id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001826-av2gxfxy	Gao, Qian	A cell-based high-throughput approach to identify inhibitors of influenza A virus	2014-07-14		.txt	text/plain	2829	165	48	In this study we established a 293T cell line that constitutively synthesizes a virus-based negative strand RNA, which expresses Gaussia luciferase upon influenza A virus infection. The dynamic signal range of the Gluc reporter assay was assessed by infecting 293T-Gluc cells with varying quantities of influenza A/WSN/33 virus (MOI of 0.0001, 0.001, 0.01, 0.1 and 1) and determining Gluc activity at various times post-infection (12, 24, 36 and 48 h post-infection). For evaluation assay of antivirals and high-throughput screening, 1 μL of each tested compound was added to cells and incubated for 2 h prior to infection, after which cells were infected with influenza A/WSN/33 virus at an MOI of 0.05. The above results suggest the potential to use the Gluc reporter assay to screen compounds against influenza A virus. In this work we reported a cell-based high-throughput assay to identify inhibitors for influenza virus.	./cache/cord-001826-av2gxfxy.txt	./txt/cord-001826-av2gxfxy.txt