id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-344636-go5cw92q	Huang, Wei E.	RT‐LAMP for rapid diagnosis of coronavirus SARS‐CoV‐2	2020-04-25		.txt	text/plain	4771	253	61	In this work, we developed a COVID-19 diagnosis kit for the rapid detection of SARS-CoV-2, using one-step reverse transcription and loop-mediated isothermal amplification (RT-LAMP). Positive amplification products were obtained even for 2 copies of the synthetic viral DNA fragment template in 30 min when using the S17 primers (lane 4 in Fig. 1D ), demonstrating that the LAMP reaction was rapid and sensitive. To assess the potential of RT-LAMP in detecting RNA virus of SARS-CoV-2, we then tested the performance of these primers with synthesized RNA fragments of the N gene, S gene and Orf1ab gene obtained from in vitro transcription (Appendix S1). The ultimate aim is to develop an enclosed device that integrates RNA extraction, purification, reverse transcription (RT) and loop-mediated isothermal amplification (LAMP) to detect the SARS-CoV-2 virus directly from a throat swab sample.	./cache/cord-344636-go5cw92q.txt	./txt/cord-344636-go5cw92q.txt