id	sid	tid	token	lemma	pos
cord-324094-23kzr8rq	1	1	key	key	NN
cord-324094-23kzr8rq	1	2	:	:	:
cord-324094-23kzr8rq	2	1	cord-324094	cord-324094	NN
cord-324094-23kzr8rq	2	2	-	-	HYPH
cord-324094-23kzr8rq	2	3	23kzr8rq	23kzr8rq	JJ
cord-324094-23kzr8rq	2	4	authors	author	NNS
cord-324094-23kzr8rq	2	5	:	:	:
cord-324094-23kzr8rq	2	6	Parida	Parida	NNP
cord-324094-23kzr8rq	2	7	,	,	,
cord-324094-23kzr8rq	2	8	M.	M.	NNP
cord-324094-23kzr8rq	2	9	M.	M.	NNP
cord-324094-23kzr8rq	2	10	title	title	NN
cord-324094-23kzr8rq	2	11	:	:	:
cord-324094-23kzr8rq	2	12	Rapid	rapid	JJ
cord-324094-23kzr8rq	2	13	and	and	CC
cord-324094-23kzr8rq	2	14	real	real	JJ
cord-324094-23kzr8rq	2	15	-	-	HYPH
cord-324094-23kzr8rq	2	16	time	time	NN
cord-324094-23kzr8rq	2	17	detection	detection	NN
cord-324094-23kzr8rq	2	18	technologies	technology	NNS
cord-324094-23kzr8rq	2	19	for	for	IN
cord-324094-23kzr8rq	2	20	emerging	emerge	VBG
cord-324094-23kzr8rq	2	21	viruses	virus	NNS
cord-324094-23kzr8rq	2	22	of	of	IN
cord-324094-23kzr8rq	2	23	biomedical	biomedical	JJ
cord-324094-23kzr8rq	2	24	importance	importance	NN
cord-324094-23kzr8rq	2	25	date	date	NN
cord-324094-23kzr8rq	2	26	:	:	:
cord-324094-23kzr8rq	2	27	2008	2008	CD
cord-324094-23kzr8rq	2	28	-	-	SYM
cord-324094-23kzr8rq	2	29	11	11	CD
cord-324094-23kzr8rq	2	30	-	-	HYPH
cord-324094-23kzr8rq	2	31	01	01	CD
cord-324094-23kzr8rq	2	32	journal	journal	NN
cord-324094-23kzr8rq	2	33	:	:	:
cord-324094-23kzr8rq	2	34	J	J	NNP
cord-324094-23kzr8rq	2	35	Biosci	Biosci	NNP
cord-324094-23kzr8rq	2	36	DOI	DOI	NNP
cord-324094-23kzr8rq	2	37	:	:	:
cord-324094-23kzr8rq	2	38	10.1007	10.1007	CD
cord-324094-23kzr8rq	2	39	/	/	SYM
cord-324094-23kzr8rq	2	40	s12038	s12038	NNP
cord-324094-23kzr8rq	2	41	-	-	HYPH
cord-324094-23kzr8rq	2	42	008	008	CD
cord-324094-23kzr8rq	2	43	-	-	HYPH
cord-324094-23kzr8rq	2	44	0079	0079	CD
cord-324094-23kzr8rq	2	45	-	-	SYM
cord-324094-23kzr8rq	2	46	7	7	CD
cord-324094-23kzr8rq	2	47	sha	sha	NNP
cord-324094-23kzr8rq	2	48	:	:	:
cord-324094-23kzr8rq	3	1	08a5c34ec22e96c9763e99e5b5a6f4df109ed0d8	08a5c34ec22e96c9763e99e5b5a6f4df109ed0d8	CD
cord-324094-23kzr8rq	4	1	doc_id	doc_id	CD
cord-324094-23kzr8rq	5	1	:	:	:
cord-324094-23kzr8rq	5	2	324094	324094	CD
cord-324094-23kzr8rq	5	3	cord_uid	cord_uid	NNS
cord-324094-23kzr8rq	5	4	:	:	:
cord-324094-23kzr8rq	6	1	23kzr8rq	23kzr8rq	LS
cord-324094-23kzr8rq	7	1	The	the	DT
cord-324094-23kzr8rq	7	2	development	development	NN
cord-324094-23kzr8rq	7	3	of	of	IN
cord-324094-23kzr8rq	7	4	technologies	technology	NNS
cord-324094-23kzr8rq	7	5	with	with	IN
cord-324094-23kzr8rq	7	6	rapid	rapid	JJ
cord-324094-23kzr8rq	7	7	and	and	CC
cord-324094-23kzr8rq	7	8	sensitive	sensitive	JJ
cord-324094-23kzr8rq	7	9	detection	detection	NN
cord-324094-23kzr8rq	7	10	capabilities	capability	NNS
cord-324094-23kzr8rq	7	11	and	and	CC
cord-324094-23kzr8rq	7	12	increased	increase	VBN
cord-324094-23kzr8rq	7	13	throughput	throughput	NN
cord-324094-23kzr8rq	7	14	have	have	VBP
cord-324094-23kzr8rq	7	15	become	become	VBN
cord-324094-23kzr8rq	7	16	crucial	crucial	JJ
cord-324094-23kzr8rq	7	17	for	for	IN
cord-324094-23kzr8rq	7	18	responding	respond	VBG
cord-324094-23kzr8rq	7	19	to	to	IN
cord-324094-23kzr8rq	7	20	greater	great	JJR
cord-324094-23kzr8rq	7	21	number	number	NN
cord-324094-23kzr8rq	7	22	threats	threat	NNS
cord-324094-23kzr8rq	7	23	posed	pose	VBN
cord-324094-23kzr8rq	7	24	by	by	IN
cord-324094-23kzr8rq	7	25	emerging	emerge	VBG
cord-324094-23kzr8rq	7	26	and	and	CC
cord-324094-23kzr8rq	7	27	re	re	VB
cord-324094-23kzr8rq	7	28	-	-	JJ
cord-324094-23kzr8rq	7	29	emerging	emerge	VBG
cord-324094-23kzr8rq	7	30	viruses	virus	NNS
cord-324094-23kzr8rq	7	31	in	in	IN
cord-324094-23kzr8rq	7	32	the	the	DT
cord-324094-23kzr8rq	7	33	recent	recent	JJ
cord-324094-23kzr8rq	7	34	past	past	NN
cord-324094-23kzr8rq	7	35	.	.	.
cord-324094-23kzr8rq	8	1	The	the	DT
cord-324094-23kzr8rq	8	2	conventional	conventional	JJ
cord-324094-23kzr8rq	8	3	identification	identification	NN
cord-324094-23kzr8rq	8	4	methods	method	NNS
cord-324094-23kzr8rq	8	5	require	require	VBP
cord-324094-23kzr8rq	8	6	time	time	NN
cord-324094-23kzr8rq	8	7	-	-	HYPH
cord-324094-23kzr8rq	8	8	consuming	consume	VBG
cord-324094-23kzr8rq	8	9	culturing	culturing	NN
cord-324094-23kzr8rq	8	10	,	,	,
cord-324094-23kzr8rq	8	11	and/	and/	CD
cord-324094-23kzr8rq	8	12	or	or	CC
cord-324094-23kzr8rq	8	13	detection	detection	NN
cord-324094-23kzr8rq	8	14	of	of	IN
cord-324094-23kzr8rq	8	15	antibodies	antibody	NNS
cord-324094-23kzr8rq	8	16	,	,	,
cord-324094-23kzr8rq	8	17	which	which	WDT
cord-324094-23kzr8rq	8	18	are	be	VBP
cord-324094-23kzr8rq	8	19	not	not	RB
cord-324094-23kzr8rq	8	20	very	very	RB
cord-324094-23kzr8rq	8	21	sensitive	sensitive	JJ
cord-324094-23kzr8rq	8	22	and	and	CC
cord-324094-23kzr8rq	8	23	specific	specific	JJ
cord-324094-23kzr8rq	8	24	.	.	.
cord-324094-23kzr8rq	9	1	The	the	DT
cord-324094-23kzr8rq	9	2	recent	recent	JJ
cord-324094-23kzr8rq	9	3	advances	advance	NNS
cord-324094-23kzr8rq	9	4	in	in	IN
cord-324094-23kzr8rq	9	5	molecular	molecular	JJ
cord-324094-23kzr8rq	9	6	biology	biology	NN
cord-324094-23kzr8rq	9	7	techniques	technique	NNS
cord-324094-23kzr8rq	9	8	in	in	IN
cord-324094-23kzr8rq	9	9	the	the	DT
cord-324094-23kzr8rq	9	10	field	field	NN
cord-324094-23kzr8rq	9	11	of	of	IN
cord-324094-23kzr8rq	9	12	genomics	genomic	NNS
cord-324094-23kzr8rq	9	13	and	and	CC
cord-324094-23kzr8rq	9	14	proteomics	proteomic	NNS
cord-324094-23kzr8rq	9	15	greatly	greatly	RB
cord-324094-23kzr8rq	9	16	facilitate	facilitate	VBP
cord-324094-23kzr8rq	9	17	the	the	DT
cord-324094-23kzr8rq	9	18	rapid	rapid	JJ
cord-324094-23kzr8rq	9	19	identification	identification	NN
cord-324094-23kzr8rq	9	20	with	with	IN
cord-324094-23kzr8rq	9	21	more	more	JJR
cord-324094-23kzr8rq	9	22	accuracy	accuracy	NN
cord-324094-23kzr8rq	9	23	.	.	.
cord-324094-23kzr8rq	10	1	We	-PRON-	PRP
cord-324094-23kzr8rq	10	2	have	have	VBP
cord-324094-23kzr8rq	10	3	developed	develop	VBN
cord-324094-23kzr8rq	10	4	two	two	CD
cord-324094-23kzr8rq	10	5	real	real	JJ
cord-324094-23kzr8rq	10	6	-	-	HYPH
cord-324094-23kzr8rq	10	7	time	time	NN
cord-324094-23kzr8rq	10	8	assays	assay	NNS
cord-324094-23kzr8rq	10	9	ie	ie	FW
cord-324094-23kzr8rq	10	10	.	.	.
cord-324094-23kzr8rq	11	1	,	,	,
cord-324094-23kzr8rq	11	2	SYBR	SYBR	NNP
cord-324094-23kzr8rq	11	3	green	green	JJ
cord-324094-23kzr8rq	12	1	I	-PRON-	PRP
cord-324094-23kzr8rq	12	2	based	base	VBD
cord-324094-23kzr8rq	12	3	real	real	JJ
cord-324094-23kzr8rq	12	4	time	time	NN
cord-324094-23kzr8rq	12	5	reverse	reverse	NN
cord-324094-23kzr8rq	12	6	transcription	transcription	NN
cord-324094-23kzr8rq	12	7	polymerase	polymerase	NN
cord-324094-23kzr8rq	12	8	chain	chain	NN
cord-324094-23kzr8rq	12	9	reaction	reaction	NN
cord-324094-23kzr8rq	12	10	(	(	-LRB-
cord-324094-23kzr8rq	12	11	RT	RT	NNP
cord-324094-23kzr8rq	12	12	-	-	HYPH
cord-324094-23kzr8rq	12	13	PCR	PCR	NNP
cord-324094-23kzr8rq	12	14	)	)	-RRB-
cord-324094-23kzr8rq	12	15	and	and	CC
cord-324094-23kzr8rq	12	16	RT	rt	NN
cord-324094-23kzr8rq	12	17	-	-	HYPH
cord-324094-23kzr8rq	12	18	loop	loop	NN
cord-324094-23kzr8rq	12	19	-	-	HYPH
cord-324094-23kzr8rq	12	20	mediated	mediate	VBN
cord-324094-23kzr8rq	12	21	isothermal	isothermal	JJ
cord-324094-23kzr8rq	12	22	amplification	amplification	NN
cord-324094-23kzr8rq	12	23	(	(	-LRB-
cord-324094-23kzr8rq	12	24	LAMP	lamp	NN
cord-324094-23kzr8rq	12	25	)	)	-RRB-
cord-324094-23kzr8rq	12	26	assay	assay	NN
cord-324094-23kzr8rq	12	27	for	for	IN
cord-324094-23kzr8rq	12	28	rapid	rapid	JJ
cord-324094-23kzr8rq	12	29	detection	detection	NN
cord-324094-23kzr8rq	12	30	as	as	RB
cord-324094-23kzr8rq	12	31	well	well	RB
cord-324094-23kzr8rq	12	32	as	as	IN
cord-324094-23kzr8rq	12	33	typing	typing	NN
cord-324094-23kzr8rq	12	34	of	of	IN
cord-324094-23kzr8rq	12	35	some	some	DT
cord-324094-23kzr8rq	12	36	of	of	IN
cord-324094-23kzr8rq	12	37	the	the	DT
cord-324094-23kzr8rq	12	38	emerging	emerge	VBG
cord-324094-23kzr8rq	12	39	viruses	virus	NNS
cord-324094-23kzr8rq	12	40	of	of	IN
cord-324094-23kzr8rq	12	41	biomedical	biomedical	JJ
cord-324094-23kzr8rq	12	42	importance	importance	NN
cord-324094-23kzr8rq	12	43	viz	viz	NN
cord-324094-23kzr8rq	12	44	.	.	.
cord-324094-23kzr8rq	13	1	dengue	dengue	NN
cord-324094-23kzr8rq	13	2	,	,	,
cord-324094-23kzr8rq	13	3	Japanese	japanese	JJ
cord-324094-23kzr8rq	13	4	encephalitis	encephalitis	RB
cord-324094-23kzr8rq	13	5	,	,	,
cord-324094-23kzr8rq	13	6	chikungunya	chikungunya	NN
cord-324094-23kzr8rq	13	7	,	,	,
cord-324094-23kzr8rq	13	8	west	west	NNP
cord-324094-23kzr8rq	13	9	Nile	Nile	NNP
cord-324094-23kzr8rq	13	10	,	,	,
cord-324094-23kzr8rq	13	11	severe	severe	JJ
cord-324094-23kzr8rq	13	12	acute	acute	JJ
cord-324094-23kzr8rq	13	13	respiratory	respiratory	JJ
cord-324094-23kzr8rq	13	14	syndrome	syndrome	NN
cord-324094-23kzr8rq	13	15	virus	virus	NN
cord-324094-23kzr8rq	13	16	(	(	-LRB-
cord-324094-23kzr8rq	13	17	SARS	SARS	NNP
cord-324094-23kzr8rq	13	18	)	)	-RRB-
cord-324094-23kzr8rq	13	19	etc	etc	FW
cord-324094-23kzr8rq	13	20	.	.	.
cord-324094-23kzr8rq	14	1	Both	both	CC
cord-324094-23kzr8rq	14	2	these	these	DT
cord-324094-23kzr8rq	14	3	techniques	technique	NNS
cord-324094-23kzr8rq	14	4	are	be	VBP
cord-324094-23kzr8rq	14	5	capable	capable	JJ
cord-324094-23kzr8rq	14	6	of	of	IN
cord-324094-23kzr8rq	14	7	detection	detection	NN
cord-324094-23kzr8rq	14	8	and	and	CC
cord-324094-23kzr8rq	14	9	differentiation	differentiation	NN
cord-324094-23kzr8rq	14	10	as	as	RB
cord-324094-23kzr8rq	14	11	well	well	RB
cord-324094-23kzr8rq	14	12	as	as	IN
cord-324094-23kzr8rq	14	13	quantifying	quantify	VBG
cord-324094-23kzr8rq	14	14	viral	viral	JJ
cord-324094-23kzr8rq	14	15	load	load	NN
cord-324094-23kzr8rq	14	16	with	with	IN
cord-324094-23kzr8rq	14	17	higher	high	JJR
cord-324094-23kzr8rq	14	18	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	14	19	,	,	,
cord-324094-23kzr8rq	14	20	rapidity	rapidity	NN
cord-324094-23kzr8rq	14	21	,	,	,
cord-324094-23kzr8rq	14	22	specificity	specificity	NN
cord-324094-23kzr8rq	14	23	.	.	.
cord-324094-23kzr8rq	15	1	One	one	CD
cord-324094-23kzr8rq	15	2	of	of	IN
cord-324094-23kzr8rq	15	3	the	the	DT
cord-324094-23kzr8rq	15	4	most	most	RBS
cord-324094-23kzr8rq	15	5	important	important	JJ
cord-324094-23kzr8rq	15	6	advantages	advantage	NNS
cord-324094-23kzr8rq	15	7	of	of	IN
cord-324094-23kzr8rq	15	8	LAMP	lamp	NN
cord-324094-23kzr8rq	15	9	is	be	VBZ
cord-324094-23kzr8rq	15	10	its	-PRON-	PRP$
cord-324094-23kzr8rq	15	11	field	field	NN
cord-324094-23kzr8rq	15	12	applicability	applicability	NN
cord-324094-23kzr8rq	15	13	,	,	,
cord-324094-23kzr8rq	15	14	without	without	IN
cord-324094-23kzr8rq	15	15	requirement	requirement	NN
cord-324094-23kzr8rq	15	16	of	of	IN
cord-324094-23kzr8rq	15	17	any	any	DT
cord-324094-23kzr8rq	15	18	sophisticated	sophisticated	JJ
cord-324094-23kzr8rq	15	19	equipments	equipment	NNS
cord-324094-23kzr8rq	15	20	.	.	.
