id sid tid token lemma pos cord-349149-nqsohp9h 1 1 key key JJ cord-349149-nqsohp9h 1 2 : : : cord-349149-nqsohp9h 1 3 cord-349149-nqsohp9h cord-349149-nqsohp9h CD cord-349149-nqsohp9h 1 4 authors author NNS cord-349149-nqsohp9h 1 5 : : : cord-349149-nqsohp9h 1 6 Lounas Lounas NNP cord-349149-nqsohp9h 1 7 , , , cord-349149-nqsohp9h 1 8 A. a. NN cord-349149-nqsohp9h 1 9 ; ; : cord-349149-nqsohp9h 1 10 Oumouna Oumouna NNP cord-349149-nqsohp9h 1 11 - - HYPH cord-349149-nqsohp9h 1 12 Benachour Benachour NNP cord-349149-nqsohp9h 1 13 , , , cord-349149-nqsohp9h 1 14 K. K. NNP cord-349149-nqsohp9h 1 15 ; ; : cord-349149-nqsohp9h 1 16 Medkour Medkour NNP cord-349149-nqsohp9h 1 17 , , , cord-349149-nqsohp9h 1 18 H. H. NNP cord-349149-nqsohp9h 1 19 ; ; : cord-349149-nqsohp9h 1 20 Oumouna Oumouna NNP cord-349149-nqsohp9h 1 21 , , , cord-349149-nqsohp9h 1 22 M. M. NNP cord-349149-nqsohp9h 1 23 title title NN cord-349149-nqsohp9h 1 24 : : : cord-349149-nqsohp9h 2 1 The the DT cord-349149-nqsohp9h 2 2 first first JJ cord-349149-nqsohp9h 2 3 evidence evidence NN cord-349149-nqsohp9h 2 4 of of IN cord-349149-nqsohp9h 2 5 a a DT cord-349149-nqsohp9h 2 6 new new JJ cord-349149-nqsohp9h 2 7 genotype genotype NN cord-349149-nqsohp9h 2 8 of of IN cord-349149-nqsohp9h 2 9 nephropathogenic nephropathogenic JJ cord-349149-nqsohp9h 2 10 infectious infectious JJ cord-349149-nqsohp9h 2 11 bronchitis bronchitis NN cord-349149-nqsohp9h 2 12 virus virus NN cord-349149-nqsohp9h 2 13 circulating circulate VBG cord-349149-nqsohp9h 2 14 in in IN cord-349149-nqsohp9h 2 15 vaccinated vaccinated JJ cord-349149-nqsohp9h 2 16 and and CC cord-349149-nqsohp9h 2 17 unvaccinated unvaccinated JJ cord-349149-nqsohp9h 2 18 broiler broiler NN cord-349149-nqsohp9h 2 19 flocks flock NNS cord-349149-nqsohp9h 2 20 in in IN cord-349149-nqsohp9h 2 21 Algeria Algeria NNP cord-349149-nqsohp9h 2 22 date date NN cord-349149-nqsohp9h 2 23 : : : cord-349149-nqsohp9h 2 24 2018 2018 CD cord-349149-nqsohp9h 2 25 - - HYPH cord-349149-nqsohp9h 2 26 11 11 CD cord-349149-nqsohp9h 2 27 - - SYM cord-349149-nqsohp9h 2 28 29 29 CD cord-349149-nqsohp9h 2 29 journal journal NN cord-349149-nqsohp9h 2 30 : : : cord-349149-nqsohp9h 2 31 Vet Vet NNP cord-349149-nqsohp9h 2 32 World World NNP cord-349149-nqsohp9h 2 33 DOI doi NN cord-349149-nqsohp9h 2 34 : : : cord-349149-nqsohp9h 2 35 10.14202 10.14202 CD cord-349149-nqsohp9h 2 36 / / SYM cord-349149-nqsohp9h 2 37 vetworld.2018.1630 vetworld.2018.1630 CD cord-349149-nqsohp9h 2 38 - - HYPH cord-349149-nqsohp9h 2 39 1636 1636 CD cord-349149-nqsohp9h 2 40 sha sha NNP cord-349149-nqsohp9h 2 41 : : : cord-349149-nqsohp9h 2 42 3ba1be6ad61854f68d5d05c1bd9b046479a9dafd 3ba1be6ad61854f68d5d05c1bd9b046479a9dafd CD cord-349149-nqsohp9h 2 43 doc_id doc_id CD cord-349149-nqsohp9h 2 44 : : : cord-349149-nqsohp9h 3 1 349149 349149 CD cord-349149-nqsohp9h 3 2 cord_uid cord_uid NN cord-349149-nqsohp9h 3 3 : : : cord-349149-nqsohp9h 4 1 nqsohp9h nqsohp9h NNP cord-349149-nqsohp9h 4 2 BACKGROUND background NN cord-349149-nqsohp9h 4 3 AND and CC cord-349149-nqsohp9h 4 4 AIM aim NN cord-349149-nqsohp9h 4 5 : : : cord-349149-nqsohp9h 4 6 Avian avian JJ cord-349149-nqsohp9h 4 7 infectious infectious JJ cord-349149-nqsohp9h 4 8 bronchitis bronchitis NN cord-349149-nqsohp9h 4 9 virus virus NN cord-349149-nqsohp9h 4 10 ( ( -LRB- cord-349149-nqsohp9h 4 11 IBV IBV NNP cord-349149-nqsohp9h 4 12 ) ) -RRB- cord-349149-nqsohp9h 4 13 frequently frequently RB cord-349149-nqsohp9h 4 14 infects infect VBZ cord-349149-nqsohp9h 4 15 broilers broiler NNS cord-349149-nqsohp9h 4 16 and and CC cord-349149-nqsohp9h 4 17 is be VBZ cord-349149-nqsohp9h 4 18 responsible responsible JJ cord-349149-nqsohp9h 4 19 for for IN cord-349149-nqsohp9h 4 20 severe severe JJ cord-349149-nqsohp9h 4 21 economic economic JJ cord-349149-nqsohp9h 4 22 losses loss NNS cord-349149-nqsohp9h 4 23 to to IN cord-349149-nqsohp9h 4 24 the the DT cord-349149-nqsohp9h 4 25 poultry poultry NN cord-349149-nqsohp9h 4 26 industry industry NN cord-349149-nqsohp9h 4 27 worldwide worldwide RB cord-349149-nqsohp9h 4 28 . . . cord-349149-nqsohp9h 5 1 It -PRON- PRP cord-349149-nqsohp9h 5 2 has have VBZ cord-349149-nqsohp9h 5 3 also also RB cord-349149-nqsohp9h 5 4 been be VBN cord-349149-nqsohp9h 5 5 associated associate VBN cord-349149-nqsohp9h 5 6 with with IN cord-349149-nqsohp9h 5 7 kidney kidney NN cord-349149-nqsohp9h 5 8 damage damage NN cord-349149-nqsohp9h 5 9 in in IN cord-349149-nqsohp9h 5 10 the the DT cord-349149-nqsohp9h 5 11 broiler broiler NN cord-349149-nqsohp9h 5 12 flocks flock NNS cord-349149-nqsohp9h 5 13 . . . cord-349149-nqsohp9h 6 1 The the DT cord-349149-nqsohp9h 6 2 aim aim NN cord-349149-nqsohp9h 6 3 of of IN cord-349149-nqsohp9h 6 4 the the DT cord-349149-nqsohp9h 6 5 present present JJ cord-349149-nqsohp9h 6 6 study study NN cord-349149-nqsohp9h 6 7 is be VBZ cord-349149-nqsohp9h 6 8 to to TO cord-349149-nqsohp9h 6 9 determine determine VB cord-349149-nqsohp9h 6 10 the the DT cord-349149-nqsohp9h 6 11 presence presence NN cord-349149-nqsohp9h 6 12 of of IN cord-349149-nqsohp9h 6 13 IBV IBV NNP cord-349149-nqsohp9h 6 14 and and CC cord-349149-nqsohp9h 6 15 its -PRON- PRP$ cord-349149-nqsohp9h 6 16 possible possible JJ cord-349149-nqsohp9h 6 17 involvement involvement NN cord-349149-nqsohp9h 6 18 in in IN cord-349149-nqsohp9h 6 19 kidney kidney NN cord-349149-nqsohp9h 6 20 damage damage NN cord-349149-nqsohp9h 6 21 of of IN cord-349149-nqsohp9h 6 22 broiler broiler NN cord-349149-nqsohp9h 6 23 chicks chick NNS cord-349149-nqsohp9h 6 24 . . . cord-349149-nqsohp9h 7 1 MATERIALS materials NN cord-349149-nqsohp9h 7 2 AND and CC cord-349149-nqsohp9h 7 3 METHODS method NNS cord-349149-nqsohp9h 7 4 : : : cord-349149-nqsohp9h 7 5 14 14 CD cord-349149-nqsohp9h 7 6 clinically clinically RB cord-349149-nqsohp9h 7 7 diseased disease VBN cord-349149-nqsohp9h 7 8 broiler broiler NN cord-349149-nqsohp9h 7 9 flocks flock NNS cord-349149-nqsohp9h 7 10 from from IN cord-349149-nqsohp9h 7 11 Western western JJ cord-349149-nqsohp9h 7 12 and and CC cord-349149-nqsohp9h 7 13 Central Central NNP cord-349149-nqsohp9h 7 14 Algeria Algeria NNP cord-349149-nqsohp9h 7 15 were be VBD cord-349149-nqsohp9h 7 16 sampled sample VBN cord-349149-nqsohp9h 7 17 and and CC cord-349149-nqsohp9h 7 18 analyzed analyze VBN cord-349149-nqsohp9h 7 19 by by IN cord-349149-nqsohp9h 7 20 hemagglutination hemagglutination NN cord-349149-nqsohp9h 7 21 inhibition inhibition NN cord-349149-nqsohp9h 7 22 ( ( -LRB- cord-349149-nqsohp9h 7 23 HI HI NNP cord-349149-nqsohp9h 7 24 ) ) -RRB- cord-349149-nqsohp9h 7 25 test test VB cord-349149-nqsohp9h 7 26 and and CC cord-349149-nqsohp9h 7 27 reverse reverse VB cord-349149-nqsohp9h 7 28 transcriptase transcriptase NN cord-349149-nqsohp9h 7 29 - - HYPH cord-349149-nqsohp9h 7 30 polymerase polymerase NN cord-349149-nqsohp9h 7 31 chain chain NN cord-349149-nqsohp9h 7 32 reaction reaction NN cord-349149-nqsohp9h 7 33 ( ( -LRB- cord-349149-nqsohp9h 7 34 RT RT NNP cord-349149-nqsohp9h 7 35 - - HYPH cord-349149-nqsohp9h 7 36 PCR PCR NNP cord-349149-nqsohp9h 7 37 ) ) -RRB- cord-349149-nqsohp9h 7 38 followed follow VBN cord-349149-nqsohp9h 7 39 by by IN cord-349149-nqsohp9h 7 40 phylogenic phylogenic NN cord-349149-nqsohp9h 7 41 analysis analysis NN cord-349149-nqsohp9h 7 42 . . . cord-349149-nqsohp9h 8 1 RESULTS result NNS cord-349149-nqsohp9h 8 2 : : : cord-349149-nqsohp9h 9 1 The the DT cord-349149-nqsohp9h 9 2 QX QX NNP cord-349149-nqsohp9h 9 3 ( ( -LRB- cord-349149-nqsohp9h 9 4 100 100 CD cord-349149-nqsohp9h 9 5 % % NN cord-349149-nqsohp9h 9 6 ) ) -RRB- cord-349149-nqsohp9h 9 7 and and CC cord-349149-nqsohp9h 9 8 4/91 4/91 CD cord-349149-nqsohp9h 9 9 ( ( -LRB- cord-349149-nqsohp9h 9 10 60 60 CD cord-349149-nqsohp9h 9 11 % % NN cord-349149-nqsohp9h 9 12 ) ) -RRB- cord-349149-nqsohp9h 9 13 IBV IBV NNP cord-349149-nqsohp9h 9 14 serotypes serotype NNS cord-349149-nqsohp9h 9 15 were be VBD cord-349149-nqsohp9h 9 16 the the DT cord-349149-nqsohp9h 9 17 most most RBS cord-349149-nqsohp9h 9 18 prevalent prevalent JJ cord-349149-nqsohp9h 9 19 in in IN cord-349149-nqsohp9h 9 20 the the DT cord-349149-nqsohp9h 9 21 kidney kidney NN cord-349149-nqsohp9h 9 22 damaged damage VBD cord-349149-nqsohp9h 9 23 broilers broiler NNS cord-349149-nqsohp9h 9 24 regardless regardless RB cord-349149-nqsohp9h 9 25 of of IN cord-349149-nqsohp9h 9 26 vaccination vaccination NN cord-349149-nqsohp9h 9 27 status status NN cord-349149-nqsohp9h 9 28 . . . cord-349149-nqsohp9h 10 1 The the DT cord-349149-nqsohp9h 10 2 molecular molecular JJ cord-349149-nqsohp9h 10 3 detection detection NN cord-349149-nqsohp9h 10 4 of of IN cord-349149-nqsohp9h 10 5 avian avian JJ cord-349149-nqsohp9h 10 6 IBV IBV NNP cord-349149-nqsohp9h 10 7 by by IN cord-349149-nqsohp9h 10 8 RT RT NNP cord-349149-nqsohp9h 10 9 - - HYPH cord-349149-nqsohp9h 10 10 PCR PCR NNP cord-349149-nqsohp9h 10 11 identified identify VBD cord-349149-nqsohp9h 10 12 six six CD cord-349149-nqsohp9h 10 13 samples sample NNS cord-349149-nqsohp9h 10 14 as as IN cord-349149-nqsohp9h 10 15 positive positive JJ cord-349149-nqsohp9h 10 16 , , , cord-349149-nqsohp9h 10 17 of of IN cord-349149-nqsohp9h 10 18 which which WDT cord-349149-nqsohp9h 10 19 only only RB cord-349149-nqsohp9h 10 20 two two CD cord-349149-nqsohp9h 10 21 isolates isolate NNS cord-349149-nqsohp9h 10 22 were be VBD cord-349149-nqsohp9h 10 23 typable typable JJ cord-349149-nqsohp9h 10 24 by by IN cord-349149-nqsohp9h 10 25 sequencing sequence VBG cord-349149-nqsohp9h 10 26 . . . cord-349149-nqsohp9h 11 1 We -PRON- PRP cord-349149-nqsohp9h 11 2 identified identify VBD cord-349149-nqsohp9h 11 3 a a DT cord-349149-nqsohp9h 11 4 novel novel NN cord-349149-nqsohp9h 11 5 IBDZ13a IBDZ13a NNP cord-349149-nqsohp9h 11 6 genotype genotype NN cord-349149-nqsohp9h 11 7 which which WDT cord-349149-nqsohp9h 11 8 showed show VBD cord-349149-nqsohp9h 11 9 93 93 CD cord-349149-nqsohp9h 11 10 % % NN cord-349149-nqsohp9h 11 11 sequence sequence NN cord-349149-nqsohp9h 11 12 homology homology NN cord-349149-nqsohp9h 11 13 to to IN cord-349149-nqsohp9h 11 14 the the DT cord-349149-nqsohp9h 11 15 partial partial JJ cord-349149-nqsohp9h 11 16 - - HYPH cord-349149-nqsohp9h 11 17 S1 s1 NN cord-349149-nqsohp9h 11 18 gene gene NN cord-349149-nqsohp9h 11 19 sequence sequence NN cord-349149-nqsohp9h 11 20 of of IN cord-349149-nqsohp9h 11 21 the the DT cord-349149-nqsohp9h 11 22 IB IB NNP cord-349149-nqsohp9h 11 23 4/91 4/91 CD cord-349149-nqsohp9h 11 24 commercial commercial JJ cord-349149-nqsohp9h 11 25 vaccine vaccine NN cord-349149-nqsohp9h 11 26 strain strain NN cord-349149-nqsohp9h 11 27 . . . cord-349149-nqsohp9h 12 1 Sequencing sequencing NN cord-349149-nqsohp9h 12 2 analysis analysis NN cord-349149-nqsohp9h 12 3 characterized characterize VBD cord-349149-nqsohp9h 12 4 this this DT cord-349149-nqsohp9h 12 5 virus virus NN cord-349149-nqsohp9h 12 6 as as IN cord-349149-nqsohp9h 12 7 a a DT cord-349149-nqsohp9h 12 8 novel novel JJ cord-349149-nqsohp9h 12 9 and and CC cord-349149-nqsohp9h 12 10 divergent divergent JJ cord-349149-nqsohp9h 12 11 IB IB NNP cord-349149-nqsohp9h 12 12 4/91 4/91 CD cord-349149-nqsohp9h 12 13 field field NN cord-349149-nqsohp9h 12 14 virus virus NN cord-349149-nqsohp9h 12 15 with with IN cord-349149-nqsohp9h 12 16 eight eight CD cord-349149-nqsohp9h 12 17 amino amino NN cord-349149-nqsohp9h 12 18 acid acid NN cord-349149-nqsohp9h 12 19 substitutions substitution NNS cord-349149-nqsohp9h 12 20 that that WDT cord-349149-nqsohp9h 12 21 might may MD cord-349149-nqsohp9h 12 22 have have VB cord-349149-nqsohp9h 12 23 resulted result VBN cord-349149-nqsohp9h 12 24 in in IN cord-349149-nqsohp9h 12 25 altered alter VBN cord-349149-nqsohp9h 12 26 immunogenicity immunogenicity NN cord-349149-nqsohp9h 12 27 . . . cord-349149-nqsohp9h 13 1 CONCLUSION conclusion NN cord-349149-nqsohp9h 13 2 : : : cord-349149-nqsohp9h 14 1 The the DT cord-349149-nqsohp9h 14 2 isolation isolation NN cord-349149-nqsohp9h 14 3 of of IN cord-349149-nqsohp9h 14 4 a a DT cord-349149-nqsohp9h 14 5 new new JJ cord-349149-nqsohp9h 14 6 IBV IBV NNP cord-349149-nqsohp9h 14 7 strain strain NN cord-349149-nqsohp9h 14 8 ( ( -LRB- cord-349149-nqsohp9h 14 9 IBDZ13a ibdz13a RB cord-349149-nqsohp9h 14 10 ) ) -RRB- cord-349149-nqsohp9h 14 11 from from IN cord-349149-nqsohp9h 14 12 vaccinated vaccinate VBN cord-349149-nqsohp9h 14 13 broiler broiler NN cord-349149-nqsohp9h 14 14 flocks flock NNS cord-349149-nqsohp9h 14 15 may may MD cord-349149-nqsohp9h 14 16 explain explain VB cord-349149-nqsohp9h 14 17 the the DT cord-349149-nqsohp9h 14 18 failure failure NN cord-349149-nqsohp9h 14 19 of of IN cord-349149-nqsohp9h 14 20 the the DT cord-349149-nqsohp9h 14 21 vaccination vaccination NN cord-349149-nqsohp9h 14 22 programs program NNS cord-349149-nqsohp9h 14 23 against against IN cord-349149-nqsohp9h 14 24 IBV IBV NNP cord-349149-nqsohp9h 14 25 field field NN cord-349149-nqsohp9h 14 26 strains strain NNS cord-349149-nqsohp9h 14 27 . . . cord-349149-nqsohp9h 15 1 Combination combination NN cord-349149-nqsohp9h 15 2 of of IN cord-349149-nqsohp9h 15 3 the the DT cord-349149-nqsohp9h 15 4 HI HI NNP cord-349149-nqsohp9h 15 5 test test NN cord-349149-nqsohp9h 15 6 and and CC cord-349149-nqsohp9h 15 7 RT RT NNP cord-349149-nqsohp9h 15 8 - - HYPH cord-349149-nqsohp9h 15 9 PCR PCR NNP cord-349149-nqsohp9h 15 10 indicated indicate VBD cord-349149-nqsohp9h 15 11 that that IN cord-349149-nqsohp9h 15 12 the the DT cord-349149-nqsohp9h 15 13 nephropathogenic nephropathogenic JJ cord-349149-nqsohp9h 15 14 IB IB NNP cord-349149-nqsohp9h 15 15 outbreaks outbreak NNS cord-349149-nqsohp9h 15 16 in in IN cord-349149-nqsohp9h 15 17 broilers broiler NNS cord-349149-nqsohp9h 15 18 are be VBP cord-349149-nqsohp9h 15 19 related relate VBN cord-349149-nqsohp9h 15 20 to to IN cord-349149-nqsohp9h 15 21 this this DT cord-349149-nqsohp9h 15 22 novel novel JJ cord-349149-nqsohp9h 15 23 strain strain NN cord-349149-nqsohp9h 15 24 . . . cord-349149-nqsohp9h 16 1 Poultry poultry NN cord-349149-nqsohp9h 16 2 production production NN cord-349149-nqsohp9h 16 3 in in IN cord-349149-nqsohp9h 16 4 Algeria Algeria NNP cord-349149-nqsohp9h 16 5 faces face VBZ cord-349149-nqsohp9h 16 6 many many JJ cord-349149-nqsohp9h 16 7 zootechnical zootechnical JJ cord-349149-nqsohp9h 16 8 and and CC cord-349149-nqsohp9h 16 9 health health NN cord-349149-nqsohp9h 16 10 constraints constraint NNS cord-349149-nqsohp9h 16 11 , , , cord-349149-nqsohp9h 16 12 such such JJ cord-349149-nqsohp9h 16 13 as as IN cord-349149-nqsohp9h 16 14 viral viral JJ cord-349149-nqsohp9h 16 15 infections infection NNS cord-349149-nqsohp9h 16 16 like like IN cord-349149-nqsohp9h 16 17 avian avian JJ cord-349149-nqsohp9h 16 18 infectious infectious JJ cord-349149-nqsohp9h 16 19 bronchitis bronchitis NN cord-349149-nqsohp9h 16 20 ( ( -LRB- cord-349149-nqsohp9h 16 21 IB IB NNP cord-349149-nqsohp9h 16 22 ) ) -RRB- cord-349149-nqsohp9h 16 23 . . . cord-349149-nqsohp9h 17 1 The the DT cord-349149-nqsohp9h 17 2 avian avian JJ cord-349149-nqsohp9h 17 3 IB IB NNP cord-349149-nqsohp9h 17 4 virus virus NN cord-349149-nqsohp9h 17 5 ( ( -LRB- cord-349149-nqsohp9h 17 6 IBV IBV NNP cord-349149-nqsohp9h 17 7 ) ) -RRB- cord-349149-nqsohp9h 17 8 , , , cord-349149-nqsohp9h 17 9 a a DT cord-349149-nqsohp9h 17 10 member member NN cord-349149-nqsohp9h 17 11 of of IN cord-349149-nqsohp9h 17 12 the the DT cord-349149-nqsohp9h 17 13 Coronaviridae Coronaviridae NNP cord-349149-nqsohp9h 17 14 family family NN cord-349149-nqsohp9h 17 15 ( ( -LRB- cord-349149-nqsohp9h 17 16 order order NN cord-349149-nqsohp9h 17 17 Nidovirales Nidovirales NNP cord-349149-nqsohp9h 17 18 and and CC cord-349149-nqsohp9h 17 19 genus genus VB cord-349149-nqsohp9h 17 20 Coronavirus Coronavirus NNP cord-349149-nqsohp9h 17 21 ) ) -RRB- cord-349149-nqsohp9h 17 22 , , , cord-349149-nqsohp9h 17 23 frequently frequently RB cord-349149-nqsohp9h 17 24 infects infect VBZ cord-349149-nqsohp9h 17 25 broilers broiler NNS cord-349149-nqsohp9h 17 26 and and CC cord-349149-nqsohp9h 17 27 egg egg NN cord-349149-nqsohp9h 17 28 - - HYPH cord-349149-nqsohp9h 17 29 laying lay VBG cord-349149-nqsohp9h 17 30 hens hen NNS cord-349149-nqsohp9h 17 31 and and CC cord-349149-nqsohp9h 17 32 leads lead VBZ cord-349149-nqsohp9h 17 33 to to IN cord-349149-nqsohp9h 17 34 severe severe JJ cord-349149-nqsohp9h 17 35 economic economic JJ cord-349149-nqsohp9h 17 36 losses loss NNS cord-349149-nqsohp9h 17 37 to to IN cord-349149-nqsohp9h 17 38 the the DT cord-349149-nqsohp9h 17 39 poultry poultry NN cord-349149-nqsohp9h 17 40 industry industry NN cord-349149-nqsohp9h 17 41 [ [ -LRB- cord-349149-nqsohp9h 17 42 1 1 CD cord-349149-nqsohp9h 17 43 ] ] -RRB- cord-349149-nqsohp9h 17 44 . . . cord-349149-nqsohp9h 18 1 Since since IN cord-349149-nqsohp9h 18 2 its -PRON- PRP$ cord-349149-nqsohp9h 18 3 discovery discovery NN cord-349149-nqsohp9h 18 4 in in IN cord-349149-nqsohp9h 18 5 the the DT cord-349149-nqsohp9h 18 6 1930s 1930 NNS cord-349149-nqsohp9h 18 7 [ [ -LRB- cord-349149-nqsohp9h 18 8 2 2 CD cord-349149-nqsohp9h 18 9 ] ] -RRB- cord-349149-nqsohp9h 18 10 , , , cord-349149-nqsohp9h 18 11 the the DT cord-349149-nqsohp9h 18 12 IBV IBV NNP cord-349149-nqsohp9h 18 13 has have VBZ cord-349149-nqsohp9h 18 14 been be VBN cord-349149-nqsohp9h 18 15 identified identify VBN cord-349149-nqsohp9h 18 16 as as IN cord-349149-nqsohp9h 18 17 the the DT cord-349149-nqsohp9h 18 18 major major JJ cord-349149-nqsohp9h 18 19 cause cause NN cord-349149-nqsohp9h 18 20 of of IN cord-349149-nqsohp9h 18 21 respiratory respiratory JJ cord-349149-nqsohp9h 18 22 infections infection NNS cord-349149-nqsohp9h 18 23 and and CC cord-349149-nqsohp9h 18 24 poor poor JJ cord-349149-nqsohp9h 18 25 zootechnical zootechnical JJ cord-349149-nqsohp9h 18 26 performances performance NNS cord-349149-nqsohp9h 18 27 . . . cord-349149-nqsohp9h 19 1 Interestingly interestingly RB cord-349149-nqsohp9h 19 2 , , , cord-349149-nqsohp9h 19 3 it -PRON- PRP cord-349149-nqsohp9h 19 4 can can MD cord-349149-nqsohp9h 19 5 also also RB cord-349149-nqsohp9h 19 6 multiply multiply VB cord-349149-nqsohp9h 19 7 in in IN cord-349149-nqsohp9h 19 8 the the DT cord-349149-nqsohp9h 19 9 renal renal JJ cord-349149-nqsohp9h 19 10 tissue tissue NN cord-349149-nqsohp9h 19 11 and and CC cord-349149-nqsohp9h 19 12 cause cause VB cord-349149-nqsohp9h 19 13 nephritis nephritis NN cord-349149-nqsohp9h 19 14 [ [ -LRB- cord-349149-nqsohp9h 19 15 3 3 CD cord-349149-nqsohp9h 19 16 ] ] -RRB- cord-349149-nqsohp9h 19 17 , , , cord-349149-nqsohp9h 19 18 a a DT cord-349149-nqsohp9h 19 19 phenomenon phenomenon NN cord-349149-nqsohp9h 19 20 first first RB cord-349149-nqsohp9h 19 21 described describe VBN cord-349149-nqsohp9h 19 22 in in IN cord-349149-nqsohp9h 19 23 the the DT cord-349149-nqsohp9h 19 24 United United NNP cord-349149-nqsohp9h 19 25 States States NNP cord-349149-nqsohp9h 19 26 [ [ -LRB- cord-349149-nqsohp9h 19 27 4 4 CD cord-349149-nqsohp9h 19 28 ] ] -RRB- cord-349149-nqsohp9h 19 29 . . . cord-349149-nqsohp9h 20 1 More more RBR cord-349149-nqsohp9h 20 2 recently recently RB cord-349149-nqsohp9h 20 3 , , , cord-349149-nqsohp9h 20 4 IBV IBV NNP cord-349149-nqsohp9h 20 5 - - HYPH cord-349149-nqsohp9h 20 6 associated associate VBN cord-349149-nqsohp9h 20 7 nephritis nephritis NN cord-349149-nqsohp9h 20 8 has have VBZ cord-349149-nqsohp9h 20 9 been be VBN cord-349149-nqsohp9h 20 10 accepted accept VBN cord-349149-nqsohp9h 20 11 as as IN cord-349149-nqsohp9h 20 12 the the DT cord-349149-nqsohp9h 20 13 most most RBS cord-349149-nqsohp9h 20 14 pressing pressing JJ cord-349149-nqsohp9h 20 15 problem problem NN cord-349149-nqsohp9h 20 16 in in IN cord-349149-nqsohp9h 20 17 broiler broiler NN cord-349149-nqsohp9h 20 18 flocks flock NNS cord-349149-nqsohp9h 20 19 in in IN cord-349149-nqsohp9h 20 20 many many JJ cord-349149-nqsohp9h 20 21 countries country NNS cord-349149-nqsohp9h 20 22 [ [ -LRB- cord-349149-nqsohp9h 20 23 5 5 CD cord-349149-nqsohp9h 20 24 ] ] -RRB- cord-349149-nqsohp9h 20 25 [ [ -LRB- cord-349149-nqsohp9h 20 26 6 6 CD cord-349149-nqsohp9h 20 27 ] ] -RRB- cord-349149-nqsohp9h 20 28 [ [ -LRB- cord-349149-nqsohp9h 20 29 7 7 CD cord-349149-nqsohp9h 20 30 ] ] -RRB- cord-349149-nqsohp9h 21 1 [ [ -LRB- cord-349149-nqsohp9h 21 2 8 8 CD cord-349149-nqsohp9h 21 3 ] ] -RRB- cord-349149-nqsohp9h 21 4 [ [ -LRB- cord-349149-nqsohp9h 21 5 9 9 CD cord-349149-nqsohp9h 21 6 ] ] -RRB- cord-349149-nqsohp9h 21 7 . . . cord-349149-nqsohp9h 22 1 The the DT cord-349149-nqsohp9h 22 2 most most RBS cord-349149-nqsohp9h 22 3 effective effective JJ cord-349149-nqsohp9h 22 4 method method NN cord-349149-nqsohp9h 22 5 of of IN cord-349149-nqsohp9h 22 6 protecting protect VBG cord-349149-nqsohp9h 22 7 poultry poultry NN cord-349149-nqsohp9h 22 8 from from IN cord-349149-nqsohp9h 22 9 IBV IBV NNP cord-349149-nqsohp9h 22 10 infections infection NNS cord-349149-nqsohp9h 22 11 is be VBZ cord-349149-nqsohp9h 22 12 through through IN cord-349149-nqsohp9h 22 13 live live NN cord-349149-nqsohp9h 22 14 or or CC cord-349149-nqsohp9h 22 15 killed kill VBN cord-349149-nqsohp9h 22 16 vaccines vaccine NNS cord-349149-nqsohp9h 22 17 [ [ -LRB- cord-349149-nqsohp9h 22 18 1 1 CD cord-349149-nqsohp9h 22 19 ] ] -RRB- cord-349149-nqsohp9h 22 20 . . . cord-349149-nqsohp9h 23 1 However however RB cord-349149-nqsohp9h 23 2 , , , cord-349149-nqsohp9h 23 3 nephritis nephritis NN cord-349149-nqsohp9h 23 4 associated associate VBN cord-349149-nqsohp9h 23 5 with with IN cord-349149-nqsohp9h 23 6 infectious infectious JJ cord-349149-nqsohp9h 23 7 bronchitis bronchitis NN cord-349149-nqsohp9h 23 8 has have VBZ cord-349149-nqsohp9h 23 9 been be VBN cord-349149-nqsohp9h 23 10 observed observe VBN cord-349149-nqsohp9h 23 11 in in IN cord-349149-nqsohp9h 23 12 several several JJ cord-349149-nqsohp9h 23 13 vaccinated vaccinated JJ cord-349149-nqsohp9h 23 14 flocks flock NNS cord-349149-nqsohp9h 23 15 , , , cord-349149-nqsohp9h 23 16 suggesting suggest VBG cord-349149-nqsohp9h 23 17 that that IN cord-349149-nqsohp9h 23 18 the the DT cord-349149-nqsohp9h 23 19 current current JJ cord-349149-nqsohp9h 23 20 vaccination vaccination NN cord-349149-nqsohp9h 23 21 strategies strategy NNS cord-349149-nqsohp9h 23 22 against against IN cord-349149-nqsohp9h 23 23 IBV IBV NNP cord-349149-nqsohp9h 23 24 may may MD cord-349149-nqsohp9h 23 25 not not RB cord-349149-nqsohp9h 23 26 provide provide VB cord-349149-nqsohp9h 23 27 adequate adequate JJ cord-349149-nqsohp9h 23 28 protection protection NN cord-349149-nqsohp9h 23 29 [ [ -LRB- cord-349149-nqsohp9h 23 30 3 3 CD cord-349149-nqsohp9h 23 31 , , , cord-349149-nqsohp9h 23 32 10 10 CD cord-349149-nqsohp9h 23 33 ] ] -RRB- cord-349149-nqsohp9h 23 34 . . . cord-349149-nqsohp9h 24 1 In in IN cord-349149-nqsohp9h 24 2 fact fact NN cord-349149-nqsohp9h 24 3 , , , cord-349149-nqsohp9h 24 4 outbreaks outbreak NNS cord-349149-nqsohp9h 24 5 of of IN cord-349149-nqsohp9h 24 6 IB IB NNP cord-349149-nqsohp9h 24 7 are be VBP cord-349149-nqsohp9h 24 8 frequently frequently RB cord-349149-nqsohp9h 24 9 caused cause VBN cord-349149-nqsohp9h 24 10 due due IN cord-349149-nqsohp9h 24 11 to to IN cord-349149-nqsohp9h 24 12 the the DT cord-349149-nqsohp9h 24 13 strains strain NNS cord-349149-nqsohp9h 24 14 serologically serologically RB cord-349149-nqsohp9h 24 15 different different JJ cord-349149-nqsohp9h 24 16 from from IN cord-349149-nqsohp9h 24 17 those those DT cord-349149-nqsohp9h 24 18 used use VBN cord-349149-nqsohp9h 24 19 for for IN cord-349149-nqsohp9h 24 20 vaccination vaccination NN cord-349149-nqsohp9h 24 21 [ [ -LRB- cord-349149-nqsohp9h 24 22 11 11 CD cord-349149-nqsohp9h 24 23 , , , cord-349149-nqsohp9h 24 24 12 12 CD cord-349149-nqsohp9h 24 25 ] ] -RRB- cord-349149-nqsohp9h 24 26 . . . cord-349149-nqsohp9h 25 1 Since since IN cord-349149-nqsohp9h 25 2 its -PRON- PRP$ cord-349149-nqsohp9h 25 3 discovery discovery NN cord-349149-nqsohp9h 25 4 in in IN cord-349149-nqsohp9h 25 5 1931 1931 CD cord-349149-nqsohp9h 25 6 , , , cord-349149-nqsohp9h 25 7 a a DT cord-349149-nqsohp9h 25 8 large large JJ cord-349149-nqsohp9h 25 9 number number NN cord-349149-nqsohp9h 25 10 of of IN cord-349149-nqsohp9h 25 11 serotypes serotype NNS cord-349149-nqsohp9h 25 12 or or CC cord-349149-nqsohp9h 25 13 variants variant NNS cord-349149-nqsohp9h 25 14 of of IN cord-349149-nqsohp9h 25 15 IBV IBV NNP cord-349149-nqsohp9h 25 16 have have VBP cord-349149-nqsohp9h 25 17 emerged emerge VBN cord-349149-nqsohp9h 25 18 [ [ -LRB- cord-349149-nqsohp9h 25 19 5 5 CD cord-349149-nqsohp9h 25 20 , , , cord-349149-nqsohp9h 25 21 13 13 CD cord-349149-nqsohp9h 25 22 , , , cord-349149-nqsohp9h 25 23 14 14 CD cord-349149-nqsohp9h 25 24 ] ] -RRB- cord-349149-nqsohp9h 25 25 , , , cord-349149-nqsohp9h 25 26 and and CC cord-349149-nqsohp9h 25 27 little little JJ cord-349149-nqsohp9h 25 28 or or CC cord-349149-nqsohp9h 25 29 no no DT cord-349149-nqsohp9h 25 30 cross cross NN cord-349149-nqsohp9h 25 31 - - JJ cord-349149-nqsohp9h 25 32 protection protection JJ cord-349149-nqsohp9h 25 33 occurs occur VBZ cord-349149-nqsohp9h 25 34 between between IN cord-349149-nqsohp9h 25 35 these these DT cord-349149-nqsohp9h 25 36 serotypes serotype NNS cord-349149-nqsohp9h 25 37 [ [ -LRB- cord-349149-nqsohp9h 25 38 15 15 CD cord-349149-nqsohp9h 25 39 ] ] -RRB- cord-349149-nqsohp9h 25 40 . . . cord-349149-nqsohp9h 26 1 Therefore therefore RB cord-349149-nqsohp9h 26 2 , , , cord-349149-nqsohp9h 26 3 it -PRON- PRP cord-349149-nqsohp9h 26 4 is be VBZ cord-349149-nqsohp9h 26 5 crucial crucial JJ cord-349149-nqsohp9h 26 6 to to TO cord-349149-nqsohp9h 26 7 track track VB cord-349149-nqsohp9h 26 8 epidemic epidemic NN cord-349149-nqsohp9h 26 9 - - HYPH cord-349149-nqsohp9h 26 10 causing cause VBG cord-349149-nqsohp9h 26 11 serotypes serotype NNS cord-349149-nqsohp9h 26 12 in in IN cord-349149-nqsohp9h 26 13 each each DT cord-349149-nqsohp9h 26 14 geographic geographic JJ cord-349149-nqsohp9h 26 15 region region NN cord-349149-nqsohp9h 26 16 or or CC cord-349149-nqsohp9h 26 17 country country NN cord-349149-nqsohp9h 26 18 and and CC cord-349149-nqsohp9h 26 19 produce produce VB cord-349149-nqsohp9h 26 20 new new JJ cord-349149-nqsohp9h 26 21 vaccines vaccine NNS cord-349149-nqsohp9h 26 22 to to TO cord-349149-nqsohp9h 26 23 control control VB cord-349149-nqsohp9h 26 24 IB IB NNP cord-349149-nqsohp9h 26 25 . . . cord-349149-nqsohp9h 27 1 In in IN cord-349149-nqsohp9h 27 2 Algeria Algeria NNP cord-349149-nqsohp9h 27 3 , , , cord-349149-nqsohp9h 27 4 poultry poultry NN cord-349149-nqsohp9h 27 5 flocks flock NNS cord-349149-nqsohp9h 27 6 have have VBP cord-349149-nqsohp9h 27 7 been be VBN cord-349149-nqsohp9h 27 8 vaccinated vaccinate VBN cord-349149-nqsohp9h 27 9 against against IN cord-349149-nqsohp9h 27 10 IB IB NNP cord-349149-nqsohp9h 27 11 with with IN cord-349149-nqsohp9h 27 12 the the DT cord-349149-nqsohp9h 27 13 Massachusetts Massachusetts NNP cord-349149-nqsohp9h 27 14 ( ( -LRB- cord-349149-nqsohp9h 27 15 Mass Mass NNP cord-349149-nqsohp9h 27 16 ) ) -RRB- cord-349149-nqsohp9h 27 17 strain strain NN cord-349149-nqsohp9h 27 18 combined combine VBN cord-349149-nqsohp9h 27 19 with with IN cord-349149-nqsohp9h 27 20 the the DT cord-349149-nqsohp9h 27 21 IB IB NNP cord-349149-nqsohp9h 27 22 4/91 4/91 CD cord-349149-nqsohp9h 27 23 United United NNP cord-349149-nqsohp9h 27 24 Kingdom Kingdom NNP cord-349149-nqsohp9h 27 25 variant variant NN cord-349149-nqsohp9h 27 26 strain strain NN cord-349149-nqsohp9h 27 27 or or CC cord-349149-nqsohp9h 27 28 793 793 CD cord-349149-nqsohp9h 27 29 / / SYM cord-349149-nqsohp9h 27 30 B b NN cord-349149-nqsohp9h 27 31 since since IN cord-349149-nqsohp9h 27 32 the the DT cord-349149-nqsohp9h 27 33 past past JJ cord-349149-nqsohp9h 27 34 few few JJ cord-349149-nqsohp9h 27 35 years year NNS cord-349149-nqsohp9h 27 36 . . . cord-349149-nqsohp9h 28 1 However however RB cord-349149-nqsohp9h 28 2 , , , cord-349149-nqsohp9h 28 3 kidney kidney NN cord-349149-nqsohp9h 28 4 damage damage NN cord-349149-nqsohp9h 28 5 with with IN cord-349149-nqsohp9h 28 6 suspicion suspicion NN cord-349149-nqsohp9h 28 7 of of IN cord-349149-nqsohp9h 28 8 IB IB NNP cord-349149-nqsohp9h 28 9 has have VBZ cord-349149-nqsohp9h 28 10 also also RB cord-349149-nqsohp9h 28 11 been be VBN cord-349149-nqsohp9h 28 12 reported report VBN cord-349149-nqsohp9h 28 13 in in IN cord-349149-nqsohp9h 28 14 recent recent JJ cord-349149-nqsohp9h 28 15 years year NNS cord-349149-nqsohp9h 28 16 in in IN cord-349149-nqsohp9h 28 17 spite spite NN cord-349149-nqsohp9h 28 18 of of IN cord-349149-nqsohp9h 28 19 vaccination vaccination NN cord-349149-nqsohp9h 28 20 but but CC cord-349149-nqsohp9h 28 21 has have VBZ cord-349149-nqsohp9h 28 22 not not RB cord-349149-nqsohp9h 28 23 been be VBN cord-349149-nqsohp9h 28 24 confirmed confirm VBN cord-349149-nqsohp9h 28 25 so so RB cord-349149-nqsohp9h 28 26 far far RB cord-349149-nqsohp9h 28 27 . . . cord-349149-nqsohp9h 29 1 This this DT cord-349149-nqsohp9h 29 2 has have VBZ cord-349149-nqsohp9h 29 3 led lead VBN cord-349149-nqsohp9h 29 4 to to IN cord-349149-nqsohp9h 29 5 the the DT cord-349149-nqsohp9h 29 6 speculation speculation NN cord-349149-nqsohp9h 29 7 of of IN cord-349149-nqsohp9h 29 8 the the DT cord-349149-nqsohp9h 29 9 possible possible JJ cord-349149-nqsohp9h 29 10 emergence emergence NN cord-349149-nqsohp9h 29 11 of of IN cord-349149-nqsohp9h 29 12 variant variant JJ cord-349149-nqsohp9h 29 13 strains strain NNS cord-349149-nqsohp9h 29 14 against against IN cord-349149-nqsohp9h 29 15 which which WDT cord-349149-nqsohp9h 29 16 conventional conventional JJ cord-349149-nqsohp9h 29 17 vaccines vaccine NNS cord-349149-nqsohp9h 29 18 are be VBP cord-349149-nqsohp9h 29 19 not not RB cord-349149-nqsohp9h 29 20 completely completely RB cord-349149-nqsohp9h 29 21 effective effective JJ cord-349149-nqsohp9h 29 22 [ [ -LRB- cord-349149-nqsohp9h 29 23 16 16 CD cord-349149-nqsohp9h 29 24 ] ] -RRB- cord-349149-nqsohp9h 29 25 . . . cord-349149-nqsohp9h 30 1 The the DT cord-349149-nqsohp9h 30 2 aim aim NN cord-349149-nqsohp9h 30 3 of of IN cord-349149-nqsohp9h 30 4 this this DT cord-349149-nqsohp9h 30 5 study study NN cord-349149-nqsohp9h 30 6 was be VBD cord-349149-nqsohp9h 30 7 to to TO cord-349149-nqsohp9h 30 8 investigate investigate VB cord-349149-nqsohp9h 30 9 the the DT cord-349149-nqsohp9h 30 10 presence presence NN cord-349149-nqsohp9h 30 11 of of IN cord-349149-nqsohp9h 30 12 IBV IBV NNP cord-349149-nqsohp9h 30 13 among among IN cord-349149-nqsohp9h 30 14 Algerian algerian JJ cord-349149-nqsohp9h 30 15 broiler broiler NN cord-349149-nqsohp9h 30 16 flocks flock NNS cord-349149-nqsohp9h 30 17 and and CC cord-349149-nqsohp9h 30 18 its -PRON- PRP$ cord-349149-nqsohp9h 30 19 possible possible JJ cord-349149-nqsohp9h 30 20 involvement involvement NN cord-349149-nqsohp9h 30 21 in in IN cord-349149-nqsohp9h 30 22 broiler broiler NN cord-349149-nqsohp9h 30 23 kidney kidney NN cord-349149-nqsohp9h 30 24 damage damage NN cord-349149-nqsohp9h 30 25 . . . cord-349149-nqsohp9h 31 1 Clinically clinically RB cord-349149-nqsohp9h 31 2 diseased disease VBN cord-349149-nqsohp9h 31 3 broiler broiler NN cord-349149-nqsohp9h 31 4 flocks flock NNS cord-349149-nqsohp9h 31 5 were be VBD cord-349149-nqsohp9h 31 6 sampled sample VBN cord-349149-nqsohp9h 31 7 and and CC cord-349149-nqsohp9h 31 8 analyzed analyze VBN cord-349149-nqsohp9h 31 9 by by IN cord-349149-nqsohp9h 31 10 the the DT cord-349149-nqsohp9h 31 11 hemagglutination hemagglutination NN cord-349149-nqsohp9h 31 12 inhibition inhibition NN cord-349149-nqsohp9h 31 13 ( ( -LRB- cord-349149-nqsohp9h 31 14 HI HI NNP cord-349149-nqsohp9h 31 15 ) ) -RRB- cord-349149-nqsohp9h 31 16 test test VB cord-349149-nqsohp9h 31 17 and and CC cord-349149-nqsohp9h 31 18 reverse reverse VB cord-349149-nqsohp9h 31 19 transcriptase transcriptase NN cord-349149-nqsohp9h 31 20 - - HYPH cord-349149-nqsohp9h 31 21 polymerase polymerase NN cord-349149-nqsohp9h 31 22 chain chain NN cord-349149-nqsohp9h 31 23 reaction reaction NN cord-349149-nqsohp9h 31 24 ( ( -LRB- cord-349149-nqsohp9h 31 25 RT RT NNP cord-349149-nqsohp9h 31 26 - - HYPH cord-349149-nqsohp9h 31 27 PCR PCR NNP cord-349149-nqsohp9h 31 28 ) ) -RRB- cord-349149-nqsohp9h 31 29 followed follow VBN cord-349149-nqsohp9h 31 30 by by IN cord-349149-nqsohp9h 31 31 phylogenic phylogenic NN cord-349149-nqsohp9h 31 32 analysis analysis NN cord-349149-nqsohp9h 31 33 . . . cord-349149-nqsohp9h 32 1 All all DT cord-349149-nqsohp9h 32 2 experimental experimental JJ cord-349149-nqsohp9h 32 3 procedures procedure NNS cord-349149-nqsohp9h 32 4 were be VBD cord-349149-nqsohp9h 32 5 approved approve VBN cord-349149-nqsohp9h 32 6 by by IN cord-349149-nqsohp9h 32 7 the the DT cord-349149-nqsohp9h 32 8 Institutional Institutional NNP cord-349149-nqsohp9h 32 9 Animal Animal NNP cord-349149-nqsohp9h 32 10 Care Care NNP cord-349149-nqsohp9h 32 11 Committee Committee NNP cord-349149-nqsohp9h 32 12 of of IN cord-349149-nqsohp9h 32 13 the the DT cord-349149-nqsohp9h 32 14 National National NNP cord-349149-nqsohp9h 32 15 Administration Administration NNP cord-349149-nqsohp9h 32 16 of of IN cord-349149-nqsohp9h 32 17 the the DT cord-349149-nqsohp9h 32 18 Algerian Algerian NNP cord-349149-nqsohp9h 32 19 Higher Higher NNP cord-349149-nqsohp9h 32 20 Education Education NNP cord-349149-nqsohp9h 32 21 and and CC cord-349149-nqsohp9h 32 22 Scientific Scientific NNP cord-349149-nqsohp9h 32 23 Research Research NNP cord-349149-nqsohp9h 32 24 ( ( -LRB- cord-349149-nqsohp9h 32 25 Ethical ethical JJ cord-349149-nqsohp9h 32 26 approval approval NN cord-349149-nqsohp9h 32 27 number number NN cord-349149-nqsohp9h 32 28 : : : cord-349149-nqsohp9h 32 29 98 98 CD cord-349149-nqsohp9h 32 30 - - SYM cord-349149-nqsohp9h 32 31 11 11 CD cord-349149-nqsohp9h 32 32 , , , cord-349149-nqsohp9h 32 33 Law Law NNP cord-349149-nqsohp9h 32 34 of of IN cord-349149-nqsohp9h 32 35 August August NNP cord-349149-nqsohp9h 32 36 22 22 CD cord-349149-nqsohp9h 32 37 , , , cord-349149-nqsohp9h 32 38 1998 1998 CD cord-349149-nqsohp9h 32 39 ) ) -RRB- cord-349149-nqsohp9h 32 40 and and CC cord-349149-nqsohp9h 32 41 were be VBD cord-349149-nqsohp9h 32 42 conducted conduct VBN cord-349149-nqsohp9h 32 43 according accord VBG cord-349149-nqsohp9h 32 44 to to IN cord-349149-nqsohp9h 32 45 the the DT cord-349149-nqsohp9h 32 46 recommendations recommendation NNS cord-349149-nqsohp9h 32 47 of of IN cord-349149-nqsohp9h 32 48 the the DT cord-349149-nqsohp9h 32 49 " " `` cord-349149-nqsohp9h 32 50 Guide Guide NNP cord-349149-nqsohp9h 32 51 for for IN cord-349149-nqsohp9h 32 52 the the DT cord-349149-nqsohp9h 32 53 Care Care NNP cord-349149-nqsohp9h 32 54 and and CC cord-349149-nqsohp9h 32 55 Use Use NNP cord-349149-nqsohp9h 32 56 of of IN cord-349149-nqsohp9h 32 57 Laboratory Laboratory NNP cord-349149-nqsohp9h 32 58 Animals Animals NNPS cord-349149-nqsohp9h 32 59 . . . cord-349149-nqsohp9h 32 60 " " '' cord-349149-nqsohp9h 33 1 Fourteen fourteen CD cord-349149-nqsohp9h 33 2 commercial commercial JJ cord-349149-nqsohp9h 33 3 broiler broiler NN cord-349149-nqsohp9h 33 4 flocks flock NNS cord-349149-nqsohp9h 33 5 from from IN cord-349149-nqsohp9h 33 6 Western western JJ cord-349149-nqsohp9h 33 7 and and CC cord-349149-nqsohp9h 33 8 Central Central NNP cord-349149-nqsohp9h 33 9 Algeria Algeria NNP cord-349149-nqsohp9h 33 10 in in IN cord-349149-nqsohp9h 33 11 which which WDT cord-349149-nqsohp9h 33 12 the the DT cord-349149-nqsohp9h 33 13 chickens chicken NNS cord-349149-nqsohp9h 33 14 were be VBD cord-349149-nqsohp9h 33 15 suffering suffer VBG cord-349149-nqsohp9h 33 16 from from IN cord-349149-nqsohp9h 33 17 kidney kidney NN cord-349149-nqsohp9h 33 18 damage damage NN cord-349149-nqsohp9h 33 19 and and CC cord-349149-nqsohp9h 33 20 respiratory respiratory JJ cord-349149-nqsohp9h 33 21 disease disease NN cord-349149-nqsohp9h 33 22 were be VBD cord-349149-nqsohp9h 33 23 sampled sample VBN cord-349149-nqsohp9h 33 24 between between IN cord-349149-nqsohp9h 33 25 January January NNP cord-349149-nqsohp9h 33 26 and and CC cord-349149-nqsohp9h 33 27 December December NNP cord-349149-nqsohp9h 33 28 2012 2012 CD cord-349149-nqsohp9h 33 29 . . . cord-349149-nqsohp9h 34 1 Trachea trachea NN cord-349149-nqsohp9h 34 2 and and CC cord-349149-nqsohp9h 34 3 kidney kidney NN cord-349149-nqsohp9h 34 4 specimens specimen NNS cord-349149-nqsohp9h 34 5 were be VBD cord-349149-nqsohp9h 34 6 taken take VBN cord-349149-nqsohp9h 34 7 from from IN cord-349149-nqsohp9h 34 8 10 10 CD cord-349149-nqsohp9h 34 9 diseased diseased JJ cord-349149-nqsohp9h 34 10 chickens chicken NNS cord-349149-nqsohp9h 34 11 from from IN cord-349149-nqsohp9h 34 12 each each DT cord-349149-nqsohp9h 34 13 flock flock NN cord-349149-nqsohp9h 34 14 and and CC cord-349149-nqsohp9h 34 15 were be VBD cord-349149-nqsohp9h 34 16 directly directly RB cord-349149-nqsohp9h 34 17 applied apply VBN cord-349149-nqsohp9h 34 18 to to IN cord-349149-nqsohp9h 34 19 the the DT cord-349149-nqsohp9h 34 20 FTA FTA NNP cord-349149-nqsohp9h 34 21 ® ® POS cord-349149-nqsohp9h 34 22 cards card NNS cord-349149-nqsohp9h 34 23 ( ( -LRB- cord-349149-nqsohp9h 34 24 GE GE NNP cord-349149-nqsohp9h 34 25 Healthcare Healthcare NNP cord-349149-nqsohp9h 34 26 , , , cord-349149-nqsohp9h 34 27 UK UK NNP cord-349149-nqsohp9h 34 28 ) ) -RRB- cord-349149-nqsohp9h 34 29 during during IN cord-349149-nqsohp9h 34 30 the the DT cord-349149-nqsohp9h 34 31 acute acute JJ cord-349149-nqsohp9h 34 32 phase phase NN cord-349149-nqsohp9h 34 33 of of IN cord-349149-nqsohp9h 34 34 the the DT cord-349149-nqsohp9h 34 35 clinical clinical JJ cord-349149-nqsohp9h 34 36 outbreak outbreak NN cord-349149-nqsohp9h 34 37 . . . cord-349149-nqsohp9h 35 1 The the DT cord-349149-nqsohp9h 35 2 samples sample NNS cord-349149-nqsohp9h 35 3 were be VBD cord-349149-nqsohp9h 35 4 transported transport VBN cord-349149-nqsohp9h 35 5 and and CC cord-349149-nqsohp9h 35 6 analyzed analyze VBN cord-349149-nqsohp9h 35 7 at at IN cord-349149-nqsohp9h 35 8 the the DT cord-349149-nqsohp9h 35 9 Laboratory Laboratory NNP cord-349149-nqsohp9h 35 10 of of IN cord-349149-nqsohp9h 35 11 Virology Virology NNP cord-349149-nqsohp9h 35 12 , , , cord-349149-nqsohp9h 35 13 Instituto Instituto NNP cord-349149-nqsohp9h 35 14 Zooprofilattico Zooprofilattico NNP cord-349149-nqsohp9h 35 15 Sperimentale Sperimentale NNP cord-349149-nqsohp9h 35 16 Delle Delle NNP cord-349149-nqsohp9h 35 17 Venezie Venezie NNP cord-349149-nqsohp9h 35 18 , , , cord-349149-nqsohp9h 35 19 Italy Italy NNP cord-349149-nqsohp9h 35 20 . . . cord-349149-nqsohp9h 36 1 In in IN cord-349149-nqsohp9h 36 2 addition addition NN cord-349149-nqsohp9h 36 3 , , , cord-349149-nqsohp9h 36 4 blood blood NN cord-349149-nqsohp9h 36 5 samples sample NNS cord-349149-nqsohp9h 36 6 were be VBD cord-349149-nqsohp9h 36 7 also also RB cord-349149-nqsohp9h 36 8 collected collect VBN cord-349149-nqsohp9h 36 9 from from IN cord-349149-nqsohp9h 36 10 each each DT cord-349149-nqsohp9h 36 11 bird bird NN cord-349149-nqsohp9h 36 12 to to TO cord-349149-nqsohp9h 36 13 determine determine VB cord-349149-nqsohp9h 36 14 the the DT cord-349149-nqsohp9h 36 15 prevalence prevalence NN cord-349149-nqsohp9h 36 16 of of IN cord-349149-nqsohp9h 36 17 different different JJ cord-349149-nqsohp9h 36 18 IBV IBV NNP cord-349149-nqsohp9h 36 19 serotypes serotype NNS cord-349149-nqsohp9h 36 20 . . . cord-349149-nqsohp9h 37 1 The the DT cord-349149-nqsohp9h 37 2 serum serum NN cord-349149-nqsohp9h 37 3 was be VBD cord-349149-nqsohp9h 37 4 collected collect VBN cord-349149-nqsohp9h 37 5 from from IN cord-349149-nqsohp9h 37 6 the the DT cord-349149-nqsohp9h 37 7 samples sample NNS cord-349149-nqsohp9h 37 8 by by IN cord-349149-nqsohp9h 37 9 centrifugation centrifugation NN cord-349149-nqsohp9h 37 10 ( ( -LRB- cord-349149-nqsohp9h 37 11 2000 2000 CD cord-349149-nqsohp9h 37 12 rpm rpm NN cord-349149-nqsohp9h 37 13 / / SYM cord-349149-nqsohp9h 37 14 min min NN cord-349149-nqsohp9h 37 15 5 5 CD cord-349149-nqsohp9h 37 16 min min NNP cord-349149-nqsohp9h 37 17 ) ) -RRB- cord-349149-nqsohp9h 37 18 and and CC cord-349149-nqsohp9h 37 19 stored store VBN cord-349149-nqsohp9h 37 20 at at IN cord-349149-nqsohp9h 37 21 -20 -20 NNP cord-349149-nqsohp9h 37 22 ° ° NN cord-349149-nqsohp9h 37 23 C c NN cord-349149-nqsohp9h 37 24 for for IN cord-349149-nqsohp9h 37 25 further further JJ cord-349149-nqsohp9h 37 26 analysis analysis NN cord-349149-nqsohp9h 37 27 . . . cord-349149-nqsohp9h 38 1 HI HI NNP cord-349149-nqsohp9h 38 2 test test NN cord-349149-nqsohp9h 38 3 was be VBD cord-349149-nqsohp9h 38 4 performed perform VBN cord-349149-nqsohp9h 38 5 at at IN cord-349149-nqsohp9h 38 6 MSD MSD NNP cord-349149-nqsohp9h 38 7 International International NNP cord-349149-nqsohp9h 38 8 laboratory laboratory NN cord-349149-nqsohp9h 38 9 , , , cord-349149-nqsohp9h 38 10 Amman Amman NNP cord-349149-nqsohp9h 38 11 , , , cord-349149-nqsohp9h 38 12 Jordan Jordan NNP cord-349149-nqsohp9h 38 13 . . . cord-349149-nqsohp9h 39 1 Three three CD cord-349149-nqsohp9h 39 2 pieces piece NNS cord-349149-nqsohp9h 39 3 of of IN cord-349149-nqsohp9h 39 4 2 2 CD cord-349149-nqsohp9h 39 5 mm mm NN cord-349149-nqsohp9h 39 6 discs disc NNS cord-349149-nqsohp9h 39 7 from from IN cord-349149-nqsohp9h 39 8 the the DT cord-349149-nqsohp9h 39 9 spotted spotted JJ cord-349149-nqsohp9h 39 10 area area NN cord-349149-nqsohp9h 39 11 of of IN cord-349149-nqsohp9h 39 12 the the DT cord-349149-nqsohp9h 39 13 filters filter NNS cord-349149-nqsohp9h 39 14 were be VBD cord-349149-nqsohp9h 39 15 cut cut VBN cord-349149-nqsohp9h 39 16 by by IN cord-349149-nqsohp9h 39 17 a a DT cord-349149-nqsohp9h 39 18 sterile sterile JJ cord-349149-nqsohp9h 39 19 scissor scissor NN cord-349149-nqsohp9h 39 20 and and CC cord-349149-nqsohp9h 39 21 placed place VBN cord-349149-nqsohp9h 39 22 in in IN cord-349149-nqsohp9h 39 23 1.5 1.5 CD cord-349149-nqsohp9h 39 24 mL ml NN cord-349149-nqsohp9h 39 25 Eppendorf Eppendorf NNP cord-349149-nqsohp9h 39 26 tubes tube NNS cord-349149-nqsohp9h 39 27 . . . cord-349149-nqsohp9h 40 1 Separate separate JJ cord-349149-nqsohp9h 40 2 sterile sterile JJ cord-349149-nqsohp9h 40 3 scissors scissor NNS cord-349149-nqsohp9h 40 4 and and CC cord-349149-nqsohp9h 40 5 pincers pincer NNS cord-349149-nqsohp9h 40 6 were be VBD cord-349149-nqsohp9h 40 7 used use VBN cord-349149-nqsohp9h 40 8 for for IN cord-349149-nqsohp9h 40 9 each each DT cord-349149-nqsohp9h 40 10 sample sample NN cord-349149-nqsohp9h 40 11 to to TO cord-349149-nqsohp9h 40 12 prevent prevent VB cord-349149-nqsohp9h 40 13 cross cross NN cord-349149-nqsohp9h 40 14 - - NN cord-349149-nqsohp9h 40 15 contamination contamination NN cord-349149-nqsohp9h 40 16 . . . cord-349149-nqsohp9h 41 1 Each each DT cord-349149-nqsohp9h 41 2 disc disc NN cord-349149-nqsohp9h 41 3 was be VBD cord-349149-nqsohp9h 41 4 placed place VBN cord-349149-nqsohp9h 41 5 in in IN cord-349149-nqsohp9h 41 6 750 750 CD cord-349149-nqsohp9h 41 7 µL µl XX cord-349149-nqsohp9h 42 1 Trifast Trifast NNP cord-349149-nqsohp9h 42 2 ® ® . cord-349149-nqsohp9h 42 3 FL FL NNP cord-349149-nqsohp9h 42 4 ( ( -LRB- cord-349149-nqsohp9h 42 5 PEQLAB PEQLAB NNP cord-349149-nqsohp9h 42 6 , , , cord-349149-nqsohp9h 42 7 Germany Germany NNP cord-349149-nqsohp9h 42 8 ) ) -RRB- cord-349149-nqsohp9h 42 9 , , , cord-349149-nqsohp9h 42 10 homogenized homogenize VBN cord-349149-nqsohp9h 42 11 , , , cord-349149-nqsohp9h 42 12 and and CC cord-349149-nqsohp9h 42 13 incubated incubate VBN cord-349149-nqsohp9h 42 14 for for IN cord-349149-nqsohp9h 42 15 5 5 CD cord-349149-nqsohp9h 42 16 min min NN cord-349149-nqsohp9h 42 17 at at IN cord-349149-nqsohp9h 42 18 RT RT NNP cord-349149-nqsohp9h 42 19 . . . cord-349149-nqsohp9h 43 1 The the DT cord-349149-nqsohp9h 43 2 Trifast Trifast NNP cord-349149-nqsohp9h 43 3 FL FL NNP cord-349149-nqsohp9h 43 4 kit kit NN cord-349149-nqsohp9h 43 5 ( ( -LRB- cord-349149-nqsohp9h 43 6 Peq Peq NNP cord-349149-nqsohp9h 43 7 GOLD gold NN cord-349149-nqsohp9h 43 8 TriFast TriFast NNP cord-349149-nqsohp9h 43 9 TM TM NNP cord-349149-nqsohp9h 43 10 FL FL NNP cord-349149-nqsohp9h 43 11 , , , cord-349149-nqsohp9h 43 12 Peqlab Peqlab NNP cord-349149-nqsohp9h 43 13 ) ) -RRB- cord-349149-nqsohp9h 43 14 was be VBD cord-349149-nqsohp9h 43 15 used use VBN cord-349149-nqsohp9h 43 16 to to TO cord-349149-nqsohp9h 43 17 extract extract VB cord-349149-nqsohp9h 43 18 the the DT cord-349149-nqsohp9h 43 19 RNA RNA NNP cord-349149-nqsohp9h 43 20 from from IN cord-349149-nqsohp9h 43 21 the the DT cord-349149-nqsohp9h 43 22 FTA FTA NNP cord-349149-nqsohp9h 43 23 ® ® POS cord-349149-nqsohp9h 43 24 paper paper NN cord-349149-nqsohp9h 43 25 according accord VBG cord-349149-nqsohp9h 43 26 to to IN cord-349149-nqsohp9h 43 27 the the DT cord-349149-nqsohp9h 43 28 manufacturer manufacturer NN cord-349149-nqsohp9h 43 29 's 's POS cord-349149-nqsohp9h 43 30 instructions instruction NNS cord-349149-nqsohp9h 