id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300104-855iw9wi	Hennion, Ruth M.	The Preparation of Chicken Kidney Cell Cultures for Virus Propagation	2014-12-18		.txt	text/plain	1487	112	76	Chicken kidney (CK) cell cultures have historically proved useful for the assay of a number of viruses including coronaviruses. A technique for the preparation of such cell cultures, using a combination of manual and trypsin disaggregation of kidneys dissected from 2to 3-week-old birds is described. When all the kidneys required have been removed from the birds, agitate them in the beaker and discard the PBSa. Repeat this process until the wash PBSa looks clear ( see Note 6 ). 5. Dilute cell suspension in growth medium to the cell concentration required, seed culture fl asks and place in incubator until intact monolayer forms ( see Note 10 ). 6. Whilst some kidney cells may be lost in this process, it is an effective way of removing many of the red blood cells that are still present at this stage of the preparation.	./cache/cord-300104-855iw9wi.txt	./txt/cord-300104-855iw9wi.txt