cord-324094-23kzr8rq	16	1	Both	both	CC
cord-324094-23kzr8rq	16	2	these	these	DT
cord-324094-23kzr8rq	16	3	assays	assay	NNS
cord-324094-23kzr8rq	16	4	have	have	VBP
cord-324094-23kzr8rq	16	5	been	be	VBN
cord-324094-23kzr8rq	16	6	extensively	extensively	RB
cord-324094-23kzr8rq	16	7	evaluated	evaluate	VBN
cord-324094-23kzr8rq	16	8	and	and	CC
cord-324094-23kzr8rq	16	9	validated	validate	VBN
cord-324094-23kzr8rq	16	10	with	with	IN
cord-324094-23kzr8rq	16	11	clinical	clinical	JJ
cord-324094-23kzr8rq	16	12	samples	sample	NNS
cord-324094-23kzr8rq	16	13	of	of	IN
cord-324094-23kzr8rq	16	14	recent	recent	JJ
cord-324094-23kzr8rq	16	15	epidemics	epidemic	NNS
cord-324094-23kzr8rq	16	16	from	from	IN
cord-324094-23kzr8rq	16	17	different	different	JJ
cord-324094-23kzr8rq	16	18	parts	part	NNS
cord-324094-23kzr8rq	16	19	of	of	IN
cord-324094-23kzr8rq	16	20	India	India	NNP
cord-324094-23kzr8rq	16	21	.	.	.
cord-324094-23kzr8rq	17	1	The	the	DT
cord-324094-23kzr8rq	17	2	establishment	establishment	NN
cord-324094-23kzr8rq	17	3	of	of	IN
cord-324094-23kzr8rq	17	4	these	these	DT
cord-324094-23kzr8rq	17	5	real	real	JJ
cord-324094-23kzr8rq	17	6	time	time	NN
cord-324094-23kzr8rq	17	7	molecular	molecular	JJ
cord-324094-23kzr8rq	17	8	assays	assay	NNS
cord-324094-23kzr8rq	17	9	will	will	MD
cord-324094-23kzr8rq	17	10	certainly	certainly	RB
cord-324094-23kzr8rq	17	11	facilitate	facilitate	VB
cord-324094-23kzr8rq	17	12	the	the	DT
cord-324094-23kzr8rq	17	13	rapid	rapid	JJ
cord-324094-23kzr8rq	17	14	detection	detection	NN
cord-324094-23kzr8rq	17	15	of	of	IN
cord-324094-23kzr8rq	17	16	viruses	virus	NNS
cord-324094-23kzr8rq	17	17	with	with	IN
cord-324094-23kzr8rq	17	18	high	high	JJ
cord-324094-23kzr8rq	17	19	degree	degree	NN
cord-324094-23kzr8rq	17	20	of	of	IN
cord-324094-23kzr8rq	17	21	precision	precision	NN
cord-324094-23kzr8rq	17	22	and	and	CC
cord-324094-23kzr8rq	17	23	accuracy	accuracy	NN
cord-324094-23kzr8rq	17	24	in	in	IN
cord-324094-23kzr8rq	17	25	future	future	NN
cord-324094-23kzr8rq	17	26	.	.	.
cord-324094-23kzr8rq	18	1	The	the	DT
cord-324094-23kzr8rq	18	2	rapid	rapid	JJ
cord-324094-23kzr8rq	18	3	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	18	4	of	of	IN
cord-324094-23kzr8rq	18	5	virus	virus	NN
cord-324094-23kzr8rq	18	6	diseases	disease	NNS
cord-324094-23kzr8rq	18	7	has	have	VBZ
cord-324094-23kzr8rq	18	8	assumed	assume	VBN
cord-324094-23kzr8rq	18	9	greater	great	JJR
cord-324094-23kzr8rq	18	10	signifi	signifi	NNP
cord-324094-23kzr8rq	18	11	cance	cance	NN
cord-324094-23kzr8rq	18	12	owing	owe	VBG
cord-324094-23kzr8rq	18	13	to	to	IN
cord-324094-23kzr8rq	18	14	the	the	DT
cord-324094-23kzr8rq	18	15	direct	direct	JJ
cord-324094-23kzr8rq	18	16	benefi	benefi	NNP
cord-324094-23kzr8rq	18	17	t	t	NN
cord-324094-23kzr8rq	18	18	of	of	IN
cord-324094-23kzr8rq	18	19	patient	patient	JJ
cord-324094-23kzr8rq	18	20	management	management	NN
cord-324094-23kzr8rq	18	21	in	in	IN
cord-324094-23kzr8rq	18	22	absence	absence	NN
cord-324094-23kzr8rq	18	23	of	of	IN
cord-324094-23kzr8rq	18	24	suitable	suitable	JJ
cord-324094-23kzr8rq	18	25	therapeutic	therapeutic	JJ
cord-324094-23kzr8rq	18	26	and	and	CC
cord-324094-23kzr8rq	18	27	prophylactic	prophylactic	JJ
cord-324094-23kzr8rq	18	28	measures	measure	NNS
cord-324094-23kzr8rq	18	29	.	.	.
cord-324094-23kzr8rq	19	1	With	with	IN
cord-324094-23kzr8rq	19	2	the	the	DT
cord-324094-23kzr8rq	19	3	advancement	advancement	NN
cord-324094-23kzr8rq	19	4	of	of	IN
cord-324094-23kzr8rq	19	5	technologies	technology	NNS
cord-324094-23kzr8rq	19	6	,	,	,
cord-324094-23kzr8rq	19	7	increasingly	increasingly	RB
cord-324094-23kzr8rq	19	8	diverse	diverse	JJ
cord-324094-23kzr8rq	19	9	methods	method	NNS
cord-324094-23kzr8rq	19	10	are	be	VBP
cord-324094-23kzr8rq	19	11	available	available	JJ
cord-324094-23kzr8rq	19	12	which	which	WDT
cord-324094-23kzr8rq	19	13	make	make	VBP
cord-324094-23kzr8rq	19	14	it	-PRON-	PRP
cord-324094-23kzr8rq	19	15	possible	possible	JJ
cord-324094-23kzr8rq	19	16	to	to	TO
cord-324094-23kzr8rq	19	17	detect	detect	VB
cord-324094-23kzr8rq	19	18	and	and	CC
cord-324094-23kzr8rq	19	19	analyze	analyze	VB
cord-324094-23kzr8rq	19	20	any	any	DT
cord-324094-23kzr8rq	19	21	virus	virus	NN
cord-324094-23kzr8rq	19	22	,	,	,
cord-324094-23kzr8rq	19	23	including	include	VBG
cord-324094-23kzr8rq	19	24	those	those	DT
cord-324094-23kzr8rq	19	25	,	,	,
cord-324094-23kzr8rq	19	26	which	which	WDT
cord-324094-23kzr8rq	19	27	can	can	MD
cord-324094-23kzr8rq	19	28	not	not	RB
cord-324094-23kzr8rq	19	29	be	be	VB
cord-324094-23kzr8rq	19	30	cultured	cultured	JJ
cord-324094-23kzr8rq	19	31	.	.	.
cord-324094-23kzr8rq	20	1	The	the	DT
cord-324094-23kzr8rq	20	2	laboratory	laboratory	NN
cord-324094-23kzr8rq	20	3	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	20	4	of	of	IN
cord-324094-23kzr8rq	20	5	virus	virus	NN
cord-324094-23kzr8rq	20	6	infection	infection	NN
cord-324094-23kzr8rq	20	7	can	can	MD
cord-324094-23kzr8rq	20	8	be	be	VB
cord-324094-23kzr8rq	20	9	made	make	VBN
cord-324094-23kzr8rq	20	10	by	by	IN
cord-324094-23kzr8rq	20	11	the	the	DT
cord-324094-23kzr8rq	20	12	detection	detection	NN
cord-324094-23kzr8rq	20	13	of	of	IN
cord-324094-23kzr8rq	20	14	specifi	specifi	NNP
cord-324094-23kzr8rq	20	15	c	c	NNP
cord-324094-23kzr8rq	20	16	virus	virus	NN
cord-324094-23kzr8rq	20	17	,	,	,
cord-324094-23kzr8rq	20	18	viral	viral	JJ
cord-324094-23kzr8rq	20	19	antigen	antigen	NN
cord-324094-23kzr8rq	20	20	,	,	,
cord-324094-23kzr8rq	20	21	genomic	genomic	JJ
cord-324094-23kzr8rq	20	22	sequences	sequence	NNS
cord-324094-23kzr8rq	20	23	and/or	and/or	CC
cord-324094-23kzr8rq	20	24	antibodies	antibody	NNS
cord-324094-23kzr8rq	20	25	.	.	.
cord-324094-23kzr8rq	21	1	Nucleic	nucleic	JJ
cord-324094-23kzr8rq	21	2	acid	acid	NN
cord-324094-23kzr8rq	21	3	amplifi	amplifi	NNP
cord-324094-23kzr8rq	21	4	cation	cation	NN
cord-324094-23kzr8rq	21	5	is	be	VBZ
cord-324094-23kzr8rq	21	6	one	one	CD
cord-324094-23kzr8rq	21	7	of	of	IN
cord-324094-23kzr8rq	21	8	the	the	DT
cord-324094-23kzr8rq	21	9	most	most	RBS
cord-324094-23kzr8rq	21	10	valuable	valuable	JJ
cord-324094-23kzr8rq	21	11	tools	tool	NNS
cord-324094-23kzr8rq	21	12	in	in	IN
cord-324094-23kzr8rq	21	13	virtually	virtually	RB
cord-324094-23kzr8rq	21	14	all	all	DT
cord-324094-23kzr8rq	21	15	life	life	NN
cord-324094-23kzr8rq	21	16	science	science	NN
cord-324094-23kzr8rq	21	17	fi	fi	NN
cord-324094-23kzr8rq	21	18	elds	elds	NNP
cord-324094-23kzr8rq	21	19	,	,	,
cord-324094-23kzr8rq	21	20	including	include	VBG
cord-324094-23kzr8rq	21	21	application	application	NN
cord-324094-23kzr8rq	21	22	-	-	HYPH
cord-324094-23kzr8rq	21	23	oriented	orient	VBN
cord-324094-23kzr8rq	21	24	fi	fi	NN
cord-324094-23kzr8rq	21	25	elds	eld	NNS
cord-324094-23kzr8rq	21	26	such	such	JJ
cord-324094-23kzr8rq	21	27	as	as	IN
cord-324094-23kzr8rq	21	28	clinical	clinical	JJ
cord-324094-23kzr8rq	21	29	medicine	medicine	NN
cord-324094-23kzr8rq	21	30	in	in	IN
cord-324094-23kzr8rq	21	31	which	which	WDT
cord-324094-23kzr8rq	21	32	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	21	33	of	of	IN
cord-324094-23kzr8rq	21	34	infectious	infectious	JJ
cord-324094-23kzr8rq	21	35	diseases	disease	NNS
cord-324094-23kzr8rq	21	36	,	,	,
cord-324094-23kzr8rq	21	37	genetic	genetic	JJ
cord-324094-23kzr8rq	21	38	disorders	disorder	NNS
cord-324094-23kzr8rq	21	39	and	and	CC
cord-324094-23kzr8rq	21	40	genetic	genetic	JJ
cord-324094-23kzr8rq	21	41	traits	trait	NNS
cord-324094-23kzr8rq	21	42	are	be	VBP
cord-324094-23kzr8rq	21	43	particularly	particularly	RB
cord-324094-23kzr8rq	21	44	benefi	benefi	NNP
cord-324094-23kzr8rq	21	45	ted	ted	NNP
cord-324094-23kzr8rq	21	46	by	by	IN
cord-324094-23kzr8rq	21	47	this	this	DT
cord-324094-23kzr8rq	21	48	new	new	JJ
cord-324094-23kzr8rq	21	49	technique	technique	NN
cord-324094-23kzr8rq	21	50	.	.	.
cord-324094-23kzr8rq	22	1	Now	now	RB
cord-324094-23kzr8rq	22	2	a	a	DT
cord-324094-23kzr8rq	22	3	days	day	NNS
cord-324094-23kzr8rq	22	4	,	,	,
cord-324094-23kzr8rq	22	5	several	several	JJ
cord-324094-23kzr8rq	22	6	amplifi	amplifi	NNP
cord-324094-23kzr8rq	22	7	cation	cation	NN
cord-324094-23kzr8rq	22	8	methods	method	NNS
cord-324094-23kzr8rq	22	9	have	have	VBP
cord-324094-23kzr8rq	22	10	been	be	VBN
cord-324094-23kzr8rq	22	11	invented	invent	VBN
cord-324094-23kzr8rq	22	12	viz	viz	NN
cord-324094-23kzr8rq	22	13	.	.	.
cord-324094-23kzr8rq	23	1	nucleic	nucleic	JJ
cord-324094-23kzr8rq	23	2	acid	acid	NN
cord-324094-23kzr8rq	23	3	sequence	sequence	NN
cord-324094-23kzr8rq	23	4	based	base	VBN
cord-324094-23kzr8rq	23	5	amplifi	amplifi	NNP
cord-324094-23kzr8rq	23	6	cation	cation	NNP
cord-324094-23kzr8rq	23	7	(	(	-LRB-
cord-324094-23kzr8rq	23	8	NASBA	NASBA	NNP
cord-324094-23kzr8rq	23	9	)	)	-RRB-
cord-324094-23kzr8rq	23	10	,	,	,
cord-324094-23kzr8rq	23	11	Self	self	NN
cord-324094-23kzr8rq	23	12	-	-	HYPH
cord-324094-23kzr8rq	23	13	sustained	sustain	VBN
cord-324094-23kzr8rq	23	14	sequence	sequence	NN
cord-324094-23kzr8rq	23	15	replication	replication	NN
cord-324094-23kzr8rq	23	16	(	(	-LRB-
cord-324094-23kzr8rq	23	17	3SR	3sr	CD
cord-324094-23kzr8rq	23	18	)	)	-RRB-
cord-324094-23kzr8rq	24	1	,	,	,
cord-324094-23kzr8rq	24	2	Strand	strand	NN
cord-324094-23kzr8rq	24	3	displacement	displacement	NN
cord-324094-23kzr8rq	24	4	amplifi	amplifi	NNP
cord-324094-23kzr8rq	24	5	cation	cation	NN
cord-324094-23kzr8rq	24	6	(	(	-LRB-
cord-324094-23kzr8rq	24	7	SDA	SDA	NNP
cord-324094-23kzr8rq	24	8	)	)	-RRB-
cord-324094-23kzr8rq	24	9	as	as	RB
cord-324094-23kzr8rq	24	10	well	well	RB
cord-324094-23kzr8rq	24	11	as	as	IN
cord-324094-23kzr8rq	24	12	Polymerase	polymerase	NN
cord-324094-23kzr8rq	24	13	chain	chain	NN
cord-324094-23kzr8rq	24	14	reaction	reaction	NN
cord-324094-23kzr8rq	24	15	(	(	-LRB-
cord-324094-23kzr8rq	24	16	PCR	PCR	NNP
cord-324094-23kzr8rq	24	17	)	)	-RRB-
cord-324094-23kzr8rq	24	18	(	(	-LRB-
cord-324094-23kzr8rq	24	19	Chan	Chan	NNP
cord-324094-23kzr8rq	24	20	and	and	CC
cord-324094-23kzr8rq	24	21	Fox	Fox	NNP
cord-324094-23kzr8rq	24	22	1999	1999	CD
cord-324094-23kzr8rq	24	23	)	)	-RRB-
cord-324094-23kzr8rq	24	24	.	.	.
