id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005188-7syyl40y	Matsubayashi, Makoto	First report of molecular identification of Cystoisospora suis in piglets with lethal diarrhea in Japan	2016-04-01		.txt	text/plain	2706	163	49	title: First report of molecular identification of Cystoisospora suis in piglets with lethal diarrhea in Japan Cystoisospora suis is a pathogen that causes diarrhea in pigs and can lead to serious disease. In this study, we used histopathological, bacteriological, virological, and parasitological methods to identify the cause of the disease in two piglets with severe diarrhea. C. suis in particular leads to serious disease in suckling piglets, causing diarrhea and dehydration mainly in animals 2-4 weeks of age (Mundt et al. In this study, we used histopathological, bacteriological, virological, and genetic methods to identify the causes of disease in piglets with severe diarrhea. For virological examination, contents of the small intestines of the two piglets were inspected by species-specific PCR using PED and TGE viruses (Kim et al. By using Cystoisospora-specific PCR, the predicted 440-bp product of the ITS1 gene was successfully amplified in DNAs from the cryopreserved ileum and serial sections only of the No. 1 piglet.	./cache/cord-005188-7syyl40y.txt	./txt/cord-005188-7syyl40y.txt