id sid tid token lemma pos cord-285088-krim73zt 1 1 key key NN cord-285088-krim73zt 1 2 : : : cord-285088-krim73zt 1 3 cord-285088-krim73zt cord-285088-krim73zt CD cord-285088-krim73zt 1 4 authors author NNS cord-285088-krim73zt 1 5 : : : cord-285088-krim73zt 1 6 Wang Wang NNP cord-285088-krim73zt 1 7 , , , cord-285088-krim73zt 1 8 Deguo Deguo NNP cord-285088-krim73zt 1 9 title title NN cord-285088-krim73zt 1 10 : : : cord-285088-krim73zt 1 11 One one CD cord-285088-krim73zt 1 12 - - HYPH cord-285088-krim73zt 1 13 pot pot NN cord-285088-krim73zt 1 14 Detection detection NN cord-285088-krim73zt 1 15 of of IN cord-285088-krim73zt 1 16 COVID-19 COVID-19 NNP cord-285088-krim73zt 1 17 with with IN cord-285088-krim73zt 1 18 Real real JJ cord-285088-krim73zt 1 19 - - HYPH cord-285088-krim73zt 1 20 time time NN cord-285088-krim73zt 1 21 Reverse reverse NN cord-285088-krim73zt 1 22 - - HYPH cord-285088-krim73zt 1 23 transcription transcription NN cord-285088-krim73zt 1 24 Loop loop NN cord-285088-krim73zt 1 25 - - HYPH cord-285088-krim73zt 1 26 mediated mediate VBN cord-285088-krim73zt 1 27 Isothermal Isothermal NNP cord-285088-krim73zt 1 28 Amplification Amplification NNP cord-285088-krim73zt 1 29 ( ( -LRB- cord-285088-krim73zt 1 30 RT RT NNP cord-285088-krim73zt 1 31 - - HYPH cord-285088-krim73zt 1 32 LAMP LAMP NNP cord-285088-krim73zt 1 33 ) ) -RRB- cord-285088-krim73zt 1 34 Assay Assay NNP cord-285088-krim73zt 1 35 and and CC cord-285088-krim73zt 1 36 Visual visual JJ cord-285088-krim73zt 1 37 RT RT NNP cord-285088-krim73zt 1 38 - - HYPH cord-285088-krim73zt 1 39 LAMP LAMP NNP cord-285088-krim73zt 1 40 Assay Assay NNP cord-285088-krim73zt 1 41 date date NN cord-285088-krim73zt 1 42 : : : cord-285088-krim73zt 1 43 2020 2020 CD cord-285088-krim73zt 1 44 - - HYPH cord-285088-krim73zt 1 45 04 04 CD cord-285088-krim73zt 1 46 - - SYM cord-285088-krim73zt 1 47 21 21 CD cord-285088-krim73zt 1 48 journal journal NN cord-285088-krim73zt 1 49 : : : cord-285088-krim73zt 2 1 bioRxiv biorxiv IN cord-285088-krim73zt 2 2 DOI DOI NNP cord-285088-krim73zt 2 3 : : : cord-285088-krim73zt 3 1 10.1101/2020.04.21.052530 10.1101/2020.04.21.052530 CD cord-285088-krim73zt 3 2 sha sha NNP cord-285088-krim73zt 3 3 : : : cord-285088-krim73zt 4 1 e1104082358cfdbf52a0e2307b7993fb249f4567 e1104082358cfdbf52a0e2307b7993fb249f4567 . cord-285088-krim73zt 5 1 doc_id doc_id CD cord-285088-krim73zt 5 2 : : : cord-285088-krim73zt 5 3 285088 285088 CD cord-285088-krim73zt 5 4 cord_uid cord_uid NNS cord-285088-krim73zt 5 5 : : : cord-285088-krim73zt 6 1 krim73zt krim73zt NNP cord-285088-krim73zt 6 2 Background Background NNP cord-285088-krim73zt 6 3 Rapid Rapid NNP cord-285088-krim73zt 6 4 and and CC cord-285088-krim73zt 6 5 reliable reliable JJ cord-285088-krim73zt 6 6 diagnostic diagnostic JJ cord-285088-krim73zt 6 7 assays assay NNS cord-285088-krim73zt 6 8 were be VBD cord-285088-krim73zt 6 9 critical critical JJ cord-285088-krim73zt 6 10 for for IN cord-285088-krim73zt 6 11 prevention prevention NN cord-285088-krim73zt 6 12 and and CC cord-285088-krim73zt 6 13 control control NN cord-285088-krim73zt 6 14 of of IN cord-285088-krim73zt 6 15 the the DT cord-285088-krim73zt 6 16 coronavirus coronavirus NN cord-285088-krim73zt 6 17 pneumonia pneumonia NN cord-285088-krim73zt 6 18 caused cause VBN cord-285088-krim73zt 6 19 by by IN cord-285088-krim73zt 6 20 COVID-19 COVID-19 NNP cord-285088-krim73zt 6 21 . . . cord-285088-krim73zt 7 1 Objective objective JJ cord-285088-krim73zt 8 1 This this DT cord-285088-krim73zt 8 2 study study NN cord-285088-krim73zt 8 3 was be VBD cord-285088-krim73zt 8 4 to to TO cord-285088-krim73zt 8 5 establish establish VB cord-285088-krim73zt 8 6 one one CD cord-285088-krim73zt 8 7 - - HYPH cord-285088-krim73zt 8 8 pot pot NN cord-285088-krim73zt 8 9 real real JJ cord-285088-krim73zt 8 10 - - HYPH cord-285088-krim73zt 8 11 time time NN cord-285088-krim73zt 8 12 reverse reverse NN cord-285088-krim73zt 8 13 - - HYPH cord-285088-krim73zt 8 14 transcription transcription NN cord-285088-krim73zt 8 15 loop loop NN cord-285088-krim73zt 8 16 - - HYPH cord-285088-krim73zt 8 17 mediated mediate VBN cord-285088-krim73zt 8 18 isothermal isothermal JJ cord-285088-krim73zt 8 19 amplification amplification NN cord-285088-krim73zt 8 20 ( ( -LRB- cord-285088-krim73zt 8 21 RT RT NNP cord-285088-krim73zt 8 22 - - HYPH cord-285088-krim73zt 8 23 LAMP LAMP NNP cord-285088-krim73zt 8 24 ) ) -RRB- cord-285088-krim73zt 8 25 assay assay NN cord-285088-krim73zt 8 26 and and CC cord-285088-krim73zt 8 27 one one CD cord-285088-krim73zt 8 28 - - HYPH cord-285088-krim73zt 8 29 pot pot NN cord-285088-krim73zt 8 30 visual visual JJ cord-285088-krim73zt 8 31 RT rt NN cord-285088-krim73zt 8 32 - - HYPH cord-285088-krim73zt 8 33 LAMP LAMP NNP cord-285088-krim73zt 8 34 assay assay NN cord-285088-krim73zt 8 35 for for IN cord-285088-krim73zt 8 36 the the DT cord-285088-krim73zt 8 37 detection detection NN cord-285088-krim73zt 8 38 of of IN cord-285088-krim73zt 8 39 COVID-19 COVID-19 NNP cord-285088-krim73zt 8 40 . . . cord-285088-krim73zt 9 1 Methods method NNS cord-285088-krim73zt 9 2 Six six CD cord-285088-krim73zt 9 3 specific specific JJ cord-285088-krim73zt 9 4 LAMP lamp NN cord-285088-krim73zt 9 5 primers primer NNS cord-285088-krim73zt 9 6 targeting target VBG cord-285088-krim73zt 9 7 the the DT cord-285088-krim73zt 9 8 N n CD cord-285088-krim73zt 9 9 gene gene NN cord-285088-krim73zt 9 10 of of IN cord-285088-krim73zt 9 11 COVID-19 COVID-19 NNP cord-285088-krim73zt 9 12 were be VBD cord-285088-krim73zt 9 13 designed design VBN cord-285088-krim73zt 9 14 , , , cord-285088-krim73zt 9 15 the the DT cord-285088-krim73zt 9 16 RT RT NNP cord-285088-krim73zt 9 17 - - HYPH cord-285088-krim73zt 9 18 LAMP LAMP NNP cord-285088-krim73zt 9 19 reaction reaction NN cord-285088-krim73zt 9 20 system system NN cord-285088-krim73zt 9 21 was be VBD cord-285088-krim73zt 9 22 optimized optimize VBN cord-285088-krim73zt 9 23 with with IN cord-285088-krim73zt 9 24 plasmid plasmid NN cord-285088-krim73zt 9 25 pUC57 pUC57 NNP cord-285088-krim73zt 9 26 containing contain VBG cord-285088-krim73zt 9 27 N N NNP cord-285088-krim73zt 9 28 gene gene NN cord-285088-krim73zt 9 29 sequence sequence NN cord-285088-krim73zt 9 30 , , , cord-285088-krim73zt 9 31 the the DT cord-285088-krim73zt 9 32 detection detection NN cord-285088-krim73zt 9 33 limit limit NN cord-285088-krim73zt 9 34 was be VBD cord-285088-krim73zt 9 35 determined determine VBN cord-285088-krim73zt 9 36 with with IN cord-285088-krim73zt 9 37 a a DT cord-285088-krim73zt 9 38 serial serial JJ cord-285088-krim73zt 9 39 dilution dilution NN cord-285088-krim73zt 9 40 of of IN cord-285088-krim73zt 9 41 the the DT cord-285088-krim73zt 9 42 plasmid plasmid NN cord-285088-krim73zt 9 43 pUC57 puc57 NN cord-285088-krim73zt 9 44 containing contain VBG cord-285088-krim73zt 9 45 N N NNP cord-285088-krim73zt 9 46 gene gene NN cord-285088-krim73zt 9 47 sequence sequence NN cord-285088-krim73zt 9 48 , , , cord-285088-krim73zt 9 49 and and CC cord-285088-krim73zt 9 50 the the DT cord-285088-krim73zt 9 51 one one CD cord-285088-krim73zt 9 52 - - HYPH cord-285088-krim73zt 9 53 pot pot NN cord-285088-krim73zt 9 54 real real JJ cord-285088-krim73zt 9 55 - - HYPH cord-285088-krim73zt 9 56 time time NN cord-285088-krim73zt 9 57 RT RT NNP cord-285088-krim73zt 9 58 - - HYPH cord-285088-krim73zt 9 59 LAMP LAMP NNP cord-285088-krim73zt 9 60 assay assay NN cord-285088-krim73zt 9 61 and and CC cord-285088-krim73zt 9 62 one one CD cord-285088-krim73zt 9 63 - - HYPH cord-285088-krim73zt 9 64 pot pot NN cord-285088-krim73zt 9 65 visual visual JJ cord-285088-krim73zt 9 66 RT rt NN cord-285088-krim73zt 9 67 - - HYPH cord-285088-krim73zt 9 68 LAMP LAMP NNP cord-285088-krim73zt 9 69 assay assay NN cord-285088-krim73zt 9 70 for for IN cord-285088-krim73zt 9 71 the the DT cord-285088-krim73zt 9 72 detection detection NN cord-285088-krim73zt 9 73 of of IN cord-285088-krim73zt 9 74 COVID-19 COVID-19 NNP cord-285088-krim73zt 9 75 were be VBD cord-285088-krim73zt 9 76 established establish VBN cord-285088-krim73zt 9 77 . . . cord-285088-krim73zt 10 1 Results result NNS cord-285088-krim73zt 11 1 Our -PRON- PRP$ cord-285088-krim73zt 11 2 results result NNS cord-285088-krim73zt 11 3 showed show VBD cord-285088-krim73zt 11 4 that that IN cord-285088-krim73zt 11 5 the the DT cord-285088-krim73zt 11 6 one one CD cord-285088-krim73zt 11 7 - - HYPH cord-285088-krim73zt 11 8 pot pot NN cord-285088-krim73zt 11 9 RT rt NN cord-285088-krim73zt 11 10 - - HYPH cord-285088-krim73zt 11 11 LAMP LAMP NNP cord-285088-krim73zt 11 12 assays assay NNS cord-285088-krim73zt 11 13 can can MD cord-285088-krim73zt 11 14 detect detect VB cord-285088-krim73zt 11 15 COVID-19 COVID-19 NNP cord-285088-krim73zt 11 16 with with IN cord-285088-krim73zt 11 17 a a DT cord-285088-krim73zt 11 18 limit limit NN cord-285088-krim73zt 11 19 of of IN cord-285088-krim73zt 11 20 ≥ ≥ CD cord-285088-krim73zt 11 21 6 6 CD cord-285088-krim73zt 11 22 copies copy NNS cord-285088-krim73zt 11 23 per per IN cord-285088-krim73zt 11 24 μl−1 μl−1 NN cord-285088-krim73zt 11 25 of of IN cord-285088-krim73zt 11 26 pUC57 pUC57 NNP cord-285088-krim73zt 11 27 containing contain VBG cord-285088-krim73zt 11 28 N n CD cord-285088-krim73zt 11 29 gene gene NN cord-285088-krim73zt 11 30 sequence sequence NN cord-285088-krim73zt 11 31 . . . cord-285088-krim73zt 12 1 Conclusion conclusion NN cord-285088-krim73zt 13 1 This this DT cord-285088-krim73zt 13 2 study study NN cord-285088-krim73zt 13 3 provides provide VBZ cord-285088-krim73zt 13 4 rapid rapid JJ cord-285088-krim73zt 13 5 , , , cord-285088-krim73zt 13 6 reliable reliable JJ cord-285088-krim73zt 13 7 and and CC cord-285088-krim73zt 13 8 sensitive sensitive JJ cord-285088-krim73zt 13 9 tools tool NNS cord-285088-krim73zt 13 10 for for IN cord-285088-krim73zt 13 11 facilitating facilitate VBG cord-285088-krim73zt 13 12 preliminary preliminary JJ cord-285088-krim73zt 13 13 and and CC cord-285088-krim73zt 13 14 cost cost NN cord-285088-krim73zt 13 15 - - HYPH cord-285088-krim73zt 13 16 effective effective JJ cord-285088-krim73zt 13 17 prevention prevention NN cord-285088-krim73zt 13 18 and and CC cord-285088-krim73zt 13 19 control control NN cord-285088-krim73zt 13 20 of of IN cord-285088-krim73zt 13 21 COVID-19 COVID-19 NNP cord-285088-krim73zt 13 22 . . . cord-285088-krim73zt 14 1 The the DT cord-285088-krim73zt 14 2 outbreak outbreak NN cord-285088-krim73zt 14 3 of of IN cord-285088-krim73zt 14 4 COVID-19 COVID-19 NNP cord-285088-krim73zt 14 5 , , , cord-285088-krim73zt 14 6 firstly firstly RB cord-285088-krim73zt 14 7 reported report VBD cord-285088-krim73zt 14 8 from from IN cord-285088-krim73zt 14 9 Chinese chinese JJ cord-285088-krim73zt 14 10 Wuhan Wuhan NNP cord-285088-krim73zt 14 11 in in IN cord-285088-krim73zt 14 12 December December NNP cord-285088-krim73zt 14 13 2019 2019 CD cord-285088-krim73zt 14 14 , , , cord-285088-krim73zt 