id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000488-x5ardo5j	Pedersen, Lasse Eggers	Porcine major histocompatibility complex (MHC) class I molecules and analysis of their peptide-binding specificities	2011-07-08		.txt	text/plain	7264	371	53	We have recently developed efficient methods to generate recombinant human MHC-I (also known as human leukocyte antigen class I, HLA-I) molecules, accompanying peptide-binding assays and predictors, and HLA tetramers for specific CTL staining and manipulation. Recombinant constructs encoding chimeric and SLA-1*0401 molecules A synthetic gene encoding a transmembrane-truncated fragment encompassing residues 1 to 275 of human HLA-A*11:01 alpha chain followed by a FXa-BSP-HAT tag (FXa = factor Xa cleavage site comprised of the amino acid sequence IEGR, BSP = biotinylation signal peptide, HAT = histidine affinity tag for purification purposes; see Online Resource 1) had previously been generated and inserted into the pET28 expression plasmid (Novagen) (Ferre et al.	./cache/cord-000488-x5ardo5j.txt	./txt/cord-000488-x5ardo5j.txt