id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-343136-kftffes0	Mohon, Abu Naser	Optimization and clinical validation of dual-target RT-LAMP for SARS-CoV-2	2020-09-15		.txt	text/plain	2591	164	54	A novel reverse-transcriptase loop mediated amplification (RT-LAMP) method targeting genes encoding the Spike (S) protein and RNA-dependent RNA polymerase (RdRP) of SARS-CoV-2 has been developed. Limit of detection of the LAMP assay was evaluated by using a nasopharyngeal (NP) swab sample infected with SARS-CoV-2 for which the viral load was quantified using digital droplet PCR (see Supplementary Methods). Twenty four replicates from a serial dilution containing 25-50 copies of SARS-CoV-2 which equates to 1X LOD (patient sample NP swab in VTM viral load confirmed by digital droplet PCR) per reaction were tested using dual-target RT-LAMP (Table 3) . The dual-target RT-LAMP test for SARS-CoV-2 developed in this study has comparable analytical sensitivity and specificity, limit of detection, precision, and achieved excellent agreement compared to the reference RT-PCR methods used internationally.	./cache/cord-343136-kftffes0.txt	./txt/cord-343136-kftffes0.txt