id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-283339-pbgeoxdu	Jonsdottir, Hulda R.	Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures	2014-12-18		.txt	text/plain	2482	207	63	Additionally, we outline methods for immunofluorescence staining of these cultures for virus detection, characterization of cell tropism, and how to perform antiviral assays and quantify viral replication. Primary human bronchial epithelial cells cultured in an air-liquid interface (ALI) system serve as a universal platform to study human respiratory viruses [ 4 -6 ] . Additionally, we outline methods for immunofl uorescence staining of these cultures for virus detection, characterization of cell tropism and how to perform antiviral assays and quantify viral replication. The inserts need to be coated overnight with collagen type IV, necessary for development and long-term maintenance of differentiated primary airway epithelial cell cultures. 4. Apply 50 µl of HBSS to the apical side and mix with equal volume of reconstituted CellTiter-Glo enzyme solution (optimized for 24-well inserts, for other insert sizes adjust buffer amount accordingly) and incubate for 5 min at room temperature on a gyro-rocker to induce cell lysis.	./cache/cord-283339-pbgeoxdu.txt	./txt/cord-283339-pbgeoxdu.txt