqk 234 30 influences influence VBZ cord-104269-9r7rqqqk 234 31 trafficking traffic VBG cord-104269-9r7rqqqk 234 32 from from IN cord-104269-9r7rqqqk 234 33 the the DT cord-104269-9r7rqqqk 234 34 cell cell NN cord-104269-9r7rqqqk 234 35 surface surface NN cord-104269-9r7rqqqk 234 36 to to IN cord-104269-9r7rqqqk 234 37 this this DT cord-104269-9r7rqqqk 234 38 compartment compartment NN cord-104269-9r7rqqqk 234 39 . . . cord-104269-9r7rqqqk 235 1 The the DT cord-104269-9r7rqqqk 235 2 Ii Ii NNP cord-104269-9r7rqqqk 235 3 - - HYPH cord-104269-9r7rqqqk 235 4 TR TR NNP cord-104269-9r7rqqqk 235 5 chimera chimera NN cord-104269-9r7rqqqk 235 6 lacking lack VBG cord-104269-9r7rqqqk 235 7 nontyrosine nontyrosine NN cord-104269-9r7rqqqk 235 8 - - HYPH cord-104269-9r7rqqqk 235 9 based base VBN cord-104269-9r7rqqqk 235 10 sorting sorting NN cord-104269-9r7rqqqk 235 11 signals signal NNS cord-104269-9r7rqqqk 235 12 was be VBD cord-104269-9r7rqqqk 235 13 degraded degrade VBN cord-104269-9r7rqqqk 235 14 only only RB cord-104269-9r7rqqqk 235 15 to to IN cord-104269-9r7rqqqk 235 16 a a DT cord-104269-9r7rqqqk 235 17 slightly slightly RB cord-104269-9r7rqqqk 235 18 higher high JJR cord-104269-9r7rqqqk 235 19 extent extent NN cord-104269-9r7rqqqk 235 20 than than IN cord-104269-9r7rqqqk 235 21 wild wild JJ cord-104269-9r7rqqqk 235 22 - - HYPH cord-104269-9r7rqqqk 235 23 type type NN cord-104269-9r7rqqqk 235 24 TR tr NN cord-104269-9r7rqqqk 235 25 . . . cord-104269-9r7rqqqk 236 1 To to TO cord-104269-9r7rqqqk 236 2 confirm confirm VB cord-104269-9r7rqqqk 236 3 the the DT cord-104269-9r7rqqqk 236 4 inference inference NN cord-104269-9r7rqqqk 236 5 from from IN cord-104269-9r7rqqqk 236 6 these these DT cord-104269-9r7rqqqk 236 7 studies study NNS cord-104269-9r7rqqqk 236 8 that that WDT cord-104269-9r7rqqqk 236 9 the the DT cord-104269-9r7rqqqk 236 10 Ii Ii NNP cord-104269-9r7rqqqk 236 11 - - HYPH cord-104269-9r7rqqqk 236 12 TR TR NNP cord-104269-9r7rqqqk 236 13 chimeras chimera NNS cord-104269-9r7rqqqk 236 14 traffic traffic NN cord-104269-9r7rqqqk 236 15 from from IN cord-104269-9r7rqqqk 236 16 the the DT cord-104269-9r7rqqqk 236 17 plasma plasma NN cord-104269-9r7rqqqk 236 18 membrane membrane NN cord-104269-9r7rqqqk 236 19 to to IN cord-104269-9r7rqqqk 236 20 an an DT cord-104269-9r7rqqqk 236 21 endocytic endocytic JJ cord-104269-9r7rqqqk 236 22 compartment compartment NN cord-104269-9r7rqqqk 236 23 where where WRB cord-104269-9r7rqqqk 236 24 they -PRON- PRP cord-104269-9r7rqqqk 236 25 are be VBP cord-104269-9r7rqqqk 236 26 degraded degrade VBN cord-104269-9r7rqqqk 236 27 , , , cord-104269-9r7rqqqk 236 28 CEF CEF NNP cord-104269-9r7rqqqk 236 29 expressing express VBG cord-104269-9r7rqqqk 236 30 IicT IicT NNP cord-104269-9r7rqqqk 236 31 , , , cord-104269-9r7rqqqk 236 32 Iicr+m Iicr+m NNP cord-104269-9r7rqqqk 236 33 , , , cord-104269-9r7rqqqk 236 34 or or CC cord-104269-9r7rqqqk 236 35 Iicr Iicr NNP cord-104269-9r7rqqqk 236 36 LI LI NNP cord-104269-9r7rqqqk 236 37 , , , cord-104269-9r7rqqqk 236 38 LP-*AA LP-*AA NNP cord-104269-9r7rqqqk 236 39 , , , cord-104269-9r7rqqqk 236 40 AA AA NNP cord-104269-9r7rqqqk 236 41 were be VBD cord-104269-9r7rqqqk 236 42 surface surface NN cord-104269-9r7rqqqk 236 43 - - HYPH cord-104269-9r7rqqqk 236 44 iodinated iodinate VBN cord-104269-9r7rqqqk 236 45 and and CC cord-104269-9r7rqqqk 236 46 the the DT cord-104269-9r7rqqqk 236 47 rate rate NN cord-104269-9r7rqqqk 236 48 of of IN cord-104269-9r7rqqqk 236 49 degradation degradation NN cord-104269-9r7rqqqk 236 50 of of IN cord-104269-9r7rqqqk 236 51 receptors receptor NNS cord-104269-9r7rqqqk 236 52 transiently transiently RB cord-104269-9r7rqqqk 236 53 located locate VBN cord-104269-9r7rqqqk 236 54 at at IN cord-104269-9r7rqqqk 236 55 the the DT cord-104269-9r7rqqqk 236 56 plasma plasma NN cord-104269-9r7rqqqk 236 57 membrane membrane NN cord-104269-9r7rqqqk 236 58 directly directly RB cord-104269-9r7rqqqk 236 59 determined determine VBN cord-104269-9r7rqqqk 236 60 . . . cord-104269-9r7rqqqk 237 1 As as IN cord-104269-9r7rqqqk 237 2 shown show VBN cord-104269-9r7rqqqk 237 3 in in IN cord-104269-9r7rqqqk 237 4 Fig Fig NNP cord-104269-9r7rqqqk 237 5 . . . cord-104269-9r7rqqqk 238 1 8 8 CD cord-104269-9r7rqqqk 238 2 , , , cord-104269-9r7rqqqk 238 3 surface surface NN cord-104269-9r7rqqqk 238 4 - - HYPH cord-104269-9r7rqqqk 238 5 iodinated iodinate VBN cord-104269-9r7rqqqk 238 6 Iicr Iicr NNP cord-104269-9r7rqqqk 238 7 chimera chimera NN cord-104269-9r7rqqqk 238 8 ( ( -LRB- cord-104269-9r7rqqqk 238 9 half half JJ cord-104269-9r7rqqqk 238 10 - - HYPH cord-104269-9r7rqqqk 238 11 life life NN cord-104269-9r7rqqqk 238 12 •6 •6 . cord-104269-9r7rqqqk 239 1 h h LS cord-104269-9r7rqqqk 239 2 ) ) -RRB- cord-104269-9r7rqqqk 239 3 was be VBD cord-104269-9r7rqqqk 239 4 degraded degrade VBN cord-104269-9r7rqqqk 239 5 more more RBR cord-104269-9r7rqqqk 239 6 rapidly rapidly RB cord-104269-9r7rqqqk 239 7 than than IN cord-104269-9r7rqqqk 239 8 the the DT cord-104269-9r7rqqqk 239 9 chimera chimera NN cord-104269-9r7rqqqk 239 10 lacking lack VBG cord-104269-9r7rqqqk 239 11 non non JJ cord-104269-9r7rqqqk 239 12 - - JJ cord-104269-9r7rqqqk 239 13 tyrosine tyrosine NN cord-104269-9r7rqqqk 239 14 - - HYPH cord-104269-9r7rqqqk 239 15 based base VBN cord-104269-9r7rqqqk 239 16 signals signal NNS cord-104269-9r7rqqqk 239 17 . . . cord-104269-9r7rqqqk 240 1 Surface surface NN cord-104269-9r7rqqqk 240 2 - - HYPH cord-104269-9r7rqqqk 240 3 iodlnated iodlnate VBN cord-104269-9r7rqqqk 240 4 Iicr+~ Iicr+~ NNP cord-104269-9r7rqqqk 240 5 was be VBD cord-104269-9r7rqqqk 240 6 degraded degrade VBN cord-104269-9r7rqqqk 240 7 at at IN cord-104269-9r7rqqqk 240 8 an an DT cord-104269-9r7rqqqk 240 9 even even RB cord-104269-9r7rqqqk 240 10 faster fast JJR cord-104269-9r7rqqqk 240 11 rate rate NN cord-104269-9r7rqqqk 240 12 ( ( -LRB- cord-104269-9r7rqqqk 240 13 half half JJ cord-104269-9r7rqqqk 240 14 - - HYPH cord-104269-9r7rqqqk 240 15 life life NN cord-104269-9r7rqqqk 240 16 ~2 ~2 NNP cord-104269-9r7rqqqk 240 17 - - SYM cord-104269-9r7rqqqk 240 18 3 3 CD cord-104269-9r7rqqqk 240 19 h h NN cord-104269-9r7rqqqk 240 20 ) ) -RRB- cord-104269-9r7rqqqk 240 21 . . . cord-104269-9r7rqqqk 241 1 The the DT cord-104269-9r7rqqqk 241 2 Iicr+~ iicr+~ CD cord-104269-9r7rqqqk 241 3 chimera chimera NN cord-104269-9r7rqqqk 241 4 was be VBD cord-104269-9r7rqqqk 241 5 degraded degrade VBN cord-104269-9r7rqqqk 241 6 more more RBR cord-104269-9r7rqqqk 241 7 rapidly rapidly RB cord-104269-9r7rqqqk 241 8 than than IN cord-104269-9r7rqqqk 241 9 the the DT cord-104269-9r7rqqqk 241 10 Iicv Iicv NNP cord-104269-9r7rqqqk 241 11 chimera chimera NN cord-104269-9r7rqqqk 241 12 , , , cord-104269-9r7rqqqk 241 13 whether whether IN cord-104269-9r7rqqqk 241 14 the the DT cord-104269-9r7rqqqk 241 15 chimeras chimera NNS cord-104269-9r7rqqqk 241 16 were be VBD cord-104269-9r7rqqqk 241 17 delivered deliver VBN cord-104269-9r7rqqqk 241 18 directly directly RB cord-104269-9r7rqqqk 241 19 from from IN cord-104269-9r7rqqqk 241 20 the the DT cord-104269-9r7rqqqk 241 21 trans trans NNP cord-104269-9r7rqqqk 241 22 - - NNP cord-104269-9r7rqqqk 241 23 Golgi Golgi NNP cord-104269-9r7rqqqk 241 24 to to IN cord-104269-9r7rqqqk 241 25 the the DT cord-104269-9r7rqqqk 241 26 endocytic endocytic JJ cord-104269-9r7rqqqk 241 27 pathway pathway NN cord-104269-9r7rqqqk 241 28 or or CC cord-104269-9r7rqqqk 241 29 via via IN cord-104269-9r7rqqqk 241 30 the the DT cord-104269-9r7rqqqk 241 31 cell cell NN cord-104269-9r7rqqqk 241 32 surface surface NN cord-104269-9r7rqqqk 241 33 , , , cord-104269-9r7rqqqk 241 34 implying imply VBG cord-104269-9r7rqqqk 241 35 that that IN cord-104269-9r7rqqqk 241 36 the the DT cord-104269-9r7rqqqk 241 37 Ii Ii NNP cord-104269-9r7rqqqk 241 38 transmembrane transmembrane NNP cord-104269-9r7rqqqk 241 39 region region NN cord-104269-9r7rqqqk 241 40 was be VBD cord-104269-9r7rqqqk 241 41 influencing influence VBG cord-104269-9r7rqqqk 241 42 trafficking trafficking NN cord-104269-9r7rqqqk 241 43 along along IN cord-104269-9r7rqqqk 241 44 both both DT cord-104269-9r7rqqqk 241 45 pathways pathway NNS cord-104269-9r7rqqqk 241 46 . . . cord-104269-9r7rqqqk 242 1 To to TO cord-104269-9r7rqqqk 242 2 determine determine VB cord-104269-9r7rqqqk 242 3 whether whether IN cord-104269-9r7rqqqk 242 4 the the DT cord-104269-9r7rqqqk 242 5 Ii Ii NNP cord-104269-9r7rqqqk 242 6 transmembrane transmembrane NNP cord-104269-9r7rqqqk 242 7 region region NN cord-104269-9r7rqqqk 242 8 was be VBD cord-104269-9r7rqqqk 242 9 sufficient sufficient JJ cord-104269-9r7rqqqk 242 10 to to TO cord-104269-9r7rqqqk 242 11 promote promote VB cord-104269-9r7rqqqk 242 12 delivery delivery NN cord-104269-9r7rqqqk 242 13 of of IN cord-104269-9r7rqqqk 242 14 TR tr NN cord-104269-9r7rqqqk 242 15 to to IN cord-104269-9r7rqqqk 242 16 the the DT cord-104269-9r7rqqqk 242 17 degradative degradative NNP cord-104269-9r7rqqqk 242 18 endocytic endocytic NNP cord-104269-9r7rqqqk 242 19 compartment compartment NN cord-104269-9r7rqqqk 242 20 , , , cord-104269-9r7rqqqk 242 21 a a DT cord-104269-9r7rqqqk 242 22 chimera chimera NN cord-104269-9r7rqqqk 242 23 consisting consist VBG cord-104269-9r7rqqqk 242 24 of of IN cord-104269-9r7rqqqk 242 25 the the DT cord-104269-9r7rqqqk 242 26 TR tr NN cord-104269-9r7rqqqk 242 27 external external JJ cord-104269-9r7rqqqk 242 28 domain domain NN cord-104269-9r7rqqqk 242 29 and and CC cord-104269-9r7rqqqk 242 30 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 242 31 tail tail NN cord-104269-9r7rqqqk 242 32 and and CC cord-104269-9r7rqqqk 242 33 the the DT cord-104269-9r7rqqqk 242 34 Ii Ii NNP cord-104269-9r7rqqqk 242 35 transmembrane transmembrane NNP cord-104269-9r7rqqqk 242 36 region region NN cord-104269-9r7rqqqk 242 37 ( ( -LRB- cord-104269-9r7rqqqk 242 38 Iim Iim NNP cord-104269-9r7rqqqk 242 39 ) ) -RRB- cord-104269-9r7rqqqk 242 40 was be VBD cord-104269-9r7rqqqk 242 41 constructed construct VBN cord-104269-9r7rqqqk 242 42 . . . cord-104269-9r7rqqqk 243 1 The the DT cord-104269-9r7rqqqk 243 2 Ii~ Ii~ NNP cord-104269-9r7rqqqk 243 3 chimera chimera NN cord-104269-9r7rqqqk 243 4 was be VBD cord-104269-9r7rqqqk 243 5 expressed express VBN cord-104269-9r7rqqqk 243 6 on on IN cord-104269-9r7rqqqk 243 7 the the DT cord-104269-9r7rqqqk 243 8 cell cell NN cord-104269-9r7rqqqk 243 9 surface surface NN cord-104269-9r7rqqqk 243 10 at at IN cord-104269-9r7rqqqk 243 11 a a DT cord-104269-9r7rqqqk 243 12 level level NN cord-104269-9r7rqqqk 243 13 of of IN cord-104269-9r7rqqqk 243 14 23 23 CD cord-104269-9r7rqqqk 243 15 + + SYM cord-104269-9r7rqqqk 243 16 6 6 CD cord-104269-9r7rqqqk 243 17 % % NN cord-104269-9r7rqqqk 243 18 relative relative JJ cord-104269-9r7rqqqk 243 19 to to IN cord-104269-9r7rqqqk 243 20 the the DT cord-104269-9r7rqqqk 243 21 wild wild JJ cord-104269-9r7rqqqk 243 22 - - HYPH cord-104269-9r7rqqqk 243 23 type type NN cord-104269-9r7rqqqk 243 24 TR tr NN cord-104269-9r7rqqqk 243 25 and and CC cord-104269-9r7rqqqk 243 26 was be VBD cord-104269-9r7rqqqk 243 27 internalized internalize VBN cord-104269-9r7rqqqk 243 28 with with IN cord-104269-9r7rqqqk 243 29 a a DT cord-104269-9r7rqqqk 243 30 relative relative JJ cord-104269-9r7rqqqk 243 31 efficiency efficiency NN cord-104269-9r7rqqqk 243 32 of of IN cord-104269-9r7rqqqk 243 33 76.0 76.0 CD cord-104269-9r7rqqqk 243 34 + + CC cord-104269-9r7rqqqk 243 35 Figure figure NN cord-104269-9r7rqqqk 243 36 8 8 CD cord-104269-9r7rqqqk 243 37 . . . cord-104269-9r7rqqqk 244 1 Degradation degradation NN cord-104269-9r7rqqqk 244 2 of of IN cord-104269-9r7rqqqk 244 3 125I 125i CD cord-104269-9r7rqqqk 244 4 surface surface NN cord-104269-9r7rqqqk 244 5 - - HYPH cord-104269-9r7rqqqk 244 6 labeled label VBN cord-104269-9r7rqqqk 244 7 Ii Ii NNP cord-104269-9r7rqqqk 244 8 - - HYPH cord-104269-9r7rqqqk 244 9 TR TR NNP cord-104269-9r7rqqqk 244 10 chimeras chimera NNS cord-104269-9r7rqqqk 244 11 . . . cord-104269-9r7rqqqk 245 1 Equivalent equivalent JJ cord-104269-9r7rqqqk 245 2 cell cell NN cord-104269-9r7rqqqk 245 3 numbers number NNS cord-104269-9r7rqqqk 245 4 of of IN cord-104269-9r7rqqqk 245 5 CEF CEF NNP cord-104269-9r7rqqqk 245 6 expressing express VBG cord-104269-9r7rqqqk 245 7 either either CC cord-104269-9r7rqqqk 245 8 Iicr Iicr NNP cord-104269-9r7rqqqk 245 9 , , , cord-104269-9r7rqqqk 245 10 IicT+I"M iict+i"m NN cord-104269-9r7rqqqk 245 11 or or CC cord-104269-9r7rqqqk 245 12 Iic~ iic~ NN cord-104269-9r7rqqqk 245 13 LI LI NNP cord-104269-9r7rqqqk 245 14 , , , cord-104269-9r7rqqqk 245 15 LP-*AA LP-*AA NNP cord-104269-9r7rqqqk 245 16 , , , cord-104269-9r7rqqqk 245 17 AA AA NNP cord-104269-9r7rqqqk 245 18 chimeras chimera NNS cord-104269-9r7rqqqk 245 19 were be VBD cord-104269-9r7rqqqk 245 20 surface surface NN cord-104269-9r7rqqqk 245 21 - - HYPH cord-104269-9r7rqqqk 245 22 iodinated iodinate VBN cord-104269-9r7rqqqk 245 23 , , , cord-104269-9r7rqqqk 245 24 and and CC cord-104269-9r7rqqqk 245 25 then then RB cord-104269-9r7rqqqk 245 26 incubated incubate VBN cord-104269-9r7rqqqk 245 27 for for IN cord-104269-9r7rqqqk 245 28 the the DT cord-104269-9r7rqqqk 245 29 times time NNS cord-104269-9r7rqqqk 245 30 indicated indicate VBD cord-104269-9r7rqqqk 245 31 . . . cord-104269-9r7rqqqk 246 1 The the DT cord-104269-9r7rqqqk 246 2 Ii Ii NNP cord-104269-9r7rqqqk 246 3 - - HYPH cord-104269-9r7rqqqk 246 4 TR TR NNP cord-104269-9r7rqqqk 246 5 chimeras chimera NNS cord-104269-9r7rqqqk 246 6 were be VBD cord-104269-9r7rqqqk 246 7 immunoprecipitated immunoprecipitate VBN cord-104269-9r7rqqqk 246 8 from from IN cord-104269-9r7rqqqk 246 9 post post JJ cord-104269-9r7rqqqk 246 10 - - JJ cord-104269-9r7rqqqk 246 11 nuclear nuclear JJ cord-104269-9r7rqqqk 246 12 snpernatants snpernatant NNS cord-104269-9r7rqqqk 246 13 and and CC cord-104269-9r7rqqqk 246 14 analyzed analyze VBD cord-104269-9r7rqqqk 246 15 on on IN cord-104269-9r7rqqqk 246 16 SDS SDS NNP cord-104269-9r7rqqqk 246 17 - - HYPH cord-104269-9r7rqqqk 246 18 polyacrylamide polyacrylamide NN cord-104269-9r7rqqqk 246 19 gels gel NNS cord-104269-9r7rqqqk 246 20 as as IN cord-104269-9r7rqqqk 246 21 described describe VBN cord-104269-9r7rqqqk 246 22 in in IN cord-104269-9r7rqqqk 246 23 Materials material NNS cord-104269-9r7rqqqk 246 24 and and CC cord-104269-9r7rqqqk 246 25 Methods method NNS cord-104269-9r7rqqqk 246 26 . . . cord-104269-9r7rqqqk 247 1 Dried dry VBN cord-104269-9r7rqqqk 247 2 gels gel NNS cord-104269-9r7rqqqk 247 3 were be VBD cord-104269-9r7rqqqk 247 4 exposed expose VBN cord-104269-9r7rqqqk 247 5 to to IN cord-104269-9r7rqqqk 247 6 preflashed preflashe VBN cord-104269-9r7rqqqk 247 7 XAR XAR NNP cord-104269-9r7rqqqk 247 8 film film NN cord-104269-9r7rqqqk 247 9 ( ( -LRB- cord-104269-9r7rqqqk 247 10 Eastman Eastman NNP cord-104269-9r7rqqqk 247 11 Kodak Kodak NNP cord-104269-9r7rqqqk 247 12 , , , cord-104269-9r7rqqqk 247 13 Rochester Rochester NNP cord-104269-9r7rqqqk 247 14 , , , cord-104269-9r7rqqqk 247 15 NY NY NNP cord-104269-9r7rqqqk 247 16 ) ) -RRB- cord-104269-9r7rqqqk 247 17 for for IN cord-104269-9r7rqqqk 247 18 one one CD cord-104269-9r7rqqqk 247 19 day day NN cord-104269-9r7rqqqk 247 20 ( ( -LRB- cord-104269-9r7rqqqk 247 21 licr licr NNP cord-104269-9r7rqqqk 247 22 L1,LP-~A4,A4 L1,LP-~A4,A4 NNP cord-104269-9r7rqqqk 247 23 and and CC cord-104269-9r7rqqqk 247 24 licr licr NNP cord-104269-9r7rqqqk 247 25 ) ) -RRB- cord-104269-9r7rqqqk 247 26 or or CC cord-104269-9r7rqqqk 247 27 2 2 CD cord-104269-9r7rqqqk 247 28 d d NN cord-104269-9r7rqqqk 247 29 ( ( -LRB- cord-104269-9r7rqqqk 247 30 licr+rM licr+rM NNP cord-104269-9r7rqqqk 247 31 ) ) -RRB- cord-104269-9r7rqqqk 247 32 . . . cord-104269-9r7rqqqk 248 1 Figure figure NN cord-104269-9r7rqqqk 248 2 9 9 CD cord-104269-9r7rqqqk 248 3 . . . cord-104269-9r7rqqqk 249 1 li li NNP cord-104269-9r7rqqqk 249 2 transmembrane transmembrane NNP cord-104269-9r7rqqqk 249 3 region region NN cord-104269-9r7rqqqk 249 4 targets target VBZ cord-104269-9r7rqqqk 249 5 the the DT cord-104269-9r7rqqqk 249 6 TR tr NN cord-104269-9r7rqqqk 249 7 to to IN cord-104269-9r7rqqqk 249 8 a a DT cord-104269-9r7rqqqk 249 9 post post JJ cord-104269-9r7rqqqk 249 10 - - JJ cord-104269-9r7rqqqk 249 11 Golgi Golgi NNP cord-104269-9r7rqqqk 249 12 degradative degradative JJ cord-104269-9r7rqqqk 249 13 compartment compartment NN cord-104269-9r7rqqqk 249 14 . . . cord-104269-9r7rqqqk 250 1 Equivalent equivalent JJ cord-104269-9r7rqqqk 250 2 cell cell NN cord-104269-9r7rqqqk 250 3 numbers number NNS cord-104269-9r7rqqqk 250 4 of of IN cord-104269-9r7rqqqk 250 5 CEF CEF NNP cord-104269-9r7rqqqk 250 6 expressing express VBG cord-104269-9r7rqqqk 250 7 either either CC cord-104269-9r7rqqqk 250 8 wild wild JJ cord-104269-9r7rqqqk 250 9 - - HYPH cord-104269-9r7rqqqk 250 10 type type NN cord-104269-9r7rqqqk 250 11 TR tr NN cord-104269-9r7rqqqk 250 12 or or CC cord-104269-9r7rqqqk 250 13 the the DT cord-104269-9r7rqqqk 250 14 IiTM IiTM NNP cord-104269-9r7rqqqk 250 15 chimeric chimeric JJ cord-104269-9r7rqqqk 250 16 receptor receptor NN cord-104269-9r7rqqqk 250 17 were be VBD cord-104269-9r7rqqqk 250 18 pulse pulse NN cord-104269-9r7rqqqk 250 19 - - HYPH cord-104269-9r7rqqqk 250 20 labeled label VBN cord-104269-9r7rqqqk 250 21 with with IN cord-104269-9r7rqqqk 250 22 trans trans NNP cord-104269-9r7rqqqk 250 23 - - JJ cord-104269-9r7rqqqk 250 24 aSS ass NN cord-104269-9r7rqqqk 250 25 - - HYPH cord-104269-9r7rqqqk 250 26 label label NN cord-104269-9r7rqqqk 250 27 and and CC cord-104269-9r7rqqqk 250 28 chased chase VBD cord-104269-9r7rqqqk 250 29 as as IN cord-104269-9r7rqqqk 250 30 described describe VBN cord-104269-9r7rqqqk 250 31 in in IN cord-104269-9r7rqqqk 250 32 the the DT cord-104269-9r7rqqqk 250 33 legend legend NN cord-104269-9r7rqqqk 250 34 for for IN cord-104269-9r7rqqqk 250 35 Fig Fig NNP cord-104269-9r7rqqqk 250 36 . . . cord-104269-9r7rqqqk 251 1 2 2 LS cord-104269-9r7rqqqk 251 2 . . . cord-104269-9r7rqqqk 252 1 TRs tr NNS cord-104269-9r7rqqqk 252 2 were be VBD cord-104269-9r7rqqqk 252 3 then then RB cord-104269-9r7rqqqk 252 4 immunoprecipitated immunoprecipitate VBN cord-104269-9r7rqqqk 252 5 from from IN cord-104269-9r7rqqqk 252 6 postnuclear postnuclear NNP cord-104269-9r7rqqqk 252 7 supernatants supernatants NNP cord-104269-9r7rqqqk 252 8 and and CC cord-104269-9r7rqqqk 252 9 analyzed analyze VBD cord-104269-9r7rqqqk 252 10 on on IN cord-104269-9r7rqqqk 252 11 SDS SDS NNP cord-104269-9r7rqqqk 252 12 - - HYPH cord-104269-9r7rqqqk 252 13 polyacrylamide polyacrylamide NN cord-104269-9r7rqqqk 252 14 gels gel NNS cord-104269-9r7rqqqk 252 15 as as IN cord-104269-9r7rqqqk 252 16 described describe VBN cord-104269-9r7rqqqk 252 17 in in IN cord-104269-9r7rqqqk 252 18 Materials material NNS cord-104269-9r7rqqqk 252 19 and and CC cord-104269-9r7rqqqk 252 20 Methods method NNS cord-104269-9r7rqqqk 252 21 . . . cord-104269-9r7rqqqk 253 1 Dried dry VBN cord-104269-9r7rqqqk 253 2 gels gel NNS cord-104269-9r7rqqqk 253 3 were be VBD cord-104269-9r7rqqqk 253 4 exposed expose VBN cord-104269-9r7rqqqk 253 5 to to IN cord-104269-9r7rqqqk 253 6 preflashed preflashe VBN cord-104269-9r7rqqqk 253 7 XAR XAR NNP cord-104269-9r7rqqqk 253 8 film film NN cord-104269-9r7rqqqk 253 9 for for IN cord-104269-9r7rqqqk 253 10 two two CD cord-104269-9r7rqqqk 253 11 days day NNS cord-104269-9r7rqqqk 254 1 ( ( -LRB- cord-104269-9r7rqqqk 254 2 Eastman Eastman NNP cord-104269-9r7rqqqk 254 3 Kodak Kodak NNP cord-104269-9r7rqqqk 254 4 , , , cord-104269-9r7rqqqk 254 5 Rochester Rochester