id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004307-4sltubqk	Horiuchi, Sho	A novel loop-mediated isothermal amplification method for efficient and robust detection of EGFR mutations	2020-01-14		.txt	text/plain	2952	151	48	The activation of somatic mutations conferring sensitivity to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors has been widely used in the development of advanced or metastatic primary lung cancer therapy. In the present study, a loop-mediated isothermal amplification (LAMP) method was used to identify EGFR mutations, and its efficiency was compared with the Therascreen quantitative PCR assay. Following removal of normal lung tissues, DNA samples were extracted as aforementioned, and investigated using Therascreen EGFR PCR and a LAMP assay. The additional Case X experiments also identified a novel EGFR mutation using direct sequencing which was not identified using Therascreen or LAMP; however, this mutation may have been detected as an exon 19 deletion by the primers of the similarly targeted mutation. A rapid, sensitive assay to detect EGFR mutation in small biopsy specimens from lung cancer Epidermal growth factor receptor gene mutation in non-small cell lung cancer using highly sensitive and fast TaqMan PCR assay	./cache/cord-004307-4sltubqk.txt	./txt/cord-004307-4sltubqk.txt