id sid tid token lemma pos cord-270326-fzsczb8m 1 1 key key NN cord-270326-fzsczb8m 1 2 : : : cord-270326-fzsczb8m 2 1 cord-270326-fzsczb8 cord-270326-fzsczb8 NNP cord-270326-fzsczb8m 2 2 m m CD cord-270326-fzsczb8m 2 3 authors author NNS cord-270326-fzsczb8m 2 4 : : : cord-270326-fzsczb8m 2 5 Rubio Rubio NNP cord-270326-fzsczb8m 2 6 , , , cord-270326-fzsczb8m 2 7 Nazario Nazario NNP cord-270326-fzsczb8m 2 8 ; ; : cord-270326-fzsczb8m 2 9 de de NNP cord-270326-fzsczb8m 2 10 Felipe Felipe NNP cord-270326-fzsczb8m 2 11 , , , cord-270326-fzsczb8m 2 12 Carmen Carmen NNP cord-270326-fzsczb8m 2 13 title title NN cord-270326-fzsczb8m 2 14 : : : cord-270326-fzsczb8m 2 15 Demonstration demonstration NN cord-270326-fzsczb8m 2 16 of of IN cord-270326-fzsczb8m 2 17 the the DT cord-270326-fzsczb8m 2 18 presence presence NN cord-270326-fzsczb8m 2 19 of of IN cord-270326-fzsczb8m 2 20 a a DT cord-270326-fzsczb8m 2 21 specific specific JJ cord-270326-fzsczb8m 2 22 interferon interferon NN cord-270326-fzsczb8m 2 23 - - HYPH cord-270326-fzsczb8m 2 24 γ γ NN cord-270326-fzsczb8m 2 25 receptor receptor NN cord-270326-fzsczb8m 2 26 on on IN cord-270326-fzsczb8m 2 27 murine murine JJ cord-270326-fzsczb8m 2 28 astrocyte astrocyte JJ cord-270326-fzsczb8m 2 29 cell cell NN cord-270326-fzsczb8m 2 30 surface surface NN cord-270326-fzsczb8m 2 31 date date NN cord-270326-fzsczb8m 2 32 : : : cord-270326-fzsczb8m 2 33 1991 1991 CD cord-270326-fzsczb8m 2 34 - - SYM cord-270326-fzsczb8m 2 35 12 12 CD cord-270326-fzsczb8m 2 36 - - SYM cord-270326-fzsczb8m 2 37 31 31 CD cord-270326-fzsczb8m 2 38 journal journal NN cord-270326-fzsczb8m 2 39 : : : cord-270326-fzsczb8m 2 40 Journal Journal NNP cord-270326-fzsczb8m 2 41 of of IN cord-270326-fzsczb8m 2 42 Neuroimmunology Neuroimmunology NNP cord-270326-fzsczb8m 2 43 DOI DOI NNP cord-270326-fzsczb8m 2 44 : : : cord-270326-fzsczb8m 2 45 10.1016/0165 10.1016/0165 CD cord-270326-fzsczb8m 2 46 - - HYPH cord-270326-fzsczb8m 2 47 5728(91)90166 5728(91)90166 CD cord-270326-fzsczb8m 2 48 - - SYM cord-270326-fzsczb8m 2 49 5 5 CD cord-270326-fzsczb8m 2 50 sha sha NNP cord-270326-fzsczb8m 2 51 : : : cord-270326-fzsczb8m 2 52 fae10fe8d73478789a216595609f21e9735d310e fae10fe8d73478789a216595609f21e9735d310e NNP cord-270326-fzsczb8m 2 53 doc_id doc_id CD cord-270326-fzsczb8m 2 54 : : : cord-270326-fzsczb8m 2 55 270326 270326 CD cord-270326-fzsczb8m 2 56 cord_uid cord_uid NNS cord-270326-fzsczb8m 2 57 : : : cord-270326-fzsczb8m 2 58 fzsczb8 fzsczb8 NNP cord-270326-fzsczb8m 2 59 m m NNP cord-270326-fzsczb8m 2 60 Abstract Abstract NNP cord-270326-fzsczb8m 2 61 Interferon Interferon NNP cord-270326-fzsczb8m 2 62 gamma gamma NN cord-270326-fzsczb8m 2 63 ( ( -LRB- cord-270326-fzsczb8m 2 64 IFN IFN NNP cord-270326-fzsczb8m 2 65 - - HYPH cord-270326-fzsczb8m 2 66 γ γ NNP cord-270326-fzsczb8m 2 67 ) ) -RRB- cord-270326-fzsczb8m 2 68 is be VBZ cord-270326-fzsczb8m 2 69 a a DT cord-270326-fzsczb8m 2 70 pleiotropic pleiotropic JJ cord-270326-fzsczb8m 2 71 lymphokine lymphokine NN cord-270326-fzsczb8m 2 72 produced produce VBN cord-270326-fzsczb8m 2 73 by by IN cord-270326-fzsczb8m 2 74 T T NNP cord-270326-fzsczb8m 2 75 - - HYPH cord-270326-fzsczb8m 2 76 lymphocytes lymphocyte NNS cord-270326-fzsczb8m 2 77 which which WDT cord-270326-fzsczb8m 2 78 acts act VBZ cord-270326-fzsczb8m 2 79 as as IN cord-270326-fzsczb8m 2 80 a a DT cord-270326-fzsczb8m 2 81 soluble soluble JJ cord-270326-fzsczb8m 2 82 mediator mediator NN cord-270326-fzsczb8m 2 83 in in IN cord-270326-fzsczb8m 2 84 immunological immunological JJ cord-270326-fzsczb8m 2 85 reactions reaction NNS cord-270326-fzsczb8m 2 86 . . . cord-270326-fzsczb8m 3 1 In in IN cord-270326-fzsczb8m 3 2 addition addition NN cord-270326-fzsczb8m 3 3 to to IN cord-270326-fzsczb8m 3 4 several several JJ cord-270326-fzsczb8m 3 5 immune immune JJ cord-270326-fzsczb8m 3 6 target target NN cord-270326-fzsczb8m 3 7 cells cell NNS cord-270326-fzsczb8m 3 8 , , , cord-270326-fzsczb8m 3 9 such such JJ cord-270326-fzsczb8m 3 10 as as IN cord-270326-fzsczb8m 3 11 monocytes monocyte NNS cord-270326-fzsczb8m 3 12 and and CC cord-270326-fzsczb8m 3 13 macrophages macrophage NNS cord-270326-fzsczb8m 3 14 , , , cord-270326-fzsczb8m 3 15 it -PRON- PRP cord-270326-fzsczb8m 3 16 acts act VBZ cord-270326-fzsczb8m 3 17 on on IN cord-270326-fzsczb8m 3 18 the the DT cord-270326-fzsczb8m 3 19 principal principal JJ cord-270326-fzsczb8m 3 20 glial glial JJ cord-270326-fzsczb8m 3 21 population population NN cord-270326-fzsczb8m 3 22 , , , cord-270326-fzsczb8m 3 23 the the DT cord-270326-fzsczb8m 3 24 astrocytes astrocyte NNS cord-270326-fzsczb8m 3 25 , , , cord-270326-fzsczb8m 3 26 inducing induce VBG cord-270326-fzsczb8m 3 27 Ia Ia NNP cord-270326-fzsczb8m 3 28 antigen antigen NN cord-270326-fzsczb8m 3 29 expression expression NN cord-270326-fzsczb8m 3 30 . . . cord-270326-fzsczb8m 4 1 We -PRON- PRP cord-270326-fzsczb8m 4 2 have have VBP cord-270326-fzsczb8m 4 3 developed develop VBN cord-270326-fzsczb8m 4 4 a a DT cord-270326-fzsczb8m 4 5 binding binding JJ cord-270326-fzsczb8m 4 6 assay assay NN cord-270326-fzsczb8m 4 7 for for IN cord-270326-fzsczb8m 4 8 125I 125i CD cord-270326-fzsczb8m 4 9 - - HYPH cord-270326-fzsczb8m 4 10 labeled label VBN cord-270326-fzsczb8m 4 11 recombinant recombinant JJ cord-270326-fzsczb8m 4 12 murine murine NN cord-270326-fzsczb8m 4 13 IFN IFN NNP cord-270326-fzsczb8m 4 14 - - HYPH cord-270326-fzsczb8m 4 15 γ γ NNP cord-270326-fzsczb8m 4 16 , , , cord-270326-fzsczb8m 4 17 and and CC cord-270326-fzsczb8m 4 18 show show VB cord-270326-fzsczb8m 4 19 that that IN cord-270326-fzsczb8m 4 20 , , , cord-270326-fzsczb8m 4 21 using use VBG cord-270326-fzsczb8m 4 22 this this DT cord-270326-fzsczb8m 4 23 assay assay NN cord-270326-fzsczb8m 4 24 , , , cord-270326-fzsczb8m 4 25 IFN IFN NNP cord-270326-fzsczb8m 4 26 - - HYPH cord-270326-fzsczb8m 4 27 γ γ NNP cord-270326-fzsczb8m 4 28 interacts interact VBZ cord-270326-fzsczb8m 4 29 with with IN cord-270326-fzsczb8m 4 30 a a DT cord-270326-fzsczb8m 4 31 single single JJ cord-270326-fzsczb8m 4 32 specific specific JJ cord-270326-fzsczb8m 4 33 receptor receptor NN cord-270326-fzsczb8m 4 34 on on IN cord-270326-fzsczb8m 4 35 the the DT cord-270326-fzsczb8m 4 36 murine murine JJ cord-270326-fzsczb8m 4 37 astrocyte astrocyte NNP cord-270326-fzsczb8m 4 38 cell cell NN cord-270326-fzsczb8m 4 39 membrane membrane NN cord-270326-fzsczb8m 4 40 . . . cord-270326-fzsczb8m 5 1 The the DT cord-270326-fzsczb8m 5 2 binding binding NN cord-270326-fzsczb8m 5 3 is be VBZ cord-270326-fzsczb8m 5 4 specific specific JJ cord-270326-fzsczb8m 5 5 and and CC cord-270326-fzsczb8m 5 6 saturable saturable JJ cord-270326-fzsczb8m 5 7 and and CC cord-270326-fzsczb8m 5 8 it -PRON- PRP cord-270326-fzsczb8m 5 9 takes take VBZ cord-270326-fzsczb8m 5 10 place place NN cord-270326-fzsczb8m 5 11 with with IN cord-270326-fzsczb8m 5 12 a a DT cord-270326-fzsczb8m 5 13 K k NN cord-270326-fzsczb8m 5 14 d d NN cord-270326-fzsczb8m 5 15 = = SYM cord-270326-fzsczb8m 5 16 1.64 1.64 CD cord-270326-fzsczb8m 5 17 × × NNP cord-270326-fzsczb8m 5 18 10−9 10−9 CD cord-270326-fzsczb8m 5 19 M M NNP cord-270326-fzsczb8m 5 20 , , , cord-270326-fzsczb8m 5 21 with with IN cord-270326-fzsczb8m 5 22 11,100 11,100 CD cord-270326-fzsczb8m 5 23 receptor receptor NN cord-270326-fzsczb8m 5 24 molecules molecule NNS cord-270326-fzsczb8m 5 25 per per IN cord-270326-fzsczb8m 5 26 astrocytic astrocytic JJ cord-270326-fzsczb8m 5 27 cell cell NN cord-270326-fzsczb8m 5 28 . . . cord-270326-fzsczb8m 6 1 The the DT cord-270326-fzsczb8m 6 2 binding binding NN cord-270326-fzsczb8m 6 3 shows show NNS cord-270326-fzsczb8m 6 4 , , , cord-270326-fzsczb8m 6 5 as as IN cord-270326-fzsczb8m 6 6 for for IN cord-270326-fzsczb8m 6 7 macrophages macrophage NNS cord-270326-fzsczb8m 6 8 , , , cord-270326-fzsczb8m 6 9 species specie NNS cord-270326-fzsczb8m 6 10 specificity specificity NN cord-270326-fzsczb8m 6 11 . . . cord-270326-fzsczb8m 7 1 Using use VBG cord-270326-fzsczb8m 7 2 an an DT cord-270326-fzsczb8m 7 3 immune immune JJ cord-270326-fzsczb8m 7 4 assay assay NN cord-270326-fzsczb8m 7 5 including include VBG cord-270326-fzsczb8m 7 6 rabbit rabbit NN cord-270326-fzsczb8m 7 7 antibodies antibody NNS cord-270326-fzsczb8m 7 8 to to IN cord-270326-fzsczb8m 7 9 IFN IFN NNP cord-270326-fzsczb8m 7 10 - - HYPH cord-270326-fzsczb8m 7 11 γ γ NNP cord-270326-fzsczb8m 7 12 and and CC cord-270326-fzsczb8m 7 13 125I 125I NNP cord-270326-fzsczb8m 7 14 - - HYPH cord-270326-fzsczb8m 7 15 labeled label VBN cord-270326-fzsczb8m 7 16 protein protein NN cord-270326-fzsczb8m 7 17 A A NNP cord-270326-fzsczb8m 7 18 , , , cord-270326-fzsczb8m 7 19 we -PRON- PRP cord-270326-fzsczb8m 7 20 have have VBP cord-270326-fzsczb8m 7 21 demonstrated demonstrate VBN cord-270326-fzsczb8m 7 22 an an DT cord-270326-fzsczb8m 7 23 internalization internalization NN cord-270326-fzsczb8m 7 24 of of IN cord-270326-fzsczb8m 7 25 the the DT cord-270326-fzsczb8m 7 26 ligand ligand NN cord-270326-fzsczb8m 7 27 . . . cord-270326-fzsczb8m 8 1 This this DT cord-270326-fzsczb8m 8 2 is be VBZ cord-270326-fzsczb8m 8 3 an an DT cord-270326-fzsczb8m 8 4 energy energy NN cord-270326-fzsczb8m 8 5 - - HYPH cord-270326-fzsczb8m 8 6 dependent dependent JJ cord-270326-fzsczb8m 8 7 process process NN cord-270326-fzsczb8m 8 8 , , , cord-270326-fzsczb8m 8 9 as as IN cord-270326-fzsczb8m 8 10 around around IN cord-270326-fzsczb8m 8 11 50 50 CD cord-270326-fzsczb8m 8 12 % % NN cord-270326-fzsczb8m 8 13 of of IN cord-270326-fzsczb8m 8 14 the the DT cord-270326-fzsczb8m 8 15 bound bind VBN cord-270326-fzsczb8m 8 16 IFN IFN NNP cord-270326-fzsczb8m 8 17 - - HYPH cord-270326-fzsczb8m 8 18 γ γ NNP cord-270326-fzsczb8m 8 19 is be VBZ cord-270326-fzsczb8m 8 20 endocytosed endocytose VBN cord-270326-fzsczb8m 8 21 after after IN cord-270326-fzsczb8m 8 22 4 4 CD cord-270326-fzsczb8m 8 23 h h NN cord-270326-fzsczb8m 8 24 at at IN cord-270326-fzsczb8m 8 25 37 37 CD cord-270326-fzsczb8m 8 26 ° ° NN cord-270326-fzsczb8m 8 27 C c NN cord-270326-fzsczb8m 8 28 when when WRB cord-270326-fzsczb8m 8 29 cultures culture NNS cord-270326-fzsczb8m 8 30 are be VBP cord-270326-fzsczb8m 8 31 maintained maintain VBN cord-270326-fzsczb8m 8 32 in in IN cord-270326-fzsczb8m 8 33 complete complete JJ cord-270326-fzsczb8m 8 34 culture culture NN cord-270326-fzsczb8m 8 35 medium medium NN cord-270326-fzsczb8m 8 36 . . . cord-270326-fzsczb8m 9 1 A a DT cord-270326-fzsczb8m 9 2 paradox paradox NN cord-270326-fzsczb8m 9 3 exists exist VBZ cord-270326-fzsczb8m 9 4 in in IN cord-270326-fzsczb8m 9 5 neuroimmunology neuroimmunology NN cord-270326-fzsczb8m 9 6 as as IN cord-270326-fzsczb8m 9 7 the the DT cord-270326-fzsczb8m 9 8 central central JJ cord-270326-fzsczb8m 9 9 nervous nervous JJ cord-270326-fzsczb8m 9 10 system system NN cord-270326-fzsczb8m 9 11 ( ( -LRB- cord-270326-fzsczb8m 9 12 CNS CNS NNP cord-270326-fzsczb8m 9 13 ) ) -RRB- cord-270326-fzsczb8m 9 14 does do VBZ cord-270326-fzsczb8m 9 15 not not RB cord-270326-fzsczb8m 9 16 express express VB cord-270326-fzsczb8m 9 17 class class NN cord-270326-fzsczb8m 9 18 II II NNP cord-270326-fzsczb8m 9 19 major major JJ cord-270326-fzsczb8m 9 20 histocompatibility histocompatibility NN cord-270326-fzsczb8m 9 21 complex complex NN cord-270326-fzsczb8m 9 22 ( ( -LRB- cord-270326-fzsczb8m 9 23 MHC MHC NNP cord-270326-fzsczb8m 9 24 ) ) -RRB- cord-270326-fzsczb8m 9 25 antigens antigen NNS cord-270326-fzsczb8m 9 26 ( ( -LRB- cord-270326-fzsczb8m 9 27 Williams Williams NNP cord-270326-fzsczb8m 9 28 et et NNP cord-270326-fzsczb8m 9 29 al al NNP cord-270326-fzsczb8m 9 30 . . NNP cord-270326-fzsczb8m 9 31 , , , cord-270326-fzsczb8m 9 32 1980 1980 CD cord-270326-fzsczb8m 9 33 ) ) -RRB- cord-270326-fzsczb8m 9 34 and and CC cord-270326-fzsczb8m 9 35 pathological pathological JJ cord-270326-fzsczb8m 9 36 immune immune JJ cord-270326-fzsczb8m 9 37 reactions reaction NNS cord-270326-fzsczb8m 9 38 take take VBP cord-270326-fzsczb8m 9 39 place place NN cord-270326-fzsczb8m 9 40 within within IN cord-270326-fzsczb8m 9 41 the the DT cord-270326-fzsczb8m 9 42 CNS CNS NNP cord-270326-fzsczb8m 9 43 . . . cord-270326-fzsczb8m 10 1 This this DT cord-270326-fzsczb8m 10 2 paradox paradox NN cord-270326-fzsczb8m 10 3 can can MD cord-270326-fzsczb8m 10 4 be be VB cord-270326-fzsczb8m 10 5 resolved resolve VBN cord-270326-fzsczb8m 10 6 by by IN cord-270326-fzsczb8m 10 7 the the DT cord-270326-fzsczb8m 10 8 finding finding NN cord-270326-fzsczb8m 10 9 that that IN cord-270326-fzsczb8m 10 10 interferon interferon NN cord-270326-fzsczb8m 10 11 gamma gamma NN cord-270326-fzsczb8m 10 12 ( ( -LRB- cord-270326-fzsczb8m 10 13 IFN-7 IFN-7 NNP cord-270326-fzsczb8m 10 14 ) ) -RRB- cord-270326-fzsczb8m 10 15 and and CC cord-270326-fzsczb8m 10 16 some some DT cord-270326-fzsczb8m 10 17 viral viral JJ cord-270326-fzsczb8m 10 18 infections infection NNS cord-270326-fzsczb8m 10 19 , , , cord-270326-fzsczb8m 10 20 such such JJ cord-270326-fzsczb8m 10 21 as as IN cord-270326-fzsczb8m 10 22 those those DT cord-270326-fzsczb8m 10 23 induced induce VBN cord-270326-fzsczb8m 10 24 by by IN cord-270326-fzsczb8m 10 25 murine murine JJ cord-270326-fzsczb8m 10 26 coronavirus coronavirus NN cord-270326-fzsczb8m 10 27 ( ( -LRB- cord-270326-fzsczb8m 10 28 Suzumura Suzumura NNP cord-270326-fzsczb8m 10 29 et et NNP cord-270326-fzsczb8m 10 30 al al NNP cord-270326-fzsczb8m 10 31 . . NNP cord-270326-fzsczb8m 10 32 , , , cord-270326-fzsczb8m 10 33 1986 1986 CD cord-270326-fzsczb8m 10 34 ) ) -RRB- cord-270326-fzsczb8m 10 35 , , , cord-270326-fzsczb8m 10 36 Theiler Theiler NNP cord-270326-fzsczb8m 10 37 's 's POS cord-270326-fzsczb8m 10 38 murine murine JJ cord-270326-fzsczb8m 10 39 encephalomyelitis encephalomyelitis NN cord-270326-fzsczb8m 10 40 virus virus NN cord-270326-fzsczb8m 10 41 ( ( -LRB- cord-270326-fzsczb8m 10 42 Rodriguez Rodriguez NNP cord-270326-fzsczb8m 10 43 et et NNP cord-270326-fzsczb8m 10 44 al al NNP cord-270326-fzsczb8m 10 45 . . NNP cord-270326-fzsczb8m 10 46 , , , cord-270326-fzsczb8m 10 47 1987 1987 CD cord-270326-fzsczb8m 10 48 ) ) -RRB- cord-270326-fzsczb8m 10 49 or or CC cord-270326-fzsczb8m 10 50 neurotropic neurotropic NNP cord-270326-fzsczb8m 10 51 routine routine JJ cord-270326-fzsczb8m 10 52 hepatitis hepatitis NN cord-270326-fzsczb8m 10 53 virus virus NN cord-270326-fzsczb8m 10 54 ( ( -LRB- cord-270326-fzsczb8m 10 55 Massa Massa NNP cord-270326-fzsczb8m 10 56 et et FW cord-270326-fzsczb8m 10 57 al al NNP cord-270326-fzsczb8m 10 58 . . NNP cord-270326-fzsczb8m 10 59 , , , cord-270326-fzsczb8m 10 60 1986 1986 CD cord-270326-fzsczb8m 10 61 ) ) -RRB- cord-270326-fzsczb8m 10 62 can can MD cord-270326-fzsczb8m 10 63 induce induce VB cord-270326-fzsczb8m 10 64 the the DT cord-270326-fzsczb8m 10 65 expression expression NN cord-270326-fzsczb8m 10 66 of of IN cord-270326-fzsczb8m 10 67 new new JJ cord-270326-fzsczb8m 10 68 antigens antigen NNS cord-270326-fzsczb8m 10 69 in in IN cord-270326-fzsczb8m 10 70 astrocytes astrocyte NNS cord-270326-fzsczb8m 10 71 . . . cord-270326-fzsczb8m 11 1 Several several JJ cord-270326-fzsczb8m 11 2 studies study NNS cord-270326-fzsczb8m 11 3 have have VBP cord-270326-fzsczb8m 11 4 shown show VBN cord-270326-fzsczb8m 11 5 that that IN cord-270326-fzsczb8m 11 6 IFN IFN NNP cord-270326-fzsczb8m 11 7 - - HYPH cord-270326-fzsczb8m 11 8 y y NNP cord-270326-fzsczb8m 11 9 , , , cord-270326-fzsczb8m 11 10 also also RB cord-270326-fzsczb8m 11 11 known know VBN cord-270326-fzsczb8m 11 12 as as IN cord-270326-fzsczb8m 11 13 immune immune JJ cord-270326-fzsczb8m 11 14 interferon interferon NN cord-270326-fzsczb8m 11 15 , , , cord-270326-fzsczb8m 11 16 induces induce VBZ cord-270326-fzsczb8m 11 17 the the DT cord-270326-fzsczb8m 11 18 expression expression NN cord-270326-fzsczb8m 11 19 of of IN cord-270326-fzsczb8m 11 20 class class NN cord-270326-fzsczb8m 11 21 II II NNP cord-270326-fzsczb8m 11 22 MHC MHC NNP cord-270326-fzsczb8m 11 23 antigens antigen NNS cord-270326-fzsczb8m 11 24 in in IN cord-270326-fzsczb8m 11 25 macrophages macrophage NNS cord-270326-fzsczb8m 11 26 ( ( -LRB- cord-270326-fzsczb8m 11 27 Steeg Steeg NNP cord-270326-fzsczb8m 11 28 et et FW cord-270326-fzsczb8m 11 29 al al NNP cord-270326-fzsczb8m 11 30 . . NNP cord-270326-fzsczb8m 11 31 , , , cord-270326-fzsczb8m 11 32 1982 1982 CD cord-270326-fzsczb8m 11 33 ) ) -RRB- cord-270326-fzsczb8m 11 34 and and CC cord-270326-fzsczb8m 11 35 astrocytes astrocyte NNS cord-270326-fzsczb8m 11 36 ( ( -LRB- cord-270326-fzsczb8m 11 37 Hirsch Hirsch NNP cord-270326-fzsczb8m 11 38 et et NNP cord-270326-fzsczb8m 11 39 al al NNP cord-270326-fzsczb8m 11 40 . . NNP cord-270326-fzsczb8m 11 41 , , , cord-270326-fzsczb8m 11 42 1983 1983 CD cord-270326-fzsczb8m 11 43 ; ; . cord-270326-fzsczb8m 12 1 Wong Wong NNP cord-270326-fzsczb8m 12 2 et et NNP cord-270326-fzsczb8m 12 3 al al NNP cord-270326-fzsczb8m 12 4 . . NNP cord-270326-fzsczb8m 12 5 , , , cord-270326-fzsczb8m 12 6 1985 1985 CD cord-270326-fzsczb8m 12 7 ) ) -RRB- cord-270326-fzsczb8m 