NNP cord-104269-9r7rqqqk 254 6 , , , cord-104269-9r7rqqqk 254 7 NY NY NNP cord-104269-9r7rqqqk 254 8 ) ) -RRB- cord-104269-9r7rqqqk 254 9 , , , cord-104269-9r7rqqqk 254 10 and and CC cord-104269-9r7rqqqk 254 11 immtmoprecipitates immtmoprecipitates NNPS cord-104269-9r7rqqqk 254 12 were be VBD cord-104269-9r7rqqqk 254 13 quantitated quantitate VBN cord-104269-9r7rqqqk 254 14 on on IN cord-104269-9r7rqqqk 254 15 a a DT cord-104269-9r7rqqqk 254 16 model model NN cord-104269-9r7rqqqk 254 17 425 425 CD cord-104269-9r7rqqqk 254 18 Phosphorlmager Phosphorlmager NNP cord-104269-9r7rqqqk 254 19 ( ( -LRB- cord-104269-9r7rqqqk 254 20 Molecular Molecular NNP cord-104269-9r7rqqqk 254 21 Dynamics Dynamics NNPS cord-104269-9r7rqqqk 254 22 ) ) -RRB- cord-104269-9r7rqqqk 254 23 . . . cord-104269-9r7rqqqk 255 1 0.5 0.5 CD cord-104269-9r7rqqqk 255 2 % % NN cord-104269-9r7rqqqk 255 3 . . . cord-104269-9r7rqqqk 256 1 Importantly importantly RB cord-104269-9r7rqqqk 256 2 , , , cord-104269-9r7rqqqk 256 3 pulse pulse NN cord-104269-9r7rqqqk 256 4 - - HYPH cord-104269-9r7rqqqk 256 5 chase chase NN cord-104269-9r7rqqqk 256 6 analysis analysis NN cord-104269-9r7rqqqk 256 7 showed show VBD cord-104269-9r7rqqqk 256 8 that that IN cord-104269-9r7rqqqk 256 9 the the DT cord-104269-9r7rqqqk 256 10 fully fully RB cord-104269-9r7rqqqk 256 11 glycosylated glycosylate VBN cord-104269-9r7rqqqk 256 12 IiTM IiTM NNP cord-104269-9r7rqqqk 256 13 chimera chimera NN cord-104269-9r7rqqqk 256 14 was be VBD cord-104269-9r7rqqqk 256 15 rapidly rapidly RB cord-104269-9r7rqqqk 256 16 degraded degrade VBN cord-104269-9r7rqqqk 256 17 ( ( -LRB- cord-104269-9r7rqqqk 256 18 Fig fig NN cord-104269-9r7rqqqk 256 19 . . . cord-104269-9r7rqqqk 256 20 _SP cord-104269-9r7rqqqk 256 21 9 9 CD cord-104269-9r7rqqqk 256 22 ) ) -RRB- cord-104269-9r7rqqqk 256 23 with with IN cord-104269-9r7rqqqk 256 24 a a DT cord-104269-9r7rqqqk 256 25 half half JJ cord-104269-9r7rqqqk 256 26 - - HYPH cord-104269-9r7rqqqk 256 27 life life NN cord-104269-9r7rqqqk 256 28 of of IN cord-104269-9r7rqqqk 256 29 , , , cord-104269-9r7rqqqk 256 30 , , , cord-104269-9r7rqqqk 256 31 06 06 CD cord-104269-9r7rqqqk 256 32 h h NNP cord-104269-9r7rqqqk 256 33 , , , cord-104269-9r7rqqqk 256 34 similar similar JJ cord-104269-9r7rqqqk 256 35 to to IN cord-104269-9r7rqqqk 256 36 that that DT cord-104269-9r7rqqqk 256 37 of of IN cord-104269-9r7rqqqk 256 38 the the DT cord-104269-9r7rqqqk 256 39 Iicr Iicr NNP cord-104269-9r7rqqqk 256 40 chimera chimera NN cord-104269-9r7rqqqk 256 41 . . . cord-104269-9r7rqqqk 257 1 By by IN cord-104269-9r7rqqqk 257 2 constructing construct VBG cord-104269-9r7rqqqk 257 3 11-TR 11-tr CD cord-104269-9r7rqqqk 257 4 chimeras chimera NNS cord-104269-9r7rqqqk 257 5 comprised comprise VBN cord-104269-9r7rqqqk 257 6 of of IN cord-104269-9r7rqqqk 257 7 the the DT cord-104269-9r7rqqqk 257 8 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 257 9 domain domain NN cord-104269-9r7rqqqk 257 10 of of IN cord-104269-9r7rqqqk 257 11 Ii Ii NNP cord-104269-9r7rqqqk 257 12 , , , cord-104269-9r7rqqqk 257 13 the the DT cord-104269-9r7rqqqk 257 14 external external JJ cord-104269-9r7rqqqk 257 15 domain domain NN cord-104269-9r7rqqqk 257 16 of of IN cord-104269-9r7rqqqk 257 17 the the DT cord-104269-9r7rqqqk 257 18 human human JJ cord-104269-9r7rqqqk 257 19 TR tr NN cord-104269-9r7rqqqk 257 20 , , , cord-104269-9r7rqqqk 257 21 and and CC cord-104269-9r7rqqqk 257 22 the the DT cord-104269-9r7rqqqk 257 23 transmembrane transmembrane NN cord-104269-9r7rqqqk 257 24 region region NN cord-104269-9r7rqqqk 257 25 of of IN cord-104269-9r7rqqqk 257 26 either either DT cord-104269-9r7rqqqk 257 27 Ii Ii NNP cord-104269-9r7rqqqk 257 28 or or CC cord-104269-9r7rqqqk 257 29 TR TR NNP cord-104269-9r7rqqqk 257 30 , , , cord-104269-9r7rqqqk 257 31 we -PRON- PRP cord-104269-9r7rqqqk 257 32 have have VBP cord-104269-9r7rqqqk 257 33 been be VBN cord-104269-9r7rqqqk 257 34 able able JJ cord-104269-9r7rqqqk 257 35 to to TO cord-104269-9r7rqqqk 257 36 take take VB cord-104269-9r7rqqqk 257 37 advantage advantage NN cord-104269-9r7rqqqk 257 38 of of IN cord-104269-9r7rqqqk 257 39 the the DT cord-104269-9r7rqqqk 257 40 quantitative quantitative JJ cord-104269-9r7rqqqk 257 41 assays assay NNS cord-104269-9r7rqqqk 257 42 available available JJ cord-104269-9r7rqqqk 257 43 for for IN cord-104269-9r7rqqqk 257 44 measuring measure VBG cord-104269-9r7rqqqk 257 45 the the DT cord-104269-9r7rqqqk 257 46 rate rate NN cord-104269-9r7rqqqk 257 47 of of IN cord-104269-9r7rqqqk 257 48 internalization internalization NN cord-104269-9r7rqqqk 257 49 of of IN cord-104269-9r7rqqqk 257 50 TRs tr NNS cord-104269-9r7rqqqk 257 51 from from IN cord-104269-9r7rqqqk 257 52 the the DT cord-104269-9r7rqqqk 257 53 cell cell NN cord-104269-9r7rqqqk 257 54 surface surface NN cord-104269-9r7rqqqk 257 55 . . . cord-104269-9r7rqqqk 258 1 This this DT cord-104269-9r7rqqqk 258 2 experimental experimental JJ cord-104269-9r7rqqqk 258 3 strategy strategy NN cord-104269-9r7rqqqk 258 4 has have VBZ cord-104269-9r7rqqqk 258 5 allowed allow VBN cord-104269-9r7rqqqk 258 6 us -PRON- PRP cord-104269-9r7rqqqk 258 7 to to TO cord-104269-9r7rqqqk 258 8 demonstrate demonstrate VB cord-104269-9r7rqqqk 258 9 that that IN cord-104269-9r7rqqqk 258 10 the the DT cord-104269-9r7rqqqk 258 11 Ii Ii NNP cord-104269-9r7rqqqk 258 12 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 258 13 tall tall JJ cord-104269-9r7rqqqk 258 14 can can MD cord-104269-9r7rqqqk 258 15 promote promote VB cord-104269-9r7rqqqk 258 16 rapid rapid JJ cord-104269-9r7rqqqk 258 17 internalization internalization NN cord-104269-9r7rqqqk 258 18 of of IN cord-104269-9r7rqqqk 258 19 TR tr NN cord-104269-9r7rqqqk 258 20 and and CC cord-104269-9r7rqqqk 258 21 to to TO cord-104269-9r7rqqqk 258 22 identify identify VB cord-104269-9r7rqqqk 258 23 residues residue NNS cord-104269-9r7rqqqk 258 24 within within IN cord-104269-9r7rqqqk 258 25 the the DT cord-104269-9r7rqqqk 258 26 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 258 27 tall tall NN cord-104269-9r7rqqqk 258 28 of of IN cord-104269-9r7rqqqk 258 29 1i 1i CD cord-104269-9r7rqqqk 258 30 that that WDT cord-104269-9r7rqqqk 258 31 are be VBP cord-104269-9r7rqqqk 258 32 required require VBN cord-104269-9r7rqqqk 258 33 for for IN cord-104269-9r7rqqqk 258 34 this this DT cord-104269-9r7rqqqk 258 35 activity activity NN cord-104269-9r7rqqqk 258 36 . . . cord-104269-9r7rqqqk 259 1 Our -PRON- PRP$ cord-104269-9r7rqqqk 259 2 data datum NNS cord-104269-9r7rqqqk 259 3 indicate indicate VBP cord-104269-9r7rqqqk 259 4 that that IN cord-104269-9r7rqqqk 259 5 the the DT cord-104269-9r7rqqqk 259 6 11 11 CD cord-104269-9r7rqqqk 259 7 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 259 8 tail tail NN cord-104269-9r7rqqqk 259 9 contains contain VBZ cord-104269-9r7rqqqk 259 10 two two CD cord-104269-9r7rqqqk 259 11 independent independent JJ cord-104269-9r7rqqqk 259 12 non non JJ cord-104269-9r7rqqqk 259 13 - - JJ cord-104269-9r7rqqqk 259 14 tyrosine tyrosine NN cord-104269-9r7rqqqk 259 15 - - HYPH cord-104269-9r7rqqqk 259 16 based base VBN cord-104269-9r7rqqqk 259 17 sorting sorting NN cord-104269-9r7rqqqk 259 18 signals signal NNS cord-104269-9r7rqqqk 259 19 that that WDT cord-104269-9r7rqqqk 259 20 promote promote VBP cord-104269-9r7rqqqk 259 21 rapid rapid JJ cord-104269-9r7rqqqk 259 22 internalization internalization NN cord-104269-9r7rqqqk 259 23 . . . cord-104269-9r7rqqqk 260 1 The the DT cord-104269-9r7rqqqk 260 2 same same JJ cord-104269-9r7rqqqk 260 3 or or CC cord-104269-9r7rqqqk 260 4 overlapping overlapping JJ cord-104269-9r7rqqqk 260 5 signals signal NNS cord-104269-9r7rqqqk 260 6 are be VBP cord-104269-9r7rqqqk 260 7 recognized recognize VBN cord-104269-9r7rqqqk 260 8 in in IN cord-104269-9r7rqqqk 260 9 the the DT cord-104269-9r7rqqqk 260 10 trans trans NNP cord-104269-9r7rqqqk 260 11 - - NNP cord-104269-9r7rqqqk 260 12 Golgi Golgi NNP cord-104269-9r7rqqqk 260 13 and and CC cord-104269-9r7rqqqk 260 14 mediate mediate VB cord-104269-9r7rqqqk 260 15 direct direct JJ cord-104269-9r7rqqqk 260 16 sorting sorting NN cord-104269-9r7rqqqk 260 17 along along IN cord-104269-9r7rqqqk 260 18 an an DT cord-104269-9r7rqqqk 260 19 intracellular intracellular JJ cord-104269-9r7rqqqk 260 20 route route NN cord-104269-9r7rqqqk 260 21 to to IN cord-104269-9r7rqqqk 260 22 the the DT cord-104269-9r7rqqqk 260 23 endocytic endocytic JJ cord-104269-9r7rqqqk 260 24 pathway pathway NN cord-104269-9r7rqqqk 260 25 . . . cord-104269-9r7rqqqk 261 1 One one CD cord-104269-9r7rqqqk 261 2 signal signal NN cord-104269-9r7rqqqk 261 3 involves involve VBZ cord-104269-9r7rqqqk 261 4 Leu Leu NNP cord-104269-9r7rqqqk 261 5 7 7 CD cord-104269-9r7rqqqk 261 6 and and CC cord-104269-9r7rqqqk 261 7 Ile Ile NNP cord-104269-9r7rqqqk 261 8 s s NNP cord-104269-9r7rqqqk 261 9 and and CC cord-104269-9r7rqqqk 261 10 appears appear VBZ cord-104269-9r7rqqqk 261 11 to to TO cord-104269-9r7rqqqk 261 12 be be VB cord-104269-9r7rqqqk 261 13 analogous analogous JJ cord-104269-9r7rqqqk 261 14 to to IN cord-104269-9r7rqqqk 261 15 the the DT cord-104269-9r7rqqqk 261 16 di di NN cord-104269-9r7rqqqk 261 17 - - HYPH cord-104269-9r7rqqqk 261 18 leucine leucine JJ cord-104269-9r7rqqqk 261 19 lysosomal lysosomal NN cord-104269-9r7rqqqk 261 20 targeting target VBG cord-104269-9r7rqqqk 261 21 signal signal NN cord-104269-9r7rqqqk 261 22 identified identify VBN cord-104269-9r7rqqqk 261 23 in in IN cord-104269-9r7rqqqk 261 24 the the DT cord-104269-9r7rqqqk 261 25 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 261 26 domain domain NN cord-104269-9r7rqqqk 261 27 of of IN cord-104269-9r7rqqqk 261 28 the the DT cord-104269-9r7rqqqk 261 29 CD3"y CD3"y NNP cord-104269-9r7rqqqk 261 30 chain chain NN cord-104269-9r7rqqqk 261 31 ( ( -LRB- cord-104269-9r7rqqqk 261 32 Letourneur Letourneur NNP cord-104269-9r7rqqqk 261 33 and and CC cord-104269-9r7rqqqk 261 34 Klausner Klausner NNP cord-104269-9r7rqqqk 261 35 , , , cord-104269-9r7rqqqk 261 36 1992 1992 CD cord-104269-9r7rqqqk 261 37 ) ) -RRB- cord-104269-9r7rqqqk 261 38 . . . cord-104269-9r7rqqqk 262 1 Deletion deletion NN cord-104269-9r7rqqqk 262 2 of of IN cord-104269-9r7rqqqk 262 3 residues residue NNS cord-104269-9r7rqqqk 262 4 2 2 CD cord-104269-9r7rqqqk 262 5 - - SYM cord-104269-9r7rqqqk 262 6 5 5 CD cord-104269-9r7rqqqk 262 7 from from IN cord-104269-9r7rqqqk 262 8 the the DT cord-104269-9r7rqqqk 262 9 Icr Icr NNP cord-104269-9r7rqqqk 262 10 chimera chimera NN cord-104269-9r7rqqqk 262 11 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 262 12 tall tall JJ cord-104269-9r7rqqqk 262 13 reduces reduce VBZ cord-104269-9r7rqqqk 262 14 the the DT cord-104269-9r7rqqqk 262 15 internalization internalization NN cord-104269-9r7rqqqk 262 16 activity activity NN cord-104269-9r7rqqqk 262 17 to to IN cord-104269-9r7rqqqk 262 18 , , , cord-104269-9r7rqqqk 262 19 ~50 ~50 NFP cord-104269-9r7rqqqk 262 20 % % NN cord-104269-9r7rqqqk 262 21 , , , cord-104269-9r7rqqqk 262 22 implying imply VBG cord-104269-9r7rqqqk 262 23 that that IN cord-104269-9r7rqqqk 262 24 , , , cord-104269-9r7rqqqk 262 25 as as IN cord-104269-9r7rqqqk 262 26 for for IN cord-104269-9r7rqqqk 262 27 the the DT cord-104269-9r7rqqqk 262 28 di di NN cord-104269-9r7rqqqk 262 29 - - HYPH cord-104269-9r7rqqqk 262 30 leucine leucine JJ cord-104269-9r7rqqqk 262 31 signal signal NN cord-104269-9r7rqqqk 262 32 of of IN cord-104269-9r7rqqqk 262 33 the the DT cord-104269-9r7rqqqk 262 34 CD33 CD33 NNP cord-104269-9r7rqqqk 262 35 , , , cord-104269-9r7rqqqk 262 36 chain chain NN cord-104269-9r7rqqqk 262 37 ( ( -LRB- cord-104269-9r7rqqqk 262 38 Letourneur Letourneur NNP cord-104269-9r7rqqqk 262 39 and and CC cord-104269-9r7rqqqk 262 40 Klausner Klausner NNP cord-104269-9r7rqqqk 262 41 , , , cord-104269-9r7rqqqk 262 42 1992 1992 CD cord-104269-9r7rqqqk 262 43 ) ) -RRB- cord-104269-9r7rqqqk 262 44 , , , cord-104269-9r7rqqqk 262 45 residues residue NNS cord-104269-9r7rqqqk 262 46 to to IN cord-104269-9r7rqqqk 262 47 the the DT cord-104269-9r7rqqqk 262 48 amino amino NN cord-104269-9r7rqqqk 262 49 - - HYPH cord-104269-9r7rqqqk 262 50 terminal terminal NN cord-104269-9r7rqqqk 262 51 side side NN cord-104269-9r7rqqqk 262 52 of of IN cord-104269-9r7rqqqk 262 53 Leuqle Leuqle NNP cord-104269-9r7rqqqk 262 54 s s NNP cord-104269-9r7rqqqk 262 55 are be VBP cord-104269-9r7rqqqk 262 56 required require VBN cord-104269-9r7rqqqk 262 57 for for IN cord-104269-9r7rqqqk 262 58 activity activity NN cord-104269-9r7rqqqk 262 59 . . . cord-104269-9r7rqqqk 263 1 The the DT cord-104269-9r7rqqqk 263 2 second second JJ cord-104269-9r7rqqqk 263 3 sorting sorting NN cord-104269-9r7rqqqk 263 4 signal signal NN cord-104269-9r7rqqqk 263 5 requires require VBZ cord-104269-9r7rqqqk 263 6 prols prol NNS cord-104269-9r7rqqqk 263 7 , , , cord-104269-9r7rqqqk 263 8 Met16 Met16 NNP cord-104269-9r7rqqqk 263 9 , , , cord-104269-9r7rqqqk 263 10 and and CC cord-104269-9r7rqqqk 263 11 Leu Leu NNP cord-104269-9r7rqqqk 263 12 ~7 ~7 NNP cord-104269-9r7rqqqk 263 13 , , , cord-104269-9r7rqqqk 263 14 and and CC cord-104269-9r7rqqqk 263 15 , , , cord-104269-9r7rqqqk 263 16 therefore therefore RB cord-104269-9r7rqqqk 263 17 , , , cord-104269-9r7rqqqk 263 18 differs differ VBZ cord-104269-9r7rqqqk 263 19 significantly significantly RB cord-104269-9r7rqqqk 263 20 from from IN cord-104269-9r7rqqqk 263 21 the the DT cord-104269-9r7rqqqk 263 22 dileucine dileucine NN cord-104269-9r7rqqqk 263 23 and and CC cord-104269-9r7rqqqk 263 24 leucine leucine NN cord-104269-9r7rqqqk 263 25 - - HYPH cord-104269-9r7rqqqk 263 26 isoleucine isoleucine JJ cord-104269-9r7rqqqk 263 27 motifs motif NNS cord-104269-9r7rqqqk 263 28 identified identify VBD cord-104269-9r7rqqqk 263 29 previously previously RB cord-104269-9r7rqqqk 263 30 ( ( -LRB- cord-104269-9r7rqqqk 263 31 Letourner Letourner NNP cord-104269-9r7rqqqk 263 32 and and CC cord-104269-9r7rqqqk 263 33 Klausner Klausner NNP cord-104269-9r7rqqqk 263 34 , , , cord-104269-9r7rqqqk 263 35 1992 1992 CD cord-104269-9r7rqqqk 263 36 ; ; : cord-104269-9r7rqqqk 263 37 Johnson Johnson NNP cord-104269-9r7rqqqk 263 38 and and CC cord-104269-9r7rqqqk 263 39 Kornfeld Kornfeld NNP cord-104269-9r7rqqqk 263 40 , , , cord-104269-9r7rqqqk 263 41 1992a 1992a CD cord-104269-9r7rqqqk 263 42 , , , cord-104269-9r7rqqqk 263 43 b b LS cord-104269-9r7rqqqk 263 44 ; ; : cord-104269-9r7rqqqk 263 45 Ogata Ogata NNP cord-104269-9r7rqqqk 263 46 and and CC cord-104269-9r7rqqqk 263 47 Fukuda Fukuda NNP cord-104269-9r7rqqqk 263 48 , , , cord-104269-9r7rqqqk 263 49 1994 1994 CD cord-104269-9r7rqqqk 263 50 ) ) -RRB- cord-104269-9r7rqqqk 263 51 . . . cord-104269-9r7rqqqk 264 1 Nevertheless nevertheless RB cord-104269-9r7rqqqk 264 2 , , , cord-104269-9r7rqqqk 264 3 all all PDT cord-104269-9r7rqqqk 264 4 these these DT cord-104269-9r7rqqqk 264 5 signals signal NNS cord-104269-9r7rqqqk 264 6 , , , cord-104269-9r7rqqqk 264 7 including include VBG cord-104269-9r7rqqqk 264 8 both both DT cord-104269-9r7rqqqk 264 9 11 11 CD cord-104269-9r7rqqqk 264 10 signals signal NNS cord-104269-9r7rqqqk 264 11 , , , cord-104269-9r7rqqqk 264 12 contain contain VBP cord-104269-9r7rqqqk 264 13 two two CD cord-104269-9r7rqqqk 264 14 adjacent adjacent JJ cord-104269-9r7rqqqk 264 15 large large JJ cord-104269-9r7rqqqk 264 16 nonaromatic nonaromatic JJ cord-104269-9r7rqqqk 264 17 hydrophobic hydrophobic JJ cord-104269-9r7rqqqk 264 18 residues residue NNS cord-104269-9r7rqqqk 264 19 and and CC cord-104269-9r7rqqqk 264 20 may may MD cord-104269-9r7rqqqk 264 21 , , , cord-104269-9r7rqqqk 264 22 therefore therefore RB cord-104269-9r7rqqqk 264 23 , , , cord-104269-9r7rqqqk 264 24 represent represent VBP cord-104269-9r7rqqqk 264 25 a a DT cord-104269-9r7rqqqk 264 26 family family NN cord-104269-9r7rqqqk 264 27 of of IN cord-104269-9r7rqqqk 264 28 related relate VBN cord-104269-9r7rqqqk 264 29 structural structural JJ cord-104269-9r7rqqqk 264 30 motifs motif NNS cord-104269-9r7rqqqk 264 31 specified specify VBN cord-104269-9r7rqqqk 264 32 by by IN cord-104269-9r7rqqqk 264 33 short short JJ cord-104269-9r7rqqqk 264 34 linear linear JJ cord-104269-9r7rqqqk 264 35 arrays array NNS cord-104269-9r7rqqqk 264 36 of of IN cord-104269-9r7rqqqk 264 37 amino amino JJ cord-104269-9r7rqqqk 264 38 acids acid NNS cord-104269-9r7rqqqk 264 39 that that WDT cord-104269-9r7rqqqk 264 40 differ differ VBP cord-104269-9r7rqqqk 264 41 in in IN cord-104269-9r7rqqqk 264 42 specific specific JJ cord-104269-9r7rqqqk 264 43 sequence sequence NN cord-104269-9r7rqqqk 264 44 , , , cord-104269-9r7rqqqk 264 45 analogous analogous JJ cord-104269-9r7rqqqk 264 46 to to IN cord-104269-9r7rqqqk 264 47 tyrosine tyrosine NN cord-104269-9r7rqqqk 264 48 - - HYPH cord-104269-9r7rqqqk 264 49 based base VBN cord-104269-9r7rqqqk 264 50 signals signal NNS cord-104269-9r7rqqqk 265 1 ( ( -LRB- cord-104269-9r7rqqqk 265 2 Trowbridge Trowbridge NNP cord-104269-9r7rqqqk 265 3 et et NNP cord-104269-9r7rqqqk 265 4 al al NNP cord-104269-9r7rqqqk 265 5 . . NNP cord-104269-9r7rqqqk 265 6 , , , cord-104269-9r7rqqqk 265 7 1993 1993 CD cord-104269-9r7rqqqk 265 8 ) ) -RRB- cord-104269-9r7rqqqk 265 9 . . . cord-104269-9r7rqqqk 266 1 Additional additional JJ cord-104269-9r7rqqqk 266 2 mutagenesis mutagenesis NN cord-104269-9r7rqqqk 266 3 is be VBZ cord-104269-9r7rqqqk 266 4 required require VBN cord-104269-9r7rqqqk 266 5 , , , cord-104269-9r7rqqqk 266 6 however however RB cord-104269-9r7rqqqk 266 7 , , , cord-104269-9r7rqqqk 266 8 to to TO cord-104269-9r7rqqqk 266 9 define define VB cord-104269-9r7rqqqk 266 10 these these DT cord-104269-9r7rqqqk 266 11 sorting sorting NN cord-104269-9r7rqqqk 266 12 signals signal NNS cord-104269-9r7rqqqk 266 13 more more RBR cord-104269-9r7rqqqk 266 14 precisely precisely RB cord-104269-9r7rqqqk 266 15 , , , cord-104269-9r7rqqqk 266 16 to to TO cord-104269-9r7rqqqk 266 17 identify identify VB cord-104269-9r7rqqqk 266 18 any any DT cord-104269-9r7rqqqk 266 19 common common JJ cord-104269-9r7rqqqk 266 20 features feature NNS cord-104269-9r7rqqqk 266 21 they -PRON- PRP cord-104269-9r7rqqqk 266 22 may may MD cord-104269-9r7rqqqk 266 23 share share VB cord-104269-9r7rqqqk 266 24 , , , cord-104269-9r7rqqqk 266 25 and and CC cord-104269-9r7rqqqk 266 26 to to TO cord-104269-9r7rqqqk 266 27 delineate delineate VB cord-104269-9r7rqqqk 266 28 any any DT cord-104269-9r7rqqqk 266 29 differences difference NNS cord-104269-9r7rqqqk 266 30 between between IN cord-104269-9r7rqqqk 266 31 signals signal NNS cord-104269-9r7rqqqk 266 32 recognized recognize VBN cord-104269-9r7rqqqk 266 33 in in IN cord-104269-9r7rqqqk 266 34 the the DT cord-104269-9r7rqqqk 266 35 trans trans NNP cord-104269-9r7rqqqk 266 36 - - NNP cord-104269-9r7rqqqk 266 37 Golgi Golgi NNP cord-104269-9r7rqqqk 266 38 or or CC cord-104269-9r7rqqqk 266 39 at at IN cord-104269-9r7rqqqk 266 40 the the DT cord-104269-9r7rqqqk 266 41 plasma plasma NN cord-104269-9r7rqqqk 266 42 membrane membrane NN cord-104269-9r7rqqqk 266 43 . . . cord-104269-9r7rqqqk 267 1 At at IN cord-104269-9r7rqqqk 267 2 present present NN cord-104269-9r7rqqqk 267 3 , , , cord-104269-9r7rqqqk 267 4 we -PRON- PRP cord-104269-9r7rqqqk 267 5 believe believe VBP cord-104269-9r7rqqqk 267 6 it -PRON- PRP cord-104269-9r7rqqqk 267 7 most most RBS cord-104269-9r7rqqqk 267 8 appropriate appropriate JJ cord-104269-9r7rqqqk 267 9 to to TO cord-104269-9r7rqqqk 267 10 refer refer VB cord-104269-9r7rqqqk 267 11 to to IN cord-104269-9r7rqqqk 267 12 them -PRON- PRP cord-104269-9r7rqqqk 267 13 as as IN cord-104269-9r7rqqqk 267 14 non non JJ cord-104269-9r7rqqqk 267 15 - - JJ cord-104269-9r7rqqqk 267 16 tyrosine tyrosine NN cord-104269-9r7rqqqk 267 17 - - HYPH cord-104269-9r7rqqqk 267 18 based base VBN cord-104269-9r7rqqqk 267 19 signals signal NNS cord-104269-9r7rqqqk 267 20 , , , cord-104269-9r7rqqqk 267 21 a a DT cord-104269-9r7rqqqk 267 22 neutral neutral JJ cord-104269-9r7rqqqk 267 23 term term NN cord-104269-9r7rqqqk 267 24 that that WDT cord-104269-9r7rqqqk 267 25 serves serve VBZ cord-104269-9r7rqqqk 267 26 to to TO cord-104269-9r7rqqqk 267 27 distinguish distinguish VB cord-104269-9r7rqqqk 267 28 them -PRON- PRP cord-104269-9r7rqqqk 267 29 from from IN cord-104269-9r7rqqqk 267 30 tyrosine tyrosine NN cord-104269-9r7rqqqk 267 31 - - HYPH cord-104269-9r7rqqqk 267 32 based base VBN cord-104269-9r7rqqqk 267 33 signals signal NNS cord-104269-9r7rqqqk 267 34 . . . cord-104269-9r7rqqqk 268 1 Our -PRON- PRP$ cord-104269-9r7rqqqk 268 2 results result NNS cord-104269-9r7rqqqk 268 3 are be VBP cord-104269-9r7rqqqk 268 4 consistent consistent JJ cord-104269-9r7rqqqk 268 5 with with IN cord-104269-9r7rqqqk 268 6 those those DT cord-104269-9r7rqqqk 268 7 of of IN cord-104269-9r7rqqqk 268 8 Bakke Bakke NNP cord-104269-9r7rqqqk 268 9 and and CC cord-104269-9r7rqqqk 268 10 Dobberstein Dobberstein NNP cord-104269-9r7rqqqk 268 11 ( ( -LRB- cord-104269-9r7rqqqk 268 12 1990 1990 CD cord-104269-9r7rqqqk 268 13 ) ) -RRB- cord-104269-9r7rqqqk 268 14 , , , cord-104269-9r7rqqqk 268 15 who who WP cord-104269-9r7rqqqk 268 16 identified identify VBD cord-104269-9r7rqqqk 268 17 amino amino JJ cord-104269-9r7rqqqk 268 18 acids acid NNS cord-104269-9r7rqqqk 268 19 12 12 CD cord-104269-9r7rqqqk 268 20 - - SYM cord-104269-9r7rqqqk 268 21 15 15 CD cord-104269-9r7rqqqk 268 22 in in IN cord-104269-9r7rqqqk 268 23 the the DT cord-104269-9r7rqqqk 268 24 Ii Ii NNP cord-104269-9r7rqqqk 268 25 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 268 26 tail tail NN cord-104269-9r7rqqqk 268 27 as as IN cord-104269-9r7rqqqk 268 28 important important JJ cord-104269-9r7rqqqk 268 29 for for IN cord-104269-9r7rqqqk 268 30 targeting target VBG cord-104269-9r7rqqqk 268 31 Ii Ii NNP cord-104269-9r7rqqqk 268 32 to to IN cord-104269-9r7rqqqk 268 33 the the DT cord-104269-9r7rqqqk 268 34 endocytic endocytic JJ cord-104269-9r7rqqqk 268 35 pathway pathway NN cord-104269-9r7rqqqk 268 36 . . . cord-104269-9r7rqqqk 269 1 Lotteau Lotteau NNP cord-104269-9r7rqqqk 269 2 et et NNP cord-104269-9r7rqqqk 269 3 al al NNP cord-104269-9r7rqqqk 269 4 . . . cord-104269-9r7rqqqk 270 1 ( ( -LRB- cord-104269-9r7rqqqk 270 2 1990 1990 CD cord-104269-9r7rqqqk 270 3 ) ) -RRB- cord-104269-9r7rqqqk 270 4 also also RB cord-104269-9r7rqqqk 270 5 concluded conclude VBD cord-104269-9r7rqqqk 270 6 that that IN cord-104269-9r7rqqqk 270 7 a a DT cord-104269-9r7rqqqk 270 8 sorting sorting NN cord-104269-9r7rqqqk 270 9 signal signal NN cord-104269-9r7rqqqk 270 10 was be VBD cord-104269-9r7rqqqk 270 11 located locate VBN cord-104269-9r7rqqqk 270 12 within within IN cord-104269-9r7rqqqk 270 13 residues residue NNS cord-104269-9r7rqqqk 270 14 10 10 CD cord-104269-9r7rqqqk 270 15 - - SYM cord-104269-9r7rqqqk 270 16 16 16 CD cord-104269-9r7rqqqk 270 17 of of IN cord-104269-9r7rqqqk 270 18 the the DT cord-104269-9r7rqqqk 270 19 Ii Ii NNP cord-104269-9r7rqqqk 270 20 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 270 21 tail tail NN cord-104269-9r7rqqqk 270 22 . . . cord-104269-9r7rqqqk 271 1 The the DT cord-104269-9r7rqqqk 271 2 distal distal JJ cord-104269-9r7rqqqk 271 3 signal signal NN cord-104269-9r7rqqqk 271 4 involving involve VBG cord-104269-9r7rqqqk 271 5 Leuqle Leuqle NNP cord-104269-9r7rqqqk 271 6 s s POS cord-104269-9r7rqqqk 271 7 was be VBD cord-104269-9r7rqqqk 271 8 not not RB cord-104269-9r7rqqqk 271 9 identified identify VBN cord-104269-9r7rqqqk 271 10 in in IN cord-104269-9r7rqqqk 271 11 either either DT cord-104269-9r7rqqqk 271 12 of of IN cord-104269-9r7rqqqk 271 13 these these DT cord-104269-9r7rqqqk 271 14 studies study NNS cord-104269-9r7rqqqk 271 15 , , , cord-104269-9r7rqqqk 271 16 however however RB cord-104269-9r7rqqqk 271 17 ( ( -LRB- cord-104269-9r7rqqqk 271 18 see see VB cord-104269-9r7rqqqk 271 19 also also RB cord-104269-9r7rqqqk 271 20 Romagnoli Romagnoli NNP cord-104269-9r7rqqqk 271 21 et et FW cord-104269-9r7rqqqk 271 22 al al NNP cord-104269-9r7rqqqk 271 23 . . NNP cord-104269-9r7rqqqk 271 24 , , , cord-104269-9r7rqqqk 271 25 1993 1993 CD cord-104269-9r7rqqqk 271 26 ) ) -RRB- cord-104269-9r7rqqqk 271 27 , , , cord-104269-9r7rqqqk 271 28 illustrating illustrate VBG cord-104269-9r7rqqqk 271 29 the the DT cord-104269-9r7rqqqk 271 30 difficulty difficulty NN cord-104269-9r7rqqqk 271 31 of of IN cord-104269-9r7rqqqk 271 32 detecting detect VBG cord-104269-9r7rqqqk 271 33 multiple multiple JJ cord-104269-9r7rqqqk 271 34 signals signal NNS cord-104269-9r7rqqqk 271 35 without without IN cord-104269-9r7rqqqk 271 36 quantitative quantitative JJ cord-104269-9r7rqqqk 271 37 assays assay NNS cord-104269-9r7rqqqk 271 38 . . . cord-104269-9r7rqqqk 272 1 Recently recently RB cord-104269-9r7rqqqk 272 2 , , , cord-104269-9r7rqqqk 272 3 in in IN cord-104269-9r7rqqqk 272 4 agreement agreement NN cord-104269-9r7rqqqk 272 5 with with IN cord-104269-9r7rqqqk 272 6 our -PRON- PRP$ cord-104269-9r7rqqqk 272 7 data datum NNS cord-104269-9r7rqqqk 272 8 , , , cord-104269-9r7rqqqk 272 9 Pieters Pieters NNP cord-104269-9r7rqqqk 272 10 et et NNP cord-104269-9r7rqqqk 272 11 al al NNP cord-104269-9r7rqqqk 272 12 . . . cord-104269-9r7rqqqk 273 1 ( ( -LRB- cord-104269-9r7rqqqk 273 2 1993 1993 CD cord-104269-9r7rqqqk 273 3 ) ) -RRB- cord-104269-9r7rqqqk 273 4 have have VBP cord-104269-9r7rqqqk 273 5 independently independently RB cord-104269-9r7rqqqk 273 6 concluded conclude VBN cord-104269-9r7rqqqk 273 7 that that IN cord-104269-9r7rqqqk 273 8 the the DT cord-104269-9r7rqqqk 273 9 Ii Ii NNP cord-104269-9r7rqqqk 273 10 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 273 11 tail tail NN cord-104269-9r7rqqqk 273 12 contains contain VBZ cord-104269-9r7rqqqk 273 13 two two CD cord-104269-9r7rqqqk 273 14 sorting sorting NN cord-104269-9r7rqqqk 273 15 signals signal NNS cord-104269-9r7rqqqk 273 16 , , , cord-104269-9r7rqqqk 273 17 one one CD cord-104269-9r7rqqqk 273 18 involving involve VBG cord-104269-9r7rqqqk 273 19 LeuTIle LeuTIle NNP cord-104269-9r7rqqqk 273 20 8 8 CD cord-104269-9r7rqqqk 273 21 , , , cord-104269-9r7rqqqk 274 1 the the DT cord-104269-9r7rqqqk 274 2 other other JJ cord-104269-9r7rqqqk 274 3 localized localize VBN cord-104269-9r7rqqqk 274 4 to to IN cord-104269-9r7rqqqk 274 5 residues residue NNS cord-104269-9r7rqqqk 274 6 12 12 CD cord-104269-9r7rqqqk 274 7 - - SYM cord-104269-9r7rqqqk 274 8 29 29 CD cord-104269-9r7rqqqk 274 9 . . . cord-104269-9r7rqqqk 275 1 The the DT cord-104269-9r7rqqqk 275 2 extent extent NN cord-104269-9r7rqqqk 275 3 to to TO cord-104269-9r7rqqqk 275 4 which which WDT cord-104269-9r7rqqqk 275 5 newly newly RB cord-104269-9r7rqqqk 275 6 synthesized synthesize VBN cord-104269-9r7rqqqk 275 7 11 11 CD cord-104269-9r7rqqqk 275 8 molecules molecule NNS cord-104269-9r7rqqqk 275 9 , , , cord-104269-9r7rqqqk 275 10 alone alone RB cord-104269-9r7rqqqk 275 11 or or CC cord-104269-9r7rqqqk 275 12 complexed complexed JJ cord-104269-9r7rqqqk 275 13 to to IN cord-104269-9r7rqqqk 275 14 MHC MHC NNP cord-104269-9r7rqqqk 275 15 class class NN cord-104269-9r7rqqqk 275 16 II II NNP cord-104269-9r7rqqqk 275 17 c~/~ c~/~ NNS cord-104269-9r7rqqqk 275 18 dimers dimer NNS cord-104269-9r7rqqqk 275 19 , , , cord-104269-9r7rqqqk 275 20 traffic traffic NN cord-104269-9r7rqqqk 275 21 to to IN cord-104269-9r7rqqqk 275 22 the the DT cord-104269-9r7rqqqk 275 23 endocytic endocytic JJ cord-104269-9r7rqqqk 275 24 pathway pathway NN cord-104269-9r7rqqqk 275 25 by by IN cord-104269-9r7rqqqk 275 26 an an DT cord-104269-9r7rqqqk 275 27 indirect indirect JJ cord-104269-9r7rqqqk 275 28 route route NN cord-104269-9r7rqqqk 275 29 via via IN cord-104269-9r7rqqqk 275 30 the the DT cord-104269-9r7rqqqk 275 31 cell cell NN cord-104269-9r7rqqqk 275 32 surface surface NN cord-104269-9r7rqqqk 275 33 has have VBZ cord-104269-9r7rqqqk 275 34 been be VBN cord-104269-9r7rqqqk 275 35 a a DT cord-104269-9r7rqqqk 275 36 matter matter NN cord-104269-9r7rqqqk 275 37 of of IN cord-104269-9r7rqqqk 275 38 debate debate NN cord-104269-9r7rqqqk 275 39 ( ( -LRB- cord-104269-9r7rqqqk 275 40 Peters Peters NNP cord-104269-9r7rqqqk 275 41 et et NNP cord-104269-9r7rqqqk 275 42 al al NNP cord-104269-9r7rqqqk 275 43 . . NNP cord-104269-9r7rqqqk 275 44 , , , cord-104269-9r7rqqqk 275 45 1991 1991 CD cord-104269-9r7rqqqk 275 46 ; ; : cord-104269-9r7rqqqk 275 47 Pieters Pieters NNP cord-104269-9r7rqqqk 275 48 et et NNP cord-104269-9r7rqqqk 275 49 al al NNP cord-104269-9r7rqqqk 275 50 . . NNP cord-104269-9r7rqqqk 275 51 , , , cord-104269-9r7rqqqk 275 52 1991 1991 CD cord-104269-9r7rqqqk 275 53 ; ; : cord-104269-9r7rqqqk 275 54 Roche Roche NNP cord-104269-9r7rqqqk 275 55 et et NNP cord-104269-9r7rqqqk 275 56 al al NNP cord-104269-9r7rqqqk 275 57 . . NNP cord-104269-9r7rqqqk 275 58 , , , cord-104269-9r7rqqqk 275 59 1993 1993 CD cord-104269-9r7rqqqk 275 60 ) ) -RRB- cord-104269-9r7rqqqk 275 61 . . . cord-104269-9r7rqqqk 276 1 Ii Ii NNP cord-104269-9r7rqqqk 276 2 - - HYPH cord-104269-9r7rqqqk 276 3 TR TR NNP cord-104269-9r7rqqqk 276 4 chimeras chimera NNS cord-104269-9r7rqqqk 276 5 trafficking traffic VBG cord-104269-9r7rqqqk 276 6 to to IN cord-104269-9r7rqqqk 276 7 the the DT cord-104269-9r7rqqqk 276 8 plasma plasma NN cord-104269-9r7rqqqk 276 9 membrane membrane NN cord-104269-9r7rqqqk 276 10 can can MD cord-104269-9r7rqqqk 276 11 be be VB cord-104269-9r7rqqqk 276 12 quantitated quantitate VBN cord-104269-9r7rqqqk 276 13 because because IN cord-104269-9r7rqqqk 276 14 receptors receptor NNS cord-104269-9r7rqqqk 276 15 appearing appear VBG cord-104269-9r7rqqqk 276 16 transiently transiently RB cord-104269-9r7rqqqk 276 17 at at IN cord-104269-9r7rqqqk 276 18 the the DT cord-104269-9r7rqqqk 276 19 cell cell NN cord-104269-9r7rqqqk 276 20 surface surface NN cord-104269-9r7rqqqk 276 21 can can MD cord-104269-9r7rqqqk 276 22 be be VB cord-104269-9r7rqqqk 276 23 labeled label VBN cord-104269-9r7rqqqk 276 24 with with IN cord-104269-9r7rqqqk 276 25 exogenous exogenous JJ cord-104269-9r7rqqqk 276 26 Tf Tf NNP cord-104269-9r7rqqqk 276 27 . . . cord-104269-9r7rqqqk 277 1 Thus thus RB cord-104269-9r7rqqqk 277 2 , , , cord-104269-9r7rqqqk 277 3 the the DT cord-104269-9r7rqqqk 277 4 surface surface NN cord-104269-9r7rqqqk 277 5 - - HYPH cord-104269-9r7rqqqk 277 6 accessible accessible JJ cord-104269-9r7rqqqk 277 7 pools pool NNS cord-104269-9r7rqqqk 277 8 of of IN cord-104269-9r7rqqqk 277 9 the the DT cord-104269-9r7rqqqk 277 10 Ii Ii NNP cord-104269-9r7rqqqk 277 11 - - HYPH cord-104269-9r7rqqqk 277 12 TR TR NNP cord-104269-9r7rqqqk 277 13 chimeras chimera NNS cord-104269-9r7rqqqk 277 14 can can MD cord-104269-9r7rqqqk 277 15 be be VB cord-104269-9r7rqqqk 277 16 determined determine VBN cord-104269-9r7rqqqk 277 17 by by IN cord-104269-9r7rqqqk 277 18 measuring measure VBG cord-104269-9r7rqqqk 277 19 ~25I ~25I NNS cord-104269-9r7rqqqk 277 20 - - HYPH cord-104269-9r7rqqqk 277 21 labeled label VBN cord-104269-9r7rqqqk 277 22 Tf Tf NNP cord-104269-9r7rqqqk 277 23 bound bind VBN cord-104269-9r7rqqqk 277 24 to to TO cord-104269-9r7rqqqk 277 25 surface surface VB cord-104269-9r7rqqqk 277 26 and and CC cord-104269-9r7rqqqk 277 27 internalized internalize VBN cord-104269-9r7rqqqk 277 28 chimeric chimeric JJ cord-104269-9r7rqqqk 277 29 receptors receptor NNS cord-104269-9r7rqqqk 277 30 under under IN cord-104269-9r7rqqqk 277 31 steady steady JJ cord-104269-9r7rqqqk 277 32 - - HYPH cord-104269-9r7rqqqk 277 33 state state NN cord-104269-9r7rqqqk 277 34 conditions condition NNS cord-104269-9r7rqqqk 277 35 . . . cord-104269-9r7rqqqk 278 1 The the DT cord-104269-9r7rqqqk 278 2 surface surface NN cord-104269-9r7rqqqk 278 3 - - HYPH cord-104269-9r7rqqqk 278 4 accessible accessible JJ cord-104269-9r7rqqqk 278 5 pool pool NN cord-104269-9r7rqqqk 278 6 of of IN cord-104269-9r7rqqqk 278 7 the the DT cord-104269-9r7rqqqk 278 8 Iicr+rM iicr+rm NN cord-104269-9r7rqqqk 278 9 chimera chimera NN cord-104269-9r7rqqqk 278 10 was be VBD cord-104269-9r7rqqqk 278 11 only only RB cord-104269-9r7rqqqk 278 12 19 19 CD cord-104269-9r7rqqqk 278 13 % % NN cord-104269-9r7rqqqk 278 14 of of IN cord-104269-9r7rqqqk 278 15 the the DT cord-104269-9r7rqqqk 278 16 wild wild JJ cord-104269-9r7rqqqk 278 17 - - HYPH cord-104269-9r7rqqqk 278 18 type type NN cord-104269-9r7rqqqk 278 19 TR tr NN cord-104269-9r7rqqqk 278 20 pool pool NN cord-104269-9r7rqqqk 278 21 despite despite IN cord-104269-9r7rqqqk 278 22 the the DT cord-104269-9r7rqqqk 278 23 fact fact NN cord-104269-9r7rqqqk 278 24 that that IN cord-104269-9r7rqqqk 278 25 the the DT cord-104269-9r7rqqqk 278 26 , , , cord-104269-9r7rqqqk 278 27 , , , cord-104269-9r7rqqqk 278 28 o o LS cord-104269-9r7rqqqk 278 29 8-fold 8-fold CD cord-104269-9r7rqqqk 278 30 increase increase NN cord-104269-9r7rqqqk 278 31 in in IN cord-104269-9r7rqqqk 278 32 the the DT cord-104269-9r7rqqqk 278 33 degradation degradation NN cord-104269-9r7rqqqk 278 34 rate rate NN cord-104269-9r7rqqqk 278 35 of of IN cord-104269-9r7rqqqk 278 36 the the DT cord-104269-9r7rqqqk 278 37 Iicr+rM iicr+rm NN cord-104269-9r7rqqqk 278 38 surface surface NN cord-104269-9r7rqqqk 278 39 - - HYPH cord-104269-9r7rqqqk 278 40 accessible accessible JJ cord-104269-9r7rqqqk 278 41 pool pool NN cord-104269-9r7rqqqk 278 42 is be VBZ cord-104269-9r7rqqqk 278 43 offset offset VBN cord-104269-9r7rqqqk 278 44 by by IN cord-104269-9r7rqqqk 278 45 a a DT cord-104269-9r7rqqqk 278 46 similar similar JJ cord-104269-9r7rqqqk 278 47 increased increase VBN cord-104269-9r7rqqqk 278 48 rate rate NN cord-104269-9r7rqqqk 278 49 of of IN cord-104269-9r7rqqqk 278 50 biosynthesis biosynthesis NN cord-104269-9r7rqqqk 278 51 relative relative JJ cord-104269-9r7rqqqk 278 52 to to IN cord-104269-9r7rqqqk 278 53 wild wild JJ cord-104269-9r7rqqqk 278 54 - - HYPH cord-104269-9r7rqqqk 278 55 type type NN cord-104269-9r7rqqqk 278 56 TR tr NN cord-104269-9r7rqqqk 278 57 ( ( -LRB- cord-104269-9r7rqqqk 278 58 see see VB cord-104269-9r7rqqqk 278 59 Materials material NNS cord-104269-9r7rqqqk 278 60 and and CC cord-104269-9r7rqqqk 278 61 Methods method NNS cord-104269-9r7rqqqk 278 62 for for IN cord-104269-9r7rqqqk 278 63 details detail NNS cord-104269-9r7rqqqk 278 64 of of IN cord-104269-9r7rqqqk 278 65 the the DT cord-104269-9r7rqqqk 278 66 calculation calculation NN cord-104269-9r7rqqqk 278 67 ) ) -RRB- cord-104269-9r7rqqqk 278 68 . . . cord-104269-9r7rqqqk 279 1 Assuming assume VBG cord-104269-9r7rqqqk 279 2 that that IN cord-104269-9r7rqqqk 279 3 essentially essentially RB cord-104269-9r7rqqqk 279 4 all all DT cord-104269-9r7rqqqk 279 5 wild wild JJ cord-104269-9r7rqqqk 279 6 - - HYPH cord-104269-9r7rqqqk 279 7 type type NN cord-104269-9r7rqqqk 279 8 TRs tr NNS cord-104269-9r7rqqqk 279 9 bind bind VBP cord-104269-9r7rqqqk 279 10 Tf Tf NNP cord-104269-9r7rqqqk 279 11 under under IN cord-104269-9r7rqqqk 279 12 steady steady JJ cord-104269-9r7rqqqk 279 13 - - HYPH cord-104269-9r7rqqqk 279 14 state state NN cord-104269-9r7rqqqk 279 15 labeling labeling NN cord-104269-9r7rqqqk 279 16 conditions condition NNS cord-104269-9r7rqqqk 279 17 , , , cord-104269-9r7rqqqk 279 18 it -PRON- PRP cord-104269-9r7rqqqk 279 19 can can MD cord-104269-9r7rqqqk 279 20 be be VB cord-104269-9r7rqqqk 279 21 calculated calculate VBN cord-104269-9r7rqqqk 279 22 that that IN cord-104269-9r7rqqqk 279 23 only only RB cord-104269-9r7rqqqk 279 24 19 19 CD cord-104269-9r7rqqqk 279 25 % % NN cord-104269-9r7rqqqk 279 26 of of IN cord-104269-9r7rqqqk 279 27 the the DT cord-104269-9r7rqqqk 279 28 Iicr+rM iicr+rm NN cord-104269-9r7rqqqk 279 29 chimera chimera NN cord-104269-9r7rqqqk 279 30 traffics traffic VBZ cord-104269-9r7rqqqk 279 31 via via IN cord-104269-9r7rqqqk 279 32 the the DT cord-104269-9r7rqqqk 279 33 cell cell NN cord-104269-9r7rqqqk 279 34 surface surface NN cord-104269-9r7rqqqk 279 35 , , , cord-104269-9r7rqqqk 279 36 and and CC cord-104269-9r7rqqqk 279 37 , , , cord-104269-9r7rqqqk 279 38 therefore therefore RB cord-104269-9r7rqqqk 279 39 , , , cord-104269-9r7rqqqk 279 40 that that IN cord-104269-9r7rqqqk 279 41 > > XX cord-104269-9r7rqqqk 279 42 80 80 CD cord-104269-9r7rqqqk 279 43 % % NN cord-104269-9r7rqqqk 279 44 of of IN cord-104269-9r7rqqqk 279 45 the the DT cord-104269-9r7rqqqk 279 46 chimera chimera NN cord-104269-9r7rqqqk 279 47 must must MD cord-104269-9r7rqqqk 279 48 be be VB cord-104269-9r7rqqqk 279 49 delivered deliver VBN cord-104269-9r7rqqqk 279 50 by by IN cord-104269-9r7rqqqk 279 51 a a DT cord-104269-9r7rqqqk 279 52 direct direct JJ cord-104269-9r7rqqqk 279 53 intraceilular intraceilular JJ cord-104269-9r7rqqqk 279 54 route route NN cord-104269-9r7rqqqk 279 55 to to IN cord-104269-9r7rqqqk 279 56 the the DT cord-104269-9r7rqqqk 279 57 endocytic endocytic JJ cord-104269-9r7rqqqk 279 58 compartment compartment NN cord-104269-9r7rqqqk 279 59 where where WRB cord-104269-9r7rqqqk 279 60 it -PRON- PRP cord-104269-9r7rqqqk 279 61 is be VBZ cord-104269-9r7rqqqk 279 62 degraded degrade VBN cord-104269-9r7rqqqk 279 63 . . . cord-104269-9r7rqqqk 280 1 Similarly similarly RB cord-104269-9r7rqqqk 280 2 , , , cord-104269-9r7rqqqk 280 3 •50 •50 VBZ cord-104269-9r7rqqqk 280 4 % % NN cord-104269-9r7rqqqk 280 5 of of IN cord-104269-9r7rqqqk 280 6 the the DT cord-104269-9r7rqqqk 280 7 Iicr Iicr NNP cord-104269-9r7rqqqk 280 8 chimera chimera NN cord-104269-9r7rqqqk 280 9 is be VBZ cord-104269-9r7rqqqk 280 10 degraded degrade VBN cord-104269-9r7rqqqk 280 11 without without IN cord-104269-9r7rqqqk 280 12 ever ever RB cord-104269-9r7rqqqk 280 13 being be VBG cord-104269-9r7rqqqk 280 14 displayed display VBN cord-104269-9r7rqqqk 280 15 on on IN cord-104269-9r7rqqqk 280 16 the the DT cord-104269-9r7rqqqk 280 17 cell cell NN cord-104269-9r7rqqqk 280 18 surface surface NN cord-104269-9r7rqqqk 280 19 . . . cord-104269-9r7rqqqk 281 1 The the DT cord-104269-9r7rqqqk 281 2 lysosomal lysosomal JJ cord-104269-9r7rqqqk 281 3 membrane membrane NN cord-104269-9r7rqqqk 281 4 glycoprotein glycoprotein NN cord-104269-9r7rqqqk 281 5 , , , cord-104269-9r7rqqqk 281 6 Lgp Lgp NNP cord-104269-9r7rqqqk 281 7 - - HYPH cord-104269-9r7rqqqk 281 8 A A NNP cord-104269-9r7rqqqk 281 9 ( ( -LRB- cord-104269-9r7rqqqk 281 10 LEP100 LEP100 NNPS cord-104269-9r7rqqqk 281 11 , , , cord-104269-9r7rqqqk 281 12 LAMP-1 LAMP-1 NNP cord-104269-9r7rqqqk 281 13 ) ) -RRB- cord-104269-9r7rqqqk 281 14 , , , cord-104269-9r7rqqqk 281 15 also also RB cord-104269-9r7rqqqk 281 16 traffics traffic VBZ cord-104269-9r7rqqqk 281 17 along along IN cord-104269-9r7rqqqk 281 18 the the DT cord-104269-9r7rqqqk 281 19 endocytic endocytic JJ cord-104269-9r7rqqqk 281 20 pathway pathway NN cord-104269-9r7rqqqk 281 21 to to IN cord-104269-9r7rqqqk 281 22 the the DT cord-104269-9r7rqqqk 281 23 lysosome lysosome NN cord-104269-9r7rqqqk 281 24 by by IN cord-104269-9r7rqqqk 281 25 a a DT cord-104269-9r7rqqqk 281 26 direct direct JJ cord-104269-9r7rqqqk 281 27 intracellular intracellular JJ cord-104269-9r7rqqqk 281 28 route route NN cord-104269-9r7rqqqk 281 29 or or CC cord-104269-9r7rqqqk 281 30 via via IN cord-104269-9r7rqqqk 281 31 the the DT cord-104269-9r7rqqqk 281 32 cell