id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-016313-n4ewq0pt	Baranyi, Lajos	Advances in Lentiviral Vector-based Cell Therapy with Mesenchymal Stem Cells	2012-09-27		.txt	text/plain	20575	824	39	The field of possible application of mesenchymal stem cells in medicine and research expanded tremendously with the advent of improved Lentiviral-vectors capable of inserting stable copies of genes of interest and expressing proteins or biologically active RNA species ad libitum, performing delicate gene editing or active gene silencing or serving as advanced drug delivery systems utilized in ex vivo cell therapy. Implantation of Lentiviral vector-transduced human bone marrow mesenchymal cells using collagen scaffolds into immunode fi cient mice resulted in ef fi cient engraftment of gene-engineered cells and provided sites for transgene-expression in vivo. Moreover, it did not alter the differentiation potential of either HSCs or MSCs. In addition, the therapeutic potential of CD133+ and MSC progenitor cells transduced ex vivo with Lentiviral vector encoding the mature form of vascular endothelial growth factor D (VEGF-D ) or the enhanced green fl uorescent protein (eGFP) marker gene achieved permanent gene expression.	./cache/cord-016313-n4ewq0pt.txt	./txt/cord-016313-n4ewq0pt.txt