id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332961-ebr623rm	Zhang, Qingli	Reverse transcription loop-mediated isothermal amplification for rapid and quantitative assay of covert mortality nodavirus in shrimp	2017-11-30		.txt	text/plain	3900	175	47	In this study, we report a sensitive and specific real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid and quantitative detection of CMNV. In this study, we developed a rapid and sensitive reverse transcription loopmediated isothermal amplification (RT-LAMP) method for the detection of CMNV in infected shrimp. For CMNV quantitative detection of samples, a standard curve was generated for CMNV qRT-LAMP by plotting a graph between different concentrations of pMD19-T-CMNV plasmids, ranging from 10 8 to 10 0 copy numbers, to cycle threshold (Ct) values, obtained through real-time monitoring of the amplification. The lowest detection limit of the newly developed CMNV RT-LAMP method was 6.2 pg of total RNA when the reactions were tested using 1 lL of 10-fold serially diluted RNA from shrimp artificially infected with CMNV (Fig. 3A) . Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing	./cache/cord-332961-ebr623rm.txt	./txt/cord-332961-ebr623rm.txt