cord-324094-23kzr8rq	25	1	These	these	DT
cord-324094-23kzr8rq	25	2	methods	method	NNS
cord-324094-23kzr8rq	25	3	can	can	MD
cord-324094-23kzr8rq	25	4	amplify	amplify	VB
cord-324094-23kzr8rq	25	5	target	target	NN
cord-324094-23kzr8rq	25	6	nucleic	nucleic	JJ
cord-324094-23kzr8rq	25	7	acids	acid	NNS
cord-324094-23kzr8rq	25	8	to	to	IN
cord-324094-23kzr8rq	25	9	a	a	DT
cord-324094-23kzr8rq	25	10	similar	similar	JJ
cord-324094-23kzr8rq	25	11	magnitude	magnitude	NN
cord-324094-23kzr8rq	25	12	,	,	,
cord-324094-23kzr8rq	25	13	all	all	RB
cord-324094-23kzr8rq	25	14	with	with	IN
cord-324094-23kzr8rq	25	15	a	a	DT
cord-324094-23kzr8rq	25	16	detection	detection	NN
cord-324094-23kzr8rq	25	17	limit	limit	NN
cord-324094-23kzr8rq	25	18	of	of	IN
cord-324094-23kzr8rq	25	19	less	less	JJR
cord-324094-23kzr8rq	25	20	than	than	IN
cord-324094-23kzr8rq	25	21	10	10	CD
cord-324094-23kzr8rq	25	22	copies	copy	NNS
cord-324094-23kzr8rq	25	23	and	and	CC
cord-324094-23kzr8rq	25	24	within	within	IN
cord-324094-23kzr8rq	25	25	a	a	DT
cord-324094-23kzr8rq	25	26	hour	hour	NN
cord-324094-23kzr8rq	25	27	or	or	CC
cord-324094-23kzr8rq	25	28	so	so	RB
cord-324094-23kzr8rq	25	29	.	.	.
cord-324094-23kzr8rq	26	1	Although	although	IN
cord-324094-23kzr8rq	26	2	several	several	JJ
cord-324094-23kzr8rq	26	3	amplifi	amplifi	NNP
cord-324094-23kzr8rq	26	4	cation	cation	NN
cord-324094-23kzr8rq	26	5	methods	method	NNS
cord-324094-23kzr8rq	26	6	have	have	VBP
cord-324094-23kzr8rq	26	7	been	be	VBN
cord-324094-23kzr8rq	26	8	developed	develop	VBN
cord-324094-23kzr8rq	26	9	,	,	,
cord-324094-23kzr8rq	26	10	PCR	PCR	NNP
cord-324094-23kzr8rq	26	11	is	be	VBZ
cord-324094-23kzr8rq	26	12	the	the	DT
cord-324094-23kzr8rq	26	13	most	most	RBS
cord-324094-23kzr8rq	26	14	widely	widely	RB
cord-324094-23kzr8rq	26	15	used	use	VBN
cord-324094-23kzr8rq	26	16	because	because	IN
cord-324094-23kzr8rq	26	17	of	of	IN
cord-324094-23kzr8rq	26	18	its	-PRON-	PRP$
cord-324094-23kzr8rq	26	19	apparent	apparent	JJ
cord-324094-23kzr8rq	26	20	high	high	JJ
cord-324094-23kzr8rq	26	21	simplicity	simplicity	NN
cord-324094-23kzr8rq	26	22	and	and	CC
cord-324094-23kzr8rq	26	23	reliability	reliability	NN
cord-324094-23kzr8rq	26	24	.	.	.
cord-324094-23kzr8rq	27	1	Routine	routine	JJ
cord-324094-23kzr8rq	27	2	use	use	NN
cord-324094-23kzr8rq	27	3	The	the	DT
cord-324094-23kzr8rq	27	4	development	development	NN
cord-324094-23kzr8rq	27	5	of	of	IN
cord-324094-23kzr8rq	27	6	technologies	technology	NNS
cord-324094-23kzr8rq	27	7	with	with	IN
cord-324094-23kzr8rq	27	8	rapid	rapid	JJ
cord-324094-23kzr8rq	27	9	and	and	CC
cord-324094-23kzr8rq	27	10	sensitive	sensitive	JJ
cord-324094-23kzr8rq	27	11	detection	detection	NN
cord-324094-23kzr8rq	27	12	capabilities	capability	NNS
cord-324094-23kzr8rq	27	13	and	and	CC
cord-324094-23kzr8rq	27	14	increased	increase	VBN
cord-324094-23kzr8rq	27	15	throughput	throughput	NN
cord-324094-23kzr8rq	27	16	have	have	VBP
cord-324094-23kzr8rq	27	17	become	become	VBN
cord-324094-23kzr8rq	27	18	crucial	crucial	JJ
cord-324094-23kzr8rq	27	19	for	for	IN
cord-324094-23kzr8rq	27	20	responding	respond	VBG
cord-324094-23kzr8rq	27	21	to	to	IN
cord-324094-23kzr8rq	27	22	greater	great	JJR
cord-324094-23kzr8rq	27	23	number	number	NN
cord-324094-23kzr8rq	27	24	threats	threat	NNS
cord-324094-23kzr8rq	27	25	posed	pose	VBN
cord-324094-23kzr8rq	27	26	by	by	IN
cord-324094-23kzr8rq	27	27	emerging	emerge	VBG
cord-324094-23kzr8rq	27	28	and	and	CC
cord-324094-23kzr8rq	27	29	re	re	VB
cord-324094-23kzr8rq	27	30	-	-	JJ
cord-324094-23kzr8rq	27	31	emerging	emerge	VBG
cord-324094-23kzr8rq	27	32	viruses	virus	NNS
cord-324094-23kzr8rq	27	33	in	in	IN
cord-324094-23kzr8rq	27	34	the	the	DT
cord-324094-23kzr8rq	27	35	recent	recent	JJ
cord-324094-23kzr8rq	27	36	past	past	NN
cord-324094-23kzr8rq	27	37	.	.	.
cord-324094-23kzr8rq	28	1	The	the	DT
cord-324094-23kzr8rq	28	2	conventional	conventional	JJ
cord-324094-23kzr8rq	28	3	identifi	identifi	NN
cord-324094-23kzr8rq	28	4	cation	cation	NN
cord-324094-23kzr8rq	28	5	methods	method	NNS
cord-324094-23kzr8rq	28	6	require	require	VBP
cord-324094-23kzr8rq	28	7	time	time	NN
cord-324094-23kzr8rq	28	8	-	-	HYPH
cord-324094-23kzr8rq	28	9	consuming	consume	VBG
cord-324094-23kzr8rq	28	10	culturing	culturing	NN
cord-324094-23kzr8rq	28	11	,	,	,
cord-324094-23kzr8rq	28	12	and/	and/	CD
cord-324094-23kzr8rq	28	13	or	or	CC
cord-324094-23kzr8rq	28	14	detection	detection	NN
cord-324094-23kzr8rq	28	15	of	of	IN
cord-324094-23kzr8rq	28	16	antibodies	antibody	NNS
cord-324094-23kzr8rq	28	17	,	,	,
cord-324094-23kzr8rq	28	18	which	which	WDT
cord-324094-23kzr8rq	28	19	are	be	VBP
cord-324094-23kzr8rq	28	20	not	not	RB
cord-324094-23kzr8rq	28	21	very	very	RB
cord-324094-23kzr8rq	28	22	sensitive	sensitive	JJ
cord-324094-23kzr8rq	28	23	and	and	CC
cord-324094-23kzr8rq	28	24	specifi	specifi	JJ
cord-324094-23kzr8rq	28	25	c.	c.	NN
cord-324094-23kzr8rq	29	1	The	the	DT
cord-324094-23kzr8rq	29	2	recent	recent	JJ
cord-324094-23kzr8rq	29	3	advances	advance	NNS
cord-324094-23kzr8rq	29	4	in	in	IN
cord-324094-23kzr8rq	29	5	molecular	molecular	JJ
cord-324094-23kzr8rq	29	6	biology	biology	NN
cord-324094-23kzr8rq	29	7	techniques	technique	NNS
cord-324094-23kzr8rq	29	8	in	in	IN
cord-324094-23kzr8rq	29	9	the	the	DT
cord-324094-23kzr8rq	29	10	fi	fi	NNP
cord-324094-23kzr8rq	29	11	eld	eld	NN
cord-324094-23kzr8rq	29	12	of	of	IN
cord-324094-23kzr8rq	29	13	genomics	genomic	NNS
cord-324094-23kzr8rq	29	14	and	and	CC
cord-324094-23kzr8rq	29	15	proteomics	proteomic	NNS
cord-324094-23kzr8rq	29	16	greatly	greatly	RB
cord-324094-23kzr8rq	29	17	facilitate	facilitate	VBP
cord-324094-23kzr8rq	29	18	the	the	DT
cord-324094-23kzr8rq	29	19	rapid	rapid	JJ
cord-324094-23kzr8rq	29	20	identifi	identifi	NNP
cord-324094-23kzr8rq	29	21	cation	cation	NN
cord-324094-23kzr8rq	29	22	with	with	IN
cord-324094-23kzr8rq	29	23	more	more	JJR
cord-324094-23kzr8rq	29	24	accuracy	accuracy	NN
cord-324094-23kzr8rq	29	25	.	.	.
cord-324094-23kzr8rq	30	1	We	-PRON-	PRP
cord-324094-23kzr8rq	30	2	have	have	VBP
cord-324094-23kzr8rq	30	3	developed	develop	VBN
cord-324094-23kzr8rq	30	4	two	two	CD
cord-324094-23kzr8rq	30	5	real	real	JJ
cord-324094-23kzr8rq	30	6	-	-	HYPH
cord-324094-23kzr8rq	30	7	time	time	NN
cord-324094-23kzr8rq	30	8	assays	assay	NNS
cord-324094-23kzr8rq	30	9	ie	ie	FW
cord-324094-23kzr8rq	30	10	.	.	.
cord-324094-23kzr8rq	31	1	,	,	,
cord-324094-23kzr8rq	31	2	SYBR	SYBR	NNP
cord-324094-23kzr8rq	31	3	green	green	JJ
cord-324094-23kzr8rq	32	1	I	-PRON-	PRP
cord-324094-23kzr8rq	32	2	based	base	VBD
cord-324094-23kzr8rq	32	3	real	real	JJ
cord-324094-23kzr8rq	32	4	time	time	NN
cord-324094-23kzr8rq	32	5	reverse	reverse	NN
cord-324094-23kzr8rq	32	6	transcription	transcription	NN
cord-324094-23kzr8rq	32	7	polymerase	polymerase	NN
cord-324094-23kzr8rq	32	8	chain	chain	NN
cord-324094-23kzr8rq	32	9	reaction	reaction	NN
cord-324094-23kzr8rq	32	10	(	(	-LRB-
cord-324094-23kzr8rq	32	11	RT	RT	NNP
cord-324094-23kzr8rq	32	12	-	-	HYPH
cord-324094-23kzr8rq	32	13	PCR	PCR	NNP
cord-324094-23kzr8rq	32	14	)	)	-RRB-
cord-324094-23kzr8rq	32	15	and	and	CC
cord-324094-23kzr8rq	32	16	RT	rt	NN
cord-324094-23kzr8rq	32	17	-	-	HYPH
cord-324094-23kzr8rq	32	18	loop	loop	NN
cord-324094-23kzr8rq	32	19	-	-	HYPH
cord-324094-23kzr8rq	32	20	mediated	mediate	VBN
cord-324094-23kzr8rq	32	21	isothermal	isothermal	JJ
cord-324094-23kzr8rq	32	22	amplifi	amplifi	NNP
cord-324094-23kzr8rq	32	23	cation	cation	NN
cord-324094-23kzr8rq	32	24	(	(	-LRB-
cord-324094-23kzr8rq	32	25	LAMP	LAMP	NNP
cord-324094-23kzr8rq	32	26	)	)	-RRB-
cord-324094-23kzr8rq	32	27	assay	assay	NN
cord-324094-23kzr8rq	32	28	for	for	IN
cord-324094-23kzr8rq	32	29	rapid	rapid	JJ
cord-324094-23kzr8rq	32	30	detection	detection	NN
cord-324094-23kzr8rq	32	31	as	as	RB
cord-324094-23kzr8rq	32	32	well	well	RB
cord-324094-23kzr8rq	32	33	as	as	IN
cord-324094-23kzr8rq	32	34	typing	typing	NN
cord-324094-23kzr8rq	32	35	of	of	IN
cord-324094-23kzr8rq	32	36	some	some	DT
cord-324094-23kzr8rq	32	37	of	of	IN
cord-324094-23kzr8rq	32	38	the	the	DT
cord-324094-23kzr8rq	32	39	emerging	emerge	VBG
cord-324094-23kzr8rq	32	40	viruses	virus	NNS
cord-324094-23kzr8rq	32	41	of	of	IN
cord-324094-23kzr8rq	32	42	biomedical	biomedical	JJ
cord-324094-23kzr8rq	32	43	importance	importance	NN
cord-324094-23kzr8rq	32	44	viz	viz	NN
cord-324094-23kzr8rq	32	45	.	.	.
cord-324094-23kzr8rq	33	1	dengue	dengue	NN
cord-324094-23kzr8rq	33	2	,	,	,
cord-324094-23kzr8rq	33	3	Japanese	japanese	JJ
cord-324094-23kzr8rq	33	4	encephalitis	encephalitis	RB
cord-324094-23kzr8rq	33	5	,	,	,
cord-324094-23kzr8rq	33	6	chikungunya	chikungunya	NN
cord-324094-23kzr8rq	33	7	,	,	,
cord-324094-23kzr8rq	33	8	west	west	NNP
cord-324094-23kzr8rq	33	9	Nile	Nile	NNP
cord-324094-23kzr8rq	33	10	,	,	,
cord-324094-23kzr8rq	33	11	severe	severe	JJ
cord-324094-23kzr8rq	33	12	acute	acute	JJ
cord-324094-23kzr8rq	33	13	respiratory	respiratory	JJ
cord-324094-23kzr8rq	33	14	syndrome	syndrome	NN
cord-324094-23kzr8rq	33	15	virus	virus	NN
cord-324094-23kzr8rq	33	16	(	(	-LRB-
cord-324094-23kzr8rq	33	17	SARS	SARS	NNP
cord-324094-23kzr8rq	33	18	)	)	-RRB-
cord-324094-23kzr8rq	33	19	etc	etc	FW
cord-324094-23kzr8rq	33	20	.	.	.
cord-324094-23kzr8rq	34	1	Both	both	CC
cord-324094-23kzr8rq	34	2	these	these	DT
cord-324094-23kzr8rq	34	3	techniques	technique	NNS
cord-324094-23kzr8rq	34	4	are	be	VBP
cord-324094-23kzr8rq	34	5	capable	capable	JJ
cord-324094-23kzr8rq	34	6	of	of	IN
cord-324094-23kzr8rq	34	7	detection	detection	NN
cord-324094-23kzr8rq	34	8	and	and	CC
cord-324094-23kzr8rq	34	9	differentiation	differentiation	NN
cord-324094-23kzr8rq	34	10	as	as	RB
cord-324094-23kzr8rq	34	11	well	well	RB
cord-324094-23kzr8rq	34	12	as	as	IN
cord-324094-23kzr8rq	34	13	quantifying	quantify	VBG
cord-324094-23kzr8rq	34	14	viral	viral	JJ
cord-324094-23kzr8rq	34	15	load	load	NN
cord-324094-23kzr8rq	34	16	with	with	IN
cord-324094-23kzr8rq	34	17	higher	high	JJR
cord-324094-23kzr8rq	34	18	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	34	19	,	,	,
cord-324094-23kzr8rq	34	20	rapidity	rapidity	NN
cord-324094-23kzr8rq	34	21	,	,	,
cord-324094-23kzr8rq	34	22	specifi	specifi	VBN
cord-324094-23kzr8rq	34	23	city	city	NN
cord-324094-23kzr8rq	34	24	.	.	.
cord-324094-23kzr8rq	35	1	One	one	CD
cord-324094-23kzr8rq	35	2	of	of	IN
cord-324094-23kzr8rq	35	3	the	the	DT
cord-324094-23kzr8rq	35	4	most	most	RBS
cord-324094-23kzr8rq	35	5	important	important	JJ
cord-324094-23kzr8rq	35	6	advantages	advantage	NNS
cord-324094-23kzr8rq	35	7	of	of	IN
cord-324094-23kzr8rq	35	8	LAMP	LAMP	NNP
cord-324094-23kzr8rq	35	9	is	be	VBZ
cord-324094-23kzr8rq	35	10	its	-PRON-	PRP$
cord-324094-23kzr8rq	35	11	fi	fi	NN
cord-324094-23kzr8rq	35	12	eld	eld	NN
cord-324094-23kzr8rq	35	13	applicability	applicability	NN
cord-324094-23kzr8rq	35	14	,	,	,
cord-324094-23kzr8rq	35	15	without	without	IN
cord-324094-23kzr8rq	35	16	requirement	requirement	NN
cord-324094-23kzr8rq	35	17	of	of	IN
cord-324094-23kzr8rq	35	18	any	any	DT
cord-324094-23kzr8rq	35	19	sophisticated	sophisticated	JJ
cord-324094-23kzr8rq	35	20	equipments	equipment	NNS
cord-324094-23kzr8rq	35	21	.	.	.
