id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316816-yjbcvf3o	Cardoso, Tereza C.	Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye	2010-08-21		.txt	text/plain	2077	104	51	title: Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid visual detection of turkey coronavirus (TCoV) infection. In conclusion, RT-LAMP with HNB dye was shown to be a sensitive, simple assay for the rapid diagnosis of TCoV infection, either directly from feces or in association with virus isolation methods. The objective of this study was to develop and validate a reversetranscription loop-mediated isothermal amplification (LAMP) method for the direct detection of viral RNA from tissues and feces collected from experimentally and naturally infected birds. Embryo tissues (ileum and ileumecaecal junction portions) were prepared by infecting 25-day-old embryonated turkey eggs with 0.3 ml 10 2.4 EID 50 TCoV via the amniotic sac, as described previously [9, 10] . Reverse transcription loop-mediated isothermal amplification for rapid detection of infectious bronchitis virus in infected chicken tissues	./cache/cord-316816-yjbcvf3o.txt	./txt/cord-316816-yjbcvf3o.txt