12 8 . . . cord-270326-fzsczb8m 13 1 The the DT cord-270326-fzsczb8m 13 2 fact fact NN cord-270326-fzsczb8m 13 3 that that IN cord-270326-fzsczb8m 13 4 a a DT cord-270326-fzsczb8m 13 5 T t NN cord-270326-fzsczb8m 13 6 - - HYPH cord-270326-fzsczb8m 13 7 lymphocyte lymphocyte NN cord-270326-fzsczb8m 13 8 lymphokine lymphokine NN cord-270326-fzsczb8m 13 9 produces produce VBZ cord-270326-fzsczb8m 13 10 such such PDT cord-270326-fzsczb8m 13 11 a a DT cord-270326-fzsczb8m 13 12 striking striking JJ cord-270326-fzsczb8m 13 13 effect effect NN cord-270326-fzsczb8m 13 14 on on IN cord-270326-fzsczb8m 13 15 glial glial JJ cord-270326-fzsczb8m 13 16 cells cell NNS cord-270326-fzsczb8m 13 17 may may MD cord-270326-fzsczb8m 13 18 have have VB cord-270326-fzsczb8m 13 19 high high JJ cord-270326-fzsczb8m 13 20 functional functional JJ cord-270326-fzsczb8m 13 21 significance significance NN cord-270326-fzsczb8m 13 22 . . . cord-270326-fzsczb8m 14 1 It -PRON- PRP cord-270326-fzsczb8m 14 2 has have VBZ cord-270326-fzsczb8m 14 3 been be VBN cord-270326-fzsczb8m 14 4 demonstrated demonstrate VBN cord-270326-fzsczb8m 14 5 that that IN cord-270326-fzsczb8m 14 6 IFN IFN NNP cord-270326-fzsczb8m 14 7 - - HYPH cord-270326-fzsczb8m 14 8 y y NNP cord-270326-fzsczb8m 14 9 induces induce VBZ cord-270326-fzsczb8m 14 10 la la NNP cord-270326-fzsczb8m 14 11 antigen antigen NNP cord-270326-fzsczb8m 14 12 expression expression NN cord-270326-fzsczb8m 14 13 on on IN cord-270326-fzsczb8m 14 14 astrocytes astrocyte NNS cord-270326-fzsczb8m 14 15 ( ( -LRB- cord-270326-fzsczb8m 14 16 Fierz Fierz NNP cord-270326-fzsczb8m 14 17 et et FW cord-270326-fzsczb8m 14 18 al al NNP cord-270326-fzsczb8m 14 19 . . NNP cord-270326-fzsczb8m 14 20 , , , cord-270326-fzsczb8m 14 21 1985 1985 CD cord-270326-fzsczb8m 14 22 ) ) -RRB- cord-270326-fzsczb8m 14 23 and and CC cord-270326-fzsczb8m 14 24 that that IN cord-270326-fzsczb8m 14 25 astrocytes astrocyte NNS cord-270326-fzsczb8m 14 26 activated activate VBN cord-270326-fzsczb8m 14 27 in in IN cord-270326-fzsczb8m 14 28 this this DT cord-270326-fzsczb8m 14 29 manner manner NN cord-270326-fzsczb8m 14 30 can can MD cord-270326-fzsczb8m 14 31 present present VB cord-270326-fzsczb8m 14 32 antigens antigen NNS cord-270326-fzsczb8m 14 33 such such JJ cord-270326-fzsczb8m 14 34 as as IN cord-270326-fzsczb8m 14 35 myelin myelin JJ cord-270326-fzsczb8m 14 36 basic basic JJ cord-270326-fzsczb8m 14 37 protein protein NN cord-270326-fzsczb8m 14 38 to to IN cord-270326-fzsczb8m 14 39 Tlymphocytes Tlymphocytes NNP cord-270326-fzsczb8m 14 40 ( ( -LRB- cord-270326-fzsczb8m 14 41 Fontana Fontana NNP cord-270326-fzsczb8m 14 42 et et NNP cord-270326-fzsczb8m 14 43 al al NNP cord-270326-fzsczb8m 14 44 . . NNP cord-270326-fzsczb8m 14 45 , , , cord-270326-fzsczb8m 14 46 1984 1984 CD cord-270326-fzsczb8m 14 47 ) ) -RRB- cord-270326-fzsczb8m 14 48 . . . cord-270326-fzsczb8m 15 1 These these DT cord-270326-fzsczb8m 15 2 autoaggressive autoaggressive JJ cord-270326-fzsczb8m 15 3 T t NN cord-270326-fzsczb8m 15 4 - - HYPH cord-270326-fzsczb8m 15 5 cells cell NNS cord-270326-fzsczb8m 15 6 can can MD cord-270326-fzsczb8m 15 7 lysc lysc FW cord-270326-fzsczb8m 15 8 presenting present VBG cord-270326-fzsczb8m 15 9 astrocytes astrocyte NNS cord-270326-fzsczb8m 15 10 ( ( -LRB- cord-270326-fzsczb8m 15 11 Sun Sun NNP cord-270326-fzsczb8m 15 12 and and CC cord-270326-fzsczb8m 15 13 Wekerle Wekerle NNP cord-270326-fzsczb8m 15 14 , , , cord-270326-fzsczb8m 15 15 1986 1986 CD cord-270326-fzsczb8m 15 16 ) ) -RRB- cord-270326-fzsczb8m 15 17 . . . cord-270326-fzsczb8m 16 1 Conversely conversely RB cord-270326-fzsczb8m 16 2 , , , cord-270326-fzsczb8m 16 3 astrocytes astrocyte NNS cord-270326-fzsczb8m 16 4 do do VBP cord-270326-fzsczb8m 16 5 not not RB cord-270326-fzsczb8m 16 6 produce produce VB cord-270326-fzsczb8m 16 7 IFN-7 IFN-7 NNP cord-270326-fzsczb8m 16 8 , , , cord-270326-fzsczb8m 16 9 even even RB cord-270326-fzsczb8m 16 10 when when WRB cord-270326-fzsczb8m 16 11 subjected subject VBN cord-270326-fzsczb8m 16 12 to to IN cord-270326-fzsczb8m 16 13 a a DT cord-270326-fzsczb8m 16 14 superinduction superinduction NN cord-270326-fzsczb8m 16 15 regimen regimen NN cord-270326-fzsczb8m 16 16 ( ( -LRB- cord-270326-fzsczb8m 16 17 Tedeschi Tedeschi NNP cord-270326-fzsczb8m 16 18 et et NNP cord-270326-fzsczb8m 16 19 al al NNP cord-270326-fzsczb8m 16 20 . . NNP cord-270326-fzsczb8m 16 21 , , , cord-270326-fzsczb8m 16 22 1986 1986 CD cord-270326-fzsczb8m 16 23 ) ) -RRB- cord-270326-fzsczb8m 16 24 . . . cord-270326-fzsczb8m 17 1 We -PRON- PRP cord-270326-fzsczb8m 17 2 have have VBP cord-270326-fzsczb8m 17 3 undertaken undertake VBN cord-270326-fzsczb8m 17 4 the the DT cord-270326-fzsczb8m 17 5 study study NN cord-270326-fzsczb8m 17 6 of of IN cord-270326-fzsczb8m 17 7 the the DT cord-270326-fzsczb8m 17 8 interaction interaction NN cord-270326-fzsczb8m 17 9 between between IN cord-270326-fzsczb8m 17 10 recombinant recombinant JJ cord-270326-fzsczb8m 17 11 IFN IFN NNP cord-270326-fzsczb8m 17 12 - - HYPH cord-270326-fzsczb8m 17 13 T T NNP cord-270326-fzsczb8m 17 14 and and CC cord-270326-fzsczb8m 17 15 its -PRON- PRP$ cord-270326-fzsczb8m 17 16 receptor receptor NN cord-270326-fzsczb8m 17 17 on on IN cord-270326-fzsczb8m 17 18 the the DT cord-270326-fzsczb8m 17 19 murine murine JJ cord-270326-fzsczb8m 17 20 astrocyte astrocyte NNP cord-270326-fzsczb8m 17 21 cellular cellular JJ cord-270326-fzsczb8m 17 22 membrane membrane NN cord-270326-fzsczb8m 17 23 from from IN cord-270326-fzsczb8m 17 24 a a DT cord-270326-fzsczb8m 17 25 physicochemical physicochemical JJ cord-270326-fzsczb8m 17 26 point point NN cord-270326-fzsczb8m 17 27 of of IN cord-270326-fzsczb8m 17 28 view view NN cord-270326-fzsczb8m 17 29 . . . cord-270326-fzsczb8m 18 1 Here here RB cord-270326-fzsczb8m 18 2 we -PRON- PRP cord-270326-fzsczb8m 18 3 describe describe VBP cord-270326-fzsczb8m 18 4 in in IN cord-270326-fzsczb8m 18 5 detail detail NN cord-270326-fzsczb8m 18 6 the the DT cord-270326-fzsczb8m 18 7 binding bind VBG cord-270326-fzsczb8m 18 8 characteristics characteristic NNS cord-270326-fzsczb8m 18 9 of of IN cord-270326-fzsczb8m 18 10 this this DT cord-270326-fzsczb8m 18 11 important important JJ cord-270326-fzsczb8m 18 12 neuroimmunological neuroimmunological JJ cord-270326-fzsczb8m 18 13 reaction reaction NN cord-270326-fzsczb8m 18 14 . . . cord-270326-fzsczb8m 19 1 Recombinant recombinant JJ cord-270326-fzsczb8m 19 2 mouse mouse NN cord-270326-fzsczb8m 19 3 gamma gamma NN cord-270326-fzsczb8m 19 4 interferon interferon NN cord-270326-fzsczb8m 19 5 , , , cord-270326-fzsczb8m 19 6 free free JJ cord-270326-fzsczb8m 19 7 of of IN cord-270326-fzsczb8m 19 8 protein protein NN cord-270326-fzsczb8m 19 9 stabilizers stabilizer NNS cord-270326-fzsczb8m 19 10 was be VBD cord-270326-fzsczb8m 19 11 purchased purchase VBN cord-270326-fzsczb8m 19 12 from from IN cord-270326-fzsczb8m 19 13 Holland Holland NNP cord-270326-fzsczb8m 19 14 Biotechnology Biotechnology NNP cord-270326-fzsczb8m 19 15 , , , cord-270326-fzsczb8m 19 16 Leiden Leiden NNP cord-270326-fzsczb8m 19 17 , , , cord-270326-fzsczb8m 19 18 The the DT cord-270326-fzsczb8m 19 19 Netherlands Netherlands NNP cord-270326-fzsczb8m 19 20 and and CC cord-270326-fzsczb8m 19 21 was be VBD cord-270326-fzsczb8m 19 22 labeled label VBN cord-270326-fzsczb8m 19 23 with with IN cord-270326-fzsczb8m 19 24 J25I J25I NNP cord-270326-fzsczb8m 20 1 ( ( -LRB- cord-270326-fzsczb8m 20 2 Amersham Amersham NNP cord-270326-fzsczb8m 20 3 International International NNP cord-270326-fzsczb8m 20 4 , , , cord-270326-fzsczb8m 20 5 U.K. U.K. NNP cord-270326-fzsczb8m 20 6 ) ) -RRB- cord-270326-fzsczb8m 21 1 utilizing utilize VBG cord-270326-fzsczb8m 21 2 the the DT cord-270326-fzsczb8m 21 3 enzymobead enzymobead NNP cord-270326-fzsczb8m 21 4 iodination iodination NN cord-270326-fzsczb8m 21 5 lactoperoxidase lactoperoxidase NNP cord-270326-fzsczb8m 21 6 reagent reagent NNP cord-270326-fzsczb8m 21 7 ( ( -LRB- cord-270326-fzsczb8m 21 8 Bio Bio NNP cord-270326-fzsczb8m 21 9 - - HYPH cord-270326-fzsczb8m 21 10 Rad Rad NNP cord-270326-fzsczb8m 21 11 , , , cord-270326-fzsczb8m 21 12 Richmond Richmond NNP cord-270326-fzsczb8m 21 13 , , , cord-270326-fzsczb8m 21 14 CA CA NNP cord-270326-fzsczb8m 21 15 , , , cord-270326-fzsczb8m 21 16 U.S.A. U.S.A. NNP cord-270326-fzsczb8m 21 17 ) ) -RRB- cord-270326-fzsczb8m 21 18 ( ( -LRB- cord-270326-fzsczb8m 21 19 Marchalonis Marchalonis NNP cord-270326-fzsczb8m 21 20 , , , cord-270326-fzsczb8m 21 21 1969 1969 CD cord-270326-fzsczb8m 21 22 ) ) -RRB- cord-270326-fzsczb8m 21 23 . . . cord-270326-fzsczb8m 22 1 The the DT cord-270326-fzsczb8m 22 2 ~25I ~25i JJ cord-270326-fzsczb8m 22 3 - - HYPH cord-270326-fzsczb8m 22 4 rIFN-7 rIFN-7 JJS cord-270326-fzsczb8m 22 5 and and CC cord-270326-fzsczb8m 22 6 free free JJ cord-270326-fzsczb8m 22 7 iodine iodine NN cord-270326-fzsczb8m 22 8 were be VBD cord-270326-fzsczb8m 22 9 separated separate VBN cord-270326-fzsczb8m 22 10 on on IN cord-270326-fzsczb8m 22 11 a a DT cord-270326-fzsczb8m 22 12 disposable disposable JJ cord-270326-fzsczb8m 22 13 PD-10 PD-10 NNP cord-270326-fzsczb8m 22 14 Sephadex Sephadex NNP cord-270326-fzsczb8m 22 15 G-25 G-25 NNP cord-270326-fzsczb8m 22 16 M M NNP cord-270326-fzsczb8m 22 17 column column NN cord-270326-fzsczb8m 22 18 ( ( -LRB- cord-270326-fzsczb8m 22 19 Pharmacia Pharmacia NNP cord-270326-fzsczb8m 22 20 , , , cord-270326-fzsczb8m 22 21 Uppsala Uppsala NNP cord-270326-fzsczb8m 22 22 , , , cord-270326-fzsczb8m 22 23 Sweden Sweden NNP cord-270326-fzsczb8m 22 24 ) ) -RRB- cord-270326-fzsczb8m 22 25 . . . cord-270326-fzsczb8m 23 1 The the DT cord-270326-fzsczb8m 23 2 specific specific JJ cord-270326-fzsczb8m 23 3 activity activity NN cord-270326-fzsczb8m 23 4 obtained obtain VBN cord-270326-fzsczb8m 23 5 was be VBD cord-270326-fzsczb8m 23 6 203 203 CD cord-270326-fzsczb8m 23 7 Ci ci NN cord-270326-fzsczb8m 23 8 / / SYM cord-270326-fzsczb8m 23 9 mmol mmol NN cord-270326-fzsczb8m 23 10 and and CC cord-270326-fzsczb8m 23 11 was be VBD cord-270326-fzsczb8m 23 12 96 96 CD cord-270326-fzsczb8m 23 13 % % NN cord-270326-fzsczb8m 23 14 trichloroacetic trichloroacetic JJ cord-270326-fzsczb8m 23 15 acid acid NN cord-270326-fzsczb8m 23 16 ( ( -LRB- cord-270326-fzsczb8m 23 17 TCA TCA NNP cord-270326-fzsczb8m 23 18 ) ) -RRB- cord-270326-fzsczb8m 23 19 precipitable precipitable NN cord-270326-fzsczb8m 23 20 . . . cord-270326-fzsczb8m 24 1 The the DT cord-270326-fzsczb8m 24 2 iodinated iodinate VBN cord-270326-fzsczb8m 24 3 rIFN rifn NN cord-270326-fzsczb8m 24 4 - - HYPH cord-270326-fzsczb8m 24 5 T T NNP cord-270326-fzsczb8m 24 6 migrated migrate VBD cord-270326-fzsczb8m 24 7 mostly mostly RB cord-270326-fzsczb8m 24 8 as as IN cord-270326-fzsczb8m 24 9 a a DT cord-270326-fzsczb8m 24 10 single single JJ cord-270326-fzsczb8m 24 11 peak peak NN cord-270326-fzsczb8m 24 12 with with IN cord-270326-fzsczb8m 24 13 an an DT cord-270326-fzsczb8m 24 14 apparent apparent JJ cord-270326-fzsczb8m 24 15 molecular molecular JJ cord-270326-fzsczb8m 24 16 weight weight NN cord-270326-fzsczb8m 24 17 of of IN cord-270326-fzsczb8m 24 18 20,000 20,000 CD cord-270326-fzsczb8m 24 19 when when WRB cord-270326-fzsczb8m 24 20 analyzed analyze VBN cord-270326-fzsczb8m 24 21 by by IN cord-270326-fzsczb8m 24 22 sodium sodium NN cord-270326-fzsczb8m 24 23 dodecyl dodecyl NNP cord-270326-fzsczb8m 24 24 sulfate sulfate NN cord-270326-fzsczb8m 24 25 - - HYPH cord-270326-fzsczb8m 24 26 polyacrylamide polyacrylamide NN cord-270326-fzsczb8m 24 27 gel gel NN cord-270326-fzsczb8m 24 28 electrophoresis electrophoresis NN cord-270326-fzsczb8m 24 29 slabs slab NNS cord-270326-fzsczb8m 24 30 . . . cord-270326-fzsczb8m 25 1 Protein protein VB cord-270326-fzsczb8m 25 2 A A NNP cord-270326-fzsczb8m 25 3 from from IN cord-270326-fzsczb8m 25 4 Staphylococcus Staphylococcus NNP cord-270326-fzsczb8m 25 5 aureus aureus NN cord-270326-fzsczb8m 25 6 ( ( -LRB- cord-270326-fzsczb8m 25 7 Sigma Sigma NNP cord-270326-fzsczb8m 25 8 Chemical Chemical NNP cord-270326-fzsczb8m 25 9 Co. Co. NNP cord-270326-fzsczb8m 25 10 , , , cord-270326-fzsczb8m 25 11 St. St. NNP cord-270326-fzsczb8m 25 12 Louis Louis NNP cord-270326-fzsczb8m 25 13 , , , cord-270326-fzsczb8m 25 14 MO MO NNP cord-270326-fzsczb8m 25 15 , , , cord-270326-fzsczb8m 25 16 U.S.A. U.S.A. NNP cord-270326-fzsczb8m 25 17 ) ) -RRB- cord-270326-fzsczb8m 25 18 was be VBD cord-270326-fzsczb8m 25 19 labeled label VBN cord-270326-fzsczb8m 25 20 by by IN cord-270326-fzsczb8m 25 21 the the DT cord-270326-fzsczb8m 25 22 chloramine chloramine NN cord-270326-fzsczb8m 25 23 T t NN cord-270326-fzsczb8m 25 24 method method NN cord-270326-fzsczb8m 25 25 ( ( -LRB- cord-270326-fzsczb8m 25 26 Greenwood Greenwood NNP cord-270326-fzsczb8m 25 27 et et NNP cord-270326-fzsczb8m 25 28 al al NNP cord-270326-fzsczb8m 25 29 . . NNP cord-270326-fzsczb8m 25 30 , , , cord-270326-fzsczb8m 25 31 1963 1963 CD cord-270326-fzsczb8m 25 32 ) ) -RRB- cord-270326-fzsczb8m 25 33 . . . cord-270326-fzsczb8m 26 1 Astrocyte Astrocyte NNP cord-270326-fzsczb8m 26 2 cultures culture NNS cord-270326-fzsczb8m 26 3 were be VBD cord-270326-fzsczb8m 26 4 prepared prepare VBN cord-270326-fzsczb8m 26 5 by by IN cord-270326-fzsczb8m 26 6 mechanical mechanical JJ cord-270326-fzsczb8m 26 7 dissociation dissociation NN cord-270326-fzsczb8m 26 8 of of IN cord-270326-fzsczb8m 26 9 the the DT cord-270326-fzsczb8m 26 10 cerebral cerebral JJ cord-270326-fzsczb8m 26 11 cortex cortex NN cord-270326-fzsczb8m 26 12 from from IN cord-270326-fzsczb8m 26 13 newborn newborn JJ cord-270326-fzsczb8m 26 14 Wistar Wistar NNP cord-270326-fzsczb8m 26 15 rats rat NNS cord-270326-fzsczb8m 26 16 or or CC cord-270326-fzsczb8m 26 17 SJL SJL NNP cord-270326-fzsczb8m 26 18 / / SYM cord-270326-fzsczb8m 26 19 J J NNP cord-270326-fzsczb8m 26 20 mice mouse NNS cord-270326-fzsczb8m 26 21 . . . cord-270326-fzsczb8m 27 1 The the DT cord-270326-fzsczb8m 27 2 cortex cortex NN cord-270326-fzsczb8m 27 3 was be VBD cord-270326-fzsczb8m 27 4 isolated isolate VBN cord-270326-fzsczb8m 27 5 under under IN cord-270326-fzsczb8m 27 6 a a DT cord-270326-fzsczb8m 27 7 dissecting dissect VBG cord-270326-fzsczb8m 27 8 microscope microscope NN cord-270326-fzsczb8m 27 9 and and CC cord-270326-fzsczb8m 27 10 carefully carefully RB cord-270326-fzsczb8m 27 11 cleaned clean VBN cord-270326-fzsczb8m 27 12 of of IN cord-270326-fzsczb8m 27 13 choroid choroid JJ cord-270326-fzsczb8m 27 14 plexus plexus NN cord-270326-fzsczb8m 27 15 and and CC cord-270326-fzsczb8m 27 16 meninges meninge NNS cord-270326-fzsczb8m 27 17 . . . cord-270326-fzsczb8m 28 1 Cell cell NN cord-270326-fzsczb8m 28 2 suspensions suspension NNS cord-270326-fzsczb8m 28 3 were be VBD cord-270326-fzsczb8m 28 4 filtered filter VBN cord-270326-fzsczb8m 28 5 through through IN cord-270326-fzsczb8m 28 6 80 80 CD cord-270326-fzsczb8m 28 7 /zm /zm DT cord-270326-fzsczb8m 28 8 pore pore NN cord-270326-fzsczb8m 28 9 size size NN cord-270326-fzsczb8m 28 10 mesh mesh NN cord-270326-fzsczb8m 28 11 into into IN cord-270326-fzsczb8m 28 12 Dulbecco Dulbecco NNP cord-270326-fzsczb8m 28 13 's 's POS cord-270326-fzsczb8m 28 14 modified modify VBN cord-270326-fzsczb8m 28 15 Eagle Eagle NNP cord-270326-fzsczb8m 28 16 medium medium NN cord-270326-fzsczb8m 28 17 ( ( -LRB- cord-270326-fzsczb8m 28 18 DMEM DMEM NNP cord-270326-fzsczb8m 28 19 ) ) -RRB- cord-270326-fzsczb8m 28 20 containing contain VBG cord-270326-fzsczb8m 28 21 10 10 CD cord-270326-fzsczb8m 28 22 % % NN cord-270326-fzsczb8m 28 23 fetal fetal JJ cord-270326-fzsczb8m 28 24 calf calf NN cord-270326-fzsczb8m 28 25 serum serum NN cord-270326-fzsczb8m 28 26 ( ( -LRB- cord-270326-fzsczb8m 28 27 FCS FCS NNP cord-270326-fzsczb8m 28 28 ) ) -RRB- cord-270326-fzsczb8m 28 29 and and CC cord-270326-fzsczb8m 28 30 gentamicin gentamicin NNP cord-270326-fzsczb8m 28 31 ( ( -LRB- cord-270326-fzsczb8m 28 32 Flow Flow NNP cord-270326-fzsczb8m 28 33 Laboratories Laboratories NNPS cord-270326-fzsczb8m 28 34 , , , cord-270326-fzsczb8m 28 35 U.K. U.K. NNP cord-270326-fzsczb8m 28 36 ) ) -RRB- cord-270326-fzsczb8m 28 37 . . . cord-270326-fzsczb8m 29 1 After after IN cord-270326-fzsczb8m 29 2 centrifugation centrifugation NN cord-270326-fzsczb8m 29 3 , , , cord-270326-fzsczb8m 29 4 cells cell NNS cord-270326-fzsczb8m 29 5 were be VBD cord-270326-fzsczb8m 29 6 filtered filter VBN cord-270326-fzsczb8m 29 7 through through IN cord-270326-fzsczb8m 29 8 a a DT cord-270326-fzsczb8m 29 9 20 20 CD cord-270326-fzsczb8m 29 10 # # NN cord-270326-fzsczb8m 29 11 m m NN cord-270326-fzsczb8m 29 12 mesh mesh NN cord-270326-fzsczb8m 29 13 sieve sieve NN cord-270326-fzsczb8m 29 14 , , , cord-270326-fzsczb8m 29 15 plated plate VBN cord-270326-fzsczb8m 29 16 in in IN cord-270326-fzsczb8m 29 17 75 75 CD cord-270326-fzsczb8m 29 18 cm cm NN cord-270326-fzsczb8m 29 19 -~ -~ NN cord-270326-fzsczb8m 29 20 tissue tissue NN cord-270326-fzsczb8m 29 21 culture culture NN cord-270326-fzsczb8m 29 22 flasks flask NNS