cord-324094-23kzr8rq	36	1	Both	both	CC
cord-324094-23kzr8rq	36	2	these	these	DT
cord-324094-23kzr8rq	36	3	assays	assay	NNS
cord-324094-23kzr8rq	36	4	have	have	VBP
cord-324094-23kzr8rq	36	5	been	be	VBN
cord-324094-23kzr8rq	36	6	extensively	extensively	RB
cord-324094-23kzr8rq	36	7	evaluated	evaluate	VBN
cord-324094-23kzr8rq	36	8	and	and	CC
cord-324094-23kzr8rq	36	9	validated	validate	VBN
cord-324094-23kzr8rq	36	10	with	with	IN
cord-324094-23kzr8rq	36	11	clinical	clinical	JJ
cord-324094-23kzr8rq	36	12	samples	sample	NNS
cord-324094-23kzr8rq	36	13	of	of	IN
cord-324094-23kzr8rq	36	14	recent	recent	JJ
cord-324094-23kzr8rq	36	15	epidemics	epidemic	NNS
cord-324094-23kzr8rq	36	16	from	from	IN
cord-324094-23kzr8rq	36	17	different	different	JJ
cord-324094-23kzr8rq	36	18	parts	part	NNS
cord-324094-23kzr8rq	36	19	of	of	IN
cord-324094-23kzr8rq	36	20	India	India	NNP
cord-324094-23kzr8rq	36	21	.	.	.
cord-324094-23kzr8rq	37	1	The	the	DT
cord-324094-23kzr8rq	37	2	establishment	establishment	NN
cord-324094-23kzr8rq	37	3	of	of	IN
cord-324094-23kzr8rq	37	4	these	these	DT
cord-324094-23kzr8rq	37	5	real	real	JJ
cord-324094-23kzr8rq	37	6	time	time	NN
cord-324094-23kzr8rq	37	7	molecular	molecular	JJ
cord-324094-23kzr8rq	37	8	assays	assay	NNS
cord-324094-23kzr8rq	37	9	will	will	MD
cord-324094-23kzr8rq	37	10	certainly	certainly	RB
cord-324094-23kzr8rq	37	11	facilitate	facilitate	VB
cord-324094-23kzr8rq	37	12	the	the	DT
cord-324094-23kzr8rq	37	13	rapid	rapid	JJ
cord-324094-23kzr8rq	37	14	detection	detection	NN
cord-324094-23kzr8rq	37	15	of	of	IN
cord-324094-23kzr8rq	37	16	viruses	virus	NNS
cord-324094-23kzr8rq	37	17	with	with	IN
cord-324094-23kzr8rq	37	18	high	high	JJ
cord-324094-23kzr8rq	37	19	degree	degree	NN
cord-324094-23kzr8rq	37	20	of	of	IN
cord-324094-23kzr8rq	37	21	precision	precision	NN
cord-324094-23kzr8rq	37	22	and	and	CC
cord-324094-23kzr8rq	37	23	accuracy	accuracy	NN
cord-324094-23kzr8rq	37	24	in	in	IN
cord-324094-23kzr8rq	37	25	future	future	NN
cord-324094-23kzr8rq	37	26	.	.	.
cord-324094-23kzr8rq	38	1	[	[	-LRB-
cord-324094-23kzr8rq	38	2	Parida	Parida	NNP
cord-324094-23kzr8rq	38	3	M	M	NNP
cord-324094-23kzr8rq	38	4	M	M	NNP
cord-324094-23kzr8rq	38	5	2008	2008	CD
cord-324094-23kzr8rq	38	6	Rapid	Rapid	NNP
cord-324094-23kzr8rq	38	7	and	and	CC
cord-324094-23kzr8rq	38	8	real	real	JJ
cord-324094-23kzr8rq	38	9	-	-	HYPH
cord-324094-23kzr8rq	38	10	time	time	NN
cord-324094-23kzr8rq	38	11	detection	detection	NN
cord-324094-23kzr8rq	38	12	technologies	technology	NNS
cord-324094-23kzr8rq	38	13	for	for	IN
cord-324094-23kzr8rq	38	14	emerging	emerge	VBG
cord-324094-23kzr8rq	38	15	viruses	virus	NNS
cord-324094-23kzr8rq	38	16	of	of	IN
cord-324094-23kzr8rq	38	17	biomedical	biomedical	JJ
cord-324094-23kzr8rq	38	18	importance	importance	NN
cord-324094-23kzr8rq	38	19	;	;	:
cord-324094-23kzr8rq	38	20	J.	J.	NNP
cord-324094-23kzr8rq	38	21	Biosci	Biosci	NNP
cord-324094-23kzr8rq	38	22	.	.	.
cord-324094-23kzr8rq	39	1	33	33	CD
cord-324094-23kzr8rq	39	2	617	617	CD
cord-324094-23kzr8rq	39	3	-	-	SYM
cord-324094-23kzr8rq	39	4	628	628	CD
cord-324094-23kzr8rq	39	5	]	]	-RRB-
cord-324094-23kzr8rq	39	6	]	]	-RRB-
cord-324094-23kzr8rq	39	7	of	of	IN
cord-324094-23kzr8rq	39	8	PCR	PCR	NNP
cord-324094-23kzr8rq	39	9	as	as	IN
cord-324094-23kzr8rq	39	10	a	a	DT
cord-324094-23kzr8rq	39	11	standard	standard	JJ
cord-324094-23kzr8rq	39	12	approach	approach	NN
cord-324094-23kzr8rq	39	13	in	in	IN
cord-324094-23kzr8rq	39	14	biotechnology	biotechnology	NN
cord-324094-23kzr8rq	39	15	and	and	CC
cord-324094-23kzr8rq	39	16	medical	medical	JJ
cord-324094-23kzr8rq	39	17	diagnostic	diagnostic	JJ
cord-324094-23kzr8rq	39	18	laboratories	laboratory	NNS
cord-324094-23kzr8rq	39	19	has	have	VBZ
cord-324094-23kzr8rq	39	20	been	be	VBN
cord-324094-23kzr8rq	39	21	usually	usually	RB
cord-324094-23kzr8rq	39	22	practiced	practice	VBN
cord-324094-23kzr8rq	39	23	.	.	.
cord-324094-23kzr8rq	40	1	During	during	IN
cord-324094-23kzr8rq	40	2	the	the	DT
cord-324094-23kzr8rq	40	3	past	past	JJ
cord-324094-23kzr8rq	40	4	decade	decade	NN
cord-324094-23kzr8rq	40	5	,	,	,
cord-324094-23kzr8rq	40	6	various	various	JJ
cord-324094-23kzr8rq	40	7	forms	form	NNS
cord-324094-23kzr8rq	40	8	of	of	IN
cord-324094-23kzr8rq	40	9	PCRs	pcr	NNS
cord-324094-23kzr8rq	40	10	such	such	JJ
cord-324094-23kzr8rq	40	11	as	as	IN
cord-324094-23kzr8rq	40	12	reverse	reverse	JJ
cord-324094-23kzr8rq	40	13	transcription	transcription	NN
cord-324094-23kzr8rq	40	14	polymerase	polymerase	NN
cord-324094-23kzr8rq	40	15	chain	chain	NN
cord-324094-23kzr8rq	40	16	reaction	reaction	NN
cord-324094-23kzr8rq	40	17	(	(	-LRB-
cord-324094-23kzr8rq	40	18	RT	RT	NNP
cord-324094-23kzr8rq	40	19	-	-	HYPH
cord-324094-23kzr8rq	40	20	PCR	PCR	NNP
cord-324094-23kzr8rq	40	21	)	)	-RRB-
cord-324094-23kzr8rq	40	22	,	,	,
cord-324094-23kzr8rq	40	23	Nested	Nested	NNP
cord-324094-23kzr8rq	40	24	PCR	PCR	NNP
cord-324094-23kzr8rq	40	25	and	and	CC
cord-324094-23kzr8rq	40	26	Multiplex	Multiplex	NNP
cord-324094-23kzr8rq	40	27	PCR	PCR	NNP
cord-324094-23kzr8rq	40	28	have	have	VBP
cord-324094-23kzr8rq	40	29	been	be	VBN
cord-324094-23kzr8rq	40	30	developed	develop	VBN
cord-324094-23kzr8rq	40	31	to	to	TO
cord-324094-23kzr8rq	40	32	address	address	VB
cord-324094-23kzr8rq	40	33	the	the	DT
cord-324094-23kzr8rq	40	34	need	need	NN
cord-324094-23kzr8rq	40	35	for	for	IN
cord-324094-23kzr8rq	40	36	rapid	rapid	JJ
cord-324094-23kzr8rq	40	37	identifi	identifi	NNP
cord-324094-23kzr8rq	40	38	cation	cation	NN
cord-324094-23kzr8rq	40	39	of	of	IN
cord-324094-23kzr8rq	40	40	viruses	virus	NNS
cord-324094-23kzr8rq	40	41	to	to	IN
cord-324094-23kzr8rq	40	42	serotype	serotype	JJ
cord-324094-23kzr8rq	40	43	level	level	NN
cord-324094-23kzr8rq	40	44	with	with	IN
cord-324094-23kzr8rq	40	45	more	more	JJR
cord-324094-23kzr8rq	40	46	accuracy	accuracy	NN
cord-324094-23kzr8rq	41	1	(	(	-LRB-
cord-324094-23kzr8rq	41	2	Ratcliff	Ratcliff	NNP
cord-324094-23kzr8rq	41	3	et	et	NNP
cord-324094-23kzr8rq	41	4	al	al	NNP
cord-324094-23kzr8rq	41	5	2007	2007	CD
cord-324094-23kzr8rq	41	6	)	)	-RRB-
cord-324094-23kzr8rq	41	7	.	.	.
cord-324094-23kzr8rq	42	1	Despite	despite	IN
cord-324094-23kzr8rq	42	2	the	the	DT
cord-324094-23kzr8rq	42	3	obtainable	obtainable	JJ
cord-324094-23kzr8rq	42	4	magnitude	magnitude	NN
cord-324094-23kzr8rq	42	5	of	of	IN
cord-324094-23kzr8rq	42	6	amplifi	amplifi	NNP
cord-324094-23kzr8rq	42	7	cation	cation	NN
cord-324094-23kzr8rq	42	8	,	,	,
cord-324094-23kzr8rq	42	9	these	these	DT
cord-324094-23kzr8rq	42	10	PCR	PCR	NNP
cord-324094-23kzr8rq	42	11	based	base	VBN
cord-324094-23kzr8rq	42	12	methods	method	NNS
cord-324094-23kzr8rq	42	13	require	require	VBP
cord-324094-23kzr8rq	42	14	either	either	CC
cord-324094-23kzr8rq	42	15	high	high	JJ
cord-324094-23kzr8rq	42	16	precision	precision	NN
cord-324094-23kzr8rq	42	17	instruments	instrument	NNS
cord-324094-23kzr8rq	42	18	for	for	IN
cord-324094-23kzr8rq	42	19	the	the	DT
cord-324094-23kzr8rq	42	20	amplifi	amplifi	NNP
cord-324094-23kzr8rq	42	21	cation	cation	NN
cord-324094-23kzr8rq	42	22	or	or	CC
cord-324094-23kzr8rq	42	23	elaborate	elaborate	JJ
cord-324094-23kzr8rq	42	24	methods	method	NNS
cord-324094-23kzr8rq	42	25	for	for	IN
cord-324094-23kzr8rq	42	26	detection	detection	NN
cord-324094-23kzr8rq	42	27	of	of	IN
cord-324094-23kzr8rq	42	28	the	the	DT
cord-324094-23kzr8rq	42	29	amplifi	amplifi	NNP
cord-324094-23kzr8rq	42	30	ed	ed	NNP
cord-324094-23kzr8rq	42	31	products	product	NNS
cord-324094-23kzr8rq	42	32	.	.	.
cord-324094-23kzr8rq	43	1	In	in	IN
cord-324094-23kzr8rq	43	2	addition	addition	NN
cord-324094-23kzr8rq	43	3	,	,	,
cord-324094-23kzr8rq	43	4	these	these	DT
cord-324094-23kzr8rq	43	5	methods	method	NNS
cord-324094-23kzr8rq	43	6	are	be	VBP
cord-324094-23kzr8rq	43	7	often	often	RB
cord-324094-23kzr8rq	43	8	cumbersome	cumbersome	JJ
cord-324094-23kzr8rq	43	9	to	to	TO
cord-324094-23kzr8rq	43	10	adapt	adapt	VB
cord-324094-23kzr8rq	43	11	for	for	IN
cord-324094-23kzr8rq	43	12	routine	routine	JJ
cord-324094-23kzr8rq	43	13	clinical	clinical	JJ
cord-324094-23kzr8rq	43	14	use	use	NN
cord-324094-23kzr8rq	43	15	especially	especially	RB
cord-324094-23kzr8rq	43	16	in	in	IN
cord-324094-23kzr8rq	43	17	peripheral	peripheral	JJ
cord-324094-23kzr8rq	43	18	health	health	NN
cord-324094-23kzr8rq	43	19	care	care	NN
cord-324094-23kzr8rq	43	20	settings	setting	NNS
cord-324094-23kzr8rq	43	21	and	and	CC
cord-324094-23kzr8rq	43	22	private	private	JJ
cord-324094-23kzr8rq	43	23	clinics	clinic	NNS
cord-324094-23kzr8rq	43	24	.	.	.
cord-324094-23kzr8rq	44	1	In	in	IN
cord-324094-23kzr8rq	44	2	addition	addition	NN
cord-324094-23kzr8rq	44	3	,	,	,
cord-324094-23kzr8rq	44	4	the	the	DT
cord-324094-23kzr8rq	44	5	PCR	PCR	NNP
cord-324094-23kzr8rq	44	6	method	method	NN
cord-324094-23kzr8rq	44	7	has	have	VBZ
cord-324094-23kzr8rq	44	8	several	several	JJ
cord-324094-23kzr8rq	44	9	intrinsic	intrinsic	JJ
cord-324094-23kzr8rq	44	10	disadvantages	disadvantage	NNS
cord-324094-23kzr8rq	44	11	,	,	,
cord-324094-23kzr8rq	44	12	such	such	JJ
cord-324094-23kzr8rq	44	13	as	as	IN
cord-324094-23kzr8rq	44	14	the	the	DT
cord-324094-23kzr8rq	44	15	requirement	requirement	NN
cord-324094-23kzr8rq	44	16	of	of	IN
cord-324094-23kzr8rq	44	17	thermal	thermal	JJ
cord-324094-23kzr8rq	44	18	cycling	cycling	NN
cord-324094-23kzr8rq	44	19	,	,	,
cord-324094-23kzr8rq	44	20	insuffi	insuffi	NNP
cord-324094-23kzr8rq	44	21	cient	cient	NNP
cord-324094-23kzr8rq	44	22	specifi	specifi	NNP
cord-324094-23kzr8rq	44	23	city	city	NNP
cord-324094-23kzr8rq	44	24	and	and	CC
cord-324094-23kzr8rq	44	25	rather	rather	RB
cord-324094-23kzr8rq	44	26	low	low	JJ
cord-324094-23kzr8rq	44	27	amplifi	amplifi	NNP
cord-324094-23kzr8rq	44	28	cations	cation	NNS
cord-324094-23kzr8rq	44	29	effi	effi	NNP
cord-324094-23kzr8rq	44	30	ciency	ciency	NNP
cord-324094-23kzr8rq	44	31	.	.	.
cord-324094-23kzr8rq	45	1	More	more	RBR
cord-324094-23kzr8rq	45	2	sensitive	sensitive	JJ
cord-324094-23kzr8rq	45	3	and	and	CC
cord-324094-23kzr8rq	45	4	real	real	JJ
cord-324094-23kzr8rq	45	5	time	time	NN
cord-324094-23kzr8rq	45	6	based	base	VBN
cord-324094-23kzr8rq	45	7	assays	assay	NNS
cord-324094-23kzr8rq	45	8	are	be	VBP
cord-324094-23kzr8rq	45	9	therefore	therefore	RB
cord-324094-23kzr8rq	45	10	needed	need	VBN
cord-324094-23kzr8rq	45	11	to	to	TO
cord-324094-23kzr8rq	45	12	complement	complement	VB
cord-324094-23kzr8rq	45	13	the	the	DT
cord-324094-23kzr8rq	45	14	existing	exist	VBG
cord-324094-23kzr8rq	45	15	PCR	PCR	NNP
cord-324094-23kzr8rq	45	16	based	base	VBN
cord-324094-23kzr8rq	45	17	assay	assay	NN
cord-324094-23kzr8rq	45	18	systems	system	NNS
cord-324094-23kzr8rq	45	19	.	.	.
