id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-288887-lshsgex3	Yoda, Tomoko	Evaluation and application of reverse transcription loop‐mediated isothermal amplification for detection of noroviruses	2007-01-23		.txt	text/plain	4922	236	56	The loop-mediated isothermal amplification (LAMP) assay originally described [Notomi et al., 2000] is based on the principle of autocycling strand displacement DNA synthesis for the detection of a specific DNA sequence with specific characteristics: (1) all reactions can be conducted under isothermal conditions ranging from 60 to 658C; (2) the specificity of the reaction is extremely high because it uses six primers [Nagamine et al., 2002] recognizing eight distinct regions on the target nucleotides; and (3) a simple detection method such as visual judgment using Fluorescent Detection Reagent (Eiken Chemical Co., Ltd.) is possible. This study describes (a) the evaluation of a newly developed RT-LAMP assay for the detection of NVs and compares the results with that of the routine RT-PCR assay, (b) comparisons between the RT-LAMP assay and conventional RT-LAMP detection kits for NVs (Eiken Chemical Co., Ltd.), and (c) application of the RT-LAMP assay for outbreaks of acute gastroenteritis.	./cache/cord-288887-lshsgex3.txt	./txt/cord-288887-lshsgex3.txt