cell NN cord-104269-9r7rqqqk 281 33 surface surface NN cord-104269-9r7rqqqk 281 34 , , , cord-104269-9r7rqqqk 282 1 although although IN cord-104269-9r7rqqqk 282 2 which which WDT cord-104269-9r7rqqqk 282 3 route route NN cord-104269-9r7rqqqk 282 4 is be VBZ cord-104269-9r7rqqqk 282 5 the the DT cord-104269-9r7rqqqk 282 6 major major JJ cord-104269-9r7rqqqk 282 7 pathway pathway NN cord-104269-9r7rqqqk 282 8 is be VBZ cord-104269-9r7rqqqk 282 9 less less RBR cord-104269-9r7rqqqk 282 10 clear clear JJ cord-104269-9r7rqqqk 282 11 and and CC cord-104269-9r7rqqqk 282 12 may may MD cord-104269-9r7rqqqk 282 13 depend depend VB cord-104269-9r7rqqqk 282 14 upon upon IN cord-104269-9r7rqqqk 282 15 expression expression NN cord-104269-9r7rqqqk 282 16 level level NN cord-104269-9r7rqqqk 282 17 ( ( -LRB- cord-104269-9r7rqqqk 282 18 Williarns Williarns NNP cord-104269-9r7rqqqk 282 19 and and CC cord-104269-9r7rqqqk 282 20 Fukuda Fukuda NNP cord-104269-9r7rqqqk 282 21 , , , cord-104269-9r7rqqqk 282 22 1990 1990 CD cord-104269-9r7rqqqk 282 23 ; ; : cord-104269-9r7rqqqk 283 1 Mathews Mathews NNP cord-104269-9r7rqqqk 283 2 et et NNP cord-104269-9r7rqqqk 283 3 al al NNP cord-104269-9r7rqqqk 283 4 . . NNP cord-104269-9r7rqqqk 283 5 , , , cord-104269-9r7rqqqk 283 6 1992 1992 CD cord-104269-9r7rqqqk 283 7 ; ; : cord-104269-9r7rqqqk 283 8 Halter Halter NNP cord-104269-9r7rqqqk 283 9 and and CC cord-104269-9r7rqqqk 283 10 Mellman Mellman NNP cord-104269-9r7rqqqk 283 11 , , , cord-104269-9r7rqqqk 283 12 1992 1992 CD cord-104269-9r7rqqqk 283 13 ) ) -RRB- cord-104269-9r7rqqqk 283 14 . . . cord-104269-9r7rqqqk 284 1 The the DT cord-104269-9r7rqqqk 284 2 fate fate NN cord-104269-9r7rqqqk 284 3 of of IN cord-104269-9r7rqqqk 284 4 Ii Ii NNP cord-104269-9r7rqqqk 284 5 - - HYPH cord-104269-9r7rqqqk 284 6 TR TR NNP cord-104269-9r7rqqqk 284 7 chimeras chimera NNS cord-104269-9r7rqqqk 284 8 which which WDT cord-104269-9r7rqqqk 284 9 traffic traffic VBP cord-104269-9r7rqqqk 284 10 via via IN cord-104269-9r7rqqqk 284 11 the the DT cord-104269-9r7rqqqk 284 12 plasma plasma NN cord-104269-9r7rqqqk 284 13 membrane membrane NN cord-104269-9r7rqqqk 284 14 can can MD cord-104269-9r7rqqqk 284 15 also also RB cord-104269-9r7rqqqk 284 16 be be VB cord-104269-9r7rqqqk 284 17 determined determine VBN cord-104269-9r7rqqqk 284 18 by by IN cord-104269-9r7rqqqk 284 19 loading load VBG cord-104269-9r7rqqqk 284 20 them -PRON- PRP cord-104269-9r7rqqqk 284 21 with with IN cord-104269-9r7rqqqk 284 22 exogenous exogenous JJ cord-104269-9r7rqqqk 284 23 Tf Tf NNP cord-104269-9r7rqqqk 284 24 and and CC cord-104269-9r7rqqqk 284 25 monitoring monitor VBG cord-104269-9r7rqqqk 284 26 the the DT cord-104269-9r7rqqqk 284 27 reappearance reappearance NN cord-104269-9r7rqqqk 284 28 of of IN cord-104269-9r7rqqqk 284 29 intact intact JJ cord-104269-9r7rqqqk 284 30 and and CC cord-104269-9r7rqqqk 284 31 degraded degrade VBD cord-104269-9r7rqqqk 284 32 Tf Tf NNP cord-104269-9r7rqqqk 284 33 in in IN cord-104269-9r7rqqqk 284 34 the the DT cord-104269-9r7rqqqk 284 35 medium medium NN cord-104269-9r7rqqqk 284 36 . . . cord-104269-9r7rqqqk 285 1 The the DT cord-104269-9r7rqqqk 285 2 results result NNS cord-104269-9r7rqqqk 285 3 of of IN cord-104269-9r7rqqqk 285 4 such such JJ cord-104269-9r7rqqqk 285 5 studies study NNS cord-104269-9r7rqqqk 285 6 indicated indicate VBD cord-104269-9r7rqqqk 285 7 that that IN cord-104269-9r7rqqqk 285 8 , , , cord-104269-9r7rqqqk 285 9 ~35 ~35 NFP cord-104269-9r7rqqqk 285 10 % % NN cord-104269-9r7rqqqk 285 11 of of IN cord-104269-9r7rqqqk 285 12 11CT+TM 11ct+tm CD cord-104269-9r7rqqqk 285 13 chimeras chimera NNS cord-104269-9r7rqqqk 285 14 transiently transiently RB cord-104269-9r7rqqqk 285 15 displayed display VBD cord-104269-9r7rqqqk 285 16 on on IN cord-104269-9r7rqqqk 285 17 the the DT cord-104269-9r7rqqqk 285 18 plasma plasma NN cord-104269-9r7rqqqk 285 19 membrane membrane NN cord-104269-9r7rqqqk 285 20 were be VBD cord-104269-9r7rqqqk 285 21 degraded degrade VBN cord-104269-9r7rqqqk 285 22 after after IN cord-104269-9r7rqqqk 285 23 internalization internalization NN cord-104269-9r7rqqqk 285 24 , , , cord-104269-9r7rqqqk 285 25 whereas whereas IN cord-104269-9r7rqqqk 285 26 the the DT cord-104269-9r7rqqqk 285 27 remaining remain VBG cord-104269-9r7rqqqk 285 28 , , , cord-104269-9r7rqqqk 285 29 , , , cord-104269-9r7rqqqk 285 30 065 065 CD cord-104269-9r7rqqqk 285 31 % % NN cord-104269-9r7rqqqk 285 32 were be VBD cord-104269-9r7rqqqk 285 33 recycled recycle VBN cord-104269-9r7rqqqk 285 34 back back RB cord-104269-9r7rqqqk 285 35 to to IN cord-104269-9r7rqqqk 285 36 the the DT cord-104269-9r7rqqqk 285 37 cell cell NN cord-104269-9r7rqqqk 285 38 surface surface NN cord-104269-9r7rqqqk 285 39 . . . cord-104269-9r7rqqqk 286 1 An an DT cord-104269-9r7rqqqk 286 2 even even RB cord-104269-9r7rqqqk 286 3 higher high JJR cord-104269-9r7rqqqk 286 4 fraction fraction NN cord-104269-9r7rqqqk 286 5 ( ( -LRB- cord-104269-9r7rqqqk 286 6 , , , cord-104269-9r7rqqqk 286 7 , , , cord-104269-9r7rqqqk 286 8 080 080 CD cord-104269-9r7rqqqk 286 9 % % NN cord-104269-9r7rqqqk 286 10 ) ) -RRB- cord-104269-9r7rqqqk 286 11 of of IN cord-104269-9r7rqqqk 286 12 the the DT cord-104269-9r7rqqqk 286 13 11cr 11cr JJ cord-104269-9r7rqqqk 286 14 chimera chimera NN cord-104269-9r7rqqqk 286 15 recycled recycle VBN cord-104269-9r7rqqqk 286 16 back back RB cord-104269-9r7rqqqk 286 17 to to IN cord-104269-9r7rqqqk 286 18 the the DT cord-104269-9r7rqqqk 286 19 cell cell NN cord-104269-9r7rqqqk 286 20 surface surface NN cord-104269-9r7rqqqk 286 21 . . . cord-104269-9r7rqqqk 287 1 These these DT cord-104269-9r7rqqqk 287 2 results result NNS cord-104269-9r7rqqqk 287 3 indicate indicate VBP cord-104269-9r7rqqqk 287 4 that that IN cord-104269-9r7rqqqk 287 5 the the DT cord-104269-9r7rqqqk 287 6 Ii Ii NNP cord-104269-9r7rqqqk 287 7 - - HYPH cord-104269-9r7rqqqk 287 8 TR TR NNP cord-104269-9r7rqqqk 287 9 chimeras chimera NNS cord-104269-9r7rqqqk 287 10 displayed display VBN cord-104269-9r7rqqqk 287 11 on on IN cord-104269-9r7rqqqk 287 12 the the DT cord-104269-9r7rqqqk 287 13 plasma plasma NN cord-104269-9r7rqqqk 287 14 membrane membrane NN cord-104269-9r7rqqqk 287 15 recycle recycle VB cord-104269-9r7rqqqk 287 16 several several JJ cord-104269-9r7rqqqk 287 17 times time NNS cord-104269-9r7rqqqk 287 18 before before IN cord-104269-9r7rqqqk 287 19 being be VBG cord-104269-9r7rqqqk 287 20 sorted sort VBN cord-104269-9r7rqqqk 287 21 to to IN cord-104269-9r7rqqqk 287 22 the the DT cord-104269-9r7rqqqk 287 23 endocytic endocytic JJ cord-104269-9r7rqqqk 287 24 compartment compartment NN cord-104269-9r7rqqqk 287 25 where where WRB cord-104269-9r7rqqqk 287 26 they -PRON- PRP cord-104269-9r7rqqqk 287 27 are be VBP cord-104269-9r7rqqqk 287 28 degraded degrade VBN cord-104269-9r7rqqqk 287 29 . . . cord-104269-9r7rqqqk 288 1 In in IN cord-104269-9r7rqqqk 288 2 this this DT cord-104269-9r7rqqqk 288 3 respect respect NN cord-104269-9r7rqqqk 288 4 , , , cord-104269-9r7rqqqk 288 5 11-TR 11-tr CD cord-104269-9r7rqqqk 288 6 chimeras chimera NNS cord-104269-9r7rqqqk 288 7 expressed express VBN cord-104269-9r7rqqqk 288 8 on on IN cord-104269-9r7rqqqk 288 9 the the DT cord-104269-9r7rqqqk 288 10 cell cell NN cord-104269-9r7rqqqk 288 11 surface surface NN cord-104269-9r7rqqqk 288 12 behave behave VBP cord-104269-9r7rqqqk 288 13 similarly similarly RB cord-104269-9r7rqqqk 288 14 to to IN cord-104269-9r7rqqqk 288 15 lysosomal lysosomal JJ cord-104269-9r7rqqqk 288 16 acid acid NN cord-104269-9r7rqqqk 288 17 phosphatase phosphatase NN cord-104269-9r7rqqqk 288 18 which which WDT cord-104269-9r7rqqqk 288 19 is be VBZ cord-104269-9r7rqqqk 288 20 transported transport VBN cord-104269-9r7rqqqk 288 21 to to IN cord-104269-9r7rqqqk 288 22 the the DT cord-104269-9r7rqqqk 288 23 cell cell NN cord-104269-9r7rqqqk 288 24 surface surface NN cord-104269-9r7rqqqk 288 25 and and CC cord-104269-9r7rqqqk 288 26 undergoes undergo VBZ cord-104269-9r7rqqqk 288 27 multiple multiple JJ cord-104269-9r7rqqqk 288 28 rounds round NNS cord-104269-9r7rqqqk 288 29 of of IN cord-104269-9r7rqqqk 288 30 internalization internalization NN cord-104269-9r7rqqqk 288 31 and and CC cord-104269-9r7rqqqk 288 32 recycling recycling NN cord-104269-9r7rqqqk 288 33 before before IN cord-104269-9r7rqqqk 288 34 being be VBG cord-104269-9r7rqqqk 288 35 transferred transfer VBN cord-104269-9r7rqqqk 288 36 to to IN cord-104269-9r7rqqqk 288 37 the the DT cord-104269-9r7rqqqk 288 38 lysosome lysosome NN cord-104269-9r7rqqqk 288 39 ( ( -LRB- cord-104269-9r7rqqqk 288 40 Braun Braun NNP cord-104269-9r7rqqqk 288 41 et et NNP cord-104269-9r7rqqqk 288 42 al al NNP cord-104269-9r7rqqqk 288 43 . . NNP cord-104269-9r7rqqqk 288 44 , , , cord-104269-9r7rqqqk 288 45 1989 1989 CD cord-104269-9r7rqqqk 288 46 ) ) -RRB- cord-104269-9r7rqqqk 288 47 . . . cord-104269-9r7rqqqk 289 1 Studies study NNS cord-104269-9r7rqqqk 289 2 on on IN cord-104269-9r7rqqqk 289 3 the the DT cord-104269-9r7rqqqk 289 4 trafficking trafficking NN cord-104269-9r7rqqqk 289 5 of of IN cord-104269-9r7rqqqk 289 6 the the DT cord-104269-9r7rqqqk 289 7 cation cation NN cord-104269-9r7rqqqk 289 8 - - HYPH cord-104269-9r7rqqqk 289 9 independent independent JJ cord-104269-9r7rqqqk 289 10 mannose-6-phosphate mannose-6-phosphate NNP cord-104269-9r7rqqqk 289 11 receptor receptor NN cord-104269-9r7rqqqk 289 12 ( ( -LRB- cord-104269-9r7rqqqk 289 13 M6PR M6PR NNP cord-104269-9r7rqqqk 289 14 ) ) -RRB- cord-104269-9r7rqqqk 289 15 have have VBP cord-104269-9r7rqqqk 289 16 clearly clearly RB cord-104269-9r7rqqqk 289 17 established establish VBN cord-104269-9r7rqqqk 289 18 that that IN cord-104269-9r7rqqqk 289 19 a a DT cord-104269-9r7rqqqk 289 20 direct direct JJ cord-104269-9r7rqqqk 289 21 route route NN cord-104269-9r7rqqqk 289 22 from from IN cord-104269-9r7rqqqk 289 23 the the DT cord-104269-9r7rqqqk 289 24 trans trans NNP cord-104269-9r7rqqqk 289 25 - - NNP cord-104269-9r7rqqqk 289 26 Golgi Golgi NNP cord-104269-9r7rqqqk 289 27 to to IN cord-104269-9r7rqqqk 289 28 the the DT cord-104269-9r7rqqqk 289 29 endocytic endocytic JJ cord-104269-9r7rqqqk 289 30 pathway pathway NN cord-104269-9r7rqqqk 289 31 exists exist VBZ cord-104269-9r7rqqqk 289 32 ( ( -LRB- cord-104269-9r7rqqqk 289 33 Kornfield Kornfield NNP cord-104269-9r7rqqqk 289 34 and and CC cord-104269-9r7rqqqk 289 35 Mellman Mellman NNP cord-104269-9r7rqqqk 289 36 1989 1989 CD cord-104269-9r7rqqqk 289 37 ) ) -RRB- cord-104269-9r7rqqqk 289 38 . . . cord-104269-9r7rqqqk 290 1 It -PRON- PRP cord-104269-9r7rqqqk 290 2 has have VBZ cord-104269-9r7rqqqk 290 3 been be VBN cord-104269-9r7rqqqk 290 4 shown show VBN cord-104269-9r7rqqqk 290 5 that that IN cord-104269-9r7rqqqk 290 6 M6PR M6PR NNP cord-104269-9r7rqqqk 290 7 are be VBP cord-104269-9r7rqqqk 290 8 concentrated concentrate VBN cord-104269-9r7rqqqk 290 9 within within IN cord-104269-9r7rqqqk 290 10 clathrin clathrin NN cord-104269-9r7rqqqk 290 11 - - HYPH cord-104269-9r7rqqqk 290 12 coated coat VBN cord-104269-9r7rqqqk 290 13 buds bud NNS cord-104269-9r7rqqqk 290 14 in in IN cord-104269-9r7rqqqk 290 15 the the DT cord-104269-9r7rqqqk 290 16 trans trans NNP cord-104269-9r7rqqqk 290 17 - - NNP cord-104269-9r7rqqqk 290 18 Golgi Golgi NNP cord-104269-9r7rqqqk 291 1 ( ( -LRB- cord-104269-9r7rqqqk 291 2 Rijnboutt Rijnboutt NNP cord-104269-9r7rqqqk 291 3 et et NNP cord-104269-9r7rqqqk 291 4 al al NNP cord-104269-9r7rqqqk 291 5 . . . cord-104269-9r7rqqqk 292 1 , , , cord-104269-9r7rqqqk 292 2 1992 1992 CD cord-104269-9r7rqqqk 292 3 ; ; : cord-104269-9r7rqqqk 292 4 Klumperman Klumperman NNP cord-104269-9r7rqqqk 292 5 et et FW cord-104269-9r7rqqqk 292 6 al al NNP cord-104269-9r7rqqqk 292 7 . . NNP cord-104269-9r7rqqqk 292 8 , , , cord-104269-9r7rqqqk 292 9 1993 1993 CD cord-104269-9r7rqqqk 292 10 ) ) -RRB- cord-104269-9r7rqqqk 292 11 and and CC cord-104269-9r7rqqqk 292 12 there there EX cord-104269-9r7rqqqk 292 13 is be VBZ cord-104269-9r7rqqqk 292 14 evidence evidence NN cord-104269-9r7rqqqk 292 15 suggesting suggest VBG cord-104269-9r7rqqqk 292 16 that that IN cord-104269-9r7rqqqk 292 17 they -PRON- PRP cord-104269-9r7rqqqk 292 18 are be VBP cord-104269-9r7rqqqk 292 19 then then RB cord-104269-9r7rqqqk 292 20 delivered deliver VBN cord-104269-9r7rqqqk 292 21 to to IN cord-104269-9r7rqqqk 292 22 an an DT cord-104269-9r7rqqqk 292 23 early early JJ cord-104269-9r7rqqqk 292 24 part part NN cord-104269-9r7rqqqk 292 25 of of IN cord-104269-9r7rqqqk 292 26 the the DT cord-104269-9r7rqqqk 292 27 endocytic endocytic JJ cord-104269-9r7rqqqk 292 28 pathway pathway NN cord-104269-9r7rqqqk 292 29 , , , cord-104269-9r7rqqqk 292 30 most most RBS cord-104269-9r7rqqqk 292 31 probably probably RB cord-104269-9r7rqqqk 292 32 the the DT cord-104269-9r7rqqqk 292 33 TR tr NN cord-104269-9r7rqqqk 292 34 - - HYPH cord-104269-9r7rqqqk 292 35 containing contain VBG cord-104269-9r7rqqqk 292 36 endosome endosome NN cord-104269-9r7rqqqk 292 37 ( ( -LRB- cord-104269-9r7rqqqk 292 38 Ludwig Ludwig NNP cord-104269-9r7rqqqk 292 39 et et NNP cord-104269-9r7rqqqk 292 40 al al NNP cord-104269-9r7rqqqk 292 41 . . NNP cord-104269-9r7rqqqk 292 42 , , , cord-104269-9r7rqqqk 292 43 1991 1991 CD cord-104269-9r7rqqqk 292 44 ; ; : cord-104269-9r7rqqqk 292 45 M6resse M6resse NNP cord-104269-9r7rqqqk 292 46 and and CC cord-104269-9r7rqqqk 292 47 Hoflack Hoflack NNP cord-104269-9r7rqqqk 292 48 , , , cord-104269-9r7rqqqk 292 49 1993 1993 CD cord-104269-9r7rqqqk 292 50 ) ) -RRB- cord-104269-9r7rqqqk 292 51 . . . cord-104269-9r7rqqqk 293 1 Previous previous JJ cord-104269-9r7rqqqk 293 2 work work NN cord-104269-9r7rqqqk 293 3 on on IN cord-104269-9r7rqqqk 293 4 Ii Ii NNP cord-104269-9r7rqqqk 293 5 ( ( -LRB- cord-104269-9r7rqqqk 293 6 Guagliardi Guagliardi NNP cord-104269-9r7rqqqk 293 7 et et NNP cord-104269-9r7rqqqk 293 8 al al NNP cord-104269-9r7rqqqk 293 9 . . NNP cord-104269-9r7rqqqk 293 10 , , , cord-104269-9r7rqqqk 293 11 1990 1990 CD cord-104269-9r7rqqqk 293 12 ; ; : cord-104269-9r7rqqqk 293 13 Pieters Pieters NNP cord-104269-9r7rqqqk 293 14 et et NNP cord-104269-9r7rqqqk 293 15 al al NNP cord-104269-9r7rqqqk 293 16 . . NNP cord-104269-9r7rqqqk 293 17 , , , cord-104269-9r7rqqqk 293 18 1991 1991 CD cord-104269-9r7rqqqk 293 19 ; ; : cord-104269-9r7rqqqk 293 20 Zachgo Zachgo NNP cord-104269-9r7rqqqk 293 21 et et NNP cord-104269-9r7rqqqk 293 22 al al NNP cord-104269-9r7rqqqk 293 23 . . . cord-104269-9r7rqqqk 294 1 , , , cord-104269-9r7rqqqk 294 2 1992 1992 CD cord-104269-9r7rqqqk 294 3 ; ; : cord-104269-9r7rqqqk 294 4 Romagnoli Romagnoli NNP cord-104269-9r7rqqqk 294 5 et et FW cord-104269-9r7rqqqk 294 6 al al NNP cord-104269-9r7rqqqk 294 7 . . NNP cord-104269-9r7rqqqk 294 8 , , , cord-104269-9r7rqqqk 294 9 1993 1993 CD cord-104269-9r7rqqqk 294 10 ) ) -RRB- cord-104269-9r7rqqqk 294 11 also also RB cord-104269-9r7rqqqk 294 12 suggest suggest VBP cord-104269-9r7rqqqk 294 13 that that IN cord-104269-9r7rqqqk 294 14 entry entry NN cord-104269-9r7rqqqk 294 15 into into IN cord-104269-9r7rqqqk 294 16 the the DT cord-104269-9r7rqqqk 294 17 endocytic endocytic JJ cord-104269-9r7rqqqk 294 18 pathway pathway NN cord-104269-9r7rqqqk 294 19 is be VBZ cord-104269-9r7rqqqk 294 20 via via IN cord-104269-9r7rqqqk 294 21 the the DT cord-104269-9r7rqqqk 294 22 TR tr NN cord-104269-9r7rqqqk 294 23 - - HYPH cord-104269-9r7rqqqk 294 24 containing contain VBG cord-104269-9r7rqqqk 294 25 endosome endosome NN cord-104269-9r7rqqqk 294 26 . . . cord-104269-9r7rqqqk 295 1 Our -PRON- PRP$ cord-104269-9r7rqqqk 295 2 data datum NNS cord-104269-9r7rqqqk 295 3 show show VBP cord-104269-9r7rqqqk 295 4 that that IN cord-104269-9r7rqqqk 295 5 the the DT cord-104269-9r7rqqqk 295 6 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 295 7 domain domain NN cord-104269-9r7rqqqk 295 8 of of IN cord-104269-9r7rqqqk 295 9 Ii Ii NNP cord-104269-9r7rqqqk 295 10 contains contain VBZ cord-104269-9r7rqqqk 295 11 two two CD cord-104269-9r7rqqqk 295 12 signals signal NNS cord-104269-9r7rqqqk 295 13 recognized recognize VBN cord-104269-9r7rqqqk 295 14 in in IN cord-104269-9r7rqqqk 295 15 the the DT cord-104269-9r7rqqqk 295 16 trans trans NNP cord-104269-9r7rqqqk 295 17 - - NNP cord-104269-9r7rqqqk 295 18 Golgi Golgi NNP cord-104269-9r7rqqqk 295 19 that that WDT cord-104269-9r7rqqqk 295 20 are be VBP cord-104269-9r7rqqqk 295 21 the the DT cord-104269-9r7rqqqk 295 22 same same JJ cord-104269-9r7rqqqk 295 23 or or CC cord-104269-9r7rqqqk 295 24 overlap overlap VB cord-104269-9r7rqqqk 295 25 with with IN cord-104269-9r7rqqqk 295 26 the the DT cord-104269-9r7rqqqk 295 27 internalization internalization NN cord-104269-9r7rqqqk 295 28 signals signal NNS cord-104269-9r7rqqqk 295 29 recognized recognize VBN cord-104269-9r7rqqqk 295 30 by by IN cord-104269-9r7rqqqk 295 31 clathrin clathrin NN cord-104269-9r7rqqqk 295 32 - - HYPH cord-104269-9r7rqqqk 295 33 coated coat VBN cord-104269-9r7rqqqk 295 34 domains domain NNS cord-104269-9r7rqqqk 295 35 at at IN cord-104269-9r7rqqqk 295 36 the the DT cord-104269-9r7rqqqk 295 37 plasma plasma NN cord-104269-9r7rqqqk 295 38 membrane membrane NN cord-104269-9r7rqqqk 295 39 . . . cord-104269-9r7rqqqk 296 1 This this DT cord-104269-9r7rqqqk 296 2 relationship relationship NN cord-104269-9r7rqqqk 296 3 implies imply VBZ cord-104269-9r7rqqqk 296 4 that that IN cord-104269-9r7rqqqk 296 5 the the DT cord-104269-9r7rqqqk 296 6 signals signal NNS cord-104269-9r7rqqqk 296 7 active active JJ cord-104269-9r7rqqqk 296 8 in in IN cord-104269-9r7rqqqk 296 9 the the DT cord-104269-9r7rqqqk 296 10 trans trans NNP cord-104269-9r7rqqqk 296 11 - - NNP cord-104269-9r7rqqqk 296 12 Golgi Golgi NNP cord-104269-9r7rqqqk 296 13 are be VBP cord-104269-9r7rqqqk 296 14 recognized recognize VBN cord-104269-9r7rqqqk 296 15 by by IN cord-104269-9r7rqqqk 296 16 clathrin clathrin NN cord-104269-9r7rqqqk 296 17 - - HYPH cord-104269-9r7rqqqk 296 18 based base VBN cord-104269-9r7rqqqk 296 19 sorting sorting NN cord-104269-9r7rqqqk 296 20 machinery machinery NN cord-104269-9r7rqqqk 296 21 . . . cord-104269-9r7rqqqk 297 1 It -PRON- PRP cord-104269-9r7rqqqk 297 2 is be VBZ cord-104269-9r7rqqqk 297 3 also also RB cord-104269-9r7rqqqk 297 4 apparent apparent JJ cord-104269-9r7rqqqk 297 5 , , , cord-104269-9r7rqqqk 297 6 however however RB cord-104269-9r7rqqqk 297 7 , , , cord-104269-9r7rqqqk 297 8 that that IN cord-104269-9r7rqqqk 297 9 for for IN cord-104269-9r7rqqqk 297 10 direct direct JJ cord-104269-9r7rqqqk 297 11 transfer transfer NN cord-104269-9r7rqqqk 297 12 of of IN cord-104269-9r7rqqqk 297 13 Ii Ii NNP cord-104269-9r7rqqqk 297 14 from from IN cord-104269-9r7rqqqk 297 15 the the DT cord-104269-9r7rqqqk 297 16 trans trans NNP cord-104269-9r7rqqqk 297 17 - - NNP cord-104269-9r7rqqqk 297 18 Golgi Golgi NNP cord-104269-9r7rqqqk 297 19 to to IN cord-104269-9r7rqqqk 297 20 the the DT cord-104269-9r7rqqqk 297 21 endocytic endocytic JJ cord-104269-9r7rqqqk 297 22 pathway pathway NN cord-104269-9r7rqqqk 297 23 to to TO cord-104269-9r7rqqqk 297 24 operate operate VB cord-104269-9r7rqqqk 297 25 with with IN cord-104269-9r7rqqqk 297 26 full full JJ cord-104269-9r7rqqqk 297 27 efficiency efficiency NN cord-104269-9r7rqqqk 297 28 , , , cord-104269-9r7rqqqk 297 29 additional additional JJ cord-104269-9r7rqqqk 297 30 signal signal NN cord-104269-9r7rqqqk 297 31 information information NN cord-104269-9r7rqqqk 297 32 within within IN cord-104269-9r7rqqqk 297 33 the the DT cord-104269-9r7rqqqk 297 34 transmembrane transmembrane NNP cord-104269-9r7rqqqk 297 