cord-324094-23kzr8rq	46	1	The	the	DT
cord-324094-23kzr8rq	46	2	Real	real	JJ
cord-324094-23kzr8rq	46	3	-	-	HYPH
cord-324094-23kzr8rq	46	4	time	time	NN
cord-324094-23kzr8rq	46	5	PCR	PCR	NNP
cord-324094-23kzr8rq	46	6	assay	assay	NN
cord-324094-23kzr8rq	46	7	has	have	VBZ
cord-324094-23kzr8rq	46	8	many	many	JJ
cord-324094-23kzr8rq	46	9	advantages	advantage	NNS
cord-324094-23kzr8rq	46	10	over	over	IN
cord-324094-23kzr8rq	46	11	conventional	conventional	JJ
cord-324094-23kzr8rq	46	12	PCR	pcr	NN
cord-324094-23kzr8rq	46	13	methods	method	NNS
cord-324094-23kzr8rq	46	14	,	,	,
cord-324094-23kzr8rq	46	15	including	include	VBG
cord-324094-23kzr8rq	46	16	rapidity	rapidity	NN
cord-324094-23kzr8rq	46	17	,	,	,
cord-324094-23kzr8rq	46	18	quantitative	quantitative	JJ
cord-324094-23kzr8rq	46	19	measurement	measurement	NN
cord-324094-23kzr8rq	46	20	,	,	,
cord-324094-23kzr8rq	46	21	lower	low	JJR
cord-324094-23kzr8rq	46	22	contamination	contamination	NN
cord-324094-23kzr8rq	46	23	rate	rate	NN
cord-324094-23kzr8rq	46	24	,	,	,
cord-324094-23kzr8rq	46	25	higher	high	JJR
cord-324094-23kzr8rq	46	26	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	46	27	,	,	,
cord-324094-23kzr8rq	46	28	higher	high	JJR
cord-324094-23kzr8rq	46	29	specifi	specifi	NN
cord-324094-23kzr8rq	46	30	city	city	NN
cord-324094-23kzr8rq	46	31	,	,	,
cord-324094-23kzr8rq	46	32	and	and	CC
cord-324094-23kzr8rq	46	33	easy	easy	JJ
cord-324094-23kzr8rq	46	34	standardization	standardization	NN
cord-324094-23kzr8rq	46	35	.	.	.
cord-324094-23kzr8rq	47	1	Thus	thus	RB
cord-324094-23kzr8rq	47	2	,	,	,
cord-324094-23kzr8rq	47	3	nucleic	nucleic	JJ
cord-324094-23kzr8rq	47	4	acid	acid	NN
cord-324094-23kzr8rq	47	5	-	-	HYPH
cord-324094-23kzr8rq	47	6	based	base	VBN
cord-324094-23kzr8rq	47	7	assays	assay	NNS
cord-324094-23kzr8rq	47	8	or	or	CC
cord-324094-23kzr8rq	47	9	real	real	JJ
cord-324094-23kzr8rq	47	10	-	-	HYPH
cord-324094-23kzr8rq	47	11	time	time	NN
cord-324094-23kzr8rq	47	12	quantitative	quantitative	JJ
cord-324094-23kzr8rq	47	13	assay	assay	NN
cord-324094-23kzr8rq	47	14	might	may	MD
cord-324094-23kzr8rq	47	15	eventually	eventually	RB
cord-324094-23kzr8rq	47	16	replace	replace	VB
cord-324094-23kzr8rq	47	17	virus	virus	NN
cord-324094-23kzr8rq	47	18	isolation	isolation	NN
cord-324094-23kzr8rq	47	19	and	and	CC
cord-324094-23kzr8rq	47	20	conventional	conventional	JJ
cord-324094-23kzr8rq	47	21	RT	RT	NNP
cord-324094-23kzr8rq	47	22	-	-	HYPH
cord-324094-23kzr8rq	47	23	PCR	PCR	NNP
cord-324094-23kzr8rq	47	24	as	as	IN
cord-324094-23kzr8rq	47	25	the	the	DT
cord-324094-23kzr8rq	47	26	new	new	JJ
cord-324094-23kzr8rq	47	27	gold	gold	NN
cord-324094-23kzr8rq	47	28	standard	standard	NN
cord-324094-23kzr8rq	47	29	for	for	IN
cord-324094-23kzr8rq	47	30	the	the	DT
cord-324094-23kzr8rq	47	31	rapid	rapid	JJ
cord-324094-23kzr8rq	47	32	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	47	33	of	of	IN
cord-324094-23kzr8rq	47	34	virus	virus	NN
cord-324094-23kzr8rq	47	35	infection	infection	NN
cord-324094-23kzr8rq	47	36	in	in	IN
cord-324094-23kzr8rq	47	37	the	the	DT
cord-324094-23kzr8rq	47	38	acute	acute	JJ
cord-324094-23kzr8rq	47	39	-	-	HYPH
cord-324094-23kzr8rq	47	40	phase	phase	NN
cord-324094-23kzr8rq	47	41	samples	sample	NNS
cord-324094-23kzr8rq	47	42	(	(	-LRB-
cord-324094-23kzr8rq	47	43	Ratcliff	Ratcliff	NNP
cord-324094-23kzr8rq	47	44	et	et	NNP
cord-324094-23kzr8rq	47	45	al	al	NNP
cord-324094-23kzr8rq	47	46	2007	2007	CD
cord-324094-23kzr8rq	47	47	)	)	-RRB-
cord-324094-23kzr8rq	47	48	.	.	.
cord-324094-23kzr8rq	48	1	Real	real	JJ
cord-324094-23kzr8rq	48	2	-	-	HYPH
cord-324094-23kzr8rq	48	3	time	time	NN
cord-324094-23kzr8rq	48	4	PCR	PCR	NNP
cord-324094-23kzr8rq	48	5	has	have	VBZ
cord-324094-23kzr8rq	48	6	enhanced	enhance	VBN
cord-324094-23kzr8rq	48	7	wider	wide	JJR
cord-324094-23kzr8rq	48	8	acceptance	acceptance	NN
cord-324094-23kzr8rq	48	9	of	of	IN
cord-324094-23kzr8rq	48	10	the	the	DT
cord-324094-23kzr8rq	48	11	PCR	PCR	NNP
cord-324094-23kzr8rq	48	12	due	due	IN
cord-324094-23kzr8rq	48	13	to	to	IN
cord-324094-23kzr8rq	48	14	its	-PRON-	PRP$
cord-324094-23kzr8rq	48	15	improved	improved	JJ
cord-324094-23kzr8rq	48	16	rapidity	rapidity	NN
cord-324094-23kzr8rq	48	17	,	,	,
cord-324094-23kzr8rq	48	18	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	48	19	,	,	,
cord-324094-23kzr8rq	48	20	reproducibility	reproducibility	NN
cord-324094-23kzr8rq	48	21	and	and	CC
cord-324094-23kzr8rq	48	22	the	the	DT
cord-324094-23kzr8rq	48	23	reduced	reduce	VBN
cord-324094-23kzr8rq	48	24	risk	risk	NN
cord-324094-23kzr8rq	48	25	of	of	IN
cord-324094-23kzr8rq	48	26	carry	carry	VB
cord-324094-23kzr8rq	48	27	-	-	HYPH
cord-324094-23kzr8rq	48	28	over	over	RP
cord-324094-23kzr8rq	48	29	contamination	contamination	NN
cord-324094-23kzr8rq	48	30	.	.	.
cord-324094-23kzr8rq	49	1	Real	real	JJ
cord-324094-23kzr8rq	49	2	-	-	HYPH
cord-324094-23kzr8rq	49	3	time	time	NN
cord-324094-23kzr8rq	49	4	PCR	PCR	NNP
cord-324094-23kzr8rq	49	5	assays	assay	NNS
cord-324094-23kzr8rq	49	6	used	use	VBN
cord-324094-23kzr8rq	49	7	for	for	IN
cord-324094-23kzr8rq	49	8	quantitative	quantitative	JJ
cord-324094-23kzr8rq	49	9	RT	RT	NNP
cord-324094-23kzr8rq	49	10	-	-	HYPH
cord-324094-23kzr8rq	49	11	PCR	PCR	NNP
cord-324094-23kzr8rq	49	12	combine	combine	VBP
cord-324094-23kzr8rq	49	13	the	the	DT
cord-324094-23kzr8rq	49	14	best	good	JJS
cord-324094-23kzr8rq	49	15	attributes	attribute	NNS
cord-324094-23kzr8rq	49	16	of	of	IN
cord-324094-23kzr8rq	49	17	both	both	DT
cord-324094-23kzr8rq	49	18	relative	relative	JJ
cord-324094-23kzr8rq	49	19	and	and	CC
cord-324094-23kzr8rq	49	20	competitive	competitive	JJ
cord-324094-23kzr8rq	49	21	(	(	-LRB-
cord-324094-23kzr8rq	49	22	end	end	NN
cord-324094-23kzr8rq	49	23	-	-	HYPH
cord-324094-23kzr8rq	49	24	point	point	NN
cord-324094-23kzr8rq	49	25	)	)	-RRB-
cord-324094-23kzr8rq	49	26	RT	RT	NNP
cord-324094-23kzr8rq	49	27	-	-	HYPH
cord-324094-23kzr8rq	49	28	PCR	PCR	NNP
cord-324094-23kzr8rq	49	29	in	in	IN
cord-324094-23kzr8rq	49	30	that	that	IN
cord-324094-23kzr8rq	49	31	they	-PRON-	PRP
cord-324094-23kzr8rq	49	32	are	be	VBP
cord-324094-23kzr8rq	49	33	accurate	accurate	JJ
cord-324094-23kzr8rq	49	34	,	,	,
cord-324094-23kzr8rq	49	35	precise	precise	JJ
cord-324094-23kzr8rq	49	36	,	,	,
cord-324094-23kzr8rq	49	37	capable	capable	JJ
cord-324094-23kzr8rq	49	38	of	of	IN
cord-324094-23kzr8rq	49	39	high	high	JJ
cord-324094-23kzr8rq	49	40	throughput	throughput	NN
cord-324094-23kzr8rq	49	41	,	,	,
cord-324094-23kzr8rq	49	42	and	and	CC
cord-324094-23kzr8rq	49	43	relatively	relatively	RB
cord-324094-23kzr8rq	49	44	easy	easy	JJ
cord-324094-23kzr8rq	49	45	to	to	TO
cord-324094-23kzr8rq	49	46	perform	perform	VB
cord-324094-23kzr8rq	49	47	.	.	.
cord-324094-23kzr8rq	50	1	The	the	DT
cord-324094-23kzr8rq	50	2	majority	majority	NN
cord-324094-23kzr8rq	50	3	of	of	IN
cord-324094-23kzr8rq	50	4	diagnostic	diagnostic	JJ
cord-324094-23kzr8rq	50	5	PCR	PCR	NNP
cord-324094-23kzr8rq	50	6	assays	assay	NNS
cord-324094-23kzr8rq	50	7	reported	report	VBN
cord-324094-23kzr8rq	50	8	to	to	IN
cord-324094-23kzr8rq	50	9	date	date	NN
cord-324094-23kzr8rq	50	10	have	have	VBP
cord-324094-23kzr8rq	50	11	been	be	VBN
cord-324094-23kzr8rq	50	12	used	use	VBN
cord-324094-23kzr8rq	50	13	in	in	IN
cord-324094-23kzr8rq	50	14	a	a	DT
cord-324094-23kzr8rq	50	15	qualitative	qualitative	JJ
cord-324094-23kzr8rq	50	16	,	,	,
cord-324094-23kzr8rq	50	17	or	or	CC
cord-324094-23kzr8rq	50	18	'	'	``
cord-324094-23kzr8rq	50	19	yes	yes	UH
cord-324094-23kzr8rq	50	20	/	/	SYM
cord-324094-23kzr8rq	50	21	no	no	UH
cord-324094-23kzr8rq	50	22	'	'	''
cord-324094-23kzr8rq	50	23	format	format	NN
cord-324094-23kzr8rq	50	24	.	.	.
cord-324094-23kzr8rq	51	1	The	the	DT
cord-324094-23kzr8rq	51	2	development	development	NN
cord-324094-23kzr8rq	51	3	of	of	IN
cord-324094-23kzr8rq	51	4	real	real	JJ
cord-324094-23kzr8rq	51	5	-	-	HYPH
cord-324094-23kzr8rq	51	6	time	time	NN
cord-324094-23kzr8rq	51	7	PCR	PCR	NNP
cord-324094-23kzr8rq	51	8	has	have	VBZ
cord-324094-23kzr8rq	51	9	brought	bring	VBN
cord-324094-23kzr8rq	51	10	true	true	JJ
cord-324094-23kzr8rq	51	11	quantitation	quantitation	NN
cord-324094-23kzr8rq	51	12	of	of	IN
cord-324094-23kzr8rq	51	13	target	target	NN
cord-324094-23kzr8rq	51	14	nucleic	nucleic	JJ
cord-324094-23kzr8rq	51	15	acids	acid	NNS
cord-324094-23kzr8rq	51	16	out	out	IN
cord-324094-23kzr8rq	51	17	of	of	IN
cord-324094-23kzr8rq	51	18	the	the	DT
cord-324094-23kzr8rq	51	19	pure	pure	JJ
cord-324094-23kzr8rq	51	20	research	research	NN
cord-324094-23kzr8rq	51	21	laboratory	laboratory	NN
cord-324094-23kzr8rq	51	22	and	and	CC
cord-324094-23kzr8rq	51	23	into	into	IN
cord-324094-23kzr8rq	51	24	the	the	DT
cord-324094-23kzr8rq	51	25	diagnostic	diagnostic	JJ
cord-324094-23kzr8rq	51	26	laboratory	laboratory	NN
cord-324094-23kzr8rq	51	27	(	(	-LRB-
cord-324094-23kzr8rq	51	28	Espy	Espy	NNP
cord-324094-23kzr8rq	51	29	et	et	FW
cord-324094-23kzr8rq	51	30	al	al	NNP
cord-324094-23kzr8rq	51	31	2006	2006	CD
cord-324094-23kzr8rq	51	32	)	)	-RRB-
cord-324094-23kzr8rq	51	33	.	.	.
cord-324094-23kzr8rq	52	1	All	all	DT
cord-324094-23kzr8rq	52	2	real	real	JJ
cord-324094-23kzr8rq	52	3	-	-	HYPH
cord-324094-23kzr8rq	52	4	time	time	NN
cord-324094-23kzr8rq	52	5	PCR	PCR	NNP
cord-324094-23kzr8rq	52	6	systems	system	NNS
cord-324094-23kzr8rq	52	7	rely	rely	VBP
cord-324094-23kzr8rq	52	8	upon	upon	IN
cord-324094-23kzr8rq	52	9	the	the	DT
cord-324094-23kzr8rq	52	10	detection	detection	NN
cord-324094-23kzr8rq	52	11	and	and	CC
cord-324094-23kzr8rq	52	12	quantitation	quantitation	NN
cord-324094-23kzr8rq	52	13	of	of	IN
cord-324094-23kzr8rq	52	14	a	a	DT
cord-324094-23kzr8rq	52	15	fl	fl	NNP
cord-324094-23kzr8rq	52	16	uorescent	uorescent	JJ
cord-324094-23kzr8rq	52	17	reporter	reporter	NN
cord-324094-23kzr8rq	52	18	,	,	,
cord-324094-23kzr8rq	52	19	the	the	DT
cord-324094-23kzr8rq	52	20	signal	signal	NN
cord-324094-23kzr8rq	52	21	of	of	IN
cord-324094-23kzr8rq	52	22	which	which	WDT
cord-324094-23kzr8rq	52	23	increases	increase	NNS
cord-324094-23kzr8rq	52	24	in	in	IN
cord-324094-23kzr8rq	52	25	direct	direct	JJ
cord-324094-23kzr8rq	52	26	proportion	proportion	NN
cord-324094-23kzr8rq	52	27	to	to	IN
cord-324094-23kzr8rq	52	28	the	the	DT
cord-324094-23kzr8rq	52	29	amount	amount	NN
cord-324094-23kzr8rq	52	30	of	of	IN
cord-324094-23kzr8rq	52	31	PCR	PCR	NNP
cord-324094-23kzr8rq	52	32	product	product	NN
cord-324094-23kzr8rq	52	33	in	in	IN
cord-324094-23kzr8rq	52	34	a	a	DT
cord-324094-23kzr8rq	52	35	reaction	reaction	NN
cord-324094-23kzr8rq	52	36	.	.	.
cord-324094-23kzr8rq	53	1	In	in	IN
cord-324094-23kzr8rq	53	2	the	the	DT
cord-324094-23kzr8rq	53	3	simplest	simple	JJS
cord-324094-23kzr8rq	53	4	and	and	CC
cord-324094-23kzr8rq	53	5	most	most	RBS
cord-324094-23kzr8rq	53	6	economical	economical	JJ
cord-324094-23kzr8rq	53	7	format	format	NN
cord-324094-23kzr8rq	53	8	,	,	,
cord-324094-23kzr8rq	53	9	that	that	DT
cord-324094-23kzr8rq	53	10	reporter	reporter	NN
cord-324094-23kzr8rq	53	11	is	be	VBZ
cord-324094-23kzr8rq	53	12	the	the	DT
cord-324094-23kzr8rq	53	13	double	double	JJ
cord-324094-23kzr8rq	53	14	-	-	HYPH
cord-324094-23kzr8rq	53	15	strand	strand	NN
cord-324094-23kzr8rq	53	16	DNA	dna	NN
cord-324094-23kzr8rq	53	17	-	-	HYPH
cord-324094-23kzr8rq	53	18	specifi	specifi	JJ
cord-324094-23kzr8rq	53	19	c	c	NN
cord-324094-23kzr8rq	53	20	dye	dye	NN
cord-324094-23kzr8rq	53	21	SYBR	SYBR	NNP
cord-324094-23kzr8rq	53	22	®	®	.