43 31 ( ( -LRB- cord-349149-nqsohp9h 43 32 PEQLAB PEQLAB NNP cord-349149-nqsohp9h 43 33 Biotechnologie Biotechnologie NNP cord-349149-nqsohp9h 43 34 GmbH GmbH NNP cord-349149-nqsohp9h 43 35 , , , cord-349149-nqsohp9h 43 36 Erlangen Erlangen NNP cord-349149-nqsohp9h 43 37 , , , cord-349149-nqsohp9h 43 38 Germany Germany NNP cord-349149-nqsohp9h 43 39 ) ) -RRB- cord-349149-nqsohp9h 43 40 . . . cord-349149-nqsohp9h 44 1 RNA RNA NNP cord-349149-nqsohp9h 44 2 purity purity NN cord-349149-nqsohp9h 44 3 and and CC cord-349149-nqsohp9h 44 4 concentration concentration NN cord-349149-nqsohp9h 44 5 were be VBD cord-349149-nqsohp9h 44 6 determined determine VBN cord-349149-nqsohp9h 44 7 by by IN cord-349149-nqsohp9h 44 8 NanoDrop NanoDrop NNP cord-349149-nqsohp9h 45 1 ® ® VB cord-349149-nqsohp9h 45 2 ND-1000 ND-1000 . cord-349149-nqsohp9h 46 1 ( ( -LRB- cord-349149-nqsohp9h 46 2 Peqlab Peqlab NNP cord-349149-nqsohp9h 46 3 Biotechnologie Biotechnologie NNP cord-349149-nqsohp9h 46 4 GmbH GmbH NNP cord-349149-nqsohp9h 46 5 , , , cord-349149-nqsohp9h 46 6 Erlangen Erlangen NNP cord-349149-nqsohp9h 46 7 , , , cord-349149-nqsohp9h 46 8 Germany Germany NNP cord-349149-nqsohp9h 46 9 ) ) -RRB- cord-349149-nqsohp9h 46 10 . . . cord-349149-nqsohp9h 47 1 Reverse reverse JJ cord-349149-nqsohp9h 47 2 transcription transcription NN cord-349149-nqsohp9h 47 3 was be VBD cord-349149-nqsohp9h 47 4 performed perform VBN cord-349149-nqsohp9h 47 5 using use VBG cord-349149-nqsohp9h 47 6 the the DT cord-349149-nqsohp9h 47 7 ImProm ImProm NNP cord-349149-nqsohp9h 47 8 - - HYPH cord-349149-nqsohp9h 47 9 II II NNP cord-349149-nqsohp9h 47 10 TM TM NNP cord-349149-nqsohp9h 47 11 Reverse Reverse NNP cord-349149-nqsohp9h 47 12 Transcription Transcription NNP cord-349149-nqsohp9h 47 13 System System NNP cord-349149-nqsohp9h 47 14 ( ( -LRB- cord-349149-nqsohp9h 47 15 Promega Promega NNP cord-349149-nqsohp9h 47 16 , , , cord-349149-nqsohp9h 47 17 Madison Madison NNP cord-349149-nqsohp9h 47 18 , , , cord-349149-nqsohp9h 47 19 WI WI NNP cord-349149-nqsohp9h 47 20 ) ) -RRB- cord-349149-nqsohp9h 47 21 with with IN cord-349149-nqsohp9h 47 22 random random JJ cord-349149-nqsohp9h 47 23 primers primer NNS cord-349149-nqsohp9h 47 24 according accord VBG cord-349149-nqsohp9h 47 25 to to IN cord-349149-nqsohp9h 47 26 the the DT cord-349149-nqsohp9h 47 27 manufacturer manufacturer NN cord-349149-nqsohp9h 47 28 's 's POS cord-349149-nqsohp9h 47 29 instructions instruction NNS cord-349149-nqsohp9h 47 30 . . . cord-349149-nqsohp9h 48 1 Briefly briefly RB cord-349149-nqsohp9h 48 2 , , , cord-349149-nqsohp9h 48 3 0.1 0.1 CD cord-349149-nqsohp9h 48 4 µM µm CD cord-349149-nqsohp9h 48 5 lyophilized lyophilize VBN cord-349149-nqsohp9h 48 6 oligonucleotides oligonucleotide NNS cord-349149-nqsohp9h 48 7 provided provide VBN cord-349149-nqsohp9h 48 8 with with IN cord-349149-nqsohp9h 48 9 the the DT cord-349149-nqsohp9h 48 10 kit kit NN cord-349149-nqsohp9h 48 11 were be VBD cord-349149-nqsohp9h 48 12 dissolved dissolve VBN cord-349149-nqsohp9h 48 13 in in IN cord-349149-nqsohp9h 48 14 1 1 CD cord-349149-nqsohp9h 48 15 mL ml NN cord-349149-nqsohp9h 48 16 RNase rnase NN cord-349149-nqsohp9h 48 17 - - HYPH cord-349149-nqsohp9h 48 18 free free JJ cord-349149-nqsohp9h 48 19 water water NN cord-349149-nqsohp9h 48 20 , , , cord-349149-nqsohp9h 48 21 and and CC cord-349149-nqsohp9h 48 22 1 1 CD cord-349149-nqsohp9h 48 23 µL µl CD cord-349149-nqsohp9h 48 24 of of IN cord-349149-nqsohp9h 48 25 the the DT cord-349149-nqsohp9h 48 26 random random JJ cord-349149-nqsohp9h 48 27 primer primer NN cord-349149-nqsohp9h 48 28 solution solution NN cord-349149-nqsohp9h 48 29 was be VBD cord-349149-nqsohp9h 48 30 mixed mix VBN cord-349149-nqsohp9h 48 31 with with IN cord-349149-nqsohp9h 48 32 4 4 CD cord-349149-nqsohp9h 48 33 µL µl CD cord-349149-nqsohp9h 48 34 viral viral JJ cord-349149-nqsohp9h 48 35 RNA RNA NNP cord-349149-nqsohp9h 48 36 and and CC cord-349149-nqsohp9h 48 37 incubated incubate VBN cord-349149-nqsohp9h 48 38 at at IN cord-349149-nqsohp9h 48 39 70 70 CD cord-349149-nqsohp9h 48 40 ° ° NN cord-349149-nqsohp9h 48 41 C c NN cord-349149-nqsohp9h 48 42 for for IN cord-349149-nqsohp9h 48 43 5 5 CD cord-349149-nqsohp9h 48 44 min min NN cord-349149-nqsohp9h 48 45 . . . cord-349149-nqsohp9h 49 1 After after IN cord-349149-nqsohp9h 49 2 adding add VBG cord-349149-nqsohp9h 49 3 Buffer buffer NN cord-349149-nqsohp9h 49 4 5× 5× CD cord-349149-nqsohp9h 49 5 ( ( -LRB- cord-349149-nqsohp9h 49 6 56 56 CD cord-349149-nqsohp9h 49 7 µL µl CD cord-349149-nqsohp9h 49 8 ) ) -RRB- cord-349149-nqsohp9h 49 9 , , , cord-349149-nqsohp9h 49 10 MgCl MgCl NNP cord-349149-nqsohp9h 49 11 2 2 CD cord-349149-nqsohp9h 49 12 ( ( -LRB- cord-349149-nqsohp9h 49 13 67.2 67.2 CD cord-349149-nqsohp9h 49 14 µL µl XX cord-349149-nqsohp9h 49 15 ) ) -RRB- cord-349149-nqsohp9h 49 16 , , , cord-349149-nqsohp9h 49 17 dNTP dNTP NNP cord-349149-nqsohp9h 49 18 ( ( -LRB- cord-349149-nqsohp9h 49 19 14 14 CD cord-349149-nqsohp9h 49 20 µL µL NNP cord-349149-nqsohp9h 49 21 ) ) -RRB- cord-349149-nqsohp9h 49 22 , , , cord-349149-nqsohp9h 49 23 RNAsin RNAsin NNP cord-349149-nqsohp9h 49 24 ( ( -LRB- cord-349149-nqsohp9h 49 25 7 7 CD cord-349149-nqsohp9h 49 26 µL µL NNP cord-349149-nqsohp9h 49 27 ) ) -RRB- cord-349149-nqsohp9h 49 28 , , , cord-349149-nqsohp9h 49 29 ImProm ImProm NNP cord-349149-nqsohp9h 49 30 II II NNP cord-349149-nqsohp9h 49 31 ( ( -LRB- cord-349149-nqsohp9h 49 32 14 14 CD cord-349149-nqsohp9h 49 33 µL µL NNP cord-349149-nqsohp9h 49 34 ) ) -RRB- cord-349149-nqsohp9h 49 35 , , , cord-349149-nqsohp9h 49 36 and and CC cord-349149-nqsohp9h 49 37 H h NN cord-349149-nqsohp9h 49 38 2 2 CD cord-349149-nqsohp9h 49 39 O o NN cord-349149-nqsohp9h 49 40 ( ( -LRB- cord-349149-nqsohp9h 49 41 51.8 51.8 CD cord-349149-nqsohp9h 49 42 µL µl CD cord-349149-nqsohp9h 49 43 ) ) -RRB- cord-349149-nqsohp9h 49 44 , , , cord-349149-nqsohp9h 49 45 the the DT cord-349149-nqsohp9h 49 46 mixture mixture NN cord-349149-nqsohp9h 49 47 was be VBD cord-349149-nqsohp9h 49 48 incubated incubate VBN cord-349149-nqsohp9h 49 49 at at IN cord-349149-nqsohp9h 49 50 25 25 CD cord-349149-nqsohp9h 49 51 ° ° NN cord-349149-nqsohp9h 49 52 C c NN cord-349149-nqsohp9h 49 53 for for IN cord-349149-nqsohp9h 49 54 5 5 CD cord-349149-nqsohp9h 49 55 min min NN cord-349149-nqsohp9h 49 56 followed follow VBN cord-349149-nqsohp9h 49 57 by by IN cord-349149-nqsohp9h 49 58 55 55 CD cord-349149-nqsohp9h 49 59 ° ° NN cord-349149-nqsohp9h 49 60 C c NN cord-349149-nqsohp9h 49 61 for for IN cord-349149-nqsohp9h 49 62 60 60 CD cord-349149-nqsohp9h 49 63 min min NN cord-349149-nqsohp9h 49 64 . . . cord-349149-nqsohp9h 50 1 The the DT cord-349149-nqsohp9h 50 2 reaction reaction NN cord-349149-nqsohp9h 50 3 was be VBD cord-349149-nqsohp9h 50 4 terminated terminate VBN cord-349149-nqsohp9h 50 5 by by IN cord-349149-nqsohp9h 50 6 heating heat VBG cord-349149-nqsohp9h 50 7 at at IN cord-349149-nqsohp9h 50 8 70 70 CD cord-349149-nqsohp9h 50 9 ° ° NN cord-349149-nqsohp9h 50 10 C c NN cord-349149-nqsohp9h 50 11 for for IN cord-349149-nqsohp9h 50 12 15 15 CD cord-349149-nqsohp9h 50 13 min min NN cord-349149-nqsohp9h 50 14 and and CC cord-349149-nqsohp9h 50 15 cooling cool VBG cord-349149-nqsohp9h 50 16 on on IN cord-349149-nqsohp9h 50 17 ice ice NN cord-349149-nqsohp9h 50 18 . . . cord-349149-nqsohp9h 51 1 The the DT cord-349149-nqsohp9h 51 2 primer primer NN cord-349149-nqsohp9h 51 3 sets set NNS cord-349149-nqsohp9h 51 4 ( ( -LRB- cord-349149-nqsohp9h 51 5 Oligo5 Oligo5 NNP cord-349149-nqsohp9h 51 6 [ [ -LRB- cord-349149-nqsohp9h 51 7 forward forward RB cord-349149-nqsohp9h 51 8 ] ] -RRB- cord-349149-nqsohp9h 51 9 : : : cord-349149-nqsohp9h 52 1 5'-TGA 5'-tga XX cord-349149-nqsohp9h 53 1 AAA AAA NNP cord-349149-nqsohp9h 53 2 CTG CTG NNP cord-349149-nqsohp9h 53 3 AAC aac NN cord-349149-nqsohp9h 53 4 AAA AAA NNP cord-349149-nqsohp9h 53 5 AGA AGA NNP cord-349149-nqsohp9h 53 6 CA-3 CA-3 NNP cord-349149-nqsohp9h 53 7 ' ' '' cord-349149-nqsohp9h 53 8 and and CC cord-349149-nqsohp9h 53 9 CK2 CK2 NNP cord-349149-nqsohp9h 53 10 [ [ -LRB- cord-349149-nqsohp9h 53 11 reverse reverse NN cord-349149-nqsohp9h 53 12 ] ] -RRB- cord-349149-nqsohp9h 53 13 : : : cord-349149-nqsohp9h 54 1 5'-CTC 5'-ctc CD cord-349149-nqsohp9h 55 1 GAA GAA NNP cord-349149-nqsohp9h 55 2 TTC TTC NNP cord-349149-nqsohp9h 55 3 CNG CNG NNP cord-349149-nqsohp9h 55 4 TRT TRT NNP cord-349149-nqsohp9h 55 5 TRT TRT NNP cord-349149-nqsohp9h 55 6 AYT AYT NNP cord-349149-nqsohp9h 55 7 GRC GRC NNP cord-349149-nqsohp9h 55 8 A-3 A-3 NNP cord-349149-nqsohp9h 55 9 ' ' '' cord-349149-nqsohp9h 55 10 ) ) -RRB- cord-349149-nqsohp9h 55 11 were be VBD cord-349149-nqsohp9h 55 12 designed design VBN cord-349149-nqsohp9h 55 13 for for IN cord-349149-nqsohp9h 55 14 the the DT cord-349149-nqsohp9h 55 15 IBV IBV NNP cord-349149-nqsohp9h 55 16 S1 S1 NNP cord-349149-nqsohp9h 55 17 gene gene NN cord-349149-nqsohp9h 55 18 , , , cord-349149-nqsohp9h 55 19 which which WDT cord-349149-nqsohp9h 55 20 contains contain VBZ cord-349149-nqsohp9h 55 21 the the DT cord-349149-nqsohp9h 55 22 hypervariable hypervariable JJ cord-349149-nqsohp9h 55 23 region region NN cord-349149-nqsohp9h 55 24 1 1 CD cord-349149-nqsohp9h 55 25 and and CC cord-349149-nqsohp9h 55 26 most most JJS cord-349149-nqsohp9h 55 27 of of IN cord-349149-nqsohp9h 55 28 the the DT cord-349149-nqsohp9h 55 29 hypervariable hypervariable JJ cord-349149-nqsohp9h 55 30 region region NN cord-349149-nqsohp9h 55 31 2 2 CD cord-349149-nqsohp9h 55 32 [ [ -LRB- cord-349149-nqsohp9h 55 33 17 17 CD cord-349149-nqsohp9h 55 34 ] ] -RRB- cord-349149-nqsohp9h 55 35 . . . cord-349149-nqsohp9h 56 1 The the DT cord-349149-nqsohp9h 56 2 S1 s1 NN cord-349149-nqsohp9h 56 3 gene gene NN cord-349149-nqsohp9h 56 4 was be VBD cord-349149-nqsohp9h 56 5 amplified amplify VBN cord-349149-nqsohp9h 56 6 with with IN cord-349149-nqsohp9h 56 7 the the DT cord-349149-nqsohp9h 56 8 Superscript superscript NN cord-349149-nqsohp9h 56 9 ® ® NN cord-349149-nqsohp9h 56 10 III III NNP cord-349149-nqsohp9h 57 1 One one CD cord-349149-nqsohp9h 57 2 - - HYPH cord-349149-nqsohp9h 57 3 Step Step NNP cord-349149-nqsohp9h 57 4 RT RT NNP cord-349149-nqsohp9h 57 5 - - HYPH cord-349149-nqsohp9h 57 6 PCR PCR NNP cord-349149-nqsohp9h 57 7 Platinum Platinum NNP cord-349149-nqsohp9h 58 1 ® ® , cord-349149-nqsohp9h 58 2 Taq Taq NNP cord-349149-nqsohp9h 58 3 HiFi HiFi NNP cord-349149-nqsohp9h 58 4 ( ( -LRB- cord-349149-nqsohp9h 58 5 Invitrogen Invitrogen NNP cord-349149-nqsohp9h 58 6 TM TM NNP cord-349149-nqsohp9h 58 7 Life Life NNP cord-349149-nqsohp9h 58 8 Technologies Technologies NNP cord-349149-nqsohp9h 58 9 TM TM NNP cord-349149-nqsohp9h 58 10 , , , cord-349149-nqsohp9h 58 11 Carlsbad Carlsbad NNP cord-349149-nqsohp9h 58 12 , , , cord-349149-nqsohp9h 58 13 CA CA NNP cord-349149-nqsohp9h 58 14 ) ) -RRB- cord-349149-nqsohp9h 58 15 , , , cord-349149-nqsohp9h 58 16 which which WDT cord-349149-nqsohp9h 58 17 generated generate VBD cord-349149-nqsohp9h 58 18 a a DT cord-349149-nqsohp9h 58 19 PCR PCR NNP cord-349149-nqsohp9h 58 20 product product NN cord-349149-nqsohp9h 58 21 of of IN cord-349149-nqsohp9h 58 22 344 344 CD cord-349149-nqsohp9h 58 23 bp bp NN cord-349149-nqsohp9h 58 24 . . . cord-349149-nqsohp9h 59 1 For for IN cord-349149-nqsohp9h 59 2 the the DT cord-349149-nqsohp9h 59 3 PCR PCR NNP cord-349149-nqsohp9h 59 4 reaction reaction NN cord-349149-nqsohp9h 59 5 , , , cord-349149-nqsohp9h 59 6 the the DT cord-349149-nqsohp9h 59 7 following follow VBG cord-349149-nqsohp9h 59 8 reaction reaction NN cord-349149-nqsohp9h 59 9 mix mix NN cord-349149-nqsohp9h 59 10 was be VBD cord-349149-nqsohp9h 59 11 made make VBN cord-349149-nqsohp9h 59 12 : : : cord-349149-nqsohp9h 60 1 35 35 CD cord-349149-nqsohp9h 60 2 µL µl XX cord-349149-nqsohp9h 61 1 Buffer buffer NN cord-349149-nqsohp9h 61 2 10× 10× CD cord-349149-nqsohp9h 61 3 , , , cord-349149-nqsohp9h 61 4 14 14 CD cord-349149-nqsohp9h 61 5 µL µl XX cord-349149-nqsohp9h 62 1 dNTP dNTP NNP cord-349149-nqsohp9h 62 2 , , , cord-349149-nqsohp9h 62 3 14 14 CD cord-349149-nqsohp9h 62 4 µL µl XX cord-349149-nqsohp9h 63 1 CK2 CK2 NNP cord-349149-nqsohp9h 63 2 , , , cord-349149-nqsohp9h 63 3 14 14 CD cord-349149-nqsohp9h 63 4 µL µL NNP cord-349149-nqsohp9h 63 5 Oligo5 Oligo5 NNP cord-349149-nqsohp9h 63 6 , , , cord-349149-nqsohp9h 64 1 243.25 243.25 CD cord-349149-nqsohp9h 64 2 µL µl XX cord-349149-nqsohp9h 65 1 H h NN cord-349149-nqsohp9h 66 1 2 2 LS cord-349149-nqsohp9h 66 2 O o NN cord-349149-nqsohp9h 66 3 , , , cord-349149-nqsohp9h 66 4 1.75 1.75 CD cord-349149-nqsohp9h 66 5 µL µl XX cord-349149-nqsohp9h 67 1 Taq taq NN cord-349149-nqsohp9h 67 2 polymerase polymerase NN cord-349149-nqsohp9h 67 3 ( ( -LRB- cord-349149-nqsohp9h 67 4 Taq Taq NNP cord-349149-nqsohp9h 67 5 HIFI HIFI NNP cord-349149-nqsohp9h 67 6 , , , cord-349149-nqsohp9h 67 7 Invitrogen Invitrogen NNP cord-349149-nqsohp9h 67 8 TM TM NNP cord-349149-nqsohp9h 67 9 Life Life NNP cord-349149-nqsohp9h 67 10 Technologies Technologies NNP cord-349149-nqsohp9h 67 11 TM TM NNP cord-349149-nqsohp9h 67 12 , , , cord-349149-nqsohp9h 67 13 Carlsbad Carlsbad NNP cord-349149-nqsohp9h 67 14 , , , cord-349149-nqsohp9h 67 15 CA CA NNP cord-349149-nqsohp9h 67 16 ) ) -RRB- cord-349149-nqsohp9h 67 17 , , , cord-349149-nqsohp9h 67 18 and and CC cord-349149-nqsohp9h 67 19 2 2 CD cord-349149-nqsohp9h 67 20 µL µL NNP cord-349149-nqsohp9h 67 21 cDNA cdna NN cord-349149-nqsohp9h 67 22 to to IN cord-349149-nqsohp9h 67 23 a a DT cord-349149-nqsohp9h 67 24 final final JJ cord-349149-nqsohp9h 67 25 volume volume NN cord-349149-nqsohp9h 67 26 of of IN cord-349149-nqsohp9h 67 27 25 25 CD cord-349149-nqsohp9h 67 28 µL. µl. NN cord-349149-nqsohp9h 67 29 The the DT cord-349149-nqsohp9h 67 30 PCR PCR NNP cord-349149-nqsohp9h 67 31 parameters parameter NNS cord-349149-nqsohp9h 67 32 were be VBD cord-349149-nqsohp9h 67 33 as as IN cord-349149-nqsohp9h 67 34 follows follow VBZ cord-349149-nqsohp9h 67 35 : : : cord-349149-nqsohp9h 67 36 45 45 CD cord-349149-nqsohp9h 67 37 cycles cycle NNS cord-349149-nqsohp9h 67 38 of of IN cord-349149-nqsohp9h 67 39 denaturation denaturation NN cord-349149-nqsohp9h 67 40 ( ( -LRB- cord-349149-nqsohp9h 67 41 94 94 CD cord-349149-nqsohp9h 67 42 ° ° NN cord-349149-nqsohp9h 67 43 C c NN cord-349149-nqsohp9h 67 44 , , , cord-349149-nqsohp9h 67 45 20 20 CD cord-349149-nqsohp9h 67 46 s s NNS cord-349149-nqsohp9h 67 47 ) ) -RRB- cord-349149-nqsohp9h 67 48 , , , cord-349149-nqsohp9h 67 49 annealing anneal VBG cord-349149-nqsohp9h 67 50 ( ( -LRB- cord-349149-nqsohp9h 67 51 51 51 CD cord-349149-nqsohp9h 67 52 ° ° NN cord-349149-nqsohp9h 67 53 C c NN cord-349149-nqsohp9h 67 54 , , , cord-349149-nqsohp9h 67 55 1 1 CD cord-349149-nqsohp9h 67 56 min min NNP cord-349149-nqsohp9h 67 57 ) ) -RRB- cord-349149-nqsohp9h 67 58 , , , cord-349149-nqsohp9h 67 59 and and CC cord-349149-nqsohp9h 67 60 polymerization polymerization NN cord-349149-nqsohp9h 67 61 ( ( -LRB- cord-349149-nqsohp9h 67 62 72 72 CD cord-349149-nqsohp9h 67 63 ° ° NN cord-349149-nqsohp9h 67 64 C c NN cord-349149-nqsohp9h 67 65 , , , cord-349149-nqsohp9h 67 66 1 1 CD cord-349149-nqsohp9h 67 67 min min NN cord-349149-nqsohp9h 67 68 ) ) -RRB- cord-349149-nqsohp9h 67 69 . . . cord-349149-nqsohp9h 68 1 The the DT cord-349149-nqsohp9h 68 2 pre pre JJ cord-349149-nqsohp9h 68 3 - - JJ cord-349149-nqsohp9h 68 4 denaturation denaturation JJ cord-349149-nqsohp9h 68 5 step step NN cord-349149-nqsohp9h 68 6 was be VBD cord-349149-nqsohp9h 68 7 done do VBN cord-349149-nqsohp9h 68 8 at at IN cord-349149-nqsohp9h 68 9 94 94 CD cord-349149-nqsohp9h 68 10 ° ° NN cord-349149-nqsohp9h 68 11 C c NN cord-349149-nqsohp9h 68 12 for for IN cord-349149-nqsohp9h 68 13 5 5 CD cord-349149-nqsohp9h 68 14 min min NN cord-349149-nqsohp9h 68 15 and and CC cord-349149-nqsohp9h 68 16 post post JJ cord-349149-nqsohp9h 68 17 - - JJ cord-349149-nqsohp9h 68 18 polymerization polymerization JJ cord-349149-nqsohp9h 68 19 step step NN cord-349149-nqsohp9h 68 20 at at IN cord-349149-nqsohp9h 68 21 70 70 CD cord-349149-nqsohp9h 68 22 ° ° NN cord-349149-nqsohp9h 68 23 C c NN cord-349149-nqsohp9h 68 24 for for IN cord-349149-nqsohp9h 68 25 10 10 CD cord-349149-nqsohp9h 68 26 min min NN cord-349149-nqsohp9h 68 27 . . . cord-349149-nqsohp9h 69 1 A a DT cord-349149-nqsohp9h 69 2 20 20 CD cord-349149-nqsohp9h 69 3 µL µl XX cord-349149-nqsohp9h 70 1 aliquot aliquot NN cord-349149-nqsohp9h 70 2 of of IN cord-349149-nqsohp9h 70 3 each each DT cord-349149-nqsohp9h 70 4 PCR PCR NNP cord-349149-nqsohp9h 70 5 product product NN cord-349149-nqsohp9h 70 6 was be VBD cord-349149-nqsohp9h 70 7 electrophoresed electrophorese VBN cord-349149-nqsohp9h 70 8 at at IN cord-349149-nqsohp9h 70 9 120 120 CD cord-349149-nqsohp9h 70 10 V v NN cord-349149-nqsohp9h 70 11 for for IN cord-349149-nqsohp9h 70 12 40 40 CD cord-349149-nqsohp9h 70 13 min min NNS cord-349149-nqsohp9h 70 14 in in IN cord-349149-nqsohp9h 70 15 1.5 1.5 CD cord-349149-nqsohp9h 70 16 % % NN cord-349149-nqsohp9h 70 17 agarose agarose NN cord-349149-nqsohp9h 70 18 gel gel NN cord-349149-nqsohp9h 70 19 , , , cord-349149-nqsohp9h 70 20 which which WDT cord-349149-nqsohp9h 70 21 was be VBD cord-349149-nqsohp9h 70 22 then then RB cord-349149-nqsohp9h 70 23 stained stain VBN cord-349149-nqsohp9h 70 24 with with IN cord-349149-nqsohp9h 70 25 GelRed GelRed NNP cord-349149-nqsohp9h 70 26 TM TM NNP cord-349149-nqsohp9h 70 27 ( ( -LRB- cord-349149-nqsohp9h 70 28 Genaxxon Genaxxon NNP cord-349149-nqsohp9h 70 29 Bioscience Bioscience NNP cord-349149-nqsohp9h 70 30 , , , cord-349149-nqsohp9h 70 31 Ulm Ulm NNP cord-349149-nqsohp9h 70 32 , , , cord-349149-nqsohp9h 70 33 Germany Germany NNP cord-349149-nqsohp9h 70 34 , , , cord-349149-nqsohp9h 70 35 GmbH GmbH NNP cord-349149-nqsohp9h 70 36 ) ) -RRB- cord-349149-nqsohp9h 70 37 . . . cord-349149-nqsohp9h 71 1 The the DT cord-349149-nqsohp9h 71 2 amplified amplify VBN cord-349149-nqsohp9h 71 3 cDNA cdna NN cord-349149-nqsohp9h 71 4 was be VBD cord-349149-nqsohp9h 71 5 purified purify VBN cord-349149-nqsohp9h 71 6 using use VBG cord-349149-nqsohp9h 71 7 the the DT cord-349149-nqsohp9h 71 8 NucleoSpin NucleoSpin NNP cord-349149-nqsohp9h 72 1 ® ® VB cord-349149-nqsohp9h 72 2 ( ( -LRB- cord-349149-nqsohp9h 72 3 MACHEREY MACHEREY NNP cord-349149-nqsohp9h 72 4 - - HYPH cord-349149-nqsohp9h 72 5 NAGEL NAGEL NNP cord-349149-nqsohp9h 72 6 GmbH GmbH NNP cord-349149-nqsohp9h 72 7 , , , cord-349149-nqsohp9h 72 8 Germany Germany NNP cord-349149-nqsohp9h 72 9 ) ) -RRB- cord-349149-nqsohp9h 72 10 Gel Gel NNP cord-349149-nqsohp9h 72 11 and and CC cord-349149-nqsohp9h 72 12 PCR PCR NNP cord-349149-nqsohp9h 72 13 Clean Clean NNP cord-349149-nqsohp9h 72 14 - - HYPH cord-349149-nqsohp9h 72 15 up up RP cord-349149-nqsohp9h 72 16 kit kit NN cord-349149-nqsohp9h 72 17 according accord VBG cord-349149-nqsohp9h 72 18 to to IN cord-349149-nqsohp9h 72 19 the the DT cord-349149-nqsohp9h 72 20 manufacturer manufacturer NN cord-349149-nqsohp9h 72 21 's 's POS cord-349149-nqsohp9h 72 22 instructions instruction NNS cord-349149-nqsohp9h 72 23 and and CC cord-349149-nqsohp9h 72 24 sent send VBN cord-349149-nqsohp9h 72 25 for for IN cord-349149-nqsohp9h 72 26 sequencing sequence VBG cord-349149-nqsohp9h 72 27 to to IN cord-349149-nqsohp9h 72 28 x x NN cord-349149-nqsohp9h 72 29 - - : cord-349149-nqsohp9h 72 30 OVO OVO NNP cord-349149-nqsohp9h 72 31 Limited limit VBN cord-349149-nqsohp9h 73 1 ( ( -LRB- cord-349149-nqsohp9h 73 2 x x NN cord-349149-nqsohp9h 73 3 - - HYPH cord-349149-nqsohp9h 73 4 OVO OVO NNP cord-349149-nqsohp9h 73 5 Limited Limited NNP cord-349149-nqsohp9h 73 6 Laboratories Laboratories NNPS cord-349149-nqsohp9h 73 7 , , , cord-349149-nqsohp9h 73 8 Thomson Thomson NNP cord-349149-nqsohp9h 73 9 Cooper Cooper NNP cord-349149-nqsohp9h 73 10 , , , cord-349149-nqsohp9h 73 11 United United NNP cord-349149-nqsohp9h 73 12 Kingdom Kingdom NNP cord-349149-nqsohp9h 73 13 ) ) -RRB- cord-349149-nqsohp9h 73 14 . . . cord-349149-nqsohp9h 74 1 The the DT cord-349149-nqsohp9h 74 2 nucleotide nucleotide JJ cord-349149-nqsohp9h 74 3 and and CC cord-349149-nqsohp9h 74 4 amino amino NN cord-349149-nqsohp9h 74 5 acid acid NN cord-349149-nqsohp9h 74 6 sequences sequence NNS cord-349149-nqsohp9h 74 7 of of IN cord-349149-nqsohp9h 74 8 the the DT cord-349149-nqsohp9h 74 9 S1 S1 NNP cord-349149-nqsohp9h 74 10 gene gene NN cord-349149-nqsohp9h 74 11 of of IN cord-349149-nqsohp9h 74 12 the the DT cord-349149-nqsohp9h 74 13 IBV IBV NNP cord-349149-nqsohp9h 74 14 isolates isolate NNS cord-349149-nqsohp9h 74 15 were be VBD cord-349149-nqsohp9h 74 16 assembled assemble VBN cord-349149-nqsohp9h 74 17 , , , cord-349149-nqsohp9h 74 18 aligned align VBN cord-349149-nqsohp9h 74 19 , , , cord-349149-nqsohp9h 74 20 and and CC cord-349149-nqsohp9h 74 21 compared compare VBN cord-349149-nqsohp9h 74 22 with with IN cord-349149-nqsohp9h 74 23 reference reference NN cord-349149-nqsohp9h 74 24 IBV IBV NNP cord-349149-nqsohp9h 74 25 strains strain NNS cord-349149-nqsohp9h 74 26 using use VBG cord-349149-nqsohp9h 74 27 BASIC basic JJ cord-349149-nqsohp9h 74 28 BLAST blast NN cord-349149-nqsohp9h 74 29 program program NN cord-349149-nqsohp9h 74 30 in in IN cord-349149-nqsohp9h 74 31 DNAStar DNAStar NNP cord-349149-nqsohp9h 74 32 . . . cord-349149-nqsohp9h 75 1 Phylogenetic phylogenetic JJ cord-349149-nqsohp9h 75 2 analysis analysis NN cord-349149-nqsohp9h 75 3 of of IN cord-349149-nqsohp9h 75 4 the the DT cord-349149-nqsohp9h 75 5 S1 s1 NN cord-349149-nqsohp9h 75 6 amino amino NN cord-349149-nqsohp9h 75 7 acid acid NN cord-349149-nqsohp9h 75 8 sequences sequence NNS cord-349149-nqsohp9h 75 9 was be VBD cord-349149-nqsohp9h 75 10 performed perform VBN cord-349149-nqsohp9h 75 11 with with IN cord-349149-nqsohp9h 75 12 the the DT cord-349149-nqsohp9h 75 13 neighbor neighbor NN cord-349149-nqsohp9h 75 14 - - HYPH cord-349149-nqsohp9h 75 15 joining join VBG cord-349149-nqsohp9h 75 16 method method NN cord-349149-nqsohp9h 75 17 using use VBG cord-349149-nqsohp9h 75 18 the the DT cord-349149-nqsohp9h 75 19 DNAStar dnastar NN cord-349149-nqsohp9h 75 20 software software NN cord-349149-nqsohp9h 75 21 [ [ -LRB- cord-349149-nqsohp9h 75 22 18 18 CD cord-349149-nqsohp9h 75 23 ] ] -RRB- cord-349149-nqsohp9h 75 24 . . . cord-349149-nqsohp9h 76 1 The the DT cord-349149-nqsohp9h 76 2 partial partial JJ cord-349149-nqsohp9h 76 3 S1 s1 NN cord-349149-nqsohp9h 76 4 nucleotide nucleotide JJ cord-349149-nqsohp9h 76 5 sequences sequence