cord-270326-fzsczb8m 29 23 ( ( -LRB- cord-270326-fzsczb8m 29 24 Costar Costar NNP cord-270326-fzsczb8m 29 25 , , , cord-270326-fzsczb8m 29 26 Cambridge Cambridge NNP cord-270326-fzsczb8m 29 27 , , , cord-270326-fzsczb8m 29 28 MA MA NNP cord-270326-fzsczb8m 29 29 , , , cord-270326-fzsczb8m 29 30 U.S.A. U.S.A. NNP cord-270326-fzsczb8m 29 31 ) ) -RRB- cord-270326-fzsczb8m 29 32 and and CC cord-270326-fzsczb8m 29 33 cultured culture VBN cord-270326-fzsczb8m 29 34 at at IN cord-270326-fzsczb8m 29 35 37 37 CD cord-270326-fzsczb8m 29 36 ° ° NN cord-270326-fzsczb8m 29 37 C. c. NN cord-270326-fzsczb8m 30 1 The the DT cord-270326-fzsczb8m 30 2 medium medium NN cord-270326-fzsczb8m 30 3 was be VBD cord-270326-fzsczb8m 30 4 changed change VBN cord-270326-fzsczb8m 30 5 after after IN cord-270326-fzsczb8m 30 6 4 4 CD cord-270326-fzsczb8m 30 7 days day NNS cord-270326-fzsczb8m 30 8 of of IN cord-270326-fzsczb8m 30 9 culture culture NN cord-270326-fzsczb8m 30 10 and and CC cord-270326-fzsczb8m 30 11 , , , cord-270326-fzsczb8m 30 12 subsequently subsequently RB cord-270326-fzsczb8m 30 13 , , , cord-270326-fzsczb8m 30 14 3 3 CD cord-270326-fzsczb8m 30 15 times time NNS cord-270326-fzsczb8m 30 16 a a DT cord-270326-fzsczb8m 30 17 week week NN cord-270326-fzsczb8m 30 18 for for IN cord-270326-fzsczb8m 30 19 the the DT cord-270326-fzsczb8m 30 20 entire entire JJ cord-270326-fzsczb8m 30 21 culture culture NN cord-270326-fzsczb8m 30 22 period period NN cord-270326-fzsczb8m 30 23 . . . cord-270326-fzsczb8m 31 1 Cultures culture NNS cord-270326-fzsczb8m 31 2 were be VBD cord-270326-fzsczb8m 31 3 enriched enrich VBN cord-270326-fzsczb8m 31 4 in in IN cord-270326-fzsczb8m 31 5 astrocytes astrocyte NNS cord-270326-fzsczb8m 31 6 by by IN cord-270326-fzsczb8m 31 7 removal removal NN cord-270326-fzsczb8m 31 8 of of IN cord-270326-fzsczb8m 31 9 less less JJR cord-270326-fzsczb8m 31 10 adherent adherent JJ cord-270326-fzsczb8m 31 11 oligodendrocytes oligodendrocyte NNS cord-270326-fzsczb8m 31 12 by by IN cord-270326-fzsczb8m 31 13 shaking shake VBG cord-270326-fzsczb8m 31 14 for for IN cord-270326-fzsczb8m 31 15 18 18 CD cord-270326-fzsczb8m 31 16 h h NN cord-270326-fzsczb8m 31 17 at at IN cord-270326-fzsczb8m 31 18 37 37 CD cord-270326-fzsczb8m 31 19 ° ° NN cord-270326-fzsczb8m 31 20 C c NN cord-270326-fzsczb8m 31 21 and and CC cord-270326-fzsczb8m 31 22 250 250 CD cord-270326-fzsczb8m 31 23 rpm rpm NN cord-270326-fzsczb8m 31 24 in in IN cord-270326-fzsczb8m 31 25 an an DT cord-270326-fzsczb8m 31 26 orbital orbital JJ cord-270326-fzsczb8m 31 27 shaker shaker NN cord-270326-fzsczb8m 31 28 . . . cord-270326-fzsczb8m 32 1 Cellular cellular JJ cord-270326-fzsczb8m 32 2 confluence confluence NN cord-270326-fzsczb8m 32 3 was be VBD cord-270326-fzsczb8m 32 4 observed observe VBN cord-270326-fzsczb8m 32 5 10 10 CD cord-270326-fzsczb8m 32 6 days day NNS cord-270326-fzsczb8m 32 7 after after IN cord-270326-fzsczb8m 32 8 plating plate VBG cord-270326-fzsczb8m 32 9 with with IN cord-270326-fzsczb8m 32 10 a a DT cord-270326-fzsczb8m 32 11 polygonal polygonal JJ cord-270326-fzsczb8m 32 12 fiat fiat JJ cord-270326-fzsczb8m 32 13 cell cell NN cord-270326-fzsczb8m 32 14 morphology morphology NN cord-270326-fzsczb8m 32 15 . . . cord-270326-fzsczb8m 33 1 The the DT cord-270326-fzsczb8m 33 2 content content NN cord-270326-fzsczb8m 33 3 of of IN cord-270326-fzsczb8m 33 4 a a DT cord-270326-fzsczb8m 33 5 mean mean NN cord-270326-fzsczb8m 33 6 of of IN cord-270326-fzsczb8m 33 7 98.4 98.4 CD cord-270326-fzsczb8m 33 8 % % NN cord-270326-fzsczb8m 33 9 astrocytes astrocyte NNS cord-270326-fzsczb8m 33 10 was be VBD cord-270326-fzsczb8m 33 11 confirmed confirm VBN cord-270326-fzsczb8m 33 12 by by IN cord-270326-fzsczb8m 33 13 indirect indirect JJ cord-270326-fzsczb8m 33 14 immunofluorescence immunofluorescence NN cord-270326-fzsczb8m 33 15 staining staining NN cord-270326-fzsczb8m 33 16 of of IN cord-270326-fzsczb8m 33 17 methanol methanol NN cord-270326-fzsczb8m 33 18 - - HYPH cord-270326-fzsczb8m 33 19 fixed fix VBN cord-270326-fzsczb8m 33 20 cultures culture NNS cord-270326-fzsczb8m 33 21 using use VBG cord-270326-fzsczb8m 33 22 rabbit rabbit NN cord-270326-fzsczb8m 33 23 anti anti NNP cord-270326-fzsczb8m 33 24 - - JJ cord-270326-fzsczb8m 33 25 gliaI gliai JJ cord-270326-fzsczb8m 33 26 fibrillary fibrillary JJ cord-270326-fzsczb8m 33 27 acidic acidic JJ cord-270326-fzsczb8m 33 28 protein protein NN cord-270326-fzsczb8m 33 29 ( ( -LRB- cord-270326-fzsczb8m 33 30 GFAP GFAP NNP cord-270326-fzsczb8m 33 31 ) ) -RRB- cord-270326-fzsczb8m 33 32 antiserum antiserum NN cord-270326-fzsczb8m 33 33 ( ( -LRB- cord-270326-fzsczb8m 33 34 Dakopatts Dakopatts NNP cord-270326-fzsczb8m 33 35 , , , cord-270326-fzsczb8m 33 36 Denmark Denmark NNP cord-270326-fzsczb8m 33 37 ) ) -RRB- cord-270326-fzsczb8m 33 38 and and CC cord-270326-fzsczb8m 33 39 fluorescein fluorescein NN cord-270326-fzsczb8m 33 40 - - HYPH cord-270326-fzsczb8m 33 41 labeled label VBN cord-270326-fzsczb8m 33 42 goat goat NN cord-270326-fzsczb8m 33 43 anti anti JJ cord-270326-fzsczb8m 33 44 - - JJ cord-270326-fzsczb8m 33 45 rabbit rabbit JJ cord-270326-fzsczb8m 33 46 lgG lgG NNP cord-270326-fzsczb8m 33 47 ( ( -LRB- cord-270326-fzsczb8m 33 48 Miles Miles NNP cord-270326-fzsczb8m 33 49 Laboratories Laboratories NNPS cord-270326-fzsczb8m 33 50 , , , cord-270326-fzsczb8m 33 51 Elkhart Elkhart NNP cord-270326-fzsczb8m 33 52 , , , cord-270326-fzsczb8m 33 53 IN IN NNP cord-270326-fzsczb8m 33 54 , , , cord-270326-fzsczb8m 33 55 U.S.A. U.S.A. NNP cord-270326-fzsczb8m 33 56 ) ) -RRB- cord-270326-fzsczb8m 33 57 . . . cord-270326-fzsczb8m 34 1 The the DT cord-270326-fzsczb8m 34 2 lack lack NN cord-270326-fzsczb8m 34 3 of of IN cord-270326-fzsczb8m 34 4 noticeable noticeable JJ cord-270326-fzsczb8m 34 5 oligodendrocytcs oligodendrocytc NNS cord-270326-fzsczb8m 34 6 and and CC cord-270326-fzsczb8m 34 7 microglial microglial JJ cord-270326-fzsczb8m 34 8 / / SYM cord-270326-fzsczb8m 34 9 macrophage macrophage NN cord-270326-fzsczb8m 34 10 cells cell NNS cord-270326-fzsczb8m 34 11 was be VBD cord-270326-fzsczb8m 34 12 determined determine VBN cord-270326-fzsczb8m 34 13 by by IN cord-270326-fzsczb8m 34 14 using use VBG cord-270326-fzsczb8m 34 15 a a DT cord-270326-fzsczb8m 34 16 guinea guinea NN cord-270326-fzsczb8m 34 17 pig pig NN cord-270326-fzsczb8m 34 18 anti anti JJ cord-270326-fzsczb8m 34 19 - - JJ cord-270326-fzsczb8m 34 20 myelin myelin JJ cord-270326-fzsczb8m 34 21 basic basic JJ cord-270326-fzsczb8m 34 22 protein protein NN cord-270326-fzsczb8m 34 23 ( ( -LRB- cord-270326-fzsczb8m 34 24 MBP MBP NNP cord-270326-fzsczb8m 34 25 ) ) -RRB- cord-270326-fzsczb8m 34 26 antiserum antiserum NN cord-270326-fzsczb8m 34 27 prepared prepare VBN cord-270326-fzsczb8m 34 28 by by IN cord-270326-fzsczb8m 34 29 ourselves -PRON- PRP cord-270326-fzsczb8m 34 30 ( ( -LRB- cord-270326-fzsczb8m 34 31 Rubio Rubio NNP cord-270326-fzsczb8m 34 32 and and CC cord-270326-fzsczb8m 34 33 Cuesta Cuesta NNP cord-270326-fzsczb8m 34 34 , , , cord-270326-fzsczb8m 34 35 1989 1989 CD cord-270326-fzsczb8m 34 36 ) ) -RRB- cord-270326-fzsczb8m 34 37 and and CC cord-270326-fzsczb8m 34 38 a a DT cord-270326-fzsczb8m 34 39 monoclonal monoclonal JJ cord-270326-fzsczb8m 34 40 anti anti NN cord-270326-fzsczb8m 34 41 - - JJ cord-270326-fzsczb8m 34 42 Mac-1 Mac-1 NNP cord-270326-fzsczb8m 34 43 antibody antibody NN cord-270326-fzsczb8m 34 44 ( ( -LRB- cord-270326-fzsczb8m 34 45 Serotec Serotec NNP cord-270326-fzsczb8m 34 46 , , , cord-270326-fzsczb8m 34 47 Oxford Oxford NNP cord-270326-fzsczb8m 34 48 , , , cord-270326-fzsczb8m 34 49 U.K. U.K. NNP cord-270326-fzsczb8m 34 50 ) ) -RRB- cord-270326-fzsczb8m 34 51 . . . cord-270326-fzsczb8m 35 1 Secondary secondary JJ cord-270326-fzsczb8m 35 2 fluorescein fluorescein NN cord-270326-fzsczb8m 35 3 - - HYPH cord-270326-fzsczb8m 35 4 labeled label VBN cord-270326-fzsczb8m 35 5 antibody antibody NN cord-270326-fzsczb8m 35 6 against against IN cord-270326-fzsczb8m 35 7 guinea guinea NN cord-270326-fzsczb8m 35 8 pig pig NN cord-270326-fzsczb8m 35 9 and and CC cord-270326-fzsczb8m 35 10 rat rat NNP cord-270326-fzsczb8m 35 11 IgG IgG NNP cord-270326-fzsczb8m 35 12 were be VBD cord-270326-fzsczb8m 35 13 purchased purchase VBN cord-270326-fzsczb8m 35 14 from from IN cord-270326-fzsczb8m 35 15 Sigma Sigma NNP cord-270326-fzsczb8m 35 16 . . . cord-270326-fzsczb8m 36 1 The the DT cord-270326-fzsczb8m 36 2 rat rat NN cord-270326-fzsczb8m 36 3 astrocytoma astrocytoma NN cord-270326-fzsczb8m 36 4 C-6 C-6 NNP cord-270326-fzsczb8m 36 5 was be VBD cord-270326-fzsczb8m 36 6 obtained obtain VBN cord-270326-fzsczb8m 36 7 from from IN cord-270326-fzsczb8m 36 8 the the DT cord-270326-fzsczb8m 36 9 American American NNP cord-270326-fzsczb8m 36 10 Type Type NNP cord-270326-fzsczb8m 36 11 Culture Culture NNP cord-270326-fzsczb8m 36 12 Collection Collection NNP cord-270326-fzsczb8m 36 13 , , , cord-270326-fzsczb8m 36 14 U.S.A. U.S.A. NNP cord-270326-fzsczb8m 36 15 , , , cord-270326-fzsczb8m 36 16 and and CC cord-270326-fzsczb8m 36 17 cultured culture VBN cord-270326-fzsczb8m 36 18 in in IN cord-270326-fzsczb8m 36 19 DMEM DMEM NNP cord-270326-fzsczb8m 36 20 supplemented supplement VBD cord-270326-fzsczb8m 36 21 with with IN cord-270326-fzsczb8m 36 22 10 10 CD cord-270326-fzsczb8m 36 23 % % NN cord-270326-fzsczb8m 36 24 FCS FCS NNP cord-270326-fzsczb8m 36 25 and and CC cord-270326-fzsczb8m 36 26 gentamicin gentamicin NN cord-270326-fzsczb8m 36 27 . . . cord-270326-fzsczb8m 37 1 Confluent Confluent NNP cord-270326-fzsczb8m 37 2 astrocyte astrocyte IN cord-270326-fzsczb8m 37 3 monolayers monolayer NNS cord-270326-fzsczb8m 37 4 in in IN cord-270326-fzsczb8m 37 5 16 16 CD cord-270326-fzsczb8m 37 6 mm mm NN cord-270326-fzsczb8m 37 7 diameter diameter NN cord-270326-fzsczb8m 37 8 24-well 24-well CD cord-270326-fzsczb8m 37 9 plates plate NNS cord-270326-fzsczb8m 37 10 ( ( -LRB- cord-270326-fzsczb8m 37 11 Costar Costar NNP cord-270326-fzsczb8m 37 12 ) ) -RRB- cord-270326-fzsczb8m 37 13 were be VBD cord-270326-fzsczb8m 37 14 used use VBN cord-270326-fzsczb8m 37 15 in in IN cord-270326-fzsczb8m 37 16 the the DT cord-270326-fzsczb8m 37 17 binding bind VBG cord-270326-fzsczb8m 37 18 experiments experiment NNS cord-270326-fzsczb8m 37 19 . . . cord-270326-fzsczb8m 38 1 Ceils Ceils NNP cord-270326-fzsczb8m 38 2 were be VBD cord-270326-fzsczb8m 38 3 incubated incubate VBN cord-270326-fzsczb8m 38 4 at at IN cord-270326-fzsczb8m 38 5 different different JJ cord-270326-fzsczb8m 38 6 temperatures temperature NNS cord-270326-fzsczb8m 38 7 , , , cord-270326-fzsczb8m 38 8 times time NNS cord-270326-fzsczb8m 38 9 and and CC cord-270326-fzsczb8m 38 10 with with IN cord-270326-fzsczb8m 38 11 or or CC cord-270326-fzsczb8m 38 12 without without IN cord-270326-fzsczb8m 38 13 unlabeled unlabeled JJ cord-270326-fzsczb8m 38 14 IFN-7 IFN-7 NNP cord-270326-fzsczb8m 38 15 , , , cord-270326-fzsczb8m 38 16 as as IN cord-270326-fzsczb8m 38 17 stated state VBN cord-270326-fzsczb8m 38 18 in in IN cord-270326-fzsczb8m 38 19 the the DT cord-270326-fzsczb8m 38 20 text text NN cord-270326-fzsczb8m 38 21 . . . cord-270326-fzsczb8m 39 1 The the DT cord-270326-fzsczb8m 39 2 buffer buffer NN cord-270326-fzsczb8m 39 3 used use VBN cord-270326-fzsczb8m 39 4 was be VBD cord-270326-fzsczb8m 39 5 phosphate phosphate NN cord-270326-fzsczb8m 39 6 - - HYPH cord-270326-fzsczb8m 39 7 buffered buffer VBN cord-270326-fzsczb8m 39 8 saline saline NN cord-270326-fzsczb8m 39 9 ( ( -LRB- cord-270326-fzsczb8m 39 10 PBS PBS NNP cord-270326-fzsczb8m 39 11 ) ) -RRB- cord-270326-fzsczb8m 39 12 containing contain VBG cord-270326-fzsczb8m 39 13 0.1 0.1 CD cord-270326-fzsczb8m 39 14 % % NN cord-270326-fzsczb8m 39 15 bovine bovine JJ cord-270326-fzsczb8m 39 16 serum serum NN cord-270326-fzsczb8m 39 17 albumin albumin NN cord-270326-fzsczb8m 39 18 ( ( -LRB- cord-270326-fzsczb8m 39 19 BSA BSA NNP cord-270326-fzsczb8m 39 20 ) ) -RRB- cord-270326-fzsczb8m 39 21 from from IN cord-270326-fzsczb8m 39 22 Sigma Sigma NNP cord-270326-fzsczb8m 39 23 . . . cord-270326-fzsczb8m 40 1 After after IN cord-270326-fzsczb8m 40 2 washing wash VBG cord-270326-fzsczb8m 40 3 3 3 CD cord-270326-fzsczb8m 40 4 times time NNS cord-270326-fzsczb8m 40 5 with with IN cord-270326-fzsczb8m 40 6 the the DT cord-270326-fzsczb8m 40 7 above above JJ cord-270326-fzsczb8m 40 8 buffer buffer NN cord-270326-fzsczb8m 40 9 , , , cord-270326-fzsczb8m 40 10 cells cell NNS cord-270326-fzsczb8m 40 11 were be VBD cord-270326-fzsczb8m 40 12 detached detach VBN cord-270326-fzsczb8m 40 13 from from IN cord-270326-fzsczb8m 40 14 the the DT cord-270326-fzsczb8m 40 15 plastic plastic NN cord-270326-fzsczb8m 40 16 surface surface NN cord-270326-fzsczb8m 40 17 with with IN cord-270326-fzsczb8m 40 18 1 1 CD cord-270326-fzsczb8m 40 19 N n CD cord-270326-fzsczb8m 40 20 NaOH NaOH NNP cord-270326-fzsczb8m 40 21 at at IN cord-270326-fzsczb8m 40 22 60 60 CD cord-270326-fzsczb8m 40 23 ° ° NN cord-270326-fzsczb8m 40 24 C c NN cord-270326-fzsczb8m 40 25 and and CC cord-270326-fzsczb8m 40 26 counted count VBD cord-270326-fzsczb8m 40 27 in in IN cord-270326-fzsczb8m 40 28 an an DT cord-270326-fzsczb8m 40 29 LKB LKB NNP cord-270326-fzsczb8m 40 30 - - HYPH cord-270326-fzsczb8m 40 31 Wallac Wallac NNP cord-270326-fzsczb8m 40 32 1282 1282 CD cord-270326-fzsczb8m 40 33 Compugamma Compugamma NNP cord-270326-fzsczb8m 40 34 counter counter NN cord-270326-fzsczb8m 40 35 . . . cord-270326-fzsczb8m 41 1 Monolayers monolayer NNS cord-270326-fzsczb8m 41 2 of of IN cord-270326-fzsczb8m 41 3 astrocytes astrocyte NNS cord-270326-fzsczb8m 41 4 in in IN cord-270326-fzsczb8m 41 5 16 16 CD cord-270326-fzsczb8m 41 6 mm mm NN cord-270326-fzsczb8m 41 7 diameter diameter NN cord-270326-fzsczb8m 41 8 24-well 24-well CD cord-270326-fzsczb8m 41 9 plates plate NNS cord-270326-fzsczb8m 41 10 were be VBD cord-270326-fzsczb8m 41 11 incubated incubate VBN cord-270326-fzsczb8m 41 12 with with IN cord-270326-fzsczb8m 41 13 a a DT cord-270326-fzsczb8m 41 14 saturating saturate VBG cord-270326-fzsczb8m 41 15 amount amount NN cord-270326-fzsczb8m 41 16 of of IN cord-270326-fzsczb8m 41 17 unlabeled unlabeled JJ cord-270326-fzsczb8m 41 18 IFN-7 IFN-7 NNP cord-270326-fzsczb8m 41 19 . . . cord-270326-fzsczb8m 42 1 Binding binding NN cord-270326-fzsczb8m 42 2 and and CC cord-270326-fzsczb8m 42 3 endocy endocy NN cord-270326-fzsczb8m 42 4 - - HYPH cord-270326-fzsczb8m 42 5 tosis tosis NN cord-270326-fzsczb8m 42 6 were be VBD cord-270326-fzsczb8m 42 7 allowed allow VBN cord-270326-fzsczb8m 42 8 to to TO cord-270326-fzsczb8m 42 9 take take VB cord-270326-fzsczb8m 42 10 place place NN cord-270326-fzsczb8m 42 11 at at IN cord-270326-fzsczb8m 42 12 4 4 CD cord-270326-fzsczb8m 42 13 ° ° NN cord-270326-fzsczb8m 42 14 C c NN cord-270326-fzsczb8m 42 15 or or CC cord-270326-fzsczb8m 42 16 37 37 CD cord-270326-fzsczb8m 42 17 ° ° NN cord-270326-fzsczb8m 42 18 C c NN cord-270326-fzsczb8m 42 19 for for IN cord-270326-fzsczb8m 42 20 different different JJ cord-270326-fzsczb8m 42 21 periods period NNS cord-270326-fzsczb8m 42 22 of of IN cord-270326-fzsczb8m 42 23 time time NN cord-270326-fzsczb8m 42 24 . . . cord-270326-fzsczb8m 43 1 After after IN cord-270326-fzsczb8m 43 2 two two CD cord-270326-fzsczb8m 43 3 washes wash NNS cord-270326-fzsczb8m 43 4 with with IN cord-270326-fzsczb8m 43 5 PBS PBS NNP cord-270326-fzsczb8m 43 6 - - HYPH cord-270326-fzsczb8m 43 7 BSA BSA NNP cord-270326-fzsczb8m 43 8 to to TO cord-270326-fzsczb8m 43 9 remove remove VB cord-270326-fzsczb8m 43 10 free free JJ cord-270326-fzsczb8m 43 11 IFN-7 IFN-7 NNP cord-270326-fzsczb8m 43 12 , , , cord-270326-fzsczb8m 43 13 cells cell NNS cord-270326-fzsczb8m 43 14 were be VBD cord-270326-fzsczb8m 43 15 fixed fix VBN cord-270326-fzsczb8m 43 16 for for IN cord-270326-fzsczb8m 43 17 30 30 CD cord-270326-fzsczb8m 43 18 rain rain NN cord-270326-fzsczb8m 43 19 at at IN cord-270326-fzsczb8m 43 20 room room NN cord-270326-fzsczb8m 43 21 temperature temperature NN cord-270326-fzsczb8m 43 22 in in IN cord-270326-fzsczb8m 43 23 freshly freshly RB cord-270326-fzsczb8m 43 24 prepared prepare VBN cord-270326-fzsczb8m 43 25 3 3 CD cord-270326-fzsczb8m 43 26 % % NN cord-270326-fzsczb8m 43 27 formaldehyde formaldehyde NN cord-270326-fzsczb8m 43 28 in in IN cord-270326-fzsczb8m 43 29 PBS PBS NNP cord-270326-fzsczb8m 43 30 . . . cord-270326-fzsczb8m 44 1 After after IN cord-270326-fzsczb8m 44 2 cells cell NNS cord-270326-fzsczb8m 44 3 were be VBD cord-270326-fzsczb8m 44 4 washed wash VBN cord-270326-fzsczb8m 44 5 , , , cord-270326-fzsczb8m 44 6 they -PRON- PRP cord-270326-fzsczb8m 44 7 were be VBD cord-270326-fzsczb8m 44 8 incubated incubate VBN cord-270326-fzsczb8m 44 9 with with IN cord-270326-fzsczb8m 44 10 a a DT cord-270326-fzsczb8m 44 11 rabbit rabbit NN cord-270326-fzsczb8m 44 12 anti anti JJ cord-270326-fzsczb8m 44 13 - - JJ cord-270326-fzsczb8m 44 14 murine murine JJ cord-270326-fzsczb8m 44 15 IFN IFN NNP cord-270326-fzsczb8m 44 16 - - HYPH cord-270326-fzsczb8m 44 17 y y NNP