35 region region NN cord-104269-9r7rqqqk 297 36 is be VBZ cord-104269-9r7rqqqk 297 37 required require VBN cord-104269-9r7rqqqk 297 38 . . . cord-104269-9r7rqqqk 298 1 The the DT cord-104269-9r7rqqqk 298 2 Ii Ii NNP cord-104269-9r7rqqqk 298 3 transmembrane transmembrane NNP cord-104269-9r7rqqqk 298 4 region region NN cord-104269-9r7rqqqk 298 5 also also RB cord-104269-9r7rqqqk 298 6 increases increase VBZ cord-104269-9r7rqqqk 298 7 the the DT cord-104269-9r7rqqqk 298 8 efficiency efficiency NN cord-104269-9r7rqqqk 298 9 of of IN cord-104269-9r7rqqqk 298 10 trafficking traffic VBG cord-104269-9r7rqqqk 298 11 from from IN cord-104269-9r7rqqqk 298 12 the the DT cord-104269-9r7rqqqk 298 13 plasma plasma NN cord-104269-9r7rqqqk 298 14 membrane membrane NN cord-104269-9r7rqqqk 298 15 to to IN cord-104269-9r7rqqqk 298 16 the the DT cord-104269-9r7rqqqk 298 17 degradative degradative NNP cord-104269-9r7rqqqk 298 18 endocytic endocytic NNP cord-104269-9r7rqqqk 298 19 compartment compartment NN cord-104269-9r7rqqqk 298 20 . . . cord-104269-9r7rqqqk 299 1 Golgi Golgi NNP cord-104269-9r7rqqqk 299 2 retention retention NN cord-104269-9r7rqqqk 299 3 signals signal NNS cord-104269-9r7rqqqk 299 4 have have VBP cord-104269-9r7rqqqk 299 5 previously previously RB cord-104269-9r7rqqqk 299 6 been be VBN cord-104269-9r7rqqqk 299 7 localized localize VBN cord-104269-9r7rqqqk 299 8 to to IN cord-104269-9r7rqqqk 299 9 the the DT cord-104269-9r7rqqqk 299 10 transmembrane transmembrane NN cord-104269-9r7rqqqk 299 11 region region NN cord-104269-9r7rqqqk 299 12 ( ( -LRB- cord-104269-9r7rqqqk 299 13 Machamer Machamer NNP cord-104269-9r7rqqqk 299 14 and and CC cord-104269-9r7rqqqk 299 15 Rose Rose NNP cord-104269-9r7rqqqk 299 16 , , , cord-104269-9r7rqqqk 299 17 1987 1987 CD cord-104269-9r7rqqqk 299 18 ; ; : cord-104269-9r7rqqqk 299 19 Swift Swift NNP cord-104269-9r7rqqqk 299 20 and and CC cord-104269-9r7rqqqk 299 21 Machamer Machamer NNP cord-104269-9r7rqqqk 299 22 , , , cord-104269-9r7rqqqk 299 23 1991 1991 CD cord-104269-9r7rqqqk 299 24 ; ; : cord-104269-9r7rqqqk 299 25 Munro Munro NNP cord-104269-9r7rqqqk 299 26 , , , cord-104269-9r7rqqqk 299 27 1991 1991 CD cord-104269-9r7rqqqk 299 28 ; ; : cord-104269-9r7rqqqk 299 29 Nilsson Nilsson NNP cord-104269-9r7rqqqk 299 30 et et NNP cord-104269-9r7rqqqk 299 31 al al NNP cord-104269-9r7rqqqk 299 32 . . NNP cord-104269-9r7rqqqk 299 33 , , , cord-104269-9r7rqqqk 299 34 1991 1991 CD cord-104269-9r7rqqqk 299 35 ; ; : cord-104269-9r7rqqqk 299 36 Wong Wong NNP cord-104269-9r7rqqqk 299 37 et et NNP cord-104269-9r7rqqqk 299 38 al al NNP cord-104269-9r7rqqqk 299 39 . . NNP cord-104269-9r7rqqqk 299 40 , , , cord-104269-9r7rqqqk 299 41 1992 1992 CD cord-104269-9r7rqqqk 299 42 ) ) -RRB- cord-104269-9r7rqqqk 299 43 . . . cord-104269-9r7rqqqk 300 1 However however RB cord-104269-9r7rqqqk 300 2 , , , cord-104269-9r7rqqqk 300 3 there there EX cord-104269-9r7rqqqk 300 4 is be VBZ cord-104269-9r7rqqqk 300 5 no no DT cord-104269-9r7rqqqk 300 6 precedent precedent NN cord-104269-9r7rqqqk 300 7 for for IN cord-104269-9r7rqqqk 300 8 the the DT cord-104269-9r7rqqqk 300 9 transmembrane transmembrane NNP cord-104269-9r7rqqqk 300 10 region region NN cord-104269-9r7rqqqk 300 11 of of IN cord-104269-9r7rqqqk 300 12 an an DT cord-104269-9r7rqqqk 300 13 integral integral JJ cord-104269-9r7rqqqk 300 14 membrane membrane NN cord-104269-9r7rqqqk 300 15 protein protein NN cord-104269-9r7rqqqk 300 16 influencing influence VBG cord-104269-9r7rqqqk 300 17 sorting sorting NN cord-104269-9r7rqqqk 300 18 in in IN cord-104269-9r7rqqqk 300 19 the the DT cord-104269-9r7rqqqk 300 20 trans trans NNP cord-104269-9r7rqqqk 300 21 - - NNP cord-104269-9r7rqqqk 300 22 Golgi Golgi NNP cord-104269-9r7rqqqk 300 23 or or CC cord-104269-9r7rqqqk 300 24 trafficking trafficking NN cord-104269-9r7rqqqk 300 25 along along IN cord-104269-9r7rqqqk 300 26 the the DT cord-104269-9r7rqqqk 300 27 endocytic endocytic JJ cord-104269-9r7rqqqk 300 28 pathway pathway NN cord-104269-9r7rqqqk 300 29 . . . cord-104269-9r7rqqqk 301 1 Our -PRON- PRP$ cord-104269-9r7rqqqk 301 2 evidence evidence NN cord-104269-9r7rqqqk 301 3 shows show VBZ cord-104269-9r7rqqqk 301 4 that that IN cord-104269-9r7rqqqk 301 5 Ii Ii NNP cord-104269-9r7rqqqk 301 6 , , , cord-104269-9r7rqqqk 301 7 like like IN cord-104269-9r7rqqqk 301 8 the the DT cord-104269-9r7rqqqk 301 9 M6PR m6pr NN cord-104269-9r7rqqqk 302 1 ( ( -LRB- cord-104269-9r7rqqqk 302 2 Kornfield Kornfield NNP cord-104269-9r7rqqqk 302 3 and and CC cord-104269-9r7rqqqk 302 4 Mellman Mellman NNP cord-104269-9r7rqqqk 302 5 , , , cord-104269-9r7rqqqk 302 6 1989 1989 CD cord-104269-9r7rqqqk 302 7 ) ) -RRB- cord-104269-9r7rqqqk 302 8 , , , cord-104269-9r7rqqqk 302 9 enters enter VBZ cord-104269-9r7rqqqk 302 10 the the DT cord-104269-9r7rqqqk 302 11 TR tr NN cord-104269-9r7rqqqk 302 12 - - HYPH cord-104269-9r7rqqqk 302 13 containing contain VBG cord-104269-9r7rqqqk 302 14 endosome endosome NN cord-104269-9r7rqqqk 302 15 from from IN cord-104269-9r7rqqqk 302 16 both both CC cord-104269-9r7rqqqk 302 17 the the DT cord-104269-9r7rqqqk 302 18 trans trans NNP cord-104269-9r7rqqqk 302 19 - - NNP cord-104269-9r7rqqqk 302 20 Golgi Golgi NNP cord-104269-9r7rqqqk 302 21 and and CC cord-104269-9r7rqqqk 302 22 the the DT cord-104269-9r7rqqqk 302 23 plasma plasma NN cord-104269-9r7rqqqk 302 24 membrane membrane NN cord-104269-9r7rqqqk 302 25 . . . cord-104269-9r7rqqqk 303 1 However however RB cord-104269-9r7rqqqk 303 2 , , , cord-104269-9r7rqqqk 303 3 the the DT cord-104269-9r7rqqqk 303 4 data datum NNS cord-104269-9r7rqqqk 303 5 also also RB cord-104269-9r7rqqqk 303 6 indicate indicate VBP cord-104269-9r7rqqqk 303 7 that that IN cord-104269-9r7rqqqk 303 8 the the DT cord-104269-9r7rqqqk 303 9 Ii Ii NNP cord-104269-9r7rqqqk 303 10 - - HYPH cord-104269-9r7rqqqk 303 11 TR TR NNP cord-104269-9r7rqqqk 303 12 chimeras chimera NNS cord-104269-9r7rqqqk 303 13 which which WDT cord-104269-9r7rqqqk 303 14 enter enter VBP cord-104269-9r7rqqqk 303 15 directly directly RB cord-104269-9r7rqqqk 303 16 from from IN cord-104269-9r7rqqqk 303 17 the the DT cord-104269-9r7rqqqk 303 18 trans trans NNP cord-104269-9r7rqqqk 303 19 - - NNP cord-104269-9r7rqqqk 303 20 Golgi Golgi NNP cord-104269-9r7rqqqk 303 21 are be VBP cord-104269-9r7rqqqk 303 22 delivered deliver VBN cord-104269-9r7rqqqk 303 23 to to IN cord-104269-9r7rqqqk 303 24 a a DT cord-104269-9r7rqqqk 303 25 degradafive degradafive JJ cord-104269-9r7rqqqk 303 26 compartment compartment NN cord-104269-9r7rqqqk 303 27 more more RBR cord-104269-9r7rqqqk 303 28 efficiently efficiently RB cord-104269-9r7rqqqk 303 29 than than IN cord-104269-9r7rqqqk 303 30 those those DT cord-104269-9r7rqqqk 303 31 which which WDT cord-104269-9r7rqqqk 303 32 enter enter VBP cord-104269-9r7rqqqk 303 33 from from IN cord-104269-9r7rqqqk 303 34 the the DT cord-104269-9r7rqqqk 303 35 plasma plasma NN cord-104269-9r7rqqqk 303 36 membrane membrane NN cord-104269-9r7rqqqk 303 37 . . . cord-104269-9r7rqqqk 304 1 Our -PRON- PRP$ cord-104269-9r7rqqqk 304 2 recent recent JJ cord-104269-9r7rqqqk 304 3 observations observation NNS cord-104269-9r7rqqqk 304 4 on on IN cord-104269-9r7rqqqk 304 5 the the DT cord-104269-9r7rqqqk 304 6 TR tr NN cord-104269-9r7rqqqk 304 7 - - HYPH cord-104269-9r7rqqqk 304 8 contalning contalne VBG cord-104269-9r7rqqqk 304 9 endosome endosome NN cord-104269-9r7rqqqk 304 10 in in IN cord-104269-9r7rqqqk 304 11 CEF CEF NNP cord-104269-9r7rqqqk 305 1 ( ( -LRB- cord-104269-9r7rqqqk 305 2 Hopkins Hopkins NNP cord-104269-9r7rqqqk 305 3 et et NNP cord-104269-9r7rqqqk 305 4 al al NNP cord-104269-9r7rqqqk 305 5 . . NNP cord-104269-9r7rqqqk 305 6 , , , cord-104269-9r7rqqqk 305 7 1994 1994 CD cord-104269-9r7rqqqk 305 8 ) ) -RRB- cord-104269-9r7rqqqk 305 9 show show VBP cord-104269-9r7rqqqk 305 10 that that IN cord-104269-9r7rqqqk 305 11 it -PRON- PRP cord-104269-9r7rqqqk 305 12 is be VBZ cord-104269-9r7rqqqk 305 13 a a DT cord-104269-9r7rqqqk 305 14 morphologically morphologically RB cord-104269-9r7rqqqk 305 15 complex complex JJ cord-104269-9r7rqqqk 305 16 compatmient compatmient NN cord-104269-9r7rqqqk 305 17 and and CC cord-104269-9r7rqqqk 305 18 suggest suggest VBP cord-104269-9r7rqqqk 305 19 that that IN cord-104269-9r7rqqqk 305 20 it -PRON- PRP cord-104269-9r7rqqqk 305 21 consists consist VBZ cord-104269-9r7rqqqk 305 22 of of IN cord-104269-9r7rqqqk 305 23 at at RB cord-104269-9r7rqqqk 305 24 least least RBS cord-104269-9r7rqqqk 305 25 two two CD cord-104269-9r7rqqqk 305 26 interconnected interconnected JJ cord-104269-9r7rqqqk 305 27 subcompartments subcompartment NNS cord-104269-9r7rqqqk 305 28 in in IN cord-104269-9r7rqqqk 305 29 which which WDT cord-104269-9r7rqqqk 305 30 internalized internalize VBN cord-104269-9r7rqqqk 305 31 TRs tr NNS cord-104269-9r7rqqqk 305 32 are be VBP cord-104269-9r7rqqqk 305 33 processed process VBN cord-104269-9r7rqqqk 305 34 at at IN cord-104269-9r7rqqqk 305 35 different different JJ cord-104269-9r7rqqqk 305 36 rates rate NNS cord-104269-9r7rqqqk 305 37 . . . cord-104269-9r7rqqqk 306 1 Receptors receptor NNS cord-104269-9r7rqqqk 306 2 entering enter VBG cord-104269-9r7rqqqk 306 3 the the DT cord-104269-9r7rqqqk 306 4 TR tr NN cord-104269-9r7rqqqk 306 5 - - HYPH cord-104269-9r7rqqqk 306 6 containing contain VBG cord-104269-9r7rqqqk 306 7 endosome endosome NN cord-104269-9r7rqqqk 306 8 can can MD cord-104269-9r7rqqqk 306 9 either either CC cord-104269-9r7rqqqk 306 10 be be VB cord-104269-9r7rqqqk 306 11 recycled recycle VBN cord-104269-9r7rqqqk 306 12 rapidly rapidly RB cord-104269-9r7rqqqk 306 13 ( ( -LRB- cord-104269-9r7rqqqk 306 14 tee tee NN cord-104269-9r7rqqqk 306 15 in in IN cord-104269-9r7rqqqk 306 16 10 10 CD cord-104269-9r7rqqqk 306 17 rain rain NN cord-104269-9r7rqqqk 306 18 ) ) -RRB- cord-104269-9r7rqqqk 306 19 or or CC cord-104269-9r7rqqqk 306 20 remain remain VB cord-104269-9r7rqqqk 306 21 for for IN cord-104269-9r7rqqqk 306 22 more more RBR cord-104269-9r7rqqqk 306 23 prolonged prolong VBN cord-104269-9r7rqqqk 306 24 periods period NNS cord-104269-9r7rqqqk 306 25 ( ( -LRB- cord-104269-9r7rqqqk 306 26 t t NN cord-104269-9r7rqqqk 306 27 ~ ~ CD cord-104269-9r7rqqqk 306 28 r2 r2 NNP cord-104269-9r7rqqqk 306 29 in in IN cord-104269-9r7rqqqk 306 30 30 30 CD cord-104269-9r7rqqqk 306 31 min min NN cord-104269-9r7rqqqk 306 32 ) ) -RRB- cord-104269-9r7rqqqk 306 33 before before IN cord-104269-9r7rqqqk 306 34 returning return VBG cord-104269-9r7rqqqk 306 35 to to IN cord-104269-9r7rqqqk 306 36 the the DT cord-104269-9r7rqqqk 306 37 surface surface NN cord-104269-9r7rqqqk 306 38 . . . cord-104269-9r7rqqqk 307 1 The the DT cord-104269-9r7rqqqk 307 2 data datum NNS cord-104269-9r7rqqqk 307 3 on on IN cord-104269-9r7rqqqk 307 4 Ii Ii NNP cord-104269-9r7rqqqk 307 5 - - HYPH cord-104269-9r7rqqqk 307 6 TR TR NNP cord-104269-9r7rqqqk 307 7 chimeras chimera NNS cord-104269-9r7rqqqk 307 8 can can MD cord-104269-9r7rqqqk 307 9 thus thus RB cord-104269-9r7rqqqk 307 10 be be VB cord-104269-9r7rqqqk 307 11 explained explain VBN cord-104269-9r7rqqqk 307 12 by by IN cord-104269-9r7rqqqk 307 13 proposing propose VBG cord-104269-9r7rqqqk 307 14 that that IN cord-104269-9r7rqqqk 307 15 most most JJS cord-104269-9r7rqqqk 307 16 of of IN cord-104269-9r7rqqqk 307 17 the the DT cord-104269-9r7rqqqk 307 18 chimeras chimera NNS cord-104269-9r7rqqqk 307 19 which which WDT cord-104269-9r7rqqqk 307 20 enter enter VBP cord-104269-9r7rqqqk 307 21 from from IN cord-104269-9r7rqqqk 307 22 the the DT cord-104269-9r7rqqqk 307 23 cell cell NN cord-104269-9r7rqqqk 307 24 surface surface NN cord-104269-9r7rqqqk 307 25 travel travel NN cord-104269-9r7rqqqk 307 26 via via IN cord-104269-9r7rqqqk 307 27 the the DT cord-104269-9r7rqqqk 307 28 rapidly rapidly RB cord-104269-9r7rqqqk 307 29 recycling recycle VBG cord-104269-9r7rqqqk 307 30 pathway pathway NN cord-104269-9r7rqqqk 307 31 and and CC cord-104269-9r7rqqqk 307 32 that that IN cord-104269-9r7rqqqk 307 33 on on IN cord-104269-9r7rqqqk 307 34 each each DT cord-104269-9r7rqqqk 307 35 cycle cycle NN cord-104269-9r7rqqqk 307 36 only only RB cord-104269-9r7rqqqk 307 37 •30 •30 : cord-104269-9r7rqqqk 307 38 % % NN cord-104269-9r7rqqqk 307 39 of of IN cord-104269-9r7rqqqk 307 40 them -PRON- PRP cord-104269-9r7rqqqk 307 41 are be VBP cord-104269-9r7rqqqk 307 42 sorted sort VBN cord-104269-9r7rqqqk 307 43 towards towards IN cord-104269-9r7rqqqk 307 44 the the DT cord-104269-9r7rqqqk 307 45 lysosome lysosome NN cord-104269-9r7rqqqk 307 46 . . . cord-104269-9r7rqqqk 308 1 Direct direct JJ cord-104269-9r7rqqqk 308 2 transfer transfer NN cord-104269-9r7rqqqk 308 3 from from IN cord-104269-9r7rqqqk 308 4 the the DT cord-104269-9r7rqqqk 308 5 trans trans NNP cord-104269-9r7rqqqk 308 6 - - NNP cord-104269-9r7rqqqk 308 7 Golgi Golgi NNP cord-104269-9r7rqqqk 308 8 , , , cord-104269-9r7rqqqk 308 9 though though IN cord-104269-9r7rqqqk 308 10 more more RBR cord-104269-9r7rqqqk 308 11 efficient efficient JJ cord-104269-9r7rqqqk 308 12 , , , cord-104269-9r7rqqqk 308 13 probably probably RB cord-104269-9r7rqqqk 308 14 occurs occur VBZ cord-104269-9r7rqqqk 308 15 via via IN cord-104269-9r7rqqqk 308 16 the the DT cord-104269-9r7rqqqk 308 17 subcompartment subcompartment NN cord-104269-9r7rqqqk 308 18 of of IN cord-104269-9r7rqqqk 308 19 the the DT cord-104269-9r7rqqqk 308 20 endosome endosome NN cord-104269-9r7rqqqk 308 21 which which WDT cord-104269-9r7rqqqk 308 22 lies lie VBZ cord-104269-9r7rqqqk 308 23 within within IN cord-104269-9r7rqqqk 308 24 its -PRON- PRP$ cord-104269-9r7rqqqk 308 25 immediate immediate JJ cord-104269-9r7rqqqk 308 26 vicinity vicinity NN cord-104269-9r7rqqqk 308 27 in in IN cord-104269-9r7rqqqk 308 28 the the DT cord-104269-9r7rqqqk 308 29 pericentriolar pericentriolar NNP cord-104269-9r7rqqqk 308 30 area area NN cord-104269-9r7rqqqk 308 31 and and CC cord-104269-9r7rqqqk 308 32 through through IN cord-104269-9r7rqqqk 308 33 which which WDT cord-104269-9r7rqqqk 308 34 TR tr NN cord-104269-9r7rqqqk 308 35 are be VBP cord-104269-9r7rqqqk 308 36 processed process VBN cord-104269-9r7rqqqk 308 37 more more RBR cord-104269-9r7rqqqk 308 38 slowly slowly RB cord-104269-9r7rqqqk 308 39 . . . cord-104269-9r7rqqqk 309 1 Identification identification NN cord-104269-9r7rqqqk 309 2 of of IN cord-104269-9r7rqqqk 309 3 a a DT cord-104269-9r7rqqqk 309 4 sorting sorting NN cord-104269-9r7rqqqk 309 5 signal signal NN cord-104269-9r7rqqqk 309 6 in in IN cord-104269-9r7rqqqk 309 7 the the DT cord-104269-9r7rqqqk 309 8 Ii Ii NNP cord-104269-9r7rqqqk 309 9 transmembrane transmembrane NNP cord-104269-9r7rqqqk 309 10 region region NN cord-104269-9r7rqqqk 309 11 that that WDT cord-104269-9r7rqqqk 309 12 complements complement VBZ cord-104269-9r7rqqqk 309 13 the the DT cord-104269-9r7rqqqk 309 14 non non JJ cord-104269-9r7rqqqk 309 15 - - JJ cord-104269-9r7rqqqk 309 16 tyrosine tyrosine NN cord-104269-9r7rqqqk 309 17 - - HYPH cord-104269-9r7rqqqk 309 18 based base VBN cord-104269-9r7rqqqk 309 19 sorting sorting NN cord-104269-9r7rqqqk 309 20 signals signal NNS cord-104269-9r7rqqqk 309 21 in in IN cord-104269-9r7rqqqk 309 22 the the DT cord-104269-9r7rqqqk 309 23 Ii Ii NNP cord-104269-9r7rqqqk 309 24 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 309 25 tail tail NN cord-104269-9r7rqqqk 309 26 provides provide VBZ cord-104269-9r7rqqqk 309 27 a a DT cord-104269-9r7rqqqk 309 28 plausible plausible JJ cord-104269-9r7rqqqk 309 29 explanation explanation NN cord-104269-9r7rqqqk 309 30 for for IN cord-104269-9r7rqqqk 309 31 two two CD cord-104269-9r7rqqqk 309 32 earlier early JJR cord-104269-9r7rqqqk 309 33 observations observation NNS cord-104269-9r7rqqqk 309 34 . . . cord-104269-9r7rqqqk 310 1 Pieters Pieters NNP cord-104269-9r7rqqqk 310 2 et et NNP cord-104269-9r7rqqqk 310 3 al al NNP cord-104269-9r7rqqqk 310 4 . . . cord-104269-9r7rqqqk 311 1 ( ( -LRB- cord-104269-9r7rqqqk 311 2 1993 1993 CD cord-104269-9r7rqqqk 311 3 ) ) -RRB- cord-104269-9r7rqqqk 311 4 showed show VBD cord-104269-9r7rqqqk 311 5 that that IN cord-104269-9r7rqqqk 311 6 the the DT cord-104269-9r7rqqqk 311 7 Ii Ii NNP cord-104269-9r7rqqqk 311 8 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 311 9 tall tall JJ cord-104269-9r7rqqqk 311 10 was be VBD cord-104269-9r7rqqqk 311 11 sufficient sufficient JJ cord-104269-9r7rqqqk 311 12 to to TO cord-104269-9r7rqqqk 311 13 target target VB cord-104269-9r7rqqqk 311 14 a a DT cord-104269-9r7rqqqk 311 15 heterologous heterologous JJ cord-104269-9r7rqqqk 311 16 protein protein NN cord-104269-9r7rqqqk 311 17 , , , cord-104269-9r7rqqqk 311 18 influenza influenza NN cord-104269-9r7rqqqk 311 19 neuraminidase neuraminidase NN cord-104269-9r7rqqqk 311 20 , , , cord-104269-9r7rqqqk 311 21 to to IN cord-104269-9r7rqqqk 311 22 the the DT cord-104269-9r7rqqqk 311 23 endocytic endocytic JJ cord-104269-9r7rqqqk 311 24 pathway pathway NN cord-104269-9r7rqqqk 311 25 . . . cord-104269-9r7rqqqk 312 1 However however RB cord-104269-9r7rqqqk 312 2 , , , cord-104269-9r7rqqqk 312 3 they -PRON- PRP cord-104269-9r7rqqqk 312 4 noted note VBD cord-104269-9r7rqqqk 312 5 significant significant JJ cord-104269-9r7rqqqk 312 6 differences difference NNS cord-104269-9r7rqqqk 312 7 between between IN cord-104269-9r7rqqqk 312 8 the the DT cord-104269-9r7rqqqk 312 9 localization localization NN cord-104269-9r7rqqqk 312 10 of of IN cord-104269-9r7rqqqk 312 11 the the DT cord-104269-9r7rqqqk 312 12 Ii Ii NNP cord-104269-9r7rqqqk 312 13 - - HYPH cord-104269-9r7rqqqk 312 14 neuraminidase neuraminidase NN cord-104269-9r7rqqqk 312 15 chimera chimera NN cord-104269-9r7rqqqk 312 16 and and CC cord-104269-9r7rqqqk 312 17 Ii Ii NNP cord-104269-9r7rqqqk 312 18 within within IN cord-104269-9r7rqqqk 312 19 the the DT cord-104269-9r7rqqqk 312 20 endocytic endocytic JJ cord-104269-9r7rqqqk 312 21 pathway pathway NN cord-104269-9r7rqqqk 312 22 and and CC cord-104269-9r7rqqqk 312 23 concluded conclude VBD cord-104269-9r7rqqqk 312 24 that that IN cord-104269-9r7rqqqk 312 25 other other JJ cord-104269-9r7rqqqk 312 26 sorting sort VBG cord-104269-9r7rqqqk 312 27 information information NN cord-104269-9r7rqqqk 312 28 may may MD cord-104269-9r7rqqqk 312 29 be be VB cord-104269-9r7rqqqk 312 30 contained contain VBN cord-104269-9r7rqqqk 312 31 within within IN cord-104269-9r7rqqqk 312 32 the the DT cord-104269-9r7rqqqk 312 33 Ii Ii NNP cord-104269-9r7rqqqk 312 34 transmembrane transmembrane NNP cord-104269-9r7rqqqk 312 35 region region NN cord-104269-9r7rqqqk 312 36 and/or and/or CC cord-104269-9r7rqqqk 312 37 extracellular extracellular JJ cord-104269-9r7rqqqk 312 38 domain domain NN cord-104269-9r7rqqqk 312 39 . . . cord-104269-9r7rqqqk 313 1 In in IN cord-104269-9r7rqqqk 313 2 a a DT cord-104269-9r7rqqqk 313 3 second second JJ cord-104269-9r7rqqqk 313 4 study study NN cord-104269-9r7rqqqk 313 5 , , , cord-104269-9r7rqqqk 313 6 Nilsson Nilsson NNP cord-104269-9r7rqqqk 313 7 et et NNP cord-104269-9r7rqqqk 313 8 al al NNP cord-104269-9r7rqqqk 313 9 . . . cord-104269-9r7rqqqk 314 1 ( ( -LRB- cord-104269-9r7rqqqk 314 2 1991 1991 CD cord-104269-9r7rqqqk 314 3 ) ) -RRB- cord-104269-9r7rqqqk 314 4 reported report VBD cord-104269-9r7rqqqk 314 5 that that IN cord-104269-9r7rqqqk 314 6 an an DT cord-104269-9r7rqqqk 314 7 Ii Ii NNP cord-104269-9r7rqqqk 314 8 chimera chimera NN cord-104269-9r7rqqqk 314 9 containing contain VBG cord-104269-9r7rqqqk 314 10 the the DT cord-104269-9r7rqqqk 314 11 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 314 12 tail tail