id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302663-gb2vgs97	Mekata, Tohru	Detection of yellow head virus in shrimp by loop-mediated isothermal amplification (LAMP)	2006-04-04		.txt	text/plain	2543	148	63	Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting the structural glycoprotein gene of yellow head virus (YHV). The RT-LAMP assay is a novel method of gene amplification that amplifies nucleic acid with high specificity, sensitivity and rapidity under isothermal conditions with a set of four specially designed primers that recognize six distinct sequences of the target. The development of a loop-mediated isothermal amplification (LAMP) assay for detection of white spot disease virus (WSDV) DNA was described by Kono et al. Ten-fold serial dilutions (10 −1 to 10 −8 diluted) of RNA extracted from YHV-infected shrimp was used as a template for RT-LAMP according to determined conditions. In order to determine the sensitivity of detection limit, RT-LAMP and nested RT-PCR were carried out using various concentrations (10 −1 to 10 −8 dilution) of RNA extracted from YHV-infected shrimp as template.	./cache/cord-302663-gb2vgs97.txt	./txt/cord-302663-gb2vgs97.txt