cord-270326-fzsczb8m 44 18 antiserum antiserum NN cord-270326-fzsczb8m 44 19 ( ( -LRB- cord-270326-fzsczb8m 44 20 kindly kindly RB cord-270326-fzsczb8m 44 21 provided provide VBN cord-270326-fzsczb8m 44 22 by by IN cord-270326-fzsczb8m 44 23 Dr. Dr. NNP cord-270326-fzsczb8m 44 24 Robert Robert NNP cord-270326-fzsczb8m 44 25 C. C. NNP cord-270326-fzsczb8m 44 26 Curry Curry NNP cord-270326-fzsczb8m 44 27 , , , cord-270326-fzsczb8m 44 28 Bristol Bristol NNP cord-270326-fzsczb8m 44 29 - - HYPH cord-270326-fzsczb8m 44 30 Myers Myers NNP cord-270326-fzsczb8m 44 31 , , , cord-270326-fzsczb8m 44 32 Wallingford Wallingford NNP cord-270326-fzsczb8m 44 33 , , , cord-270326-fzsczb8m 44 34 CT CT NNP cord-270326-fzsczb8m 44 35 , , , cord-270326-fzsczb8m 44 36 U.S.A. U.S.A. NNP cord-270326-fzsczb8m 44 37 ) ) -RRB- cord-270326-fzsczb8m 44 38 diluted dilute VBD cord-270326-fzsczb8m 44 39 1:100 1:100 CD cord-270326-fzsczb8m 44 40 , , , cord-270326-fzsczb8m 44 41 at at IN cord-270326-fzsczb8m 44 42 37 37 CD cord-270326-fzsczb8m 44 43 ° ° NN cord-270326-fzsczb8m 44 44 C c NN cord-270326-fzsczb8m 44 45 for for IN cord-270326-fzsczb8m 44 46 30 30 CD cord-270326-fzsczb8m 44 47 min min NN cord-270326-fzsczb8m 44 48 . . . cord-270326-fzsczb8m 45 1 After after IN cord-270326-fzsczb8m 45 2 two two CD cord-270326-fzsczb8m 45 3 further further JJ cord-270326-fzsczb8m 45 4 washes wash NNS cord-270326-fzsczb8m 45 5 , , , cord-270326-fzsczb8m 45 6 the the DT cord-270326-fzsczb8m 45 7 antibody antibody NN cord-270326-fzsczb8m 45 8 bound bind VBN cord-270326-fzsczb8m 45 9 to to IN cord-270326-fzsczb8m 45 10 IFN IFN NNP cord-270326-fzsczb8m 45 11 - - HYPH cord-270326-fzsczb8m 45 12 y y NNP cord-270326-fzsczb8m 45 13 available available JJ cord-270326-fzsczb8m 45 14 on on IN cord-270326-fzsczb8m 45 15 the the DT cord-270326-fzsczb8m 45 16 astrocytes astrocyte NNS cord-270326-fzsczb8m 45 17 surface surface NN cord-270326-fzsczb8m 45 18 was be VBD cord-270326-fzsczb8m 45 19 detected detect VBN cord-270326-fzsczb8m 45 20 by by IN cord-270326-fzsczb8m 45 21 incubation incubation NN cord-270326-fzsczb8m 45 22 with with IN cord-270326-fzsczb8m 45 23 radioiodinated radioiodinated JJ cord-270326-fzsczb8m 45 24 staphylococcal staphylococcal JJ cord-270326-fzsczb8m 45 25 protein protein NN cord-270326-fzsczb8m 45 26 A A NNP cord-270326-fzsczb8m 45 27 ( ( -LRB- cord-270326-fzsczb8m 45 28 100,000 100,000 CD cord-270326-fzsczb8m 45 29 cpm cpm NN cord-270326-fzsczb8m 45 30 , , , cord-270326-fzsczb8m 45 31 30 30 CD cord-270326-fzsczb8m 45 32 min min NN cord-270326-fzsczb8m 45 33 , , , cord-270326-fzsczb8m 45 34 37 37 CD cord-270326-fzsczb8m 45 35 ° ° NN cord-270326-fzsczb8m 45 36 C C NNP cord-270326-fzsczb8m 45 37 ) ) -RRB- cord-270326-fzsczb8m 45 38 . . . cord-270326-fzsczb8m 46 1 After after IN cord-270326-fzsczb8m 46 2 washing washing NN cord-270326-fzsczb8m 46 3 , , , cord-270326-fzsczb8m 46 4 cells cell NNS cord-270326-fzsczb8m 46 5 were be VBD cord-270326-fzsczb8m 46 6 detached detach VBN cord-270326-fzsczb8m 46 7 with with IN cord-270326-fzsczb8m 46 8 1 1 CD cord-270326-fzsczb8m 46 9 N n CD cord-270326-fzsczb8m 46 10 NaOH NaOH NNP cord-270326-fzsczb8m 46 11 and and CC cord-270326-fzsczb8m 46 12 counted count VBD cord-270326-fzsczb8m 46 13 . . . cord-270326-fzsczb8m 47 1 To to TO cord-270326-fzsczb8m 47 2 assess assess VB cord-270326-fzsczb8m 47 3 total total JJ cord-270326-fzsczb8m 47 4 internalized internalized JJ cord-270326-fzsczb8m 47 5 ligand ligand NN cord-270326-fzsczb8m 47 6 , , , cord-270326-fzsczb8m 47 7 we -PRON- PRP cord-270326-fzsczb8m 47 8 removed remove VBD cord-270326-fzsczb8m 47 9 surface surface NN cord-270326-fzsczb8m 47 10 - - HYPH cord-270326-fzsczb8m 47 11 bound bind VBN cord-270326-fzsczb8m 47 12 125I 125I NNP cord-270326-fzsczb8m 47 13 - - HYPH cord-270326-fzsczb8m 47 14 IFN IFN NNP cord-270326-fzsczb8m 47 15 - - HYPH cord-270326-fzsczb8m 47 16 y y NNP cord-270326-fzsczb8m 47 17 from from IN cord-270326-fzsczb8m 47 18 twice twice RB cord-270326-fzsczb8m 47 19 - - HYPH cord-270326-fzsczb8m 47 20 washed wash VBN cord-270326-fzsczb8m 47 21 cells cell NNS cord-270326-fzsczb8m 47 22 with with IN cord-270326-fzsczb8m 47 23 2 2 CD cord-270326-fzsczb8m 47 24 ml ml NNS cord-270326-fzsczb8m 47 25 of of IN cord-270326-fzsczb8m 47 26 0.15 0.15 CD cord-270326-fzsczb8m 47 27 M M NNP cord-270326-fzsczb8m 47 28 NaC1 NaC1 NNP cord-270326-fzsczb8m 48 1 -50 -50 NN cord-270326-fzsczb8m 48 2 mM mm CD cord-270326-fzsczb8m 48 3 glycine glycine NN cord-270326-fzsczb8m 48 4 , , , cord-270326-fzsczb8m 48 5 pH pH NNP cord-270326-fzsczb8m 48 6 3.0 3.0 CD cord-270326-fzsczb8m 48 7 at at IN cord-270326-fzsczb8m 48 8 4 4 CD cord-270326-fzsczb8m 48 9 ° ° NN cord-270326-fzsczb8m 48 10 C c NN cord-270326-fzsczb8m 48 11 for for IN cord-270326-fzsczb8m 48 12 2 2 CD cord-270326-fzsczb8m 48 13 min min NN cord-270326-fzsczb8m 48 14 ( ( -LRB- cord-270326-fzsczb8m 48 15 Costlow Costlow NNP cord-270326-fzsczb8m 48 16 and and CC cord-270326-fzsczb8m 48 17 Hample Hample NNP cord-270326-fzsczb8m 48 18 , , , cord-270326-fzsczb8m 48 19 1982 1982 CD cord-270326-fzsczb8m 48 20 ) ) -RRB- cord-270326-fzsczb8m 48 21 . . . cord-270326-fzsczb8m 49 1 After after IN cord-270326-fzsczb8m 49 2 two two CD cord-270326-fzsczb8m 49 3 more more JJR cord-270326-fzsczb8m 49 4 washes wash NNS cord-270326-fzsczb8m 49 5 with with IN cord-270326-fzsczb8m 49 6 PBS PBS NNP cord-270326-fzsczb8m 49 7 , , , cord-270326-fzsczb8m 49 8 cells cell NNS cord-270326-fzsczb8m 49 9 were be VBD cord-270326-fzsczb8m 49 10 detached detach VBN cord-270326-fzsczb8m 49 11 and and CC cord-270326-fzsczb8m 49 12 counted count VBN cord-270326-fzsczb8m 49 13 in in IN cord-270326-fzsczb8m 49 14 the the DT cord-270326-fzsczb8m 49 15 gamma gamma NN cord-270326-fzsczb8m 49 16 counter counter NN cord-270326-fzsczb8m 49 17 . . . cord-270326-fzsczb8m 50 1 Scatchard Scatchard NNP cord-270326-fzsczb8m 50 2 and and CC cord-270326-fzsczb8m 50 3 Hill Hill NNP cord-270326-fzsczb8m 50 4 plot plot NN cord-270326-fzsczb8m 50 5 analysis analysis NN cord-270326-fzsczb8m 50 6 of of IN cord-270326-fzsczb8m 50 7 the the DT cord-270326-fzsczb8m 50 8 data datum NNS cord-270326-fzsczb8m 50 9 from from IN cord-270326-fzsczb8m 50 10 binding bind VBG cord-270326-fzsczb8m 50 11 assays assay NNS cord-270326-fzsczb8m 50 12 was be VBD cord-270326-fzsczb8m 50 13 performed perform VBN cord-270326-fzsczb8m 50 14 employing employ VBG cord-270326-fzsczb8m 50 15 the the DT cord-270326-fzsczb8m 50 16 LIGAND LIGAND NNP cord-270326-fzsczb8m 50 17 program program NN cord-270326-fzsczb8m 50 18 ( ( -LRB- cord-270326-fzsczb8m 50 19 Munson Munson NNP cord-270326-fzsczb8m 50 20 and and CC cord-270326-fzsczb8m 50 21 Rodbard Rodbard NNP cord-270326-fzsczb8m 50 22 , , , cord-270326-fzsczb8m 50 23 1980 1980 CD cord-270326-fzsczb8m 50 24 ) ) -RRB- cord-270326-fzsczb8m 50 25 . . . cord-270326-fzsczb8m 51 1 The the DT cord-270326-fzsczb8m 51 2 data datum NNS cord-270326-fzsczb8m 51 3 shown show VBN cord-270326-fzsczb8m 51 4 represent represent VBP cord-270326-fzsczb8m 51 5 mean mean JJ cord-270326-fzsczb8m 51 6 results result NNS cord-270326-fzsczb8m 51 7 of of IN cord-270326-fzsczb8m 51 8 three three CD cord-270326-fzsczb8m 51 9 reproducible reproducible JJ cord-270326-fzsczb8m 51 10 experiments experiment NNS cord-270326-fzsczb8m 51 11 + + CC cord-270326-fzsczb8m 51 12 standard standard JJ cord-270326-fzsczb8m 51 13 deviations deviation NNS cord-270326-fzsczb8m 51 14 . . . cord-270326-fzsczb8m 52 1 Fig Fig NNP cord-270326-fzsczb8m 52 2 . . NNP cord-270326-fzsczb8m 52 3 1 1 CD cord-270326-fzsczb8m 52 4 shows show VBZ cord-270326-fzsczb8m 52 5 the the DT cord-270326-fzsczb8m 52 6 kinetics kinetic NNS cord-270326-fzsczb8m 52 7 of of IN cord-270326-fzsczb8m 52 8 125I 125I NNP cord-270326-fzsczb8m 52 9 - - HYPH cord-270326-fzsczb8m 52 10 labeled label VBN cord-270326-fzsczb8m 52 11 IFN-7 IFN-7 NNP cord-270326-fzsczb8m 52 12 binding bind VBG cord-270326-fzsczb8m 52 13 to to IN cord-270326-fzsczb8m 52 14 pure pure JJ cord-270326-fzsczb8m 52 15 astrocyte astrocyte NNP cord-270326-fzsczb8m 52 16 cultures culture NNS cord-270326-fzsczb8m 52 17 . . . cord-270326-fzsczb8m 53 1 Care care NN cord-270326-fzsczb8m 53 2 has have VBZ cord-270326-fzsczb8m 53 3 been be VBN cord-270326-fzsczb8m 53 4 exercised exercise VBN cord-270326-fzsczb8m 53 5 to to TO cord-270326-fzsczb8m 53 6 use use VB cord-270326-fzsczb8m 53 7 only only JJ cord-270326-fzsczb8m 53 8 cultures culture NNS cord-270326-fzsczb8m 53 9 containing contain VBG cord-270326-fzsczb8m 53 10 around around IN cord-270326-fzsczb8m 53 11 99 99 CD cord-270326-fzsczb8m 53 12 % % NN cord-270326-fzsczb8m 53 13 antrocytes antrocyte NNS cord-270326-fzsczb8m 53 14 , , , cord-270326-fzsczb8m 53 15 free free JJ cord-270326-fzsczb8m 53 16 of of IN cord-270326-fzsczb8m 53 17 oligodendrocytes oligodendrocyte NNS cord-270326-fzsczb8m 53 18 or or CC cord-270326-fzsczb8m 53 19 microglial microglial JJ cord-270326-fzsczb8m 53 20 / / SYM cord-270326-fzsczb8m 53 21 macrophage macrophage NN cord-270326-fzsczb8m 53 22 cells cell NNS cord-270326-fzsczb8m 53 23 according accord VBG cord-270326-fzsczb8m 53 24 to to IN cord-270326-fzsczb8m 53 25 criteria criterion NNS cord-270326-fzsczb8m 53 26 explained explain VBN cord-270326-fzsczb8m 53 27 under under IN cord-270326-fzsczb8m 53 28 Materials material NNS cord-270326-fzsczb8m 53 29 and and CC cord-270326-fzsczb8m 53 30 methods method NNS cord-270326-fzsczb8m 53 31 . . . cord-270326-fzsczb8m 54 1 Saturation saturation NN cord-270326-fzsczb8m 54 2 of of IN cord-270326-fzsczb8m 54 3 the the DT cord-270326-fzsczb8m 54 4 binding bind VBG cord-270326-fzsczb8m 54 5 capacity capacity NN cord-270326-fzsczb8m 54 6 of of IN cord-270326-fzsczb8m 54 7 1 1 CD cord-270326-fzsczb8m 54 8 × × CD cord-270326-fzsczb8m 54 9 105 105 CD cord-270326-fzsczb8m 54 10 astrocytic astrocytic JJ cord-270326-fzsczb8m 54 11 cells cell NNS cord-270326-fzsczb8m 54 12 takes take VBZ cord-270326-fzsczb8m 54 13 place place NN cord-270326-fzsczb8m 54 14 in in IN cord-270326-fzsczb8m 54 15 60 60 CD cord-270326-fzsczb8m 54 16 min min NN cord-270326-fzsczb8m 54 17 at at IN cord-270326-fzsczb8m 54 18 37 37 CD cord-270326-fzsczb8m 54 19 ° ° NN cord-270326-fzsczb8m 54 20 C. C. NNP cord-270326-fzsczb8m 54 21 At at IN cord-270326-fzsczb8m 54 22 4 4 CD cord-270326-fzsczb8m 54 23 ° ° NN cord-270326-fzsczb8m 54 24 C c NN cord-270326-fzsczb8m 54 25 , , , cord-270326-fzsczb8m 54 26 a a DT cord-270326-fzsczb8m 54 27 120 120 CD cord-270326-fzsczb8m 54 28 min min NN cord-270326-fzsczb8m 54 29 incubation incubation NN cord-270326-fzsczb8m 54 30 period period NN cord-270326-fzsczb8m 54 31 is be VBZ cord-270326-fzsczb8m 54 32 required require VBN cord-270326-fzsczb8m 54 33 to to TO cord-270326-fzsczb8m 54 34 reach reach VB cord-270326-fzsczb8m 54 35 maximal maximal JJ cord-270326-fzsczb8m 54 36 binding binding NN cord-270326-fzsczb8m 54 37 . . . cord-270326-fzsczb8m 55 1 Maximal maximal JJ cord-270326-fzsczb8m 55 2 binding binding NN cord-270326-fzsczb8m 55 3 is be VBZ cord-270326-fzsczb8m 55 4 found find VBN cord-270326-fzsczb8m 55 5 to to TO cord-270326-fzsczb8m 55 6 be be VB cord-270326-fzsczb8m 55 7 around around RB cord-270326-fzsczb8m 55 8 5 5 CD cord-270326-fzsczb8m 55 9 % % NN cord-270326-fzsczb8m 55 10 of of IN cord-270326-fzsczb8m 55 11 the the DT cord-270326-fzsczb8m 55 12 input input NN cord-270326-fzsczb8m 55 13 , , , cord-270326-fzsczb8m 55 14 which which WDT cord-270326-fzsczb8m 55 15 is be VBZ cord-270326-fzsczb8m 55 16 kept keep VBN cord-270326-fzsczb8m 55 17 constant constant JJ cord-270326-fzsczb8m 55 18 in in IN cord-270326-fzsczb8m 55 19 100,000 100,000 CD cord-270326-fzsczb8m 55 20 cpm cpm NN cord-270326-fzsczb8m 55 21 per per FW cord-270326-fzsczb8m 55 22 well well RB cord-270326-fzsczb8m 55 23 in in IN cord-270326-fzsczb8m 55 24 this this DT cord-270326-fzsczb8m 55 25 work work NN cord-270326-fzsczb8m 55 26 . . . cord-270326-fzsczb8m 56 1 To to TO cord-270326-fzsczb8m 56 2 demonstrate demonstrate VB cord-270326-fzsczb8m 56 3 that that IN cord-270326-fzsczb8m 56 4 the the DT cord-270326-fzsczb8m 56 5 binding binding NN cord-270326-fzsczb8m 56 6 shown show VBN cord-270326-fzsczb8m 56 7 in in IN cord-270326-fzsczb8m 56 8 Fig Fig NNP cord-270326-fzsczb8m 56 9 . . . cord-270326-fzsczb8m 56 10 _SP cord-270326-fzsczb8m 57 1 1 1 CD cord-270326-fzsczb8m 57 2 is be VBZ cord-270326-fzsczb8m 57 3 specific specific JJ cord-270326-fzsczb8m 57 4 , , , cord-270326-fzsczb8m 57 5 labeled label VBN cord-270326-fzsczb8m 57 6 IFN-7 IFN-7 NNP cord-270326-fzsczb8m 57 7 was be VBD cord-270326-fzsczb8m 57 8 allowed allow VBN cord-270326-fzsczb8m 57 9 to to TO cord-270326-fzsczb8m 57 10 bind bind VB cord-270326-fzsczb8m 57 11 cells cell NNS cord-270326-fzsczb8m 57 12 in in IN cord-270326-fzsczb8m 57 13 the the DT cord-270326-fzsczb8m 57 14 presence presence NN cord-270326-fzsczb8m 57 15 of of IN cord-270326-fzsczb8m 57 16 a a DT cord-270326-fzsczb8m 57 17 100-fold 100-fold CD cord-270326-fzsczb8m 57 18 excess excess NN cord-270326-fzsczb8m 57 19 of of IN cord-270326-fzsczb8m 57 20 the the DT cord-270326-fzsczb8m 57 21 unlabeled unlabeled JJ cord-270326-fzsczb8m 57 22 recombinant recombinant JJ cord-270326-fzsczb8m 57 23 molecule molecule NN cord-270326-fzsczb8m 57 24 . . . cord-270326-fzsczb8m 58 1 The the DT cord-270326-fzsczb8m 58 2 saturation saturation NN cord-270326-fzsczb8m 58 3 of of IN cord-270326-fzsczb8m 58 4 specific specific JJ cord-270326-fzsczb8m 58 5 receptor receptor NN cord-270326-fzsczb8m 58 6 sites site NNS cord-270326-fzsczb8m 58 7 is be VBZ cord-270326-fzsczb8m 58 8 depicted depict VBN cord-270326-fzsczb8m 58 9 in in IN cord-270326-fzsczb8m 58 10 Fig Fig NNP cord-270326-fzsczb8m 58 11 . . NNP cord-270326-fzsczb8m 58 12 2 2 CD cord-270326-fzsczb8m 58 13 , , , cord-270326-fzsczb8m 58 14 and and CC cord-270326-fzsczb8m 58 15 shows show VBZ cord-270326-fzsczb8m 58 16 that that IN cord-270326-fzsczb8m 58 17 the the DT cord-270326-fzsczb8m 58 18 binding binding NN cord-270326-fzsczb8m 58 19 of of IN cord-270326-fzsczb8m 58 20 IFN IFN NNP cord-270326-fzsczb8m 58 21 - - HYPH cord-270326-fzsczb8m 58 22 T T NNP cord-270326-fzsczb8m 58 23 to to IN cord-270326-fzsczb8m 58 24 cells cell NNS cord-270326-fzsczb8m 58 25 is be VBZ cord-270326-fzsczb8m 58 26 inhibited inhibit VBN cord-270326-fzsczb8m 58 27 by by IN cord-270326-fzsczb8m 58 28 the the DT cord-270326-fzsczb8m 58 29 presence presence NN cord-270326-fzsczb8m 58 30 of of IN cord-270326-fzsczb8m 58 31 a a DT cord-270326-fzsczb8m 58 32 constant constant JJ cord-270326-fzsczb8m 58 33 amount amount NN cord-270326-fzsczb8m 58 34 of of IN cord-270326-fzsczb8m 58 35 unlabeled unlabeled JJ cord-270326-fzsczb8m 58 36 ligand ligand NN cord-270326-fzsczb8m 58 37 . . . cord-270326-fzsczb8m 59 1 If if IN cord-270326-fzsczb8m 59 2 specific specific JJ cord-270326-fzsczb8m 59 3 binding binding NN cord-270326-fzsczb8m 59 4 is be VBZ cord-270326-fzsczb8m 59 5 defined define VBN cord-270326-fzsczb8m 59 6 as as IN cord-270326-fzsczb8m 59 7 that that DT cord-270326-fzsczb8m 59 8 binding binding NN cord-270326-fzsczb8m 59 9 which which WDT cord-270326-fzsczb8m 59 10 is be VBZ cord-270326-fzsczb8m 59 11 inhibited inhibit VBN cord-270326-fzsczb8m 59 12 in in IN cord-270326-fzsczb8m 59 13 the the DT cord-270326-fzsczb8m 59 14 presence presence NN cord-270326-fzsczb8m 59 15 of of IN cord-270326-fzsczb8m 59 16 an an DT cord-270326-fzsczb8m 59 17 excess excess NN cord-270326-fzsczb8m 59 18 of of IN cord-270326-fzsczb8m 59 19 ' ' `` cord-270326-fzsczb8m 59 20 cold cold JJ cord-270326-fzsczb8m 59 21 ' ' '' cord-270326-fzsczb8m 59 22 IFN IFN NNP cord-270326-fzsczb8m 59 23 - - HYPH cord-270326-fzsczb8m 59 24 y y NNP cord-270326-fzsczb8m 59 25 , , , cord-270326-fzsczb8m 59 26 it -PRON- PRP cord-270326-fzsczb8m 59 27 is be VBZ cord-270326-fzsczb8m 59 28 approximately approximately RB cord-270326-fzsczb8m 59 29 65 65 CD cord-270326-fzsczb8m 59 30 % % NN cord-270326-fzsczb8m 59 31 in in IN cord-270326-fzsczb8m 59 32 this this DT cord-270326-fzsczb8m 59 33 system system NN cord-270326-fzsczb8m 59 34 . . . cord-270326-fzsczb8m 60 1 When when WRB cord-270326-fzsczb8m 60 2 these these DT cord-270326-fzsczb8m 60 3 data datum NNS cord-270326-fzsczb8m 60 4 are be VBP cord-270326-fzsczb8m 60 5 analyzed analyze VBN cord-270326-fzsczb8m 60 6 by by IN cord-270326-fzsczb8m 60 7 the the DT cord-270326-fzsczb8m 60 8 LI LI NNP cord-270326-fzsczb8m 60 9 - - HYPH cord-270326-fzsczb8m 60 10 GAND GAND NNP cord-270326-fzsczb8m 60 11 program program NN cord-270326-fzsczb8m 60 12 , , , cord-270326-fzsczb8m 60 13 a a DT cord-270326-fzsczb8m 60 14 Scatchard Scatchard NNP cord-270326-fzsczb8m 60 15 plot plot NN cord-270326-fzsczb8m 60 16 ( ( -LRB- cord-270326-fzsczb8m 60 17 Scatchard Scatchard NNP cord-270326-fzsczb8m 60 18 , , , cord-270326-fzsczb8m 60 19 1949 1949 CD cord-270326-fzsczb8m 60 20 ) ) -RRB- cord-270326-fzsczb8m 60 21 is be VBZ cord-270326-fzsczb8m 60 22 obtained obtain VBN cord-270326-fzsczb8m 60 23 showing show VBG