id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003498-4ct0ywnw	Bdeir, Najat	A system for production of defective interfering particles in the absence of infectious influenza A virus	2019-03-01		.txt	text/plain	6182	326	48	Defective interfering particles (DIPs) can be generated in IAV infected cells due to errors of the viral polymerase and may suppress spread of wild type (wt) virus. Here, we investigated whether coexpression of wt segments 2-8, PB2 protein and DI-244 RNA allows for production of DIPs. Employing a novel DI-244 variant encoding mScarlet-i, we show that DI-244-based DIPs are efficiently produced in cells expressing a codon optimized version of PB2 and that these DIPs exert potent antiviral activity. Influenza A viruses A/Panama/2007/99 (H3N2) [24] and A/PR/8/34 (H1N1) produced in embryonated chicken eggs were used to assess the antiviral activity of DIPs. We further employed a recombinant vesicular stomatitis virus (VSV) that expresses a dual reporter consisting of eGFP and firefly luciferase from an additional transcription unit located between the open reading frames for the viral glycoprotein and polymerase [27] .	./cache/cord-003498-4ct0ywnw.txt	./txt/cord-003498-4ct0ywnw.txt