id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-326762-t89jtjmi	Chen, Weiye	Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β	2011-06-30		.txt	text/plain	4042	185	50	title: Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β Abstract A CHO cell clone (CHO-PoIFN-β) with stable porcine IFN-β expression under control of CMV promoter was selected under G418 pressure. The purpose of this study was to establish highly efficient and stable expression of recombinant porcine IFN-b in CHO-K1 cell line, and further characterize the biological activity of the product. In order to study the antiviral capacity of recombinant PoIFN-b against porcine viruses in vitro, PK15 cells were treated with 10-fold serially diluted PoIFN-b CHO (start from1.1 Â 10 6 IU/ml) for 24 h, and then infected with 1000 TCID 50 of TGEV (Fig. 4A-C) or PRV (Fig. 4F-H) . The CHO-PoIFN-b cell line established in this study expresses the recombinant PoIFN-b that has the same biological function as natural porcine type I IFN.	./cache/cord-326762-t89jtjmi.txt	./txt/cord-326762-t89jtjmi.txt