cord-270326-fzsczb8m 60 24 a a DT cord-270326-fzsczb8m 60 25 K K NNP cord-270326-fzsczb8m 60 26 d d NN cord-270326-fzsczb8m 60 27 of of IN cord-270326-fzsczb8m 60 28 1.64 1.64 CD cord-270326-fzsczb8m 60 29 × × NN cord-270326-fzsczb8m 60 30 10 10 CD cord-270326-fzsczb8m 60 31 -9 -9 NNP cord-270326-fzsczb8m 60 32 M M NNP cord-270326-fzsczb8m 60 33 and and CC cord-270326-fzsczb8m 60 34 a a DT cord-270326-fzsczb8m 60 35 Bma Bma NNP cord-270326-fzsczb8m 60 36 x x NN cord-270326-fzsczb8m 60 37 of of IN cord-270326-fzsczb8m 60 38 3.78 3.78 CD cord-270326-fzsczb8m 60 39 × × NN cord-270326-fzsczb8m 60 40 10 10 CD cord-270326-fzsczb8m 61 1 -11 -11 IN cord-270326-fzsczb8m 61 2 M M NNP cord-270326-fzsczb8m 61 3 ( ( -LRB- cord-270326-fzsczb8m 61 4 Fig Fig NNP cord-270326-fzsczb8m 61 5 . . NNP cord-270326-fzsczb8m 61 6 2 2 CD cord-270326-fzsczb8m 61 7 , , , cord-270326-fzsczb8m 61 8 inset inset NN cord-270326-fzsczb8m 61 9 ) ) -RRB- cord-270326-fzsczb8m 61 10 . . . cord-270326-fzsczb8m 62 1 The the DT cord-270326-fzsczb8m 62 2 number number NN cord-270326-fzsczb8m 62 3 of of IN cord-270326-fzsczb8m 62 4 receptor receptor NN cord-270326-fzsczb8m 62 5 molecules molecule NNS cord-270326-fzsczb8m 62 6 per per IN cord-270326-fzsczb8m 62 7 cell cell NN cord-270326-fzsczb8m 62 8 is be VBZ cord-270326-fzsczb8m 62 9 calculated calculate VBN cord-270326-fzsczb8m 62 10 to to TO cord-270326-fzsczb8m 62 11 be be VB cord-270326-fzsczb8m 62 12 11,100 11,100 CD cord-270326-fzsczb8m 62 13 . . . cord-270326-fzsczb8m 63 1 The the DT cord-270326-fzsczb8m 63 2 Hill Hill NNP cord-270326-fzsczb8m 63 3 plot plot NN cord-270326-fzsczb8m 63 4 shows show VBZ cord-270326-fzsczb8m 63 5 that that IN cord-270326-fzsczb8m 63 6 the the DT cord-270326-fzsczb8m 63 7 astrocyte astrocyte NNP cord-270326-fzsczb8m 63 8 surface surface NN cord-270326-fzsczb8m 63 9 receptor receptor NN cord-270326-fzsczb8m 63 10 binds bind VBZ cord-270326-fzsczb8m 63 11 IFN IFN NNP cord-270326-fzsczb8m 63 12 - - HYPH cord-270326-fzsczb8m 63 13 y y NNP cord-270326-fzsczb8m 63 14 in in IN cord-270326-fzsczb8m 63 15 a a DT cord-270326-fzsczb8m 63 16 homogenous homogenous JJ cord-270326-fzsczb8m 63 17 , , , cord-270326-fzsczb8m 63 18 noncooperative noncooperative JJ cord-270326-fzsczb8m 63 19 manner manner NN cord-270326-fzsczb8m 63 20 . . . cord-270326-fzsczb8m 64 1 Halfsaturation Halfsaturation NNP cord-270326-fzsczb8m 64 2 is be VBZ cord-270326-fzsczb8m 64 3 achieved achieve VBN cord-270326-fzsczb8m 64 4 at at IN cord-270326-fzsczb8m 64 5 a a DT cord-270326-fzsczb8m 64 6 concentration concentration NN cord-270326-fzsczb8m 64 7 of of IN cord-270326-fzsczb8m 64 8 free free JJ cord-270326-fzsczb8m 64 9 ligand ligand NN cord-270326-fzsczb8m 64 10 of of IN cord-270326-fzsczb8m 64 11 about about RB cord-270326-fzsczb8m 64 12 200 200 CD cord-270326-fzsczb8m 64 13 pmol pmol NN cord-270326-fzsczb8m 64 14 ( ( -LRB- cord-270326-fzsczb8m 64 15 0.2 0.2 CD cord-270326-fzsczb8m 64 16 × × NN cord-270326-fzsczb8m 64 17 10 10 CD cord-270326-fzsczb8m 64 18 -9 -9 NNP cord-270326-fzsczb8m 64 19 M M NNP cord-270326-fzsczb8m 64 20 ) ) -RRB- cord-270326-fzsczb8m 64 21 that that WDT cord-270326-fzsczb8m 64 22 is be VBZ cord-270326-fzsczb8m 64 23 in in IN cord-270326-fzsczb8m 64 24 the the DT cord-270326-fzsczb8m 64 25 range range NN cord-270326-fzsczb8m 64 26 of of IN cord-270326-fzsczb8m 64 27 the the DT cord-270326-fzsczb8m 64 28 K K NNP cord-270326-fzsczb8m 64 29 d d NNP cord-270326-fzsczb8m 64 30 reported report VBD cord-270326-fzsczb8m 64 31 . . . cord-270326-fzsczb8m 65 1 Different different JJ cord-270326-fzsczb8m 65 2 numbers number NNS cord-270326-fzsczb8m 65 3 of of IN cord-270326-fzsczb8m 65 4 mouse mouse NN cord-270326-fzsczb8m 65 5 astrocytes astrocyte NNS cord-270326-fzsczb8m 65 6 were be VBD cord-270326-fzsczb8m 65 7 allowed allow VBN cord-270326-fzsczb8m 65 8 to to TO cord-270326-fzsczb8m 65 9 attach attach VB cord-270326-fzsczb8m 65 10 to to IN cord-270326-fzsczb8m 65 11 16 16 CD cord-270326-fzsczb8m 65 12 mm mm NN cord-270326-fzsczb8m 65 13 diameter diameter NN cord-270326-fzsczb8m 65 14 wells well NNS cord-270326-fzsczb8m 65 15 and and CC cord-270326-fzsczb8m 65 16 incubated incubate VBN cord-270326-fzsczb8m 65 17 with with IN cord-270326-fzsczb8m 65 18 100,000 100,000 CD cord-270326-fzsczb8m 65 19 cpm cpm NN cord-270326-fzsczb8m 65 20 of of IN cord-270326-fzsczb8m 65 21 IFN IFN NNP cord-270326-fzsczb8m 65 22 - - HYPH cord-270326-fzsczb8m 65 23 y y NNP cord-270326-fzsczb8m 65 24 . . . cord-270326-fzsczb8m 66 1 As as IN cord-270326-fzsczb8m 66 2 shown show VBN cord-270326-fzsczb8m 66 3 in in IN cord-270326-fzsczb8m 66 4 Fig Fig NNP cord-270326-fzsczb8m 66 5 . . . cord-270326-fzsczb8m 67 1 3 3 LS cord-270326-fzsczb8m 67 2 , , , cord-270326-fzsczb8m 67 3 the the DT cord-270326-fzsczb8m 67 4 binding binding NN cord-270326-fzsczb8m 67 5 reaches reach VBZ cord-270326-fzsczb8m 67 6 a a DT cord-270326-fzsczb8m 67 7 plateau plateau NN cord-270326-fzsczb8m 67 8 when when WRB cord-270326-fzsczb8m 67 9 5 5 CD cord-270326-fzsczb8m 67 10 - - SYM cord-270326-fzsczb8m 67 11 10 10 CD cord-270326-fzsczb8m 67 12 X x SYM cord-270326-fzsczb8m 67 13 10 10 CD cord-270326-fzsczb8m 67 14 4 4 CD cord-270326-fzsczb8m 67 15 cells cell NNS cord-270326-fzsczb8m 67 16 are be VBP cord-270326-fzsczb8m 67 17 plated plate VBN cord-270326-fzsczb8m 67 18 per per IN cord-270326-fzsczb8m 67 19 well well RB cord-270326-fzsczb8m 67 20 . . . cord-270326-fzsczb8m 68 1 This this DT cord-270326-fzsczb8m 68 2 indicates indicate VBZ cord-270326-fzsczb8m 68 3 that that IN cord-270326-fzsczb8m 68 4 the the DT cord-270326-fzsczb8m 68 5 binding binding NN cord-270326-fzsczb8m 68 6 is be VBZ cord-270326-fzsczb8m 68 7 receptor receptor NN cord-270326-fzsczb8m 68 8 - - HYPH cord-270326-fzsczb8m 68 9 specific specific JJ cord-270326-fzsczb8m 68 10 and and CC cord-270326-fzsczb8m 68 11 cell cell NN cord-270326-fzsczb8m 68 12 number number NN cord-270326-fzsczb8m 68 13 - - HYPH cord-270326-fzsczb8m 68 14 dependent dependent JJ cord-270326-fzsczb8m 68 15 . . . cord-270326-fzsczb8m 69 1 As as IN cord-270326-fzsczb8m 69 2 described describe VBN cord-270326-fzsczb8m 69 3 by by IN cord-270326-fzsczb8m 69 4 other other JJ cord-270326-fzsczb8m 69 5 authors author NNS cord-270326-fzsczb8m 69 6 in in IN cord-270326-fzsczb8m 69 7 studies study NNS cord-270326-fzsczb8m 69 8 about about IN cord-270326-fzsczb8m 69 9 IFN IFN NNP cord-270326-fzsczb8m 69 10 - - HYPH cord-270326-fzsczb8m 69 11 y y NNP cord-270326-fzsczb8m 69 12 receptor receptor NN cord-270326-fzsczb8m 69 13 in in IN cord-270326-fzsczb8m 69 14 macrophages macrophage NNS cord-270326-fzsczb8m 69 15 , , , cord-270326-fzsczb8m 69 16 rat rat NN cord-270326-fzsczb8m 69 17 cells cell NNS cord-270326-fzsczb8m 69 18 do do VBP cord-270326-fzsczb8m 69 19 not not RB cord-270326-fzsczb8m 69 20 recognize recognize VB cord-270326-fzsczb8m 69 21 murine murine JJ cord-270326-fzsczb8m 69 22 IFN IFN NNP cord-270326-fzsczb8m 69 23 - - HYPH cord-270326-fzsczb8m 69 24 y y NNP cord-270326-fzsczb8m 70 1 ( ( -LRB- cord-270326-fzsczb8m 70 2 Celada Celada NNP cord-270326-fzsczb8m 70 3 et et NNP cord-270326-fzsczb8m 70 4 al al NNP cord-270326-fzsczb8m 70 5 . . NNP cord-270326-fzsczb8m 70 6 , , , cord-270326-fzsczb8m 70 7 1984 1984 CD cord-270326-fzsczb8m 70 8 ) ) -RRB- cord-270326-fzsczb8m 70 9 . . . cord-270326-fzsczb8m 71 1 Wistar Wistar NNP cord-270326-fzsczb8m 71 2 rat rat NN cord-270326-fzsczb8m 71 3 astrocytes astrocyte NNS cord-270326-fzsczb8m 71 4 do do VBP cord-270326-fzsczb8m 71 5 not not RB cord-270326-fzsczb8m 71 6 bind bind VB cord-270326-fzsczb8m 71 7 any any DT cord-270326-fzsczb8m 71 8 ligand ligand NN cord-270326-fzsczb8m 71 9 during during IN cord-270326-fzsczb8m 71 10 the the DT cord-270326-fzsczb8m 71 11 time time NN cord-270326-fzsczb8m 71 12 of of IN cord-270326-fzsczb8m 71 13 incubation incubation NN cord-270326-fzsczb8m 71 14 used use VBN cord-270326-fzsczb8m 71 15 by by IN cord-270326-fzsczb8m 71 16 us -PRON- PRP cord-270326-fzsczb8m 71 17 ( ( -LRB- cord-270326-fzsczb8m 71 18 Fig Fig NNP cord-270326-fzsczb8m 71 19 . . . cord-270326-fzsczb8m 71 20 3 3 CD cord-270326-fzsczb8m 71 21 ) ) -RRB- cord-270326-fzsczb8m 71 22 . . . cord-270326-fzsczb8m 72 1 C-6 C-6 NNP cord-270326-fzsczb8m 72 2 cells cell NNS cord-270326-fzsczb8m 72 3 , , , cord-270326-fzsczb8m 72 4 an an DT cord-270326-fzsczb8m 72 5 astrocytoma astrocytoma NN cord-270326-fzsczb8m 72 6 cell cell NN cord-270326-fzsczb8m 72 7 line line NN cord-270326-fzsczb8m 72 8 of of IN cord-270326-fzsczb8m 72 9 rat rat NN cord-270326-fzsczb8m 72 10 origin origin NN cord-270326-fzsczb8m 72 11 , , , cord-270326-fzsczb8m 72 12 do do VBP cord-270326-fzsczb8m 72 13 not not RB cord-270326-fzsczb8m 72 14 either either RB cord-270326-fzsczb8m 72 15 show show VB cord-270326-fzsczb8m 72 16 any any DT cord-270326-fzsczb8m 72 17 binding binding NN cord-270326-fzsczb8m 72 18 ( ( -LRB- cord-270326-fzsczb8m 72 19 not not RB cord-270326-fzsczb8m 72 20 shown show VBN cord-270326-fzsczb8m 72 21 ) ) -RRB- cord-270326-fzsczb8m 72 22 . . . cord-270326-fzsczb8m 73 1 These these DT cord-270326-fzsczb8m 73 2 data datum NNS cord-270326-fzsczb8m 73 3 confirm confirm VBP cord-270326-fzsczb8m 73 4 the the DT cord-270326-fzsczb8m 73 5 species specie NNS cord-270326-fzsczb8m 73 6 specificity specificity NN cord-270326-fzsczb8m 73 7 of of IN cord-270326-fzsczb8m 73 8 the the DT cord-270326-fzsczb8m 73 9 receptor receptor NN cord-270326-fzsczb8m 73 10 molecule molecule NN cord-270326-fzsczb8m 73 11 , , , cord-270326-fzsczb8m 73 12 present present JJ cord-270326-fzsczb8m 73 13 also also RB cord-270326-fzsczb8m 73 14 in in IN cord-270326-fzsczb8m 73 15 astrocytes astrocyte NNS cord-270326-fzsczb8m 73 16 . . . cord-270326-fzsczb8m 74 1 The the DT cord-270326-fzsczb8m 74 2 above above JJ cord-270326-fzsczb8m 74 3 results result NNS cord-270326-fzsczb8m 74 4 provide provide VBP cord-270326-fzsczb8m 74 5 direct direct JJ cord-270326-fzsczb8m 74 6 evidence evidence NN cord-270326-fzsczb8m 74 7 for for IN cord-270326-fzsczb8m 74 8 high high JJ cord-270326-fzsczb8m 74 9 affinity affinity NN cord-270326-fzsczb8m 74 10 binding binding NN cord-270326-fzsczb8m 74 11 of of IN cord-270326-fzsczb8m 74 12 ' ' `` cord-270326-fzsczb8m 74 13 immune immune JJ cord-270326-fzsczb8m 74 14 ' ' '' cord-270326-fzsczb8m 74 15 interferon interferon NN cord-270326-fzsczb8m 74 16 to to IN cord-270326-fzsczb8m 74 17 a a DT cord-270326-fzsczb8m 74 18 specific specific JJ cord-270326-fzsczb8m 74 19 cell cell NN cord-270326-fzsczb8m 74 20 surface surface NN cord-270326-fzsczb8m 74 21 receptor receptor NN cord-270326-fzsczb8m 74 22 on on IN cord-270326-fzsczb8m 74 23 murine murine JJ cord-270326-fzsczb8m 74 24 astro- astro- NNP cord-270326-fzsczb8m 74 25 _SP cord-270326-fzsczb8m 75 1 The the DT cord-270326-fzsczb8m 75 2 use use NN cord-270326-fzsczb8m 75 3 of of IN cord-270326-fzsczb8m 75 4 a a DT cord-270326-fzsczb8m 75 5 rabbit rabbit NN cord-270326-fzsczb8m 75 6 antibody antibody NN cord-270326-fzsczb8m 75 7 against against IN cord-270326-fzsczb8m 75 8 murine murine JJ cord-270326-fzsczb8m 75 9 IFN IFN NNP cord-270326-fzsczb8m 75 10 - - HYPH cord-270326-fzsczb8m 75 11 T T NNP cord-270326-fzsczb8m 75 12 and and CC cord-270326-fzsczb8m 75 13 125I 125I NNP cord-270326-fzsczb8m 75 14 - - HYPH cord-270326-fzsczb8m 75 15 labeled label VBN cord-270326-fzsczb8m 75 16 protein protein NN cord-270326-fzsczb8m 75 17 A A NNP cord-270326-fzsczb8m 75 18 allows allow VBZ cord-270326-fzsczb8m 75 19 us -PRON- PRP cord-270326-fzsczb8m 75 20 to to TO cord-270326-fzsczb8m 75 21 study study VB cord-270326-fzsczb8m 75 22 the the DT cord-270326-fzsczb8m 75 23 availability availability NN cord-270326-fzsczb8m 75 24 of of IN cord-270326-fzsczb8m 75 25 the the DT cord-270326-fzsczb8m 75 26 leukin leukin NNP cord-270326-fzsczb8m 75 27 on on IN cord-270326-fzsczb8m 75 28 the the DT cord-270326-fzsczb8m 75 29 astrocyte astrocyte NNP cord-270326-fzsczb8m 75 30 surface surface NN cord-270326-fzsczb8m 75 31 . . . cord-270326-fzsczb8m 76 1 When when WRB cord-270326-fzsczb8m 76 2 cell cell NN cord-270326-fzsczb8m 76 3 receptor receptor NN cord-270326-fzsczb8m 76 4 - - HYPH cord-270326-fzsczb8m 76 5 bound bind VBN cord-270326-fzsczb8m 76 6 unlabeled unlabeled JJ cord-270326-fzsczb8m 76 7 IFN IFN NNP cord-270326-fzsczb8m 76 8 - - HYPH cord-270326-fzsczb8m 76 9 T T NNP cord-270326-fzsczb8m 76 10 remains remain VBZ cord-270326-fzsczb8m 76 11 at at IN cord-270326-fzsczb8m 76 12 37 37 CD cord-270326-fzsczb8m 76 13 ° ° NN cord-270326-fzsczb8m 76 14 C c NN cord-270326-fzsczb8m 76 15 in in IN cord-270326-fzsczb8m 76 16 complete complete JJ cord-270326-fzsczb8m 76 17 culture culture NN cord-270326-fzsczb8m 76 18 medium medium NN cord-270326-fzsczb8m 76 19 ( ( -LRB- cord-270326-fzsczb8m 76 20 DMEM DMEM NNP cord-270326-fzsczb8m 76 21 + + CC cord-270326-fzsczb8m 77 1 10 10 CD cord-270326-fzsczb8m 77 2 % % NN cord-270326-fzsczb8m 77 3 FCS FCS NNP cord-270326-fzsczb8m 77 4 ) ) -RRB- cord-270326-fzsczb8m 78 1 it -PRON- PRP cord-270326-fzsczb8m 78 2 begins begin VBZ cord-270326-fzsczb8m 78 3 to to TO cord-270326-fzsczb8m 78 4 be be VB cord-270326-fzsczb8m 78 5 internalized internalize VBN cord-270326-fzsczb8m 78 6 after after IN cord-270326-fzsczb8m 78 7 30 30 CD cord-270326-fzsczb8m 78 8 min min NN cord-270326-fzsczb8m 78 9 . . . cord-270326-fzsczb8m 79 1 After after IN cord-270326-fzsczb8m 79 2 4 4 CD cord-270326-fzsczb8m 79 3 h h NN cord-270326-fzsczb8m 79 4 only only RB cord-270326-fzsczb8m 79 5 50 50 CD cord-270326-fzsczb8m 79 6 % % NN cord-270326-fzsczb8m 79 7 of of IN cord-270326-fzsczb8m 79 8 the the DT cord-270326-fzsczb8m 79 9 maximal maximal JJ cord-270326-fzsczb8m 79 10 amount amount NN cord-270326-fzsczb8m 79 11 of of IN cord-270326-fzsczb8m 79 12 bound bind VBN cord-270326-fzsczb8m 79 13 IFN IFN NNP cord-270326-fzsczb8m 79 14 - - HYPH cord-270326-fzsczb8m 79 15 T T NNP cord-270326-fzsczb8m 79 16 remains remain VBZ cord-270326-fzsczb8m 79 17 available available JJ cord-270326-fzsczb8m 79 18 to to IN cord-270326-fzsczb8m 79 19 the the DT cord-270326-fzsczb8m 79 20 specific specific JJ cord-270326-fzsczb8m 79 21 antibody antibody NN cord-270326-fzsczb8m 79 22 plus plus CC cord-270326-fzsczb8m 79 23 protein protein NN cord-270326-fzsczb8m 79 24 A a DT cord-270326-fzsczb8m 79 25 complex complex NN cord-270326-fzsczb8m 79 26 . . . cord-270326-fzsczb8m 80 1 If if IN cord-270326-fzsczb8m 80 2 the the DT cord-270326-fzsczb8m 80 3 binding binding NN cord-270326-fzsczb8m 80 4 and and CC cord-270326-fzsczb8m 80 5 further further JJ cord-270326-fzsczb8m 80 6 incubation incubation NN cord-270326-fzsczb8m 80 7 are be VBP cord-270326-fzsczb8m 80 8 performed perform VBN cord-270326-fzsczb8m 80 9 at at IN cord-270326-fzsczb8m 80 10 4 4 CD cord-270326-fzsczb8m 80 11 ° ° NN cord-270326-fzsczb8m 80 12 C c NN cord-270326-fzsczb8m 80 13 , , , cord-270326-fzsczb8m 80 14 internalization internalization NN cord-270326-fzsczb8m 80 15 does do VBZ cord-270326-fzsczb8m 80 16 not not RB cord-270326-fzsczb8m 80 17 occur occur VB cord-270326-fzsczb8m 80 18 ( ( -LRB- cord-270326-fzsczb8m 80 19 Fig fig NN cord-270326-fzsczb8m 80 20 . . . cord-270326-fzsczb8m 80 21 4 4 CD cord-270326-fzsczb8m 80 22 ) ) -RRB- cord-270326-fzsczb8m 80 23 . . . cord-270326-fzsczb8m 81 1 No no DT cord-270326-fzsczb8m 81 2 endocytosis endocytosis NN cord-270326-fzsczb8m 81 3 takes take VBZ cord-270326-fzsczb8m 81 4 place place NN cord-270326-fzsczb8m 81 5 when when WRB cord-270326-fzsczb8m 81 6 the the DT cord-270326-fzsczb8m 81 7 complete complete JJ cord-270326-fzsczb8m 81 8 culture culture NN cord-270326-fzsczb8m 81 9 medium medium NN cord-270326-fzsczb8m 81 10 is be VBZ cord-270326-fzsczb8m 81 11 substituted substitute VBN cord-270326-fzsczb8m 81 12 by by IN cord-270326-fzsczb8m 81 13 PBS PBS NNP cord-270326-fzsczb8m 81 14 . . . cord-270326-fzsczb8m 82 1 We -PRON- PRP cord-270326-fzsczb8m 82 2 assume assume VBP cord-270326-fzsczb8m 82 3 that that IN cord-270326-fzsczb8m 82 4 the the DT cord-270326-fzsczb8m 82 5 detection detection NN cord-270326-fzsczb8m 82 6 of of IN cord-270326-fzsczb8m 82 7 lower low JJR cord-270326-fzsczb8m 82 8 antibody antibody NN cord-270326-fzsczb8m 82 9 binding binding NN cord-270326-fzsczb8m 82 10 is be VBZ cord-270326-fzsczb8m 82 11 due due JJ cord-270326-fzsczb8m 82 12 to to IN cord-270326-fzsczb8m 82 13 an an DT cord-270326-fzsczb8m 82 14 energy energy NN cord-270326-fzsczb8m 82 15 - - HYPH cord-270326-fzsczb8m 82 16 dependent dependent JJ cord-270326-fzsczb8m 82 17 in- in- XX cord-270326-fzsczb8m 82 18 ternalization ternalization NN cord-270326-fzsczb8m 82 19 process process NN cord-270326-fzsczb8m 82 20 rather rather RB cord-270326-fzsczb8m 82 21 than than IN cord-270326-fzsczb8m 82 22 to to IN cord-270326-fzsczb8m 82 23 a a DT cord-270326-fzsczb8m 82 24 release release NN cord-270326-fzsczb8m 82 25 from from IN cord-270326-fzsczb8m 82 26 the the DT cord-270326-fzsczb8m 82 27 cell cell NN cord-270326-fzsczb8m 82 28 receptor receptor NN