14 15 has have VBZ cord-285088-krim73zt 14 16 spread spread VBN cord-285088-krim73zt 14 17 to to IN cord-285088-krim73zt 14 18 many many JJ cord-285088-krim73zt 14 19 other other JJ cord-285088-krim73zt 14 20 countries country NNS cord-285088-krim73zt 14 21 in in IN cord-285088-krim73zt 14 22 the the DT cord-285088-krim73zt 14 23 last last JJ cord-285088-krim73zt 14 24 few few JJ cord-285088-krim73zt 14 25 weeks week NNS cord-285088-krim73zt 14 26 1 1 CD cord-285088-krim73zt 14 27 , , , cord-285088-krim73zt 14 28 and and CC cord-285088-krim73zt 14 29 has have VBZ cord-285088-krim73zt 14 30 caused cause VBN cord-285088-krim73zt 14 31 almost almost RB cord-285088-krim73zt 14 32 80 80 CD cord-285088-krim73zt 14 33 000 000 CD cord-285088-krim73zt 14 34 infections infection NNS cord-285088-krim73zt 14 35 and and CC cord-285088-krim73zt 14 36 more more JJR cord-285088-krim73zt 14 37 than than IN cord-285088-krim73zt 14 38 2000 2000 CD cord-285088-krim73zt 14 39 people people NNS cord-285088-krim73zt 14 40 died die VBD cord-285088-krim73zt 14 41 . . . cord-285088-krim73zt 15 1 The the DT cord-285088-krim73zt 15 2 virus virus NN cord-285088-krim73zt 15 3 spreads spread VBZ cord-285088-krim73zt 15 4 so so RB cord-285088-krim73zt 15 5 quickly quickly RB cord-285088-krim73zt 15 6 that that IN cord-285088-krim73zt 15 7 it -PRON- PRP cord-285088-krim73zt 15 8 has have VBZ cord-285088-krim73zt 15 9 attracted attract VBN cord-285088-krim73zt 15 10 the the DT cord-285088-krim73zt 15 11 globe globe NN cord-285088-krim73zt 15 12 attention attention NN cord-285088-krim73zt 15 13 , , , cord-285088-krim73zt 15 14 and and CC cord-285088-krim73zt 15 15 rapid rapid JJ cord-285088-krim73zt 15 16 diagnosis diagnosis NN cord-285088-krim73zt 15 17 is be VBZ cord-285088-krim73zt 15 18 one one CD cord-285088-krim73zt 15 19 of of IN cord-285088-krim73zt 15 20 the the DT cord-285088-krim73zt 15 21 effective effective JJ cord-285088-krim73zt 15 22 ways way NNS cord-285088-krim73zt 15 23 to to TO cord-285088-krim73zt 15 24 prevent prevent VB cord-285088-krim73zt 15 25 and and CC cord-285088-krim73zt 15 26 control control VB cord-285088-krim73zt 15 27 the the DT cord-285088-krim73zt 15 28 diseases disease NNS cord-285088-krim73zt 15 29 . . . cord-285088-krim73zt 16 1 Chest Chest NNP cord-285088-krim73zt 16 2 CT CT NNP cord-285088-krim73zt 16 3 and and CC cord-285088-krim73zt 16 4 RT RT NNP cord-285088-krim73zt 16 5 - - HYPH cord-285088-krim73zt 16 6 PCR PCR NNP cord-285088-krim73zt 16 7 has have VBZ cord-285088-krim73zt 16 8 been be VBN cord-285088-krim73zt 16 9 used use VBN cord-285088-krim73zt 16 10 for for IN cord-285088-krim73zt 16 11 the the DT cord-285088-krim73zt 16 12 clinical clinical JJ cord-285088-krim73zt 16 13 diagnosis diagnosis NN cord-285088-krim73zt 16 14 of of IN cord-285088-krim73zt 16 15 the the DT cord-285088-krim73zt 16 16 coronavirus coronavirus NN cord-285088-krim73zt 16 17 pneumonia pneumonia NN cord-285088-krim73zt 16 18 2 2 CD cord-285088-krim73zt 16 19 , , , cord-285088-krim73zt 16 20 3 3 CD cord-285088-krim73zt 16 21 , , , cord-285088-krim73zt 16 22 however however RB cord-285088-krim73zt 16 23 , , , cord-285088-krim73zt 16 24 chest chest NN cord-285088-krim73zt 16 25 CT CT NNP cord-285088-krim73zt 16 26 needs need VBZ cord-285088-krim73zt 16 27 CT CT NNP cord-285088-krim73zt 16 28 equipment equipment NN cord-285088-krim73zt 16 29 and and CC cord-285088-krim73zt 16 30 put put VB cord-285088-krim73zt 16 31 the the DT cord-285088-krim73zt 16 32 professional professional JJ cord-285088-krim73zt 16 33 operator operator NN cord-285088-krim73zt 16 34 at at IN cord-285088-krim73zt 16 35 risk risk NN cord-285088-krim73zt 16 36 of of IN cord-285088-krim73zt 16 37 infection infection NN cord-285088-krim73zt 16 38 , , , cord-285088-krim73zt 16 39 and and CC cord-285088-krim73zt 16 40 RT RT NNP cord-285088-krim73zt 16 41 - - HYPH cord-285088-krim73zt 16 42 PCR PCR NNP cord-285088-krim73zt 16 43 has have VBZ cord-285088-krim73zt 16 44 high high JJ cord-285088-krim73zt 16 45 professional professional JJ cord-285088-krim73zt 16 46 and and CC cord-285088-krim73zt 16 47 technical technical JJ cord-285088-krim73zt 16 48 requirements requirement NNS cord-285088-krim73zt 16 49 for for IN cord-285088-krim73zt 16 50 operators operator NNS cord-285088-krim73zt 16 51 . . . cord-285088-krim73zt 17 1 Loop loop NN cord-285088-krim73zt 17 2 - - HYPH cord-285088-krim73zt 17 3 mediated mediate VBN cord-285088-krim73zt 17 4 isothermal isothermal JJ cord-285088-krim73zt 17 5 amplification amplification NN cord-285088-krim73zt 17 6 ( ( -LRB- cord-285088-krim73zt 17 7 LAMP lamp NN cord-285088-krim73zt 17 8 ) ) -RRB- cord-285088-krim73zt 17 9 can can MD cord-285088-krim73zt 17 10 amplify amplify VB cord-285088-krim73zt 17 11 nucleic nucleic JJ cord-285088-krim73zt 17 12 acids acid NNS cord-285088-krim73zt 17 13 under under IN cord-285088-krim73zt 17 14 isothermal isothermal JJ cord-285088-krim73zt 17 15 conditions condition NNS cord-285088-krim73zt 17 16 4 4 CD cord-285088-krim73zt 17 17 , , , cord-285088-krim73zt 17 18 and and CC cord-285088-krim73zt 17 19 it -PRON- PRP cord-285088-krim73zt 17 20 had have VBD cord-285088-krim73zt 17 21 the the DT cord-285088-krim73zt 17 22 advantages advantage NNS cord-285088-krim73zt 17 23 over over IN cord-285088-krim73zt 17 24 real real JJ cord-285088-krim73zt 17 25 - - HYPH cord-285088-krim73zt 17 26 time time NN cord-285088-krim73zt 17 27 PCR pcr NN cord-285088-krim73zt 17 28 assays assay NNS cord-285088-krim73zt 17 29 in in IN cord-285088-krim73zt 17 30 specificity specificity NN cord-285088-krim73zt 17 31 sensitivity sensitivity NN cord-285088-krim73zt 17 32 , , , cord-285088-krim73zt 17 33 cost cost NN cord-285088-krim73zt 17 34 effectiveness effectiveness NN cord-285088-krim73zt 17 35 and and CC cord-285088-krim73zt 17 36 rapidity rapidity NN cord-285088-krim73zt 17 37 5 5 CD cord-285088-krim73zt 17 38 , , , cord-285088-krim73zt 17 39 6 6 CD cord-285088-krim73zt 17 40 , , , cord-285088-krim73zt 17 41 7 7 CD cord-285088-krim73zt 17 42 . . . cord-285088-krim73zt 18 1 The the DT cord-285088-krim73zt 18 2 objective objective NN cord-285088-krim73zt 18 3 of of IN cord-285088-krim73zt 18 4 this this DT cord-285088-krim73zt 18 5 study study NN cord-285088-krim73zt 18 6 was be VBD cord-285088-krim73zt 18 7 to to TO cord-285088-krim73zt 18 8 develop develop VB cord-285088-krim73zt 18 9 the the DT cord-285088-krim73zt 18 10 one one CD cord-285088-krim73zt 18 11 - - HYPH cord-285088-krim73zt 18 12 pot pot NN cord-285088-krim73zt 18 13 real real JJ cord-285088-krim73zt 18 14 - - HYPH cord-285088-krim73zt 18 15 time time NN cord-285088-krim73zt 18 16 RT RT NNP cord-285088-krim73zt 18 17 - - HYPH cord-285088-krim73zt 18 18 LAMP LAMP NNP cord-285088-krim73zt 18 19 assay assay NN cord-285088-krim73zt 18 20 and and CC cord-285088-krim73zt 18 21 the the DT cord-285088-krim73zt 18 22 one one CD cord-285088-krim73zt 18 23 - - HYPH cord-285088-krim73zt 18 24 pot pot NN cord-285088-krim73zt 18 25 visual visual JJ cord-285088-krim73zt 18 26 RT rt NN cord-285088-krim73zt 18 27 - - HYPH cord-285088-krim73zt 18 28 LAMP LAMP NNP cord-285088-krim73zt 18 29 assay assay NN cord-285088-krim73zt 18 30 for for IN cord-285088-krim73zt 18 31 diagnosis diagnosis NN cord-285088-krim73zt 18 32 of of IN cord-285088-krim73zt 18 33 the the DT cord-285088-krim73zt 18 34 pneumonia pneumonia NN cord-285088-krim73zt 18 35 caused cause VBN cord-285088-krim73zt 18 36 by by IN cord-285088-krim73zt 18 37 COVID-19 COVID-19 NNP cord-285088-krim73zt 18 38 , , , cord-285088-krim73zt 18 39 which which WDT cord-285088-krim73zt 18 40 would would MD cord-285088-krim73zt 18 41 be be VB cord-285088-krim73zt 18 42 suitable suitable JJ cord-285088-krim73zt 18 43 for for IN cord-285088-krim73zt 18 44 use use NN cord-285088-krim73zt 18 45 at at IN cord-285088-krim73zt 18 46 less less RBR cord-285088-krim73zt 18 47 developed develop VBN cord-285088-krim73zt 18 48 areas area NNS cord-285088-krim73zt 18 49 . . . cord-285088-krim73zt 19 1 The the DT cord-285088-krim73zt 19 2 RT RT NNP cord-285088-krim73zt 19 3 - - HYPH cord-285088-krim73zt 19 4 LAMP LAMP NNP cord-285088-krim73zt 19 5 primers primer NNS cord-285088-krim73zt 19 6 targeting target VBG cord-285088-krim73zt 19 7 the the DT cord-285088-krim73zt 19 8 N n CD cord-285088-krim73zt 19 9 gene gene NN cord-285088-krim73zt 19 10 of of IN cord-285088-krim73zt 19 11 COVID-19 COVID-19 NNP cord-285088-krim73zt 19 12 ( ( -LRB- cord-285088-krim73zt 19 13 GenBank GenBank NNP cord-285088-krim73zt 19 14 accession accession NN cord-285088-krim73zt 20 1 No no UH cord-285088-krim73zt 20 2 . . . cord-285088-krim73zt 20 3 MN997409.1 MN997409.1 NNP cord-285088-krim73zt 20 4 ) ) -RRB- cord-285088-krim73zt 20 5 were be VBD cord-285088-krim73zt 20 6 designed design VBN cord-285088-krim73zt 20 7 using use VBG cord-285088-krim73zt 20 8 PrimerExplorer PrimerExplorer NNP cord-285088-krim73zt 20 9 V5 V5 NNP cord-285088-krim73zt 20 10 ( ( -LRB- cord-285088-krim73zt 20 11 http://primerexplorer.jp/e/ http://primerexplorer.jp/e/ NNP cord-285088-krim73zt 20 12 ) ) -RRB- cord-285088-krim73zt 20 13 and and CC cord-285088-krim73zt 20 14 Oligo Oligo NNP cord-285088-krim73zt 20 15 7 7 CD cord-285088-krim73zt 20 16 ( ( -LRB- cord-285088-krim73zt 20 17 Molecular Molecular NNP cord-285088-krim73zt 20 18 Biology Biology NNP cord-285088-krim73zt 20 19 Insights Insights NNPS cord-285088-krim73zt 20 20 , , , cord-285088-krim73zt 21 1 Inc. Inc. NNP cord-285088-krim73zt 21 2 Colorado Colorado NNP cord-285088-krim73zt 21 3 Springs Springs NNP cord-285088-krim73zt 21 4 , , , cord-285088-krim73zt 21 5 CO CO NNP cord-285088-krim73zt 21 6 , , , cord-285088-krim73zt 21 7 USA USA NNP cord-285088-krim73zt 21 8 ) ) -RRB- cord-285088-krim73zt 21 9 software software NN cord-285088-krim73zt 21 10 packages package NNS cord-285088-krim73zt 21 11 . . . cord-285088-krim73zt 22 1 The the DT cord-285088-krim73zt 22 2 primer primer NN cord-285088-krim73zt 22 3 sequences sequence NNS cord-285088-krim73zt 22 4 are be VBP cord-285088-krim73zt 22 5 GCC GCC NNP cord-285088-krim73zt 22 6 AAA AAA NNP cord-285088-krim73zt 22 7 AGG agg NN cord-285088-krim73zt 22 8 CTT CTT NNP cord-285088-krim73zt 22 9 CTA CTA NNP cord-285088-krim73zt 22 10 CGC CGC NNP cord-285088-krim73zt 22 11 A A NNP cord-285088-krim73zt 22 12 ( ( -LRB- cord-285088-krim73zt 22 13 F3 F3 NNP cord-285088-krim73zt 22 14 ) ) -RRB- cord-285088-krim73zt 22 15 , , . cord-285088-krim73zt 23 1 TTT TTT NNP cord-285088-krim73zt 23 2 GGC GGC NNP cord-285088-krim73zt 23 3 CTT CTT NNP cord-285088-krim73zt 23 4 GTT GTT NNP cord-285088-krim73zt 23 5 GTT GTT NNP cord-285088-krim73zt 23 6 GTT GTT NNP cord-285088-krim73zt 23 7 GG GG NNP cord-285088-krim73zt 23 8 ( ( -LRB- cord-285088-krim73zt 23 9 B3 b3 NN cord-285088-krim73zt 23 10 ) ) -RRB- cord-285088-krim73zt 24 1 , , , cord-285088-krim73zt 24 2 TCC TCC NNP cord-285088-krim73zt 24 3 CCT CCT NNP cord-285088-krim73zt 24 4 ACT ACT