id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-334518-mjr6u7ak	Hu, X.	Development and clinical application of a rapid and sensitive loop-mediated isothermalamplification test for SARS-CoV-2 infection	2020-05-23		.txt	text/plain	5158	294	51	To accelerate clinical diagnostic testing for COVID-19, we conducted a prospective cohort study to develop and validate a novel RT-LAMP assay capable of detecting SARS-CoV-2 RNA for potential use in centralized facilities and point-of-care settings. Subsequently, we evaluated the RT-LAMP and standard RT-qPCR assays on 329 nasopharyngeal swabs from a cohort of 129 suspected COVID-19 patients and on the serial upper respiratory samples from an asymptomatic carrier, and the insistent samples between RT-LAMP and RT-qPCR were further subjected to next-generation sequencing (NGS) for SARS-CoV-2 confirmation. . https://doi.org/10.1101/2020.05.20.20108530 doi: medRxiv preprint As described in the Materials and Methods, we developed a rapid and simple RT-LAMP assay to detect SARS-CoV-2 RNA, and positive reactions resulted in a color change from purple to blue due to decreased magnesium concentration in the presence of extensive Bst DNA polymerase activity, while negative reactions retained the purple color.	./cache/cord-334518-mjr6u7ak.txt	./txt/cord-334518-mjr6u7ak.txt