id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332820-6qx6svs5	Buck, M. D.	Standard operating procedures for SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay	2020-07-01		.txt	text/plain	4095	252	52	The pipeline utilises a series of in-house buffers to first inactivate patient samples received from care homes and hospitals, and to then extract RNA before using a CE marked commercial kit to detect SARS-CoV-2 by RT-qPCR. Herein, we describe the use of loop mediated isothermal amplification PCR coupled with reverse transcription (RT-LAMP) as a robust method for SARS-CoV-2 detection in clinical specimens 6 . Our results demonstrate that within the CCC pipeline, RT-LAMP can readily replace RT-qPCR as a means for detecting SARS-CoV-2 transcripts within RNA extracted from nosethroat swabs and endotracheal secretions/bronchoalveolar lavage fluid. The RT-LAMP assay reproducibility and precision were determined by extracting RNA 5 times from a confirmed COVID-19 positive patient sample through the CCC pipeline and assessing by N gene and 18S RT-LAMP in 5 independent experiments, performed by two different operators ( Figure 4D ).	./cache/cord-332820-6qx6svs5.txt	./txt/cord-332820-6qx6svs5.txt