cord-324094-23kzr8rq	53	23	Green	Green	NNP
cord-324094-23kzr8rq	53	24	(	(	-LRB-
cord-324094-23kzr8rq	53	25	molecular	molecular	JJ
cord-324094-23kzr8rq	53	26	probes	probe	NNS
cord-324094-23kzr8rq	53	27	)	)	-RRB-
cord-324094-23kzr8rq	53	28	.	.	.
cord-324094-23kzr8rq	54	1	SYBR	SYBR	NNP
cord-324094-23kzr8rq	54	2	Green	Green	NNP
cord-324094-23kzr8rq	54	3	binds	bind	VBZ
cord-324094-23kzr8rq	54	4	to	to	IN
cord-324094-23kzr8rq	54	5	double	double	RB
cord-324094-23kzr8rq	54	6	-	-	HYPH
cord-324094-23kzr8rq	54	7	stranded	stranded	JJ
cord-324094-23kzr8rq	54	8	DNA	dna	NN
cord-324094-23kzr8rq	54	9	,	,	,
cord-324094-23kzr8rq	54	10	and	and	CC
cord-324094-23kzr8rq	54	11	upon	upon	IN
cord-324094-23kzr8rq	54	12	excitation	excitation	NN
cord-324094-23kzr8rq	54	13	emits	emit	VBZ
cord-324094-23kzr8rq	54	14	light	light	JJ
cord-324094-23kzr8rq	54	15	.	.	.
cord-324094-23kzr8rq	55	1	Thus	thus	RB
cord-324094-23kzr8rq	55	2	,	,	,
cord-324094-23kzr8rq	55	3	as	as	IN
cord-324094-23kzr8rq	55	4	a	a	DT
cord-324094-23kzr8rq	55	5	PCR	PCR	NNP
cord-324094-23kzr8rq	55	6	product	product	NN
cord-324094-23kzr8rq	55	7	accumulates	accumulate	VBZ
cord-324094-23kzr8rq	55	8	,	,	,
cord-324094-23kzr8rq	55	9	the	the	DT
cord-324094-23kzr8rq	55	10	fl	fl	NNP
cord-324094-23kzr8rq	55	11	uorescence	uorescence	NN
cord-324094-23kzr8rq	55	12	increases	increase	VBZ
cord-324094-23kzr8rq	55	13	.	.	.
cord-324094-23kzr8rq	56	1	The	the	DT
cord-324094-23kzr8rq	56	2	advantages	advantage	NNS
cord-324094-23kzr8rq	56	3	of	of	IN
cord-324094-23kzr8rq	56	4	SYBR	SYBR	NNP
cord-324094-23kzr8rq	56	5	Green	Green	NNP
cord-324094-23kzr8rq	56	6	are	be	VBP
cord-324094-23kzr8rq	56	7	that	that	IN
cord-324094-23kzr8rq	56	8	it	-PRON-	PRP
cord-324094-23kzr8rq	56	9	's	be	VBZ
cord-324094-23kzr8rq	56	10	inexpensive	inexpensive	JJ
cord-324094-23kzr8rq	56	11	,	,	,
cord-324094-23kzr8rq	56	12	easy	easy	JJ
cord-324094-23kzr8rq	56	13	to	to	TO
cord-324094-23kzr8rq	56	14	use	use	VB
cord-324094-23kzr8rq	56	15	,	,	,
cord-324094-23kzr8rq	56	16	and	and	CC
cord-324094-23kzr8rq	56	17	sensitive	sensitive	JJ
cord-324094-23kzr8rq	56	18	.	.	.
cord-324094-23kzr8rq	57	1	The	the	DT
cord-324094-23kzr8rq	57	2	disadvantage	disadvantage	NN
cord-324094-23kzr8rq	57	3	is	be	VBZ
cord-324094-23kzr8rq	57	4	that	that	IN
cord-324094-23kzr8rq	57	5	SYBR	SYBR	NNP
cord-324094-23kzr8rq	57	6	Green	Green	NNP
cord-324094-23kzr8rq	57	7	will	will	MD
cord-324094-23kzr8rq	57	8	bind	bind	VB
cord-324094-23kzr8rq	57	9	to	to	IN
cord-324094-23kzr8rq	57	10	any	any	DT
cord-324094-23kzr8rq	57	11	double	double	RB
cord-324094-23kzr8rq	57	12	-	-	HYPH
cord-324094-23kzr8rq	57	13	stranded	strand	VBN
cord-324094-23kzr8rq	57	14	DNA	dna	NN
cord-324094-23kzr8rq	57	15	in	in	IN
cord-324094-23kzr8rq	57	16	the	the	DT
cord-324094-23kzr8rq	57	17	reaction	reaction	NN
cord-324094-23kzr8rq	57	18	,	,	,
cord-324094-23kzr8rq	57	19	including	include	VBG
cord-324094-23kzr8rq	57	20	primer	primer	NN
cord-324094-23kzr8rq	57	21	-	-	NNS
cord-324094-23kzr8rq	57	22	dimers	dimer	NNS
cord-324094-23kzr8rq	57	23	and	and	CC
cord-324094-23kzr8rq	57	24	other	other	JJ
cord-324094-23kzr8rq	57	25	non	non	JJ
cord-324094-23kzr8rq	57	26	-	-	JJ
cord-324094-23kzr8rq	57	27	specifi	specifi	JJ
cord-324094-23kzr8rq	57	28	c	c	NN
cord-324094-23kzr8rq	57	29	reaction	reaction	NN
cord-324094-23kzr8rq	57	30	products	product	NNS
cord-324094-23kzr8rq	57	31	,	,	,
cord-324094-23kzr8rq	57	32	which	which	WDT
cord-324094-23kzr8rq	57	33	results	result	VBZ
cord-324094-23kzr8rq	57	34	in	in	IN
cord-324094-23kzr8rq	57	35	an	an	DT
cord-324094-23kzr8rq	57	36	overestimation	overestimation	NN
cord-324094-23kzr8rq	57	37	of	of	IN
cord-324094-23kzr8rq	57	38	the	the	DT
cord-324094-23kzr8rq	57	39	target	target	NN
cord-324094-23kzr8rq	57	40	concentration	concentration	NN
cord-324094-23kzr8rq	57	41	.	.	.
cord-324094-23kzr8rq	58	1	For	for	IN
cord-324094-23kzr8rq	58	2	single	single	JJ
cord-324094-23kzr8rq	58	3	PCR	PCR	NNP
cord-324094-23kzr8rq	58	4	product	product	NN
cord-324094-23kzr8rq	58	5	reactions	reaction	NNS
cord-324094-23kzr8rq	58	6	with	with	IN
cord-324094-23kzr8rq	58	7	well	well	RB
cord-324094-23kzr8rq	58	8	-	-	HYPH
cord-324094-23kzr8rq	58	9	designed	design	VBN
cord-324094-23kzr8rq	58	10	primers	primer	NNS
cord-324094-23kzr8rq	58	11	,	,	,
cord-324094-23kzr8rq	58	12	SYBR	SYBR	NNP
cord-324094-23kzr8rq	58	13	Green	Green	NNP
cord-324094-23kzr8rq	58	14	can	can	MD
cord-324094-23kzr8rq	58	15	work	work	VB
cord-324094-23kzr8rq	58	16	extremely	extremely	RB
cord-324094-23kzr8rq	58	17	well	well	RB
cord-324094-23kzr8rq	58	18	,	,	,
cord-324094-23kzr8rq	58	19	with	with	IN
cord-324094-23kzr8rq	58	20	spurious	spurious	JJ
cord-324094-23kzr8rq	58	21	non	non	JJ
cord-324094-23kzr8rq	58	22	-	-	JJ
cord-324094-23kzr8rq	58	23	specifi	specifi	JJ
cord-324094-23kzr8rq	58	24	c	c	NN
cord-324094-23kzr8rq	58	25	background	background	NN
cord-324094-23kzr8rq	58	26	only	only	RB
cord-324094-23kzr8rq	58	27	showing	show	VBG
cord-324094-23kzr8rq	58	28	up	up	RP
cord-324094-23kzr8rq	58	29	in	in	IN
cord-324094-23kzr8rq	58	30	very	very	RB
cord-324094-23kzr8rq	58	31	late	late	JJ
cord-324094-23kzr8rq	58	32	cycles	cycle	NNS
cord-324094-23kzr8rq	58	33	.	.	.
cord-324094-23kzr8rq	59	1	The	the	DT
cord-324094-23kzr8rq	59	2	two	two	CD
cord-324094-23kzr8rq	59	3	most	most	RBS
cord-324094-23kzr8rq	59	4	popular	popular	JJ
cord-324094-23kzr8rq	59	5	alternatives	alternative	NNS
cord-324094-23kzr8rq	59	6	to	to	IN
cord-324094-23kzr8rq	59	7	SYBR	SYBR	NNP
cord-324094-23kzr8rq	59	8	Green	Green	NNP
cord-324094-23kzr8rq	59	9	are	be	VBP
cord-324094-23kzr8rq	59	10	TaqMan	TaqMan	NNP
cord-324094-23kzr8rq	59	11	®	®	,
cord-324094-23kzr8rq	59	12	and	and	CC
cord-324094-23kzr8rq	59	13	molecular	molecular	JJ
cord-324094-23kzr8rq	59	14	beacons	beacon	NNS
cord-324094-23kzr8rq	59	15	,	,	,
cord-324094-23kzr8rq	59	16	both	both	DT
cord-324094-23kzr8rq	59	17	of	of	IN
cord-324094-23kzr8rq	59	18	which	which	WDT
cord-324094-23kzr8rq	59	19	are	be	VBP
cord-324094-23kzr8rq	59	20	hybridization	hybridization	NN
cord-324094-23kzr8rq	59	21	probes	probe	NNS
cord-324094-23kzr8rq	59	22	relying	rely	VBG
cord-324094-23kzr8rq	59	23	on	on	IN
cord-324094-23kzr8rq	59	24	fl	fl	NNP
cord-324094-23kzr8rq	59	25	uorescence	uorescence	NN
cord-324094-23kzr8rq	59	26	resonance	resonance	NN
cord-324094-23kzr8rq	59	27	energy	energy	NN
cord-324094-23kzr8rq	59	28	transfer	transfer	NN
cord-324094-23kzr8rq	59	29	(	(	-LRB-
cord-324094-23kzr8rq	59	30	FRET	fret	NN
cord-324094-23kzr8rq	59	31	)	)	-RRB-
cord-324094-23kzr8rq	59	32	for	for	IN
cord-324094-23kzr8rq	59	33	quantitation	quantitation	NN
cord-324094-23kzr8rq	59	34	(	(	-LRB-
cord-324094-23kzr8rq	59	35	fi	fi	NNP
cord-324094-23kzr8rq	59	36	gure	gure	NNP
cord-324094-23kzr8rq	59	37	1	1	CD
cord-324094-23kzr8rq	59	38	)	)	-RRB-
cord-324094-23kzr8rq	60	1	TaqMan	TaqMan	NNP
cord-324094-23kzr8rq	60	2	Probes	probe	NNS
cord-324094-23kzr8rq	60	3	are	be	VBP
cord-324094-23kzr8rq	60	4	oligonucleotides	oligonucleotide	NNS
cord-324094-23kzr8rq	60	5	that	that	WDT
cord-324094-23kzr8rq	60	6	contain	contain	VBP
cord-324094-23kzr8rq	60	7	a	a	DT
cord-324094-23kzr8rq	60	8	fl	fl	NNP
cord-324094-23kzr8rq	60	9	uorescent	uorescent	JJ
cord-324094-23kzr8rq	60	10	dye	dye	NN
cord-324094-23kzr8rq	60	11	,	,	,
cord-324094-23kzr8rq	60	12	typically	typically	RB
cord-324094-23kzr8rq	60	13	on	on	IN
cord-324094-23kzr8rq	60	14	the	the	DT
cord-324094-23kzr8rq	60	15	5	5	CD
cord-324094-23kzr8rq	60	16	'	'	POS
cord-324094-23kzr8rq	60	17	base	base	NN
cord-324094-23kzr8rq	60	18	,	,	,
cord-324094-23kzr8rq	60	19	and	and	CC
cord-324094-23kzr8rq	60	20	a	a	DT
cord-324094-23kzr8rq	60	21	quenching	quenching	NN
cord-324094-23kzr8rq	60	22	dye	dye	NN
cord-324094-23kzr8rq	60	23	,	,	,
cord-324094-23kzr8rq	60	24	typically	typically	RB
cord-324094-23kzr8rq	60	25	located	locate	VBN
cord-324094-23kzr8rq	60	26	on	on	IN
cord-324094-23kzr8rq	60	27	the	the	DT
cord-324094-23kzr8rq	60	28	3	3	CD
cord-324094-23kzr8rq	60	29	'	'	NN
cord-324094-23kzr8rq	60	30	base	base	NN
cord-324094-23kzr8rq	60	31	.	.	.
cord-324094-23kzr8rq	61	1	When	when	WRB
cord-324094-23kzr8rq	61	2	irradiated	irradiate	VBN
cord-324094-23kzr8rq	61	3	,	,	,
cord-324094-23kzr8rq	61	4	the	the	DT
cord-324094-23kzr8rq	61	5	excited	excited	JJ
cord-324094-23kzr8rq	61	6	fl	fl	NNP
cord-324094-23kzr8rq	61	7	uorescent	uorescent	JJ
cord-324094-23kzr8rq	61	8	dye	dye	NN
cord-324094-23kzr8rq	61	9	transfers	transfer	NNS
cord-324094-23kzr8rq	61	10	energy	energy	NN
cord-324094-23kzr8rq	61	11	to	to	IN
cord-324094-23kzr8rq	61	12	the	the	DT
cord-324094-23kzr8rq	61	13	nearby	nearby	JJ
cord-324094-23kzr8rq	61	14	quenching	quenching	NN
cord-324094-23kzr8rq	61	15	dye	dye	NN
cord-324094-23kzr8rq	61	16	molecule	molecule	NN
cord-324094-23kzr8rq	61	17	rather	rather	RB
cord-324094-23kzr8rq	61	18	than	than	IN
cord-324094-23kzr8rq	61	19	fl	fl	NNP
cord-324094-23kzr8rq	61	20	uorescing	uorescing	NN
cord-324094-23kzr8rq	61	21	,	,	,
cord-324094-23kzr8rq	61	22	resulting	result	VBG
cord-324094-23kzr8rq	61	23	in	in	IN
cord-324094-23kzr8rq	61	24	a	a	DT
cord-324094-23kzr8rq	61	25	nonfl	nonfl	NN
cord-324094-23kzr8rq	61	26	uorescent	uorescent	JJ
cord-324094-23kzr8rq	61	27	substrate	substrate	NN
cord-324094-23kzr8rq	61	28	.	.	.
cord-324094-23kzr8rq	62	1	TaqMan	TaqMan	NNP
cord-324094-23kzr8rq	62	2	probes	probe	NNS
cord-324094-23kzr8rq	62	3	are	be	VBP
cord-324094-23kzr8rq	62	4	designed	design	VBN
cord-324094-23kzr8rq	62	5	to	to	TO
cord-324094-23kzr8rq	62	6	hybridize	hybridize	VB
cord-324094-23kzr8rq	62	7	to	to	IN
cord-324094-23kzr8rq	62	8	an	an	DT
cord-324094-23kzr8rq	62	9	internal	internal	JJ
cord-324094-23kzr8rq	62	10	region	region	NN
cord-324094-23kzr8rq	62	11	of	of	IN
cord-324094-23kzr8rq	62	12	a	a	DT
cord-324094-23kzr8rq	62	13	PCR	PCR	NNP
cord-324094-23kzr8rq	62	14	product	product	NN
cord-324094-23kzr8rq	62	15	.	.	.