NNS cord-349149-nqsohp9h 76 6 from from IN cord-349149-nqsohp9h 76 7 the the DT cord-349149-nqsohp9h 76 8 IBV IBV NNP cord-349149-nqsohp9h 76 9 isolates isolate NNS cord-349149-nqsohp9h 76 10 have have VBP cord-349149-nqsohp9h 76 11 been be VBN cord-349149-nqsohp9h 76 12 submitted submit VBN cord-349149-nqsohp9h 76 13 to to IN cord-349149-nqsohp9h 76 14 the the DT cord-349149-nqsohp9h 76 15 GenBank GenBank NNP cord-349149-nqsohp9h 76 16 database database NN cord-349149-nqsohp9h 76 17 and and CC cord-349149-nqsohp9h 76 18 assigned assign VBD cord-349149-nqsohp9h 76 19 the the DT cord-349149-nqsohp9h 76 20 accession accession NN cord-349149-nqsohp9h 76 21 number number NN cord-349149-nqsohp9h 76 22 MG252734 MG252734 NNP cord-349149-nqsohp9h 76 23 . . . cord-349149-nqsohp9h 77 1 The the DT cord-349149-nqsohp9h 77 2 nucleotide nucleotide JJ cord-349149-nqsohp9h 77 3 and and CC cord-349149-nqsohp9h 77 4 amino amino NN cord-349149-nqsohp9h 77 5 acid acid NN cord-349149-nqsohp9h 77 6 sequences sequence NNS cord-349149-nqsohp9h 77 7 of of IN cord-349149-nqsohp9h 77 8 the the DT cord-349149-nqsohp9h 77 9 S1 S1 NNP cord-349149-nqsohp9h 77 10 gene gene NN cord-349149-nqsohp9h 77 11 of of IN cord-349149-nqsohp9h 77 12 IBV IBV NNP cord-349149-nqsohp9h 77 13 strains strain NNS cord-349149-nqsohp9h 77 14 used use VBN cord-349149-nqsohp9h 77 15 for for IN cord-349149-nqsohp9h 77 16 the the DT cord-349149-nqsohp9h 77 17 comparison comparison NN cord-349149-nqsohp9h 77 18 are be VBP cord-349149-nqsohp9h 77 19 available available JJ cord-349149-nqsohp9h 77 20 in in IN cord-349149-nqsohp9h 77 21 GenBank GenBank NNP cord-349149-nqsohp9h 77 22 under under IN cord-349149-nqsohp9h 77 23 the the DT cord-349149-nqsohp9h 77 24 following follow VBG cord-349149-nqsohp9h 77 25 accession accession NN cord-349149-nqsohp9h 77 26 numbers number NNS cord-349149-nqsohp9h 77 27 : : : cord-349149-nqsohp9h 77 28 CR CR NNP cord-349149-nqsohp9h 77 29 88 88 CD cord-349149-nqsohp9h 77 30 ( ( -LRB- cord-349149-nqsohp9h 77 31 KM067900 KM067900 NNP cord-349149-nqsohp9h 77 32 ) ) -RRB- cord-349149-nqsohp9h 77 33 , , , cord-349149-nqsohp9h 77 34 4/91 4/91 CD cord-349149-nqsohp9h 77 35 ( ( -LRB- cord-349149-nqsohp9h 77 36 JN600614 jn600614 NN cord-349149-nqsohp9h 77 37 ) ) -RRB- cord-349149-nqsohp9h 77 38 , , , cord-349149-nqsohp9h 77 39 Qx Qx NNP cord-349149-nqsohp9h 77 40 ( ( -LRB- cord-349149-nqsohp9h 77 41 AF193423 AF193423 NNP cord-349149-nqsohp9h 77 42 ) ) -RRB- cord-349149-nqsohp9h 77 43 , , , cord-349149-nqsohp9h 77 44 Ma5 Ma5 NNS cord-349149-nqsohp9h 77 45 ( ( -LRB- cord-349149-nqsohp9h 77 46 AY561713 ay561713 NN cord-349149-nqsohp9h 77 47 ) ) -RRB- cord-349149-nqsohp9h 77 48 , , , cord-349149-nqsohp9h 77 49 H120 H120 NNP cord-349149-nqsohp9h 77 50 ( ( -LRB- cord-349149-nqsohp9h 77 51 M21970 M21970 NNP cord-349149-nqsohp9h 77 52 ) ) -RRB- cord-349149-nqsohp9h 77 53 , , , cord-349149-nqsohp9h 77 54 M41 M41 NNP cord-349149-nqsohp9h 77 55 ( ( -LRB- cord-349149-nqsohp9h 77 56 GQ219712 gq219712 NN cord-349149-nqsohp9h 77 57 ) ) -RRB- cord-349149-nqsohp9h 77 58 , , , cord-349149-nqsohp9h 77 59 D274 D274 NNP cord-349149-nqsohp9h 77 60 ( ( -LRB- cord-349149-nqsohp9h 77 61 KR106999 kr106999 NN cord-349149-nqsohp9h 77 62 ) ) -RRB- cord-349149-nqsohp9h 77 63 , , , cord-349149-nqsohp9h 77 64 Italy Italy NNP cord-349149-nqsohp9h 77 65 02 02 CD cord-349149-nqsohp9h 77 66 ( ( -LRB- cord-349149-nqsohp9h 77 67 AJ457137 AJ457137 NNP cord-349149-nqsohp9h 77 68 ) ) -RRB- cord-349149-nqsohp9h 77 69 , , , cord-349149-nqsohp9h 77 70 and and CC cord-349149-nqsohp9h 77 71 QX QX NNP cord-349149-nqsohp9h 77 72 D388 D388 NNS cord-349149-nqsohp9h 77 73 ( ( -LRB- cord-349149-nqsohp9h 77 74 DQ674739 DQ674739 NNP cord-349149-nqsohp9h 77 75 ) ) -RRB- cord-349149-nqsohp9h 77 76 . . . cord-349149-nqsohp9h 78 1 The the DT cord-349149-nqsohp9h 78 2 specific specific JJ cord-349149-nqsohp9h 78 3 IBV IBV NNP cord-349149-nqsohp9h 78 4 strains strain NNS cord-349149-nqsohp9h 78 5 that that WDT cord-349149-nqsohp9h 78 6 were be VBD cord-349149-nqsohp9h 78 7 used use VBN cord-349149-nqsohp9h 78 8 in in IN cord-349149-nqsohp9h 78 9 this this DT cord-349149-nqsohp9h 78 10 study study NN cord-349149-nqsohp9h 78 11 were be VBD cord-349149-nqsohp9h 79 1 M-41 M-41 NNP cord-349149-nqsohp9h 79 2 ( ( -LRB- cord-349149-nqsohp9h 79 3 a a DT cord-349149-nqsohp9h 79 4 US US NNP cord-349149-nqsohp9h 79 5 strain strain NN cord-349149-nqsohp9h 79 6 ) ) -RRB- cord-349149-nqsohp9h 79 7 , , , cord-349149-nqsohp9h 79 8 4/91 4/91 CD cord-349149-nqsohp9h 79 9 ( ( -LRB- cord-349149-nqsohp9h 79 10 European european JJ cord-349149-nqsohp9h 79 11 vaccine vaccine NN cord-349149-nqsohp9h 79 12 strain strain NN cord-349149-nqsohp9h 79 13 ) ) -RRB- cord-349149-nqsohp9h 79 14 , , , cord-349149-nqsohp9h 79 15 D274 D274 '' cord-349149-nqsohp9h 79 16 ( ( -LRB- cord-349149-nqsohp9h 79 17 a a DT cord-349149-nqsohp9h 79 18 Dutch dutch JJ cord-349149-nqsohp9h 79 19 field field NN cord-349149-nqsohp9h 79 20 isolate isolate VB cord-349149-nqsohp9h 79 21 ) ) -RRB- cord-349149-nqsohp9h 79 22 , , , cord-349149-nqsohp9h 79 23 and and CC cord-349149-nqsohp9h 79 24 QX QX NNP cord-349149-nqsohp9h 79 25 ( ( -LRB- cord-349149-nqsohp9h 79 26 Chinese chinese JJ cord-349149-nqsohp9h 79 27 strain strain NN cord-349149-nqsohp9h 79 28 ) ) -RRB- cord-349149-nqsohp9h 79 29 . . . cord-349149-nqsohp9h 80 1 HI HI NNP cord-349149-nqsohp9h 80 2 test test NN cord-349149-nqsohp9h 80 3 was be VBD cord-349149-nqsohp9h 80 4 performed perform VBN cord-349149-nqsohp9h 80 5 using use VBG cord-349149-nqsohp9h 80 6 the the DT cord-349149-nqsohp9h 80 7 Available available JJ cord-349149-nqsohp9h 80 8 at at IN cord-349149-nqsohp9h 80 9 www.veterinaryworld.org/Vol.11/November-2018/17.pdf www.veterinaryworld.org/Vol.11/November-2018/17.pdf VBG cord-349149-nqsohp9h 80 10 FLOKSCREEN FLOKSCREEN NNP cord-349149-nqsohp9h 80 11 IB IB NNP cord-349149-nqsohp9h 80 12 - - HYPH cord-349149-nqsohp9h 80 13 HI HI NNP cord-349149-nqsohp9h 80 14 TEST test NN cord-349149-nqsohp9h 80 15 kit kit NN cord-349149-nqsohp9h 80 16 according accord VBG cord-349149-nqsohp9h 80 17 to to IN cord-349149-nqsohp9h 80 18 the the DT cord-349149-nqsohp9h 80 19 manufacturer manufacturer NN cord-349149-nqsohp9h 80 20 's 's POS cord-349149-nqsohp9h 80 21 instructions instruction NNS cord-349149-nqsohp9h 80 22 . . . cord-349149-nqsohp9h 81 1 Briefly briefly RB cord-349149-nqsohp9h 81 2 , , , cord-349149-nqsohp9h 81 3 the the DT cord-349149-nqsohp9h 81 4 sera sera NNP cord-349149-nqsohp9h 81 5 were be VBD cord-349149-nqsohp9h 81 6 serially serially RB cord-349149-nqsohp9h 81 7 diluted dilute VBN cord-349149-nqsohp9h 81 8 and and CC cord-349149-nqsohp9h 81 9 mixed mix VBN cord-349149-nqsohp9h 81 10 with with IN cord-349149-nqsohp9h 81 11 an an DT cord-349149-nqsohp9h 81 12 equivalent equivalent JJ cord-349149-nqsohp9h 81 13 volume volume NN cord-349149-nqsohp9h 81 14 of of IN cord-349149-nqsohp9h 81 15 the the DT cord-349149-nqsohp9h 81 16 antigen antigen NN cord-349149-nqsohp9h 81 17 . . . cord-349149-nqsohp9h 82 1 After after IN cord-349149-nqsohp9h 82 2 30 30 CD cord-349149-nqsohp9h 82 3 min min NN cord-349149-nqsohp9h 82 4 of of IN cord-349149-nqsohp9h 82 5 incubation incubation NN cord-349149-nqsohp9h 82 6 at at IN cord-349149-nqsohp9h 82 7 20 20 CD cord-349149-nqsohp9h 82 8 ° ° NN cord-349149-nqsohp9h 82 9 C c NN cord-349149-nqsohp9h 82 10 , , , cord-349149-nqsohp9h 82 11 the the DT cord-349149-nqsohp9h 82 12 red red JJ cord-349149-nqsohp9h 82 13 blood blood NN cord-349149-nqsohp9h 82 14 cells cell NNS cord-349149-nqsohp9h 82 15 of of IN cord-349149-nqsohp9h 82 16 SPF SPF NNP cord-349149-nqsohp9h 82 17 chickens chicken NNS cord-349149-nqsohp9h 82 18 ( ( -LRB- cord-349149-nqsohp9h 82 19 anticoagulant anticoagulant JJ cord-349149-nqsohp9h 82 20 solution solution NN cord-349149-nqsohp9h 82 21 ) ) -RRB- cord-349149-nqsohp9h 82 22 were be VBD cord-349149-nqsohp9h 82 23 added add VBN cord-349149-nqsohp9h 82 24 and and CC cord-349149-nqsohp9h 82 25 incubated incubate VBN cord-349149-nqsohp9h 82 26 further further RB cord-349149-nqsohp9h 82 27 for for IN cord-349149-nqsohp9h 82 28 40 40 CD cord-349149-nqsohp9h 82 29 min min NN cord-349149-nqsohp9h 82 30 at at IN cord-349149-nqsohp9h 82 31 20 20 CD cord-349149-nqsohp9h 82 32 ° ° NN cord-349149-nqsohp9h 82 33 C c NN cord-349149-nqsohp9h 82 34 . . . cord-349149-nqsohp9h 83 1 The the DT cord-349149-nqsohp9h 83 2 plates plate NNS cord-349149-nqsohp9h 83 3 were be VBD cord-349149-nqsohp9h 83 4 then then RB cord-349149-nqsohp9h 83 5 tilted tilt VBN cord-349149-nqsohp9h 83 6 to to TO cord-349149-nqsohp9h 83 7 observe observe VB cord-349149-nqsohp9h 83 8 inhibition inhibition NN cord-349149-nqsohp9h 83 9 of of IN cord-349149-nqsohp9h 83 10 hemagglutination hemagglutination NN cord-349149-nqsohp9h 83 11 . . . cord-349149-nqsohp9h 84 1 Statistical Statistical NNP cord-349149-nqsohp9h 84 2 Package Package NNP cord-349149-nqsohp9h 84 3 for for IN cord-349149-nqsohp9h 84 4 the the DT cord-349149-nqsohp9h 84 5 Social Social NNP cord-349149-nqsohp9h 84 6 Sciences Sciences NNPS cord-349149-nqsohp9h 84 7 version version NN cord-349149-nqsohp9h 84 8 20 20 CD cord-349149-nqsohp9h 84 9 ( ( -LRB- cord-349149-nqsohp9h 84 10 IBM IBM NNP cord-349149-nqsohp9h 84 11 , , , cord-349149-nqsohp9h 84 12 USA USA NNP cord-349149-nqsohp9h 84 13 ) ) -RRB- cord-349149-nqsohp9h 84 14 was be VBD cord-349149-nqsohp9h 84 15 used use VBN cord-349149-nqsohp9h 84 16 for for IN cord-349149-nqsohp9h 84 17 statistical statistical JJ cord-349149-nqsohp9h 84 18 analysis analysis NN cord-349149-nqsohp9h 84 19 . . . cord-349149-nqsohp9h 85 1 The the DT cord-349149-nqsohp9h 85 2 geometric geometric JJ cord-349149-nqsohp9h 85 3 mean mean NN cord-349149-nqsohp9h 85 4 titer titer NN cord-349149-nqsohp9h 85 5 ( ( -LRB- cord-349149-nqsohp9h 85 6 GMT GMT NNP cord-349149-nqsohp9h 85 7 ) ) -RRB- cord-349149-nqsohp9h 85 8 was be VBD cord-349149-nqsohp9h 85 9 calculated calculate VBN cord-349149-nqsohp9h 85 10 by by IN cord-349149-nqsohp9h 85 11 counting count VBG cord-349149-nqsohp9h 85 12 the the DT cord-349149-nqsohp9h 85 13 number number NN cord-349149-nqsohp9h 85 14 of of IN cord-349149-nqsohp9h 85 15 wells well NNS cord-349149-nqsohp9h 85 16 from from IN cord-349149-nqsohp9h 85 17 all all DT cord-349149-nqsohp9h 85 18 serum serum NN cord-349149-nqsohp9h 85 19 samples sample NNS cord-349149-nqsohp9h 85 20 within within IN cord-349149-nqsohp9h 85 21 one one CD cord-349149-nqsohp9h 85 22 flock flock NN cord-349149-nqsohp9h 85 23 showing show VBG cord-349149-nqsohp9h 85 24 HI HI NNP cord-349149-nqsohp9h 85 25 activity activity NN cord-349149-nqsohp9h 85 26 , , , cord-349149-nqsohp9h 85 27 and and CC cord-349149-nqsohp9h 85 28 the the DT cord-349149-nqsohp9h 85 29 average average JJ cord-349149-nqsohp9h 85 30 number number NN cord-349149-nqsohp9h 85 31 was be VBD cord-349149-nqsohp9h 85 32 then then RB cord-349149-nqsohp9h 85 33 cross cross JJ cord-349149-nqsohp9h 85 34 - - VBN cord-349149-nqsohp9h 85 35 checked check VBN cord-349149-nqsohp9h 85 36 against against IN cord-349149-nqsohp9h 85 37 GMT GMT NNP cord-349149-nqsohp9h 85 38 values value NNS cord-349149-nqsohp9h 85 39 given give VBN cord-349149-nqsohp9h 85 40 in in IN cord-349149-nqsohp9h 85 41 Brug Brug NNP cord-349149-nqsohp9h 85 42 's 's POS cord-349149-nqsohp9h 85 43 table table NN cord-349149-nqsohp9h 85 44 . . . cord-349149-nqsohp9h 86 1 The the DT cord-349149-nqsohp9h 86 2 results result NNS cord-349149-nqsohp9h 86 3 were be VBD cord-349149-nqsohp9h 86 4 reported report VBN cord-349149-nqsohp9h 86 5 as as IN cord-349149-nqsohp9h 86 6 the the DT cord-349149-nqsohp9h 86 7 GMT GMT NNP cord-349149-nqsohp9h 86 8 for for IN cord-349149-nqsohp9h 86 9 a a DT cord-349149-nqsohp9h 86 10 given give VBN cord-349149-nqsohp9h 86 11 flock flock NN cord-349149-nqsohp9h 86 12 against against IN cord-349149-nqsohp9h 86 13 a a DT cord-349149-nqsohp9h 86 14 particular particular JJ cord-349149-nqsohp9h 86 15 IBV IBV NNP cord-349149-nqsohp9h 86 16 antigen antigen NN cord-349149-nqsohp9h 86 17 . . . cord-349149-nqsohp9h 87 1 Descriptive descriptive JJ cord-349149-nqsohp9h 87 2 statistics statistic NNS cord-349149-nqsohp9h 87 3 including include VBG cord-349149-nqsohp9h 87 4 percentages percentage NNS cord-349149-nqsohp9h 87 5 , , , cord-349149-nqsohp9h 87 6 means mean NNS cord-349149-nqsohp9h 87 7 , , , cord-349149-nqsohp9h 87 8 and and CC cord-349149-nqsohp9h 87 9 frequency frequency NN cord-349149-nqsohp9h 87 10 distribution distribution NN cord-349149-nqsohp9h 87 11 were be VBD cord-349149-nqsohp9h 87 12 calculated calculate VBN cord-349149-nqsohp9h 87 13 for for IN cord-349149-nqsohp9h 87 14 each each DT cord-349149-nqsohp9h 87 15 of of IN cord-349149-nqsohp9h 87 16 the the DT cord-349149-nqsohp9h 87 17 variables variable NNS cord-349149-nqsohp9h 87 18 . . . cord-349149-nqsohp9h 88 1 Information information NN cord-349149-nqsohp9h 88 2 regarding regard VBG cord-349149-nqsohp9h 88 3 the the DT cord-349149-nqsohp9h 88 4 sampling sampling NN cord-349149-nqsohp9h 88 5 date date NN cord-349149-nqsohp9h 88 6 , , , cord-349149-nqsohp9h 88 7 biosecurity biosecurity NN cord-349149-nqsohp9h 88 8 status status NN cord-349149-nqsohp9h 88 9 , , , cord-349149-nqsohp9h 88 10 region region NN cord-349149-nqsohp9h 88 11 of of IN cord-349149-nqsohp9h 88 12 sampling sampling NN cord-349149-nqsohp9h 88 13 , , , cord-349149-nqsohp9h 88 14 vaccination vaccination NN cord-349149-nqsohp9h 88 15 programs program NNS cord-349149-nqsohp9h 88 16 , , , cord-349149-nqsohp9h 88 17 necropsic necropsic JJ cord-349149-nqsohp9h 88 18 and and CC cord-349149-nqsohp9h 88 19 clinical clinical JJ cord-349149-nqsohp9h 88 20 signs sign NNS cord-349149-nqsohp9h 88 21 , , , cord-349149-nqsohp9h 88 22 the the DT cord-349149-nqsohp9h 88 23 age age NN cord-349149-nqsohp9h 88 24 of of IN cord-349149-nqsohp9h 88 25 the the DT cord-349149-nqsohp9h 88 26 broilers broiler NNS cord-349149-nqsohp9h 88 27 , , , cord-349149-nqsohp9h 88 28 and and CC cord-349149-nqsohp9h 88 29 mortality mortality NN cord-349149-nqsohp9h 88 30 rates rate NNS cord-349149-nqsohp9h 88 31 are be VBP cord-349149-nqsohp9h 88 32 summarized summarize VBN cord-349149-nqsohp9h 88 33 in in IN cord-349149-nqsohp9h 88 34 Table- Table- NNP cord-349149-nqsohp9h 88 35 1 1 CD cord-349149-nqsohp9h 88 36 . . . cord-349149-nqsohp9h 89 1 We -PRON- PRP cord-349149-nqsohp9h 89 2 collected collect VBD cord-349149-nqsohp9h 89 3 14 14 CD cord-349149-nqsohp9h 89 4 samples sample NNS cord-349149-nqsohp9h 89 5 from from IN cord-349149-nqsohp9h 89 6 broiler broiler NN cord-349149-nqsohp9h 89 7 farms farm NNS cord-349149-nqsohp9h 89 8 located locate VBN cord-349149-nqsohp9h 89 9 in in IN cord-349149-nqsohp9h 89 10 Western western JJ cord-349149-nqsohp9h 89 11 and and CC cord-349149-nqsohp9h 89 12 Central Central NNP cord-349149-nqsohp9h 89 13 Algeria Algeria NNP cord-349149-nqsohp9h 89 14 from from IN cord-349149-nqsohp9h 89 15 the the DT cord-349149-nqsohp9h 89 16 flocks flock NNS cord-349149-nqsohp9h 89 17 showing show VBG cord-349149-nqsohp9h 89 18 kidney kidney NN cord-349149-nqsohp9h 89 19 damage damage NN cord-349149-nqsohp9h 89 20 and and CC cord-349149-nqsohp9h 89 21 high high JJ cord-349149-nqsohp9h 89 22 mortality mortality NN cord-349149-nqsohp9h 89 23 rates rate NNS cord-349149-nqsohp9h 89 24 . . . cord-349149-nqsohp9h 90 1 Eight eight CD cord-349149-nqsohp9h 90 2 of of IN cord-349149-nqsohp9h 90 3 these these DT cord-349149-nqsohp9h 90 4 farms farm NNS cord-349149-nqsohp9h 90 5 had have VBD cord-349149-nqsohp9h 90 6 not not RB cord-349149-nqsohp9h 90 7 vaccinated vaccinate VBN cord-349149-nqsohp9h 90 8 their -PRON- PRP$ cord-349149-nqsohp9h 90 9 flocks flock NNS cord-349149-nqsohp9h 90 10 against against IN cord-349149-nqsohp9h 90 11 IBV IBV NNP cord-349149-nqsohp9h 90 12 , , , cord-349149-nqsohp9h 90 13 2 2 CD cord-349149-nqsohp9h 90 14 farms farm NNS cord-349149-nqsohp9h 90 15 conducted conduct VBD cord-349149-nqsohp9h 90 16 the the DT cord-349149-nqsohp9h 90 17 prototype prototype NN cord-349149-nqsohp9h 90 18 vaccination vaccination NN cord-349149-nqsohp9h 90 19 program program NN cord-349149-nqsohp9h 90 20 but but CC cord-349149-nqsohp9h 90 21 with with IN cord-349149-nqsohp9h 90 22 a a DT cord-349149-nqsohp9h 90 23 short short JJ cord-349149-nqsohp9h 90 24 interval interval NN cord-349149-nqsohp9h 90 25 between between IN cord-349149-nqsohp9h 90 26 the the DT cord-349149-nqsohp9h 90 27 first first JJ cord-349149-nqsohp9h 90 28 and and CC cord-349149-nqsohp9h 90 29 second second JJ cord-349149-nqsohp9h 90 30 vaccinations vaccination NNS cord-349149-nqsohp9h 90 31 , , , cord-349149-nqsohp9h 90 32 and and CC cord-349149-nqsohp9h 90 33 4 4 CD cord-349149-nqsohp9h 90 34 farms farm NNS cord-349149-nqsohp9h 90 35 vaccinated vaccinate VBD cord-349149-nqsohp9h 90 36 their -PRON- PRP$ cord-349149-nqsohp9h 90 37 broilers broiler NNS cord-349149-nqsohp9h 90 38 only only RB cord-349149-nqsohp9h 90 39 against against IN cord-349149-nqsohp9h 90 40 the the DT cord-349149-nqsohp9h 90 41 Mass Mass NNP cord-349149-nqsohp9h 90 42 serotype serotype NN cord-349149-nqsohp9h 90 43 . . . cord-349149-nqsohp9h 91 1 All all DT cord-349149-nqsohp9h 91 2 vaccinations vaccination NNS cord-349149-nqsohp9h 91 3 were be VBD cord-349149-nqsohp9h 91 4 performed perform VBN cord-349149-nqsohp9h 91 5 through through IN cord-349149-nqsohp9h 91 6 the the DT cord-349149-nqsohp9h 91 7 drinking drinking NN cord-349149-nqsohp9h 91 8 water water NN cord-349149-nqsohp9h 91 9 route route NN cord-349149-nqsohp9h 91 10 , , , cord-349149-nqsohp9h 91 11 and and CC cord-349149-nqsohp9h 91 12 all all PDT cord-349149-nqsohp9h 91 13 the the DT cord-349149-nqsohp9h 91 14 sampled sample VBN cord-349149-nqsohp9h 91 15 broiler broiler NN cord-349149-nqsohp9h 91 16 farms farm NNS cord-349149-nqsohp9h 91 17 showed show VBD cord-349149-nqsohp9h 91 18 poor poor JJ cord-349149-nqsohp9h 91 19 zootechnical zootechnical JJ cord-349149-nqsohp9h 91 20 performance performance NN cord-349149-nqsohp9h 91 21 with with IN cord-349149-nqsohp9h 91 22 rare rare JJ cord-349149-nqsohp9h 91 23 period period NN cord-349149-nqsohp9h 91 24 elongation elongation NN cord-349149-nqsohp9h 91 25 ( ( -LRB- cord-349149-nqsohp9h 91 26 until until IN cord-349149-nqsohp9h 91 27 71 71 CD cord-349149-nqsohp9h 91 28 days day NNS cord-349149-nqsohp9h 91 29 in in IN cord-349149-nqsohp9h 91 30 farm farm NN cord-349149-nqsohp9h 91 31 2 2 CD cord-349149-nqsohp9h 91 32 ) ) -RRB- cord-349149-nqsohp9h 91 33 and and CC cord-349149-nqsohp9h 91 34 high high JJ cord-349149-nqsohp9h 91 35 mortality mortality NN cord-349149-nqsohp9h 91 36 rates rate NNS cord-349149-nqsohp9h 91 37 ( ( -LRB- cord-349149-nqsohp9h 91 38 up up IN cord-349149-nqsohp9h 91 39 to to TO cord-349149-nqsohp9h 91 40 50 50 CD cord-349149-nqsohp9h 91 41 % % NN cord-349149-nqsohp9h 91 42 in in IN cord-349149-nqsohp9h 91 43 farms farm NNS cord-349149-nqsohp9h 91 44 11 11 CD cord-349149-nqsohp9h 91 45 and and CC cord-349149-nqsohp9h 91 46 13 13 CD cord-349149-nqsohp9h 91 47 ) ) -RRB- cord-349149-nqsohp9h 91 48 . . . cord-349149-nqsohp9h 92 1 The the DT cord-349149-nqsohp9h 92 2 seroprevalence seroprevalence NN cord-349149-nqsohp9h 92 3 of of IN cord-349149-nqsohp9h 92 4 various various JJ cord-349149-nqsohp9h 92 5 IBV IBV NNP cord-349149-nqsohp9h 92 6 strains strain NNS cord-349149-nqsohp9h 92 7 in in IN cord-349149-nqsohp9h 92 8 broiler broiler NN cord-349149-nqsohp9h 92 9 flocks flock NNS cord-349149-nqsohp9h 92 10 suffering suffer VBG cord-349149-nqsohp9h 92 11 from from IN cord-349149-nqsohp9h 92 12 kidney kidney NN cord-349149-nqsohp9h 92 13 damage damage NN cord-349149-nqsohp9h 92 14 in in IN cord-349149-nqsohp9h 92 15 Algeria Algeria NNP cord-349149-nqsohp9h 92 16 is be VBZ cord-349149-nqsohp9h 92 17 summarized summarize VBN cord-349149-nqsohp9h 92 18 in in IN cord-349149-nqsohp9h 92 19 Table- Table- NNP cord-349149-nqsohp9h 92 20 2 2 CD cord-349149-nqsohp9h 92 21 . . . cord-349149-nqsohp9h 93 1 All all PDT cord-349149-nqsohp9h 93 2 the the DT cord-349149-nqsohp9h 93 3 broiler broiler NN cord-349149-nqsohp9h 93 4 flocks flock NNS cord-349149-nqsohp9h 93 5 ( ( -LRB- cord-349149-nqsohp9h 93 6 100 100 CD cord-349149-nqsohp9h 93 7 % % NN cord-349149-nqsohp9h 93 8 ) ) -RRB- cord-349149-nqsohp9h 93 9 tested test VBN cord-349149-nqsohp9h 93 10 in in IN cord-349149-nqsohp9h 93 11 this this DT cord-349149-nqsohp9h 93 12 study study NN cord-349149-nqsohp9h 93 13 were be VBD cord-349149-nqsohp9h 93 14 seropositive seropositive JJ cord-349149-nqsohp9h 93 15 for for IN cord-349149-nqsohp9h 93 16 the the DT cord-349149-nqsohp9h 93 17 QX QX NNP cord-349149-nqsohp9h 93 18 variant variant NN cord-349149-nqsohp9h 93 19 strain strain NN cord-349149-nqsohp9h 93 20 , , , cord-349149-nqsohp9h 93 21 with with IN cord-349149-nqsohp9h 93 22 high high JJ cord-349149-nqsohp9h 93 23 mean mean NN cord-349149-nqsohp9h 93 24 titer titer NN cord-349149-nqsohp9h 93 25 levels level NNS cord-349149-nqsohp9h 93 26 ( ( -LRB- cord-349149-nqsohp9h 93 27 MT=11 MT=11 NNP cord-349149-nqsohp9h 93 28 log log NN cord-349149-nqsohp9h 93 29 2 2 CD cord-349149-nqsohp9h 93 30 ) ) -RRB- cord-349149-nqsohp9h 94 1 regardless regardless RB cord-349149-nqsohp9h 94 2 of of IN cord-349149-nqsohp9h 94 3 the the DT cord-349149-nqsohp9h 94 4 vaccination vaccination NN cord-349149-nqsohp9h 94 5 status status NN cord-349149-nqsohp9h 94 6 . . . cord-349149-nqsohp9h 95 1 Furthermore furthermore RB cord-349149-nqsohp9h 95 2 , , , cord-349149-nqsohp9h 95 3 60 60 CD cord-349149-nqsohp9h 95 4 % % NN cord-349149-nqsohp9h 95 5 and and CC cord-349149-nqsohp9h 95 6 50 50 CD cord-349149-nqsohp9h 95 7 % % NN cord-349149-nqsohp9h 95 8 of of IN cord-349149-nqsohp9h 95 9 the the DT cord-349149-nqsohp9h 95 10 broiler broiler NN cord-349149-nqsohp9h 95 11 flocks flock NNS cord-349149-nqsohp9h 95 12 were be VBD cord-349149-nqsohp9h 95 13 seropositive seropositive JJ cord-349149-nqsohp9h 95 14 for for IN cord-349149-nqsohp9h 95 15 antibodies antibody NNS cord-349149-nqsohp9h 95 16 against against IN cord-349149-nqsohp9h 95 17 the the DT cord-349149-nqsohp9h 95 18 4/91 4/91 CD cord-349149-nqsohp9h 95 19 and and CC cord-349149-nqsohp9h 95 20 M-41 M-41 NNP cord-349149-nqsohp9h 95 21 strains strain NNS cord-349149-nqsohp9h 95 22 , , , cord-349149-nqsohp9h 95 23 respectively respectively RB cord-349149-nqsohp9h 95 24 , , , cord-349149-nqsohp9h 95 25 although although IN cord-349149-nqsohp9h 95 26 with with IN cord-349149-nqsohp9h 