cord-270326-fzsczb8m 82 29 , , , cord-270326-fzsczb8m 82 30 because because IN cord-270326-fzsczb8m 82 31 of of IN cord-270326-fzsczb8m 82 32 the the DT cord-270326-fzsczb8m 82 33 high high JJ cord-270326-fzsczb8m 82 34 affinity affinity NN cord-270326-fzsczb8m 82 35 of of IN cord-270326-fzsczb8m 82 36 the the DT cord-270326-fzsczb8m 82 37 binding binding NN cord-270326-fzsczb8m 82 38 ( ( -LRB- cord-270326-fzsczb8m 82 39 K K NNP cord-270326-fzsczb8m 82 40 a a NN cord-270326-fzsczb8m 82 41 = = SYM cord-270326-fzsczb8m 82 42 1.64 1.64 CD cord-270326-fzsczb8m 82 43 × × NN cord-270326-fzsczb8m 82 44 10 10 CD cord-270326-fzsczb8m 82 45 -9 -9 NNP cord-270326-fzsczb8m 82 46 M M NNP cord-270326-fzsczb8m 82 47 ) ) -RRB- cord-270326-fzsczb8m 82 48 and and CC cord-270326-fzsczb8m 82 49 be- be- NNP cord-270326-fzsczb8m 82 50 causc causc NNP cord-270326-fzsczb8m 83 1 the the DT cord-270326-fzsczb8m 83 2 hypothetic hypothetic JJ cord-270326-fzsczb8m 83 3 release release NN cord-270326-fzsczb8m 83 4 does do VBZ cord-270326-fzsczb8m 83 5 not not RB cord-270326-fzsczb8m 83 6 take take VB cord-270326-fzsczb8m 83 7 place place NN cord-270326-fzsczb8m 83 8 either either CC cord-270326-fzsczb8m 83 9 at at IN cord-270326-fzsczb8m 83 10 4 4 CD cord-270326-fzsczb8m 83 11 ° ° NN cord-270326-fzsczb8m 83 12 C c NN cord-270326-fzsczb8m 83 13 or or CC cord-270326-fzsczb8m 83 14 in in IN cord-270326-fzsczb8m 83 15 PBS PBS NNP cord-270326-fzsczb8m 83 16 at at IN cord-270326-fzsczb8m 83 17 37 37 CD cord-270326-fzsczb8m 83 18 ° ° NN cord-270326-fzsczb8m 83 19 C c NN cord-270326-fzsczb8m 83 20 . . . cord-270326-fzsczb8m 84 1 These these DT cord-270326-fzsczb8m 84 2 data datum NNS cord-270326-fzsczb8m 84 3 demonstrated demonstrate VBD cord-270326-fzsczb8m 84 4 an an DT cord-270326-fzsczb8m 84 5 energy energy NN cord-270326-fzsczb8m 84 6 - - HYPH cord-270326-fzsczb8m 84 7 dependent dependent JJ cord-270326-fzsczb8m 84 8 internalization internalization NN cord-270326-fzsczb8m 84 9 of of IN cord-270326-fzsczb8m 84 10 IFN-,/ ifn-,/ NN cord-270326-fzsczb8m 84 11 by by IN cord-270326-fzsczb8m 84 12 astrocytes astrocyte NNS cord-270326-fzsczb8m 84 13 , , , cord-270326-fzsczb8m 84 14 as as IN cord-270326-fzsczb8m 84 15 described describe VBN cord-270326-fzsczb8m 84 16 by by IN cord-270326-fzsczb8m 84 17 others other NNS cord-270326-fzsczb8m 84 18 for for IN cord-270326-fzsczb8m 84 19 macrophages macrophage NNS cord-270326-fzsczb8m 84 20 ( ( -LRB- cord-270326-fzsczb8m 84 21 Anderson Anderson NNP cord-270326-fzsczb8m 84 22 eta eta NNP cord-270326-fzsczb8m 84 23 [ [ -LRB- cord-270326-fzsczb8m 84 24 . . NNP cord-270326-fzsczb8m 84 25 , , , cord-270326-fzsczb8m 84 26 1983 1983 CD cord-270326-fzsczb8m 84 27 ) ) -RRB- cord-270326-fzsczb8m 84 28 . . . cord-270326-fzsczb8m 85 1 To to TO cord-270326-fzsczb8m 85 2 further further RB cord-270326-fzsczb8m 85 3 demonstrate demonstrate VB cord-270326-fzsczb8m 85 4 internalization internalization NN cord-270326-fzsczb8m 85 5 of of IN cord-270326-fzsczb8m 85 6 the the DT cord-270326-fzsczb8m 85 7 ligand ligand NN cord-270326-fzsczb8m 85 8 , , , cord-270326-fzsczb8m 85 9 we -PRON- PRP cord-270326-fzsczb8m 85 10 assessed assess VBD cord-270326-fzsczb8m 85 11 the the DT cord-270326-fzsczb8m 85 12 amount amount NN cord-270326-fzsczb8m 85 13 of of IN cord-270326-fzsczb8m 85 14 intracellular intracellular JJ cord-270326-fzsczb8m 85 15 radioactivity radioactivity NN cord-270326-fzsczb8m 85 16 after after IN cord-270326-fzsczb8m 85 17 removing remove VBG cord-270326-fzsczb8m 85 18 surface surface NN cord-270326-fzsczb8m 85 19 - - HYPH cord-270326-fzsczb8m 85 20 bound bind VBN cord-270326-fzsczb8m 85 21 INF INF NNP cord-270326-fzsczb8m 85 22 - - HYPH cord-270326-fzsczb8m 85 23 T T NNP cord-270326-fzsczb8m 85 24 at at IN cord-270326-fzsczb8m 85 25 low low JJ cord-270326-fzsczb8m 85 26 pH ph NN cord-270326-fzsczb8m 85 27 ( ( -LRB- cord-270326-fzsczb8m 85 28 glycine glycine NN cord-270326-fzsczb8m 85 29 buffer buffer NN cord-270326-fzsczb8m 85 30 , , , cord-270326-fzsczb8m 85 31 pH pH NNP cord-270326-fzsczb8m 85 32 3.0 3.0 CD cord-270326-fzsczb8m 85 33 ) ) -RRB- cord-270326-fzsczb8m 85 34 . . . cord-270326-fzsczb8m 86 1 Table table NN cord-270326-fzsczb8m 86 2 1 1 CD cord-270326-fzsczb8m 86 3 shows show VBZ cord-270326-fzsczb8m 86 4 that that IN cord-270326-fzsczb8m 86 5 at at IN cord-270326-fzsczb8m 86 6 4 4 CD cord-270326-fzsczb8m 86 7 ° ° NN cord-270326-fzsczb8m 86 8 C c NN cord-270326-fzsczb8m 86 9 no no DT cord-270326-fzsczb8m 86 10 internalization internalization NN cord-270326-fzsczb8m 86 11 takes take VBZ cord-270326-fzsczb8m 86 12 place place NN cord-270326-fzsczb8m 86 13 in in IN cord-270326-fzsczb8m 86 14 a a DT cord-270326-fzsczb8m 86 15 significant significant JJ cord-270326-fzsczb8m 86 16 manner manner NN cord-270326-fzsczb8m 86 17 when when WRB cord-270326-fzsczb8m 86 18 the the DT cord-270326-fzsczb8m 86 19 time time NN cord-270326-fzsczb8m 86 20 of of IN cord-270326-fzsczb8m 86 21 incubation incubation NN cord-270326-fzsczb8m 86 22 is be VBZ cord-270326-fzsczb8m 86 23 increased increase VBN cord-270326-fzsczb8m 86 24 . . . cord-270326-fzsczb8m 87 1 Conversely conversely RB cord-270326-fzsczb8m 87 2 , , , cord-270326-fzsczb8m 87 3 at at IN cord-270326-fzsczb8m 87 4 37 37 CD cord-270326-fzsczb8m 87 5 ° ° NN cord-270326-fzsczb8m 87 6 C C NNP cord-270326-fzsczb8m 87 7 the the DT cord-270326-fzsczb8m 87 8 pH pH NNP cord-270326-fzsczb8m 87 9 3.0-resistant 3.0-resistant CD cord-270326-fzsczb8m 87 10 label label NN cord-270326-fzsczb8m 87 11 ( ( -LRB- cord-270326-fzsczb8m 87 12 internalized internalize VBN cord-270326-fzsczb8m 87 13 ) ) -RRB- cord-270326-fzsczb8m 87 14 increases increase NNS cord-270326-fzsczb8m 87 15 , , , cord-270326-fzsczb8m 87 16 reaching reach VBG cord-270326-fzsczb8m 87 17 56 56 CD cord-270326-fzsczb8m 87 18 _ _ NN cord-270326-fzsczb8m 87 19 + + SYM cord-270326-fzsczb8m 87 20 5.2 5.2 CD cord-270326-fzsczb8m 87 21 % % NN cord-270326-fzsczb8m 87 22 of of IN cord-270326-fzsczb8m 87 23 maximal maximal JJ cord-270326-fzsczb8m 87 24 binding binding NN cord-270326-fzsczb8m 87 25 after after IN cord-270326-fzsczb8m 87 26 240 240 CD cord-270326-fzsczb8m 87 27 min min NN cord-270326-fzsczb8m 87 28 of of IN cord-270326-fzsczb8m 87 29 incubation incubation NN cord-270326-fzsczb8m 87 30 . . . cord-270326-fzsczb8m 88 1 The the DT cord-270326-fzsczb8m 88 2 amount amount NN cord-270326-fzsczb8m 88 3 of of IN cord-270326-fzsczb8m 88 4 cpm cpm NN cord-270326-fzsczb8m 88 5 bound bind VBN cord-270326-fzsczb8m 88 6 after after IN cord-270326-fzsczb8m 88 7 30 30 CD cord-270326-fzsczb8m 88 8 min min NN cord-270326-fzsczb8m 88 9 at at IN cord-270326-fzsczb8m 88 10 37 37 CD cord-270326-fzsczb8m 88 11 ° ° NN cord-270326-fzsczb8m 88 12 C c NN cord-270326-fzsczb8m 88 13 is be VBZ cord-270326-fzsczb8m 88 14 defined define VBN cord-270326-fzsczb8m 88 15 as as IN cord-270326-fzsczb8m 88 16 100 100 CD cord-270326-fzsczb8m 88 17 % % NN cord-270326-fzsczb8m 88 18 of of IN cord-270326-fzsczb8m 88 19 binding bind VBG cord-270326-fzsczb8m 88 20 . . . cord-270326-fzsczb8m 89 1 Interferon interferon NN cord-270326-fzsczb8m 89 2 gamma gamma NN cord-270326-fzsczb8m 89 3 is be VBZ cord-270326-fzsczb8m 89 4 a a DT cord-270326-fzsczb8m 89 5 lymphokine lymphokine NN cord-270326-fzsczb8m 89 6 with with IN cord-270326-fzsczb8m 89 7 a a DT cord-270326-fzsczb8m 89 8 wide wide JJ cord-270326-fzsczb8m 89 9 array array NN cord-270326-fzsczb8m 89 10 of of IN cord-270326-fzsczb8m 89 11 biological biological JJ cord-270326-fzsczb8m 89 12 effects effect NNS cord-270326-fzsczb8m 89 13 on on IN cord-270326-fzsczb8m 89 14 immune immune JJ cord-270326-fzsczb8m 89 15 and and CC cord-270326-fzsczb8m 89 16 nonimmune nonimmune JJ cord-270326-fzsczb8m 89 17 cells cell NNS cord-270326-fzsczb8m 89 18 ( ( -LRB- cord-270326-fzsczb8m 89 19 Trinchieri Trinchieri NNP cord-270326-fzsczb8m 89 20 and and CC cord-270326-fzsczb8m 89 21 Perussia Perussia NNP cord-270326-fzsczb8m 89 22 , , , cord-270326-fzsczb8m 89 23 1985 1985 CD cord-270326-fzsczb8m 89 24 ) ) -RRB- cord-270326-fzsczb8m 89 25 . . . cord-270326-fzsczb8m 90 1 These these DT cord-270326-fzsczb8m 90 2 effects effect NNS cord-270326-fzsczb8m 90 3 are be VBP cord-270326-fzsczb8m 90 4 thought think VBN cord-270326-fzsczb8m 90 5 to to TO cord-270326-fzsczb8m 90 6 be be VB cord-270326-fzsczb8m 90 7 mediated mediate VBN cord-270326-fzsczb8m 90 8 by by IN cord-270326-fzsczb8m 90 9 a a DT cord-270326-fzsczb8m 90 10 cell cell NN cord-270326-fzsczb8m 90 11 surface surface NN cord-270326-fzsczb8m 90 12 receptor receptor NN cord-270326-fzsczb8m 90 13 ( ( -LRB- cord-270326-fzsczb8m 90 14 Aguet Aguet NNP cord-270326-fzsczb8m 90 15 , , , cord-270326-fzsczb8m 90 16 1980 1980 CD cord-270326-fzsczb8m 90 17 ) ) -RRB- cord-270326-fzsczb8m 90 18 which which WDT cord-270326-fzsczb8m 90 19 does do VBZ cord-270326-fzsczb8m 90 20 not not RB cord-270326-fzsczb8m 90 21 bind bind VB cord-270326-fzsczb8m 90 22 IFN IFN NNP cord-270326-fzsczb8m 90 23 - - HYPH cord-270326-fzsczb8m 90 24 a a NN cord-270326-fzsczb8m 90 25 or or CC cord-270326-fzsczb8m 90 26 IFN-/3 ifn-/3 NN cord-270326-fzsczb8m 90 27 ( ( -LRB- cord-270326-fzsczb8m 90 28 Branca Branca NNP cord-270326-fzsczb8m 90 29 and and CC cord-270326-fzsczb8m 90 30 Baglioni Baglioni NNP cord-270326-fzsczb8m 90 31 , , , cord-270326-fzsczb8m 90 32 1981 1981 CD cord-270326-fzsczb8m 90 33 ) ) -RRB- cord-270326-fzsczb8m 90 34 . . . cord-270326-fzsczb8m 91 1 The the DT cord-270326-fzsczb8m 91 2 human human JJ cord-270326-fzsczb8m 91 3 IFN IFN NNP cord-270326-fzsczb8m 91 4 - - HYPH cord-270326-fzsczb8m 91 5 y y NNP cord-270326-fzsczb8m 91 6 receptor receptor NN cord-270326-fzsczb8m 91 7 has have VBZ cord-270326-fzsczb8m 91 8 been be VBN cord-270326-fzsczb8m 91 9 purified purify VBN cord-270326-fzsczb8m 91 10 and and CC cord-270326-fzsczb8m 91 11 characterized characterize VBN cord-270326-fzsczb8m 91 12 from from IN cord-270326-fzsczb8m 91 13 a a DT cord-270326-fzsczb8m 91 14 biochemical biochemical JJ cord-270326-fzsczb8m 91 15 point point NN cord-270326-fzsczb8m 91 16 of of IN cord-270326-fzsczb8m 91 17 view view NN cord-270326-fzsczb8m 91 18 showing show VBG cord-270326-fzsczb8m 91 19 a a DT cord-270326-fzsczb8m 91 20 molecular molecular JJ cord-270326-fzsczb8m 91 21 weight weight NN cord-270326-fzsczb8m 91 22 of of IN cord-270326-fzsczb8m 91 23 around around RB cord-270326-fzsczb8m 91 24 9(I,000 9(i,000 CD cord-270326-fzsczb8m 92 1 ( ( -LRB- cord-270326-fzsczb8m 92 2 Novick Novick NNP cord-270326-fzsczb8m 92 3 et et NNP cord-270326-fzsczb8m 92 4 al al NNP cord-270326-fzsczb8m 92 5 . . NNP cord-270326-fzsczb8m 92 6 , , , cord-270326-fzsczb8m 92 7 1987 1987 CD cord-270326-fzsczb8m 93 1 ( ( -LRB- cord-270326-fzsczb8m 93 2 Novick Novick NNP cord-270326-fzsczb8m 93 3 et et NNP cord-270326-fzsczb8m 93 4 al al NNP cord-270326-fzsczb8m 93 5 . . NNP cord-270326-fzsczb8m 93 6 , , , cord-270326-fzsczb8m 93 7 , , , cord-270326-fzsczb8m 93 8 1989 1989 CD cord-270326-fzsczb8m 93 9 . . . cord-270326-fzsczb8m 94 1 Monoclonal monoclonal JJ cord-270326-fzsczb8m 94 2 antibodies antibody NNS cord-270326-fzsczb8m 94 3 with with IN cord-270326-fzsczb8m 94 4 the the DT cord-270326-fzsczb8m 94 5 ability ability NN cord-270326-fzsczb8m 94 6 to to TO cord-270326-fzsczb8m 94 7 block block VB cord-270326-fzsczb8m 94 8 the the DT cord-270326-fzsczb8m 94 9 binding binding NN cord-270326-fzsczb8m 94 10 of of IN cord-270326-fzsczb8m 94 11 I25I I25I NNP cord-270326-fzsczb8m 94 12 - - HYPH cord-270326-fzsczb8m 94 13 labeled label VBN cord-270326-fzsczb8m 94 14 IFN-,/to ifn-,/to IN cord-270326-fzsczb8m 94 15 its -PRON- PRP$ cord-270326-fzsczb8m 94 16 receptor receptor NN cord-270326-fzsczb8m 94 17 have have VBP cord-270326-fzsczb8m 94 18 been be VBN cord-270326-fzsczb8m 94 19 raised raise VBN cord-270326-fzsczb8m 94 20 against against IN cord-270326-fzsczb8m 94 21 it -PRON- PRP cord-270326-fzsczb8m 95 1 ( ( -LRB- cord-270326-fzsczb8m 95 2 Novick Novick NNP cord-270326-fzsczb8m 95 3 et et NNP cord-270326-fzsczb8m 95 4 al al NNP cord-270326-fzsczb8m 95 5 . . . cord-270326-fzsczb8m 96 1 , , , cord-270326-fzsczb8m 96 2 1989 1989 CD cord-270326-fzsczb8m 96 3 ; ; : cord-270326-fzsczb8m 96 4 Depla Depla NNP cord-270326-fzsczb8m 96 5 and and CC cord-270326-fzsczb8m 96 6 De De NNP cord-270326-fzsczb8m 96 7 Ley Ley NNP cord-270326-fzsczb8m 96 8 , , , cord-270326-fzsczb8m 96 9 1990 1990 CD cord-270326-fzsczb8m 96 10 ) ) -RRB- cord-270326-fzsczb8m 96 11 . . . cord-270326-fzsczb8m 97 1 Finally finally RB cord-270326-fzsczb8m 97 2 , , , cord-270326-fzsczb8m 97 3 the the DT cord-270326-fzsczb8m 97 4 gene gene NN cord-270326-fzsczb8m 97 5 encoding encode VBG cord-270326-fzsczb8m 97 6 the the DT cord-270326-fzsczb8m 97 7 human human JJ cord-270326-fzsczb8m 97 8 IFNreceptor IFNreceptor NNP cord-270326-fzsczb8m 97 9 has have VBZ cord-270326-fzsczb8m 97 10 recently recently RB cord-270326-fzsczb8m 97 11 been be VBN cord-270326-fzsczb8m 97 12 cloned clone VBN cord-270326-fzsczb8m 97 13 ( ( -LRB- cord-270326-fzsczb8m 97 14 Aguet Aguet NNP cord-270326-fzsczb8m 97 15 et et NNP cord-270326-fzsczb8m 97 16 al al NNP cord-270326-fzsczb8m 97 17 . . NNP cord-270326-fzsczb8m 97 18 , , , cord-270326-fzsczb8m 97 19 1988 1988 CD cord-270326-fzsczb8m 97 20 ) ) -RRB- cord-270326-fzsczb8m 97 21 . . . cord-270326-fzsczb8m 98 1 In in IN cord-270326-fzsczb8m 98 2 addition addition NN cord-270326-fzsczb8m 98 3 to to IN cord-270326-fzsczb8m 98 4 the the DT cord-270326-fzsczb8m 98 5 macrophages macrophage NNS cord-270326-fzsczb8m 98 6 , , , cord-270326-fzsczb8m 98 7 its -PRON- PRP$ cord-270326-fzsczb8m 98 8 main main JJ cord-270326-fzsczb8m 98 9 target target NN cord-270326-fzsczb8m 98 10 cell cell NN cord-270326-fzsczb8m 98 11 ( ( -LRB- cord-270326-fzsczb8m 98 12 Schreiber Schreiber NNP cord-270326-fzsczb8m 98 13 et et FW cord-270326-fzsczb8m 98 14 al al NNP cord-270326-fzsczb8m 98 15 . . NNP cord-270326-fzsczb8m 98 16 , , , cord-270326-fzsczb8m 98 17 1985 1985 CD cord-270326-fzsczb8m 98 18 ) ) -RRB- cord-270326-fzsczb8m 98 19 , , , cord-270326-fzsczb8m 98 20 glial glial JJ cord-270326-fzsczb8m 98 21 astrocytes astrocyte NNS cord-270326-fzsczb8m 98 22 have have VBP cord-270326-fzsczb8m 98 23 been be VBN cord-270326-fzsczb8m 98 24 reported report VBN cord-270326-fzsczb8m 98 25 to to TO cord-270326-fzsczb8m 98 26 be be VB cord-270326-fzsczb8m 98 27 committed commit VBN cord-270326-fzsczb8m 98 28 to to IN cord-270326-fzsczb8m 98 29 an an DT cord-270326-fzsczb8m 98 30 important important JJ cord-270326-fzsczb8m 98 31 immune immune JJ cord-270326-fzsczb8m 98 32 role role NN cord-270326-fzsczb8m 98 33 when when WRB cord-270326-fzsczb8m 98 34 in in IN cord-270326-fzsczb8m 98 35 contact contact NN cord-270326-fzsczb8m 98 36 with with IN cord-270326-fzsczb8m 98 37 IFN IFN NNP cord-270326-fzsczb8m 98 38 - - HYPH cord-270326-fzsczb8m 98 39 y y NNP cord-270326-fzsczb8m 98 40 ( ( -LRB- cord-270326-fzsczb8m 98 41 Hirsch Hirsch NNP cord-270326-fzsczb8m 98 42 et et NNP cord-270326-fzsczb8m 98 43 al al NNP cord-270326-fzsczb8m 98 44 . . . cord-270326-fzsczb8m 99 1 , , , cord-270326-fzsczb8m 99 2 1983 1983 CD cord-270326-fzsczb8m 99 3 ; ; : cord-270326-fzsczb8m 99 4 Wong Wong NNP cord-270326-fzsczb8m 99 5 et et NNP cord-270326-fzsczb8m 99 6 al al NNP cord-270326-fzsczb8m 99 7 . . NNP cord-270326-fzsczb8m 99 8 , , , cord-270326-fzsczb8m 99 9 1985 1985 CD cord-270326-fzsczb8m 99 10 ) ) -RRB- cord-270326-fzsczb8m 99 11 . . . cord-270326-fzsczb8m 100 1 Our -PRON- PRP$ cord-270326-fzsczb8m 100 2 results result NNS cord-270326-fzsczb8m 100 3 clearly clearly RB cord-270326-fzsczb8m 100 4 demonstrate demonstrate VBP cord-270326-fzsczb8m 100 5 the the DT cord-270326-fzsczb8m 100 6 presence presence NN cord-270326-fzsczb8m 100 7 of of IN cord-270326-fzsczb8m 100 8 a a DT cord-270326-fzsczb8m 100 9 specific specific JJ cord-270326-fzsczb8m 100 10 receptor receptor NN cord-270326-fzsczb8m 100 11 for for IN cord-270326-fzsczb8m 100 12 IFN--/ IFN--/ NNP cord-270326-fzsczb8m 100 13 on on IN cord-270326-fzsczb8m 100 14 murine murine JJ cord-270326-fzsczb8m 100 15 astrocytes astrocyte NNS cord-270326-fzsczb8m 100 16 . . . cord-270326-fzsczb8m 101 1 The the DT cord-270326-fzsczb8m 101 2 binding binding NN cord-270326-fzsczb8m 101 3 takes take VBZ cord-270326-fzsczb8m 101 4 place place NN cord-270326-fzsczb8m 101 5 in in IN cord-270326-fzsczb8m 101 6 a a DT cord-270326-fzsczb8m 101 7 bimolecular bimolecular JJ cord-270326-fzsczb8m 101 8 reversible reversible JJ cord-270326-fzsczb8m 101 9 manner manner NN cord-270326-fzsczb8m 101 10 with with IN cord-270326-fzsczb8m 101 11 high high JJ cord-270326-fzsczb8m 101 12 affinity affinity NN cord-270326-fzsczb8m 101 13 , , , cord-270326-fzsczb8m 101 14 K~ K~ NNP cord-270326-fzsczb8m 101 15 = = SYM cord-270326-fzsczb8m 101 16 1.64 1.64 CD cord-270326-fzsczb8m 101 17 × × NN cord-270326-fzsczb8m 102 1 10 10 CD cord-270326-fzsczb8m 102 2 .9 .9 CD cord-270326-fzsczb8m 102 3 M. m. NN cord-270326-fzsczb8m 103 1 This this DT cord-270326-fzsczb8m 103 