NN cord-104269-9r7rqqqk 314 13 of of IN cord-104269-9r7rqqqk 314 14 8 8 CD cord-104269-9r7rqqqk 314 15 - - SYM cord-104269-9r7rqqqk 314 16 1,4 1,4 CD cord-104269-9r7rqqqk 314 17 galactosyltransferase galactosyltransferase NN cord-104269-9r7rqqqk 314 18 , , , cord-104269-9r7rqqqk 314 19 an an DT cord-104269-9r7rqqqk 314 20 enzyme enzyme NN cord-104269-9r7rqqqk 314 21 normally normally RB cord-104269-9r7rqqqk 314 22 localized localize VBN cord-104269-9r7rqqqk 314 23 to to IN cord-104269-9r7rqqqk 314 24 the the DT cord-104269-9r7rqqqk 314 25 trans trans NNP cord-104269-9r7rqqqk 314 26 - - NNP cord-104269-9r7rqqqk 314 27 Golgi Golgi NNP cord-104269-9r7rqqqk 314 28 by by IN cord-104269-9r7rqqqk 314 29 a a DT cord-104269-9r7rqqqk 314 30 retention retention NN cord-104269-9r7rqqqk 314 31 signal signal NN cord-104269-9r7rqqqk 314 32 within within IN cord-104269-9r7rqqqk 314 33 its -PRON- PRP$ cord-104269-9r7rqqqk 314 34 transmembrane transmembrane NN cord-104269-9r7rqqqk 314 35 region region NN cord-104269-9r7rqqqk 314 36 , , , cord-104269-9r7rqqqk 314 37 paradoxically paradoxically RB cord-104269-9r7rqqqk 314 38 had have VBD cord-104269-9r7rqqqk 314 39 a a DT cord-104269-9r7rqqqk 314 40 subcellular subcellular JJ cord-104269-9r7rqqqk 314 41 distribution distribution NN cord-104269-9r7rqqqk 314 42 similar similar JJ cord-104269-9r7rqqqk 314 43 to to IN cord-104269-9r7rqqqk 314 44 wild wild JJ cord-104269-9r7rqqqk 314 45 - - HYPH cord-104269-9r7rqqqk 314 46 type type NN cord-104269-9r7rqqqk 314 47 Ii Ii NNP cord-104269-9r7rqqqk 314 48 ( ( -LRB- cord-104269-9r7rqqqk 314 49 p31 p31 NNP cord-104269-9r7rqqqk 314 50 ) ) -RRB- cord-104269-9r7rqqqk 314 51 . . . cord-104269-9r7rqqqk 315 1 The the DT cord-104269-9r7rqqqk 315 2 galactosyltransferase galactosyltransferase NN cord-104269-9r7rqqqk 315 3 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 315 4 tall tall JJ cord-104269-9r7rqqqk 315 5 contains contain VBZ cord-104269-9r7rqqqk 315 6 a a DT cord-104269-9r7rqqqk 315 7 dileucine dileucine NN cord-104269-9r7rqqqk 315 8 sequence sequence NN cord-104269-9r7rqqqk 315 9 which which WDT cord-104269-9r7rqqqk 315 10 may may MD cord-104269-9r7rqqqk 315 11 function function VB cord-104269-9r7rqqqk 315 12 as as IN cord-104269-9r7rqqqk 315 13 a a DT cord-104269-9r7rqqqk 315 14 sorting sorting NN cord-104269-9r7rqqqk 315 15 signal signal NN cord-104269-9r7rqqqk 315 16 and and CC cord-104269-9r7rqqqk 315 17 when when WRB cord-104269-9r7rqqqk 315 18 complemented complement VBN cord-104269-9r7rqqqk 315 19 by by IN cord-104269-9r7rqqqk 315 20 the the DT cord-104269-9r7rqqqk 315 21 Ii Ii NNP cord-104269-9r7rqqqk 315 22 transmembrane transmembrane NNP cord-104269-9r7rqqqk 315 23 region region NN cord-104269-9r7rqqqk 315 24 signal signal NN cord-104269-9r7rqqqk 315 25 promote promote VBP cord-104269-9r7rqqqk 315 26 similar similar JJ cord-104269-9r7rqqqk 315 27 trafficking trafficking NN cord-104269-9r7rqqqk 315 28 to to IN cord-104269-9r7rqqqk 315 29 wild wild JJ cord-104269-9r7rqqqk 315 30 - - HYPH cord-104269-9r7rqqqk 315 31 type type NN cord-104269-9r7rqqqk 315 32 Ii Ii NNP cord-104269-9r7rqqqk 315 33 . . . cord-104269-9r7rqqqk 316 1 Finally finally RB cord-104269-9r7rqqqk 316 2 , , , cord-104269-9r7rqqqk 316 3 Dintzis Dintzis NNP cord-104269-9r7rqqqk 316 4 and and CC cord-104269-9r7rqqqk 316 5 Pfeffer Pfeffer NNP cord-104269-9r7rqqqk 316 6 ( ( -LRB- cord-104269-9r7rqqqk 316 7 1990 1990 CD cord-104269-9r7rqqqk 316 8 ) ) -RRB- cord-104269-9r7rqqqk 316 9 observed observe VBD cord-104269-9r7rqqqk 316 10 that that IN cord-104269-9r7rqqqk 316 11 the the DT cord-104269-9r7rqqqk 316 12 M6PR M6PR NNP cord-104269-9r7rqqqk 316 13 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 316 14 tall tall JJ cord-104269-9r7rqqqk 316 15 was be VBD cord-104269-9r7rqqqk 316 16 not not RB cord-104269-9r7rqqqk 316 17 sufficient sufficient JJ cord-104269-9r7rqqqk 316 18 to to TO cord-104269-9r7rqqqk 316 19 target target VB cord-104269-9r7rqqqk 316 20 the the DT cord-104269-9r7rqqqk 316 21 epidermal epidermal JJ cord-104269-9r7rqqqk 316 22 growth growth NN cord-104269-9r7rqqqk 316 23 factor factor NN cord-104269-9r7rqqqk 316 24 receptor receptor NN cord-104269-9r7rqqqk 316 25 to to IN cord-104269-9r7rqqqk 316 26 prelysosomes prelysosomes NNP cord-104269-9r7rqqqk 316 27 and and CC cord-104269-9r7rqqqk 316 28 suggested suggest VBD cord-104269-9r7rqqqk 316 29 that that IN cord-104269-9r7rqqqk 316 30 additional additional JJ cord-104269-9r7rqqqk 316 31 sorting sort VBG cord-104269-9r7rqqqk 316 32 information information NN cord-104269-9r7rqqqk 316 33 in in IN cord-104269-9r7rqqqk 316 34 the the DT cord-104269-9r7rqqqk 316 35 M6PR M6PR NNP cord-104269-9r7rqqqk 316 36 transmembrane transmembrane NN cord-104269-9r7rqqqk 316 37 region region NN cord-104269-9r7rqqqk 316 38 and/or and/or CC cord-104269-9r7rqqqk 316 39 extracellular extracellular JJ cord-104269-9r7rqqqk 316 40 domain domain NN cord-104269-9r7rqqqk 316 41 may may MD cord-104269-9r7rqqqk 316 42 be be VB cord-104269-9r7rqqqk 316 43 required require VBN cord-104269-9r7rqqqk 316 44 . . . cord-104269-9r7rqqqk 317 1 The the DT cord-104269-9r7rqqqk 317 2 identification identification NN cord-104269-9r7rqqqk 317 3 of of IN cord-104269-9r7rqqqk 317 4 the the DT cord-104269-9r7rqqqk 317 5 Ii Ii NNP cord-104269-9r7rqqqk 317 6 sorting sorting NN cord-104269-9r7rqqqk 317 7 signals signal NNS cord-104269-9r7rqqqk 317 8 required require VBN cord-104269-9r7rqqqk 317 9 for for IN cord-104269-9r7rqqqk 317 10 targeting target VBG cord-104269-9r7rqqqk 317 11 MHC MHC NNP cord-104269-9r7rqqqk 317 12 class class NN cord-104269-9r7rqqqk 317 13 H h NN cord-104269-9r7rqqqk 317 14 molecules molecule NNS cord-104269-9r7rqqqk 317 15 to to IN cord-104269-9r7rqqqk 317 16 the the DT cord-104269-9r7rqqqk 317 17 compartment compartment NN cord-104269-9r7rqqqk 317 18 where where WRB cord-104269-9r7rqqqk 317 19 they -PRON- PRP cord-104269-9r7rqqqk 317 20 encounter encounter VBP cord-104269-9r7rqqqk 317 21 antigen antigen NN cord-104269-9r7rqqqk 317 22 may may MD cord-104269-9r7rqqqk 317 23 have have VB cord-104269-9r7rqqqk 317 24 practical practical JJ cord-104269-9r7rqqqk 317 25 implications implication NNS cord-104269-9r7rqqqk 317 26 since since IN cord-104269-9r7rqqqk 317 27 , , , cord-104269-9r7rqqqk 317 28 in in IN cord-104269-9r7rqqqk 317 29 principle principle NN cord-104269-9r7rqqqk 317 30 , , , cord-104269-9r7rqqqk 317 31 it -PRON- PRP cord-104269-9r7rqqqk 317 32 should should MD cord-104269-9r7rqqqk 317 33 be be VB cord-104269-9r7rqqqk 317 34 possible possible JJ cord-104269-9r7rqqqk 317 35 to to TO cord-104269-9r7rqqqk 317 36 target target VB cord-104269-9r7rqqqk 317 37 endogenously endogenously RB cord-104269-9r7rqqqk 317 38 synthesized synthesize VBN cord-104269-9r7rqqqk 317 39 recombinant recombinant JJ cord-104269-9r7rqqqk 317 40 proteins protein NNS cord-104269-9r7rqqqk 317 41 containing contain VBG cord-104269-9r7rqqqk 317 42 Ii Ii NNP cord-104269-9r7rqqqk 317 43 sorting sorting NN cord-104269-9r7rqqqk 317 44 signals signal NNS cord-104269-9r7rqqqk 317 45 to to IN cord-104269-9r7rqqqk 317 46 this this DT cord-104269-9r7rqqqk 317 47 compartment compartment NN cord-104269-9r7rqqqk 317 48 for for IN cord-104269-9r7rqqqk 317 49 degradation degradation NN cord-104269-9r7rqqqk 317 50 and and CC cord-104269-9r7rqqqk 317 51 selective selective JJ cord-104269-9r7rqqqk 317 52 presentation presentation NN cord-104269-9r7rqqqk 317 53 by by IN cord-104269-9r7rqqqk 317 54 MHC MHC NNP cord-104269-9r7rqqqk 317 55 class class NN cord-104269-9r7rqqqk 317 56 II II NNP cord-104269-9r7rqqqk 317 57 to to IN cord-104269-9r7rqqqk 317 58 T T NNP cord-104269-9r7rqqqk 317 59 helper helper NN cord-104269-9r7rqqqk 317 60 cells cell NNS cord-104269-9r7rqqqk 317 61 . . . cord-104269-9r7rqqqk 318 1 Abbreviations abbreviation NNS cord-104269-9r7rqqqk 318 2 used use VBN cord-104269-9r7rqqqk 318 3 in in IN cord-104269-9r7rqqqk 318 4 this this DT cord-104269-9r7rqqqk 318 5 paper paper NN cord-104269-9r7rqqqk 318 6 : : : cord-104269-9r7rqqqk 318 7 CEF CEF NNP cord-104269-9r7rqqqk 318 8 , , , cord-104269-9r7rqqqk 318 9 chicken chicken NN cord-104269-9r7rqqqk 318 10 embryo embryo NN cord-104269-9r7rqqqk 318 11 fibroblasts fibroblast NNS cord-104269-9r7rqqqk 318 12 ; ; : cord-104269-9r7rqqqk 318 13 CT CT NNP cord-104269-9r7rqqqk 318 14 , , , cord-104269-9r7rqqqk 318 15 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 318 16 tail tail NN cord-104269-9r7rqqqk 318 17 Iicr Iicr NNP cord-104269-9r7rqqqk 318 18 , , , cord-104269-9r7rqqqk 318 19 the the DT cord-104269-9r7rqqqk 318 20 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 318 21 tail tail NN cord-104269-9r7rqqqk 318 22 of of IN cord-104269-9r7rqqqk 318 23 Ii Ii NNP cord-104269-9r7rqqqk 318 24 Ii Ii NNP cord-104269-9r7rqqqk 318 25 transmembrane transmembrane NNP cord-104269-9r7rqqqk 318 26 region region NN cord-104269-9r7rqqqk 318 27 M6PR M6PR NNP cord-104269-9r7rqqqk 318 28 , , , cord-104269-9r7rqqqk 318 29 mannose-6-phosphate mannose-6-phosphate NNP cord-104269-9r7rqqqk 318 30 receptor receptor NN cord-104269-9r7rqqqk 318 31 MCH MCH NNP cord-104269-9r7rqqqk 318 32 class class NN cord-104269-9r7rqqqk 318 33 H h NN cord-104269-9r7rqqqk 318 34 - - HYPH cord-104269-9r7rqqqk 318 35 associated associate VBN cord-104269-9r7rqqqk 318 36 invariant invariant JJ cord-104269-9r7rqqqk 318 37 chain chain NN cord-104269-9r7rqqqk 318 38 contains contain VBZ cord-104269-9r7rqqqk 318 39 a a DT cord-104269-9r7rqqqk 318 40 sorting sorting NN cord-104269-9r7rqqqk 318 41 signal signal NN cord-104269-9r7rqqqk 318 42 for for IN cord-104269-9r7rqqqk 318 43 endosomal endosomal NNP cord-104269-9r7rqqqk 318 44 compartments compartment NNS cord-104269-9r7rqqqk 319 1 The the DT cord-104269-9r7rqqqk 319 2 reaction reaction NN cord-104269-9r7rqqqk 319 3 of of IN cord-104269-9r7rqqqk 319 4 ferric ferric JJ cord-104269-9r7rqqqk 319 5 salts salt NNS cord-104269-9r7rqqqk 319 6 with with IN cord-104269-9r7rqqqk 319 7 transferrin transferrin NN cord-104269-9r7rqqqk 319 8 Defective defective JJ cord-104269-9r7rqqqk 319 9 major major JJ cord-104269-9r7rqqqk 319 10 histocompatibility histocompatibility NN cord-104269-9r7rqqqk 319 11 complex complex JJ cord-104269-9r7rqqqk 319 12 class class NN cord-104269-9r7rqqqk 319 13 II II NNP cord-104269-9r7rqqqk 319 14 assembly assembly NN cord-104269-9r7rqqqk 319 15 , , , cord-104269-9r7rqqqk 319 16 transport transport NN cord-104269-9r7rqqqk 319 17 , , , cord-104269-9r7rqqqk 319 18 peptide peptide NN cord-104269-9r7rqqqk 319 19 acquisition acquisition NN cord-104269-9r7rqqqk 319 20 , , , cord-104269-9r7rqqqk 319 21 and and CC cord-104269-9r7rqqqk 319 22 CD4 CD4 NNP cord-104269-9r7rqqqk 319 23 + + CC cord-104269-9r7rqqqk 319 24 T T NNP cord-104269-9r7rqqqk 319 25 cell cell NN cord-104269-9r7rqqqk 319 26 selection selection NN cord-104269-9r7rqqqk 319 27 in in IN cord-104269-9r7rqqqk 319 28 mice mouse NNS cord-104269-9r7rqqqk 319 29 lacking lack VBG cord-104269-9r7rqqqk 319 30 invariant invariant JJ cord-104269-9r7rqqqk 319 31 chain chain NN cord-104269-9r7rqqqk 319 32 expression expression NN cord-104269-9r7rqqqk 319 33 Role role NN cord-104269-9r7rqqqk 319 34 for for IN cord-104269-9r7rqqqk 319 35 intracellular intracellular JJ cord-104269-9r7rqqqk 319 36 proteases protease NNS cord-104269-9r7rqqqk 319 37 in in IN cord-104269-9r7rqqqk 319 38 the the DT cord-104269-9r7rqqqk 319 39 processing processing NN cord-104269-9r7rqqqk 319 40 and and CC cord-104269-9r7rqqqk 319 41 transport transport NN cord-104269-9r7rqqqk 319 42 of of IN cord-104269-9r7rqqqk 320 1 class class NNP cord-104269-9r7rqqqk 320 2 II II NNP cord-104269-9r7rqqqk 320 3 HLA HLA NNP cord-104269-9r7rqqqk 320 4 antigens antigen NNS cord-104269-9r7rqqqk 321 1 Lysosomal lysosomal JJ cord-104269-9r7rqqqk 321 2 acid acid NN cord-104269-9r7rqqqk 321 3 phosphatase phosphatase NN cord-104269-9r7rqqqk 321 4 is be VBZ cord-104269-9r7rqqqk 321 5 transported transport VBN cord-104269-9r7rqqqk 321 6 to to IN cord-104269-9r7rqqqk 321 7 lysosomes lysosome NNS cord-104269-9r7rqqqk 321 8 via via IN cord-104269-9r7rqqqk 321 9 the the DT cord-104269-9r7rqqqk 321 10 cell cell NN cord-104269-9r7rqqqk 321 11 surface surface NN cord-104269-9r7rqqqk 321 12 Transferrin Transferrin NNP cord-104269-9r7rqqqk 321 13 receptor receptor NN cord-104269-9r7rqqqk 321 14 internalization internalization NN cord-104269-9r7rqqqk 321 15 sequence sequence NN cord-104269-9r7rqqqk 322 1 YXRF YXRF NNP cord-104269-9r7rqqqk 322 2 implicates implicate VBZ cord-104269-9r7rqqqk 322 3 a a DT cord-104269-9r7rqqqk 322 4 type type NN cord-104269-9r7rqqqk 322 5 I -PRON- PRP cord-104269-9r7rqqqk 322 6 turn turn VBP cord-104269-9r7rqqqk 322 7 as as IN cord-104269-9r7rqqqk 322 8 the the DT cord-104269-9r7rqqqk 322 9 structural structural JJ cord-104269-9r7rqqqk 322 10 recognition recognition NN cord-104269-9r7rqqqk 322 11 motif motif NN cord-104269-9r7rqqqk 322 12 for for IN cord-104269-9r7rqqqk 322 13 endocytosis endocytosis NN cord-104269-9r7rqqqk 323 1 Intracellular Intracellular NNP cord-104269-9r7rqqqk 323 2 class class NN cord-104269-9r7rqqqk 323 3 II II NNP cord-104269-9r7rqqqk 323 4 HLA HLA NNP cord-104269-9r7rqqqk 323 5 antigens antigen NNS cord-104269-9r7rqqqk 323 6 are be VBP cord-104269-9r7rqqqk 323 7 accessible accessible JJ cord-104269-9r7rqqqk 323 8 to to IN cord-104269-9r7rqqqk 323 9 transferrin transferrin NN cord-104269-9r7rqqqk 323 10 - - HYPH cord-104269-9r7rqqqk 323 11 neuraminidase neuraminidase NN cord-104269-9r7rqqqk 323 12 conjugates conjugate NNS cord-104269-9r7rqqqk 323 13 internalized internalize VBN cord-104269-9r7rqqqk 323 14 by by IN cord-104269-9r7rqqqk 323 15 receptor receptor NN cord-104269-9r7rqqqk 323 16 - - HYPH cord-104269-9r7rqqqk 323 17 mediated mediate VBN cord-104269-9r7rqqqk 323 18 endocytosis endocytosis NN cord-104269-9r7rqqqk 324 1 The the DT cord-104269-9r7rqqqk 324 2 preparation preparation NN cord-104269-9r7rqqqk 324 3 and and CC cord-104269-9r7rqqqk 324 4 use use NN cord-104269-9r7rqqqk 324 5 of of IN cord-104269-9r7rqqqk 324 6 gold gold NN cord-104269-9r7rqqqk 324 7 probes probe NNS cord-104269-9r7rqqqk 325 1 The the DT cord-104269-9r7rqqqk 325 2 mannose mannose NN cord-104269-9r7rqqqk 325 3 6-phosphate 6-phosphate NN cord-104269-9r7rqqqk 325 4 receptor receptor NN cord-104269-9r7rqqqk 325 5 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 325 6 domain domain NN cord-104269-9r7rqqqk 325 7 is be VBZ cord-104269-9r7rqqqk 325 8 not not RB cord-104269-9r7rqqqk 325 9 sufficient sufficient JJ cord-104269-9r7rqqqk 325 10 to to TO cord-104269-9r7rqqqk 325 11 alter alter VB cord-104269-9r7rqqqk 325 12 the the DT cord-104269-9r7rqqqk 325 13 cellular cellular JJ cord-104269-9r7rqqqk 325 14 distribution distribution NN cord-104269-9r7rqqqk 325 15 of of IN cord-104269-9r7rqqqk 325 16 a a DT cord-104269-9r7rqqqk 325 17 chimeric chimeric JJ cord-104269-9r7rqqqk 325 18 EGF EGF NNP cord-104269-9r7rqqqk 325 19 receptor receptor NN cord-104269-9r7rqqqk 325 20 Iterative iterative JJ cord-104269-9r7rqqqk 325 21 fractionation fractionation NN cord-104269-9r7rqqqk 325 22 of of IN cord-104269-9r7rqqqk 325 23 recycling recycle VBG cord-104269-9r7rqqqk 325 24 receptors receptor NNS cord-104269-9r7rqqqk 325 25 from from IN cord-104269-9r7rqqqk 325 26 lysosomally lysosomally RB cord-104269-9r7rqqqk 325 27 destined destine VBN cord-104269-9r7rqqqk 325 28 ligands ligand NNS cord-104269-9r7rqqqk 325 29 in in IN cord-104269-9r7rqqqk 325 30 an an DT cord-104269-9r7rqqqk 325 31 early early JJ cord-104269-9r7rqqqk 325 32 sorting sorting NN cord-104269-9r7rqqqk 325 33 endosome endosome NN cord-104269-9r7rqqqk 325 34 Co co NN cord-104269-9r7rqqqk 325 35 - - NN cord-104269-9r7rqqqk 325 36 localization localization NN cord-104269-9r7rqqqk 325 37 of of IN cord-104269-9r7rqqqk 325 38 molecules molecule NNS cord-104269-9r7rqqqk 325 39 involved involve VBN cord-104269-9r7rqqqk 325 40 in in IN cord-104269-9r7rqqqk 325 41 antigen antigen NN cord-104269-9r7rqqqk 325 42 processing processing NN cord-104269-9r7rqqqk 325 43 and and CC cord-104269-9r7rqqqk 325 44 presentation presentation NN cord-104269-9r7rqqqk 325 45 in in IN cord-104269-9r7rqqqk 325 46 an an DT cord-104269-9r7rqqqk 325 47 early early JJ cord-104269-9r7rqqqk 325 48 endocytic endocytic JJ cord-104269-9r7rqqqk 325 49 compartment compartment NN cord-104269-9r7rqqqk 325 50 Immunogenic immunogenic JJ cord-104269-9r7rqqqk 325 51 peptides peptide NNS cord-104269-9r7rqqqk 325 52 bind bind VBP cord-104269-9r7rqqqk 325 53 to to IN cord-104269-9r7rqqqk 325 54 class class NN cord-104269-9r7rqqqk 325 55 II II NNP cord-104269-9r7rqqqk 325 56 MHC MHC NNP cord-104269-9r7rqqqk 325 57 molecules molecule NNS cord-104269-9r7rqqqk 325 58 in in IN cord-104269-9r7rqqqk 325 59 an an DT cord-104269-9r7rqqqk 325 60 early early JJ cord-104269-9r7rqqqk 325 61 lysosomal lysosomal JJ cord-104269-9r7rqqqk 325 62 compartment compartment NN cord-104269-9r7rqqqk 325 63 Transport Transport NNP cord-104269-9r7rqqqk 325 64 of of IN cord-104269-9r7rqqqk 325 65 the the DT cord-104269-9r7rqqqk 325 66 lysosomal lysosomal JJ cord-104269-9r7rqqqk 325 67 membrane membrane NN cord-104269-9r7rqqqk 325 68 glycoprotein glycoprotein NN cord-104269-9r7rqqqk 325 69 lgpl20 lgpl20 NNP cord-104269-9r7rqqqk 325 70 ( ( -LRB- cord-104269-9r7rqqqk 325 71 lgp lgp NNP cord-104269-9r7rqqqk 325 72 - - HYPH cord-104269-9r7rqqqk 325 73 A A NNP cord-104269-9r7rqqqk 325 74 ) ) -RRB- cord-104269-9r7rqqqk 325 75 to to IN cord-104269-9r7rqqqk 325 76 lysosomes lysosome NNS cord-104269-9r7rqqqk 325 77 does do VBZ cord-104269-9r7rqqqk 325 78 not not RB cord-104269-9r7rqqqk 325 79 require require VB cord-104269-9r7rqqqk 325 80 appearance appearance NN cord-104269-9r7rqqqk 325 81 on on IN cord-104269-9r7rqqqk 325 82 the the DT cord-104269-9r7rqqqk 325 83 plasma plasma NN cord-104269-9r7rqqqk 325 84 membrane membrane NN cord-104269-9r7rqqqk 325 85 Intracellular Intracellular NNP cord-104269-9r7rqqqk 325 86 routing route VBG cord-104269-9r7rqqqk 325 87 of of IN cord-104269-9r7rqqqk 325 88 transferrin transferrin NN cord-104269-9r7rqqqk 325 89 and and CC cord-104269-9r7rqqqk 325 90 transferrin transferrin NN cord-104269-9r7rqqqk 325 91 receptors receptor NNS cord-104269-9r7rqqqk 325 92 in in IN cord-104269-9r7rqqqk 325 93 epidermoid epidermoid JJ cord-104269-9r7rqqqk 325 94 carcinoma carcinoma NN cord-104269-9r7rqqqk 325 95 A431 a431 CD cord-104269-9r7rqqqk 325 96 cells cell NNS cord-104269-9r7rqqqk 325 97 Internalization internalization NN cord-104269-9r7rqqqk 325 98 and and CC cord-104269-9r7rqqqk 325 99 processing processing NN cord-104269-9r7rqqqk 325 100 of of IN cord-104269-9r7rqqqk 325 101 transferrin transferrin NN cord-104269-9r7rqqqk 325 102 and and CC cord-104269-9r7rqqqk 325 103 the the DT cord-104269-9r7rqqqk 325 104 transferrin transferrin NN cord-104269-9r7rqqqk 325 105 receptor receptor NN cord-104269-9r7rqqqk 325 106 in in IN cord-104269-9r7rqqqk 325 107 human human JJ cord-104269-9r7rqqqk 325 108 carcinoma carcinoma NN cord-104269-9r7rqqqk 325 109 A431 a431 CD cord-104269-9r7rqqqk 325 110 cells cell NNS cord-104269-9r7rqqqk 325 111 Trowbridge Trowbridge NNP cord-104269-9r7rqqqk 325 112 . . . cord-104269-9r7rqqqk 326 1 1994 1994 CD cord-104269-9r7rqqqk 326 2 . . . cord-104269-9r7rqqqk 327 1 In in IN cord-104269-9r7rqqqk 327 2 migrating migrate VBG cord-104269-9r7rqqqk 327 3 fibroblasts fibroblast NNS cord-104269-9r7rqqqk 327 4 recycling recycling NN cord-104269-9r7rqqqk 327 5 