id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-010530-w8ir0e07	MOURA, HERCULES	Acanthamoeba healyi N. Sp. and the Isoenzyme and Immunoblot Profiles of Acanthamoeba spp., Groups 1 and 3	2007-05-01		.txt	text/plain	4382	269	62	ABSTRACT Two strains of Acanthamoeba isolated from human brain tissue and a strain of Acanthamoeba isolated from a fish were compared with 10 species of Acanthamoeba belonging to groups 1, 2 and 3 based on their isoenzyme profiles and antigenic characteristics. The isoenzymes and antigens were electrophoretically separated on polyacrylamide gradient gels, and the patterns obtained were compared after appropriate staining for particular enzymes and reactivities with homologous and heterologous rabbit anti‐Acanthamoeba antisera. This paper presents the methods used to isolate Acanthamoeba from the CNS of humans and identify them to the species level based on morphologic characteristics and isoenzyme and antigenic profiles. A small piece of the biopsied brain tissue was frozen and sent to the Centers for Disease Control, where Acanthamoeba (CDC: 1283:V013) was isolated on non-nutrient agar plates seeded with Escherichia coli and MRC human lung cell culture [ 131.	./cache/cord-010530-w8ir0e07.txt	./txt/cord-010530-w8ir0e07.txt