NNP cord-285088-krim73zt 25 1 GCT GCT NNP cord-285088-krim73zt 25 2 GCC GCC NNP cord-285088-krim73zt 25 3 _SP cord-285088-krim73zt 26 1 TGG TGG NNP cord-285088-krim73zt 26 2 AGT AGT NNP cord-285088-krim73zt 26 3 TTT TTT NNP cord-285088-krim73zt 26 4 CGG CGG NNP cord-285088-krim73zt 26 5 CAG CAG NNP cord-285088-krim73zt 27 1 TCA TCA NNP cord-285088-krim73zt 27 2 AGC AGC NNP cord-285088-krim73zt 27 3 CTC CTC NNP cord-285088-krim73zt 27 4 TTC TTC NNP cord-285088-krim73zt 27 5 ( ( -LRB- cord-285088-krim73zt 27 6 FIP FIP NNP cord-285088-krim73zt 27 7 ) ) -RRB- cord-285088-krim73zt 27 8 , , , cord-285088-krim73zt 27 9 TCC TCC NNP cord-285088-krim73zt 27 10 TGC TGC NNP cord-285088-krim73zt 27 11 TAG tag NN cord-285088-krim73zt 27 12 AAT AAT NNP cord-285088-krim73zt 27 13 GGC GGC NNP cord-285088-krim73zt 28 1 TGG TGG NNP cord-285088-krim73zt 28 2 CAA CAA NNP cord-285088-krim73zt 28 3 TTT TTT NNP cord-285088-krim73zt 28 4 _SP cord-285088-krim73zt 28 5 TTT TTT NNP cord-285088-krim73zt 29 1 TTG TTG NNP cord-285088-krim73zt 29 2 CTC CTC NNP cord-285088-krim73zt 29 3 TCA TCA NNP cord-285088-krim73zt 29 4 AGC AGC NNP cord-285088-krim73zt 30 1 TGG TGG NNP cord-285088-krim73zt 30 2 TTC TTC NNP cord-285088-krim73zt 30 3 A A NNP cord-285088-krim73zt 30 4 ( ( -LRB- cord-285088-krim73zt 30 5 BIP BIP NNP cord-285088-krim73zt 30 6 ) ) -RRB- cord-285088-krim73zt 31 1 , , , cord-285088-krim73zt 31 2 CGA CGA NNP cord-285088-krim73zt 31 3 CTA CTA NNP cord-285088-krim73zt 31 4 CGT CGT NNP cord-285088-krim73zt 31 5 GAT GAT NNP cord-285088-krim73zt 31 6 GAG GAG NNP cord-285088-krim73zt 31 7 GAA GAA NNP cord-285088-krim73zt 31 8 CGA CGA NNP cord-285088-krim73zt 31 9 ( ( -LRB- cord-285088-krim73zt 31 10 LF LF NNP cord-285088-krim73zt 31 11 ) ) -RRB- cord-285088-krim73zt 31 12 and and CC cord-285088-krim73zt 31 13 GCG GCG NNP cord-285088-krim73zt 31 14 GTG GTG NNP cord-285088-krim73zt 31 15 ATG ATG NNP cord-285088-krim73zt 31 16 CTG CTG NNP cord-285088-krim73zt 31 17 CTC CTC NNP cord-285088-krim73zt 31 18 T T NNP cord-285088-krim73zt 31 19 ( ( -LRB- cord-285088-krim73zt 31 20 LB LB NNP cord-285088-krim73zt 31 21 ) ) -RRB- cord-285088-krim73zt 31 22 , , , cord-285088-krim73zt 31 23 Table table NN cord-285088-krim73zt 31 24 1 1 CD cord-285088-krim73zt 31 25 , , , cord-285088-krim73zt 31 26 and and CC cord-285088-krim73zt 31 27 the the DT cord-285088-krim73zt 31 28 length length NN cord-285088-krim73zt 31 29 of of IN cord-285088-krim73zt 31 30 the the DT cord-285088-krim73zt 31 31 targeted target VBN cord-285088-krim73zt 31 32 sequence sequence NN cord-285088-krim73zt 31 33 was be VBD cord-285088-krim73zt 31 34 233 233 CD cord-285088-krim73zt 31 35 bp bp NNP cord-285088-krim73zt 31 36 . . . cord-285088-krim73zt 32 1 The the DT cord-285088-krim73zt 32 2 N n CD cord-285088-krim73zt 32 3 gene gene NN cord-285088-krim73zt 32 4 ( ( -LRB- cord-285088-krim73zt 32 5 GenBank GenBank NNP cord-285088-krim73zt 32 6 accession accession NN cord-285088-krim73zt 33 1 No no UH cord-285088-krim73zt 33 2 . . . cord-285088-krim73zt 33 3 MN997409.1 MN997409.1 NNP cord-285088-krim73zt 33 4 ) ) -RRB- cord-285088-krim73zt 34 1 of of IN cord-285088-krim73zt 34 2 COVID COVID NNP cord-285088-krim73zt 34 3 was be VBD cord-285088-krim73zt 34 4 chemically chemically RB cord-285088-krim73zt 34 5 synthesized synthesize VBN cord-285088-krim73zt 34 6 and and CC cord-285088-krim73zt 34 7 cloned clone VBN cord-285088-krim73zt 34 8 into into IN cord-285088-krim73zt 34 9 pUC57 puc57 NN cord-285088-krim73zt 34 10 plasmid plasmid NNP cord-285088-krim73zt 34 11 ( ( -LRB- cord-285088-krim73zt 34 12 herein herein RB cord-285088-krim73zt 34 13 referred refer VBN cord-285088-krim73zt 34 14 to to IN cord-285088-krim73zt 34 15 as as IN cord-285088-krim73zt 34 16 pUC57-N puc57-n CD cord-285088-krim73zt 34 17 DNA DNA NNP cord-285088-krim73zt 34 18 ) ) -RRB- cord-285088-krim73zt 34 19 by by IN cord-285088-krim73zt 34 20 General General NNP cord-285088-krim73zt 34 21 Biosystems Biosystems NNP cord-285088-krim73zt 34 22 ( ( -LRB- cord-285088-krim73zt 34 23 Anhui Anhui NNP cord-285088-krim73zt 34 24 ) ) -RRB- cord-285088-krim73zt 34 25 Co. Co. NNP cord-285088-krim73zt 34 26 , , , cord-285088-krim73zt 34 27 Ltd Ltd NNP cord-285088-krim73zt 34 28 , , , cord-285088-krim73zt 34 29 the the DT cord-285088-krim73zt 34 30 pUC57-N pUC57-N NNP cord-285088-krim73zt 34 31 DNA DNA NNP cord-285088-krim73zt 34 32 was be VBD cord-285088-krim73zt 34 33 used use VBN cord-285088-krim73zt 34 34 as as IN cord-285088-krim73zt 34 35 the the DT cord-285088-krim73zt 34 36 template template NN cord-285088-krim73zt 34 37 for for IN cord-285088-krim73zt 34 38 optimization optimization NN cord-285088-krim73zt 34 39 of of IN cord-285088-krim73zt 34 40 the the DT cord-285088-krim73zt 34 41 RT RT NNP cord-285088-krim73zt 34 42 - - HYPH cord-285088-krim73zt 34 43 LAMP LAMP NNP cord-285088-krim73zt 34 44 system system NN cord-285088-krim73zt 34 45 , , , cord-285088-krim73zt 34 46 as as RB cord-285088-krim73zt 34 47 well well RB cord-285088-krim73zt 34 48 as as IN cord-285088-krim73zt 34 49 for for IN cord-285088-krim73zt 34 50 determination determination NN cord-285088-krim73zt 34 51 of of IN cord-285088-krim73zt 34 52 sensitivity sensitivity NN cord-285088-krim73zt 34 53 . . . cord-285088-krim73zt 35 1 The the DT cord-285088-krim73zt 35 2 real real JJ cord-285088-krim73zt 35 3 - - HYPH cord-285088-krim73zt 35 4 time time NN cord-285088-krim73zt 35 5 RT RT NNP cord-285088-krim73zt 35 6 - - HYPH cord-285088-krim73zt 35 7 LAMP LAMP NNP cord-285088-krim73zt 35 8 assay assay NN cord-285088-krim73zt 35 9 with with IN cord-285088-krim73zt 35 10 above above RB cord-285088-krim73zt 35 11 designed design VBN cord-285088-krim73zt 35 12 RT RT NNP cord-285088-krim73zt 35 13 - - HYPH cord-285088-krim73zt 35 14 LAMP LAMP NNP cord-285088-krim73zt 35 15 primers primer NNS cord-285088-krim73zt 35 16 was be VBD cord-285088-krim73zt 35 17 performed perform VBN cord-285088-krim73zt 35 18 in in IN cord-285088-krim73zt 35 19 a a DT cord-285088-krim73zt 35 20 50-μL 50-μl CD cord-285088-krim73zt 35 21 reaction reaction NN cord-285088-krim73zt 35 22 mixture mixture NN cord-285088-krim73zt 35 23 containing contain VBG cord-285088-krim73zt 35 24 0.8 0.8 CD cord-285088-krim73zt 35 25 mM mm CD cord-285088-krim73zt 35 26 each each DT cord-285088-krim73zt 35 27 of of IN cord-285088-krim73zt 35 28 forward forward JJ cord-285088-krim73zt 35 29 inner inner JJ cord-285088-krim73zt 35 30 primer primer NN cord-285088-krim73zt 35 31 ( ( -LRB- cord-285088-krim73zt 35 32 FIP FIP NNP cord-285088-krim73zt 35 33 ) ) -RRB- cord-285088-krim73zt 35 34 and and CC cord-285088-krim73zt 35 35 backward backward JJ cord-285088-krim73zt 35 36 inner inner JJ cord-285088-krim73zt 35 37 primer primer NN cord-285088-krim73zt 35 38 ( ( -LRB- cord-285088-krim73zt 35 39 BIP BIP NNP cord-285088-krim73zt 35 40 ) ) -RRB- cord-285088-krim73zt 35 41 , , , cord-285088-krim73zt 35 42 0.2 0.2 CD cord-285088-krim73zt 35 43 mM mm CD cord-285088-krim73zt 35 44 each each DT cord-285088-krim73zt 35 45 of of IN cord-285088-krim73zt 35 46 forward forward RB cord-285088-krim73zt 35 47 outer outer JJ cord-285088-krim73zt 35 48 primer primer NN cord-285088-krim73zt 35 49 ( ( -LRB- cord-285088-krim73zt 35 50 F3 F3 NNP cord-285088-krim73zt 35 51 ) ) -RRB- cord-285088-krim73zt 35 52 and and CC cord-285088-krim73zt 35 53 backward backward JJ cord-285088-krim73zt 35 54 outer outer JJ cord-285088-krim73zt 35 55 primer primer NN cord-285088-krim73zt 35 56 ( ( -LRB- cord-285088-krim73zt 35 57 B3 B3 NNP cord-285088-krim73zt 35 58 ) ) -RRB- cord-285088-krim73zt 35 59 , , , cord-285088-krim73zt 35 60 0.4 0.4 CD cord-285088-krim73zt 35 61 mM mm CD cord-285088-krim73zt 35 62 of of IN cord-285088-krim73zt 35 63 forward forward JJ cord-285088-krim73zt 35 64 loop loop NN cord-285088-krim73zt 35 65 primer primer NN cord-285088-krim73zt 35 66 ( ( -LRB- cord-285088-krim73zt 35 67 LF LF NNP cord-285088-krim73zt 35 68 ) ) -RRB- cord-285088-krim73zt 35 69 and and CC cord-285088-krim73zt 35 70 backward backward JJ cord-285088-krim73zt 35 71 loop loop NN cord-285088-krim73zt 35 72 primer primer NN cord-285088-krim73zt 35 73 ( ( -LRB- cord-285088-krim73zt 35 74 LB LB NNP cord-285088-krim73zt 35 75 ) ) -RRB- cord-285088-krim73zt 35 76 , , , cord-285088-krim73zt 35 77 1.2 1.2 CD cord-285088-krim73zt 35 78 mM mm CD cord-285088-krim73zt 35 79 dNTPs dntps CD cord-285088-krim73zt 35 80 , , . cord-285088-krim73zt 36 1 1× 1× CD cord-285088-krim73zt 36 2 Bst Bst NNP cord-285088-krim73zt 36 3 DNA dna NN cord-285088-krim73zt 36 4 Polymerase polymerase NN cord-285088-krim73zt 36 5 Buffer Buffer NNP cord-285088-krim73zt 36 6 ( ( -LRB- cord-285088-krim73zt 36 7 Zhengzhou Zhengzhou NNP cord-285088-krim73zt 36 8 Shenxiang Shenxiang NNP cord-285088-krim73zt 36 9 Industrial Industrial NNP cord-285088-krim73zt 36 10 Co. Co. NNP cord-285088-krim73zt 36 11 , , , cord-285088-krim73zt 36 12 Ltd Ltd NNP cord-285088-krim73zt 36 13 , , , cord-285088-krim73zt 36 14 China China NNP cord-285088-krim73zt 36 15 ) ) -RRB- cord-285088-krim73zt 36 16 , , , cord-285088-krim73zt 36 17 1 1 CD cord-285088-krim73zt 36 18 × × NNP cord-285088-krim73zt 36 19 EvaGreen EvaGreen NNP cord-285088-krim73zt 36 20 , , , cord-285088-krim73zt 36 21 1 1 CD cord-285088-krim73zt 36 22 × × NNP cord-285088-krim73zt 36 23 Rox Rox NNP cord-285088-krim73zt 36 24 , , , cord-285088-krim73zt 36 25 1 1 CD cord-285088-krim73zt 36 26 pg pg NN cord-285088-krim73zt 36 27 pUC57-N puc57-n CC cord-285088-krim73zt 36 28 DNA DNA NNP cord-285088-krim73zt 36 29 , , , cord-285088-krim73zt 36 30 and and CC cord-285088-krim73zt 36 31 25 25 CD cord-285088-krim73zt 36 32 U U NNP cord-285088-krim73zt 36 33 Bst Bst NNP cord-285088-krim73zt 36 34 DNA DNA NNP cord-285088-krim73zt 36 35 / / SYM cord-285088-krim73zt 36 36 RNA RNA NNP cord-285088-krim73zt 36 37 Polymerase Polymerase NNP cord-285088-krim73zt 36 38 3.0 3.0 CD cord-285088-krim73zt 37 1 ( ( -LRB- cord-285088-krim73zt 37 2 New New NNP cord-285088-krim73zt 37 3 England England NNP cord-285088-krim73zt 37 4 Biolabs Biolabs NNP cord-285088-krim73zt 37 5 , , , cord-285088-krim73zt 37 6 Inc. Inc. NNP cord-285088-krim73zt 37 7 , , , cord-285088-krim73zt 37 8 MA MA NNP cord-285088-krim73zt 37 9 , , , cord-285088-krim73zt 37 10 USA USA NNP cord-285088-krim73zt 37 11 ) ) -RRB- cord-285088-krim73zt 37 12 8 8 CD cord-285088-krim73zt 37 13 , , , cord-285088-krim73zt 37 14 9 9 CD cord-285088-krim73zt 37 15 . . . cord-285088-krim73zt 38 1 The the DT cord-285088-krim73zt 38 2 reaction reaction NN cord-285088-krim73zt 38 3 mixtures mixture NNS cord-285088-krim73zt 38 4 were be VBD cord-285088-krim73zt 38 5 heated heat VBN cord-285088-krim73zt 38 6 at at IN cord-285088-krim73zt 38 7 55 55 CD cord-285088-krim73zt 38 8 ° ° NN cord-285088-krim73zt 38 9 C c NN cord-285088-krim73zt 38 10 , , , cord-285088-krim73zt 38 11 57 57 CD cord-285088-krim73zt 38 12 ° ° NN cord-285088-krim73zt 38 13 C c NN cord-285088-krim73zt 38 14 , , , cord-285088-krim73zt 38 15 59 59 CD cord-285088-krim73zt 38 16 ° ° NN cord-285088-krim73zt 38 17 C c NN cord-285088-krim73zt 38 18 and and CC cord-285088-krim73zt 38 19 61 61 CD cord-285088-krim73zt 38 20 ° ° NN cord-285088-krim73zt 38 21 C c NN cord-285088-krim73zt 38 22 for for IN cord-285088-krim73zt 38 23 50 50 CD cord-285088-krim73zt 38 24 min min NN cord-285088-krim73zt 38 25 ( ( -LRB- cord-285088-krim73zt 38 26 30 30 CD cord-285088-krim73zt 38 27 s s NN cord-285088-krim73zt 38 28 per per IN cord-285088-krim73zt 38 29 cycle cycle NN cord-285088-krim73zt 38 30 ) ) -RRB- cord-285088-krim73zt 38 31 , , , cord-285088-krim73zt 38 32 individually individually RB cord-285088-krim73zt 38 33 . . . cord-285088-krim73zt 39 1 The the DT cord-285088-krim73zt 39 2 amplification amplification NN cord-285088-krim73zt 39 3 plot plot NN cord-285088-krim73zt 39 4 and and CC cord-285088-krim73zt 39 5 melt melt VB cord-285088-krim73zt 39 6 curve curve NN cord-285088-krim73zt 39 7 were be VBD cord-285088-krim73zt 39 8 obtained obtain VBN cord-285088-krim73zt 39 9 using use VBG cord-285088-krim73zt 39 10 a a DT cord-285088-krim73zt 39 11 StepOne StepOne NNP cord-285088-krim73zt 39 12 TM TM NNP cord-285088-krim73zt 39 13 System System NNP cord-285088-krim73zt 39 14 ( ( -LRB- cord-285088-krim73zt 39 15 Applied Applied NNP cord-285088-krim73zt 39 16 Biosystems Biosystems NNP cord-285088-krim73zt 39 17 , , , cord-285088-krim73zt 39 18 Foster Foster NNP cord-285088-krim73zt 39 19 City City NNP cord-285088-krim73zt 39 20 , , , cord-285088-krim73zt 39 21 CA CA NNP cord-285088-krim73zt 39 22 , , , cord-285088-krim73zt 39 23 USA USA NNP cord-285088-krim73zt 39 24 ) ) -RRB- cord-285088-krim73zt 39 25 . . . cord-285088-krim73zt 40 1 Because because IN cord-285088-krim73zt 40 2 the the DT cord-285088-krim73zt 40 3 RNA RNA NNP cord-285088-krim73zt 40 4 extraction extraction NN cord-285088-krim73zt 40 5 step step NN cord-285088-krim73zt 40 6 was be VBD cord-285088-krim73zt 40 7 omitted omit VBN cord-285088-krim73zt 40 8 , , , cord-285088-krim73zt 40 9 the the DT cord-285088-krim73zt 40 10 effect effect NN cord-285088-krim73zt 40 11 of of IN cord-285088-krim73zt 40 12 inhibitors inhibitor NNS cord-285088-krim73zt 40 13 in in IN cord-285088-krim73zt 40 14 blood blood NN cord-285088-krim73zt 40 15 samples sample NNS cord-285088-krim73zt 40 16 on on IN cord-285088-krim73zt 40 17 the the DT cord-285088-krim73zt 40 18 amplification amplification NN cord-285088-krim73zt 40 19 efficiency efficiency NN cord-285088-krim73zt 40 20 of of IN cord-285088-krim73zt 40 21 the the DT cord-285088-krim73zt 40 22 RT RT NNP cord-285088-krim73zt 40 23 - - HYPH cord-285088-krim73zt 40 24 LAMP LAMP NNP cord-285088-krim73zt 40 25 assays assay NNS cord-285088-krim73zt 40 26 was be VBD cord-285088-krim73zt 40 27 to to TO cord-285088-krim73zt 40 28 be be VB cord-285088-krim73zt 40 29 determined determine VBN cord-285088-krim73zt 40 30 . . . cord-285088-krim73zt 41 1 10 10 CD cord-285088-krim73zt 41 2 µL µL NNP cord-285088-krim73zt 41 3 , , , cord-285088-krim73zt 41 4 7.5µL 7.5µl CD cord-285088-krim73zt 41 5 , , , cord-285088-krim73zt 41 6 5 5 CD cord-285088-krim73zt 41 7 µL µl CD cord-285088-krim73zt 41 8 and and CC cord-285088-krim73zt 41 9 2.5 2.5 CD cord-285088-krim73zt 41 10 µL µl XX cord-285088-krim73zt 41 11 pork pork NN cord-285088-krim73zt 41 12 blood blood NN cord-285088-krim73zt 41 13 samples sample NNS cord-285088-krim73zt 41 14 ( ( -LRB- cord-285088-krim73zt 41 15 purchased purchase VBN cord-285088-krim73zt 41 16 from from IN cord-285088-krim73zt 41 17 Xuchang Xuchang NNP cord-285088-krim73zt 41 18 Market Market NNP cord-285088-krim73zt 41 19 , , , cord-285088-krim73zt 41 20 China China NNP cord-285088-krim73zt 41 21 ) ) -RRB- cord-285088-krim73zt 41 22 free free JJ cord-285088-krim73zt 41 23 of of IN cord-285088-krim73zt 41 24 COVID-19 COVID-19 NNP cord-285088-krim73zt 41 25 were be VBD cord-285088-krim73zt 41 26 dissolved dissolve VBN cord-285088-krim73zt 41 27 in in IN cord-285088-krim73zt 41 28 10 10 CD cord-285088-krim73zt 41 29 µL µl CD cord-285088-krim73zt 41 30 2019-nCoV 2019-ncov CD cord-285088-krim73zt 41 31 - - HYPH cord-285088-krim73zt 41 32 Fast fast JJ cord-285088-krim73zt 41 33 - - HYPH cord-285088-krim73zt 41 34 Sample sample NN cord-285088-krim73zt 41 35 Nucleic Nucleic NNS cord-285088-krim73zt 41 36 Acid acid NN cord-285088-krim73zt 41 37 Releasing release VBG cord-285088-krim73zt 41 38 Agent Agent NNP cord-285088-krim73zt 41 39 , , , cord-285088-krim73zt 41 40 respectively respectively RB cord-285088-krim73zt 41 41 , , , cord-285088-krim73zt 41 42 which which WDT cord-285088-krim73zt 41 43 were be VBD cord-285088-krim73zt 41 44 added add VBN cord-285088-krim73zt 41 45 to to IN cord-285088-krim73zt 41 46 above above IN cord-285088-krim73zt 41 47 50 50 CD cord-285088-krim73zt 41 48 µL µL NNP cord-285088-krim73zt 41 49 real real JJ cord-285088-krim73zt 41 50 - - HYPH cord-285088-krim73zt 41 51 time time NN cord-285088-krim73zt 41 52 RT rt NN cord-285088-krim73zt 41 53 - - HYPH cord-285088-krim73zt 41 54 LAMP LAMP NNP cord-285088-krim73zt 41 55 reaction reaction NN cord-285088-krim73zt 41 56 system system NN cord-285088-krim73zt 41 57 and and CC cord-285088-krim73zt 41 58 heated heat VBN cord-285088-krim73zt 41 59 at at IN cord-285088-krim73zt 41 60 the the DT cord-285088-krim73zt 41 61 optimized optimize VBN cord-285088-krim73zt 41 62 temperature temperature NN cord-285088-krim73zt 41 63 for for IN cord-285088-krim73zt 41 64 50 50 CD cord-285088-krim73zt 41 65 min min NN cord-285088-krim73zt 41 66 ( ( -LRB- cord-285088-krim73zt 41 67 30 30 CD cord-285088-krim73zt 41 68 s s NN cord-285088-krim73zt 41 69 per per IN cord-285088-krim73zt 41 70 cycle cycle NN cord-285088-krim73zt 41 71 ) ) -RRB- cord-285088-krim73zt 41 72 in in IN cord-285088-krim73zt 41 73 StepOne StepOne NNP cord-285088-krim73zt 41 74 TM TM NNP cord-285088-krim73zt 41 75 System System NNP cord-285088-krim73zt 41 76 ( ( -LRB- cord-285088-krim73zt 41 77 Applied Applied NNP cord-285088-krim73zt 41 78 Biosystems Biosystems NNP cord-285088-krim73zt 41 79 , , , cord-285088-krim73zt 41 80 Foster Foster NNP cord-285088-krim73zt 41 81 City City NNP cord-285088-krim73zt 41 82 , , , cord-285088-krim73zt 41 83 CA CA NNP cord-285088-krim73zt 41 84 , , , cord-285088-krim73zt 41 85 USA USA NNP cord-285088-krim73zt 41 86 ) ) -RRB- cord-285088-krim73zt 41 87 . . . cord-285088-krim73zt 42 1 The the DT cord-285088-krim73zt 42 2 analytic analytic JJ cord-285088-krim73zt 42 3 sensitivities sensitivity NNS cord-285088-krim73zt 42 4 of of IN cord-285088-krim73zt 42 5 the the DT cord-285088-krim73zt 42 6 newly newly RB cord-285088-krim73zt 42 7 developed develop VBN cord-285088-krim73zt 42 8 real real JJ cord-285088-krim73zt 42 9 - - HYPH cord-285088-krim73zt 42 10 time time NN cord-285088-krim73zt 42 11 RT RT NNP cord-285088-krim73zt 42 12 - - HYPH cord-285088-krim73zt 42 13 LAMP LAMP NNP cord-285088-krim73zt 42 14 assay assay NN cord-285088-krim73zt 42 15 and and CC cord-285088-krim73zt 42 16 visual visual JJ cord-285088-krim73zt 42 17 RT RT NNP cord-285088-krim73zt 42 18 - - HYPH cord-285088-krim73zt 42 19 LAMP LAMP NNP cord-285088-krim73zt 42 20 assay assay NN cord-285088-krim73zt 42 21 were be VBD cord-285088-krim73zt 42 22 determined determine VBN cord-285088-krim73zt 42 23 with with IN cord-285088-krim73zt 42 24 pUC57-N puc57-n CC cord-285088-krim73zt 42 25 DNA dna NN cord-285088-krim73zt 42 26 ranging range VBG cord-285088-krim73zt 42 27 from from IN cord-285088-krim73zt 42 28 2 2 CD cord-285088-krim73zt 42 29 - - HYPH cord-285088-krim73zt 42 30 0.02 0.02 CD cord-285088-krim73zt 42 31 fg fg NN cord-285088-krim73zt 42 32 , , , cord-285088-krim73zt 42 33 and and CC cord-285088-krim73zt 42 34 the the DT cord-285088-krim73zt 42 35 reaction reaction NN cord-285088-krim73zt 42 36 mixtures mixture NNS cord-285088-krim73zt 42 37 were be VBD cord-285088-krim73zt 42 38 heated heat VBN cord-285088-krim73zt 42 39 at at IN cord-285088-krim73zt 42 40 the the DT cord-285088-krim73zt 42 41 optimal optimal JJ cord-285088-krim73zt 42 42 temperature temperature NN cord-285088-krim73zt 42 43 for for IN cord-285088-krim73zt 42 44 60 60 CD cord-285088-krim73zt 42 45 min min NN cord-285088-krim73zt 42 46 in in IN cord-285088-krim73zt 42 47 a a DT cord-285088-krim73zt 42 48 StepOneTM StepOneTM NNP cord-285088-krim73zt 42 49 System system NN cord-285088-krim73zt 42 50 ( ( -LRB- cord-285088-krim73zt 42 51 Applied Applied NNP cord-285088-krim73zt 42 52 Biosystems Biosystems NNP cord-285088-krim73zt 42 53 , , , cord-285088-krim73zt 42 54 Foster Foster NNP cord-285088-krim73zt 42 55 City City NNP cord-285088-krim73zt 42 56 , , , cord-285088-krim73zt 42 57 CA CA NNP cord-285088-krim73zt 42 58 , , , cord-285088-krim73zt 42 59 USA USA NNP cord-285088-krim73zt 42 60 ) ) -RRB- cord-285088-krim73zt 42 61 or or CC cord-285088-krim73zt 42 62 in in IN cord-285088-krim73zt 42 63 a a DT cord-285088-krim73zt 42 64 water water NN cord-285088-krim73zt 42 65 bath bath NN cord-285088-krim73zt 42 66 , , , cord-285088-krim73zt 42 67 when when WRB cord-285088-krim73zt 42 68 water water NN cord-285088-krim73zt 42 69 bath bath NN cord-285088-krim73zt 42 70 was be VBD cord-285088-krim73zt 42 71 used use VBN cord-285088-krim73zt 42 72 , , , cord-285088-krim73zt 42 73 the the DT cord-285088-krim73zt 42 74 reaction reaction NN cord-285088-krim73zt 42 75 tube tube NN cord-285088-krim73zt 42 76 was be VBD cord-285088-krim73zt 42 77 sunk sink VBN cord-285088-krim73zt 42 78 into into IN cord-285088-krim73zt 42 79 water water NN cord-285088-krim73zt 42 80 . . . cord-285088-krim73zt 42 81 _SP cord-285088-krim73zt 43 1 The the DT cord-285088-krim73zt 43 2 stability stability NN cord-285088-krim73zt 43 3 and and CC cord-285088-krim73zt 43 4 the the DT cord-285088-krim73zt 43 5 repeatability repeatability NN cord-285088-krim73zt 43 6 of of IN cord-285088-krim73zt 43 7 the the DT cord-285088-krim73zt 43 8 real real JJ cord-285088-krim73zt 43 9 - - HYPH cord-285088-krim73zt 43 10 time time NN cord-285088-krim73zt 43 11 LAMP lamp NN cord-285088-krim73zt 43 12 system system NN cord-285088-krim73zt 43 13 were be VBD cord-285088-krim73zt 43 14 tested test VBN cord-285088-krim73zt 43 15 with with IN cord-285088-krim73zt 43 16 one one CD cord-285088-krim73zt 43 17 - - HYPH cord-285088-krim73zt 43 18 month month NN cord-285088-krim73zt 43 19 interval interval NN cord-285088-krim73zt 43 20 . . . cord-285088-krim73zt 44 1 The the DT cord-285088-krim73zt 44 2 test test NN cord-285088-krim73zt 44 3 was be VBD cord-285088-krim73zt 44 4 carried carry VBN cord-285088-krim73zt 44 5 out out RP cord-285088-krim73zt 44 6 at at IN cord-285088-krim73zt 44 7 the the DT cord-285088-krim73zt 44 8 optimized optimize VBN cord-285088-krim73zt 44 9 temperature temperature NN cord-285088-krim73zt 44 10 determined determine VBN cord-285088-krim73zt 44 11 as as IN cord-285088-krim73zt 44 12 described describe VBN cord-285088-krim73zt 