receptors receptor NNS cord-104269-9r7rqqqk 327 6 are be VBP cord-104269-9r7rqqqk 327 7 concentrated concentrate VBN cord-104269-9r7rqqqk 327 8 in in IN cord-104269-9r7rqqqk 327 9 narrow narrow JJ cord-104269-9r7rqqqk 327 10 tubules tubule NNS cord-104269-9r7rqqqk 327 11 in in IN cord-104269-9r7rqqqk 327 12 the the DT cord-104269-9r7rqqqk 327 13 pericentriolar pericentriolar NNP cord-104269-9r7rqqqk 327 14 area area NN cord-104269-9r7rqqqk 327 15 and and CC cord-104269-9r7rqqqk 327 16 then then RB cord-104269-9r7rqqqk 327 17 routed route VBD cord-104269-9r7rqqqk 327 18 to to IN cord-104269-9r7rqqqk 327 19 the the DT cord-104269-9r7rqqqk 327 20 plasma plasma NN cord-104269-9r7rqqqk 327 21 membrane membrane NN cord-104269-9r7rqqqk 327 22 of of IN cord-104269-9r7rqqqk 327 23 the the DT cord-104269-9r7rqqqk 327 24 leading lead VBG cord-104269-9r7rqqqk 327 25 lameUa lameUa NNP cord-104269-9r7rqqqk 327 26 Vectors Vectors NNPS cord-104269-9r7rqqqk 327 27 and and CC cord-104269-9r7rqqqk 327 28 genes gene NNS cord-104269-9r7rqqqk 327 29 for for IN cord-104269-9r7rqqqk 327 30 improvement improvement NN cord-104269-9r7rqqqk 327 31 of of IN cord-104269-9r7rqqqk 327 32 animal animal NN cord-104269-9r7rqqqk 327 33 strains strain NNS cord-104269-9r7rqqqk 327 34 Nonacylated nonacylated JJ cord-104269-9r7rqqqk 327 35 human human JJ cord-104269-9r7rqqqk 327 36 transferrin transferrin NN cord-104269-9r7rqqqk 327 37 receptors receptor NNS cord-104269-9r7rqqqk 327 38 are be VBP cord-104269-9r7rqqqk 327 39 rapidly rapidly RB cord-104269-9r7rqqqk 327 40 internalized internalize VBN cord-104269-9r7rqqqk 327 41 and and CC cord-104269-9r7rqqqk 327 42 mediate mediate VB cord-104269-9r7rqqqk 327 43 iron iron NN cord-104269-9r7rqqqk 327 44 uptake uptake NN cord-104269-9r7rqqqk 327 45 Role role NN cord-104269-9r7rqqqk 327 46 of of IN cord-104269-9r7rqqqk 327 47 the the DT cord-104269-9r7rqqqk 327 48 human human JJ cord-104269-9r7rqqqk 327 49 transferrin transferrin NN cord-104269-9r7rqqqk 327 50 receptor receptor NN cord-104269-9r7rqqqk 327 51 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 327 52 domain domain NN cord-104269-9r7rqqqk 327 53 in in IN cord-104269-9r7rqqqk 327 54 endocytosis endocytosis NN cord-104269-9r7rqqqk 327 55 : : : cord-104269-9r7rqqqk 327 56 localization localization NN cord-104269-9r7rqqqk 327 57 of of IN cord-104269-9r7rqqqk 327 58 a a DT cord-104269-9r7rqqqk 327 59 specific specific JJ cord-104269-9r7rqqqk 327 60 signal signal NN cord-104269-9r7rqqqk 327 61 sequence sequence NN cord-104269-9r7rqqqk 327 62 for for IN cord-104269-9r7rqqqk 327 63 internalization internalization NN cord-104269-9r7rqqqk 328 1 A a DT cord-104269-9r7rqqqk 328 2 His His NNP cord-104269-9r7rqqqk 328 3 - - HYPH cord-104269-9r7rqqqk 328 4 Leu Leu NNP cord-104269-9r7rqqqk 328 5 - - HYPH cord-104269-9r7rqqqk 328 6 Leu Leu NNP cord-104269-9r7rqqqk 328 7 sequence sequence NN cord-104269-9r7rqqqk 328 8 near near IN cord-104269-9r7rqqqk 328 9 the the DT cord-104269-9r7rqqqk 328 10 carboxyl carboxyl NN cord-104269-9r7rqqqk 328 11 terminus terminus NN cord-104269-9r7rqqqk 328 12 of of IN cord-104269-9r7rqqqk 328 13 the the DT cord-104269-9r7rqqqk 328 14 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 328 15 domain domain NN cord-104269-9r7rqqqk 328 16 of of IN cord-104269-9r7rqqqk 328 17 the the DT cord-104269-9r7rqqqk 328 18 cation cation NN cord-104269-9r7rqqqk 328 19 - - HYPH cord-104269-9r7rqqqk 328 20 dependent dependent JJ cord-104269-9r7rqqqk 328 21 Marmose Marmose NNP cord-104269-9r7rqqqk 328 22 6-pbosphate 6-pbosphate CD cord-104269-9r7rqqqk 328 23 receptor receptor NN cord-104269-9r7rqqqk 328 24 is be VBZ cord-104269-9r7rqqqk 328 25 necessary necessary JJ cord-104269-9r7rqqqk 328 26 for for IN cord-104269-9r7rqqqk 328 27 the the DT cord-104269-9r7rqqqk 328 28 lysosomal lysosomal JJ cord-104269-9r7rqqqk 328 29 enzyme enzyme NN cord-104269-9r7rqqqk 328 30 sorting sorting NN cord-104269-9r7rqqqk 328 31 function function NN cord-104269-9r7rqqqk 329 1 The the DT cord-104269-9r7rqqqk 329 2 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 329 3 taft taft NN cord-104269-9r7rqqqk 329 4 of of IN cord-104269-9r7rqqqk 329 5 the the DT cord-104269-9r7rqqqk 329 6 Marmose Marmose NNP cord-104269-9r7rqqqk 329 7 6-phosphate 6-phosphate CD cord-104269-9r7rqqqk 329 8 / / SYM cord-104269-9r7rqqqk 329 9 insulin insulin NN cord-104269-9r7rqqqk 329 10 - - HYPH cord-104269-9r7rqqqk 329 11 like like JJ cord-104269-9r7rqqqk 329 12 growth growth NN cord-104269-9r7rqqqk 329 13 factor factor NN cord-104269-9r7rqqqk 329 14 - - HYPH cord-104269-9r7rqqqk 329 15 H h NN cord-104269-9r7rqqqk 329 16 receptor receptor NN cord-104269-9r7rqqqk 329 17 has have VBZ cord-104269-9r7rqqqk 329 18 two two CD cord-104269-9r7rqqqk 329 19 signals signal NNS cord-104269-9r7rqqqk 329 20 for for IN cord-104269-9r7rqqqk 329 21 lysosomal lysosomal JJ cord-104269-9r7rqqqk 329 22 enzyme enzyme NN cord-104269-9r7rqqqk 329 23 sorting sorting NN cord-104269-9r7rqqqk 329 24 in in IN cord-104269-9r7rqqqk 329 25 the the DT cord-104269-9r7rqqqk 329 26 Golgi Golgi NNP cord-104269-9r7rqqqk 329 27 New New NNP cord-104269-9r7rqqqk 329 28 procedure procedure NN cord-104269-9r7rqqqk 329 29 for for IN cord-104269-9r7rqqqk 329 30 DNA dna NN cord-104269-9r7rqqqk 329 31 transfection transfection NN cord-104269-9r7rqqqk 329 32 with with IN cord-104269-9r7rqqqk 329 33 polycation polycation NN cord-104269-9r7rqqqk 329 34 and and CC cord-104269-9r7rqqqk 329 35 dimethyl dimethyl NN cord-104269-9r7rqqqk 329 36 sulfoxide sulfoxide NN cord-104269-9r7rqqqk 329 37 Differences difference NNS cord-104269-9r7rqqqk 329 38 in in IN cord-104269-9r7rqqqk 329 39 the the DT cord-104269-9r7rqqqk 329 40 endosomal endosomal NNP cord-104269-9r7rqqqk 329 41 distributions distribution NNS cord-104269-9r7rqqqk 329 42 of of IN cord-104269-9r7rqqqk 329 43 the the DT cord-104269-9r7rqqqk 329 44 two two CD cord-104269-9r7rqqqk 329 45 Mannose Mannose NNP cord-104269-9r7rqqqk 329 46 6-phosphate 6-phosphate NNP cord-104269-9r7rqqqk 329 47 receptors receptor NNS cord-104269-9r7rqqqk 329 48 Primary primary JJ cord-104269-9r7rqqqk 329 49 structure structure NN cord-104269-9r7rqqqk 329 50 of of IN cord-104269-9r7rqqqk 329 51 the the DT cord-104269-9r7rqqqk 329 52 gene gene NN cord-104269-9r7rqqqk 329 53 for for IN cord-104269-9r7rqqqk 329 54 the the DT cord-104269-9r7rqqqk 329 55 murine murine JJ cord-104269-9r7rqqqk 329 56 Ia Ia NNP cord-104269-9r7rqqqk 329 57 antigen antigen NN cord-104269-9r7rqqqk 329 58 - - HYPH cord-104269-9r7rqqqk 329 59 associated associate VBN cord-104269-9r7rqqqk 329 60 in in IN cord-104269-9r7rqqqk 329 61 variant variant JJ cord-104269-9r7rqqqk 329 62 chains chain NNS cord-104269-9r7rqqqk 329 63 ( ( -LRB- cord-104269-9r7rqqqk 329 64 Ii Ii NNP cord-104269-9r7rqqqk 329 65 ) ) -RRB- cord-104269-9r7rqqqk 329 66 . . . cord-104269-9r7rqqqk 330 1 An an DT cord-104269-9r7rqqqk 330 2 alternatively alternatively RB cord-104269-9r7rqqqk 330 3 spliced splice VBN cord-104269-9r7rqqqk 330 4 exon exon NN cord-104269-9r7rqqqk 330 5 encodes encode VBZ cord-104269-9r7rqqqk 330 6 a a DT cord-104269-9r7rqqqk 330 7 cysteine cysteine JJ cord-104269-9r7rqqqk 330 8 rich rich JJ cord-104269-9r7rqqqk 330 9 domain domain NN cord-104269-9r7rqqqk 330 10 highly highly RB cord-104269-9r7rqqqk 330 11 homologous homologous JJ cord-104269-9r7rqqqk 330 12 to to IN cord-104269-9r7rqqqk 330 13 a a DT cord-104269-9r7rqqqk 330 14 repetitive repetitive JJ cord-104269-9r7rqqqk 330 15 sequence sequence NN cord-104269-9r7rqqqk 330 16 in in IN cord-104269-9r7rqqqk 330 17 thyroglobulin thyroglobulin NNP cord-104269-9r7rqqqk 331 1 The the DT cord-104269-9r7rqqqk 331 2 biogenesis biogenesis NN cord-104269-9r7rqqqk 331 3 of of IN cord-104269-9r7rqqqk 331 4 lysosomes lysosome NNS cord-104269-9r7rqqqk 331 5 Rapid Rapid NNP cord-104269-9r7rqqqk 331 6 and and CC cord-104269-9r7rqqqk 331 7 efficient efficient JJ cord-104269-9r7rqqqk 331 8 site site NN cord-104269-9r7rqqqk 331 9 - - HYPH cord-104269-9r7rqqqk 331 10 specific specific JJ cord-104269-9r7rqqqk 331 11 mutagenesis mutagenesis NN cord-104269-9r7rqqqk 331 12 without without IN cord-104269-9r7rqqqk 331 13 phenotypic phenotypic NN cord-104269-9r7rqqqk 331 14 selection selection NN cord-104269-9r7rqqqk 331 15 Invariant invariant JJ cord-104269-9r7rqqqk 331 16 chain chain NN cord-104269-9r7rqqqk 331 17 targets target VBZ cord-104269-9r7rqqqk 331 18 HLA HLA NNP cord-104269-9r7rqqqk 331 19 class class NN cord-104269-9r7rqqqk 331 20 II ii NN cord-104269-9r7rqqqk 331 21 molecules molecule NNS cord-104269-9r7rqqqk 331 22 to to IN cord-104269-9r7rqqqk 331 23 acidic acidic JJ cord-104269-9r7rqqqk 331 24 endosomes endosome NNS cord-104269-9r7rqqqk 331 25 containing contain VBG cord-104269-9r7rqqqk 331 26 internaiized internaiize VBN cord-104269-9r7rqqqk 331 27 influenza influenza NN cord-104269-9r7rqqqk 331 28 virus virus NN cord-104269-9r7rqqqk 331 29 A a DT cord-104269-9r7rqqqk 331 30 novel novel NN cord-104269-9r7rqqqk 331 31 di di NN cord-104269-9r7rqqqk 331 32 - - HYPH cord-104269-9r7rqqqk 331 33 leucine leucine NN cord-104269-9r7rqqqk 331 34 motif motif NN cord-104269-9r7rqqqk 331 35 and and CC cord-104269-9r7rqqqk 331 36 a a DT cord-104269-9r7rqqqk 331 37 tyrosine tyrosine NN cord-104269-9r7rqqqk 331 38 - - HYPH cord-104269-9r7rqqqk 331 39 based base VBN cord-104269-9r7rqqqk 331 40 motif motif NN cord-104269-9r7rqqqk 331 41 independently independently RB cord-104269-9r7rqqqk 331 42 mediate mediate VBP cord-104269-9r7rqqqk 331 43 lysosomal lysosomal NN cord-104269-9r7rqqqk 331 44 targeting targeting NN cord-104269-9r7rqqqk 331 45 and and CC cord-104269-9r7rqqqk 331 46 endocytosis endocytosis NN cord-104269-9r7rqqqk 331 47 of of IN cord-104269-9r7rqqqk 331 48 CD3 CD3 NNP cord-104269-9r7rqqqk 331 49 chains chain NNS cord-104269-9r7rqqqk 332 1 Lysosomal Lysosomal NNP cord-104269-9r7rqqqk 332 2 membrane membrane NN cord-104269-9r7rqqqk 332 3 dynamics dynamic NNS cord-104269-9r7rqqqk 332 4 : : : cord-104269-9r7rqqqk 333 1 structure structure NN cord-104269-9r7rqqqk 333 2 and and CC cord-104269-9r7rqqqk 333 3 interorganellar interorganellar JJ cord-104269-9r7rqqqk 333 4 movement movement NN cord-104269-9r7rqqqk 333 5 of of IN cord-104269-9r7rqqqk 333 6 a a DT cord-104269-9r7rqqqk 333 7 major major JJ cord-104269-9r7rqqqk 333 8 lysosornal lysosornal JJ cord-104269-9r7rqqqk 333 9 membrane membrane NN cord-104269-9r7rqqqk 333 10 glycoprotein glycoprotein NNP cord-104269-9r7rqqqk 333 11 Cycling Cycling NNP cord-104269-9r7rqqqk 333 12 of of IN cord-104269-9r7rqqqk 333 13 the the DT cord-104269-9r7rqqqk 333 14 integral integral JJ cord-104269-9r7rqqqk 333 15 membrane membrane NN cord-104269-9r7rqqqk 333 16 glycoprotein glycoprotein NN cord-104269-9r7rqqqk 333 17 , , , cord-104269-9r7rqqqk 333 18 LEP100 lep100 UH cord-104269-9r7rqqqk 333 19 , , , cord-104269-9r7rqqqk 333 20 between between IN cord-104269-9r7rqqqk 333 21 plasma plasma NN cord-104269-9r7rqqqk 333 22 membrane membrane NN cord-104269-9r7rqqqk 333 23 and and CC cord-104269-9r7rqqqk 333 24 lysosomes lysosome NNS cord-104269-9r7rqqqk 333 25 : : : cord-104269-9r7rqqqk 333 26 kinetic kinetic JJ cord-104269-9r7rqqqk 333 27 and and CC cord-104269-9r7rqqqk 333 28 morphological morphological JJ cord-104269-9r7rqqqk 333 29 analysis analysis NN cord-104269-9r7rqqqk 334 1 Intracelhilar intracelhilar JJ cord-104269-9r7rqqqk 334 2 transport transport NN cord-104269-9r7rqqqk 334 3 of of IN cord-104269-9r7rqqqk 334 4 class class NN cord-104269-9r7rqqqk 334 5 II II NNP cord-104269-9r7rqqqk 334 6 MHC MHC NNP cord-104269-9r7rqqqk 334 7 molecules molecule NNS cord-104269-9r7rqqqk 334 8 directed direct VBN cord-104269-9r7rqqqk 334 9 by by IN cord-104269-9r7rqqqk 334 10 invariant invariant JJ cord-104269-9r7rqqqk 334 11 chain chain NN cord-104269-9r7rqqqk 334 12 Distribution distribution NN cord-104269-9r7rqqqk 334 13 of of IN cord-104269-9r7rqqqk 334 14 newly newly RB cord-104269-9r7rqqqk 334 15 synthesized synthesize VBN cord-104269-9r7rqqqk 334 16 lysosomal lysosomal JJ cord-104269-9r7rqqqk 334 17 enzymes enzyme NNS cord-104269-9r7rqqqk 334 18 in in IN cord-104269-9r7rqqqk 334 19 the the DT cord-104269-9r7rqqqk 334 20 endocytic endocytic JJ cord-104269-9r7rqqqk 334 21 pathway pathway NN cord-104269-9r7rqqqk 334 22 of of IN cord-104269-9r7rqqqk 334 23 normal normal JJ cord-104269-9r7rqqqk 334 24 rat rat NN cord-104269-9r7rqqqk 334 25 kidney kidney NN cord-104269-9r7rqqqk 334 26 cells cell NNS cord-104269-9r7rqqqk 335 1 A a DT cord-104269-9r7rqqqk 335 2 specific specific JJ cord-104269-9r7rqqqk 335 3 transmembrane transmembrane NN cord-104269-9r7rqqqk 335 4 domain domain NN cord-104269-9r7rqqqk 335 5 of of IN cord-104269-9r7rqqqk 335 6 a a DT cord-104269-9r7rqqqk 335 7 coronavirns coronavirn NNS cord-104269-9r7rqqqk 335 8 El El NNP cord-104269-9r7rqqqk 335 9 glycoprotein glycoprotein NN cord-104269-9r7rqqqk 335 10 is be VBZ cord-104269-9r7rqqqk 335 11 required require VBN cord-104269-9r7rqqqk 335 12 for for IN cord-104269-9r7rqqqk 335 13 its -PRON- PRP$ cord-104269-9r7rqqqk 335 14 retention retention NN cord-104269-9r7rqqqk 335 15 in in IN cord-104269-9r7rqqqk 335 16 the the DT cord-104269-9r7rqqqk 335 17 Golgi Golgi NNP cord-104269-9r7rqqqk 336 1 The the DT cord-104269-9r7rqqqk 336 2 pathway pathway NN cord-104269-9r7rqqqk 336 3 and and CC cord-104269-9r7rqqqk 336 4 targeting targeting NN cord-104269-9r7rqqqk 336 5 signal signal NN cord-104269-9r7rqqqk 336 6 for for IN cord-104269-9r7rqqqk 336 7 delivery delivery NN cord-104269-9r7rqqqk 336 8 of of IN cord-104269-9r7rqqqk 336 9 the the DT cord-104269-9r7rqqqk 336 10 integral integral JJ cord-104269-9r7rqqqk 336 11 membrane membrane NN cord-104269-9r7rqqqk 336 12 glycoprotein glycoprotein NN cord-104269-9r7rqqqk 336 13 LEP100 LEP100 NNPS cord-104269-9r7rqqqk 337 1 to to IN cord-104269-9r7rqqqk 337 2 lysosomes lysosome NNS cord-104269-9r7rqqqk 338 1 Phosphorylation Phosphorylation NNP cord-104269-9r7rqqqk 338 2 of of IN cord-104269-9r7rqqqk 338 3 the the DT cord-104269-9r7rqqqk 338 4 cation cation NN cord-104269-9r7rqqqk 338 5 - - HYPH cord-104269-9r7rqqqk 338 6 independent independent JJ cord-104269-9r7rqqqk 338 7 Mannose Mannose NNP cord-104269-9r7rqqqk 338 8 6-phosphate 6-phosphate NNP cord-104269-9r7rqqqk 338 9 receptor receptor NN cord-104269-9r7rqqqk 338 10 is be VBZ cord-104269-9r7rqqqk 338 11 closely closely RB cord-104269-9r7rqqqk 338 12 associated associate VBN cord-104269-9r7rqqqk 338 13 with with IN cord-104269-9r7rqqqk 338 14 its -PRON- PRP$ cord-104269-9r7rqqqk 338 15 exit exit NN cord-104269-9r7rqqqk 338 16 from from IN cord-104269-9r7rqqqk 338 17 the the DT cord-104269-9r7rqqqk 338 18 trans trans NNP cord-104269-9r7rqqqk 338 19 - - NNP cord-104269-9r7rqqqk 338 20 Golgi Golgi NNP cord-104269-9r7rqqqk 338 21 network network NN cord-104269-9r7rqqqk 339 1 Transferrin Transferrin NNP cord-104269-9r7rqqqk 339 2 receptors receptor NNS cord-104269-9r7rqqqk 339 3 promote promote VBP cord-104269-9r7rqqqk 339 4 the the DT cord-104269-9r7rqqqk 339 5 formation formation NN cord-104269-9r7rqqqk 339 6 of of IN cord-104269-9r7rqqqk 339 7 clathrin clathrin NN cord-104269-9r7rqqqk 339 8 lattices lattice NNS cord-104269-9r7rqqqk 339 9 Sequences Sequences NNPS cord-104269-9r7rqqqk 339 10 within within IN cord-104269-9r7rqqqk 339 11 and and CC cord-104269-9r7rqqqk 339 12 adjacent adjacent JJ cord-104269-9r7rqqqk 339 13 to to IN cord-104269-9r7rqqqk 339 14 the the DT cord-104269-9r7rqqqk 339 15 transmembrane transmembrane NN cord-104269-9r7rqqqk 339 16 segment segment NN cord-104269-9r7rqqqk 339 17 of of IN cord-104269-9r7rqqqk 339 18 a-2,6-sialyltransferase a-2,6-sialyltransferase NNP cord-104269-9r7rqqqk 339 19 specify specify VB cord-104269-9r7rqqqk 339 20 Golgi Golgi NNP cord-104269-9r7rqqqk 339 21 retention retention NN cord-104269-9r7rqqqk 339 22 . . . cord-104269-9r7rqqqk 340 1 EMBO EMBO NNP cord-104269-9r7rqqqk 341 1 ( ( -LRB- cord-104269-9r7rqqqk 341 2 Eur Eur NNP cord-104269-9r7rqqqk 341 3 Time Time NNP cord-104269-9r7rqqqk 341 4 course course NN cord-104269-9r7rqqqk 341 5 of of IN cord-104269-9r7rqqqk 341 6 intracellular intracellular JJ cord-104269-9r7rqqqk 341 7 associations association NNS cord-104269-9r7rqqqk 341 8 , , , cord-104269-9r7rqqqk 341 9 processing processing NN cord-104269-9r7rqqqk 341 10 , , , cord-104269-9r7rqqqk 341 11 and and CC cord-104269-9r7rqqqk 341 12 cleavages cleavage NNS cord-104269-9r7rqqqk 341 13 of of IN cord-104269-9r7rqqqk 341 14 Ii Ii NNP cord-104269-9r7rqqqk 341 15 forms form NNS cord-104269-9r7rqqqk 341 16 and and CC cord-104269-9r7rqqqk 341 17 class class NN cord-104269-9r7rqqqk 341 18 II II NNP cord-104269-9r7rqqqk 341 19 major major JJ cord-104269-9r7rqqqk 341 20 histocompatibility histocompatibility NN cord-104269-9r7rqqqk 341 21 complex complex NN cord-104269-9r7rqqqk 341 22 molecules molecule NNS cord-104269-9r7rqqqk 342 1 The the DT cord-104269-9r7rqqqk 342 2 membrane membrane NN cord-104269-9r7rqqqk 342 3 spanning span VBG cord-104269-9r7rqqqk 342 4 domain domain NN cord-104269-9r7rqqqk 342 5 of of IN cord-104269-9r7rqqqk 342 6 8 8 CD cord-104269-9r7rqqqk 342 7 - - SYM cord-104269-9r7rqqqk 342 8 1,4-galactosyltransferas¢ 1,4-galactosyltransferas¢ CD cord-104269-9r7rqqqk 343 1 specifies specify VBZ cord-104269-9r7rqqqk 343 2 trans trans NNP cord-104269-9r7rqqqk 343 3 Golgi Golgi NNP cord-104269-9r7rqqqk 343 4 localization localization NN cord-104269-9r7rqqqk 344 1 Lysosomal Lysosomal NNP cord-104269-9r7rqqqk 344 2 targeting target VBG cord-104269-9r7rqqqk 344 3 of of IN cord-104269-9r7rqqqk 344 4 Limp Limp NNP cord-104269-9r7rqqqk 344 5 II II NNP cord-104269-9r7rqqqk 344 6 membrane membrane NN cord-104269-9r7rqqqk 344 7 glycoprotein glycoprotein NN cord-104269-9r7rqqqk 344 8 requires require VBZ cord-104269-9r7rqqqk 344 9 a a DT cord-104269-9r7rqqqk 344 10 novel novel JJ cord-104269-9r7rqqqk 344 11 Leu Leu NNP cord-104269-9r7rqqqk 344 12 - - HYPH cord-104269-9r7rqqqk 344 13 lle lle NNP cord-104269-9r7rqqqk 344 14 motif motif NN cord-104269-9r7rqqqk 344 15 at at IN cord-104269-9r7rqqqk 344 16 a a DT cord-104269-9r7rqqqk 344 17 particular particular JJ cord-104269-9r7rqqqk 344 18 position position NN cord-104269-9r7rqqqk 344 19 in in IN cord-104269-9r7rqqqk 344 20 its -PRON- PRP$ cord-104269-9r7rqqqk 344 21 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 344 22 domain domain NN cord-104269-9r7rqqqk 344 23 Biosynthesis Biosynthesis NNP cord-104269-9r7rqqqk 344 24 of of IN cord-104269-9r7rqqqk 344 25 the the DT cord-104269-9r7rqqqk 344 26 human human JJ cord-104269-9r7rqqqk 344 27 transferrin transferrin NN cord-104269-9r7rqqqk 344 28 receptor receptor NN cord-104269-9r7rqqqk 344 29 in in IN cord-104269-9r7rqqqk 344 30 cultured cultured JJ cord-104269-9r7rqqqk 344 31 cells cell NNS cord-104269-9r7rqqqk 345 1 Four four CD cord-104269-9r7rqqqk 345 2 la la NN cord-104269-9r7rqqqk 345 3 invariant invariant JJ cord-104269-9r7rqqqk 345 4 chain chain NN cord-104269-9r7rqqqk 345 5 forms form NNS cord-104269-9r7rqqqk 345 6 derive derive VBP cord-104269-9r7rqqqk 345 7 from from IN cord-104269-9r7rqqqk 345 8 a a DT cord-104269-9r7rqqqk 345 9 single single JJ cord-104269-9r7rqqqk 345 10 gene gene NN cord-104269-9r7rqqqk 345 11 by by IN cord-104269-9r7rqqqk 345 12 alternate alternate JJ cord-104269-9r7rqqqk 345 13 splicing splicing NN cord-104269-9r7rqqqk 345 14 and and CC cord-104269-9r7rqqqk 345 15 alternate alternate JJ cord-104269-9r7rqqqk 345 16 initiation initiation NN cord-104269-9r7rqqqk 345 17 