id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-337441-c5bxthwn	You, Jae-Hwan	Three-Dimensional Reconstruction of the Nucleolus Using Meta-Confocal Microscopy in Cells Expressing the Coronavirus Nucleoprotein	2006		.txt	text/plain	1752	100	52	We investigated the three-dimensional structure of the nucleolus and sub-nuclear bodies within cells expressing IBV and severe acute respiratory syndrome coronavirus (SARS-CoV) N protein. Live cell imaging data indicated that both EGFP and ECFP, when expressed as individual proteins, had no distinct distribution pattern and were present in both the cytoplasm and nucleus but not nucleolus (Fig. 1A) . In contrast to EGFP-tagged IBV N protein, ECFP-tagged SARS-CoV N protein localized to the cytoplasm and, in a minority of cells, to what appeared to be a nuclear body (arrowed). To investigate whether IBV N protein localized to a specific part of the nucleolus, Cos-7 cells were transfected with pEGFP-IBV-N, fixed 24 hr post-transfection, stained with PI to visualize the nucleus and nucleolus, then sectioned by confocal microscopy (Fig. 2) . META-confocal analysis and three-dimensional reconstructions of cells expressing IBV N protein revealed that N protein does not localize throughout the nucleolus and may be confined to the DFC.	./cache/cord-337441-c5bxthwn.txt	./txt/cord-337441-c5bxthwn.txt