id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-272693-432ixb7g	Phillips, J. E.	Changes in nonstructural protein 3 are associated with attenuation in avian coronavirus infectious bronchitis virus	2011-09-10		.txt	text/plain	6255	290	56	Full-length genome sequencing of pathogenic and attenuated (for chickens) avian coronavirus infectious bronchitis virus (IBV) strains of the same serotype was conducted to identify genetic differences between the pathotypes. Analysis of the consensus full-length genome for three different IBV serotypes (Ark, GA98, and Mass41) showed that passage in embryonated eggs, to attenuate the viruses for chickens, resulted in 34.75–43.66% of all the amino acid changes occurring in nsp 3 within a virus type, whereas changes in the spike glycoprotein, thought to be the most variable protein in IBV, ranged from 5.8 to 13.4% of all changes. In this study, we analyzed the consensus full-length genome for the pathogenic and attenuated viruses of three different IBV types and showed that within a virus type, 34.75 to 43.66% of all the amino acid changes between the pathotypes occurred in nsp 3, whereas changes in spike ranged from 5.8 to 13.4% of all changes.	./cache/cord-272693-432ixb7g.txt	./txt/cord-272693-432ixb7g.txt