95 27 low low JJ cord-349149-nqsohp9h 95 28 mean mean NN cord-349149-nqsohp9h 95 29 titer titer NN cord-349149-nqsohp9h 95 30 levels level NNS cord-349149-nqsohp9h 95 31 . . . cord-349149-nqsohp9h 96 1 The the DT cord-349149-nqsohp9h 96 2 seroprevalence seroprevalence NN cord-349149-nqsohp9h 96 3 of of IN cord-349149-nqsohp9h 96 4 IBV IBV NNP cord-349149-nqsohp9h 96 5 D-274 d-274 NN cord-349149-nqsohp9h 96 6 was be VBD cord-349149-nqsohp9h 96 7 the the DT cord-349149-nqsohp9h 96 8 lowest low JJS cord-349149-nqsohp9h 96 9 with with IN cord-349149-nqsohp9h 96 10 only only RB cord-349149-nqsohp9h 96 11 7 7 CD cord-349149-nqsohp9h 96 12 % % NN cord-349149-nqsohp9h 96 13 of of IN cord-349149-nqsohp9h 96 14 the the DT cord-349149-nqsohp9h 96 15 broiler broiler NN cord-349149-nqsohp9h 96 16 flocks flock NNS cord-349149-nqsohp9h 96 17 testing test VBG cord-349149-nqsohp9h 96 18 positive positive JJ cord-349149-nqsohp9h 96 19 . . . cord-349149-nqsohp9h 97 1 Six six CD cord-349149-nqsohp9h 97 2 of of IN cord-349149-nqsohp9h 97 3 the the DT cord-349149-nqsohp9h 97 4 14 14 CD cord-349149-nqsohp9h 97 5 broiler broiler NN cord-349149-nqsohp9h 97 6 samples sample NNS cord-349149-nqsohp9h 97 7 were be VBD cord-349149-nqsohp9h 97 8 positive positive JJ cord-349149-nqsohp9h 97 9 for for IN cord-349149-nqsohp9h 97 10 the the DT cord-349149-nqsohp9h 97 11 IBV IBV NNP cord-349149-nqsohp9h 97 12 S1 S1 NNP cord-349149-nqsohp9h 97 13 gene gene NN cord-349149-nqsohp9h 97 14 , , , cord-349149-nqsohp9h 97 15 but but CC cord-349149-nqsohp9h 97 16 only only RB cord-349149-nqsohp9h 97 17 two two CD cord-349149-nqsohp9h 97 18 isolates isolate NNS cord-349149-nqsohp9h 97 19 were be VBD cord-349149-nqsohp9h 97 20 typable typable JJ cord-349149-nqsohp9h 97 21 by by IN cord-349149-nqsohp9h 97 22 sequencing sequence VBG cord-349149-nqsohp9h 97 23 and and CC cord-349149-nqsohp9h 97 24 showed show VBD cord-349149-nqsohp9h 97 25 a a DT cord-349149-nqsohp9h 97 26 high high JJ cord-349149-nqsohp9h 97 27 load load NN cord-349149-nqsohp9h 97 28 of of IN cord-349149-nqsohp9h 97 29 IB IB NNP cord-349149-nqsohp9h 97 30 viral viral JJ cord-349149-nqsohp9h 97 31 RNA RNA NNP cord-349149-nqsohp9h 97 32 ( ( -LRB- cord-349149-nqsohp9h 97 33 Ct Ct NNP cord-349149-nqsohp9h 97 34 1 1 CD cord-349149-nqsohp9h 97 35 = = SYM cord-349149-nqsohp9h 97 36 22.15 22.15 CD cord-349149-nqsohp9h 97 37 and and CC cord-349149-nqsohp9h 97 38 Ct Ct NNP cord-349149-nqsohp9h 97 39 2 2 CD cord-349149-nqsohp9h 97 40 = = SYM cord-349149-nqsohp9h 97 41 31.4 31.4 CD cord-349149-nqsohp9h 97 42 ) ) -RRB- cord-349149-nqsohp9h 97 43 . . . cord-349149-nqsohp9h 98 1 One one CD cord-349149-nqsohp9h 98 2 of of IN cord-349149-nqsohp9h 98 3 the the DT cord-349149-nqsohp9h 98 4 two two CD cord-349149-nqsohp9h 98 5 sequences sequence NNS cord-349149-nqsohp9h 98 6 was be VBD cord-349149-nqsohp9h 98 7 identified identify VBN cord-349149-nqsohp9h 98 8 as as IN cord-349149-nqsohp9h 98 9 the the DT cord-349149-nqsohp9h 98 10 IBDZ13a IBDZ13a NNP cord-349149-nqsohp9h 98 11 genotype genotype NN cord-349149-nqsohp9h 98 12 . . . cord-349149-nqsohp9h 99 1 Furthermore furthermore RB cord-349149-nqsohp9h 99 2 , , , cord-349149-nqsohp9h 99 3 IBV IBV NNP cord-349149-nqsohp9h 99 4 was be VBD cord-349149-nqsohp9h 99 5 only only RB cord-349149-nqsohp9h 99 6 detected detect VBN cord-349149-nqsohp9h 99 7 in in IN cord-349149-nqsohp9h 99 8 the the DT cord-349149-nqsohp9h 99 9 kidney kidney NN cord-349149-nqsohp9h 99 10 tissues tissue NNS cord-349149-nqsohp9h 99 11 and and CC cord-349149-nqsohp9h 99 12 not not RB cord-349149-nqsohp9h 99 13 in in IN cord-349149-nqsohp9h 99 14 the the DT cord-349149-nqsohp9h 99 15 trachea trachea NN cord-349149-nqsohp9h 99 16 . . . cord-349149-nqsohp9h 100 1 All all DT cord-349149-nqsohp9h 100 2 samples sample NNS cord-349149-nqsohp9h 100 3 showed show VBD cord-349149-nqsohp9h 100 4 93 93 CD cord-349149-nqsohp9h 100 5 % % NN cord-349149-nqsohp9h 100 6 homology homology NN cord-349149-nqsohp9h 100 7 to to IN cord-349149-nqsohp9h 100 8 the the DT cord-349149-nqsohp9h 100 9 partial partial JJ cord-349149-nqsohp9h 100 10 - - HYPH cord-349149-nqsohp9h 100 11 S1 s1 NN cord-349149-nqsohp9h 100 12 sequence sequence NN cord-349149-nqsohp9h 100 13 acquired acquire VBN cord-349149-nqsohp9h 100 14 from from IN cord-349149-nqsohp9h 100 15 ( ( -LRB- cord-349149-nqsohp9h 100 16 Figure-1 Figure-1 NNP cord-349149-nqsohp9h 100 17 ) ) -RRB- cord-349149-nqsohp9h 100 18 . . . cord-349149-nqsohp9h 101 1 The the DT cord-349149-nqsohp9h 101 2 IBDZ13a ibdz13a NN cord-349149-nqsohp9h 101 3 strain strain NN cord-349149-nqsohp9h 101 4 was be VBD cord-349149-nqsohp9h 101 5 characterized characterize VBN cord-349149-nqsohp9h 101 6 as as IN cord-349149-nqsohp9h 101 7 an an DT cord-349149-nqsohp9h 101 8 IB IB NNP cord-349149-nqsohp9h 101 9 4/91 4/91 CD cord-349149-nqsohp9h 101 10 variant variant NN cord-349149-nqsohp9h 101 11 IBV IBV NNP cord-349149-nqsohp9h 101 12 with with IN cord-349149-nqsohp9h 101 13 29 29 CD cord-349149-nqsohp9h 101 14 nucleic nucleic JJ cord-349149-nqsohp9h 101 15 acid acid NN cord-349149-nqsohp9h 101 16 substitutions substitution NNS cord-349149-nqsohp9h 101 17 differing differ VBG cord-349149-nqsohp9h 101 18 from from IN cord-349149-nqsohp9h 101 19 the the DT cord-349149-nqsohp9h 101 20 known know VBN cord-349149-nqsohp9h 101 21 IB IB NNP cord-349149-nqsohp9h 101 22 4/91 4/91 CD cord-349149-nqsohp9h 101 23 vaccine vaccine NN cord-349149-nqsohp9h 101 24 reference reference NN cord-349149-nqsohp9h 101 25 strain strain NN cord-349149-nqsohp9h 101 26 sequence sequence NN cord-349149-nqsohp9h 101 27 , , , cord-349149-nqsohp9h 101 28 which which WDT cord-349149-nqsohp9h 101 29 corresponded correspond VBD cord-349149-nqsohp9h 101 30 to to IN cord-349149-nqsohp9h 101 31 eight eight CD cord-349149-nqsohp9h 101 32 amino amino NN cord-349149-nqsohp9h 101 33 acid acid NN cord-349149-nqsohp9h 101 34 substitutions substitution NNS cord-349149-nqsohp9h 101 35 relative relative JJ cord-349149-nqsohp9h 101 36 to to IN cord-349149-nqsohp9h 101 37 this this DT cord-349149-nqsohp9h 101 38 reference reference NN cord-349149-nqsohp9h 101 39 strain strain NN cord-349149-nqsohp9h 101 40 ( ( -LRB- cord-349149-nqsohp9h 101 41 Table-3 Table-3 NNP cord-349149-nqsohp9h 101 42 ) ) -RRB- cord-349149-nqsohp9h 101 43 . . . cord-349149-nqsohp9h 102 1 Three three CD cord-349149-nqsohp9h 102 2 of of IN cord-349149-nqsohp9h 102 3 these these DT cord-349149-nqsohp9h 102 4 amino amino NN cord-349149-nqsohp9h 102 5 acid acid NN cord-349149-nqsohp9h 102 6 substitutions substitution NNS cord-349149-nqsohp9h 102 7 were be VBD cord-349149-nqsohp9h 102 8 from from IN cord-349149-nqsohp9h 102 9 a a DT cord-349149-nqsohp9h 102 10 hydrophobic hydrophobic NN cord-349149-nqsohp9h 102 11 to to IN cord-349149-nqsohp9h 102 12 a a DT cord-349149-nqsohp9h 102 13 hydrophilic hydrophilic JJ cord-349149-nqsohp9h 102 14 amino amino NN cord-349149-nqsohp9h 102 15 acid acid NN cord-349149-nqsohp9h 102 16 . . . cord-349149-nqsohp9h 103 1 A a DT cord-349149-nqsohp9h 103 2 change change NN cord-349149-nqsohp9h 103 3 in in IN cord-349149-nqsohp9h 103 4 hydrophilicity hydrophilicity NN cord-349149-nqsohp9h 103 5 altered alter VBD cord-349149-nqsohp9h 103 6 the the DT cord-349149-nqsohp9h 103 7 epitopes epitope NNS cord-349149-nqsohp9h 103 8 on on IN cord-349149-nqsohp9h 103 9 the the DT cord-349149-nqsohp9h 103 10 S1 S1 NNP cord-349149-nqsohp9h 103 11 protein protein NN cord-349149-nqsohp9h 103 12 of of IN cord-349149-nqsohp9h 103 13 the the DT cord-349149-nqsohp9h 103 14 IB IB NNP cord-349149-nqsohp9h 103 15 DZ13a DZ13a NNP cord-349149-nqsohp9h 103 16 virus virus NN cord-349149-nqsohp9h 103 17 . . . cord-349149-nqsohp9h 104 1 The the DT cord-349149-nqsohp9h 104 2 predicted predict VBN cord-349149-nqsohp9h 104 3 epitopes epitope NNS cord-349149-nqsohp9h 104 4 with with IN cord-349149-nqsohp9h 104 5 differences difference NNS cord-349149-nqsohp9h 104 6 between between IN cord-349149-nqsohp9h 104 7 the the DT cord-349149-nqsohp9h 104 8 field field NN cord-349149-nqsohp9h 104 9 virus virus NN cord-349149-nqsohp9h 104 10 and and CC cord-349149-nqsohp9h 104 11 IB IB NNP cord-349149-nqsohp9h 104 12 4/91 4/91 CD cord-349149-nqsohp9h 104 13 vaccine vaccine NN cord-349149-nqsohp9h 104 14 strain strain NN cord-349149-nqsohp9h 104 15 are be VBP cord-349149-nqsohp9h 104 16 shown show VBN cord-349149-nqsohp9h 104 17 in in IN cord-349149-nqsohp9h 104 18 Figure- Figure- NNP cord-349149-nqsohp9h 104 19 1 1 CD cord-349149-nqsohp9h 104 20 . . . cord-349149-nqsohp9h 105 1 Infectious infectious JJ cord-349149-nqsohp9h 105 2 bronchitis bronchitis NN cord-349149-nqsohp9h 105 3 has have VBZ cord-349149-nqsohp9h 105 4 had have VBN cord-349149-nqsohp9h 105 5 a a DT cord-349149-nqsohp9h 105 6 devastating devastating JJ cord-349149-nqsohp9h 105 7 effect effect NN cord-349149-nqsohp9h 105 8 on on IN cord-349149-nqsohp9h 105 9 broiler broiler NN cord-349149-nqsohp9h 105 10 farms farm NNS cord-349149-nqsohp9h 105 11 since since IN cord-349149-nqsohp9h 105 12 the the DT cord-349149-nqsohp9h 105 13 worldwide worldwide JJ cord-349149-nqsohp9h 105 14 emergence emergence NN cord-349149-nqsohp9h 105 15 and and CC cord-349149-nqsohp9h 105 16 increased increase VBN cord-349149-nqsohp9h 105 17 prevalence prevalence NN cord-349149-nqsohp9h 105 18 of of IN cord-349149-nqsohp9h 105 19 IBV IBV NNP cord-349149-nqsohp9h 105 20 [ [ -LRB- cord-349149-nqsohp9h 105 21 11 11 CD cord-349149-nqsohp9h 105 22 , , , cord-349149-nqsohp9h 105 23 13 13 CD cord-349149-nqsohp9h 105 24 ] ] -RRB- cord-349149-nqsohp9h 105 25 . . . cord-349149-nqsohp9h 106 1 Due due IN cord-349149-nqsohp9h 106 2 to to IN cord-349149-nqsohp9h 106 3 the the DT cord-349149-nqsohp9h 106 4 high high JJ cord-349149-nqsohp9h 106 5 rate rate NN cord-349149-nqsohp9h 106 6 of of IN cord-349149-nqsohp9h 106 7 mutation mutation NN cord-349149-nqsohp9h 106 8 and and CC cord-349149-nqsohp9h 106 9 recombination recombination NN cord-349149-nqsohp9h 106 10 in in IN cord-349149-nqsohp9h 106 11 coronaviruses coronaviruse NNS cord-349149-nqsohp9h 106 12 , , , cord-349149-nqsohp9h 106 13 new new JJ cord-349149-nqsohp9h 106 14 serotypes serotype NNS cord-349149-nqsohp9h 106 15 and and CC cord-349149-nqsohp9h 106 16 genotypes genotype NNS cord-349149-nqsohp9h 106 17 of of IN cord-349149-nqsohp9h 106 18 IBV IBV NNP cord-349149-nqsohp9h 106 19 frequently frequently RB cord-349149-nqsohp9h 106 20 emerge emerge VBP cord-349149-nqsohp9h 106 21 and and CC cord-349149-nqsohp9h 106 22 have have VBP cord-349149-nqsohp9h 106 23 been be VBN cord-349149-nqsohp9h 106 24 reported report VBN cord-349149-nqsohp9h 106 25 in in IN cord-349149-nqsohp9h 106 26 different different JJ cord-349149-nqsohp9h 106 27 regions region NNS cord-349149-nqsohp9h 106 28 around around IN cord-349149-nqsohp9h 106 29 the the DT cord-349149-nqsohp9h 106 30 world world NN cord-349149-nqsohp9h 106 31 [ [ -LRB- cord-349149-nqsohp9h 106 32 11 11 CD cord-349149-nqsohp9h 106 33 ] ] -RRB- cord-349149-nqsohp9h 106 34 . . . cord-349149-nqsohp9h 107 1 More more RBR cord-349149-nqsohp9h 107 2 recently recently RB cord-349149-nqsohp9h 107 3 , , , cord-349149-nqsohp9h 107 4 IBV IBV NNP cord-349149-nqsohp9h 107 5 has have VBZ cord-349149-nqsohp9h 107 6 been be VBN cord-349149-nqsohp9h 107 7 associated associate VBN cord-349149-nqsohp9h 107 8 with with IN cord-349149-nqsohp9h 107 9 nephritis nephritis NN cord-349149-nqsohp9h 107 10 which which WDT cord-349149-nqsohp9h 107 11 is be VBZ cord-349149-nqsohp9h 107 12 now now RB cord-349149-nqsohp9h 107 13 a a DT cord-349149-nqsohp9h 107 14 major major JJ cord-349149-nqsohp9h 107 15 problem problem NN cord-349149-nqsohp9h 107 16 in in IN cord-349149-nqsohp9h 107 17 broiler broiler NN cord-349149-nqsohp9h 107 18 flocks flock NNS cord-349149-nqsohp9h 107 19 worldwide worldwide RB cord-349149-nqsohp9h 107 20 [ [ -LRB- cord-349149-nqsohp9h 107 21 19 19 CD cord-349149-nqsohp9h 107 22 ] ] -RRB- cord-349149-nqsohp9h 107 23 [ [ -LRB- cord-349149-nqsohp9h 107 24 20 20 CD cord-349149-nqsohp9h 107 25 ] ] -RRB- cord-349149-nqsohp9h 107 26 [ [ -LRB- cord-349149-nqsohp9h 107 27 21 21 CD cord-349149-nqsohp9h 107 28 ] ] -RRB- cord-349149-nqsohp9h 107 29 . . . cord-349149-nqsohp9h 108 1 Similar similar JJ cord-349149-nqsohp9h 108 2 concerns concern NNS cord-349149-nqsohp9h 108 3 have have VBP cord-349149-nqsohp9h 108 4 been be VBN cord-349149-nqsohp9h 108 5 reported report VBN cord-349149-nqsohp9h 108 6 in in IN cord-349149-nqsohp9h 108 7 Algeria Algeria NNP cord-349149-nqsohp9h 108 8 , , , cord-349149-nqsohp9h 108 9 but but CC cord-349149-nqsohp9h 108 10 no no DT cord-349149-nqsohp9h 108 11 study study NN cord-349149-nqsohp9h 108 12 has have VBZ cord-349149-nqsohp9h 108 13 been be VBN cord-349149-nqsohp9h 108 14 performed perform VBN cord-349149-nqsohp9h 108 15 so so RB cord-349149-nqsohp9h 108 16 far far RB cord-349149-nqsohp9h 108 17 on on IN cord-349149-nqsohp9h 108 18 IB IB NNP cord-349149-nqsohp9h 108 19 - - HYPH cord-349149-nqsohp9h 108 20 associated associate VBN cord-349149-nqsohp9h 108 21 nephritis nephritis NN cord-349149-nqsohp9h 108 22 [ [ -LRB- cord-349149-nqsohp9h 108 23 22 22 CD cord-349149-nqsohp9h 108 24 ] ] -RRB- cord-349149-nqsohp9h 108 25 . . . cord-349149-nqsohp9h 108 26 _SP cord-349149-nqsohp9h 109 1 Figure-1 Figure-1 NNP cord-349149-nqsohp9h 109 2 : : : cord-349149-nqsohp9h 109 3 Predicted predict VBN cord-349149-nqsohp9h 109 4 epitope epitope NN cord-349149-nqsohp9h 109 5 alignment alignment NN cord-349149-nqsohp9h 109 6 of of IN cord-349149-nqsohp9h 109 7 deduced deduce VBN cord-349149-nqsohp9h 109 8 amino amino NN cord-349149-nqsohp9h 109 9 sequences sequence NNS cord-349149-nqsohp9h 109 10 of of IN cord-349149-nqsohp9h 109 11 HVR HVR NNP cord-349149-nqsohp9h 109 12 S1 S1 NNP cord-349149-nqsohp9h 109 13 gene gene NN cord-349149-nqsohp9h 109 14 . . . cord-349149-nqsohp9h 110 1 New new JJ cord-349149-nqsohp9h 110 2 field field NN cord-349149-nqsohp9h 110 3 Algerian algerian JJ cord-349149-nqsohp9h 110 4 isolate isolate VBP cord-349149-nqsohp9h 110 5 and and CC cord-349149-nqsohp9h 110 6 selected select VBN cord-349149-nqsohp9h 110 7 infectious infectious JJ cord-349149-nqsohp9h 110 8 bronchitis bronchitis NN cord-349149-nqsohp9h 110 9 virus virus NN cord-349149-nqsohp9h 110 10 vaccine vaccine NN cord-349149-nqsohp9h 110 11 vial vial NN cord-349149-nqsohp9h 110 12 reference reference NN cord-349149-nqsohp9h 110 13 strains strain NNS cord-349149-nqsohp9h 110 14 . . . cord-349149-nqsohp9h 111 1 The the DT cord-349149-nqsohp9h 111 2 present present JJ cord-349149-nqsohp9h 111 3 study study NN cord-349149-nqsohp9h 111 4 was be VBD cord-349149-nqsohp9h 111 5 carried carry VBN cord-349149-nqsohp9h 111 6 out out RP cord-349149-nqsohp9h 111 7 to to TO cord-349149-nqsohp9h 111 8 investigate investigate VB cord-349149-nqsohp9h 111 9 the the DT cord-349149-nqsohp9h 111 10 presence presence NN cord-349149-nqsohp9h 111 11 of of IN cord-349149-nqsohp9h 111 12 IBV IBV NNP cord-349149-nqsohp9h 111 13 in in IN cord-349149-nqsohp9h 111 14 broiler broiler NN cord-349149-nqsohp9h 111 15 flocks flock NNS cord-349149-nqsohp9h 111 16 and and CC cord-349149-nqsohp9h 111 17 its -PRON- PRP$ cord-349149-nqsohp9h 111 18 possible possible JJ cord-349149-nqsohp9h 111 19 involvement involvement NN cord-349149-nqsohp9h 111 20 in in IN cord-349149-nqsohp9h 111 21 kidney kidney NN cord-349149-nqsohp9h 111 22 damage damage NN cord-349149-nqsohp9h 111 23 . . . cord-349149-nqsohp9h 112 1 We -PRON- PRP cord-349149-nqsohp9h 112 2 also also RB cord-349149-nqsohp9h 112 3 monitored monitor VBD cord-349149-nqsohp9h 112 4 the the DT cord-349149-nqsohp9h 112 5 seroprevalence seroprevalence NN cord-349149-nqsohp9h 112 6 of of IN cord-349149-nqsohp9h 112 7 different different JJ cord-349149-nqsohp9h 112 8 IBV IBV NNP cord-349149-nqsohp9h 112 9 serotypes serotype NNS cord-349149-nqsohp9h 112 10 by by IN cord-349149-nqsohp9h 112 11 the the DT cord-349149-nqsohp9h 112 12 HI HI NNP cord-349149-nqsohp9h 112 13 test test NN cord-349149-nqsohp9h 112 14 and and CC cord-349149-nqsohp9h 112 15 analyzed analyze VBD cord-349149-nqsohp9h 112 16 the the DT cord-349149-nqsohp9h 112 17 phylogenetic phylogenetic JJ cord-349149-nqsohp9h 112 18 characteristics characteristic NNS cord-349149-nqsohp9h 112 19 of of IN cord-349149-nqsohp9h 112 20 the the DT cord-349149-nqsohp9h 112 21 new new JJ cord-349149-nqsohp9h 112 22 IBV IBV NNP cord-349149-nqsohp9h 112 23 strains strain NNS cord-349149-nqsohp9h 112 24 isolated isolate VBN cord-349149-nqsohp9h 112 25 in in IN cord-349149-nqsohp9h 112 26 Algeria Algeria NNP cord-349149-nqsohp9h 112 27 in in IN cord-349149-nqsohp9h 112 28 2013 2013 CD cord-349149-nqsohp9h 112 29 by by IN cord-349149-nqsohp9h 112 30 sequencing sequence VBG cord-349149-nqsohp9h 112 31 . . . cord-349149-nqsohp9h 113 1 The the DT cord-349149-nqsohp9h 113 2 HI HI NNP cord-349149-nqsohp9h 113 3 test test NN cord-349149-nqsohp9h 113 4 and and CC cord-349149-nqsohp9h 113 5 virus virus NN cord-349149-nqsohp9h 113 6 neutralization neutralization NN cord-349149-nqsohp9h 113 7 test test NN cord-349149-nqsohp9h 113 8 ( ( -LRB- cord-349149-nqsohp9h 113 9 VNT VNT NNP cord-349149-nqsohp9h 113 10 ) ) -RRB- cord-349149-nqsohp9h 113 11 are be VBP cord-349149-nqsohp9h 113 12 the the DT cord-349149-nqsohp9h 113 13 most most RBS cord-349149-nqsohp9h 113 14 commonly commonly RB cord-349149-nqsohp9h 113 15 used use VBN cord-349149-nqsohp9h 113 16 tests test NNS cord-349149-nqsohp9h 113 17 for for IN cord-349149-nqsohp9h 113 18 detecting detect VBG cord-349149-nqsohp9h 113 19 IBV IBV NNP cord-349149-nqsohp9h 113 20 antibodies antibody NNS cord-349149-nqsohp9h 113 21 [ [ -LRB- cord-349149-nqsohp9h 113 22 9 9 CD cord-349149-nqsohp9h 113 23 , , , cord-349149-nqsohp9h 113 24 23 23 CD cord-349149-nqsohp9h 113 25 ] ] -RRB- cord-349149-nqsohp9h 113 26 . . . cord-349149-nqsohp9h 114 1 Studies study NNS cord-349149-nqsohp9h 114 2 show show VBP cord-349149-nqsohp9h 114 3 extensive extensive JJ cord-349149-nqsohp9h 114 4 cross cross NN cord-349149-nqsohp9h 114 5 - - NNS cord-349149-nqsohp9h 114 6 reactions reaction NNS cord-349149-nqsohp9h 114 7 between between IN cord-349149-nqsohp9h 114 8 the the DT cord-349149-nqsohp9h 114 9 IBV IBV NNP cord-349149-nqsohp9h 114 10 serotypes serotype NNS cord-349149-nqsohp9h 114 11 detected detect VBN cord-349149-nqsohp9h 114 12 by by IN cord-349149-nqsohp9h 114 13 the the DT cord-349149-nqsohp9h 114 14 HI HI NNP cord-349149-nqsohp9h 114 15 test test NN cord-349149-nqsohp9h 114 16 and and CC cord-349149-nqsohp9h 114 17 similar similar JJ cord-349149-nqsohp9h 114 18 antigenicity antigenicity NN cord-349149-nqsohp9h 114 19 detected detect VBN cord-349149-nqsohp9h 114 20 by by IN cord-349149-nqsohp9h 114 21 the the DT cord-349149-nqsohp9h 114 22 VNT VNT NNP cord-349149-nqsohp9h 114 23 and and CC cord-349149-nqsohp9h 114 24 HI HI NNP cord-349149-nqsohp9h 114 25 test test VBP cord-349149-nqsohp9h 114 26 [ [ -LRB- cord-349149-nqsohp9h 114 27 24 24 CD cord-349149-nqsohp9h 114 28 ] ] -RRB- cord-349149-nqsohp9h 115 1 [ [ -LRB- cord-349149-nqsohp9h 115 2 25 25 CD cord-349149-nqsohp9h 115 3 ] ] -RRB- cord-349149-nqsohp9h 115 4 [ [ -LRB- cord-349149-nqsohp9h 115 5 26 26 CD cord-349149-nqsohp9h 115 6 ] ] -RRB- cord-349149-nqsohp9h 115 7 . . . cord-349149-nqsohp9h 116 1 However however RB cord-349149-nqsohp9h 116 2 , , , cord-349149-nqsohp9h 116 3 since since IN cord-349149-nqsohp9h 116 4 VNTs vnts NN cord-349149-nqsohp9h 116 5 are be VBP cord-349149-nqsohp9h 116 6 not not RB cord-349149-nqsohp9h 116 7 cost cost NN cord-349149-nqsohp9h 116 8 - - HYPH cord-349149-nqsohp9h 116 9 effective effective JJ cord-349149-nqsohp9h 116 10 for for IN cord-349149-nqsohp9h 116 11 routine routine JJ cord-349149-nqsohp9h 116 12 examination examination NN cord-349149-nqsohp9h 116 13 , , , cord-349149-nqsohp9h 116 14 HI HI NNP cord-349149-nqsohp9h 116 15 tests test NNS cord-349149-nqsohp9h 116 16 are be VBP cord-349149-nqsohp9h 116 17 commonly commonly RB cord-349149-nqsohp9h 116 18 used use VBN cord-349149-nqsohp9h 116 19 for for IN cord-349149-nqsohp9h 116 20 routine routine JJ cord-349149-nqsohp9h 116 21 serology serology NN cord-349149-nqsohp9h 116 22 [ [ -LRB- cord-349149-nqsohp9h 116 23 27 27 CD cord-349149-nqsohp9h 116 24 ] ] -RRB- cord-349149-nqsohp9h 116 25 . . . cord-349149-nqsohp9h 117 1 The the DT cord-349149-nqsohp9h 117 2 most most RBS cord-349149-nqsohp9h 117 3 frequently frequently RB cord-349149-nqsohp9h 117 4 detected detect VBD cord-349149-nqsohp9h 117 5 IBV IBV NNP cord-349149-nqsohp9h 117 6 serotypes serotype NNS cord-349149-nqsohp9h 117 7 in in IN cord-349149-nqsohp9h 117 8 the the DT cord-349149-nqsohp9h 117 9 broiler broiler NN cord-349149-nqsohp9h 117 10 flocks flock NNS cord-349149-nqsohp9h 117 11 with with IN cord-349149-nqsohp9h 117 12 nephritis nephritis NN cord-349149-nqsohp9h 117 13 were be VBD cord-349149-nqsohp9h 117 14 IB IB NNP cord-349149-nqsohp9h 117 15 - - HYPH cord-349149-nqsohp9h 117 16 QX QX NNP cord-349149-nqsohp9h 117 17 ( ( -LRB- cord-349149-nqsohp9h 117 18 100 100 CD cord-349149-nqsohp9h 117 19 % % NN cord-349149-nqsohp9h 117 20 ) ) -RRB- cord-349149-nqsohp9h 117 21 and and CC cord-349149-nqsohp9h 117 22 IB IB NNP cord-349149-nqsohp9h 117 23 4/91 4/91 CD cord-349149-nqsohp9h 117 24 ( ( -LRB- cord-349149-nqsohp9h 117 25 60 60 CD cord-349149-nqsohp9h 117 26 % % NN cord-349149-nqsohp9h 117 27 ) ) -RRB- cord-349149-nqsohp9h 117 28 , , , cord-349149-nqsohp9h 117 29 which which WDT cord-349149-nqsohp9h 117 30 have have VBP cord-349149-nqsohp9h 117 31 been be VBN cord-349149-nqsohp9h 117 32 detected detect VBN cord-349149-nqsohp9h 117 33 for for IN cord-349149-nqsohp9h 117 34 the the DT cord-349149-nqsohp9h 117 35 1 1 CD cord-349149-nqsohp9h 117 36 st st NNP cord-349149-nqsohp9h 117 37 time time NN cord-349149-nqsohp9h 117 38 in in IN cord-349149-nqsohp9h 117 39 Algeria Algeria NNP cord-349149-nqsohp9h 117 40 by by IN cord-349149-nqsohp9h 117 41 our -PRON- PRP$ cord-349149-nqsohp9h 117 42 group group NN cord-349149-nqsohp9h 117 43 . . . cord-349149-nqsohp9h 118 1 After after IN cord-349149-nqsohp9h 118 2 reporting report VBG cord-349149-nqsohp9h 118 3 the the DT cord-349149-nqsohp9h 118 4 nephropathogenic nephropathogenic JJ cord-349149-nqsohp9h 118 5 form form NN cord-349149-nqsohp9h 118 6 of of IN cord-349149-nqsohp9h 118 7 IB IB NNP cord-349149-nqsohp9h 118 8 [ [ -LRB- cord-349149-nqsohp9h 118 9 3 3 CD cord-349149-nqsohp9h 118 10 ] ] -RRB- cord-349149-nqsohp9h 