44 13 above above RB cord-285088-krim73zt 44 14 with with IN cord-285088-krim73zt 44 15 four four CD cord-285088-krim73zt 44 16 positive positive JJ cord-285088-krim73zt 44 17 controls control NNS cord-285088-krim73zt 44 18 ( ( -LRB- cord-285088-krim73zt 44 19 1 1 CD cord-285088-krim73zt 44 20 pg pg NN cord-285088-krim73zt 44 21 pUC57-N puc57-n CC cord-285088-krim73zt 44 22 DNA DNA NNP cord-285088-krim73zt 44 23 ) ) -RRB- cord-285088-krim73zt 44 24 and and CC cord-285088-krim73zt 44 25 four four CD cord-285088-krim73zt 44 26 negative negative JJ cord-285088-krim73zt 44 27 controls control NNS cord-285088-krim73zt 44 28 ( ( -LRB- cord-285088-krim73zt 44 29 ddH ddh JJ cord-285088-krim73zt 44 30 2 2 CD cord-285088-krim73zt 44 31 O o NN cord-285088-krim73zt 44 32 ) ) -RRB- cord-285088-krim73zt 44 33 in in IN cord-285088-krim73zt 44 34 a a DT cord-285088-krim73zt 44 35 StepOne StepOne NNP cord-285088-krim73zt 44 36 TM TM NNP cord-285088-krim73zt 44 37 System System NNP cord-285088-krim73zt 44 38 ( ( -LRB- cord-285088-krim73zt 44 39 Applied Applied NNP cord-285088-krim73zt 44 40 Biosystems Biosystems NNP cord-285088-krim73zt 44 41 , , , cord-285088-krim73zt 44 42 Foster Foster NNP cord-285088-krim73zt 44 43 City City NNP cord-285088-krim73zt 44 44 , , , cord-285088-krim73zt 44 45 CA CA NNP cord-285088-krim73zt 44 46 , , , cord-285088-krim73zt 44 47 USA USA NNP cord-285088-krim73zt 44 48 ) ) -RRB- cord-285088-krim73zt 44 49 . . . cord-285088-krim73zt 45 1 For for IN cord-285088-krim73zt 45 2 the the DT cord-285088-krim73zt 45 3 RT RT NNP cord-285088-krim73zt 45 4 - - HYPH cord-285088-krim73zt 45 5 LAMP LAMP NNP cord-285088-krim73zt 45 6 reaction reaction NN cord-285088-krim73zt 45 7 , , , cord-285088-krim73zt 45 8 as as IN cord-285088-krim73zt 45 9 shown show VBN cord-285088-krim73zt 45 10 in in IN cord-285088-krim73zt 45 11 Figure figure NN cord-285088-krim73zt 45 12 1 1 CD cord-285088-krim73zt 45 13 , , , cord-285088-krim73zt 45 14 all all DT cord-285088-krim73zt 45 15 positive positive JJ cord-285088-krim73zt 45 16 controls control NNS cord-285088-krim73zt 45 17 ( ( -LRB- cord-285088-krim73zt 45 18 with with IN cord-285088-krim73zt 45 19 pUC57-N puc57-n CD cord-285088-krim73zt 45 20 DNA DNA NNP cord-285088-krim73zt 45 21 as as IN cord-285088-krim73zt 45 22 template template NN cord-285088-krim73zt 45 23 ) ) -RRB- cord-285088-krim73zt 45 24 had have VBD cord-285088-krim73zt 45 25 amplification amplification NN cord-285088-krim73zt 45 26 , , , cord-285088-krim73zt 45 27 and and CC cord-285088-krim73zt 45 28 the the DT cord-285088-krim73zt 45 29 results result NNS cord-285088-krim73zt 45 30 of of IN cord-285088-krim73zt 45 31 all all DT cord-285088-krim73zt 45 32 negative negative JJ cord-285088-krim73zt 45 33 controls control NNS cord-285088-krim73zt 45 34 ( ( -LRB- cord-285088-krim73zt 45 35 DNA dna NN cord-285088-krim73zt 45 36 template template NN cord-285088-krim73zt 45 37 substituted substitute VBN cord-285088-krim73zt 45 38 by by IN cord-285088-krim73zt 45 39 ddH ddh JJ cord-285088-krim73zt 45 40 2 2 CD cord-285088-krim73zt 45 41 O o NN cord-285088-krim73zt 45 42 ) ) -RRB- cord-285088-krim73zt 45 43 were be VBD cord-285088-krim73zt 45 44 negative negative JJ cord-285088-krim73zt 45 45 at at IN cord-285088-krim73zt 45 46 55 55 CD cord-285088-krim73zt 45 47 ° ° NN cord-285088-krim73zt 45 48 C c NN cord-285088-krim73zt 45 49 , , , cord-285088-krim73zt 45 50 57 57 CD cord-285088-krim73zt 45 51 ° ° NN cord-285088-krim73zt 45 52 C c NN cord-285088-krim73zt 45 53 , , , cord-285088-krim73zt 45 54 59 59 CD cord-285088-krim73zt 45 55 ° ° NN cord-285088-krim73zt 45 56 C c NN cord-285088-krim73zt 45 57 and and CC cord-285088-krim73zt 45 58 91 91 CD cord-285088-krim73zt 45 59 ° ° NN cord-285088-krim73zt 45 60 C c NN cord-285088-krim73zt 45 61 , , , cord-285088-krim73zt 45 62 however however RB cord-285088-krim73zt 45 63 , , , cord-285088-krim73zt 45 64 there there EX cord-285088-krim73zt 45 65 was be VBD cord-285088-krim73zt 45 66 no no DT cord-285088-krim73zt 45 67 significant significant JJ cord-285088-krim73zt 45 68 difference difference NN cord-285088-krim73zt 45 69 in in IN cord-285088-krim73zt 45 70 the the DT cord-285088-krim73zt 45 71 amplification amplification NN cord-285088-krim73zt 45 72 efficiency efficiency NN cord-285088-krim73zt 45 73 , , , cord-285088-krim73zt 45 74 and and CC cord-285088-krim73zt 45 75 59 59 CD cord-285088-krim73zt 45 76 ° ° NN cord-285088-krim73zt 45 77 C c NN cord-285088-krim73zt 45 78 was be VBD cord-285088-krim73zt 45 79 temporarily temporarily RB cord-285088-krim73zt 45 80 was be VBD cord-285088-krim73zt 45 81 selected select VBN cord-285088-krim73zt 45 82 for for IN cord-285088-krim73zt 45 83 the the DT cord-285088-krim73zt 45 84 subsequent subsequent JJ cord-285088-krim73zt 45 85 experiments experiment NNS cord-285088-krim73zt 45 86 . . . cord-285088-krim73zt 45 87 _SP cord-285088-krim73zt 46 1 The the DT cord-285088-krim73zt 46 2 effect effect NN cord-285088-krim73zt 46 3 of of IN cord-285088-krim73zt 46 4 inhibitors inhibitor NNS cord-285088-krim73zt 46 5 in in IN cord-285088-krim73zt 46 6 blood blood NN cord-285088-krim73zt 46 7 samples sample NNS cord-285088-krim73zt 46 8 on on IN cord-285088-krim73zt 46 9 the the DT cord-285088-krim73zt 46 10 amplification amplification NN cord-285088-krim73zt 46 11 efficiency efficiency NN cord-285088-krim73zt 46 12 had have VBD cord-285088-krim73zt 46 13 been be VBN cord-285088-krim73zt 46 14 determined determine VBN cord-285088-krim73zt 46 15 , , , cord-285088-krim73zt 46 16 as as IN cord-285088-krim73zt 46 17 Figure figure NN cord-285088-krim73zt 46 18 2 2 CD cord-285088-krim73zt 46 19 indicated indicate VBD cord-285088-krim73zt 46 20 , , , cord-285088-krim73zt 46 21 when when WRB cord-285088-krim73zt 46 22 the the DT cord-285088-krim73zt 46 23 real real JJ cord-285088-krim73zt 46 24 - - HYPH cord-285088-krim73zt 46 25 time time NN cord-285088-krim73zt 46 26 RT rt NN cord-285088-krim73zt 46 27 - - HYPH cord-285088-krim73zt 46 28 LAMP LAMP NNP cord-285088-krim73zt 46 29 reaction reaction NN cord-285088-krim73zt 46 30 system system NN cord-285088-krim73zt 46 31 was be VBD cord-285088-krim73zt 46 32 added add VBN cord-285088-krim73zt 46 33 with with IN cord-285088-krim73zt 46 34 10 10 CD cord-285088-krim73zt 46 35 µL µL NNP cord-285088-krim73zt 46 36 blood blood NN cord-285088-krim73zt 46 37 samples sample NNS cord-285088-krim73zt 46 38 dissolved dissolve VBN cord-285088-krim73zt 46 39 in in IN cord-285088-krim73zt 46 40 10 10 CD cord-285088-krim73zt 46 41 µL µl XX cord-285088-krim73zt 47 1 2019-nCoV 2019-ncov LS cord-285088-krim73zt 47 2 - - HYPH cord-285088-krim73zt 47 3 Fast fast JJ cord-285088-krim73zt 47 4 - - HYPH cord-285088-krim73zt 47 5 Sample sample NN cord-285088-krim73zt 47 6 Nucleic Nucleic NNS cord-285088-krim73zt 47 7 Acid acid NN cord-285088-krim73zt 47 8 Releasing release VBG cord-285088-krim73zt 47 9 Agent Agent NNP cord-285088-krim73zt 47 10 ( ( -LRB- cord-285088-krim73zt 47 11 Hunan Hunan NNP cord-285088-krim73zt 47 12 Shengxiang Shengxiang NNP cord-285088-krim73zt 47 13 Biology Biology NNP cord-285088-krim73zt 47 14 Technology Technology NNP cord-285088-krim73zt 47 15 Co. Co. NNP cord-285088-krim73zt 47 16 , , , cord-285088-krim73zt 47 17 Ltd Ltd NNP cord-285088-krim73zt 47 18 , , , cord-285088-krim73zt 47 19 China China NNP cord-285088-krim73zt 47 20 ) ) -RRB- cord-285088-krim73zt 48 1 , , , cord-285088-krim73zt 48 2 there there EX cord-285088-krim73zt 48 3 was be VBD cord-285088-krim73zt 48 4 no no DT cord-285088-krim73zt 48 5 amplification amplification NN cord-285088-krim73zt 48 6 ; ; . cord-285088-krim73zt 49 1 when when WRB cord-285088-krim73zt 49 2 7.5 7.5 CD cord-285088-krim73zt 49 3 µL µl IN cord-285088-krim73zt 49 4 blood blood NN cord-285088-krim73zt 49 5 samples sample NNS cord-285088-krim73zt 49 6 and and CC cord-285088-krim73zt 49 7 10 10 CD cord-285088-krim73zt 49 8 µL µl CD cord-285088-krim73zt 49 9 2019-nCoV 2019-ncov CD cord-285088-krim73zt 49 10 - - HYPH cord-285088-krim73zt 49 11 Fast fast JJ cord-285088-krim73zt 49 12 - - HYPH cord-285088-krim73zt 49 13 Sample sample NN cord-285088-krim73zt 49 14 Nucleic nucleic NN cord-285088-krim73zt 49 15 Acid acid NN cord-285088-krim73zt 49 16 Releasing release VBG cord-285088-krim73zt 49 17 Agent Agent NNP cord-285088-krim73zt 49 18 added add VBD cord-285088-krim73zt 49 19 , , , cord-285088-krim73zt 49 20 there there EX cord-285088-krim73zt 49 21 was be VBD cord-285088-krim73zt 49 22 slight slight JJ cord-285088-krim73zt 49 23 amplification amplification NN cord-285088-krim73zt 49 24 ; ; : cord-285088-krim73zt 49 25 when when WRB cord-285088-krim73zt 49 26 5µL 5µl CD cord-285088-krim73zt 49 27 or or CC cord-285088-krim73zt 49 28 2.5 2.5 CD cord-285088-krim73zt 49 29 µL µl IN cord-285088-krim73zt 49 30 blood blood NN cord-285088-krim73zt 49 31 samples sample NNS cord-285088-krim73zt 49 32 dissolved dissolve VBN cord-285088-krim73zt 49 33 in in IN cord-285088-krim73zt 50 1 10 10 CD cord-285088-krim73zt 50 2 µL µl CD cord-285088-krim73zt 50 3 2019-nCoV 2019-ncov CD cord-285088-krim73zt 50 4 - - HYPH cord-285088-krim73zt 50 5 Fast fast JJ cord-285088-krim73zt 50 6 - - HYPH cord-285088-krim73zt 50 7 Sample sample NN cord-285088-krim73zt 50 8 Nucleic nucleic NN cord-285088-krim73zt 50 9 Acid acid NN cord-285088-krim73zt 50 10 Releasing release VBG cord-285088-krim73zt 50 11 Agent Agent NNP cord-285088-krim73zt 50 12 added add VBD cord-285088-krim73zt 50 13 , , , cord-285088-krim73zt 50 14 there there EX cord-285088-krim73zt 50 15 was be VBD cord-285088-krim73zt 50 16 acceptable acceptable JJ cord-285088-krim73zt 50 17 effect effect NN cord-285088-krim73zt 50 18 on on IN cord-285088-krim73zt 50 19 the the DT cord-285088-krim73zt 50 20 real real JJ cord-285088-krim73zt 50 21 - - HYPH cord-285088-krim73zt 50 22 time time NN cord-285088-krim73zt 50 23 RT rt NN cord-285088-krim73zt 50 24 - - HYPH cord-285088-krim73zt 50 25 LAMP LAMP NNP cord-285088-krim73zt 50 26 reaction reaction NN cord-285088-krim73zt 50 27 . . . cord-285088-krim73zt 51 1 Therefore therefore RB cord-285088-krim73zt 51 2 , , , cord-285088-krim73zt 51 3 the the DT cord-285088-krim73zt 51 4 blood blood NN cord-285088-krim73zt 51 5 sample sample NN cord-285088-krim73zt 51 6 volume volume NN cord-285088-krim73zt 51 7 of of IN cord-285088-krim73zt 51 8 5µL 5µl CD cord-285088-krim73zt 51 9 was be VBD cord-285088-krim73zt 51 10 selected select VBN cord-285088-krim73zt 51 11 for for IN cord-285088-krim73zt 51 12 50 50 CD cord-285088-krim73zt 51 13 µL µl CD cord-285088-krim73zt 51 14 one one CD cord-285088-krim73zt 51 15 - - HYPH cord-285088-krim73zt 51 16 pot pot NN cord-285088-krim73zt 51 17 real real JJ cord-285088-krim73zt 51 18 - - HYPH cord-285088-krim73zt 51 19 time time NN cord-285088-krim73zt 51 20 or or CC cord-285088-krim73zt 51 21 visual visual JJ cord-285088-krim73zt 51 22 RT rt NN cord-285088-krim73zt 51 23 - - HYPH cord-285088-krim73zt 51 24 LAMP