2 affinity affinity NN cord-270326-fzsczb8m 103 3 constant constant NN cord-270326-fzsczb8m 103 4 is be VBZ cord-270326-fzsczb8m 103 5 higher high JJR cord-270326-fzsczb8m 103 6 than than IN cord-270326-fzsczb8m 103 7 that that DT cord-270326-fzsczb8m 103 8 found find VBN cord-270326-fzsczb8m 103 9 for for IN cord-270326-fzsczb8m 103 10 rlFN rlFN NNP cord-270326-fzsczb8m 103 11 - - HYPH cord-270326-fzsczb8m 103 12 y y NNP cord-270326-fzsczb8m 103 13 on on IN cord-270326-fzsczb8m 103 14 human human JJ cord-270326-fzsczb8m 103 15 fibroblasts fibroblast NNS cord-270326-fzsczb8m 103 16 ( ( -LRB- cord-270326-fzsczb8m 103 17 1 1 CD cord-270326-fzsczb8m 103 18 - - SYM cord-270326-fzsczb8m 103 19 5 5 CD cord-270326-fzsczb8m 103 20 × × NNP cord-270326-fzsczb8m 103 21 10 10 CD cord-270326-fzsczb8m 103 22 -s -s NNP cord-270326-fzsczb8m 103 23 M M NNP cord-270326-fzsczb8m 103 24 ) ) -RRB- cord-270326-fzsczb8m 104 1 ( ( -LRB- cord-270326-fzsczb8m 104 2 Anderson Anderson NNP cord-270326-fzsczb8m 104 3 et et NNP cord-270326-fzsczb8m 104 4 al al NNP cord-270326-fzsczb8m 104 5 . . NNP cord-270326-fzsczb8m 104 6 , , , cord-270326-fzsczb8m 104 7 1982 1982 CD cord-270326-fzsczb8m 104 8 ) ) -RRB- cord-270326-fzsczb8m 104 9 and and CC cord-270326-fzsczb8m 104 10 mouse mouse NN cord-270326-fzsczb8m 104 11 macrophages macrophage NNS cord-270326-fzsczb8m 104 12 ( ( -LRB- cord-270326-fzsczb8m 104 13 1.18 1.18 CD cord-270326-fzsczb8m 104 14 × × NN cord-270326-fzsczb8m 104 15 10 10 CD cord-270326-fzsczb8m 104 16 -s -s NNP cord-270326-fzsczb8m 104 17 M M NNP cord-270326-fzsczb8m 104 18 ) ) -RRB- cord-270326-fzsczb8m 105 1 ( ( -LRB- cord-270326-fzsczb8m 105 2 Celada Celada NNP cord-270326-fzsczb8m 105 3 et et NNP cord-270326-fzsczb8m 105 4 al al NNP cord-270326-fzsczb8m 105 5 . . NNP cord-270326-fzsczb8m 105 6 , , , cord-270326-fzsczb8m 105 7 1984 1984 CD cord-270326-fzsczb8m 105 8 ) ) -RRB- cord-270326-fzsczb8m 105 9 and and CC cord-270326-fzsczb8m 105 10 lower low JJR cord-270326-fzsczb8m 105 11 than than IN cord-270326-fzsczb8m 105 12 that that DT cord-270326-fzsczb8m 105 13 rcportcd rcportcd NN cord-270326-fzsczb8m 105 14 for for IN cord-270326-fzsczb8m 105 15 human human JJ cord-270326-fzsczb8m 105 16 monocytes monocyte NNS cord-270326-fzsczb8m 105 17 ( ( -LRB- cord-270326-fzsczb8m 105 18 5 5 CD cord-270326-fzsczb8m 105 19 - - SYM cord-270326-fzsczb8m 105 20 10 10 CD cord-270326-fzsczb8m 105 21 × × NNP cord-270326-fzsczb8m 105 22 10 10 CD cord-270326-fzsczb8m 105 23 ' ' NNP cord-270326-fzsczb8m 105 24 M M NNP cord-270326-fzsczb8m 105 25 ) ) -RRB- cord-270326-fzsczb8m 106 1 ( ( -LRB- cord-270326-fzsczb8m 106 2 Finbloom Finbloom NNP cord-270326-fzsczb8m 106 3 et et FW cord-270326-fzsczb8m 106 4 al al NNP cord-270326-fzsczb8m 106 5 . . NNP cord-270326-fzsczb8m 106 6 , , , cord-270326-fzsczb8m 106 7 1985 1985 CD cord-270326-fzsczb8m 106 8 ) ) -RRB- cord-270326-fzsczb8m 106 9 . . . cord-270326-fzsczb8m 107 1 The the DT cord-270326-fzsczb8m 107 2 number number NN cord-270326-fzsczb8m 107 3 of of IN cord-270326-fzsczb8m 107 4 binding bind VBG cord-270326-fzsczb8m 107 5 sites site NNS cord-270326-fzsczb8m 107 6 per per IN cord-270326-fzsczb8m 107 7 cell cell NN cord-270326-fzsczb8m 107 8 reported report VBN cord-270326-fzsczb8m 107 9 here here RB cord-270326-fzsczb8m 107 10 ( ( -LRB- cord-270326-fzsczb8m 107 11 11,100 11,100 CD cord-270326-fzsczb8m 107 12 sites site NNS cord-270326-fzsczb8m 107 13 / / SYM cord-270326-fzsczb8m 107 14 cell cell NN cord-270326-fzsczb8m 107 15 ) ) -RRB- cord-270326-fzsczb8m 107 16 is be VBZ cord-270326-fzsczb8m 107 17 similar similar JJ cord-270326-fzsczb8m 107 18 to to IN cord-270326-fzsczb8m 107 19 the the DT cord-270326-fzsczb8m 107 20 number number NN cord-270326-fzsczb8m 107 21 found find VBN cord-270326-fzsczb8m 107 22 for for IN cord-270326-fzsczb8m 107 23 murine murine JJ cord-270326-fzsczb8m 107 24 macrophages macrophage NNS cord-270326-fzsczb8m 107 25 ( ( -LRB- cord-270326-fzsczb8m 107 26 -~ -~ RB cord-270326-fzsczb8m 107 27 12,000 12,000 CD cord-270326-fzsczb8m 107 28 ) ) -RRB- cord-270326-fzsczb8m 107 29 ( ( -LRB- cord-270326-fzsczb8m 107 30 Celada Celada NNP cord-270326-fzsczb8m 107 31 et et NNP cord-270326-fzsczb8m 107 32 al al NNP cord-270326-fzsczb8m 107 33 . . NNP cord-270326-fzsczb8m 107 34 , , , cord-270326-fzsczb8m 107 35 1984 1984 CD cord-270326-fzsczb8m 107 36 ) ) -RRB- cord-270326-fzsczb8m 107 37 and and CC cord-270326-fzsczb8m 107 38 human human JJ cord-270326-fzsczb8m 107 39 fibroblasts fibroblast NNS cord-270326-fzsczb8m 107 40 ( ( -LRB- cord-270326-fzsczb8m 107 41 Anderson Anderson NNP cord-270326-fzsczb8m 107 42 et et NNP cord-270326-fzsczb8m 107 43 al al NNP cord-270326-fzsczb8m 107 44 . . NNP cord-270326-fzsczb8m 107 45 , , , cord-270326-fzsczb8m 107 46 1982 1982 CD cord-270326-fzsczb8m 107 47 ) ) -RRB- cord-270326-fzsczb8m 107 48 and and CC cord-270326-fzsczb8m 108 1 somewhat somewhat RB cord-270326-fzsczb8m 108 2 higher high JJR cord-270326-fzsczb8m 108 3 than than IN cord-270326-fzsczb8m 108 4 that that DT cord-270326-fzsczb8m 108 5 observed observe VBN cord-270326-fzsczb8m 108 6 on on IN cord-270326-fzsczb8m 108 7 human human JJ cord-270326-fzsczb8m 108 8 monocytes monocyte NNS cord-270326-fzsczb8m 108 9 ( ( -LRB- cord-270326-fzsczb8m 108 10 4000 4000 CD cord-270326-fzsczb8m 108 11 sites site NNS cord-270326-fzsczb8m 108 12 / / SYM cord-270326-fzsczb8m 108 13 cell cell NN cord-270326-fzsczb8m 108 14 ) ) -RRB- cord-270326-fzsczb8m 109 1 ( ( -LRB- cord-270326-fzsczb8m 109 2 Finbloom Finbloom NNP cord-270326-fzsczb8m 109 3 et et FW cord-270326-fzsczb8m 109 4 al al NNP cord-270326-fzsczb8m 109 5 . . NNP cord-270326-fzsczb8m 109 6 , , , cord-270326-fzsczb8m 109 7 1985 1985 CD cord-270326-fzsczb8m 109 8 ) ) -RRB- cord-270326-fzsczb8m 109 9 . . . cord-270326-fzsczb8m 110 1 The the DT cord-270326-fzsczb8m 110 2 binding binding NN cord-270326-fzsczb8m 110 3 is be VBZ cord-270326-fzsczb8m 110 4 specific specific JJ cord-270326-fzsczb8m 110 5 and and CC cord-270326-fzsczb8m 110 6 saturable saturable JJ cord-270326-fzsczb8m 111 1 ( ( -LRB- cord-270326-fzsczb8m 111 2 Fig Fig NNP cord-270326-fzsczb8m 111 3 . . NNP cord-270326-fzsczb8m 111 4 2 2 CD cord-270326-fzsczb8m 111 5 ) ) -RRB- cord-270326-fzsczb8m 111 6 . . . cord-270326-fzsczb8m 112 1 It -PRON- PRP cord-270326-fzsczb8m 112 2 is be VBZ cord-270326-fzsczb8m 112 3 also also RB cord-270326-fzsczb8m 112 4 species species NN cord-270326-fzsczb8m 112 5 - - HYPH cord-270326-fzsczb8m 112 6 specific specific JJ cord-270326-fzsczb8m 112 7 , , , cord-270326-fzsczb8m 112 8 as as IN cord-270326-fzsczb8m 112 9 Wistar Wistar NNP cord-270326-fzsczb8m 112 10 rat rat NN cord-270326-fzsczb8m 112 11 astrocytes astrocyte NNS cord-270326-fzsczb8m 112 12 and and CC cord-270326-fzsczb8m 112 13 the the DT cord-270326-fzsczb8m 112 14 rat rat NN cord-270326-fzsczb8m 112 15 astrocytoma astrocytoma NN cord-270326-fzsczb8m 112 16 cell cell NN cord-270326-fzsczb8m 112 17 line line NN cord-270326-fzsczb8m 112 18 C-6 C-6 NNP cord-270326-fzsczb8m 112 19 do do VBP cord-270326-fzsczb8m 112 20 not not RB cord-270326-fzsczb8m 112 21 bind bind VB cord-270326-fzsczb8m 112 22 murine murine JJ cord-270326-fzsczb8m 112 23 IFN IFN NNP cord-270326-fzsczb8m 112 24 - - HYPH cord-270326-fzsczb8m 112 25 y y NNP cord-270326-fzsczb8m 112 26 , , , cord-270326-fzsczb8m 112 27 maintaining maintain VBG cord-270326-fzsczb8m 112 28 the the DT cord-270326-fzsczb8m 112 29 species specie NNS cord-270326-fzsczb8m 112 30 specificity specificity NN cord-270326-fzsczb8m 112 31 found find VBN cord-270326-fzsczb8m 112 32 in in IN cord-270326-fzsczb8m 112 33 macrophages macrophage NNS cord-270326-fzsczb8m 112 34 . . . cord-270326-fzsczb8m 113 1 There there EX cord-270326-fzsczb8m 113 2 is be VBZ cord-270326-fzsczb8m 113 3 a a DT cord-270326-fzsczb8m 113 4 somewhat somewhat RB cord-270326-fzsczb8m 113 5 paradoxical paradoxical JJ cord-270326-fzsczb8m 113 6 situation situation NN cord-270326-fzsczb8m 113 7 concerning concern VBG cord-270326-fzsczb8m 113 8 the the DT cord-270326-fzsczb8m 113 9 apparent apparent JJ cord-270326-fzsczb8m 113 10 lack lack NN cord-270326-fzsczb8m 113 11 of of IN cord-270326-fzsczb8m 113 12 specific specific JJ cord-270326-fzsczb8m 113 13 binding binding NN cord-270326-fzsczb8m 113 14 of of IN cord-270326-fzsczb8m 113 15 mouse mouse NN cord-270326-fzsczb8m 113 16 IFN IFN NNP cord-270326-fzsczb8m 113 17 - - HYPH cord-270326-fzsczb8m 113 18 y y NNP cord-270326-fzsczb8m 113 19 to to IN cord-270326-fzsczb8m 113 20 rat rat NN cord-270326-fzsczb8m 113 21 cells cell NNS cord-270326-fzsczb8m 113 22 and and CC cord-270326-fzsczb8m 113 23 the the DT cord-270326-fzsczb8m 113 24 biological biological JJ cord-270326-fzsczb8m 113 25 effects effect NNS cord-270326-fzsczb8m 113 26 detected detect VBN cord-270326-fzsczb8m 113 27 in in IN cord-270326-fzsczb8m 113 28 rat rat NN cord-270326-fzsczb8m 113 29 astrocyte astrocyte NNP cord-270326-fzsczb8m 113 30 cultures culture NNS cord-270326-fzsczb8m 114 1 ( ( -LRB- cord-270326-fzsczb8m 114 2 Fiertz Fiertz NNP cord-270326-fzsczb8m 114 3 et et FW cord-270326-fzsczb8m 114 4 al al NNP cord-270326-fzsczb8m 114 5 . . NNP cord-270326-fzsczb8m 114 6 , , , cord-270326-fzsczb8m 114 7 1985 1985 CD cord-270326-fzsczb8m 114 8 ) ) -RRB- cord-270326-fzsczb8m 114 9 . . . cord-270326-fzsczb8m 115 1 This this DT cord-270326-fzsczb8m 115 2 could could MD cord-270326-fzsczb8m 115 3 be be VB cord-270326-fzsczb8m 115 4 explained explain VBN cord-270326-fzsczb8m 115 5 by by IN cord-270326-fzsczb8m 115 6 the the DT cord-270326-fzsczb8m 115 7 presence presence NN cord-270326-fzsczb8m 115 8 of of IN cord-270326-fzsczb8m 115 9 a a DT cord-270326-fzsczb8m 115 10 low low JJ cord-270326-fzsczb8m 115 11 affinity affinity NN cord-270326-fzsczb8m 115 12 receptor receptor NN cord-270326-fzsczb8m 115 13 only only RB cord-270326-fzsczb8m 115 14 noticeable noticeable JJ cord-270326-fzsczb8m 115 15 after after IN cord-270326-fzsczb8m 115 16 very very RB cord-270326-fzsczb8m 115 17 long long JJ cord-270326-fzsczb8m 115 18 periods period NNS cord-270326-fzsczb8m 115 19 of of IN cord-270326-fzsczb8m 115 20 incubation incubation NN cord-270326-fzsczb8m 115 21 ( ( -LRB- cord-270326-fzsczb8m 115 22 Celada Celada NNP cord-270326-fzsczb8m 115 23 et et NNP cord-270326-fzsczb8m 115 24 al al NNP cord-270326-fzsczb8m 115 25 . . NNP cord-270326-fzsczb8m 115 26 , , , cord-270326-fzsczb8m 115 27 1984 1984 CD cord-270326-fzsczb8m 115 28 ) ) -RRB- cord-270326-fzsczb8m 115 29 . . . cord-270326-fzsczb8m 116 1 The the DT cord-270326-fzsczb8m 116 2 human human JJ cord-270326-fzsczb8m 116 3 fibroblast fibroblast NN cord-270326-fzsczb8m 116 4 receptor receptor NN cord-270326-fzsczb8m 116 5 for for IN cord-270326-fzsczb8m 116 6 IFN IFN NNP cord-270326-fzsczb8m 116 7 - - HYPH cord-270326-fzsczb8m 116 8 y y NNP cord-270326-fzsczb8m 116 9 has have VBZ cord-270326-fzsczb8m 116 10 been be VBN cord-270326-fzsczb8m 116 11 reported report VBN cord-270326-fzsczb8m 116 12 to to TO cord-270326-fzsczb8m 116 13 be be VB cord-270326-fzsczb8m 116 14 internalized internalize VBN cord-270326-fzsczb8m 116 15 at at IN cord-270326-fzsczb8m 116 16 37 37 CD cord-270326-fzsczb8m 116 17 ° ° NN cord-270326-fzsczb8m 116 18 C C NNP cord-270326-fzsczb8m 116 19 ( ( -LRB- cord-270326-fzsczb8m 116 20 Anderson Anderson NNP cord-270326-fzsczb8m 116 21 et et NNP cord-270326-fzsczb8m 116 22 al al NNP cord-270326-fzsczb8m 116 23 . . NNP cord-270326-fzsczb8m 116 24 , , , cord-270326-fzsczb8m 116 25 1983 1983 CD cord-270326-fzsczb8m 116 26 ) ) -RRB- cord-270326-fzsczb8m 117 1 , , , cord-270326-fzsczb8m 117 2 We -PRON- PRP cord-270326-fzsczb8m 117 3 have have VBP cord-270326-fzsczb8m 117 4 observed observe VBN cord-270326-fzsczb8m 117 5 the the DT cord-270326-fzsczb8m 117 6 same same JJ cord-270326-fzsczb8m 117 7 phenomenon phenomenon NN cord-270326-fzsczb8m 117 8 in in IN cord-270326-fzsczb8m 117 9 an an DT cord-270326-fzsczb8m 117 10 immune immune JJ cord-270326-fzsczb8m 117 11 assay assay NN cord-270326-fzsczb8m 117 12 set set VBN cord-270326-fzsczb8m 117 13 up up RP cord-270326-fzsczb8m 117 14 with with IN cord-270326-fzsczb8m 117 15 specific specific JJ cord-270326-fzsczb8m 117 16 antibodies antibody NNS cord-270326-fzsczb8m 117 17 and and CC cord-270326-fzsczb8m 117 18 protein protein NN cord-270326-fzsczb8m 117 19 A a DT cord-270326-fzsczb8m 117 20 labeled label VBN cord-270326-fzsczb8m 117 21 with with IN cord-270326-fzsczb8m 117 22 ~-~-sI. ~-~-si. ADD cord-270326-fzsczb8m 118 1 The the DT cord-270326-fzsczb8m 118 2 endocytosis endocytosis NN cord-270326-fzsczb8m 118 3 of of IN cord-270326-fzsczb8m 118 4 IFN IFN NNP cord-270326-fzsczb8m 118 5 - - HYPH cord-270326-fzsczb8m 118 6 y y NNP cord-270326-fzsczb8m 118 7 bound bind VBN cord-270326-fzsczb8m 118 8 to to IN cord-270326-fzsczb8m 118 9 the the DT cord-270326-fzsczb8m 118 10 cell cell NN cord-270326-fzsczb8m 118 11 surface surface NN cord-270326-fzsczb8m 118 12 receptor receptor NN cord-270326-fzsczb8m 118 13 takes take VBZ cord-270326-fzsczb8m 118 14 place place NN cord-270326-fzsczb8m 118 15 at at IN cord-270326-fzsczb8m 118 16 37 37 CD cord-270326-fzsczb8m 118 17 ° ° NN cord-270326-fzsczb8m 118 18 C c NN cord-270326-fzsczb8m 118 19 but but CC cord-270326-fzsczb8m 118 20 not not RB cord-270326-fzsczb8m 118 21 at at IN cord-270326-fzsczb8m 118 22 4 4 CD cord-270326-fzsczb8m 118 23 ° ° NN cord-270326-fzsczb8m 118 24 C c NN cord-270326-fzsczb8m 118 25 and and CC cord-270326-fzsczb8m 118 26 a a DT cord-270326-fzsczb8m 118 27 rich rich JJ cord-270326-fzsczb8m 118 28 culture culture NN cord-270326-fzsczb8m 118 29 medium medium NN cord-270326-fzsczb8m 118 30 ( ( -LRB- cord-270326-fzsczb8m 118 31 DMEM DMEM NNP cord-270326-fzsczb8m 118 32 + + CC cord-270326-fzsczb8m 118 33 10 10 CD cord-270326-fzsczb8m 118 34 % % NN cord-270326-fzsczb8m 118 35 FCS FCS NNP cord-270326-fzsczb8m 118 36 ) ) -RRB- cord-270326-fzsczb8m 118 37 is be VBZ cord-270326-fzsczb8m 118 38 required require VBN cord-270326-fzsczb8m 118 39 , , , cord-270326-fzsczb8m 118 40 probably probably RB cord-270326-fzsczb8m 118 41 to to TO cord-270326-fzsczb8m 118 42 maintain maintain VB cord-270326-fzsczb8m 118 43 an an DT cord-270326-fzsczb8m 118 44 active active JJ cord-270326-fzsczb8m 118 45 metabolic metabolic JJ cord-270326-fzsczb8m 118 46 state state NN cord-270326-fzsczb8m 118 47 in in IN cord-270326-fzsczb8m 118 48 the the DT cord-270326-fzsczb8m 118 49 cultured cultured JJ cord-270326-fzsczb8m 118 50 astrocytes astrocyte NNS cord-270326-fzsczb8m 118 51 . . . cord-270326-fzsczb8m 119 1 Further further JJ cord-270326-fzsczb8m 119 2 demonstration demonstration NN cord-270326-fzsczb8m 119 3 of of IN cord-270326-fzsczb8m 119 4 internalization internalization NN cord-270326-fzsczb8m 119 5 by by IN cord-270326-fzsczb8m 119 6 an an DT cord-270326-fzsczb8m 119 7 energy energy NN cord-270326-fzsczb8m 119 8 - - HYPH cord-270326-fzsczb8m 119 9 dependent dependent JJ cord-270326-fzsczb8m 119 10 process process NN cord-270326-fzsczb8m 119 11 is be VBZ cord-270326-fzsczb8m 119 12 provided provide VBN cord-270326-fzsczb8m 119 13 for for IN cord-270326-fzsczb8m 119 14 the the DT cord-270326-fzsczb8m 119 15 direct direct JJ cord-270326-fzsczb8m 119 16 measure measure NN cord-270326-fzsczb8m 119 17 of of IN cord-270326-fzsczb8m 119 18 the the DT cord-270326-fzsczb8m 119 19 intracellular intracellular JJ cord-270326-fzsczb8m 119 20 labeled label VBN cord-270326-fzsczb8m 119 21 ligand ligand NN cord-270326-fzsczb8m 119 22 which which WDT cord-270326-fzsczb8m 119 23 takes take VBZ cord-270326-fzsczb8m 119 24 place place NN cord-270326-fzsczb8m 119 25 at at IN cord-270326-fzsczb8m 119 26 37 37 CD cord-270326-fzsczb8m 119 27 ° ° NN cord-270326-fzsczb8m 119 28 C c NN cord-270326-fzsczb8m 119 29 but but CC cord-270326-fzsczb8m 119 30 not not RB cord-270326-fzsczb8m 119 31 at at IN cord-270326-fzsczb8m 119 32 4 4 CD cord-270326-fzsczb8m 119 33 ° ° NN cord-270326-fzsczb8m 119 34 C c NN cord-270326-fzsczb8m 119 35 . . . cord-270326-fzsczb8m 120 1 High high JJ cord-270326-fzsczb8m 120 2 affinity affinity NN cord-270326-fzsczb8m 120 3 binding binding NN cord-270326-fzsczb8m 120 4 of of IN cord-270326-fzsczb8m 120 5 l-'5l l-'5l CD cord-270326-fzsczb8m 120 6 - - HYPH cord-270326-fzsczb8m 120 7 labelled label VBN cord-270326-fzsczb8m 120 8 mouse mouse NN cord-270326-fzsczb8m 120 9 interferon interferon NN cord-270326-fzsczb8m 120 10 to to IN cord-270326-fzsczb8m 120 11 a a DT cord-270326-fzsczb8m 120 12 specific specific JJ cord-270326-fzsczb8m 120 13 cell cell NN cord-270326-fzsczb8m 120 14 surface surface NN cord-270326-fzsczb8m 120 15 receptor receptor NN cord-270326-fzsczb8m 120 16 Molecular molecular JJ cord-270326-fzsczb8m 120 17 cloning cloning NN cord-270326-fzsczb8m 120 18 and and CC cord-270326-fzsczb8m 120 19 expression expression NN cord-270326-fzsczb8m 120 20 of of IN cord-270326-fzsczb8m 120 21 the the DT cord-270326-fzsczb8m 120 22 human human JJ cord-270326-fzsczb8m 120 23 interferon interferon NNP cord-270326-fzsczb8m 