id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-307304-irji8owi	Britton, Paul	Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection	2004-11-05		.txt	text/plain	4856	219	52	To demonstrate the feasibility of the method we exchanged the ectodomain of the Beaudette spike gene for the corresponding region from IBV M41 and generated two recombinant infectious bronchitis viruses (rIBVs) expressing the chimaeric S protein, validating the method as an alternative way for generating rIBVs. Avian infectious bronchitis virus (IBV), a group three member of the genus Coronavirus (order Nidovirales, family Coronaviridae), is a highly infectious pathogen of domestic fowl that replicates primarily in the respiratory tract but also in epithelial cells of the gut, kidney and oviduct (Cavanagh, 2001; Cavanagh and Naqi, 2003; Cook et al., 2001) . In an alternative strategy infectious IBV was recovered following transfection of restricted vaccinia virus DNA, containing the IBV full-length cDNA, into cells infected with recombinant fowlpox virus expressing T7 RNA polymerase (Casais et al., 2001) .	./cache/cord-307304-irji8owi.txt	./txt/cord-307304-irji8owi.txt