LAMP NNP cord-285088-krim73zt 51 25 reaction reaction NN cord-285088-krim73zt 51 26 . . . cord-285088-krim73zt 52 1 The the DT cord-285088-krim73zt 52 2 detection detection NN cord-285088-krim73zt 52 3 limits limit NNS cord-285088-krim73zt 52 4 of of IN cord-285088-krim73zt 52 5 the the DT cord-285088-krim73zt 52 6 one one CD cord-285088-krim73zt 52 7 - - HYPH cord-285088-krim73zt 52 8 pot pot NN cord-285088-krim73zt 52 9 real real JJ cord-285088-krim73zt 52 10 - - HYPH cord-285088-krim73zt 52 11 time time NN cord-285088-krim73zt 52 12 RT RT NNP cord-285088-krim73zt 52 13 - - HYPH cord-285088-krim73zt 52 14 LAMP LAMP NNP cord-285088-krim73zt 52 15 assay assay NN cord-285088-krim73zt 52 16 and and CC cord-285088-krim73zt 52 17 the the DT cord-285088-krim73zt 52 18 one one CD cord-285088-krim73zt 52 19 - - HYPH cord-285088-krim73zt 52 20 pot pot NN cord-285088-krim73zt 52 21 visual visual JJ cord-285088-krim73zt 52 22 RT rt NN cord-285088-krim73zt 52 23 - - HYPH cord-285088-krim73zt 52 24 LAMP LAMP NNP cord-285088-krim73zt 52 25 assay assay NN cord-285088-krim73zt 52 26 were be VBD cord-285088-krim73zt 52 27 determined determine VBN cord-285088-krim73zt 52 28 using use VBG cord-285088-krim73zt 52 29 pUC57-N puc57-n JJ cord-285088-krim73zt 52 30 DNA dna NN cord-285088-krim73zt 52 31 ranging range VBG cord-285088-krim73zt 52 32 from from IN cord-285088-krim73zt 52 33 2 2 CD cord-285088-krim73zt 52 34 - - SYM cord-285088-krim73zt 52 35 0.02 0.02 CD cord-285088-krim73zt 52 36 fg fg NN cord-285088-krim73zt 52 37 at at IN cord-285088-krim73zt 52 38 59 59 CD cord-285088-krim73zt 52 39 ° ° NN cord-285088-krim73zt 52 40 C c NN cord-285088-krim73zt 52 41 for for IN cord-285088-krim73zt 52 42 60 60 CD cord-285088-krim73zt 52 43 min min NN cord-285088-krim73zt 52 44 in in IN cord-285088-krim73zt 52 45 a a DT cord-285088-krim73zt 52 46 StepOneTM StepOneTM NNP cord-285088-krim73zt 52 47 System system NN cord-285088-krim73zt 52 48 ( ( -LRB- cord-285088-krim73zt 52 49 Applied Applied NNP cord-285088-krim73zt 52 50 Biosystems Biosystems NNP cord-285088-krim73zt 52 51 , , , cord-285088-krim73zt 52 52 Foster Foster NNP cord-285088-krim73zt 52 53 City City NNP cord-285088-krim73zt 52 54 , , , cord-285088-krim73zt 52 55 CA CA NNP cord-285088-krim73zt 52 56 , , , cord-285088-krim73zt 52 57 USA USA NNP cord-285088-krim73zt 52 58 ) ) -RRB- cord-285088-krim73zt 52 59 or or CC cord-285088-krim73zt 52 60 in in IN cord-285088-krim73zt 52 61 a a DT cord-285088-krim73zt 52 62 water water NN cord-285088-krim73zt 52 63 bath bath NN cord-285088-krim73zt 52 64 . . . cord-285088-krim73zt 53 1 The the DT cord-285088-krim73zt 53 2 detection detection NN cord-285088-krim73zt 53 3 limits limit NNS cord-285088-krim73zt 53 4 of of IN cord-285088-krim73zt 53 5 both both CC cord-285088-krim73zt 53 6 the the DT cord-285088-krim73zt 53 7 one one CD cord-285088-krim73zt 53 8 - - HYPH cord-285088-krim73zt 53 9 pot pot NN cord-285088-krim73zt 53 10 real real JJ cord-285088-krim73zt 53 11 - - HYPH cord-285088-krim73zt 53 12 time time NN cord-285088-krim73zt 53 13 RT RT NNP cord-285088-krim73zt 53 14 - - HYPH cord-285088-krim73zt 53 15 LAMP LAMP NNP cord-285088-krim73zt 53 16 assay assay NN cord-285088-krim73zt 53 17 and and CC cord-285088-krim73zt 53 18 the the DT cord-285088-krim73zt 53 19 one one CD cord-285088-krim73zt 53 20 - - HYPH cord-285088-krim73zt 53 21 pot pot NN cord-285088-krim73zt 53 22 visual visual JJ cord-285088-krim73zt 53 23 RT rt NN cord-285088-krim73zt 53 24 - - HYPH cord-285088-krim73zt 53 25 LAMP LAMP NNP cord-285088-krim73zt 53 26 assay assay NN cord-285088-krim73zt 53 27 were be VBD cord-285088-krim73zt 53 28 found find VBN cord-285088-krim73zt 53 29 to to TO cord-285088-krim73zt 53 30 be be VB cord-285088-krim73zt 53 31 ≥ ≥ CD cord-285088-krim73zt 53 32 0.2 0.2 CD cord-285088-krim73zt 53 33 fg fg NN cord-285088-krim73zt 53 34 pUC57-N puc57-n CC cord-285088-krim73zt 53 35 DNA dna NN cord-285088-krim73zt 53 36 ( ( -LRB- cord-285088-krim73zt 53 37 Figure figure NN cord-285088-krim73zt 53 38 3 3 CD cord-285088-krim73zt 53 39 ) ) -RRB- cord-285088-krim73zt 53 40 , , , cord-285088-krim73zt 53 41 which which WDT cord-285088-krim73zt 53 42 was be VBD cord-285088-krim73zt 53 43 equivalent equivalent JJ cord-285088-krim73zt 53 44 to to IN cord-285088-krim73zt 53 45 ≥ ≥ CD cord-285088-krim73zt 53 46 6 6 CD cord-285088-krim73zt 53 47 copies copy NNS cord-285088-krim73zt 53 48 . . . cord-285088-krim73zt 53 49 _SP cord-285088-krim73zt 54 1 The the DT cord-285088-krim73zt 54 2 stability stability NN cord-285088-krim73zt 54 3 and and CC cord-285088-krim73zt 54 4 the the DT cord-285088-krim73zt 54 5 repeatability repeatability NN cord-285088-krim73zt 54 6 of of IN cord-285088-krim73zt 54 7 the the DT cord-285088-krim73zt 54 8 real real JJ cord-285088-krim73zt 54 9 - - HYPH cord-285088-krim73zt 54 10 time time NN cord-285088-krim73zt 54 11 LAMP lamp NN cord-285088-krim73zt 54 12 system system NN cord-285088-krim73zt 54 13 were be VBD cord-285088-krim73zt 54 14 tested test VBN cord-285088-krim73zt 54 15 with with IN cord-285088-krim73zt 54 16 one one CD cord-285088-krim73zt 54 17 - - HYPH cord-285088-krim73zt 54 18 month month NN cord-285088-krim73zt 54 19 interval interval NN cord-285088-krim73zt 54 20 . . . cord-285088-krim73zt 55 1 As as IN cord-285088-krim73zt 55 2 shown show VBN cord-285088-krim73zt 55 3 in in IN cord-285088-krim73zt 55 4 Figure Figure NNP cord-285088-krim73zt 55 5 4 4 CD cord-285088-krim73zt 55 6 , , , cord-285088-krim73zt 55 7 the the DT cord-285088-krim73zt 55 8 reactions reaction NNS cord-285088-krim73zt 55 9 of of IN cord-285088-krim73zt 55 10 four four CD cord-285088-krim73zt 55 11 positive positive JJ cord-285088-krim73zt 55 12 controls control NNS cord-285088-krim73zt 55 13 were be VBD cord-285088-krim73zt 55 14 positive positive JJ cord-285088-krim73zt 55 15 with with IN cord-285088-krim73zt 55 16 the the DT cord-285088-krim73zt 55 17 same same JJ cord-285088-krim73zt 55 18 amplification amplification NN cord-285088-krim73zt 55 19 plot plot NN cord-285088-krim73zt 55 20 and and CC cord-285088-krim73zt 55 21 melt melt VB cord-285088-krim73zt 55 22 curve curve NN cord-285088-krim73zt 55 23 , , , cord-285088-krim73zt 55 24 while while IN cord-285088-krim73zt 55 25 the the DT cord-285088-krim73zt 55 26 reactions reaction NNS cord-285088-krim73zt 55 27 of of IN cord-285088-krim73zt 55 28 all all DT cord-285088-krim73zt 55 29 negative negative JJ cord-285088-krim73zt 55 30 controls control NNS cord-285088-krim73zt 55 31 were be VBD cord-285088-krim73zt 55 32 negative negative JJ cord-285088-krim73zt 55 33 with with IN cord-285088-krim73zt 55 34 the the DT cord-285088-krim73zt 55 35 same same JJ cord-285088-krim73zt 55 36 melt melt NN cord-285088-krim73zt 55 37 curve curve NN cord-285088-krim73zt 55 38 . . . cord-285088-krim73zt 56 1 This this DT cord-285088-krim73zt 56 2 demonstrated demonstrate VBD cord-285088-krim73zt 56 3 that that IN cord-285088-krim73zt 56 4 the the DT cord-285088-krim73zt 56 5 newly newly RB cord-285088-krim73zt 56 6 established establish VBN cord-285088-krim73zt 56 7 real real JJ cord-285088-krim73zt 56 8 - - HYPH cord-285088-krim73zt 56 9 time time NN cord-285088-krim73zt 56 10 LAMP lamp NN cord-285088-krim73zt 56 11 assay assay NN cord-285088-krim73zt 56 12 was be VBD cord-285088-krim73zt 56 13 robust robust JJ cord-285088-krim73zt 56 14 and and CC cord-285088-krim73zt 56 15 repeatable repeatable JJ cord-285088-krim73zt 56 16 . . . cord-285088-krim73zt 56 17 _SP cord-285088-krim73zt 57 1 The the DT cord-285088-krim73zt 57 2 one one CD cord-285088-krim73zt 57 3 - - HYPH cord-285088-krim73zt 57 4 pot pot NN cord-285088-krim73zt 57 5 real real JJ cord-285088-krim73zt 57 6 - - HYPH cord-285088-krim73zt 57 7 time time NN cord-285088-krim73zt 57 8 RT RT NNP cord-285088-krim73zt 57 9 - - HYPH cord-285088-krim73zt 57 10 LAMP LAMP NNP cord-285088-krim73zt 57 11 assay assay NN cord-285088-krim73zt 57 12 and and CC cord-285088-krim73zt 57 13 one one CD cord-285088-krim73zt 57 14 - - HYPH cord-285088-krim73zt 57 15 pot pot NN cord-285088-krim73zt 57 16 visual visual JJ cord-285088-krim73zt 57 17 RT rt NN cord-285088-krim73zt 57 18 - - HYPH cord-285088-krim73zt 57 19 LAMP LAMP NNP cord-285088-krim73zt 57 20 assay assay NN cord-285088-krim73zt 57 21 for for IN cord-285088-krim73zt 57 22 detection detection NN cord-285088-krim73zt 57 23 of of IN cord-285088-krim73zt 57 24 COVID-19 COVID-19 NNP cord-285088-krim73zt 57 25 were be VBD cord-285088-krim73zt 57 26 established establish VBN cord-285088-krim73zt 57 27 in in IN cord-285088-krim73zt 57 28 the the DT cord-285088-krim73zt 57 29 study study NN cord-285088-krim73zt 57 30 , , , cord-285088-krim73zt 57 31 and and CC cord-285088-krim73zt 57 32 the the DT cord-285088-krim73zt 57 33 detection detection NN cord-285088-krim73zt 57 34 limit limit NN cord-285088-krim73zt 57 35 was be VBD cord-285088-krim73zt 57 36 ≥ ≥ CD cord-285088-krim73zt 57 37 6 6 CD cord-285088-krim73zt 57 38 copies copy NNS cord-285088-krim73zt 57 39 . . . cord-285088-krim73zt 58 1 Although although IN cord-285088-krim73zt 58 2 the the DT cord-285088-krim73zt 58 3 specificity specificity NN cord-285088-krim73zt 58 4 of of IN cord-285088-krim73zt 58 5 the the DT cord-285088-krim73zt 58 6 established establish VBN cord-285088-krim73zt 58 7 RT RT NNP cord-285088-krim73zt 58 8 - - HYPH cord-285088-krim73zt 58 9 LAMP LAMP NNP cord-285088-krim73zt 58 10 assays assay NNS cord-285088-krim73zt 58 11 had have VBD cord-285088-krim73zt 58 12 been be VBN cord-285088-krim73zt 58 13 not not RB cord-285088-krim73zt 58 14 determined determine VBN cord-285088-krim73zt 58 15 , , , cord-285088-krim73zt 58 16 they -PRON- PRP cord-285088-krim73zt 58 17 were be VBD cord-285088-krim73zt 58 18 still still RB cord-285088-krim73zt 58 19 considered consider VBN cord-285088-krim73zt 58 20 to to TO cord-285088-krim73zt 58 21 be be VB cord-285088-krim73zt 58 22 highly highly RB cord-285088-krim73zt 58 23 specific specific JJ cord-285088-krim73zt 58 24 for for IN cord-285088-krim73zt 58 25 following follow VBG cord-285088-krim73zt 58 26 reason reason NN cord-285088-krim73zt 58 27 . . . cord-285088-krim73zt 59 1 Upon upon IN cord-285088-krim73zt 59 2 alignment alignment NN cord-285088-krim73zt 59 3 in in IN cord-285088-krim73zt 59 4 DNA DNA NNP cord-285088-krim73zt 59 5 Data Data NNP cord-285088-krim73zt 59 6 Bank Bank NNP cord-285088-krim73zt 59 7 of of IN cord-285088-krim73zt 59 8 Japan Japan NNP cord-285088-krim73zt 59 9 , , , cord-285088-krim73zt 59 10 the the DT cord-285088-krim73zt 59 11 target target NN cord-285088-krim73zt 59 12 sequence sequence NN cord-285088-krim73zt 59 13 of of IN cord-285088-krim73zt 59 14 established establish VBN cord-285088-krim73zt 59 15 RT RT NNP cord-285088-krim73zt 59 16 - - HYPH cord-285088-krim73zt 59 17 LAMP LAMP NNP cord-285088-krim73zt 59 18 assays assay NNS cord-285088-krim73zt 59 19 had have VBD cord-285088-krim73zt 59 20 100 100 CD