118 11 , , , cord-349149-nqsohp9h 118 12 the the DT cord-349149-nqsohp9h 118 13 above above JJ cord-349149-nqsohp9h 118 14 serotypes serotype NNS cord-349149-nqsohp9h 118 15 have have VBP cord-349149-nqsohp9h 118 16 been be VBN cord-349149-nqsohp9h 118 17 reported report VBN cord-349149-nqsohp9h 118 18 in in IN cord-349149-nqsohp9h 118 19 China China NNP cord-349149-nqsohp9h 118 20 , , , cord-349149-nqsohp9h 118 21 Europe Europe NNP cord-349149-nqsohp9h 118 22 , , , cord-349149-nqsohp9h 118 23 and and CC cord-349149-nqsohp9h 118 24 Africa Africa NNP cord-349149-nqsohp9h 118 25 and and CC cord-349149-nqsohp9h 118 26 have have VBP cord-349149-nqsohp9h 118 27 been be VBN cord-349149-nqsohp9h 118 28 seen see VBN cord-349149-nqsohp9h 118 29 to to TO cord-349149-nqsohp9h 118 30 be be VB cord-349149-nqsohp9h 118 31 associated associate VBN cord-349149-nqsohp9h 118 32 with with IN cord-349149-nqsohp9h 118 33 severe severe JJ cord-349149-nqsohp9h 118 34 kidney kidney NN cord-349149-nqsohp9h 118 35 damage damage NN cord-349149-nqsohp9h 118 36 , , , cord-349149-nqsohp9h 118 37 high high JJ cord-349149-nqsohp9h 118 38 morbidity morbidity NN cord-349149-nqsohp9h 118 39 , , , cord-349149-nqsohp9h 118 40 and and CC cord-349149-nqsohp9h 118 41 mortality mortality NN cord-349149-nqsohp9h 118 42 in in IN cord-349149-nqsohp9h 118 43 the the DT cord-349149-nqsohp9h 118 44 vaccinated vaccinate VBN cord-349149-nqsohp9h 118 45 flocks flock NNS cord-349149-nqsohp9h 118 46 . . . cord-349149-nqsohp9h 119 1 At at IN cord-349149-nqsohp9h 119 2 present present NN cord-349149-nqsohp9h 119 3 , , , cord-349149-nqsohp9h 119 4 these these DT cord-349149-nqsohp9h 119 5 serotypes serotype NNS cord-349149-nqsohp9h 119 6 are be VBP cord-349149-nqsohp9h 119 7 the the DT cord-349149-nqsohp9h 119 8 predominant predominant JJ cord-349149-nqsohp9h 119 9 field field NN cord-349149-nqsohp9h 119 10 strains strain NNS cord-349149-nqsohp9h 119 11 in in IN cord-349149-nqsohp9h 119 12 most most JJS cord-349149-nqsohp9h 119 13 poultry poultry NN cord-349149-nqsohp9h 119 14 farms farm NNS cord-349149-nqsohp9h 119 15 worldwide worldwide RB cord-349149-nqsohp9h 119 16 [ [ -LRB- cord-349149-nqsohp9h 119 17 13 13 CD cord-349149-nqsohp9h 119 18 , , , cord-349149-nqsohp9h 119 19 28 28 CD cord-349149-nqsohp9h 119 20 ] ] -RRB- cord-349149-nqsohp9h 119 21 . . . cord-349149-nqsohp9h 120 1 The the DT cord-349149-nqsohp9h 120 2 serological serological JJ cord-349149-nqsohp9h 120 3 analysis analysis NN cord-349149-nqsohp9h 120 4 showed show VBD cord-349149-nqsohp9h 120 5 significantly significantly RB cord-349149-nqsohp9h 120 6 lower low JJR cord-349149-nqsohp9h 120 7 titers titer NNS cord-349149-nqsohp9h 120 8 of of IN cord-349149-nqsohp9h 120 9 IB IB NNP cord-349149-nqsohp9h 120 10 4/91 4/91 CD cord-349149-nqsohp9h 120 11 compared compare VBN cord-349149-nqsohp9h 120 12 to to IN cord-349149-nqsohp9h 120 13 IB IB NNP cord-349149-nqsohp9h 120 14 - - HYPH cord-349149-nqsohp9h 120 15 QX QX NNP cord-349149-nqsohp9h 120 16 , , , cord-349149-nqsohp9h 120 17 which which WDT cord-349149-nqsohp9h 120 18 could could MD cord-349149-nqsohp9h 120 19 be be VB cord-349149-nqsohp9h 120 20 related relate VBN cord-349149-nqsohp9h 120 21 to to IN cord-349149-nqsohp9h 120 22 the the DT cord-349149-nqsohp9h 120 23 vaccination vaccination NN cord-349149-nqsohp9h 120 24 of of IN cord-349149-nqsohp9h 120 25 some some DT cord-349149-nqsohp9h 120 26 flocks flock NNS cord-349149-nqsohp9h 120 27 with with IN cord-349149-nqsohp9h 120 28 the the DT cord-349149-nqsohp9h 120 29 IB IB NNP cord-349149-nqsohp9h 120 30 4/91 4/91 CD cord-349149-nqsohp9h 120 31 strain strain NN cord-349149-nqsohp9h 120 32 . . . cord-349149-nqsohp9h 121 1 De De NNP cord-349149-nqsohp9h 121 2 Wit Wit NNP cord-349149-nqsohp9h 121 3 [ [ -LRB- cord-349149-nqsohp9h 121 4 23 23 CD cord-349149-nqsohp9h 121 5 ] ] -RRB- cord-349149-nqsohp9h 121 6 reported report VBD cord-349149-nqsohp9h 121 7 that that IN cord-349149-nqsohp9h 121 8 many many JJ cord-349149-nqsohp9h 121 9 factors factor NNS cord-349149-nqsohp9h 121 10 influence influence VBP cord-349149-nqsohp9h 121 11 the the DT cord-349149-nqsohp9h 121 12 level level NN cord-349149-nqsohp9h 121 13 of of IN cord-349149-nqsohp9h 121 14 IBV IBV NNP cord-349149-nqsohp9h 121 15 antibodies antibody NNS cord-349149-nqsohp9h 121 16 , , , cord-349149-nqsohp9h 121 17 of of IN cord-349149-nqsohp9h 121 18 which which WDT cord-349149-nqsohp9h 121 19 the the DT cord-349149-nqsohp9h 121 20 most most RBS cord-349149-nqsohp9h 121 21 critical critical JJ cord-349149-nqsohp9h 121 22 is be VBZ cord-349149-nqsohp9h 121 23 the the DT cord-349149-nqsohp9h 121 24 presence presence NN cord-349149-nqsohp9h 121 25 of of IN cord-349149-nqsohp9h 121 26 immunity immunity NN cord-349149-nqsohp9h 121 27 at at IN cord-349149-nqsohp9h 121 28 the the DT cord-349149-nqsohp9h 121 29 time time NN cord-349149-nqsohp9h 121 30 of of IN cord-349149-nqsohp9h 121 31 infection infection NN cord-349149-nqsohp9h 121 32 . . . cord-349149-nqsohp9h 122 1 This this DT cord-349149-nqsohp9h 122 2 is be VBZ cord-349149-nqsohp9h 122 3 consistent consistent JJ cord-349149-nqsohp9h 122 4 with with IN cord-349149-nqsohp9h 122 5 the the DT cord-349149-nqsohp9h 122 6 lower low JJR cord-349149-nqsohp9h 122 7 anti anti JJ cord-349149-nqsohp9h 122 8 - - JJ cord-349149-nqsohp9h 122 9 IB IB NNP cord-349149-nqsohp9h 122 10 4/91 4/91 CD cord-349149-nqsohp9h 122 11 antibody antibody NN cord-349149-nqsohp9h 122 12 titers titer NNS cord-349149-nqsohp9h 122 13 seen see VBN cord-349149-nqsohp9h 122 14 in in IN cord-349149-nqsohp9h 122 15 the the DT cord-349149-nqsohp9h 122 16 vaccinated vaccinate VBN cord-349149-nqsohp9h 122 17 flocks flock NNS cord-349149-nqsohp9h 122 18 compared compare VBN cord-349149-nqsohp9h 122 19 to to IN cord-349149-nqsohp9h 122 20 the the DT cord-349149-nqsohp9h 122 21 unvaccinated unvaccinated JJ cord-349149-nqsohp9h 122 22 ones one NNS cord-349149-nqsohp9h 122 23 . . . cord-349149-nqsohp9h 123 1 Another another DT cord-349149-nqsohp9h 123 2 factor factor NN cord-349149-nqsohp9h 123 3 that that WDT cord-349149-nqsohp9h 123 4 can can MD cord-349149-nqsohp9h 123 5 reduce reduce VB cord-349149-nqsohp9h 123 6 the the DT cord-349149-nqsohp9h 123 7 sensitivity sensitivity NN cord-349149-nqsohp9h 123 8 of of IN cord-349149-nqsohp9h 123 9 HI HI NNP cord-349149-nqsohp9h 123 10 and and CC cord-349149-nqsohp9h 123 11 thus thus RB cord-349149-nqsohp9h 123 12 affect affect VBP cord-349149-nqsohp9h 123 13 the the DT cord-349149-nqsohp9h 123 14 interpretation interpretation NN cord-349149-nqsohp9h 123 15 of of IN cord-349149-nqsohp9h 123 16 the the DT cord-349149-nqsohp9h 123 17 results result NNS cord-349149-nqsohp9h 123 18 is be VBZ cord-349149-nqsohp9h 123 19 the the DT cord-349149-nqsohp9h 123 20 cross cross NN cord-349149-nqsohp9h 123 21 - - NN cord-349149-nqsohp9h 123 22 reaction reaction NN cord-349149-nqsohp9h 123 23 between between IN cord-349149-nqsohp9h 123 24 the the DT cord-349149-nqsohp9h 123 25 different different JJ cord-349149-nqsohp9h 123 26 serotypes serotype NNS cord-349149-nqsohp9h 123 27 , , , cord-349149-nqsohp9h 123 28 especially especially RB cord-349149-nqsohp9h 123 29 when when WRB cord-349149-nqsohp9h 123 30 multiple multiple JJ cord-349149-nqsohp9h 123 31 serotypes serotype NNS cord-349149-nqsohp9h 123 32 of of IN cord-349149-nqsohp9h 123 33 IBV IBV NNP cord-349149-nqsohp9h 123 34 are be VBP cord-349149-nqsohp9h 123 35 involved involve VBN cord-349149-nqsohp9h 123 36 . . . cord-349149-nqsohp9h 124 1 We -PRON- PRP cord-349149-nqsohp9h 124 2 detected detect VBD cord-349149-nqsohp9h 124 3 cross cross JJ cord-349149-nqsohp9h 124 4 - - NN cord-349149-nqsohp9h 124 5 reaction reaction NN cord-349149-nqsohp9h 124 6 between between IN cord-349149-nqsohp9h 124 7 the the DT cord-349149-nqsohp9h 124 8 QX QX NNP cord-349149-nqsohp9h 124 9 and and CC cord-349149-nqsohp9h 124 10 4/91 4/91 CD cord-349149-nqsohp9h 124 11 serotypes serotype NNS cord-349149-nqsohp9h 124 12 in in IN cord-349149-nqsohp9h 124 13 many many JJ cord-349149-nqsohp9h 124 14 flocks flock NNS cord-349149-nqsohp9h 124 15 , , , cord-349149-nqsohp9h 124 16 and and CC cord-349149-nqsohp9h 124 17 4 4 CD cord-349149-nqsohp9h 124 18 flocks flock NNS cord-349149-nqsohp9h 124 19 showed show VBD cord-349149-nqsohp9h 124 20 mixed mixed JJ cord-349149-nqsohp9h 124 21 infection infection NN cord-349149-nqsohp9h 124 22 of of IN cord-349149-nqsohp9h 124 23 three three CD cord-349149-nqsohp9h 124 24 serotypes serotype NNS cord-349149-nqsohp9h 124 25 ( ( -LRB- cord-349149-nqsohp9h 124 26 QX QX NNP cord-349149-nqsohp9h 124 27 , , , cord-349149-nqsohp9h 124 28 4/91 4/91 CD cord-349149-nqsohp9h 124 29 , , , cord-349149-nqsohp9h 124 30 and and CC cord-349149-nqsohp9h 124 31 M41 M41 NNP cord-349149-nqsohp9h 124 32 ) ) -RRB- cord-349149-nqsohp9h 124 33 . . . cord-349149-nqsohp9h 125 1 Therefore therefore RB cord-349149-nqsohp9h 125 2 , , , cord-349149-nqsohp9h 125 3 the the DT cord-349149-nqsohp9h 125 4 serotype serotype NN cord-349149-nqsohp9h 125 5 specificity specificity NN cord-349149-nqsohp9h 125 6 of of IN cord-349149-nqsohp9h 125 7 HI HI NNP cord-349149-nqsohp9h 125 8 is be VBZ cord-349149-nqsohp9h 125 9 reduced reduce VBN cord-349149-nqsohp9h 125 10 after after IN cord-349149-nqsohp9h 125 11 reinfection reinfection NN cord-349149-nqsohp9h 125 12 or or CC cord-349149-nqsohp9h 125 13 vaccination vaccination NN cord-349149-nqsohp9h 125 14 , , , cord-349149-nqsohp9h 125 15 especially especially RB cord-349149-nqsohp9h 125 16 when when WRB cord-349149-nqsohp9h 125 17 the the DT cord-349149-nqsohp9h 125 18 subsequent subsequent JJ cord-349149-nqsohp9h 125 19 serotype serotype NN cord-349149-nqsohp9h 125 20 is be VBZ cord-349149-nqsohp9h 125 21 heterologous heterologous JJ cord-349149-nqsohp9h 125 22 [ [ -LRB- cord-349149-nqsohp9h 125 23 23 23 CD cord-349149-nqsohp9h 125 24 ] ] -RRB- cord-349149-nqsohp9h 125 25 . . . cord-349149-nqsohp9h 126 1 The the DT cord-349149-nqsohp9h 126 2 S1 s1 NN cord-349149-nqsohp9h 126 3 protein protein NN cord-349149-nqsohp9h 126 4 of of IN cord-349149-nqsohp9h 126 5 IBV IBV NNP cord-349149-nqsohp9h 126 6 determines determine VBZ cord-349149-nqsohp9h 126 7 the the DT cord-349149-nqsohp9h 126 8 genotype genotype NN cord-349149-nqsohp9h 126 9 , , , cord-349149-nqsohp9h 126 10 serotype serotype NN cord-349149-nqsohp9h 126 11 , , , cord-349149-nqsohp9h 126 12 and and CC cord-349149-nqsohp9h 126 13 phenotype phenotype NN cord-349149-nqsohp9h 126 14 of of IN cord-349149-nqsohp9h 126 15 IBVs ibv NNS cord-349149-nqsohp9h 126 16 [ [ -LRB- cord-349149-nqsohp9h 126 17 29 29 CD cord-349149-nqsohp9h 126 18 ] ] -RRB- cord-349149-nqsohp9h 126 19 . . . cord-349149-nqsohp9h 127 1 The the DT cord-349149-nqsohp9h 127 2 S1 s1 NN cord-349149-nqsohp9h 127 3 gene gene NN cord-349149-nqsohp9h 127 4 of of IN cord-349149-nqsohp9h 127 5 the the DT cord-349149-nqsohp9h 127 6 IBDZ13a ibdz13a JJ cord-349149-nqsohp9h 127 7 field field NN cord-349149-nqsohp9h 127 8 strain strain NN cord-349149-nqsohp9h 127 9 was be VBD cord-349149-nqsohp9h 127 10 most most RBS cord-349149-nqsohp9h 127 11 closely closely RB cord-349149-nqsohp9h 127 12 related relate VBN cord-349149-nqsohp9h 127 13 to to IN cord-349149-nqsohp9h 127 14 that that DT cord-349149-nqsohp9h 127 15 of of IN cord-349149-nqsohp9h 127 16 the the DT cord-349149-nqsohp9h 127 17 IB IB NNP cord-349149-nqsohp9h 127 18 4/91-type 4/91-type CD cord-349149-nqsohp9h 127 19 strain strain NN cord-349149-nqsohp9h 127 20 ( ( -LRB- cord-349149-nqsohp9h 127 21 Figure-2 Figure-2 NNP cord-349149-nqsohp9h 127 22 ) ) -RRB- cord-349149-nqsohp9h 127 23 as as IN cord-349149-nqsohp9h 127 24 determined determine VBN cord-349149-nqsohp9h 127 25 by by IN cord-349149-nqsohp9h 127 26 the the DT cord-349149-nqsohp9h 127 27 criterion criterion NN cord-349149-nqsohp9h 127 28 of of IN cord-349149-nqsohp9h 127 29 Worthington Worthington NNP cord-349149-nqsohp9h 127 30 et et NNP cord-349149-nqsohp9h 127 31 al al NNP cord-349149-nqsohp9h 127 32 . . . cord-349149-nqsohp9h 128 1 [ [ -LRB- cord-349149-nqsohp9h 128 2 13 13 CD cord-349149-nqsohp9h 128 3 ] ] -RRB- cord-349149-nqsohp9h 128 4 and and CC cord-349149-nqsohp9h 128 5 Kannan Kannan NNP cord-349149-nqsohp9h 128 6 et et NNP cord-349149-nqsohp9h 128 7 al al NNP cord-349149-nqsohp9h 128 8 . . . cord-349149-nqsohp9h 129 1 [ [ -LRB- cord-349149-nqsohp9h 129 2 30 30 CD cord-349149-nqsohp9h 129 3 ] ] -RRB- cord-349149-nqsohp9h 129 4 , , , cord-349149-nqsohp9h 129 5 who who WP cord-349149-nqsohp9h 129 6 classified classify VBD cord-349149-nqsohp9h 129 7 IBV IBV NNP cord-349149-nqsohp9h 129 8 sequences sequence NNS cord-349149-nqsohp9h 129 9 with with IN cord-349149-nqsohp9h 129 10 < < XX cord-349149-nqsohp9h 129 11 99 99 CD cord-349149-nqsohp9h 129 12 % % NN cord-349149-nqsohp9h 129 13 homology homology NN cord-349149-nqsohp9h 129 14 to to IN cord-349149-nqsohp9h 129 15 vaccine vaccine NN cord-349149-nqsohp9h 129 16 strains strain NNS cord-349149-nqsohp9h 129 17 as as IN cord-349149-nqsohp9h 129 18 variants variant NNS cord-349149-nqsohp9h 129 19 . . . cord-349149-nqsohp9h 130 1 Our -PRON- PRP$ cord-349149-nqsohp9h 130 2 findings finding NNS cord-349149-nqsohp9h 130 3 indicate indicate VBP cord-349149-nqsohp9h 130 4 the the DT cord-349149-nqsohp9h 130 5 circulation circulation NN cord-349149-nqsohp9h 130 6 of of IN cord-349149-nqsohp9h 130 7 virulent virulent JJ cord-349149-nqsohp9h 130 8 strains strain NNS cord-349149-nqsohp9h 130 9 of of IN cord-349149-nqsohp9h 130 10 IB IB NNP cord-349149-nqsohp9h 130 11 4/91 4/91 CD cord-349149-nqsohp9h 130 12 , , , cord-349149-nqsohp9h 130 13 which which WDT cord-349149-nqsohp9h 130 14 have have VBP cord-349149-nqsohp9h 130 15 become become VBN cord-349149-nqsohp9h 130 16 the the DT cord-349149-nqsohp9h 130 17 predominant predominant JJ cord-349149-nqsohp9h 130 18 genotype genotype NN cord-349149-nqsohp9h 130 19 in in IN cord-349149-nqsohp9h 130 20 Africa Africa NNP cord-349149-nqsohp9h 130 21 , , , cord-349149-nqsohp9h 130 22 Asia Asia NNP cord-349149-nqsohp9h 130 23 , , , cord-349149-nqsohp9h 130 24 and and CC cord-349149-nqsohp9h 130 25 Europe Europe NNP cord-349149-nqsohp9h 130 26 [ [ -LRB- cord-349149-nqsohp9h 130 27 28 28 CD cord-349149-nqsohp9h 130 28 ] ] -RRB- cord-349149-nqsohp9h 130 29 . . . cord-349149-nqsohp9h 131 1 However however RB cord-349149-nqsohp9h 131 2 , , , cord-349149-nqsohp9h 131 3 we -PRON- PRP cord-349149-nqsohp9h 131 4 did do VBD cord-349149-nqsohp9h 131 5 not not RB cord-349149-nqsohp9h 131 6 see see VB cord-349149-nqsohp9h 131 7 a a DT cord-349149-nqsohp9h 131 8 strong strong JJ cord-349149-nqsohp9h 131 9 correlation correlation NN cord-349149-nqsohp9h 131 10 between between IN cord-349149-nqsohp9h 131 11 the the DT cord-349149-nqsohp9h 131 12 HI HI NNP cord-349149-nqsohp9h 131 13 results result NNS cord-349149-nqsohp9h 131 14 which which WDT cord-349149-nqsohp9h 131 15 showed show VBD cord-349149-nqsohp9h 131 16 the the DT cord-349149-nqsohp9h 131 17 circulation circulation NN cord-349149-nqsohp9h 131 18 of of IN cord-349149-nqsohp9h 131 19 the the DT cord-349149-nqsohp9h 131 20 QX QX NNP cord-349149-nqsohp9h 131 21 and and CC cord-349149-nqsohp9h 131 22 4/91 4/91 CD cord-349149-nqsohp9h 131 23 serotypes serotype NNS cord-349149-nqsohp9h 131 24 and and CC cord-349149-nqsohp9h 131 25 RT RT NNP cord-349149-nqsohp9h 131 26 - - HYPH cord-349149-nqsohp9h 131 27 PCR PCR NNP cord-349149-nqsohp9h 131 28 which which WDT cord-349149-nqsohp9h 131 29 showed show VBD cord-349149-nqsohp9h 131 30 the the DT cord-349149-nqsohp9h 131 31 emergence emergence NN cord-349149-nqsohp9h 131 32 of of IN cord-349149-nqsohp9h 131 33 a a DT cord-349149-nqsohp9h 131 34 new new JJ cord-349149-nqsohp9h 131 35 genotype genotype NN cord-349149-nqsohp9h 131 36 of of IN cord-349149-nqsohp9h 131 37 the the DT cord-349149-nqsohp9h 131 38 4/91 4/91 CD cord-349149-nqsohp9h 131 39 strain strain NN cord-349149-nqsohp9h 131 40 . . . cord-349149-nqsohp9h 132 1 This this DT cord-349149-nqsohp9h 132 2 is be VBZ cord-349149-nqsohp9h 132 3 consistent consistent JJ cord-349149-nqsohp9h 132 4 with with IN cord-349149-nqsohp9h 132 5 the the DT cord-349149-nqsohp9h 132 6 findings finding NNS cord-349149-nqsohp9h 132 7 of of IN cord-349149-nqsohp9h 132 8 Kusters Kusters NNPS cord-349149-nqsohp9h 132 9 et et NNP cord-349149-nqsohp9h 132 10 al al NNP cord-349149-nqsohp9h 132 11 . . . cord-349149-nqsohp9h 133 1 [ [ -LRB- cord-349149-nqsohp9h 133 2 31 31 CD cord-349149-nqsohp9h 133 3 ] ] -RRB- cord-349149-nqsohp9h 133 4 who who WP cord-349149-nqsohp9h 133 5 also also RB cord-349149-nqsohp9h 133 6 reported report VBD cord-349149-nqsohp9h 133 7 that that IN cord-349149-nqsohp9h 133 8 RT RT NNP cord-349149-nqsohp9h 133 9 - - HYPH cord-349149-nqsohp9h 133 10 PCR PCR NNP cord-349149-nqsohp9h 133 11 and and CC cord-349149-nqsohp9h 133 12 HI HI NNP cord-349149-nqsohp9h 133 13 results result NNS cord-349149-nqsohp9h 133 14 were be VBD cord-349149-nqsohp9h 133 15 not not RB cord-349149-nqsohp9h 133 16 exactly exactly RB cord-349149-nqsohp9h 133 17 correlated correlate VBN cord-349149-nqsohp9h 133 18 , , , cord-349149-nqsohp9h 133 19 wherein wherein WRB cord-349149-nqsohp9h 133 20 different different JJ cord-349149-nqsohp9h 133 21 serotypes serotype NNS cord-349149-nqsohp9h 133 22 showed show VBD cord-349149-nqsohp9h 133 23 considerable considerable JJ cord-349149-nqsohp9h 133 24 differences difference NNS cord-349149-nqsohp9h 133 25 ( ( -LRB- cord-349149-nqsohp9h 133 26 20 20 CD cord-349149-nqsohp9h 133 27 - - SYM cord-349149-nqsohp9h 133 28 50 50 CD cord-349149-nqsohp9h 133 29 % % NN cord-349149-nqsohp9h 133 30 ) ) -RRB- cord-349149-nqsohp9h 133 31 in in IN cord-349149-nqsohp9h 133 32 the the DT cord-349149-nqsohp9h 133 33 amino amino NN cord-349149-nqsohp9h 133 34 acid acid NN cord-349149-nqsohp9h 133 35 sequences sequence NNS cord-349149-nqsohp9h 133 36 of of IN cord-349149-nqsohp9h 133 37 the the DT cord-349149-nqsohp9h 133 38 S1 S1 NNP cord-349149-nqsohp9h 133 39 subunit subunit NN cord-349149-nqsohp9h 133 40 . . . cord-349149-nqsohp9h 134 1 HI HI NNP cord-349149-nqsohp9h 134 2 is be VBZ cord-349149-nqsohp9h 134 3 used use VBN cord-349149-nqsohp9h 134 4 for for IN cord-349149-nqsohp9h 134 5 routine routine JJ cord-349149-nqsohp9h 134 6 testing testing NN cord-349149-nqsohp9h 134 7 of of IN cord-349149-nqsohp9h 134 8 sera sera NNP cord-349149-nqsohp9h 134 9 from from IN cord-349149-nqsohp9h 134 10 young young JJ cord-349149-nqsohp9h 134 11 growing grow VBG cord-349149-nqsohp9h 134 12 chickens chicken NNS cord-349149-nqsohp9h 134 13 to to TO cord-349149-nqsohp9h 134 14 determine determine VB cord-349149-nqsohp9h 134 15 the the DT cord-349149-nqsohp9h 134 16 serotype serotype NN cord-349149-nqsohp9h 134 17 - - HYPH cord-349149-nqsohp9h 134 18 specific specific JJ cord-349149-nqsohp9h 134 19 antibody antibody NN cord-349149-nqsohp9h 134 20 status status NN cord-349149-nqsohp9h 134 21 of of IN cord-349149-nqsohp9h 134 22 a a DT cord-349149-nqsohp9h 134 23 flock flock NN cord-349149-nqsohp9h 134 24 [ [ -LRB- cord-349149-nqsohp9h 134 25 32 32 CD cord-349149-nqsohp9h 134 26 ] ] -RRB- cord-349149-nqsohp9h 134 27 . . . cord-349149-nqsohp9h 135 1 However however RB cord-349149-nqsohp9h 135 2 , , , cord-349149-nqsohp9h 135 3 the the DT cord-349149-nqsohp9h 135 4 presence presence NN cord-349149-nqsohp9h 135 5 of of IN cord-349149-nqsohp9h 135 6 multiple multiple JJ cord-349149-nqsohp9h 135 7 cross cross JJ cord-349149-nqsohp9h 135 8 - - JJ cord-349149-nqsohp9h 135 9 reactive reactive JJ cord-349149-nqsohp9h 135 10 antibodies antibody NNS cord-349149-nqsohp9h 135 11 against against IN cord-349149-nqsohp9h 135 12 antigenically antigenically RB cord-349149-nqsohp9h 135 13 unrelated unrelated JJ cord-349149-nqsohp9h 135 14 strains strain NNS cord-349149-nqsohp9h 135 15 , , , cord-349149-nqsohp9h 135 16 as as IN cord-349149-nqsohp9h 135 17 is be VBZ cord-349149-nqsohp9h 135 18 often often RB cord-349149-nqsohp9h 135 19 seen see VBN cord-349149-nqsohp9h 135 20 during during IN cord-349149-nqsohp9h 135 21 IB IB NNP cord-349149-nqsohp9h 135 22 outbreaks outbreak NNS cord-349149-nqsohp9h 135 23 , , , cord-349149-nqsohp9h 135 24 reduces reduce VBZ cord-349149-nqsohp9h 135 25 the the DT cord-349149-nqsohp9h 135 26 serodiagnostic serodiagnostic JJ cord-349149-nqsohp9h 135 27 value value NN cord-349149-nqsohp9h 135 28 of of IN cord-349149-nqsohp9h 135 29 HI HI NNP cord-349149-nqsohp9h 135 30 . . . cord-349149-nqsohp9h 136 1 Therefore therefore RB cord-349149-nqsohp9h 136 2 , , , cord-349149-nqsohp9h 136 3 a a DT cord-349149-nqsohp9h 136 4 combination combination NN cord-349149-nqsohp9h 136 5 of of IN cord-349149-nqsohp9h 136 6 serotyping serotype VBG cord-349149-nqsohp9h 136 7 and and CC cord-349149-nqsohp9h 136 8 genotyping genotyping NN cord-349149-nqsohp9h 136 9 is be VBZ cord-349149-nqsohp9h 136 10 recommended recommend VBN cord-349149-nqsohp9h 136 11 to to TO cord-349149-nqsohp9h 136 12 identify identify VB cord-349149-nqsohp9h 136 13 field field NN cord-349149-nqsohp9h 136 14 strains strain NNS cord-349149-nqsohp9h 136 15 . . . cord-349149-nqsohp9h 137 1 Based base VBN cord-349149-nqsohp9h 137 2 on on IN cord-349149-nqsohp9h 137 3 the the DT cord-349149-nqsohp9h 137 4 results result NNS cord-349149-nqsohp9h 137 5 of of IN cord-349149-nqsohp9h 137 6 HI HI NNP cord-349149-nqsohp9h 137 7 and and CC cord-349149-nqsohp9h 137 8 RT RT NNP cord-349149-nqsohp9h 137 9 - - HYPH cord-349149-nqsohp9h 137 10 PCR PCR NNP cord-349149-nqsohp9h 137 11 , , , cord-349149-nqsohp9h 137 12 we -PRON- PRP cord-349149-nqsohp9h 137 13 conclude conclude VBP cord-349149-nqsohp9h 137 14 that that IN cord-349149-nqsohp9h 137 15 the the DT cord-349149-nqsohp9h 137 16 IBDZ13a ibdz13a JJ cord-349149-nqsohp9h 137 17 variant variant NN cord-349149-nqsohp9h 137 18 of of IN cord-349149-nqsohp9h 137 19 IB4/91 ib4/91 NN cord-349149-nqsohp9h 137 20 is be VBZ cord-349149-nqsohp9h 137 21 involved involve VBN cord-349149-nqsohp9h 137 22 in in IN cord-349149-nqsohp9h 137 23 kidney kidney NN cord-349149-nqsohp9h 137 24 damage damage NN cord-349149-nqsohp9h 137 25 of of IN cord-349149-nqsohp9h 137 26 broiler broiler NN cord-349149-nqsohp9h 137 27 flocks flock NNS cord-349149-nqsohp9h 137 28 . . . cord-349149-nqsohp9h 138 1 When when WRB cord-349149-nqsohp9h 138 2 live live RB cord-349149-nqsohp9h 138 3 - - HYPH cord-349149-nqsohp9h 138 4 attenuated attenuate VBN cord-349149-nqsohp9h 138 5 and and CC cord-349149-nqsohp9h 