id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312247-cza4qsv5	Würdinger, T	Targeting non-human coronaviruses to human cancer cells using a bispecific single-chain antibody	2005-04-21		.txt	text/plain	6601	307	47	Next, we investigated whether FIPV and fMHV could be targeted to human cancer cells by constructing a bispecific single-chain antibody directed on the one hand against the feline coronavirus spike protein – responsible for receptor binding and subsequent cell entry through virus–cell membrane fusion – and on the other hand against the human epidermal growth factor receptor (EGFR). To investigate whether scFv 23F-425 could serve as an adapter molecule for FIPV and fMHV infection via human EGFR, cultures of human cancer cell lines of different tissue origin with confirmed expression of EGFR ( Figure 4 ) were inoculated with similar amounts of FIPV or fMHV in the presence or absence of the bispecific antibody. Inoculation of Targeting non-human coronaviruses to human cancer cells T Würdinger et al FIPV and fMHV onto a number of different EGFRexpressing human cancer cell lines of various tissue origins in the presence of scFv 23F-425 resulted in infection, replication, and subsequent formation of syncytia.	./cache/cord-312247-cza4qsv5.txt	./txt/cord-312247-cza4qsv5.txt