cord-285088-krim73zt 59 21 % % NN cord-285088-krim73zt 59 22 identities identity NNS cord-285088-krim73zt 59 23 ( ( -LRB- cord-285088-krim73zt 59 24 233/233 233/233 CD cord-285088-krim73zt 59 25 ) ) -RRB- cord-285088-krim73zt 59 26 with with IN cord-285088-krim73zt 59 27 that that DT cord-285088-krim73zt 59 28 of of IN cord-285088-krim73zt 59 29 35 35 CD cord-285088-krim73zt 60 1 COVID-19 covid-19 JJ cord-285088-krim73zt 60 2 strains strain NNS cord-285088-krim73zt 60 3 , , , cord-285088-krim73zt 60 4 and and CC cord-285088-krim73zt 60 5 had have VBD cord-285088-krim73zt 60 6 88 88 CD cord-285088-krim73zt 60 7 % % NN cord-285088-krim73zt 60 8 identities identity NNS cord-285088-krim73zt 60 9 ( ( -LRB- cord-285088-krim73zt 60 10 207/233 207/233 CD cord-285088-krim73zt 60 11 ) ) -RRB- cord-285088-krim73zt 60 12 with with IN cord-285088-krim73zt 60 13 that that DT cord-285088-krim73zt 60 14 of of IN cord-285088-krim73zt 60 15 52 52 CD cord-285088-krim73zt 60 16 congeneric congeneric JJ cord-285088-krim73zt 60 17 SARS SARS NNP cord-285088-krim73zt 60 18 Coronavirus Coronavirus NNP cord-285088-krim73zt 60 19 strains strain NNS cord-285088-krim73zt 60 20 , , , cord-285088-krim73zt 60 21 among among IN cord-285088-krim73zt 60 22 which which WDT cord-285088-krim73zt 60 23 there there EX cord-285088-krim73zt 60 24 were be VBD cord-285088-krim73zt 60 25 12 12 CD cord-285088-krim73zt 60 26 bp bp NNS cord-285088-krim73zt 60 27 on on IN cord-285088-krim73zt 60 28 the the DT cord-285088-krim73zt 60 29 RT RT NNP cord-285088-krim73zt 60 30 - - HYPH cord-285088-krim73zt 60 31 LAMP LAMP NNP cord-285088-krim73zt 60 32 primers primer NNS cord-285088-krim73zt 60 33 , , , cord-285088-krim73zt 60 34 it -PRON- PRP cord-285088-krim73zt 60 35 was be VBD cord-285088-krim73zt 60 36 reported report VBN cord-285088-krim73zt 60 37 that that IN cord-285088-krim73zt 60 38 3 3 CD cord-285088-krim73zt 60 39 bp bp NNP cord-285088-krim73zt 60 40 primer primer NN cord-285088-krim73zt 60 41 - - HYPH cord-285088-krim73zt 60 42 template template NN cord-285088-krim73zt 60 43 mismatches mismatch NNS cord-285088-krim73zt 60 44 extend extend VBP cord-285088-krim73zt 60 45 the the DT cord-285088-krim73zt 60 46 detection detection NN cord-285088-krim73zt 60 47 time time NN cord-285088-krim73zt 60 48 from from IN cord-285088-krim73zt 60 49 21 21 CD cord-285088-krim73zt 60 50 min min NN cord-285088-krim73zt 60 51 to to IN cord-285088-krim73zt 60 52 47 47 CD cord-285088-krim73zt 60 53 min min NN cord-285088-krim73zt 60 54 10 10 CD cord-285088-krim73zt 60 55 , , , cord-285088-krim73zt 60 56 therefore therefore RB cord-285088-krim73zt 60 57 , , , cord-285088-krim73zt 60 58 the the DT cord-285088-krim73zt 60 59 established establish VBN cord-285088-krim73zt 60 60 RT RT NNP cord-285088-krim73zt 60 61 - - HYPH cord-285088-krim73zt 60 62 LAMP LAMP NNP cord-285088-krim73zt 60 63 assays assay NNS cord-285088-krim73zt 60 64 were be VBD cord-285088-krim73zt 60 65 theoretically theoretically RB cord-285088-krim73zt 60 66 highly highly RB cord-285088-krim73zt 60 67 specific specific JJ cord-285088-krim73zt 60 68 . . . cord-285088-krim73zt 61 1 The the DT cord-285088-krim73zt 61 2 Bst Bst NNP cord-285088-krim73zt 61 3 DNA DNA NNP cord-285088-krim73zt 61 4 / / SYM cord-285088-krim73zt 61 5 RNA RNA NNP cord-285088-krim73zt 61 6 Polymerase Polymerase NNP cord-285088-krim73zt 61 7 3.0 3.0 CD cord-285088-krim73zt 62 1 ( ( -LRB- cord-285088-krim73zt 62 2 New New NNP cord-285088-krim73zt 62 3 England England NNP cord-285088-krim73zt 62 4 Biolabs Biolabs NNP cord-285088-krim73zt 62 5 , , , cord-285088-krim73zt 62 6 Inc. Inc. NNP cord-285088-krim73zt 62 7 , , , cord-285088-krim73zt 62 8 MA MA NNP cord-285088-krim73zt 62 9 , , , cord-285088-krim73zt 62 10 USA USA NNP cord-285088-krim73zt 62 11 ) ) -RRB- cord-285088-krim73zt 62 12 had have VBD cord-285088-krim73zt 62 13 been be VBN cord-285088-krim73zt 62 14 used use VBN cord-285088-krim73zt 62 15 in in IN cord-285088-krim73zt 62 16 the the DT cord-285088-krim73zt 62 17 study study NN cord-285088-krim73zt 62 18 . . . cord-285088-krim73zt 63 1 The the DT cord-285088-krim73zt 63 2 enzyme enzyme NN cord-285088-krim73zt 63 3 has have VBZ cord-285088-krim73zt 63 4 faborable faborable JJ cord-285088-krim73zt 63 5 perforance perforance NN cord-285088-krim73zt 63 6 of of IN cord-285088-krim73zt 63 7 both both CC cord-285088-krim73zt 63 8 amplification amplification NN cord-285088-krim73zt 63 9 and and CC cord-285088-krim73zt 63 10 reverse reverse VB cord-285088-krim73zt 63 11 transcription transcription NN cord-285088-krim73zt 63 12 activity activity NN cord-285088-krim73zt 63 13 , , , cord-285088-krim73zt 63 14 so so IN cord-285088-krim73zt 63 15 it -PRON- PRP cord-285088-krim73zt 63 16 can can MD cord-285088-krim73zt 63 17 use use VB cord-285088-krim73zt 63 18 either either CC cord-285088-krim73zt 63 19 DNA DNA NNP cord-285088-krim73zt 63 20 or or CC cord-285088-krim73zt 63 21 RNA RNA NNP cord-285088-krim73zt 63 22 as as IN cord-285088-krim73zt 63 23 template template NN cord-285088-krim73zt 63 24 11 11 CD cord-285088-krim73zt 63 25 , , , cord-285088-krim73zt 63 26 12 12 CD cord-285088-krim73zt 63 27 , , , cord-285088-krim73zt 63 28 no no DT cord-285088-krim73zt 63 29 reverse reverse JJ cord-285088-krim73zt 63 30 transcriptase transcriptase NN cord-285088-krim73zt 63 31 is be VBZ cord-285088-krim73zt 63 32 needed need VBN cord-285088-krim73zt 63 33 , , , cord-285088-krim73zt 63 34 and and CC cord-285088-krim73zt 63 35 the the DT cord-285088-krim73zt 63 36 estalished estalished JJ cord-285088-krim73zt 63 37 assays assay NNS cord-285088-krim73zt 63 38 can can MD cord-285088-krim73zt 63 39 be be VB cord-285088-krim73zt 63 40 directly directly RB cord-285088-krim73zt 63 41 used use VBN cord-285088-krim73zt 63 42 for for IN cord-285088-krim73zt 63 43 detection detection NN cord-285088-krim73zt 63 44 of of IN cord-285088-krim73zt 63 45 the the DT cord-285088-krim73zt 63 46 RNA RNA NNP cord-285088-krim73zt 63 47 virus virus NN cord-285088-krim73zt 63 48 . . . cord-285088-krim73zt 64 1 The the DT cord-285088-krim73zt 64 2 nucleic nucleic JJ cord-285088-krim73zt 64 3 acid acid NN cord-285088-krim73zt 64 4 extraction extraction NN cord-285088-krim73zt 64 5 had have VBD cord-285088-krim73zt 64 6 been be VBN cord-285088-krim73zt 64 7 omitted omit VBN cord-285088-krim73zt 64 8 in in IN cord-285088-krim73zt 64 9 the the DT cord-285088-krim73zt 64 10 established establish VBN cord-285088-krim73zt 64 11 one one CD cord-285088-krim73zt 64 12 - - HYPH cord-285088-krim73zt 64 13 pot pot NN cord-285088-krim73zt 64 14 real real JJ cord-285088-krim73zt 64 15 - - HYPH cord-285088-krim73zt 64 16 time time NN cord-285088-krim73zt 64 17 or or CC cord-285088-krim73zt 64 18 visual visual JJ cord-285088-krim73zt 64 19 RT rt NN cord-285088-krim73zt 64 20 - - HYPH cord-285088-krim73zt 64 21 LAMP LAMP NNP cord-285088-krim73zt 64 22 assays assay NNS cord-285088-krim73zt 64 23 , , , cord-285088-krim73zt 64 24 which which WDT cord-285088-krim73zt 64 25 had have VBD cord-285088-krim73zt 64 26 greatly greatly RB cord-285088-krim73zt 64 27 reduced reduce VBN cord-285088-krim73zt 64 28 the the DT cord-285088-krim73zt 64 29 infection infection NN cord-285088-krim73zt 64 30 risk risk NN cord-285088-krim73zt 64 31 of of IN cord-285088-krim73zt 64 32 the the DT cord-285088-krim73zt 64 33 operators operator NNS cord-285088-krim73zt 64 34 . . . cord-285088-krim73zt 65 1 Furthermore furthermore RB cord-285088-krim73zt 65 2 , , , cord-285088-krim73zt 65 3 it -PRON- PRP cord-285088-krim73zt 65 4 was be VBD cord-285088-krim73zt 65 5 recommended recommend VBN cord-285088-krim73zt 65 6 the the DT cord-285088-krim73zt 65 7 visual visual JJ cord-285088-krim73zt 65 8 RT RT NNP cord-285088-krim73zt 65 9 - - HYPH cord-285088-krim73zt 65 10 LAMP LAMP NNP cord-285088-krim73zt 65 11 assay assay NN cord-285088-krim73zt 65 12 over over IN cord-285088-krim73zt 65 13 the the DT cord-285088-krim73zt 65 14 real real JJ cord-285088-krim73zt 65 15 - - HYPH cord-285088-krim73zt 65 16 time time NN cord-285088-krim73zt 65 17 RT RT NNP cord-285088-krim73zt 65 18 - - HYPH cord-285088-krim73zt 65 19 LAMP LAMP NNP cord-285088-krim73zt 65 20 assay assay NN cord-285088-krim73zt 65 21 , , , cord-285088-krim73zt 65 22 one one CD cord-285088-krim73zt 65 23 of of IN cord-285088-krim73zt 65 24 four four CD cord-285088-krim73zt 65 25 Negative Negative NNP cord-285088-krim73zt 65 26 Controls Controls NNPS cord-285088-krim73zt 65 27 in in IN cord-285088-krim73zt 65 28 above above IN cord-285088-krim73zt 65 29 sensitivity sensitivity NN cord-285088-krim73zt 65 30 determination determination NN cord-285088-krim73zt 65 31 of of IN cord-285088-krim73zt 65 32 the the DT cord-285088-krim73zt 65 33 real real JJ cord-285088-krim73zt 65 34 - - HYPH cord-285088-krim73zt 65 35 time time NN cord-285088-krim73zt 65 36 RT RT NNP cord-285088-krim73zt 65 37 - - HYPH cord-285088-krim73zt 65 38 LAMP LAMP NNP cord-285088-krim73zt 65 39 assay assay NN cord-285088-krim73zt 65 40 had have VBD cord-285088-krim73zt 65 41 amplification amplification NN cord-285088-krim73zt 65 42 , , , cord-285088-krim73zt 65 43 while while IN cord-285088-krim73zt 65 44 all all DT cord-285088-krim73zt 65 45 four four CD cord-285088-krim73zt 65 46 Negative Negative NNP cord-285088-krim73zt 65 47 Controls Controls NNPS cord-285088-krim73zt 65 48 of of IN cord-285088-krim73zt 65 49 the the DT cord-285088-krim73zt 65 50 visual visual JJ cord-285088-krim73zt 65 51 RT RT NNP cord-285088-krim73zt 65 52 - - HYPH cord-285088-krim73zt 65 53 LAMP LAMP NNP cord-285088-krim73zt 65 54 assay assay NN cord-285088-krim73zt 65 55 had have VBD cord-285088-krim73zt 65 56 no no DT cord-285088-krim73zt 65 57 amplification amplification NN cord-285088-krim73zt 65 58 , , , cord-285088-krim73zt 65 59 because because IN cord-285088-krim73zt 65 60 the the DT cord-285088-krim73zt 65 61 aerosol aerosol NN cord-285088-krim73zt 65 62 that that WDT cord-285088-krim73zt 65 63 may may MD cord-285088-krim73zt 65 64 leak leak VB cord-285088-krim73zt 65 65 was be VBD cord-285088-krim73zt 65 66 washed wash VBN cord-285088-krim73zt 65 67 by by IN cord-285088-krim73zt 65 68 water water NN cord-285088-krim73zt 65 69 in in IN cord-285088-krim73zt 65 70 water water NN cord-285088-krim73zt 65 71 bath bath NN cord-285088-krim73zt 65 72 , , , cord-285088-krim73zt 65 73 the the DT cord-285088-krim73zt 65 74 false false JJ cord-285088-krim73zt 65 75 positive positive JJ cord-285088-krim73zt 65 76 rate rate NN cord-285088-krim73zt 65 77 caused cause VBN cord-285088-krim73zt 65 78 by by IN cord-285088-krim73zt 65 79 aerosol aerosol NN cord-285088-krim73zt 65 80 of of IN cord-285088-krim73zt 65 81 the the DT cord-285088-krim73zt 65 82 real real JJ cord-285088-krim73zt 65 83 - - HYPH cord-285088-krim73zt 65 84 time time NN cord-285088-krim73zt 65 85 RT RT NNP cord-285088-krim73zt 65 86 - - HYPH cord-285088-krim73zt 65 87 LAMP LAMP NNP cord-285088-krim73zt 65 88 assay