id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-310861-9kb0b6rq	Koo, Bonhan	An isothermal, label-free, and rapid one-step RNA amplification/detection assay for diagnosis of respiratory viral infections	2017-04-15		.txt	text/plain	5584	295	58	In this study, we report an isothermal and rapid one-step RNA amplification/detection (iROAD) assay to simultaneously amplify and detect the viral RNA in a label-free and real-time manner. Furthermore, we demonstrated the clinical utility of the iROAD assay by detecting respiratory viral RNAs extracted from the 63 nasopharyngeal samples with either IFN-A/B or HCoV-OC43/229E or RSV-A/B. Subsequently, the iROAD assay detected the respiratory viral targets from the clinical sample by measuring the resonance wavelength shift within 15-20 min (Fig. 1) . In case of the iROAD assay with label-free and real-time capabilities, the wavelength shift was sequentially increased based on the concentrations (2.5×10 1 to 10 5 copies/reaction) of IFN-B within 20 min, as compared to the negative control. In the case of the real-time RT-PCR assay, the fluorescent SYBR Green signal was observed in RNA samples diluted up to 2.5×10 2 copies/reaction and showed good linearity (R 2 =0.9795) for different concentrations of the target (Fig. 3C ).	./cache/cord-310861-9kb0b6rq.txt	./txt/cord-310861-9kb0b6rq.txt