of of IN cord-104269-9r7rqqqk 345 18 transcription transcription NN cord-104269-9r7rqqqk 345 19 / / SYM cord-104269-9r7rqqqk 345 20 translation translation NN cord-104269-9r7rqqqk 346 1 Targeting target VBG cord-104269-9r7rqqqk 346 2 ofa ofa DT cord-104269-9r7rqqqk 346 3 lysosomal lysosomal JJ cord-104269-9r7rqqqk 346 4 membrane membrane NN cord-104269-9r7rqqqk 346 5 protein protein NN cord-104269-9r7rqqqk 346 6 : : : cord-104269-9r7rqqqk 346 7 a a DT cord-104269-9r7rqqqk 346 8 tyrosine tyrosine NN cord-104269-9r7rqqqk 346 9 - - HYPH cord-104269-9r7rqqqk 346 10 containing contain VBG cord-104269-9r7rqqqk 346 11 endocytosis endocytosis NN cord-104269-9r7rqqqk 346 12 signal signal NN cord-104269-9r7rqqqk 346 13 in in IN cord-104269-9r7rqqqk 346 14 the the DT cord-104269-9r7rqqqk 346 15 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 346 16 tail tail NN cord-104269-9r7rqqqk 346 17 of of IN cord-104269-9r7rqqqk 346 18 lysosomal lysosomal JJ cord-104269-9r7rqqqk 346 19 acid acid NN cord-104269-9r7rqqqk 346 20 phoaphatase phoaphatase NN cord-104269-9r7rqqqk 346 21 is be VBZ cord-104269-9r7rqqqk 346 22 necessary necessary JJ cord-104269-9r7rqqqk 346 23 and and CC cord-104269-9r7rqqqk 346 24 sufficient sufficient JJ cord-104269-9r7rqqqk 346 25 for for IN cord-104269-9r7rqqqk 346 26 targeting target VBG cord-104269-9r7rqqqk 346 27 to to IN cord-104269-9r7rqqqk 346 28 lysosomes lysosomes JJ cord-104269-9r7rqqqk 346 29 Segregation Segregation NNP cord-104269-9r7rqqqk 346 30 of of IN cord-104269-9r7rqqqk 346 31 MHC MHC NNP cord-104269-9r7rqqqk 346 32 class class NN cord-104269-9r7rqqqk 346 33 II II NNP cord-104269-9r7rqqqk 346 34 molecules molecule NNS cord-104269-9r7rqqqk 346 35 from from IN cord-104269-9r7rqqqk 346 36 MHC MHC NNP cord-104269-9r7rqqqk 346 37 class class NN cord-104269-9r7rqqqk 347 1 I -PRON- PRP cord-104269-9r7rqqqk 347 2 molecules molecule NNS cord-104269-9r7rqqqk 347 3 in in IN cord-104269-9r7rqqqk 347 4 the the DT cord-104269-9r7rqqqk 347 5 Golgi Golgi NNP cord-104269-9r7rqqqk 347 6 complex complex NN cord-104269-9r7rqqqk 347 7 for for IN cord-104269-9r7rqqqk 347 8 transport transport NN cord-104269-9r7rqqqk 347 9 to to IN cord-104269-9r7rqqqk 347 10 lysosomal lysosomal JJ cord-104269-9r7rqqqk 347 11 compartments compartment NNS cord-104269-9r7rqqqk 348 1 Intracellular intracellular JJ cord-104269-9r7rqqqk 348 2 transport transport NN cord-104269-9r7rqqqk 348 3 and and CC cord-104269-9r7rqqqk 348 4 localization localization NN cord-104269-9r7rqqqk 348 5 of of IN cord-104269-9r7rqqqk 348 6 major major JJ cord-104269-9r7rqqqk 348 7 histocompatibility histocompatibility NN cord-104269-9r7rqqqk 348 8 complex complex JJ cord-104269-9r7rqqqk 348 9 class class NN cord-104269-9r7rqqqk 348 10 H h NN cord-104269-9r7rqqqk 348 11 molecules molecule NNS cord-104269-9r7rqqqk 348 12 and and CC cord-104269-9r7rqqqk 348 13 associated associate VBD cord-104269-9r7rqqqk 348 14 invariant invariant JJ cord-104269-9r7rqqqk 348 15 chain chain NN cord-104269-9r7rqqqk 349 1 The the DT cord-104269-9r7rqqqk 349 2 MHC MHC NNP cord-104269-9r7rqqqk 349 3 class class NN cord-104269-9r7rqqqk 349 4 IIassociatext IIassociatext NNP cord-104269-9r7rqqqk 349 5 invariant invariant JJ cord-104269-9r7rqqqk 349 6 chain chain NN cord-104269-9r7rqqqk 349 7 contains contain VBZ cord-104269-9r7rqqqk 349 8 two two CD cord-104269-9r7rqqqk 349 9 endosomal endosomal NNP cord-104269-9r7rqqqk 349 10 targeting target VBG cord-104269-9r7rqqqk 349 11 signals signal NNS cord-104269-9r7rqqqk 349 12 within within IN cord-104269-9r7rqqqk 349 13 its -PRON- PRP$ cord-104269-9r7rqqqk 349 14 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 349 15 tail tail NN cord-104269-9r7rqqqk 349 16 Identification identification NN cord-104269-9r7rqqqk 349 17 of of IN cord-104269-9r7rqqqk 349 18 subcellular subcellular JJ cord-104269-9r7rqqqk 349 19 compartments compartment NNS cord-104269-9r7rqqqk 349 20 involved involve VBN cord-104269-9r7rqqqk 349 21 in in IN cord-104269-9r7rqqqk 349 22 biosynthetic biosynthetic JJ cord-104269-9r7rqqqk 349 23 processing processing NN cord-104269-9r7rqqqk 349 24 of of IN cord-104269-9r7rqqqk 349 25 cathepsin cathepsin NN cord-104269-9r7rqqqk 349 26 D D NNP cord-104269-9r7rqqqk 349 27 Invariant Invariant NNP cord-104269-9r7rqqqk 349 28 chain chain NN cord-104269-9r7rqqqk 349 29 association association NN cord-104269-9r7rqqqk 349 30 with with IN cord-104269-9r7rqqqk 349 31 HLA HLA NNP cord-104269-9r7rqqqk 349 32 - - HYPH cord-104269-9r7rqqqk 349 33 DR DR NNP cord-104269-9r7rqqqk 349 34 molecules molecule NNS cord-104269-9r7rqqqk 350 1 inhibits inhibit VBZ cord-104269-9r7rqqqk 350 2 immunogenic immunogenic JJ cord-104269-9r7rqqqk 350 3 peptide peptide NN cord-104269-9r7rqqqk 350 4 binding bind VBG cord-104269-9r7rqqqk 350 5 Stable stable JJ cord-104269-9r7rqqqk 350 6 surface surface NN cord-104269-9r7rqqqk 350 7 expression expression NN cord-104269-9r7rqqqk 350 8 of of IN cord-104269-9r7rqqqk 350 9 invariant invariant JJ cord-104269-9r7rqqqk 350 10 chain chain NN cord-104269-9r7rqqqk 350 11 prevents prevent VBZ cord-104269-9r7rqqqk 350 12 peptide peptide JJ cord-104269-9r7rqqqk 350 13 presentation presentation NN cord-104269-9r7rqqqk 350 14 by by IN cord-104269-9r7rqqqk 350 15 HLA HLA NNP cord-104269-9r7rqqqk 350 16 - - HYPH cord-104269-9r7rqqqk 350 17 DR DR NNP cord-104269-9r7rqqqk 350 18 Cell Cell NNP cord-104269-9r7rqqqk 350 19 surface surface NN cord-104269-9r7rqqqk 350 20 HLA HLA NNP cord-104269-9r7rqqqk 350 21 - - HYPH cord-104269-9r7rqqqk 350 22 DR DR NNP cord-104269-9r7rqqqk 350 23 - - HYPH cord-104269-9r7rqqqk 350 24 invariant invariant JJ cord-104269-9r7rqqqk 350 25 chain chain NN cord-104269-9r7rqqqk 350 26 complexes complex NNS cord-104269-9r7rqqqk 350 27 are be VBP cord-104269-9r7rqqqk 350 28 targeted target VBN cord-104269-9r7rqqqk 350 29 to to IN cord-104269-9r7rqqqk 350 30 endosomes endosome NNS cord-104269-9r7rqqqk 350 31 by by IN cord-104269-9r7rqqqk 350 32 rapid rapid JJ cord-104269-9r7rqqqk 350 33 internalization internalization NN cord-104269-9r7rqqqk 350 34 Relationship relationship NN cord-104269-9r7rqqqk 350 35 between between IN cord-104269-9r7rqqqk 350 36 invariant invariant JJ cord-104269-9r7rqqqk 350 37 chain chain NN cord-104269-9r7rqqqk 350 38 expression expression NN cord-104269-9r7rqqqk 350 39 and and CC cord-104269-9r7rqqqk 350 40 major major JJ cord-104269-9r7rqqqk 350 41 histocompatibility histocompatibility NN cord-104269-9r7rqqqk 350 42 complex complex JJ cord-104269-9r7rqqqk 350 43 class class NN cord-104269-9r7rqqqk 350 44 II ii CD cord-104269-9r7rqqqk 350 45 transport transport NN cord-104269-9r7rqqqk 350 46 into into IN cord-104269-9r7rqqqk 350 47 early early JJ cord-104269-9r7rqqqk 350 48 and and CC cord-104269-9r7rqqqk 350 49 late late JJ cord-104269-9r7rqqqk 350 50 endocytic endocytic JJ cord-104269-9r7rqqqk 350 51 compartments compartment NNS cord-104269-9r7rqqqk 350 52 DNA dna NN cord-104269-9r7rqqqk 350 53 sequencing sequencing NN cord-104269-9r7rqqqk 350 54 with with IN cord-104269-9r7rqqqk 350 55 chainterminating chainterminate VBG cord-104269-9r7rqqqk 350 56 inhibitors inhibitor NNS cord-104269-9r7rqqqk 351 1 Intracelhilar intracelhilar JJ cord-104269-9r7rqqqk 351 2 distribution distribution NN cord-104269-9r7rqqqk 351 3 of of IN cord-104269-9r7rqqqk 351 4 the the DT cord-104269-9r7rqqqk 351 5 MHC MHC NNP cord-104269-9r7rqqqk 351 6 class class NN cord-104269-9r7rqqqk 351 7 II II NNP cord-104269-9r7rqqqk 351 8 molecules molecule NNS cord-104269-9r7rqqqk 351 9 and and CC cord-104269-9r7rqqqk 351 10 the the DT cord-104269-9r7rqqqk 351 11 as as IN cord-104269-9r7rqqqk 351 12 - - HYPH cord-104269-9r7rqqqk 351 13 social social JJ cord-104269-9r7rqqqk 351 14 ~ ~ NNP cord-104269-9r7rqqqk 351 15 l l NN cord-104269-9r7rqqqk 351 16 invariant invariant JJ cord-104269-9r7rqqqk 351 17 chain chain NN cord-104269-9r7rqqqk 351 18 0i 0i CD cord-104269-9r7rqqqk 351 19 ) ) -RRB- cord-104269-9r7rqqqk 351 20 in in IN cord-104269-9r7rqqqk 351 21 different different JJ cord-104269-9r7rqqqk 351 22 cell cell NN cord-104269-9r7rqqqk 351 23 lines line NNS cord-104269-9r7rqqqk 352 1 A a DT cord-104269-9r7rqqqk 352 2 new new JJ cord-104269-9r7rqqqk 352 3 method method NN cord-104269-9r7rqqqk 352 4 of of IN cord-104269-9r7rqqqk 352 5 preparing prepare VBG cord-104269-9r7rqqqk 352 6 gold gold NN cord-104269-9r7rqqqk 352 7 probes probe NNS cord-104269-9r7rqqqk 352 8 for for IN cord-104269-9r7rqqqk 352 9 multiple multiple JJ cord-104269-9r7rqqqk 352 10 - - HYPH cord-104269-9r7rqqqk 352 11 labelling labelling NN cord-104269-9r7rqqqk 352 12 cytochemistry cytochemistry NN cord-104269-9r7rqqqk 352 13 Two two CD cord-104269-9r7rqqqk 352 14 forms form NNS cord-104269-9r7rqqqk 352 15 of of IN cord-104269-9r7rqqqk 352 16 the the DT cord-104269-9r7rqqqk 352 17 la la JJ cord-104269-9r7rqqqk 352 18 antigenassociated antigenassociated JJ cord-104269-9r7rqqqk 352 19 invariant invariant JJ cord-104269-9r7rqqqk 352 20 chain chain NN cord-104269-9r7rqqqk 352 21 result result NN cord-104269-9r7rqqqk 352 22 from from IN cord-104269-9r7rqqqk 352 23 alternative alternative JJ cord-104269-9r7rqqqk 352 24 initiations initiation NNS cord-104269-9r7rqqqk 352 25 at at IN cord-104269-9r7rqqqk 352 26 two two CD cord-104269-9r7rqqqk 352 27 in in IN cord-104269-9r7rqqqk 352 28 - - HYPH cord-104269-9r7rqqqk 352 29 phase phase NN cord-104269-9r7rqqqk 352 30 AUGs aug NNS cord-104269-9r7rqqqk 352 31 A a DT cord-104269-9r7rqqqk 352 32 Golgi Golgi NNP cord-104269-9r7rqqqk 352 33 retention retention NN cord-104269-9r7rqqqk 352 34 signal signal NN cord-104269-9r7rqqqk 352 35 in in IN cord-104269-9r7rqqqk 352 36 a a DT cord-104269-9r7rqqqk 352 37 membrane membrane NN cord-104269-9r7rqqqk 352 38 - - HYPH cord-104269-9r7rqqqk 352 39 spanning span VBG cord-104269-9r7rqqqk 352 40 domain domain NN cord-104269-9r7rqqqk 352 41 of of IN cord-104269-9r7rqqqk 352 42 coronavirus coronavirus NN cord-104269-9r7rqqqk 352 43 E1 e1 NN cord-104269-9r7rqqqk 352 44 protein protein NN cord-104269-9r7rqqqk 352 45 DNA dna NN cord-104269-9r7rqqqk 352 46 sequence sequence NN cord-104269-9r7rqqqk 352 47 analysis analysis NN cord-104269-9r7rqqqk 352 48 with with IN cord-104269-9r7rqqqk 352 49 a a DT cord-104269-9r7rqqqk 352 50 modified modify VBN cord-104269-9r7rqqqk 352 51 bacteriophage bacteriophage NN cord-104269-9r7rqqqk 352 52 T7 t7 NN cord-104269-9r7rqqqk 352 53 DNA dna NN cord-104269-9r7rqqqk 352 54 polymerase polymerase NN cord-104269-9r7rqqqk 353 1 Insulin insulin NN cord-104269-9r7rqqqk 353 2 elicits elicit VBZ cord-104269-9r7rqqqk 353 3 a a DT cord-104269-9r7rqqqk 353 4 redistribution redistribution NN cord-104269-9r7rqqqk 353 5 of of IN cord-104269-9r7rqqqk 353 6 transferrin transferrin NN cord-104269-9r7rqqqk 353 7 receptors receptor NNS cord-104269-9r7rqqqk 353 8 in in IN cord-104269-9r7rqqqk 353 9 3T3-L 3t3-l CD cord-104269-9r7rqqqk 353 10 1 1 CD cord-104269-9r7rqqqk 353 11 edipocytes edipocyte NNS cord-104269-9r7rqqqk 353 12 through through IN cord-104269-9r7rqqqk 353 13 an an DT cord-104269-9r7rqqqk 353 14 increase increase NN cord-104269-9r7rqqqk 353 15 in in IN cord-104269-9r7rqqqk 353 16 the the DT cord-104269-9r7rqqqk 353 17 rate rate NN cord-104269-9r7rqqqk 353 18 constant constant NN cord-104269-9r7rqqqk 353 19 for for IN cord-104269-9r7rqqqk 353 20 receptor receptor NN cord-104269-9r7rqqqk 353 21 externalization externalization NN cord-104269-9r7rqqqk 353 22 Invariant invariant JJ cord-104269-9r7rqqqk 353 23 chain chain NN cord-104269-9r7rqqqk 353 24 distinguishes distinguish VBZ cord-104269-9r7rqqqk 353 25 between between IN cord-104269-9r7rqqqk 353 26 the the DT cord-104269-9r7rqqqk 353 27 exogenous exogenous JJ cord-104269-9r7rqqqk 353 28 and and CC cord-104269-9r7rqqqk 353 29 endogenous endogenous JJ cord-104269-9r7rqqqk 353 30 antigen antigen NN cord-104269-9r7rqqqk 353 31 presentation presentation NN cord-104269-9r7rqqqk 353 32 pathways pathway NNS cord-104269-9r7rqqqk 354 1 A a DT cord-104269-9r7rqqqk 354 2 study study NN cord-104269-9r7rqqqk 354 3 of of IN cord-104269-9r7rqqqk 354 4 positive positive JJ cord-104269-9r7rqqqk 354 5 staining staining NN cord-104269-9r7rqqqk 354 6 of of IN cord-104269-9r7rqqqk 354 7 ultrathin ultrathin JJ cord-104269-9r7rqqqk 354 8 frozen frozen JJ cord-104269-9r7rqqqk 354 9 sections section NNS cord-104269-9r7rqqqk 354 10 Human human JJ cord-104269-9r7rqqqk 354 11 cell cell NN cord-104269-9r7rqqqk 354 12 surface surface NN cord-104269-9r7rqqqk 354 13 glycoprotein glycoprotein NN cord-104269-9r7rqqqk 354 14 related relate VBN cord-104269-9r7rqqqk 354 15 to to IN cord-104269-9r7rqqqk 354 16 cell cell NN cord-104269-9r7rqqqk 354 17 proliferation proliferation NN cord-104269-9r7rqqqk 354 18 is be VBZ cord-104269-9r7rqqqk 354 19 the the DT cord-104269-9r7rqqqk 354 20 receptor receptor NN cord-104269-9r7rqqqk 354 21 for for IN cord-104269-9r7rqqqk 354 22 transferrin transferrin NN cord-104269-9r7rqqqk 354 23 Signal signal NN cord-104269-9r7rqqqk 354 24 - - HYPH cord-104269-9r7rqqqk 354 25 dependent dependent JJ cord-104269-9r7rqqqk 354 26 membrane membrane NN cord-104269-9r7rqqqk 354 27 protein protein NN cord-104269-9r7rqqqk 354 28 trafficking trafficking NN cord-104269-9r7rqqqk 354 29 in in IN cord-104269-9r7rqqqk 354 30 the the DT cord-104269-9r7rqqqk 354 31 endocytic endocytic JJ cord-104269-9r7rqqqk 354 32 pathway pathway NN cord-104269-9r7rqqqk 354 33 Mice mouse NNS cord-104269-9r7rqqqk 354 34 lacking lack VBG cord-104269-9r7rqqqk 354 35 the the DT cord-104269-9r7rqqqk 354 36 MHC MHC NNP cord-104269-9r7rqqqk 354 37 class class NN cord-104269-9r7rqqqk 354 38 H h NN cord-104269-9r7rqqqk 354 39 - - HYPH cord-104269-9r7rqqqk 354 40 associated associate VBN cord-104269-9r7rqqqk 354 41 invariant invariant JJ cord-104269-9r7rqqqk 354 42 chain chain NN cord-104269-9r7rqqqk 354 43 Exposure exposure NN cord-104269-9r7rqqqk 354 44 of of IN cord-104269-9r7rqqqk 354 45 K562 k562 CD cord-104269-9r7rqqqk 354 46 cells cell NNS cord-104269-9r7rqqqk 354 47 to to IN cord-104269-9r7rqqqk 354 48 anti anti JJ cord-104269-9r7rqqqk 354 49 - - JJ cord-104269-9r7rqqqk 354 50 receptor receptor JJ cord-104269-9r7rqqqk 354 51 monoclonal monoclonal JJ cord-104269-9r7rqqqk 354 52 antibody antibody NN cord-104269-9r7rqqqk 354 53 OKT9 OKT9 NNP cord-104269-9r7rqqqk 354 54 results result NNS cord-104269-9r7rqqqk 354 55 in in IN cord-104269-9r7rqqqk 354 56 rapid rapid JJ cord-104269-9r7rqqqk 354 57 redistribution redistribution NN cord-104269-9r7rqqqk 354 58 and and CC cord-104269-9r7rqqqk 354 59 enhanced enhanced JJ cord-104269-9r7rqqqk 354 60 degradation degradation NN cord-104269-9r7rqqqk 354 61 of of IN cord-104269-9r7rqqqk 354 62 the the DT cord-104269-9r7rqqqk 354 63 transferrin transferrin NN cord-104269-9r7rqqqk 354 64 receptor receptor NN cord-104269-9r7rqqqk 354 65 Accumulation Accumulation NNP cord-104269-9r7rqqqk 354 66 of of IN cord-104269-9r7rqqqk 354 67 membrane membrane NN cord-104269-9r7rqqqk 354 68 glycoproteins glycoprotein NNS cord-104269-9r7rqqqk 354 69 in in IN cord-104269-9r7rqqqk 354 70 lysosomes lysosome NNS cord-104269-9r7rqqqk 354 71 requires require VBZ cord-104269-9r7rqqqk 354 72 a a DT cord-104269-9r7rqqqk 354 73 tyrosine tyrosine NN cord-104269-9r7rqqqk 354 74 residue residue NN cord-104269-9r7rqqqk 354 75 at at IN cord-104269-9r7rqqqk 354 76 a a DT cord-104269-9r7rqqqk 354 77 particular particular JJ cord-104269-9r7rqqqk 354 78 position position NN cord-104269-9r7rqqqk 354 79 in in IN cord-104269-9r7rqqqk 354 80 the the DT cord-104269-9r7rqqqk 354 81 cytoplasmic cytoplasmic JJ cord-104269-9r7rqqqk 354 82 tail tail NN cord-104269-9r7rqqqk 355 1 The the DT cord-104269-9r7rqqqk 355 2 17-residue 17-residue CD cord-104269-9r7rqqqk 355 3 transmembrane transmembrane NN cord-104269-9r7rqqqk 355 4 domain domain NN cord-104269-9r7rqqqk 355 5 of of IN cord-104269-9r7rqqqk 355 6 ~-galactoside ~-galactoside NN cord-104269-9r7rqqqk 355 7 o~2,6-sialyltransferase o~2,6-sialyltransferase NNP cord-104269-9r7rqqqk 355 8 is be VBZ cord-104269-9r7rqqqk 355 9 sufficient sufficient JJ cord-104269-9r7rqqqk 355 10 for for IN cord-104269-9r7rqqqk 355 11 Golgi Golgi NNP cord-104269-9r7rqqqk 355 12 retention retention NN cord-104269-9r7rqqqk 355 13 Human Human NNP cord-104269-9r7rqqqk 355 14 major major JJ cord-104269-9r7rqqqk 355 15 histecompatibility histecompatibility NN cord-104269-9r7rqqqk 355 16 complex complex JJ cord-104269-9r7rqqqk 355 17 class class NN cord-104269-9r7rqqqk 355 18 II ii NN cord-104269-9r7rqqqk 355 19 invariant invariant JJ cord-104269-9r7rqqqk 355 20 chain chain NN cord-104269-9r7rqqqk 355 21 is be VBZ cord-104269-9r7rqqqk 355 22 expressed express VBN cord-104269-9r7rqqqk 355 23 on on IN cord-104269-9r7rqqqk 355 24 the the DT cord-104269-9r7rqqqk 355 25 cell cell NN cord-104269-9r7rqqqk 355 26 surface surface NN cord-104269-9r7rqqqk 355 27 A a DT cord-104269-9r7rqqqk 355 28 block block NN cord-104269-9r7rqqqk 355 29 in in IN cord-104269-9r7rqqqk 355 30 degrad degrad NN cord-104269-9r7rqqqk 355 31 - - HYPH cord-104269-9r7rqqqk 355 32 tion tion NN cord-104269-9r7rqqqk 355 33 of of IN cord-104269-9r7rqqqk 355 34 MHC MHC NNP cord-104269-9r7rqqqk 355 35 class class NN cord-104269-9r7rqqqk 355 36 H h NN cord-104269-9r7rqqqk 355 37 - - HYPH cord-104269-9r7rqqqk 355 38 associated associate VBN cord-104269-9r7rqqqk 355 39 invariant invariant JJ cord-104269-9r7rqqqk 355 40 chain chain NN cord-104269-9r7rqqqk 355 41 correlates correlate VBZ cord-104269-9r7rqqqk 355 42 with with IN cord-104269-9r7rqqqk 355 43 a a DT cord-104269-9r7rqqqk 355 44 reduction reduction NN cord-104269-9r7rqqqk 355 45 in in IN cord-104269-9r7rqqqk 355 46 transport transport NN cord-104269-9r7rqqqk 355 47 from from IN cord-104269-9r7rqqqk 355 48 endosome endosome NN cord-104269-9r7rqqqk 355 49 carrier carrier NN cord-104269-9r7rqqqk 355 50 vesicles vesicle NNS cord-104269-9r7rqqqk 355 51 to to IN cord-104269-9r7rqqqk 355 52 the the DT cord-104269-9r7rqqqk 355 53 prelysosome prelysosome NNP cord-104269-9r7rqqqk 355 54 compartment compartment NN cord-104269-9r7rqqqk 356 1 We -PRON- PRP cord-104269-9r7rqqqk 356 2 thank thank VBP cord-104269-9r7rqqqk 356 3 Adele Adele NNP cord-104269-9r7rqqqk 356 4 Gibson Gibson NNP cord-104269-9r7rqqqk 356 5 for for IN cord-104269-9r7rqqqk 356 6 expert expert JJ cord-104269-9r7rqqqk 356 7 technical technical JJ cord-104269-9r7rqqqk 356 8 assistance assistance NN cord-104269-9r7rqqqk 356 9 and and CC cord-104269-9r7rqqqk 356 10 Albert Albert NNP cord-104269-9r7rqqqk 356 11 Tousson Tousson NNP cord-104269-9r7rqqqk 356 12 for for IN cord-104269-9r7rqqqk 356 13 helpful helpful JJ cord-104269-9r7rqqqk 356 14 discussions discussion NNS cord-104269-9r7rqqqk 356 15 and and CC cord-104269-9r7rqqqk 356 16 advice advice NN cord-104269-9r7rqqqk 356 17 on on IN cord-104269-9r7rqqqk 356 18 the the DT cord-104269-9r7rqqqk 356 19 immunofluorescence immunofluorescence NN cord-104269-9r7rqqqk 356 20 experiments experiment NNS cord-104269-9r7rqqqk 356 21 . . . cord-104269-9r7rqqqk 357 1 The the DT cord-104269-9r7rqqqk 357 2 LEP100 LEP100 NNP cord-104269-9r7rqqqk 357 3 monoclonal monoclonal JJ cord-104269-9r7rqqqk 357 4 antibody antibody NN cord-104269-9r7rqqqk 357 5 used use VBN cord-104269-9r7rqqqk 357 6 in in IN cord-104269-9r7rqqqk 357 7 these these DT cord-104269-9r7rqqqk 357 8 studies study NNS cord-104269-9r7rqqqk 357 9 was be VBD cord-104269-9r7rqqqk 357 10 obtained obtain VBN cord-104269-9r7rqqqk 357 11 from from IN cord-104269-9r7rqqqk 357 12 _SP