120 24 - - HYPH cord-270326-fzsczb8m 120 25 y y NNP cord-270326-fzsczb8m 120 26 receptor receptor NN cord-270326-fzsczb8m 120 27 Specific specific JJ cord-270326-fzsczb8m 120 28 binding binding NN cord-270326-fzsczb8m 120 29 of of IN cord-270326-fzsczb8m 120 30 1251-human 1251-human CD cord-270326-fzsczb8m 120 31 interferon interferon NNP cord-270326-fzsczb8m 120 32 - - HYPH cord-270326-fzsczb8m 120 33 y y NNP cord-270326-fzsczb8m 120 34 to to IN cord-270326-fzsczb8m 120 35 high high JJ cord-270326-fzsczb8m 120 36 affinity affinity NN cord-270326-fzsczb8m 120 37 receptors receptor NNS cord-270326-fzsczb8m 120 38 on on IN cord-270326-fzsczb8m 120 39 human human JJ cord-270326-fzsczb8m 120 40 fibroblasts fibroblast NNS cord-270326-fzsczb8m 121 1 Human human JJ cord-270326-fzsczb8m 121 2 interferon interferon NNP cord-270326-fzsczb8m 121 3 - - HYPH cord-270326-fzsczb8m 121 4 y y NNP cord-270326-fzsczb8m 121 5 is be VBZ cord-270326-fzsczb8m 121 6 internalized internalize VBN cord-270326-fzsczb8m 121 7 and and CC cord-270326-fzsczb8m 121 8 degraded degrade VBN cord-270326-fzsczb8m 121 9 by by IN cord-270326-fzsczb8m 121 10 cultured cultured JJ cord-270326-fzsczb8m 121 11 fibroblasts fibroblast NNS cord-270326-fzsczb8m 121 12 Evidence evidence NN cord-270326-fzsczb8m 121 13 that that WDT cord-270326-fzsczb8m 121 14 type type NN cord-270326-fzsczb8m 121 15 I -PRON- PRP cord-270326-fzsczb8m 121 16 and and CC cord-270326-fzsczb8m 121 17 II II NNP cord-270326-fzsczb8m 121 18 interferons interferon NNS cord-270326-fzsczb8m 121 19 have have VBP cord-270326-fzsczb8m 121 20 different different JJ cord-270326-fzsczb8m 121 21 receptors receptor NNS cord-270326-fzsczb8m 121 22 Evidence evidence NN cord-270326-fzsczb8m 121 23 for for IN cord-270326-fzsczb8m 121 24 a a DT cord-270326-fzsczb8m 121 25 gamma gamma NN cord-270326-fzsczb8m 121 26 interferon interferon NN cord-270326-fzsczb8m 121 27 receptor receptor NN cord-270326-fzsczb8m 121 28 that that WDT cord-270326-fzsczb8m 121 29 regulates regulate VBZ cord-270326-fzsczb8m 121 30 macrophage macrophage NN cord-270326-fzsczb8m 121 31 tumoricidal tumoricidal JJ cord-270326-fzsczb8m 121 32 activity activity NN cord-270326-fzsczb8m 121 33 Prolactin prolactin NN cord-270326-fzsczb8m 121 34 receptors receptor NNS cord-270326-fzsczb8m 121 35 in in IN cord-270326-fzsczb8m 121 36 cultured cultured JJ cord-270326-fzsczb8m 121 37 rat rat NN cord-270326-fzsczb8m 121 38 mammary mammary JJ cord-270326-fzsczb8m 121 39 tumor tumor NN cord-270326-fzsczb8m 121 40 cells cell NNS cord-270326-fzsczb8m 121 41 . . . cord-270326-fzsczb8m 122 1 Energy energy NN cord-270326-fzsczb8m 122 2 - - HYPH cord-270326-fzsczb8m 122 3 dependent dependent JJ cord-270326-fzsczb8m 122 4 uptake uptake NN cord-270326-fzsczb8m 122 5 and and CC cord-270326-fzsczb8m 122 6 degradation degradation NN cord-270326-fzsczb8m 122 7 of of IN cord-270326-fzsczb8m 122 8 hormone hormone NN cord-270326-fzsczb8m 122 9 receptors receptor NNS cord-270326-fzsczb8m 122 10 Monoclonal monoclonal JJ cord-270326-fzsczb8m 122 11 antibodies antibody NNS cord-270326-fzsczb8m 122 12 against against IN cord-270326-fzsczb8m 122 13 the the DT cord-270326-fzsczb8m 122 14 human human JJ cord-270326-fzsczb8m 122 15 interferon interferon NNP cord-270326-fzsczb8m 122 16 - - HYPH cord-270326-fzsczb8m 122 17 y y NNP cord-270326-fzsczb8m 122 18 receptor(s receptor(s NNP cord-270326-fzsczb8m 122 19 ) ) -RRB- cord-270326-fzsczb8m 122 20 . . . cord-270326-fzsczb8m 123 1 Mol Mol NNP cord-270326-fzsczb8m 123 2 . . . cord-270326-fzsczb8m 124 1 Immunol Immunol NNP cord-270326-fzsczb8m 124 2 Astrocytes Astrocytes NNP cord-270326-fzsczb8m 124 3 as as IN cord-270326-fzsczb8m 124 4 antigen antigen NN cord-270326-fzsczb8m 124 5 - - HYPH cord-270326-fzsczb8m 124 6 presenting present VBG cord-270326-fzsczb8m 124 7 cells cell NNS cord-270326-fzsczb8m 124 8 . . . cord-270326-fzsczb8m 125 1 I. I. NNP cord-270326-fzsczb8m 125 2 Induction Induction NNP cord-270326-fzsczb8m 125 3 of of IN cord-270326-fzsczb8m 125 4 la la NNP cord-270326-fzsczb8m 125 5 antigen antigen NN cord-270326-fzsczb8m 125 6 expression expression NN cord-270326-fzsczb8m 125 7 on on IN cord-270326-fzsczb8m 125 8 astrocytes astrocyte NNS cord-270326-fzsczb8m 125 9 by by IN cord-270326-fzsczb8m 125 10 T t NN cord-270326-fzsczb8m 125 11 cells cell NNS cord-270326-fzsczb8m 125 12 via via IN cord-270326-fzsczb8m 125 13 immune immune JJ cord-270326-fzsczb8m 125 14 interferon interferon NN cord-270326-fzsczb8m 125 15 and and CC cord-270326-fzsczb8m 125 16 its -PRON- PRP$ cord-270326-fzsczb8m 125 17 effect effect NN cord-270326-fzsczb8m 125 18 on on IN cord-270326-fzsczb8m 125 19 antigen antigen NN cord-270326-fzsczb8m 125 20 presentation presentation NN cord-270326-fzsczb8m 126 1 The the DT cord-270326-fzsczb8m 126 2 characteristics characteristic NNS cord-270326-fzsczb8m 126 3 of of IN cord-270326-fzsczb8m 126 4 binding bind VBG cord-270326-fzsczb8m 126 5 of of IN cord-270326-fzsczb8m 126 6 human human JJ cord-270326-fzsczb8m 126 7 recombinant recombinant JJ cord-270326-fzsczb8m 126 8 interferon interferon NNP cord-270326-fzsczb8m 126 9 - - HYPH cord-270326-fzsczb8m 126 10 y y NNP cord-270326-fzsczb8m 126 11 to to IN cord-270326-fzsczb8m 126 12 its -PRON- PRP$ cord-270326-fzsczb8m 126 13 receptor receptor NN cord-270326-fzsczb8m 126 14 on on IN cord-270326-fzsczb8m 126 15 human human JJ cord-270326-fzsczb8m 126 16 monocytes monocyte NNS cord-270326-fzsczb8m 126 17 and and CC cord-270326-fzsczb8m 126 18 human human JJ cord-270326-fzsczb8m 126 19 monocyte monocyte NNP cord-270326-fzsczb8m 126 20 - - HYPH cord-270326-fzsczb8m 126 21 like like JJ cord-270326-fzsczb8m 126 22 cell cell NN cord-270326-fzsczb8m 126 23 lines line NNS cord-270326-fzsczb8m 127 1 Astrocytes Astrocytes NNP cord-270326-fzsczb8m 127 2 present present VBP cord-270326-fzsczb8m 127 3 myelin myelin JJ cord-270326-fzsczb8m 127 4 basic basic JJ cord-270326-fzsczb8m 127 5 protein protein NN cord-270326-fzsczb8m 127 6 to to IN cord-270326-fzsczb8m 127 7 encephalitogenic encephalitogenic NNP cord-270326-fzsczb8m 127 8 T T NNP cord-270326-fzsczb8m 127 9 - - HYPH cord-270326-fzsczb8m 127 10 cell cell NN cord-270326-fzsczb8m 127 11 lines line NNS cord-270326-fzsczb8m 128 1 The the DT cord-270326-fzsczb8m 128 2 preparation preparation NN cord-270326-fzsczb8m 128 3 of of IN cord-270326-fzsczb8m 128 4 131I 131i CD cord-270326-fzsczb8m 128 5 - - HYPH cord-270326-fzsczb8m 128 6 labeled label VBN cord-270326-fzsczb8m 128 7 human human JJ cord-270326-fzsczb8m 128 8 growth growth NN cord-270326-fzsczb8m 128 9 hormone hormone NN cord-270326-fzsczb8m 128 10 of of IN cord-270326-fzsczb8m 128 11 high high JJ cord-270326-fzsczb8m 128 12 specific specific JJ cord-270326-fzsczb8m 128 13 radioactivity radioactivity NN cord-270326-fzsczb8m 128 14 Expression expression NN cord-270326-fzsczb8m 128 15 of of IN cord-270326-fzsczb8m 128 16 la la NN cord-270326-fzsczb8m 128 17 antigens antigen NNS cord-270326-fzsczb8m 128 18 by by IN cord-270326-fzsczb8m 128 19 cultured cultured JJ cord-270326-fzsczb8m 128 20 astrocytes astrocyte NNS cord-270326-fzsczb8m 128 21 treated treat VBN cord-270326-fzsczb8m 128 22 with with IN cord-270326-fzsczb8m 128 23 gamma gamma NN cord-270326-fzsczb8m 128 24 - - HYPH cord-270326-fzsczb8m 128 25 interferon interferon RB cord-270326-fzsczb8m 129 1 An an DT cord-270326-fzsczb8m 129 2 enzymic enzymic NN cord-270326-fzsczb8m 129 3 method method NN cord-270326-fzsczb8m 129 4 for for IN cord-270326-fzsczb8m 129 5 the the DT cord-270326-fzsczb8m 129 6 trace trace NN cord-270326-fzsczb8m 129 7 iodination iodination NN cord-270326-fzsczb8m 129 8 of of IN cord-270326-fzsczb8m 129 9 immunoglobulins immunoglobulin NNS cord-270326-fzsczb8m 129 10 and and CC cord-270326-fzsczb8m 129 11 other other JJ cord-270326-fzsczb8m 129 12 proteins protein NNS cord-270326-fzsczb8m 130 1 Viral viral JJ cord-270326-fzsczb8m 130 2 particles particle NNS cord-270326-fzsczb8m 130 3 induce induce VBP cord-270326-fzsczb8m 130 4 la la NNP cord-270326-fzsczb8m 130 5 antigen antigen NN cord-270326-fzsczb8m 130 6 expression expression NN cord-270326-fzsczb8m 130 7 on on IN cord-270326-fzsczb8m 130 8 astrocytes astrocyte NNS cord-270326-fzsczb8m 130 9 LIGAND ligand NN cord-270326-fzsczb8m 130 10 : : : cord-270326-fzsczb8m 130 11 a a DT cord-270326-fzsczb8m 130 12 versatile versatile JJ cord-270326-fzsczb8m 130 13 computerized computerized JJ cord-270326-fzsczb8m 130 14 approach approach NN cord-270326-fzsczb8m 130 15 for for IN cord-270326-fzsczb8m 130 16 characterization characterization NN cord-270326-fzsczb8m 130 17 of of IN cord-270326-fzsczb8m 130 18 ligandbinding ligandbinde VBG cord-270326-fzsczb8m 130 19 systems system NNS cord-270326-fzsczb8m 131 1 The the DT cord-270326-fzsczb8m 131 2 human human JJ cord-270326-fzsczb8m 131 3 interferon interferon NN cord-270326-fzsczb8m 131 4 - - HYPH cord-270326-fzsczb8m 131 5 y y NNP cord-270326-fzsczb8m 131 6 receptor receptor NN cord-270326-fzsczb8m 131 7 . . . cord-270326-fzsczb8m 132 1 Purification purification NN cord-270326-fzsczb8m 132 2 , , , cord-270326-fzsczb8m 132 3 characterization characterization NN cord-270326-fzsczb8m 132 4 and and CC cord-270326-fzsczb8m 132 5 preparation preparation NN cord-270326-fzsczb8m 132 6 of of IN cord-270326-fzsczb8m 132 7 antibodies antibody NNS cord-270326-fzsczb8m 132 8 Monoclonal monoclonal JJ cord-270326-fzsczb8m 132 9 antibodies antibody NNS cord-270326-fzsczb8m 132 10 to to IN cord-270326-fzsczb8m 132 11 the the DT cord-270326-fzsczb8m 132 12 human human JJ cord-270326-fzsczb8m 132 13 interferon interferon NNP cord-270326-fzsczb8m 132 14 - - HYPH cord-270326-fzsczb8m 132 15 y y NNP cord-270326-fzsczb8m 132 16 receptor receptor NN cord-270326-fzsczb8m 132 17 : : : cord-270326-fzsczb8m 133 1 blocking block VBG cord-270326-fzsczb8m 133 2 of of IN cord-270326-fzsczb8m 133 3 the the DT cord-270326-fzsczb8m 133 4 biological biological JJ cord-270326-fzsczb8m 133 5 activities activity NNS cord-270326-fzsczb8m 133 6 of of IN cord-270326-fzsczb8m 133 7 interferon interferon NN cord-270326-fzsczb8m 133 8 - - HYPH cord-270326-fzsczb8m 133 9 y y NN cord-270326-fzsczb8m 133 10 and and CC cord-270326-fzsczb8m 133 11 purification purification NN cord-270326-fzsczb8m 133 12 of of IN cord-270326-fzsczb8m 133 13 the the DT cord-270326-fzsczb8m 133 14 receptor receptor NN cord-270326-fzsczb8m 133 15 Immune Immune NNP cord-270326-fzsczb8m 133 16 response response NN cord-270326-fzsczb8m 133 17 gene gene NN cord-270326-fzsczb8m 133 18 products product NNS cord-270326-fzsczb8m 133 19 ( ( -LRB- cord-270326-fzsczb8m 133 20 Ia Ia NNP cord-270326-fzsczb8m 133 21 antigens antigen NNS cord-270326-fzsczb8m 133 22 ) ) -RRB- cord-270326-fzsczb8m 133 23 on on IN cord-270326-fzsczb8m 133 24 glial glial JJ cord-270326-fzsczb8m 133 25 and and CC cord-270326-fzsczb8m 133 26 endothelial endothelial JJ cord-270326-fzsczb8m 133 27 cells cell NNS cord-270326-fzsczb8m 133 28 in in IN cord-270326-fzsczb8m 133 29 virus virus NN cord-270326-fzsczb8m 133 30 induced induce VBN cord-270326-fzsczb8m 133 31 demyelination demyelination NN cord-270326-fzsczb8m 133 32 Lack lack NN cord-270326-fzsczb8m 133 33 of of IN cord-270326-fzsczb8m 133 34 cross cross JJ cord-270326-fzsczb8m 133 35 - - NN cord-270326-fzsczb8m 133 36 reaction reaction NN cord-270326-fzsczb8m 133 37 between between IN cord-270326-fzsczb8m 133 38 myelin myelin JJ cord-270326-fzsczb8m 133 39 basic basic JJ cord-270326-fzsczb8m 133 40 proteins protein NNS cord-270326-fzsczb8m 133 41 and and CC cord-270326-fzsczb8m 133 42 putative putative JJ cord-270326-fzsczb8m 133 43 demyelinating demyelinating JJ cord-270326-fzsczb8m 133 44 virus virus NN cord-270326-fzsczb8m 133 45 envelope envelope NN cord-270326-fzsczb8m 133 46 proteins protein NNS cord-270326-fzsczb8m 134 1 The the DT cord-270326-fzsczb8m 134 2 attraction attraction NN cord-270326-fzsczb8m 134 3 of of IN cord-270326-fzsczb8m 134 4 proteins protein NNS cord-270326-fzsczb8m 134 5 for for IN cord-270326-fzsczb8m 134 6 small small JJ cord-270326-fzsczb8m 134 7 molecules molecule NNS cord-270326-fzsczb8m 134 8 and and CC cord-270326-fzsczb8m 134 9 ions ion NNS cord-270326-fzsczb8m 134 10 Monoclonal monoclonal JJ cord-270326-fzsczb8m 134 11 antibodies antibody NNS cord-270326-fzsczb8m 134 12 to to IN cord-270326-fzsczb8m 134 13 murine murine JJ cord-270326-fzsczb8m 134 14 y y NN cord-270326-fzsczb8m 134 15 - - HYPH cord-270326-fzsczb8m 134 16 interferon interferon NNP cord-270326-fzsczb8m 134 17 which which WDT cord-270326-fzsczb8m 134 18 differentially differentially RB cord-270326-fzsczb8m 134 19 modulate modulate VBP cord-270326-fzsczb8m 134 20 activation activation NN cord-270326-fzsczb8m 134 21 and and CC cord-270326-fzsczb8m 134 22 antiviral antiviral JJ cord-270326-fzsczb8m 134 23 activity activity NN cord-270326-fzsczb8m 134 24 Regulation regulation NN cord-270326-fzsczb8m 134 25 of of IN cord-270326-fzsczb8m 134 26 murine murine JJ cord-270326-fzsczb8m 134 27 macrophage macrophage NN cord-270326-fzsczb8m 134 28 la la NNP cord-270326-fzsczb8m 134 29 antigen antigen NN cord-270326-fzsczb8m 134 30 expression expression NN cord-270326-fzsczb8m 134 31 by by IN cord-270326-fzsczb8m 134 32 lymphokine lymphokine NN cord-270326-fzsczb8m 134 33 with with IN cord-270326-fzsczb8m 134 34 immune immune JJ cord-270326-fzsczb8m 134 35 interferon interferon NN cord-270326-fzsczb8m 134 36 activity activity NN cord-270326-fzsczb8m 134 37 Ia Ia NNP cord-270326-fzsczb8m 134 38 - - HYPH cord-270326-fzsczb8m 134 39 restricted restrict VBN cord-270326-fzsczb8m 134 40 encephalitogenic encephalitogenic NNP cord-270326-fzsczb8m 134 41 T t NN cord-270326-fzsczb8m 134 42 lymphocytes lymphocyte NNS cord-270326-fzsczb8m 134 43 mediating mediate VBG cord-270326-fzsczb8m 134 44 EAE EAE NNP cord-270326-fzsczb8m 134 45 lyse lyse VBP cord-270326-fzsczb8m 134 46 autoantigen autoantigen NN cord-270326-fzsczb8m 134 47 - - HYPH cord-270326-fzsczb8m 134 48 presenting present VBG cord-270326-fzsczb8m 134 49 astrocytes astrocyte VBZ cord-270326-fzsczb8m 134 50 Coronavirus Coronavirus NNP cord-270326-fzsczb8m 134 51 infection infection NN cord-270326-fzsczb8m 134 52 induces induce VBZ cord-270326-fzsczb8m 134 53 H-2 H-2 NNP cord-270326-fzsczb8m 134 54 antigen antigen NN cord-270326-fzsczb8m 134 55 expression expression NN cord-270326-fzsczb8m 134 56 on on IN cord-270326-fzsczb8m 134 57 oligodendrocytes oligodendrocyte NNS cord-270326-fzsczb8m 134 58 and and CC cord-270326-fzsczb8m 134 59 astrocytes astrocyte NNS cord-270326-fzsczb8m 134 60 Astrocytes Astrocytes NNPS cord-270326-fzsczb8m 134 61 produce produce VBP cord-270326-fzsczb8m 134 62 interferon interferon NN cord-270326-fzsczb8m 134 63 that that WDT cord-270326-fzsczb8m 134 64 enhances enhance VBZ cord-270326-fzsczb8m 134 65 the the DT cord-270326-fzsczb8m 134 66 expression expression NN cord-270326-fzsczb8m 134 67 of of IN cord-270326-fzsczb8m 134 68 H-2 h-2 NN cord-270326-fzsczb8m 134 69 antigens antigen NNS cord-270326-fzsczb8m 134 70 on on IN cord-270326-fzsczb8m 134 71 a a DT cord-270326-fzsczb8m 134 72 subpopulation subpopulation NN cord-270326-fzsczb8m 134 73 of of IN cord-270326-fzsczb8m 134 74 brain brain NN cord-270326-fzsczb8m 134 75 cells cell NNS cord-270326-fzsczb8m 134 76 Immune immune VBP cord-270326-fzsczb8m 134 77 interferon interferon NN cord-270326-fzsczb8m 134 78 : : : cord-270326-fzsczb8m 134 79 a a DT cord-270326-fzsczb8m 134 80 pleiotropic pleiotropic JJ cord-270326-fzsczb8m 134 81 lymphokine lymphokine NN cord-270326-fzsczb8m 134 82 with with IN cord-270326-fzsczb8m 134 83 multiple multiple JJ cord-270326-fzsczb8m 134 84 effects effect NNS cord-270326-fzsczb8m 134 85 Distribution distribution NN cord-270326-fzsczb8m 134 86 and and CC cord-270326-fzsczb8m 134 87 quantitation quantitation NN cord-270326-fzsczb8m 134 88 of of IN cord-270326-fzsczb8m 134 89 HLA HLA NNP cord-270326-fzsczb8m 134 90 - - HYPH cord-270326-fzsczb8m 134 91 ABC ABC NNP cord-270326-fzsczb8m 134 92 and and CC cord-270326-fzsczb8m 134 93 DR DR NNP cord-270326-fzsczb8m 134 94 ( ( -LRB- cord-270326-fzsczb8m 134 95 Ia Ia NNP cord-270326-fzsczb8m 134 96 ) ) -RRB- cord-270326-fzsczb8m 134 97 antigens antigen NNS cord-270326-fzsczb8m 134 98 on on IN cord-270326-fzsczb8m 134 99 human human JJ cord-270326-fzsczb8m 134 100 kidney kidney NN cord-270326-fzsczb8m 134 101 and and CC cord-270326-fzsczb8m 134 102 other other JJ cord-270326-fzsczb8m 134 103 tissues tissue NNS cord-270326-fzsczb8m 135 1 Interferongamma Interferongamma NNP cord-270326-fzsczb8m 135 2 induces induce VBZ cord-270326-fzsczb8m 135 3 the the DT cord-270326-fzsczb8m 135 4 expression expression NN cord-270326-fzsczb8m 135 5 of of IN cord-270326-fzsczb8m 135 6 H-2 H-2 NNP cord-270326-fzsczb8m 135 7 and and CC cord-270326-fzsczb8m 135 8 la la NNP cord-270326-fzsczb8m 135 9 antigens antigen NNS cord-270326-fzsczb8m 135 10 on on IN cord-270326-fzsczb8m 135 11 brain brain NN cord-270326-fzsczb8m 135 12 cells cell NNS