138 6 inactivated inactivate VBN cord-349149-nqsohp9h 138 7 vaccines vaccine NNS cord-349149-nqsohp9h 138 8 of of IN cord-349149-nqsohp9h 138 9 IBV IBV NNP cord-349149-nqsohp9h 138 10 were be VBD cord-349149-nqsohp9h 138 11 first first RB cord-349149-nqsohp9h 138 12 introduced introduce VBN cord-349149-nqsohp9h 138 13 , , , cord-349149-nqsohp9h 138 14 they -PRON- PRP cord-349149-nqsohp9h 138 15 were be VBD cord-349149-nqsohp9h 138 16 effective effective JJ cord-349149-nqsohp9h 138 17 in in IN cord-349149-nqsohp9h 138 18 controlling control VBG cord-349149-nqsohp9h 138 19 IB IB NNP cord-349149-nqsohp9h 138 20 among among IN cord-349149-nqsohp9h 138 21 poultry poultry NN cord-349149-nqsohp9h 138 22 flocks flock NNS cord-349149-nqsohp9h 138 23 [ [ -LRB- cord-349149-nqsohp9h 138 24 33 33 CD cord-349149-nqsohp9h 138 25 ] ] -RRB- cord-349149-nqsohp9h 138 26 . . . cord-349149-nqsohp9h 139 1 However however RB cord-349149-nqsohp9h 139 2 , , , cord-349149-nqsohp9h 139 3 vaccinated vaccinated JJ cord-349149-nqsohp9h 139 4 flocks flock NNS cord-349149-nqsohp9h 139 5 in in IN cord-349149-nqsohp9h 139 6 many many JJ cord-349149-nqsohp9h 139 7 regions region NNS cord-349149-nqsohp9h 139 8 of of IN cord-349149-nqsohp9h 139 9 Algeria Algeria NNP cord-349149-nqsohp9h 139 10 have have VBP cord-349149-nqsohp9h 139 11 shown show VBN cord-349149-nqsohp9h 139 12 considerable considerable JJ cord-349149-nqsohp9h 139 13 mortality mortality NN cord-349149-nqsohp9h 139 14 and and CC cord-349149-nqsohp9h 139 15 production production NN cord-349149-nqsohp9h 139 16 losses loss NNS cord-349149-nqsohp9h 139 17 in in IN cord-349149-nqsohp9h 139 18 recent recent JJ cord-349149-nqsohp9h 139 19 years year NNS cord-349149-nqsohp9h 139 20 . . . cord-349149-nqsohp9h 140 1 The the DT cord-349149-nqsohp9h 140 2 isolation isolation NN cord-349149-nqsohp9h 140 3 of of IN cord-349149-nqsohp9h 140 4 a a DT cord-349149-nqsohp9h 140 5 new new JJ cord-349149-nqsohp9h 140 6 IBV IBV NNP cord-349149-nqsohp9h 140 7 strain strain NN cord-349149-nqsohp9h 140 8 from from IN cord-349149-nqsohp9h 140 9 the the DT cord-349149-nqsohp9h 140 10 vaccinated vaccinate VBN cord-349149-nqsohp9h 140 11 and and CC cord-349149-nqsohp9h 140 12 unvaccinated unvaccinated JJ cord-349149-nqsohp9h 140 13 flocks flock NNS cord-349149-nqsohp9h 140 14 can can MD cord-349149-nqsohp9h 140 15 be be VB cord-349149-nqsohp9h 140 16 explained explain VBN cord-349149-nqsohp9h 140 17 by by IN cord-349149-nqsohp9h 140 18 two two CD cord-349149-nqsohp9h 140 19 hypotheses hypothesis NNS cord-349149-nqsohp9h 140 20 . . . cord-349149-nqsohp9h 141 1 The the DT cord-349149-nqsohp9h 141 2 first first JJ cord-349149-nqsohp9h 141 3 hypothesis hypothesis NN cord-349149-nqsohp9h 141 4 , , , cord-349149-nqsohp9h 141 5 based base VBN cord-349149-nqsohp9h 141 6 on on IN cord-349149-nqsohp9h 141 7 the the DT cord-349149-nqsohp9h 141 8 mutations mutation NNS cord-349149-nqsohp9h 141 9 and and CC cord-349149-nqsohp9h 141 10 amino amino NN cord-349149-nqsohp9h 141 11 acid acid JJ cord-349149-nqsohp9h 141 12 alterations alteration NNS cord-349149-nqsohp9h 141 13 in in IN cord-349149-nqsohp9h 141 14 the the DT cord-349149-nqsohp9h 141 15 S1 S1 NNP cord-349149-nqsohp9h 141 16 epitope epitope NN cord-349149-nqsohp9h 141 17 , , , cord-349149-nqsohp9h 141 18 is be VBZ cord-349149-nqsohp9h 141 19 that that IN cord-349149-nqsohp9h 141 20 the the DT cord-349149-nqsohp9h 141 21 IBV IBV NNP cord-349149-nqsohp9h 141 22 strain strain NN cord-349149-nqsohp9h 141 23 evolved evolve VBN cord-349149-nqsohp9h 141 24 to to TO cord-349149-nqsohp9h 141 25 escape escape VB cord-349149-nqsohp9h 141 26 the the DT cord-349149-nqsohp9h 141 27 host host NN cord-349149-nqsohp9h 141 28 immune immune JJ cord-349149-nqsohp9h 141 29 response response NN cord-349149-nqsohp9h 141 30 elicited elicit VBN cord-349149-nqsohp9h 141 31 by by IN cord-349149-nqsohp9h 141 32 the the DT cord-349149-nqsohp9h 141 33 current current JJ cord-349149-nqsohp9h 141 34 vaccines vaccine NNS cord-349149-nqsohp9h 141 35 [ [ -LRB- cord-349149-nqsohp9h 141 36 34 34 CD cord-349149-nqsohp9h 141 37 ] ] -RRB- cord-349149-nqsohp9h 141 38 . . . cord-349149-nqsohp9h 142 1 However however RB cord-349149-nqsohp9h 142 2 , , , cord-349149-nqsohp9h 142 3 this this DT cord-349149-nqsohp9h 142 4 hypothesis hypothesis NN cord-349149-nqsohp9h 142 5 is be VBZ cord-349149-nqsohp9h 142 6 weak weak JJ cord-349149-nqsohp9h 142 7 since since IN cord-349149-nqsohp9h 142 8 amino amino NN cord-349149-nqsohp9h 142 9 acid acid NN cord-349149-nqsohp9h 142 10 alterations alteration NNS cord-349149-nqsohp9h 142 11 do do VBP cord-349149-nqsohp9h 142 12 not not RB cord-349149-nqsohp9h 142 13 always always RB cord-349149-nqsohp9h 142 14 translate translate VB cord-349149-nqsohp9h 142 15 into into IN cord-349149-nqsohp9h 142 16 differences difference NNS cord-349149-nqsohp9h 142 17 in in IN cord-349149-nqsohp9h 142 18 antigenicity antigenicity NN cord-349149-nqsohp9h 142 19 or or CC cord-349149-nqsohp9h 142 20 biological biological JJ cord-349149-nqsohp9h 142 21 function function NN cord-349149-nqsohp9h 142 22 . . . cord-349149-nqsohp9h 143 1 Furthermore furthermore RB cord-349149-nqsohp9h 143 2 , , , cord-349149-nqsohp9h 143 3 the the DT cord-349149-nqsohp9h 143 4 sequence sequence NN cord-349149-nqsohp9h 143 5 data datum NNS cord-349149-nqsohp9h 143 6 only only RB cord-349149-nqsohp9h 143 7 provide provide VBP cord-349149-nqsohp9h 143 8 information information NN cord-349149-nqsohp9h 143 9 regarding regard VBG cord-349149-nqsohp9h 143 10 the the DT cord-349149-nqsohp9h 143 11 primary primary JJ cord-349149-nqsohp9h 143 12 structure structure NN cord-349149-nqsohp9h 143 13 of of IN cord-349149-nqsohp9h 143 14 the the DT cord-349149-nqsohp9h 143 15 protein protein NN cord-349149-nqsohp9h 143 16 and and CC cord-349149-nqsohp9h 143 17 not not RB cord-349149-nqsohp9h 143 18 the the DT cord-349149-nqsohp9h 143 19 secondary secondary JJ cord-349149-nqsohp9h 143 20 and and CC cord-349149-nqsohp9h 143 21 tertiary tertiary JJ cord-349149-nqsohp9h 143 22 structures structure NNS cord-349149-nqsohp9h 143 23 which which WDT cord-349149-nqsohp9h 143 24 are be VBP cord-349149-nqsohp9h 143 25 vital vital JJ cord-349149-nqsohp9h 143 26 for for IN cord-349149-nqsohp9h 143 27 its -PRON- PRP$ cord-349149-nqsohp9h 143 28 biological biological JJ cord-349149-nqsohp9h 143 29 function function NN cord-349149-nqsohp9h 143 30 and and CC cord-349149-nqsohp9h 143 31 antigenicity antigenicity NN cord-349149-nqsohp9h 143 32 [ [ -LRB- cord-349149-nqsohp9h 143 33 23 23 CD cord-349149-nqsohp9h 143 34 ] ] -RRB- cord-349149-nqsohp9h 143 35 . . . cord-349149-nqsohp9h 144 1 The the DT cord-349149-nqsohp9h 144 2 second second JJ cord-349149-nqsohp9h 144 3 hypothesis hypothesis NN cord-349149-nqsohp9h 144 4 is be VBZ cord-349149-nqsohp9h 144 5 the the DT cord-349149-nqsohp9h 144 6 failure failure NN cord-349149-nqsohp9h 144 7 to to TO cord-349149-nqsohp9h 144 8 adopt adopt VB cord-349149-nqsohp9h 144 9 the the DT cord-349149-nqsohp9h 144 10 vaccination vaccination NN cord-349149-nqsohp9h 144 11 programs program NNS cord-349149-nqsohp9h 144 12 to to IN cord-349149-nqsohp9h 144 13 the the DT cord-349149-nqsohp9h 144 14 IBV IBV NNP cord-349149-nqsohp9h 144 15 field field NN cord-349149-nqsohp9h 144 16 strains strain NNS cord-349149-nqsohp9h 144 17 circulating circulate VBG cord-349149-nqsohp9h 144 18 in in IN cord-349149-nqsohp9h 144 19 the the DT cord-349149-nqsohp9h 144 20 farms farm NNS cord-349149-nqsohp9h 144 21 . . . cord-349149-nqsohp9h 145 1 In in IN cord-349149-nqsohp9h 145 2 our -PRON- PRP$ cord-349149-nqsohp9h 145 3 study study NN cord-349149-nqsohp9h 145 4 , , , cord-349149-nqsohp9h 145 5 only only RB cord-349149-nqsohp9h 145 6 two two CD cord-349149-nqsohp9h 145 7 flocks flock NNS cord-349149-nqsohp9h 145 8 received receive VBD cord-349149-nqsohp9h 145 9 the the DT cord-349149-nqsohp9h 145 10 combined combined JJ cord-349149-nqsohp9h 145 11 vaccine vaccine NN cord-349149-nqsohp9h 145 12 of of IN cord-349149-nqsohp9h 145 13 IB IB NNP cord-349149-nqsohp9h 145 14 Ma5 Ma5 NNP cord-349149-nqsohp9h 145 15 ( ( -LRB- cord-349149-nqsohp9h 145 16 at at IN cord-349149-nqsohp9h 145 17 day day NN cord-349149-nqsohp9h 145 18 7 7 CD cord-349149-nqsohp9h 145 19 ) ) -RRB- cord-349149-nqsohp9h 145 20 and and CC cord-349149-nqsohp9h 145 21 IB IB NNP cord-349149-nqsohp9h 145 22 4/91 4/91 CD cord-349149-nqsohp9h 145 23 ( ( -LRB- cord-349149-nqsohp9h 145 24 at at IN cord-349149-nqsohp9h 145 25 day day NN cord-349149-nqsohp9h 145 26 14 14 CD cord-349149-nqsohp9h 145 27 ) ) -RRB- cord-349149-nqsohp9h 145 28 . . . cord-349149-nqsohp9h 146 1 The the DT cord-349149-nqsohp9h 146 2 failure failure NN cord-349149-nqsohp9h 146 3 to to TO cord-349149-nqsohp9h 146 4 protect protect VB cord-349149-nqsohp9h 146 5 broilers broiler NNS cord-349149-nqsohp9h 146 6 against against IN cord-349149-nqsohp9h 146 7 nephritis nephritis NN cord-349149-nqsohp9h 146 8 could could MD cord-349149-nqsohp9h 146 9 also also RB cord-349149-nqsohp9h 146 10 be be VB cord-349149-nqsohp9h 146 11 related relate VBN cord-349149-nqsohp9h 146 12 to to IN cord-349149-nqsohp9h 146 13 the the DT cord-349149-nqsohp9h 146 14 shorter short JJR cord-349149-nqsohp9h 146 15 inter inter JJ cord-349149-nqsohp9h 146 16 - - JJ cord-349149-nqsohp9h 146 17 vaccination vaccination JJ cord-349149-nqsohp9h 146 18 interval interval NN cord-349149-nqsohp9h 146 19 of of IN cord-349149-nqsohp9h 146 20 7 7 CD cord-349149-nqsohp9h 146 21 days day NNS cord-349149-nqsohp9h 146 22 . . . cord-349149-nqsohp9h 147 1 Terregino Terregino NNP cord-349149-nqsohp9h 147 2 et et NNP cord-349149-nqsohp9h 147 3 al al NNP cord-349149-nqsohp9h 147 4 . . . cord-349149-nqsohp9h 148 1 [ [ -LRB- cord-349149-nqsohp9h 148 2 35 35 CD cord-349149-nqsohp9h 148 3 ] ] -RRB- cord-349149-nqsohp9h 148 4 demonstrated demonstrate VBN cord-349149-nqsohp9h 148 5 improved improved JJ cord-349149-nqsohp9h 148 6 protection protection NN cord-349149-nqsohp9h 148 7 against against IN cord-349149-nqsohp9h 148 8 several several JJ cord-349149-nqsohp9h 148 9 IB IB NNP cord-349149-nqsohp9h 148 10 serotypes serotype NNS cord-349149-nqsohp9h 148 11 by by IN cord-349149-nqsohp9h 148 12 vaccinating vaccinate VBG cord-349149-nqsohp9h 148 13 with with IN cord-349149-nqsohp9h 148 14 two two CD cord-349149-nqsohp9h 148 15 antigenically antigenically RB cord-349149-nqsohp9h 148 16 different different JJ cord-349149-nqsohp9h 148 17 IB IB NNP cord-349149-nqsohp9h 148 18 vaccines vaccine NNS cord-349149-nqsohp9h 148 19 given give VBN cord-349149-nqsohp9h 148 20 at at IN cord-349149-nqsohp9h 148 21 the the DT cord-349149-nqsohp9h 148 22 interval interval NN cord-349149-nqsohp9h 148 23 of of IN cord-349149-nqsohp9h 148 24 14 14 CD cord-349149-nqsohp9h 148 25 days day NNS cord-349149-nqsohp9h 148 26 . . . cord-349149-nqsohp9h 149 1 This this DT cord-349149-nqsohp9h 149 2 approach approach NN cord-349149-nqsohp9h 149 3 obviates obviate VBZ cord-349149-nqsohp9h 149 4 the the DT cord-349149-nqsohp9h 149 5 need need NN cord-349149-nqsohp9h 149 6 to to TO cord-349149-nqsohp9h 149 7 develop develop VB cord-349149-nqsohp9h 149 8 a a DT cord-349149-nqsohp9h 149 9 new new JJ cord-349149-nqsohp9h 149 10 IB IB NNP cord-349149-nqsohp9h 149 11 vaccine vaccine NN cord-349149-nqsohp9h 149 12 for for IN cord-349149-nqsohp9h 149 13 each each DT cord-349149-nqsohp9h 149 14 new new JJ cord-349149-nqsohp9h 149 15 emerging emerge VBG cord-349149-nqsohp9h 149 16 serotype serotype NN cord-349149-nqsohp9h 149 17 . . . cord-349149-nqsohp9h 150 1 One one CD cord-349149-nqsohp9h 150 2 flock flock NN cord-349149-nqsohp9h 150 3 was be VBD cord-349149-nqsohp9h 150 4 vaccinated vaccinate VBN cord-349149-nqsohp9h 150 5 with with IN cord-349149-nqsohp9h 150 6 the the DT cord-349149-nqsohp9h 150 7 Mass Mass NNP cord-349149-nqsohp9h 150 8 vaccine vaccine NN cord-349149-nqsohp9h 150 9 type type NN cord-349149-nqsohp9h 150 10 H120 h120 XX cord-349149-nqsohp9h 150 11 strain strain NN cord-349149-nqsohp9h 150 12 , , , cord-349149-nqsohp9h 150 13 which which WDT cord-349149-nqsohp9h 150 14 , , , cord-349149-nqsohp9h 150 15 however however RB cord-349149-nqsohp9h 150 16 , , , cord-349149-nqsohp9h 150 17 did do VBD cord-349149-nqsohp9h 150 18 not not RB cord-349149-nqsohp9h 150 19 provide provide VB cord-349149-nqsohp9h 150 20 sufficient sufficient JJ cord-349149-nqsohp9h 150 21 protection protection NN cord-349149-nqsohp9h 150 22 against against IN cord-349149-nqsohp9h 150 23 IBV IBV NNP cord-349149-nqsohp9h 150 24 infection infection NN cord-349149-nqsohp9h 150 25 [ [ -LRB- cord-349149-nqsohp9h 150 26 33 33 CD cord-349149-nqsohp9h 150 27 , , , cord-349149-nqsohp9h 150 28 36 36 CD cord-349149-nqsohp9h 150 29 ] ] -RRB- cord-349149-nqsohp9h 150 30 . . . cord-349149-nqsohp9h 151 1 We -PRON- PRP cord-349149-nqsohp9h 151 2 identified identify VBD cord-349149-nqsohp9h 151 3 IBDZ13a ibdz13a RB cord-349149-nqsohp9h 151 4 , , , cord-349149-nqsohp9h 151 5 a a DT cord-349149-nqsohp9h 151 6 new new JJ cord-349149-nqsohp9h 151 7 nephropathogenic nephropathogenic JJ cord-349149-nqsohp9h 151 8 variant variant NN cord-349149-nqsohp9h 151 9 of of IN cord-349149-nqsohp9h 151 10 IB IB NNP cord-349149-nqsohp9h 151 11 4/91 4/91 CD cord-349149-nqsohp9h 151 12 , , , cord-349149-nqsohp9h 151 13 for for IN cord-349149-nqsohp9h 151 14 the the DT cord-349149-nqsohp9h 151 15 1 1 CD cord-349149-nqsohp9h 151 16 st st NNP cord-349149-nqsohp9h 151 17 time time NN cord-349149-nqsohp9h 151 18 in in IN cord-349149-nqsohp9h 151 19 Algerian algerian JJ cord-349149-nqsohp9h 151 20 broiler broiler NN cord-349149-nqsohp9h 151 21 flocks flock NNS cord-349149-nqsohp9h 151 22 . . . cord-349149-nqsohp9h 152 1 Its -PRON- PRP$ cord-349149-nqsohp9h 152 2 isolation isolation NN cord-349149-nqsohp9h 152 3 from from IN cord-349149-nqsohp9h 152 4 the the DT cord-349149-nqsohp9h 152 5 vaccinated vaccinate VBN cord-349149-nqsohp9h 152 6 flocks flock NNS cord-349149-nqsohp9h 152 7 indicates indicate VBZ cord-349149-nqsohp9h 152 8 poor poor JJ cord-349149-nqsohp9h 152 9 protection protection NN cord-349149-nqsohp9h 152 10 afforded afford VBN cord-349149-nqsohp9h 152 11 by by IN cord-349149-nqsohp9h 152 12 the the DT cord-349149-nqsohp9h 152 13 vaccination vaccination NN cord-349149-nqsohp9h 152 14 programs program NNS cord-349149-nqsohp9h 152 15 to to IN cord-349149-nqsohp9h 152 16 the the DT cord-349149-nqsohp9h 152 17 broilers broiler NNS cord-349149-nqsohp9h 152 18 against against IN cord-349149-nqsohp9h 152 19 infection infection NN cord-349149-nqsohp9h 152 20 with with IN cord-349149-nqsohp9h 152 21 new new JJ cord-349149-nqsohp9h 152 22 IB IB NNP cord-349149-nqsohp9h 152 23 variants variant NNS cord-349149-nqsohp9h 152 24 . . . cord-349149-nqsohp9h 153 1 Further further JJ cord-349149-nqsohp9h 153 2 studies study NNS cord-349149-nqsohp9h 153 3 are be VBP cord-349149-nqsohp9h 153 4 needed need VBN cord-349149-nqsohp9h 153 5 to to TO cord-349149-nqsohp9h 153 6 explain explain VB cord-349149-nqsohp9h 153 7 the the DT cord-349149-nqsohp9h 153 8 failure failure NN cord-349149-nqsohp9h 153 9 of of IN cord-349149-nqsohp9h 153 10 the the DT cord-349149-nqsohp9h 153 11 current current JJ cord-349149-nqsohp9h 153 12 vaccines vaccine NNS cord-349149-nqsohp9h 153 13 against against IN cord-349149-nqsohp9h 153 14 the the DT cord-349149-nqsohp9h 153 15 new new JJ cord-349149-nqsohp9h 153 16 IB4/91 ib4/91 NN cord-349149-nqsohp9h 153 17 strain strain NN cord-349149-nqsohp9h 153 18 and and CC cord-349149-nqsohp9h 153 19 to to TO cord-349149-nqsohp9h 153 20 investigate investigate VB cord-349149-nqsohp9h 153 21 the the DT cord-349149-nqsohp9h 153 22 cross cross NN cord-349149-nqsohp9h 153 23 - - NN cord-349149-nqsohp9h 153 24 protection protection NN cord-349149-nqsohp9h 153 25 and and CC cord-349149-nqsohp9h 153 26 pathogenicity pathogenicity NN cord-349149-nqsohp9h 153 27 of of IN cord-349149-nqsohp9h 153 28 the the DT cord-349149-nqsohp9h 153 29 new new JJ cord-349149-nqsohp9h 153 30 genotype genotype NN cord-349149-nqsohp9h 153 31 . . . cord-349149-nqsohp9h 154 1 AL AL NNP cord-349149-nqsohp9h 154 2 and and CC cord-349149-nqsohp9h 154 3 MO MO NNP cord-349149-nqsohp9h 154 4 planned plan VBD cord-349149-nqsohp9h 154 5 and and CC cord-349149-nqsohp9h 154 6 designed design VBD cord-349149-nqsohp9h 154 7 the the DT cord-349149-nqsohp9h 154 8 whole whole JJ cord-349149-nqsohp9h 154 9 study study NN cord-349149-nqsohp9h 154 10 . . . cord-349149-nqsohp9h 155 1 AL AL NNP cord-349149-nqsohp9h 155 2 and and CC cord-349149-nqsohp9h 155 3 HM HM NNP cord-349149-nqsohp9h 155 4 collected collect VBD cord-349149-nqsohp9h 155 5 samples sample NNS cord-349149-nqsohp9h 155 6 , , , cord-349149-nqsohp9h 155 7 performed perform VBN cord-349149-nqsohp9h 155 8 technique technique NN cord-349149-nqsohp9h 155 9 , , , cord-349149-nqsohp9h 155 10 and and CC cord-349149-nqsohp9h 155 11 analyzed analyze VBD cord-349149-nqsohp9h 155 12 the the DT cord-349149-nqsohp9h 155 13 data datum NNS cord-349149-nqsohp9h 155 14 . . . cord-349149-nqsohp9h 156 1 KO KO NNP cord-349149-nqsohp9h 156 2 and and CC cord-349149-nqsohp9h 156 3 MO MO NNP cord-349149-nqsohp9h 156 4 supervised supervise VBD cord-349149-nqsohp9h 156 5 the the DT cord-349149-nqsohp9h 156 6 project project NN cord-349149-nqsohp9h 156 7 and and CC cord-349149-nqsohp9h 156 8 helped help VBD cord-349149-nqsohp9h 156 9 during during IN cord-349149-nqsohp9h 156 10 manuscript manuscript NN cord-349149-nqsohp9h 156 11 writing writing NN cord-349149-nqsohp9h 156 12 , , , cord-349149-nqsohp9h 156 13 crosschecking crosschecking NN cord-349149-nqsohp9h 156 14 , , , cord-349149-nqsohp9h 156 15 and and CC cord-349149-nqsohp9h 156 16 revision revision NN cord-349149-nqsohp9h 156 17 . . . cord-349149-nqsohp9h 157 1 All all DT cord-349149-nqsohp9h 157 2 authors author NNS cord-349149-nqsohp9h 157 3 read read VBP cord-349149-nqsohp9h 157 4 and and CC cord-349149-nqsohp9h 157 5 approved approve VBD cord-349149-nqsohp9h 157 6 the the DT cord-349149-nqsohp9h 157 7 final final JJ cord-349149-nqsohp9h 157 8 manuscript manuscript NN cord-349149-nqsohp9h 157 9 . . . cord-349149-nqsohp9h 158 1 Infectious infectious JJ cord-349149-nqsohp9h 158 2 bronchitis bronchitis NN cord-349149-nqsohp9h 159 1 An an DT cord-349149-nqsohp9h 159 2 apparently apparently RB cord-349149-nqsohp9h 159 3 new new JJ cord-349149-nqsohp9h 159 4 respiratory respiratory JJ cord-349149-nqsohp9h 159 5 disease disease NN cord-349149-nqsohp9h 159 6 of of IN cord-349149-nqsohp9h 159 7 baby baby NN cord-349149-nqsohp9h 159 8 chicks chick NNS cord-349149-nqsohp9h 160 1 A a DT cord-349149-nqsohp9h 160 2 new new JJ cord-349149-nqsohp9h 160 3 genotype genotype NN cord-349149-nqsohp9h 160 4 of of IN cord-349149-nqsohp9h 160 5 nephropathogenic nephropathogenic JJ cord-349149-nqsohp9h 160 6 infectious infectious JJ cord-349149-nqsohp9h 160 7 bronchitis bronchitis NN cord-349149-nqsohp9h 160 8 virus virus NN cord-349149-nqsohp9h 160 9 circulating circulate VBG cord-349149-nqsohp9h 160 10 in in IN cord-349149-nqsohp9h 160 11 vaccinated vaccinated JJ cord-349149-nqsohp9h 160 12 and and CC cord-349149-nqsohp9h 160 13 non non JJ cord-349149-nqsohp9h 160 14 - - JJ cord-349149-nqsohp9h 160 15 vaccinated vaccinated JJ cord-349149-nqsohp9h 160 16 flocks flock NNS cord-349149-nqsohp9h 160 17 in in IN cord-349149-nqsohp9h 160 18 China China NNP cord-349149-nqsohp9h 160 19 Etiology Etiology NNP cord-349149-nqsohp9h 160 20 of of IN cord-349149-nqsohp9h 160 21 an an DT cord-349149-nqsohp9h 160 22 infectious infectious JJ cord-349149-nqsohp9h 160 23 nephritis nephritis NN cord-349149-nqsohp9h 160 24 - - HYPH cord-349149-nqsohp9h 160 25 nephrosis nephrosis NN cord-349149-nqsohp9h 160 26 syndrome syndrome NN cord-349149-nqsohp9h 160 27 of of IN cord-349149-nqsohp9h 160 28 chickens chicken NNS cord-349149-nqsohp9h 160 29 Avian avian JJ cord-349149-nqsohp9h 160 30 infectious infectious JJ cord-349149-nqsohp9h 160 31 bronchitis bronchitis NN cord-349149-nqsohp9h 160 32 : : : cord-349149-nqsohp9h 160 33 Characterization characterization NN cord-349149-nqsohp9h 160 34 of of IN cord-349149-nqsohp9h 160 35 new new JJ cord-349149-nqsohp9h 160 36 isolates isolate NNS cord-349149-nqsohp9h 160 37 from from IN cord-349149-nqsohp9h 160 38 Italy Italy NNP cord-349149-nqsohp9h 160 39 Isolation Isolation NNP cord-349149-nqsohp9h 160 40 and and CC cord-349149-nqsohp9h 160 41 identification identification NN cord-349149-nqsohp9h 160 42 of of IN cord-349149-nqsohp9h 160 43 four four CD cord-349149-nqsohp9h 160 44 isolates isolate NNS cord-349149-nqsohp9h 160 45 of of IN cord-349149-nqsohp9h 160 46 infectious infectious JJ cord-349149-nqsohp9h 160 47 bronchitis bronchitis NN cord-349149-nqsohp9h 160 48 strains strain NNS cord-349149-nqsohp9h 160 49 in in IN cord-349149-nqsohp9h 160 50 China China NNP cord-349149-nqsohp9h 160 51 and and CC cord-349149-nqsohp9h 160 52 analysis analysis NN cord-349149-nqsohp9h 160 53 of of IN cord-349149-nqsohp9h 160 54 their -PRON- PRP$ cord-349149-nqsohp9h 160 55 SI SI NNP cord-349149-nqsohp9h 160 56 protein protein NN cord-349149-nqsohp9h 160 57 gene gene NN cord-349149-nqsohp9h 160 58 Isolation isolation NN cord-349149-nqsohp9h 160 59 of of IN cord-349149-nqsohp9h 160 60 4/91 4/91 CD cord-349149-nqsohp9h 160 61 type type NN cord-349149-nqsohp9h 160 62 of of IN cord-349149-nqsohp9h 160 63 infectious infectious JJ cord-349149-nqsohp9h 160 64 bronchitis bronchitis NN cord-349149-nqsohp9h 160 65 virus virus NN cord-349149-nqsohp9h 160 66 as as IN cord-349149-nqsohp9h 160 67 a a DT cord-349149-nqsohp9h 160 68 new new JJ cord-349149-nqsohp9h 160 69 variant variant NN cord-349149-nqsohp9h 160 70 in in IN cord-349149-nqsohp9h 160 71 Japan Japan NNP cord-349149-nqsohp9h 160 72 and and CC cord-349149-nqsohp9h 160 73 efficacy efficacy NN cord-349149-nqsohp9h 160 74 of of IN cord-349149-nqsohp9h 160 75 vaccination vaccination NN cord-349149-nqsohp9h 160 76 against against IN cord-349149-nqsohp9h 160 77 4/91 4/91 CD cord-349149-nqsohp9h 160 78 type type NN cord-349149-nqsohp9h 160 79 field field NN cord-349149-nqsohp9h 160 80 isolate isolate VBP cord-349149-nqsohp9h 160 81 An an DT cord-349149-nqsohp9h 160 82 overview overview NN cord-349149-nqsohp9h 160 83 of of IN cord-349149-nqsohp9h 160 84 infectious infectious JJ cord-349149-nqsohp9h 160 85 bronchitis bronchitis NN cord-349149-nqsohp9h 160 86 virus virus NN cord-349149-nqsohp9h 160 87 in in IN cord-349149-nqsohp9h 160 88 chickens