cord-324094-23kzr8rq	63	1	During	during	IN
cord-324094-23kzr8rq	63	2	PCR	PCR	NNP
cord-324094-23kzr8rq	63	3	,	,	,
cord-324094-23kzr8rq	63	4	when	when	WRB
cord-324094-23kzr8rq	63	5	the	the	DT
cord-324094-23kzr8rq	63	6	polymerase	polymerase	NN
cord-324094-23kzr8rq	63	7	replicates	replicate	VBZ
cord-324094-23kzr8rq	63	8	a	a	DT
cord-324094-23kzr8rq	63	9	template	template	NN
cord-324094-23kzr8rq	63	10	on	on	IN
cord-324094-23kzr8rq	63	11	which	which	WDT
cord-324094-23kzr8rq	63	12	a	a	DT
cord-324094-23kzr8rq	63	13	TaqMan	TaqMan	NNP
cord-324094-23kzr8rq	63	14	probe	probe	NN
cord-324094-23kzr8rq	63	15	is	be	VBZ
cord-324094-23kzr8rq	63	16	bound	bind	VBN
cord-324094-23kzr8rq	63	17	,	,	,
cord-324094-23kzr8rq	63	18	the	the	DT
cord-324094-23kzr8rq	63	19	5	5	CD
cord-324094-23kzr8rq	63	20	'	'	''
cord-324094-23kzr8rq	63	21	exonuclease	exonuclease	NN
cord-324094-23kzr8rq	63	22	activity	activity	NN
cord-324094-23kzr8rq	63	23	of	of	IN
cord-324094-23kzr8rq	63	24	the	the	DT
cord-324094-23kzr8rq	63	25	polymerase	polymerase	NN
cord-324094-23kzr8rq	63	26	cleaves	cleave	VBZ
cord-324094-23kzr8rq	63	27	the	the	DT
cord-324094-23kzr8rq	63	28	probe	probe	NN
cord-324094-23kzr8rq	63	29	.	.	.
cord-324094-23kzr8rq	64	1	This	this	DT
cord-324094-23kzr8rq	64	2	separates	separate	VBZ
cord-324094-23kzr8rq	64	3	the	the	DT
cord-324094-23kzr8rq	64	4	fl	fl	NNP
cord-324094-23kzr8rq	64	5	uorescent	uorescent	NNP
cord-324094-23kzr8rq	64	6	and	and	CC
cord-324094-23kzr8rq	64	7	quenching	quench	VBG
cord-324094-23kzr8rq	64	8	dyes	dye	NNS
cord-324094-23kzr8rq	64	9	and	and	CC
cord-324094-23kzr8rq	64	10	FRET	fret	VB
cord-324094-23kzr8rq	64	11	no	no	RB
cord-324094-23kzr8rq	64	12	longer	long	RBR
cord-324094-23kzr8rq	64	13	occurs	occur	VBZ
cord-324094-23kzr8rq	64	14	.	.	.
cord-324094-23kzr8rq	65	1	Fluorescence	fluorescence	NN
cord-324094-23kzr8rq	65	2	increases	increase	VBZ
cord-324094-23kzr8rq	65	3	in	in	IN
cord-324094-23kzr8rq	65	4	each	each	DT
cord-324094-23kzr8rq	65	5	cycle	cycle	NN
cord-324094-23kzr8rq	65	6	in	in	IN
cord-324094-23kzr8rq	65	7	proportion	proportion	NN
cord-324094-23kzr8rq	65	8	to	to	IN
cord-324094-23kzr8rq	65	9	the	the	DT
cord-324094-23kzr8rq	65	10	rate	rate	NN
cord-324094-23kzr8rq	65	11	of	of	IN
cord-324094-23kzr8rq	65	12	probe	probe	NN
cord-324094-23kzr8rq	65	13	cleavage	cleavage	NN
cord-324094-23kzr8rq	65	14	fi	fi	NN
cord-324094-23kzr8rq	65	15	gure	gure	NNP
cord-324094-23kzr8rq	65	16	2	2	CD
cord-324094-23kzr8rq	65	17	)	)	-RRB-
cord-324094-23kzr8rq	65	18	.	.	.
cord-324094-23kzr8rq	66	1	Molecular	molecular	JJ
cord-324094-23kzr8rq	66	2	beacons	beacon	NNS
cord-324094-23kzr8rq	66	3	also	also	RB
cord-324094-23kzr8rq	66	4	contain	contain	VBP
cord-324094-23kzr8rq	66	5	fl	fl	NNP
cord-324094-23kzr8rq	66	6	uorescent	uorescent	JJ
cord-324094-23kzr8rq	66	7	and	and	CC
cord-324094-23kzr8rq	66	8	quenching	quench	VBG
cord-324094-23kzr8rq	66	9	dyes	dye	NNS
cord-324094-23kzr8rq	66	10	,	,	,
cord-324094-23kzr8rq	66	11	but	but	CC
cord-324094-23kzr8rq	66	12	FRET	fret	VBP
cord-324094-23kzr8rq	66	13	only	only	RB
cord-324094-23kzr8rq	66	14	occurs	occur	VBZ
cord-324094-23kzr8rq	66	15	when	when	WRB
cord-324094-23kzr8rq	66	16	the	the	DT
cord-324094-23kzr8rq	66	17	quenching	quenching	NN
cord-324094-23kzr8rq	66	18	dye	dye	NN
cord-324094-23kzr8rq	66	19	is	be	VBZ
cord-324094-23kzr8rq	66	20	directly	directly	RB
cord-324094-23kzr8rq	66	21	adjacent	adjacent	JJ
cord-324094-23kzr8rq	66	22	to	to	IN
cord-324094-23kzr8rq	66	23	the	the	DT
cord-324094-23kzr8rq	66	24	fl	fl	NNP
cord-324094-23kzr8rq	66	25	uorescent	uorescent	JJ
cord-324094-23kzr8rq	66	26	dye	dye	NN
cord-324094-23kzr8rq	66	27	.	.	.
cord-324094-23kzr8rq	67	1	Molecular	molecular	JJ
cord-324094-23kzr8rq	67	2	beacons	beacon	NNS
cord-324094-23kzr8rq	67	3	are	be	VBP
cord-324094-23kzr8rq	67	4	designed	design	VBN
cord-324094-23kzr8rq	67	5	to	to	TO
cord-324094-23kzr8rq	67	6	adopt	adopt	VB
cord-324094-23kzr8rq	67	7	a	a	DT
cord-324094-23kzr8rq	67	8	hairpin	hairpin	JJ
cord-324094-23kzr8rq	67	9	structure	structure	NN
cord-324094-23kzr8rq	67	10	while	while	IN
cord-324094-23kzr8rq	67	11	free	free	JJ
cord-324094-23kzr8rq	67	12	in	in	IN
cord-324094-23kzr8rq	67	13	solution	solution	NN
cord-324094-23kzr8rq	67	14	,	,	,
cord-324094-23kzr8rq	67	15	bringing	bring	VBG
cord-324094-23kzr8rq	67	16	the	the	DT
cord-324094-23kzr8rq	67	17	fl	fl	NNP
cord-324094-23kzr8rq	67	18	uorescent	uorescent	JJ
cord-324094-23kzr8rq	67	19	dye	dye	NN
cord-324094-23kzr8rq	67	20	and	and	CC
cord-324094-23kzr8rq	67	21	quencher	quencher	NN
cord-324094-23kzr8rq	67	22	in	in	IN
cord-324094-23kzr8rq	67	23	close	close	JJ
cord-324094-23kzr8rq	67	24	proximity	proximity	NN
cord-324094-23kzr8rq	67	25	.	.	.
cord-324094-23kzr8rq	68	1	When	when	WRB
cord-324094-23kzr8rq	68	2	a	a	DT
cord-324094-23kzr8rq	68	3	molecular	molecular	JJ
cord-324094-23kzr8rq	68	4	beacon	beacon	NN
cord-324094-23kzr8rq	68	5	hybridizes	hybridize	VBZ
cord-324094-23kzr8rq	68	6	to	to	IN
cord-324094-23kzr8rq	68	7	a	a	DT
cord-324094-23kzr8rq	68	8	target	target	NN
cord-324094-23kzr8rq	68	9	,	,	,
cord-324094-23kzr8rq	68	10	the	the	DT
cord-324094-23kzr8rq	68	11	fl	fl	NNP
cord-324094-23kzr8rq	68	12	uorescent	uorescent	JJ
cord-324094-23kzr8rq	68	13	dye	dye	NN
cord-324094-23kzr8rq	68	14	and	and	CC
cord-324094-23kzr8rq	68	15	quenchers	quencher	NNS
cord-324094-23kzr8rq	68	16	are	be	VBP
cord-324094-23kzr8rq	68	17	separated	separate	VBN
cord-324094-23kzr8rq	68	18	,	,	,
cord-324094-23kzr8rq	68	19	FRET	fret	NN
cord-324094-23kzr8rq	68	20	does	do	VBZ
cord-324094-23kzr8rq	68	21	not	not	RB
cord-324094-23kzr8rq	68	22	occur	occur	VB
cord-324094-23kzr8rq	68	23	,	,	,
cord-324094-23kzr8rq	68	24	and	and	CC
cord-324094-23kzr8rq	68	25	the	the	DT
cord-324094-23kzr8rq	68	26	fl	fl	NNP
cord-324094-23kzr8rq	68	27	uorescent	uorescent	JJ
cord-324094-23kzr8rq	68	28	dye	dye	NN
cord-324094-23kzr8rq	68	29	emits	emit	VBZ
cord-324094-23kzr8rq	68	30	light	light	JJ
cord-324094-23kzr8rq	68	31	upon	upon	IN
cord-324094-23kzr8rq	68	32	irradiation	irradiation	NN
cord-324094-23kzr8rq	68	33	.	.	.
cord-324094-23kzr8rq	69	1	Unlike	unlike	IN
cord-324094-23kzr8rq	69	2	TaqMan	TaqMan	NNP
cord-324094-23kzr8rq	69	3	probes	probe	NNS
cord-324094-23kzr8rq	69	4	,	,	,
cord-324094-23kzr8rq	69	5	molecular	molecular	JJ
cord-324094-23kzr8rq	69	6	beacons	beacon	NNS
cord-324094-23kzr8rq	69	7	are	be	VBP
cord-324094-23kzr8rq	69	8	designed	design	VBN
cord-324094-23kzr8rq	69	9	to	to	TO
cord-324094-23kzr8rq	69	10	remain	remain	VB
cord-324094-23kzr8rq	69	11	intact	intact	JJ
cord-324094-23kzr8rq	69	12	during	during	IN
cord-324094-23kzr8rq	69	13	the	the	DT
cord-324094-23kzr8rq	69	14	amplifi	amplifi	NNP
cord-324094-23kzr8rq	69	15	cation	cation	NN
cord-324094-23kzr8rq	69	16	reaction	reaction	NN
cord-324094-23kzr8rq	69	17	,	,	,
cord-324094-23kzr8rq	69	18	and	and	CC
cord-324094-23kzr8rq	69	19	must	must	MD
cord-324094-23kzr8rq	69	20	rebind	rebind	VB
cord-324094-23kzr8rq	69	21	to	to	TO
cord-324094-23kzr8rq	69	22	target	target	VB
cord-324094-23kzr8rq	69	23	in	in	IN
cord-324094-23kzr8rq	69	24	every	every	DT
cord-324094-23kzr8rq	69	25	cycle	cycle	NN
cord-324094-23kzr8rq	69	26	for	for	IN
cord-324094-23kzr8rq	69	27	signal	signal	NN
cord-324094-23kzr8rq	69	28	measurement	measurement	NN
cord-324094-23kzr8rq	69	29	.	.	.
cord-324094-23kzr8rq	70	1	TaqMan	TaqMan	NNP
cord-324094-23kzr8rq	70	2	probes	probe	NNS
cord-324094-23kzr8rq	70	3	and	and	CC
cord-324094-23kzr8rq	70	4	molecular	molecular	JJ
cord-324094-23kzr8rq	70	5	beacons	beacon	NNS
cord-324094-23kzr8rq	70	6	allow	allow	VBP
cord-324094-23kzr8rq	70	7	multiple	multiple	JJ
cord-324094-23kzr8rq	70	8	DNA	dna	NN
cord-324094-23kzr8rq	70	9	species	specie	NNS
cord-324094-23kzr8rq	70	10	to	to	TO
cord-324094-23kzr8rq	70	11	be	be	VB
cord-324094-23kzr8rq	70	12	measured	measure	VBN
cord-324094-23kzr8rq	70	13	in	in	IN
cord-324094-23kzr8rq	70	14	the	the	DT
cord-324094-23kzr8rq	70	15	same	same	JJ
cord-324094-23kzr8rq	70	16	sample	sample	NN
cord-324094-23kzr8rq	70	17	(	(	-LRB-
cord-324094-23kzr8rq	70	18	multiplex	multiplex	JJ
cord-324094-23kzr8rq	70	19	PCR	PCR	NNP
cord-324094-23kzr8rq	70	20	)	)	-RRB-
cord-324094-23kzr8rq	70	21	,	,	,
cord-324094-23kzr8rq	70	22	since	since	IN
cord-324094-23kzr8rq	70	23	fl	fl	NNP
cord-324094-23kzr8rq	70	24	uorescent	uorescent	JJ
cord-324094-23kzr8rq	70	25	dyes	dye	NNS
cord-324094-23kzr8rq	70	26	with	with	IN
cord-324094-23kzr8rq	70	27	different	different	JJ
cord-324094-23kzr8rq	70	28	emission	emission	NN
cord-324094-23kzr8rq	70	29	spectra	spectrum	NNS
cord-324094-23kzr8rq	70	30	may	may	MD
cord-324094-23kzr8rq	70	31	be	be	VB
cord-324094-23kzr8rq	70	32	attached	attach	VBN
cord-324094-23kzr8rq	70	33	to	to	IN
cord-324094-23kzr8rq	70	34	the	the	DT
cord-324094-23kzr8rq	70	35	different	different	JJ
cord-324094-23kzr8rq	70	36	probes	probe	NNS
cord-324094-23kzr8rq	70	37	.	.	.
cord-324094-23kzr8rq	71	1	Multiplex	Multiplex	NNP
cord-324094-23kzr8rq	71	2	PCR	PCR	NNP
cord-324094-23kzr8rq	71	3	allows	allow	VBZ
cord-324094-23kzr8rq	71	4	internal	internal	JJ
cord-324094-23kzr8rq	71	5	controls	control	NNS
cord-324094-23kzr8rq	71	6	to	to	TO
cord-324094-23kzr8rq	71	7	be	be	VB
cord-324094-23kzr8rq	71	8	co	co	VBN
cord-324094-23kzr8rq	71	9	-	-	JJ
cord-324094-23kzr8rq	71	10	amplifi	amplifi	JJ
cord-324094-23kzr8rq	71	11	ed	ed	NN
cord-324094-23kzr8rq	71	12	and	and	CC
cord-324094-23kzr8rq	71	13	permits	permit	VBZ
cord-324094-23kzr8rq	71	14	allele	allele	NN
cord-324094-23kzr8rq	71	15	discrimination	discrimination	NN
cord-324094-23kzr8rq	71	16	in	in	IN
cord-324094-23kzr8rq	71	17	single	single	JJ
cord-324094-23kzr8rq	71	18	-	-	HYPH
cord-324094-23kzr8rq	71	19	tube	tube	NN
cord-324094-23kzr8rq	71	20	,	,	,
cord-324094-23kzr8rq	71	21	homogeneous	homogeneous	JJ
cord-324094-23kzr8rq	71	22	assays	assay	NNS
cord-324094-23kzr8rq	71	23	.	.	.
cord-324094-23kzr8rq	72	1	These	these	DT
cord-324094-23kzr8rq	72	2	hybridization	hybridization	NN
cord-324094-23kzr8rq	72	3	probes	probe	NNS
cord-324094-23kzr8rq	72	4	afford	afford	VBP
cord-324094-23kzr8rq	72	5	a	a	DT
cord-324094-23kzr8rq	72	6	level	level	NN
cord-324094-23kzr8rq	72	7	of	of	IN
cord-324094-23kzr8rq	72	8	discrimination	discrimination	NN
cord-324094-23kzr8rq	72	9	impossible	impossible	JJ
cord-324094-23kzr8rq	72	10	to	to	TO
cord-324094-23kzr8rq	72	11	obtain	obtain	VB
cord-324094-23kzr8rq	72	12	with	with	IN
cord-324094-23kzr8rq	72	13	SYBR	SYBR	NNP
cord-324094-23kzr8rq	72	14	Green	Green	NNP
cord-324094-23kzr8rq	72	15	,	,	,
cord-324094-23kzr8rq	72	16	since	since	IN
cord-324094-23kzr8rq	72	17	they	-PRON-	PRP
cord-324094-23kzr8rq	72	18	will	will	MD
cord-324094-23kzr8rq	72	19	only	only	RB
cord-324094-23kzr8rq	72	20	hybridize	hybridize	VB
cord-324094-23kzr8rq	72	21	to	to	IN
cord-324094-23kzr8rq	72	22	true	true	JJ
cord-324094-23kzr8rq	72	23	targets	target	NNS
cord-324094-23kzr8rq	72	24	in	in	IN
cord-324094-23kzr8rq	72	25	a	a	DT
cord-324094-23kzr8rq	72	26	PCR	pcr	NN
cord-324094-23kzr8rq	72	27	and	and	CC
cord-324094-23kzr8rq	72	28	not	not	RB
cord-324094-23kzr8rq	72	29	to	to	TO
cord-324094-23kzr8rq	72	30	primer	primer	VB
cord-324094-23kzr8rq	72	31	-	-	NNS
cord-324094-23kzr8rq	72	32	dimers	dimer	NNS
cord-324094-23kzr8rq	72	33	or	or	CC
cord-324094-23kzr8rq	72	34	other	other	JJ
cord-324094-23kzr8rq	72	35	spurious	spurious	JJ
cord-324094-23kzr8rq	72	36	products	product	NNS
cord-324094-23kzr8rq	72	37	.	.	.