assay NN cord-285088-krim73zt 65 89 was be VBD cord-285088-krim73zt 65 90 higher high JJR cord-285088-krim73zt 65 91 than than IN cord-285088-krim73zt 65 92 that that DT cord-285088-krim73zt 65 93 of of IN cord-285088-krim73zt 65 94 the the DT cord-285088-krim73zt 65 95 visual visual JJ cord-285088-krim73zt 65 96 RT RT NNP cord-285088-krim73zt 65 97 - - HYPH cord-285088-krim73zt 65 98 LAMP LAMP NNP cord-285088-krim73zt 65 99 assay assay NN cord-285088-krim73zt 65 100 which which WDT cord-285088-krim73zt 65 101 sunk sink VBD cord-285088-krim73zt 65 102 the the DT cord-285088-krim73zt 65 103 reaction reaction NN cord-285088-krim73zt 65 104 tube tube NN cord-285088-krim73zt 65 105 in in IN cord-285088-krim73zt 65 106 water water NN cord-285088-krim73zt 65 107 . . . cord-285088-krim73zt 66 1 This this DT cord-285088-krim73zt 66 2 study study NN cord-285088-krim73zt 66 3 had have VBD cord-285088-krim73zt 66 4 established establish VBN cord-285088-krim73zt 66 5 the the DT cord-285088-krim73zt 66 6 one one CD cord-285088-krim73zt 66 7 - - HYPH cord-285088-krim73zt 66 8 pot pot NN cord-285088-krim73zt 66 9 real real JJ cord-285088-krim73zt 66 10 - - HYPH cord-285088-krim73zt 66 11 time time NN cord-285088-krim73zt 66 12 RT RT NNP cord-285088-krim73zt 66 13 - - HYPH cord-285088-krim73zt 66 14 LAMP LAMP NNP cord-285088-krim73zt 66 15 assay assay NN cord-285088-krim73zt 66 16 and and CC cord-285088-krim73zt 66 17 one one CD cord-285088-krim73zt 66 18 - - HYPH cord-285088-krim73zt 66 19 pot pot NN cord-285088-krim73zt 66 20 visual visual JJ cord-285088-krim73zt 66 21 RT rt NN cord-285088-krim73zt 66 22 - - HYPH cord-285088-krim73zt 66 23 LAMP LAMP NNP cord-285088-krim73zt 66 24 assay assay NN cord-285088-krim73zt 66 25 for for IN cord-285088-krim73zt 66 26 the the DT cord-285088-krim73zt 66 27 detection detection NN cord-285088-krim73zt 66 28 of of IN cord-285088-krim73zt 66 29 COVID-19 COVID-19 NNP cord-285088-krim73zt 66 30 , , , cord-285088-krim73zt 66 31 and and CC cord-285088-krim73zt 66 32 provided provide VBD cord-285088-krim73zt 66 33 the the DT cord-285088-krim73zt 66 34 rapid rapid JJ cord-285088-krim73zt 66 35 , , , cord-285088-krim73zt 66 36 reliable reliable JJ cord-285088-krim73zt 66 37 and and CC cord-285088-krim73zt 66 38 sensitive sensitive JJ cord-285088-krim73zt 66 39 tools tool NNS cord-285088-krim73zt 66 40 for for IN cord-285088-krim73zt 66 41 facilitating facilitate VBG cord-285088-krim73zt 66 42 preliminary preliminary JJ cord-285088-krim73zt 66 43 and and CC cord-285088-krim73zt 66 44 cost cost NN cord-285088-krim73zt 66 45 - - HYPH cord-285088-krim73zt 66 46 effective effective JJ cord-285088-krim73zt 66 47 prevention prevention NN cord-285088-krim73zt 66 48 and and CC cord-285088-krim73zt 66 49 control control NN cord-285088-krim73zt 66 50 of of IN cord-285088-krim73zt 66 51 COVID-19 COVID-19 NNP cord-285088-krim73zt 66 52 . . . cord-285088-krim73zt 67 1 NO no DT cord-285088-krim73zt 67 2 external external JJ cord-285088-krim73zt 67 3 funding funding NN cord-285088-krim73zt 68 1 The the DT cord-285088-krim73zt 68 2 COVID-19 covid-19 JJ cord-285088-krim73zt 68 3 epidemic epidemic NN cord-285088-krim73zt 68 4 Chest Chest NNP cord-285088-krim73zt 68 5 Imaging Imaging NNP cord-285088-krim73zt 68 6 Appearance Appearance NNP cord-285088-krim73zt 68 7 of of IN cord-285088-krim73zt 68 8 COVID-19 COVID-19 NNP cord-285088-krim73zt 68 9 Infection Infection NNP cord-285088-krim73zt 68 10 Detection Detection NNP cord-285088-krim73zt 68 11 of of IN cord-285088-krim73zt 68 12 2019 2019 CD cord-285088-krim73zt 68 13 novel novel JJ cord-285088-krim73zt 68 14 coronavirus coronavirus NN cord-285088-krim73zt 68 15 ( ( -LRB- cord-285088-krim73zt 68 16 2019-nCoV 2019-ncov CD cord-285088-krim73zt 68 17 ) ) -RRB- cord-285088-krim73zt 68 18 by by IN cord-285088-krim73zt 68 19 real real JJ cord-285088-krim73zt 68 20 - - HYPH cord-285088-krim73zt 68 21 time time NN cord-285088-krim73zt 68 22 RT RT NNP cord-285088-krim73zt 68 23 - - HYPH cord-285088-krim73zt 68 24 PCR PCR NNP cord-285088-krim73zt 68 25 Loop Loop NNP cord-285088-krim73zt 68 26 - - HYPH cord-285088-krim73zt 68 27 mediated mediate VBN cord-285088-krim73zt 68 28 isothermal isothermal JJ cord-285088-krim73zt 68 29 amplification amplification NN cord-285088-krim73zt 68 30 of of IN cord-285088-krim73zt 68 31 DNA DNA NNP cord-285088-krim73zt 68 32 Accelerated accelerate VBN cord-285088-krim73zt 68 33 reaction reaction NN cord-285088-krim73zt 68 34 by by IN cord-285088-krim73zt 68 35 loop loop NN cord-285088-krim73zt 68 36 - - HYPH cord-285088-krim73zt 68 37 mediated mediate VBN cord-285088-krim73zt 68 38 isothermal isothermal JJ cord-285088-krim73zt 68 39 amplification amplification NN cord-285088-krim73zt 68 40 using use VBG cord-285088-krim73zt 68 41 loop loop NN cord-285088-krim73zt 68 42 primers primer NNS cord-285088-krim73zt 68 43 Development development NN cord-285088-krim73zt 68 44 and and CC cord-285088-krim73zt 68 45 evaluation evaluation NN cord-285088-krim73zt 68 46 of of IN cord-285088-krim73zt 68 47 a a DT cord-285088-krim73zt 68 48 loop loop NN cord-285088-krim73zt 68 49 - - HYPH cord-285088-krim73zt 68 50 mediated mediate VBN cord-285088-krim73zt 68 51 isothermal isothermal JJ cord-285088-krim73zt 68 52 amplification amplification NN cord-285088-krim73zt 68 53 ( ( -LRB- cord-285088-krim73zt 68 54 LAMP lamp NN cord-285088-krim73zt 68 55 ) ) -RRB- cord-285088-krim73zt 68 56 method method NN cord-285088-krim73zt 68 57 for for IN cord-285088-krim73zt 68 58 detecting detect VBG cord-285088-krim73zt 68 59 Listeria Listeria NNP cord-285088-krim73zt 68 60 monocytogenes monocytogene NNS cord-285088-krim73zt 68 61 in in IN cord-285088-krim73zt 68 62 raw raw JJ cord-285088-krim73zt 68 63 milk milk NN cord-285088-krim73zt 68 64 Rapid Rapid NNP cord-285088-krim73zt 68 65 Detection Detection NNP cord-285088-krim73zt 68 66 of of IN cord-285088-krim73zt 68 67 Listeria Listeria NNP cord-285088-krim73zt 68 68 monocytogenes monocytogene NNS cord-285088-krim73zt 68 69 in in IN cord-285088-krim73zt 68 70 raw raw JJ cord-285088-krim73zt 68 71 milk milk NN cord-285088-krim73zt 68 72 with with IN cord-285088-krim73zt 68 73 loop loop NN cord-285088-krim73zt 68 74 - - HYPH cord-285088-krim73zt 68 75 mediated mediate VBN cord-285088-krim73zt 68 76 isothermal isothermal JJ cord-285088-krim73zt 68 77 amplification amplification NN cord-285088-krim73zt 68 78 and and CC cord-285088-krim73zt 68 79 chemosensor chemosensor NNP cord-285088-krim73zt 68 80 Evaluation Evaluation NNP cord-285088-krim73zt 68 81 and and CC cord-285088-krim73zt 68 82 improvement improvement NN cord-285088-krim73zt 68 83 of of IN cord-285088-krim73zt 68 84 LAMP lamp NN cord-285088-krim73zt 68 85 assays assay NNS cord-285088-krim73zt 68 86 for for IN cord-285088-krim73zt 68 87 detection detection NN cord-285088-krim73zt 68 88 of of IN cord-285088-krim73zt 68 89 Escherichia Escherichia NNP cord-285088-krim73zt 68 90 coli coli VBZ cord-285088-krim73zt 68 91 serogroups serogroup NNS cord-285088-krim73zt 69 1 O26 O26 NNP cord-285088-krim73zt 69 2 , , , cord-285088-krim73zt 69 3 O45 O45 NNP cord-285088-krim73zt 69 4 , , , cord-285088-krim73zt 69 5 O103 O103 NNP cord-285088-krim73zt 69 6 , , , cord-285088-krim73zt 69 7 O111 O111 NNP cord-285088-krim73zt 69 8 , , , cord-285088-krim73zt 69 9 O121 O121 NNP cord-285088-krim73zt 69 10 , , , cord-285088-krim73zt 69 11 O145 O145 NNP cord-285088-krim73zt 69 12 , , , cord-285088-krim73zt 69 13 and and CC cord-285088-krim73zt 69 14 O157 O157 NNP cord-285088-krim73zt 69 15 . . . cord-285088-krim73zt 70 1 Afri Afri NNP cord-285088-krim73zt 70 2 Health Health NNP cord-285088-krim73zt 70 3 Sci Sci NNP cord-285088-krim73zt 70 4 Development Development NNP cord-285088-krim73zt 70 5 of of IN cord-285088-krim73zt 70 6 a a DT cord-285088-krim73zt 70 7 real real JJ cord-285088-krim73zt 70 8 - - HYPH cord-285088-krim73zt 70 9 time time NN cord-285088-krim73zt 70 10 loop loop NN cord-285088-krim73zt 70 11 - - HYPH cord-285088-krim73zt 70 12 mediated mediate VBN cord-285088-krim73zt 70 13 isothermal isothermal JJ cord-285088-krim73zt 70 14 amplification amplification NN cord-285088-krim73zt 70 15 ( ( -LRB- cord-285088-krim73zt 70 16 LAMP lamp NN cord-285088-krim73zt 70 17 ) ) -RRB- cord-285088-krim73zt 70 18 assay assay NN cord-285088-krim73zt 70 19 and and CC cord-285088-krim73zt 70 20 visual visual JJ cord-285088-krim73zt 70 21 LAMP LAMP NNP cord-285088-krim73zt 70 22 assay assay NN cord-285088-krim73zt 70 23 for for IN cord-285088-krim73zt 70 24 detection detection NN cord-285088-krim73zt 70 25 of of IN cord-285088-krim73zt 70 26 African african JJ cord-285088-krim73zt 70 27 swine swine NN cord-285088-krim73zt 70 28 fever fever NN cord-285088-krim73zt 70 29 virus virus NN cord-285088-krim73zt 70 30 ( ( -LRB- cord-285088-krim73zt 70 31 ASFV ASFV NNP cord-285088-krim73zt 70 32 ) ) -RRB- cord-285088-krim73zt 70 33 Effect Effect NNP cord-285088-krim73zt 70 34 of of IN cord-285088-krim73zt 70 35 internal internal JJ cord-285088-krim73zt 70 36 primer primer NN cord-285088-krim73zt 70 37 - - HYPH cord-285088-krim73zt 70 38 template template NN cord-285088-krim73zt 70 39 mismatches mismatch NNS cord-285088-krim73zt 70 40 on on IN cord-285088-krim73zt 70 41 loop loop NN cord-285088-krim73zt 70 42 - - HYPH cord-285088-krim73zt 70 43 mediated mediate VBN cord-285088-krim73zt 70 44 isothermal isothermal JJ cord-285088-krim73zt 70 45 amplification amplification NN cord-285088-krim73zt 71 1 A a DT cord-285088-krim73zt 71 2 novel novel JJ cord-285088-krim73zt 71 3 thermostable thermostable JJ cord-285088-krim73zt 71 4 polymerase polymerase NN cord-285088-krim73zt 71 5 for for IN cord-285088-krim73zt 71 6 RNA RNA NNP cord-285088-krim73zt 71 7 and and CC cord-285088-krim73zt 71 8 DNA DNA NNP cord-285088-krim73zt 71 9 Loop Loop NNP cord-285088-krim73zt 71 10 - - HYPH cord-285088-krim73zt 71 11 mediated mediate VBN cord-285088-krim73zt 71 12 isothermal isothermal JJ cord-285088-krim73zt 71 13 amplification amplification NN cord-285088-krim73zt 71 14 ( ( -LRB- cord-285088-krim73zt 71 15 LAMP lamp NN cord-285088-krim73zt 71 16 ) ) -RRB- cord-285088-krim73zt 72 1 Innate Innate NNP cord-285088-krim73zt 72 2 Reverse Reverse NNP cord-285088-krim73zt 72 3 Transcriptase Transcriptase NNP cord-285088-krim73zt 72 4 Activity activity NN cord-285088-krim73zt 72 5 of of IN cord-285088-krim73zt 72 6 DNA dna NN cord-285088-krim73zt 72 7 Polymerase Polymerase NNP cord-285088-krim73zt 72 8 for for IN cord-285088-krim73zt 72 9 Isothermal Isothermal NNP cord-285088-krim73zt 72 10 RNA RNA NNP cord-285088-krim73zt 72 11 Direct Direct NNP cord-285088-krim73zt 72 12 Detection detection NN cord-285088-krim73zt 73 1 The the DT cord-285088-krim73zt 73 2 author author NN cord-285088-krim73zt 73 3 declares declare VBZ cord-285088-krim73zt 73 4 no no DT cord-285088-krim73zt 73 5 competing compete VBG cord-285088-krim73zt 73 6 interests interest NNS cord-285088-krim73zt 73 7 . . .