chicken NNS cord-349149-nqsohp9h 160 89 Phylogenetic Phylogenetic NNP cord-349149-nqsohp9h 160 90 analysis analysis NN cord-349149-nqsohp9h 160 91 of of IN cord-349149-nqsohp9h 160 92 avian avian JJ cord-349149-nqsohp9h 160 93 infectious infectious JJ cord-349149-nqsohp9h 160 94 bronchitis bronchitis NN cord-349149-nqsohp9h 160 95 virus virus NN cord-349149-nqsohp9h 160 96 S1 s1 NN cord-349149-nqsohp9h 160 97 glycoprotein glycoprotein NN cord-349149-nqsohp9h 160 98 regions region NNS cord-349149-nqsohp9h 160 99 reveals reveal VBZ cord-349149-nqsohp9h 160 100 emergence emergence NN cord-349149-nqsohp9h 160 101 of of IN cord-349149-nqsohp9h 160 102 a a DT cord-349149-nqsohp9h 160 103 new new JJ cord-349149-nqsohp9h 160 104 genotype genotype NN cord-349149-nqsohp9h 160 105 in in IN cord-349149-nqsohp9h 160 106 Moroccan moroccan JJ cord-349149-nqsohp9h 160 107 broiler broiler NN cord-349149-nqsohp9h 160 108 chicken chicken NN cord-349149-nqsohp9h 160 109 flocks flock NNS cord-349149-nqsohp9h 160 110 S1 S1 NNP cord-349149-nqsohp9h 160 111 gene gene NN cord-349149-nqsohp9h 160 112 sequence sequence NN cord-349149-nqsohp9h 160 113 analysis analysis NN cord-349149-nqsohp9h 160 114 of of IN cord-349149-nqsohp9h 160 115 a a DT cord-349149-nqsohp9h 160 116 nephropathogenic nephropathogenic JJ cord-349149-nqsohp9h 160 117 strain strain NN cord-349149-nqsohp9h 160 118 of of IN cord-349149-nqsohp9h 160 119 avian avian JJ cord-349149-nqsohp9h 160 120 infectious infectious JJ cord-349149-nqsohp9h 160 121 bronchitis bronchitis NN cord-349149-nqsohp9h 160 122 virus virus NN cord-349149-nqsohp9h 160 123 in in IN cord-349149-nqsohp9h 160 124 Egypt Egypt NNP cord-349149-nqsohp9h 160 125 Variation Variation NNP cord-349149-nqsohp9h 160 126 in in IN cord-349149-nqsohp9h 160 127 the the DT cord-349149-nqsohp9h 160 128 spike spike NN cord-349149-nqsohp9h 160 129 protein protein NN cord-349149-nqsohp9h 160 130 of of IN cord-349149-nqsohp9h 160 131 the the DT cord-349149-nqsohp9h 160 132 793 793 CD cord-349149-nqsohp9h 160 133 / / SYM cord-349149-nqsohp9h 160 134 B b NN cord-349149-nqsohp9h 160 135 type type NN cord-349149-nqsohp9h 160 136 of of IN cord-349149-nqsohp9h 160 137 infectious infectious JJ cord-349149-nqsohp9h 160 138 bronchitis bronchitis NN cord-349149-nqsohp9h 160 139 virus virus NN cord-349149-nqsohp9h 160 140 , , , cord-349149-nqsohp9h 160 141 in in IN cord-349149-nqsohp9h 160 142 the the DT cord-349149-nqsohp9h 160 143 field field NN cord-349149-nqsohp9h 160 144 and and CC cord-349149-nqsohp9h 160 145 during during IN cord-349149-nqsohp9h 160 146 alternate alternate JJ cord-349149-nqsohp9h 160 147 passage passage NN cord-349149-nqsohp9h 160 148 in in IN cord-349149-nqsohp9h 160 149 chickens chicken NNS cord-349149-nqsohp9h 160 150 and and CC cord-349149-nqsohp9h 160 151 embryonated embryonate VBN cord-349149-nqsohp9h 160 152 eggs egg NNS cord-349149-nqsohp9h 160 153 Isolation isolation NN cord-349149-nqsohp9h 160 154 and and CC cord-349149-nqsohp9h 160 155 genetic genetic JJ cord-349149-nqsohp9h 160 156 analysis analysis NN cord-349149-nqsohp9h 160 157 revealed reveal VBD cord-349149-nqsohp9h 160 158 no no DT cord-349149-nqsohp9h 160 159 predominant predominant JJ cord-349149-nqsohp9h 160 160 new new JJ cord-349149-nqsohp9h 160 161 strains strain NNS cord-349149-nqsohp9h 160 162 of of IN cord-349149-nqsohp9h 160 163 avian avian JJ cord-349149-nqsohp9h 160 164 infectious infectious JJ cord-349149-nqsohp9h 160 165 bronchitis bronchitis NN cord-349149-nqsohp9h 160 166 virus virus NN cord-349149-nqsohp9h 160 167 circulating circulate VBG cord-349149-nqsohp9h 160 168 in in IN cord-349149-nqsohp9h 160 169 South South NNP cord-349149-nqsohp9h 160 170 China China NNP cord-349149-nqsohp9h 160 171 during during IN cord-349149-nqsohp9h 160 172 A a DT cord-349149-nqsohp9h 160 173 reverse reverse JJ cord-349149-nqsohp9h 160 174 transcriptase transcriptase NN cord-349149-nqsohp9h 160 175 polymerase polymerase NN cord-349149-nqsohp9h 160 176 chain chain NN cord-349149-nqsohp9h 160 177 reaction reaction NN cord-349149-nqsohp9h 160 178 survey survey NN cord-349149-nqsohp9h 160 179 of of IN cord-349149-nqsohp9h 160 180 infectious infectious JJ cord-349149-nqsohp9h 160 181 bronchitis bronchitis NN cord-349149-nqsohp9h 160 182 virus virus NN cord-349149-nqsohp9h 160 183 genotypes genotype NNS cord-349149-nqsohp9h 160 184 in in IN cord-349149-nqsohp9h 160 185 Western Western NNP cord-349149-nqsohp9h 160 186 Europe Europe NNP cord-349149-nqsohp9h 160 187 from from IN cord-349149-nqsohp9h 160 188 Molecular Molecular NNP cord-349149-nqsohp9h 160 189 epizootiology epizootiology NN cord-349149-nqsohp9h 160 190 of of IN cord-349149-nqsohp9h 160 191 avian avian JJ cord-349149-nqsohp9h 160 192 infectious infectious JJ cord-349149-nqsohp9h 160 193 bronchitis bronchitis NN cord-349149-nqsohp9h 160 194 virus virus NN cord-349149-nqsohp9h 160 195 in in IN cord-349149-nqsohp9h 160 196 Russia Russia NNP cord-349149-nqsohp9h 161 1 Coronavirus Coronavirus NNP cord-349149-nqsohp9h 161 2 avian avian JJ cord-349149-nqsohp9h 161 3 infectious infectious JJ cord-349149-nqsohp9h 161 4 bronchitis bronchitis NN cord-349149-nqsohp9h 161 5 virus virus NN cord-349149-nqsohp9h 161 6 Evidence evidence NN cord-349149-nqsohp9h 161 7 of of IN cord-349149-nqsohp9h 161 8 natural natural JJ cord-349149-nqsohp9h 161 9 recombination recombination NN cord-349149-nqsohp9h 161 10 within within IN cord-349149-nqsohp9h 161 11 the the DT cord-349149-nqsohp9h 161 12 S1 S1 NNP cord-349149-nqsohp9h 161 13 gene gene NN cord-349149-nqsohp9h 161 14 of of IN cord-349149-nqsohp9h 161 15 infectious infectious JJ cord-349149-nqsohp9h 161 16 bronchitis bronchitis NN cord-349149-nqsohp9h 161 17 virus virus NN cord-349149-nqsohp9h 161 18 Gene gene NN cord-349149-nqsohp9h 161 19 characteristics characteristic NNS cord-349149-nqsohp9h 161 20 and and CC cord-349149-nqsohp9h 161 21 efficacy efficacy NN cord-349149-nqsohp9h 161 22 of of IN cord-349149-nqsohp9h 161 23 vaccination vaccination NN cord-349149-nqsohp9h 161 24 against against IN cord-349149-nqsohp9h 161 25 infectious infectious JJ cord-349149-nqsohp9h 161 26 bronchitis bronchitis NN cord-349149-nqsohp9h 161 27 virus virus NN cord-349149-nqsohp9h 161 28 field field NN cord-349149-nqsohp9h 161 29 isolates isolate VBZ cord-349149-nqsohp9h 161 30 from from IN cord-349149-nqsohp9h 161 31 the the DT cord-349149-nqsohp9h 161 32 United United NNP cord-349149-nqsohp9h 161 33 States States NNP cord-349149-nqsohp9h 161 34 and and CC cord-349149-nqsohp9h 161 35 Israel Israel NNP cord-349149-nqsohp9h 161 36 Characterization Characterization NNP cord-349149-nqsohp9h 161 37 and and CC cord-349149-nqsohp9h 161 38 analysis analysis NN cord-349149-nqsohp9h 161 39 of of IN cord-349149-nqsohp9h 161 40 an an DT cord-349149-nqsohp9h 161 41 infectious infectious JJ cord-349149-nqsohp9h 161 42 bronchitis bronchitis NN cord-349149-nqsohp9h 161 43 virus virus NN cord-349149-nqsohp9h 161 44 strain strain NN cord-349149-nqsohp9h 161 45 isolated isolate VBN cord-349149-nqsohp9h 161 46 from from IN cord-349149-nqsohp9h 161 47 southern southern JJ cord-349149-nqsohp9h 161 48 China China NNP cord-349149-nqsohp9h 161 49 in in IN cord-349149-nqsohp9h 161 50 2013 2013 CD cord-349149-nqsohp9h 161 51 Genetic genetic JJ cord-349149-nqsohp9h 161 52 grouping grouping NN cord-349149-nqsohp9h 161 53 of of IN cord-349149-nqsohp9h 161 54 nephropathogenic nephropathogenic JJ cord-349149-nqsohp9h 161 55 avian avian JJ cord-349149-nqsohp9h 161 56 infectious infectious JJ cord-349149-nqsohp9h 161 57 bronchitis bronchitis NN cord-349149-nqsohp9h 161 58 virus virus NN cord-349149-nqsohp9h 161 59 isolated isolate VBN cord-349149-nqsohp9h 161 60 in in IN cord-349149-nqsohp9h 161 61 Morocco Morocco NNP cord-349149-nqsohp9h 161 62 Virulent Virulent NNP cord-349149-nqsohp9h 161 63 avian avian JJ cord-349149-nqsohp9h 161 64 infectious infectious JJ cord-349149-nqsohp9h 161 65 bronchitis bronchitis NN cord-349149-nqsohp9h 161 66 virus virus NN cord-349149-nqsohp9h 161 67 , , , cord-349149-nqsohp9h 161 68 people people NNS cord-349149-nqsohp9h 161 69 's 's POS cord-349149-nqsohp9h 161 70 republic republic NN cord-349149-nqsohp9h 161 71 of of IN cord-349149-nqsohp9h 161 72 China China NNP cord-349149-nqsohp9h 161 73 ) ) -RRB- cord-349149-nqsohp9h 161 74 Isolation isolation NN cord-349149-nqsohp9h 161 75 , , , cord-349149-nqsohp9h 161 76 identification identification NN cord-349149-nqsohp9h 161 77 and and CC cord-349149-nqsohp9h 161 78 molecular molecular JJ cord-349149-nqsohp9h 161 79 characterization characterization NN cord-349149-nqsohp9h 161 80 of of IN cord-349149-nqsohp9h 161 81 IBV IBV NNP cord-349149-nqsohp9h 161 82 variant variant NN cord-349149-nqsohp9h 161 83 from from IN cord-349149-nqsohp9h 161 84 outbreak outbreak NN cord-349149-nqsohp9h 161 85 of of IN cord-349149-nqsohp9h 161 86 visceral visceral JJ cord-349149-nqsohp9h 161 87 gout gout NN cord-349149-nqsohp9h 161 88 in in IN cord-349149-nqsohp9h 161 89 commercial commercial JJ cord-349149-nqsohp9h 161 90 Descriptive descriptive JJ cord-349149-nqsohp9h 161 91 study study NN cord-349149-nqsohp9h 161 92 of of IN cord-349149-nqsohp9h 161 93 an an DT cord-349149-nqsohp9h 161 94 outbreak outbreak NN cord-349149-nqsohp9h 161 95 of of IN cord-349149-nqsohp9h 161 96 avian avian JJ cord-349149-nqsohp9h 161 97 urolithiasis urolithiasis NN cord-349149-nqsohp9h 161 98 in in IN cord-349149-nqsohp9h 161 99 a a DT cord-349149-nqsohp9h 161 100 large large JJ cord-349149-nqsohp9h 161 101 commercial commercial JJ cord-349149-nqsohp9h 161 102 egg egg NN cord-349149-nqsohp9h 161 103 complex complex NN cord-349149-nqsohp9h 161 104 in in IN cord-349149-nqsohp9h 161 105 Algeria Algeria NNP cord-349149-nqsohp9h 161 106 Detection Detection NNP cord-349149-nqsohp9h 161 107 of of IN cord-349149-nqsohp9h 161 108 infectious infectious JJ cord-349149-nqsohp9h 161 109 bronchitis bronchitis NN cord-349149-nqsohp9h 161 110 virus virus NN cord-349149-nqsohp9h 161 111 Serological serological JJ cord-349149-nqsohp9h 161 112 comparison comparison NN cord-349149-nqsohp9h 161 113 and and CC cord-349149-nqsohp9h 161 114 antigenic antigenic JJ cord-349149-nqsohp9h 161 115 relationships relationship NNS cord-349149-nqsohp9h 161 116 of of IN cord-349149-nqsohp9h 161 117 seven seven CD cord-349149-nqsohp9h 161 118 serotypes serotype NNS cord-349149-nqsohp9h 161 119 of of IN cord-349149-nqsohp9h 161 120 infectious infectious JJ cord-349149-nqsohp9h 161 121 bronchitis bronchitis NN cord-349149-nqsohp9h 161 122 virus virus NN cord-349149-nqsohp9h 161 123 using use VBG cord-349149-nqsohp9h 161 124 the the DT cord-349149-nqsohp9h 161 125 haemagglutination haemagglutination NN cord-349149-nqsohp9h 161 126 - - HYPH cord-349149-nqsohp9h 161 127 inhibition inhibition NN cord-349149-nqsohp9h 161 128 test test NN cord-349149-nqsohp9h 161 129 Application Application NNP cord-349149-nqsohp9h 161 130 of of IN cord-349149-nqsohp9h 161 131 the the DT cord-349149-nqsohp9h 161 132 haemagglutination haemagglutination NN cord-349149-nqsohp9h 161 133 inhibition inhibition NN cord-349149-nqsohp9h 161 134 test test NN cord-349149-nqsohp9h 161 135 to to IN cord-349149-nqsohp9h 161 136 typing type VBG cord-349149-nqsohp9h 161 137 of of IN cord-349149-nqsohp9h 162 1 infectious infectious JJ cord-349149-nqsohp9h 162 2 bronchitis bronchitis NN cord-349149-nqsohp9h 162 3 virus virus NN cord-349149-nqsohp9h 162 4 Comparison comparison NN cord-349149-nqsohp9h 162 5 of of IN cord-349149-nqsohp9h 162 6 the the DT cord-349149-nqsohp9h 162 7 haemagglutination haemagglutination NN cord-349149-nqsohp9h 162 8 inhibition inhibition NN cord-349149-nqsohp9h 162 9 test test NN cord-349149-nqsohp9h 162 10 and and CC cord-349149-nqsohp9h 162 11 the the DT cord-349149-nqsohp9h 162 12 serum serum NN cord-349149-nqsohp9h 162 13 neutralization neutralization NN cord-349149-nqsohp9h 162 14 test test NN cord-349149-nqsohp9h 162 15 in in IN cord-349149-nqsohp9h 162 16 tracheal tracheal JJ cord-349149-nqsohp9h 162 17 organ organ NN cord-349149-nqsohp9h 162 18 cultures culture NNS cord-349149-nqsohp9h 162 19 for for IN cord-349149-nqsohp9h 162 20 typing type VBG cord-349149-nqsohp9h 162 21 infectious infectious JJ cord-349149-nqsohp9h 162 22 bronchitis bronchitis NN cord-349149-nqsohp9h 162 23 virus virus NN cord-349149-nqsohp9h 162 24 strains strain VBZ cord-349149-nqsohp9h 162 25 Manual Manual NNP cord-349149-nqsohp9h 162 26 of of IN cord-349149-nqsohp9h 162 27 Diagnostic Diagnostic NNP cord-349149-nqsohp9h 162 28 Tests Tests NNPS cord-349149-nqsohp9h 162 29 and and CC cord-349149-nqsohp9h 162 30 Vaccines Vaccines NNPS cord-349149-nqsohp9h 162 31 for for IN cord-349149-nqsohp9h 162 32 Terrestrial Terrestrial NNP cord-349149-nqsohp9h 162 33 Animals Animals NNPS cord-349149-nqsohp9h 162 34 . . . cord-349149-nqsohp9h 163 1 World World NNP cord-349149-nqsohp9h 163 2 Organisation Organisation NNP cord-349149-nqsohp9h 163 3 for for IN cord-349149-nqsohp9h 163 4 Animal Animal NNP cord-349149-nqsohp9h 163 5 Health Health NNP cord-349149-nqsohp9h 163 6 Infectious infectious JJ cord-349149-nqsohp9h 163 7 bronchitis bronchitis NN cord-349149-nqsohp9h 163 8 virus virus NN cord-349149-nqsohp9h 163 9 variants variant NNS cord-349149-nqsohp9h 163 10 history history NN cord-349149-nqsohp9h 163 11 , , , cord-349149-nqsohp9h 163 12 current current JJ cord-349149-nqsohp9h 163 13 situation situation NN cord-349149-nqsohp9h 163 14 and and CC cord-349149-nqsohp9h 163 15 control control NN cord-349149-nqsohp9h 163 16 measures measure NNS cord-349149-nqsohp9h 163 17 Genetic genetic JJ cord-349149-nqsohp9h 163 18 diversity diversity NN cord-349149-nqsohp9h 163 19 of of IN cord-349149-nqsohp9h 163 20 avian avian JJ cord-349149-nqsohp9h 163 21 infectious infectious JJ cord-349149-nqsohp9h 163 22 bronchitis bronchitis NN cord-349149-nqsohp9h 163 23 coronavirus coronavirus NN cord-349149-nqsohp9h 163 24 strains strain NNS cord-349149-nqsohp9h 163 25 isolated isolate VBN cord-349149-nqsohp9h 163 26 in in IN cord-349149-nqsohp9h 163 27 China China NNP cord-349149-nqsohp9h 163 28 between between IN cord-349149-nqsohp9h 163 29 Genotypes Genotypes NNP cord-349149-nqsohp9h 163 30 of of IN cord-349149-nqsohp9h 163 31 infectious infectious JJ cord-349149-nqsohp9h 163 32 bronchitis bronchitis NN cord-349149-nqsohp9h 163 33 viruses virus NNS cord-349149-nqsohp9h 163 34 circulating circulate VBG cord-349149-nqsohp9h 163 35 in in IN cord-349149-nqsohp9h 163 36 the the DT cord-349149-nqsohp9h 163 37 Middle Middle NNP cord-349149-nqsohp9h 163 38 East East NNP cord-349149-nqsohp9h 163 39 between between IN cord-349149-nqsohp9h 163 40 Phylogeny Phylogeny NNP cord-349149-nqsohp9h 163 41 of of IN cord-349149-nqsohp9h 163 42 antigenic antigenic JJ cord-349149-nqsohp9h 163 43 variants variant NNS cord-349149-nqsohp9h 163 44 of of IN cord-349149-nqsohp9h 163 45 avian avian JJ cord-349149-nqsohp9h 163 46 coronavirus coronavirus NN cord-349149-nqsohp9h 164 1 IBV IBV NNP cord-349149-nqsohp9h 164 2 A a DT cord-349149-nqsohp9h 164 3 standard standard JJ cord-349149-nqsohp9h 164 4 technique technique NN cord-349149-nqsohp9h 164 5 for for IN cord-349149-nqsohp9h 164 6 haemagglutination haemagglutination NN cord-349149-nqsohp9h 164 7 inhibition inhibition NN cord-349149-nqsohp9h 164 8 tests test NNS cord-349149-nqsohp9h 164 9 for for IN cord-349149-nqsohp9h 164 10 antibodies antibody NNS cord-349149-nqsohp9h 164 11 to to IN cord-349149-nqsohp9h 164 12 avian avian JJ cord-349149-nqsohp9h 164 13 infectious infectious JJ cord-349149-nqsohp9h 164 14 bronchitis bronchitis NN cord-349149-nqsohp9h 164 15 virus virus NN cord-349149-nqsohp9h 164 16 Protection Protection NNP cord-349149-nqsohp9h 164 17 of of IN cord-349149-nqsohp9h 164 18 chickens chicken NNS cord-349149-nqsohp9h 164 19 against against IN cord-349149-nqsohp9h 164 20 renal renal JJ cord-349149-nqsohp9h 164 21 damage damage NN cord-349149-nqsohp9h 164 22 caused cause VBN cord-349149-nqsohp9h 164 23 by by IN cord-349149-nqsohp9h 164 24 a a DT cord-349149-nqsohp9h 164 25 nephropathogenic nephropathogenic JJ cord-349149-nqsohp9h 164 26 infectious infectious JJ cord-349149-nqsohp9h 164 27 bronchitis bronchitis NN cord-349149-nqsohp9h 164 28 virus virus NN cord-349149-nqsohp9h 164 29 Review Review NNP cord-349149-nqsohp9h 164 30 of of IN cord-349149-nqsohp9h 164 31 infectious infectious JJ cord-349149-nqsohp9h 164 32 bronchitis bronchitis NN cord-349149-nqsohp9h 164 33 virus virus NN cord-349149-nqsohp9h 164 34 around around IN cord-349149-nqsohp9h 164 35 the the DT cord-349149-nqsohp9h 164 36 world world NN cord-349149-nqsohp9h 164 37 Pathogenicity Pathogenicity NNP cord-349149-nqsohp9h 164 38 of of IN cord-349149-nqsohp9h 164 39 a a DT cord-349149-nqsohp9h 164 40 QX QX NNP cord-349149-nqsohp9h 164 41 strain strain NN cord-349149-nqsohp9h 164 42 of of IN cord-349149-nqsohp9h 164 43 infectious infectious JJ cord-349149-nqsohp9h 164 44 bronchitis bronchitis NN cord-349149-nqsohp9h 164 45 virus virus NN cord-349149-nqsohp9h 164 46 in in IN cord-349149-nqsohp9h 164 47 specific specific JJ cord-349149-nqsohp9h 164 48 pathogen pathogen NN cord-349149-nqsohp9h 164 49 - - HYPH cord-349149-nqsohp9h 164 50 free free JJ cord-349149-nqsohp9h 164 51 and and CC cord-349149-nqsohp9h 164 52 commercial commercial JJ cord-349149-nqsohp9h 164 53 broiler broiler NN cord-349149-nqsohp9h 164 54 chickens chicken NNS cord-349149-nqsohp9h 164 55 , , , cord-349149-nqsohp9h 164 56 and and CC cord-349149-nqsohp9h 164 57 evaluation evaluation NN cord-349149-nqsohp9h 164 58 of of IN cord-349149-nqsohp9h 164 59 protection protection NN cord-349149-nqsohp9h 164 60 induced induce VBN cord-349149-nqsohp9h 164 61 by by IN cord-349149-nqsohp9h 164 62 a a DT cord-349149-nqsohp9h 164 63 vaccination vaccination NN cord-349149-nqsohp9h 164 64 programme programme NN cord-349149-nqsohp9h 164 65 based base VBN cord-349149-nqsohp9h 164 66 on on IN cord-349149-nqsohp9h 164 67 the the DT cord-349149-nqsohp9h 164 68 Ma5 ma5 NN cord-349149-nqsohp9h 164 69 and and CC cord-349149-nqsohp9h 164 70 4/91 4/91 CD cord-349149-nqsohp9h 164 71 serotypes serotype NNS cord-349149-nqsohp9h 164 72 Efficacy Efficacy NNP cord-349149-nqsohp9h 164 73 of of IN cord-349149-nqsohp9h 164 74 different different JJ cord-349149-nqsohp9h 164 75 vaccination vaccination NN cord-349149-nqsohp9h 164 76 programs program NNS cord-349149-nqsohp9h 164 77 against against IN cord-349149-nqsohp9h 164 78 Thai Thai NNP cord-349149-nqsohp9h 164 79 QX QX NNP cord-349149-nqsohp9h 164 80 - - HYPH cord-349149-nqsohp9h 164 81 like like JJ cord-349149-nqsohp9h 164 82 infectious infectious JJ cord-349149-nqsohp9h 164 83 bronchitis bronchitis NN cord-349149-nqsohp9h 164 84 virus virus NN cord-349149-nqsohp9h 165 1 The the DT cord-349149-nqsohp9h 165 2 authors author NNS cord-349149-nqsohp9h 165 3 gratefully gratefully RB cord-349149-nqsohp9h 165 4 acknowledge acknowledge VBP cord-349149-nqsohp9h 165 5 the the DT cord-349149-nqsohp9h 165 6 MSD MSD NNP cord-349149-nqsohp9h 165 7 International International NNP cord-349149-nqsohp9h 165 8 Laboratory Laboratory NNP cord-349149-nqsohp9h 165 9 , , , cord-349149-nqsohp9h 165 10 AMMAN AMMAN NNP cord-349149-nqsohp9h 165 11 , , , cord-349149-nqsohp9h 165 12 Jordan Jordan NNP cord-349149-nqsohp9h 165 13 , , , cord-349149-nqsohp9h 165 14 and and CC cord-349149-nqsohp9h 165 15 MSD MSD NNP cord-349149-nqsohp9h 165 16 , , , cord-349149-nqsohp9h 165 17 Algeria Algeria NNP cord-349149-nqsohp9h 165 18 , , , cord-349149-nqsohp9h 165 19 for for IN cord-349149-nqsohp9h 165 20 providing provide VBG cord-349149-nqsohp9h 165 21 the the DT cord-349149-nqsohp9h 165 22 facilitation facilitation NN cord-349149-nqsohp9h 165 23 and and CC cord-349149-nqsohp9h 165 24 equipment equipment NN cord-349149-nqsohp9h 165 25 throughout throughout IN cord-349149-nqsohp9h 165 26 the the DT cord-349149-nqsohp9h 165 27 study study NN cord-349149-nqsohp9h 165 28 . . . cord-349149-nqsohp9h 166 1 The the DT cord-349149-nqsohp9h 166 2 authors author NNS cord-349149-nqsohp9h 166 3 are be VBP cord-349149-nqsohp9h 166 4 also also RB cord-349149-nqsohp9h 166 5 thankful thankful JJ cord-349149-nqsohp9h 166 6 to to IN cord-349149-nqsohp9h 166 7 Entisar Entisar NNP cord-349149-nqsohp9h 166 8 and and CC cord-349149-nqsohp9h 166 9 Husam Husam NNP cord-349149-nqsohp9h 166 10 Bakri Bakri NNP cord-349149-nqsohp9h 166 11 for for IN cord-349149-nqsohp9h 166 12 technical technical JJ cord-349149-nqsohp9h 166 13 assistance assistance NN cord-349149-nqsohp9h 166 14 and and CC cord-349149-nqsohp9h 166 15 help help NN cord-349149-nqsohp9h 166 16 with with IN cord-349149-nqsohp9h 166 17 the the DT cord-349149-nqsohp9h 166 18 HI HI NNP cord-349149-nqsohp9h 166 19 test test NN cord-349149-nqsohp9h 166 20 analysis analysis NN cord-349149-nqsohp9h 166 21 . . . cord-349149-nqsohp9h 167 1 The the DT cord-349149-nqsohp9h 167 2 authors author NNS cord-349149-nqsohp9h 167 3 had have VBD cord-349149-nqsohp9h 167 4 used use VBN cord-349149-nqsohp9h 167 5 the the DT cord-349149-nqsohp9h 167 6 facility facility NN cord-349149-nqsohp9h 167 7 of of IN cord-349149-nqsohp9h 167 8 MSD MSD NNP cord-349149-nqsohp9h 167 9 International International NNP cord-349149-nqsohp9h 167 10 Laboratory Laboratory NNP cord-349149-nqsohp9h 167 11 , , , cord-349149-nqsohp9h 167 12 Jordan Jordan NNP cord-349149-nqsohp9h 167 13 but but CC cord-349149-nqsohp9h 167 14 no no DT cord-349149-nqsohp9h 167 15 person person NN cord-349149-nqsohp9h 167 16 from from IN cord-349149-nqsohp9h 167 17 MSD MSD NNP cord-349149-nqsohp9h 167 18 was be VBD cord-349149-nqsohp9h 167 19 involved involve VBN cord-349149-nqsohp9h 167 20 at at IN cord-349149-nqsohp9h 167 21 any any DT cord-349149-nqsohp9h 167 22 stage stage NN cord-349149-nqsohp9h 167 23 of of IN cord-349149-nqsohp9h 167 24 the the DT cord-349149-nqsohp9h 167 25 experiment experiment NN cord-349149-nqsohp9h 167 26 or or CC cord-349149-nqsohp9h 167 27 publication publication NN cord-349149-nqsohp9h 167 28 . . . cord-349149-nqsohp9h 168 1 Also also RB cord-349149-nqsohp9h 168 2 , , , cord-349149-nqsohp9h 168 3 authors author NNS cord-349149-nqsohp9h 168 4 have have VBP cord-349149-nqsohp9h 168 5 not not RB cord-349149-nqsohp9h 168 6 received receive VBN cord-349149-nqsohp9h 168 7 any any DT cord-349149-nqsohp9h 168 8 fund fund NN cord-349149-nqsohp9h 168 9 from from IN cord-349149-nqsohp9h 168 10 MSD MSD NNP cord-349149-nqsohp9h 168 11 Algeria Algeria NNP cord-349149-nqsohp9h 168 12 . . .