id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276755-ctzrgqe7	Emmott, Edward	Elucidation of the avian nucleolar proteome by quantitative proteomics using SILAC and changes in cells infected with the coronavirus infectious bronchitis virus	2010-09-08		.txt	text/plain	2488	120	35	A recent quantitative analysis of the nucleolar proteome isolated from HeLa cells infected with the nuclear replicating DNA virus adenovirus identified 351 proteins, of which 24 proteins showed at least a twofold change in abundance, compared with nucleoli from mock-infected cells [14] . For example, in one such virus, avian infectious bronchitis virus (IBV), the virally encoded nucleocapsid (N) protein localized to the nucleolus in a cell cycle-dependent manner [15] and contained appropriate targeting motifs [16] [17] [18] . Several proteins were selected from the SILAC LC-MS/ MS analysis and their abundance and localization investigated in mock and IBV-infected cells using indirect immunofluorescence confocal microscopy (as described in [18, 19] ) in order to validate the quantitative proteomic approach (Supporting Information Fig. 1 ). Ingenuity Pathway Analysis was used to investigate the data sets to group together any proteins that shared similar functions in order to build an overview of the avian nucleolar proteome (Fig. 2) and potential roles of proteins in infectious and respiratory disease.	./cache/cord-276755-ctzrgqe7.txt	./txt/cord-276755-ctzrgqe7.txt