cord-324094-23kzr8rq	73	1	RT	RT	NNP
cord-324094-23kzr8rq	73	2	-	-	HYPH
cord-324094-23kzr8rq	73	3	PCR	PCR	NNP
cord-324094-23kzr8rq	73	4	has	have	VBZ
cord-324094-23kzr8rq	73	5	become	become	VBN
cord-324094-23kzr8rq	73	6	the	the	DT
cord-324094-23kzr8rq	73	7	benchmark	benchmark	NN
cord-324094-23kzr8rq	73	8	for	for	IN
cord-324094-23kzr8rq	73	9	the	the	DT
cord-324094-23kzr8rq	73	10	detection	detection	NN
cord-324094-23kzr8rq	73	11	and	and	CC
cord-324094-23kzr8rq	73	12	quantifi	quantifi	NNP
cord-324094-23kzr8rq	73	13	cation	cation	NN
cord-324094-23kzr8rq	73	14	of	of	IN
cord-324094-23kzr8rq	73	15	viruses	virus	NNS
cord-324094-23kzr8rq	73	16	and	and	CC
cord-324094-23kzr8rq	73	17	is	be	VBZ
cord-324094-23kzr8rq	73	18	being	be	VBG
cord-324094-23kzr8rq	73	19	utilized	utilize	VBN
cord-324094-23kzr8rq	73	20	increasingly	increasingly	RB
cord-324094-23kzr8rq	73	21	in	in	IN
cord-324094-23kzr8rq	73	22	novel	novel	JJ
cord-324094-23kzr8rq	73	23	clinical	clinical	JJ
cord-324094-23kzr8rq	73	24	diagnostic	diagnostic	JJ
cord-324094-23kzr8rq	73	25	assays	assay	NNS
cord-324094-23kzr8rq	73	26	.	.	.
cord-324094-23kzr8rq	74	1	Quantitative	quantitative	JJ
cord-324094-23kzr8rq	74	2	results	result	NNS
cord-324094-23kzr8rq	74	3	obtained	obtain	VBN
cord-324094-23kzr8rq	74	4	by	by	IN
cord-324094-23kzr8rq	74	5	this	this	DT
cord-324094-23kzr8rq	74	6	technology	technology	NN
cord-324094-23kzr8rq	74	7	are	be	VBP
cord-324094-23kzr8rq	74	8	not	not	RB
cord-324094-23kzr8rq	74	9	only	only	RB
cord-324094-23kzr8rq	74	10	more	more	RBR
cord-324094-23kzr8rq	74	11	informative	informative	JJ
cord-324094-23kzr8rq	74	12	than	than	IN
cord-324094-23kzr8rq	74	13	qualitative	qualitative	JJ
cord-324094-23kzr8rq	74	14	data	datum	NNS
cord-324094-23kzr8rq	74	15	,	,	,
cord-324094-23kzr8rq	74	16	but	but	CC
cord-324094-23kzr8rq	74	17	simplify	simplify	VB
cord-324094-23kzr8rq	74	18	assay	assay	NN
cord-324094-23kzr8rq	74	19	standardization	standardization	NN
cord-324094-23kzr8rq	74	20	and	and	CC
cord-324094-23kzr8rq	74	21	quality	quality	NN
cord-324094-23kzr8rq	74	22	management	management	NN
cord-324094-23kzr8rq	74	23	.	.	.
cord-324094-23kzr8rq	75	1	Quantitative	quantitative	JJ
cord-324094-23kzr8rq	75	2	PCR	PCR	NNP
cord-324094-23kzr8rq	75	3	(	(	-LRB-
cord-324094-23kzr8rq	75	4	QPCR	QPCR	NNP
cord-324094-23kzr8rq	75	5	)	)	-RRB-
cord-324094-23kzr8rq	76	1	assays	assay	NNS
cord-324094-23kzr8rq	76	2	are	be	VBP
cord-324094-23kzr8rq	76	3	most	most	RBS
cord-324094-23kzr8rq	76	4	established	establish	VBN
cord-324094-23kzr8rq	76	5	for	for	IN
cord-324094-23kzr8rq	76	6	the	the	DT
cord-324094-23kzr8rq	76	7	detection	detection	NN
cord-324094-23kzr8rq	76	8	of	of	IN
cord-324094-23kzr8rq	76	9	viral	viral	JJ
cord-324094-23kzr8rq	76	10	load	load	NN
cord-324094-23kzr8rq	76	11	and	and	CC
cord-324094-23kzr8rq	76	12	therapy	therapy	NN
cord-324094-23kzr8rq	76	13	monitoring	monitoring	NN
cord-324094-23kzr8rq	76	14	(	(	-LRB-
cord-324094-23kzr8rq	76	15	Mackay	Mackay	NNP
cord-324094-23kzr8rq	76	16	et	et	NNP
cord-324094-23kzr8rq	76	17	al	al	NNP
cord-324094-23kzr8rq	76	18	2002	2002	CD
cord-324094-23kzr8rq	76	19	)	)	-RRB-
cord-324094-23kzr8rq	76	20	.	.	.
cord-324094-23kzr8rq	77	1	Further	further	RB
cord-324094-23kzr8rq	77	2	,	,	,
cord-324094-23kzr8rq	77	3	nucleotide	nucleotide	JJ
cord-324094-23kzr8rq	77	4	sequence	sequence	NN
cord-324094-23kzr8rq	77	5	analysis	analysis	NN
cord-324094-23kzr8rq	77	6	of	of	IN
cord-324094-23kzr8rq	77	7	the	the	DT
cord-324094-23kzr8rq	77	8	amplifi	amplifi	NNP
cord-324094-23kzr8rq	77	9	cation	cation	NN
cord-324094-23kzr8rq	77	10	products	product	NNS
cord-324094-23kzr8rq	77	11	has	have	VBZ
cord-324094-23kzr8rq	77	12	facilitated	facilitate	VBN
cord-324094-23kzr8rq	77	13	epidemiological	epidemiological	JJ
cord-324094-23kzr8rq	77	14	studies	study	NNS
cord-324094-23kzr8rq	77	15	of	of	IN
cord-324094-23kzr8rq	77	16	infectious	infectious	JJ
cord-324094-23kzr8rq	77	17	disease	disease	NN
cord-324094-23kzr8rq	77	18	outbreaks	outbreak	NNS
cord-324094-23kzr8rq	77	19	,	,	,
cord-324094-23kzr8rq	77	20	and	and	CC
cord-324094-23kzr8rq	77	21	the	the	DT
cord-324094-23kzr8rq	77	22	monitoring	monitoring	NN
cord-324094-23kzr8rq	77	23	of	of	IN
cord-324094-23kzr8rq	77	24	treatment	treatment	NN
cord-324094-23kzr8rq	77	25	outcomes	outcome	NNS
cord-324094-23kzr8rq	77	26	for	for	IN
cord-324094-23kzr8rq	77	27	infections	infection	NNS
cord-324094-23kzr8rq	77	28	,	,	,
cord-324094-23kzr8rq	77	29	in	in	IN
cord-324094-23kzr8rq	77	30	particular	particular	JJ
cord-324094-23kzr8rq	77	31	with	with	IN
cord-324094-23kzr8rq	77	32	viruses	virus	NNS
cord-324094-23kzr8rq	77	33	which	which	WDT
cord-324094-23kzr8rq	77	34	mutate	mutate	VBP
cord-324094-23kzr8rq	77	35	at	at	IN
cord-324094-23kzr8rq	77	36	high	high	JJ
cord-324094-23kzr8rq	77	37	frequency	frequency	NN
cord-324094-23kzr8rq	77	38	.	.	.
cord-324094-23kzr8rq	78	1	Several	several	JJ
cord-324094-23kzr8rq	78	2	investigators	investigator	NNS
cord-324094-23kzr8rq	78	3	have	have	VBP
cord-324094-23kzr8rq	78	4	reported	report	VBN
cord-324094-23kzr8rq	78	5	fully	fully	RB
cord-324094-23kzr8rq	78	6	automatic	automatic	JJ
cord-324094-23kzr8rq	78	7	realtime	realtime	NN
cord-324094-23kzr8rq	78	8	PCR	pcr	NN
cord-324094-23kzr8rq	78	9	assays	assay	NNS
cord-324094-23kzr8rq	78	10	for	for	IN
cord-324094-23kzr8rq	78	11	the	the	DT
cord-324094-23kzr8rq	78	12	detection	detection	NN
cord-324094-23kzr8rq	78	13	of	of	IN
cord-324094-23kzr8rq	78	14	viruses	virus	NNS
cord-324094-23kzr8rq	78	15	in	in	IN
cord-324094-23kzr8rq	78	16	acute	acute	JJ
cord-324094-23kzr8rq	78	17	-	-	HYPH
cord-324094-23kzr8rq	78	18	phase	phase	NN
cord-324094-23kzr8rq	78	19	serum	serum	NN
cord-324094-23kzr8rq	78	20	samples	sample	NNS
cord-324094-23kzr8rq	78	21	.	.	.
cord-324094-23kzr8rq	79	1	With	with	IN
cord-324094-23kzr8rq	79	2	improved	improve	VBN
cord-324094-23kzr8rq	79	3	and	and	CC
cord-324094-23kzr8rq	79	4	automated	automate	VBN
cord-324094-23kzr8rq	79	5	nucleic	nucleic	JJ
cord-324094-23kzr8rq	79	6	acid	acid	NN
cord-324094-23kzr8rq	79	7	sample	sample	NN
cord-324094-23kzr8rq	79	8	isolation	isolation	NN
cord-324094-23kzr8rq	79	9	techniques	technique	NNS
cord-324094-23kzr8rq	79	10	,	,	,
cord-324094-23kzr8rq	79	11	as	as	RB
cord-324094-23kzr8rq	79	12	well	well	RB
cord-324094-23kzr8rq	79	13	as	as	IN
cord-324094-23kzr8rq	79	14	real	real	JJ
cord-324094-23kzr8rq	79	15	-	-	HYPH
cord-324094-23kzr8rq	79	16	time	time	NN
cord-324094-23kzr8rq	79	17	detection	detection	NN
cord-324094-23kzr8rq	79	18	methods	method	NNS
cord-324094-23kzr8rq	79	19	,	,	,
cord-324094-23kzr8rq	79	20	a	a	DT
cord-324094-23kzr8rq	79	21	new	new	JJ
cord-324094-23kzr8rq	79	22	generation	generation	NN
cord-324094-23kzr8rq	79	23	of	of	IN
cord-324094-23kzr8rq	79	24	assays	assay	NNS
cord-324094-23kzr8rq	79	25	for	for	IN
cord-324094-23kzr8rq	79	26	most	most	RBS
cord-324094-23kzr8rq	79	27	clinically	clinically	RB
cord-324094-23kzr8rq	79	28	important	important	JJ
cord-324094-23kzr8rq	79	29	viruses	virus	NNS
cord-324094-23kzr8rq	79	30	is	be	VBZ
cord-324094-23kzr8rq	79	31	being	be	VBG
cord-324094-23kzr8rq	79	32	developed	develop	VBN
cord-324094-23kzr8rq	79	33	(	(	-LRB-
cord-324094-23kzr8rq	79	34	Niesters	Niesters	NNP
cord-324094-23kzr8rq	79	35	2002	2002	CD
cord-324094-23kzr8rq	79	36	)	)	-RRB-
cord-324094-23kzr8rq	79	37	.	.	.
cord-324094-23kzr8rq	80	1	QPCR	QPCR	NNP
cord-324094-23kzr8rq	80	2	assays	assay	NNS
cord-324094-23kzr8rq	80	3	based	base	VBN
cord-324094-23kzr8rq	80	4	on	on	IN
cord-324094-23kzr8rq	80	5	SYBR	SYBR	NNP
cord-324094-23kzr8rq	80	6	Green	Green	NNP
cord-324094-23kzr8rq	80	7	and	and	CC
cord-324094-23kzr8rq	80	8	TaqMan	TaqMan	NNP
cord-324094-23kzr8rq	80	9	chemistries	chemistry	NNS
cord-324094-23kzr8rq	80	10	have	have	VBP
cord-324094-23kzr8rq	80	11	been	be	VBN
cord-324094-23kzr8rq	80	12	developed	develop	VBN
cord-324094-23kzr8rq	80	13	and	and	CC
cord-324094-23kzr8rq	80	14	validated	validate	VBN
cord-324094-23kzr8rq	80	15	and	and	CC
cord-324094-23kzr8rq	80	16	are	be	VBP
cord-324094-23kzr8rq	80	17	beginning	begin	VBG
cord-324094-23kzr8rq	80	18	to	to	TO
cord-324094-23kzr8rq	80	19	reveal	reveal	VB
cord-324094-23kzr8rq	80	20	the	the	DT
cord-324094-23kzr8rq	80	21	virus	virus	NN
cord-324094-23kzr8rq	80	22	's	's	POS
cord-324094-23kzr8rq	80	23	epidemiology	epidemiology	NN
cord-324094-23kzr8rq	80	24	and	and	CC
cord-324094-23kzr8rq	80	25	pathogenesis	pathogenesis	NN
cord-324094-23kzr8rq	81	1	(	(	-LRB-
cord-324094-23kzr8rq	81	2	Gunson	Gunson	NNP
cord-324094-23kzr8rq	81	3	et	et	NNP
cord-324094-23kzr8rq	81	4	al	al	NNP
cord-324094-23kzr8rq	81	5	2006	2006	CD
cord-324094-23kzr8rq	81	6	;	;	:
cord-324094-23kzr8rq	81	7	Watzinger	Watzinger	NNP
cord-324094-23kzr8rq	81	8	et	et	NNP
cord-324094-23kzr8rq	81	9	al	al	NNP
cord-324094-23kzr8rq	81	10	2006	2006	CD
cord-324094-23kzr8rq	81	11	)	)	-RRB-
cord-324094-23kzr8rq	81	12	.	.	.
cord-324094-23kzr8rq	82	1	The	the	DT
cord-324094-23kzr8rq	82	2	QPCR	QPCR	NNP
cord-324094-23kzr8rq	82	3	assay	assay	NN
cord-324094-23kzr8rq	82	4	also	also	RB
cord-324094-23kzr8rq	82	5	provides	provide	VBZ
cord-324094-23kzr8rq	82	6	critical	critical	JJ
cord-324094-23kzr8rq	82	7	prognostic	prognostic	JJ
cord-324094-23kzr8rq	82	8	information	information	NN
cord-324094-23kzr8rq	82	9	for	for	IN
cord-324094-23kzr8rq	82	10	clinical	clinical	JJ
cord-324094-23kzr8rq	82	11	management	management	NN
cord-324094-23kzr8rq	82	12	.	.	.
cord-324094-23kzr8rq	83	1	More	more	RBR
cord-324094-23kzr8rq	83	2	recently	recently	RB
cord-324094-23kzr8rq	83	3	,	,	,
cord-324094-23kzr8rq	83	4	real	real	JJ
cord-324094-23kzr8rq	83	5	-	-	HYPH
cord-324094-23kzr8rq	83	6	time	time	NN
cord-324094-23kzr8rq	83	7	PCR	PCR	NNP
cord-324094-23kzr8rq	83	8	assays	assay	NNS
cord-324094-23kzr8rq	83	9	have	have	VBP
cord-324094-23kzr8rq	83	10	provided	provide	VBN
cord-324094-23kzr8rq	83	11	additional	additional	JJ
cord-324094-23kzr8rq	83	12	major	major	JJ
cord-324094-23kzr8rq	83	13	contributions	contribution	NNS
cord-324094-23kzr8rq	83	14	,	,	,
cord-324094-23kzr8rq	83	15	with	with	IN
cord-324094-23kzr8rq	83	16	the	the	DT
cord-324094-23kzr8rq	83	17	inclusion	inclusion	NN
cord-324094-23kzr8rq	83	18	of	of	IN
cord-324094-23kzr8rq	83	19	an	an	DT
cord-324094-23kzr8rq	83	20	additional	additional	JJ
cord-324094-23kzr8rq	83	21	fl	fl	NNP
cord-324094-23kzr8rq	83	22	uorescent	uorescent	NNP
cord-324094-23kzr8rq	83	23	probe	probe	NN
cord-324094-23kzr8rq	83	24	detection	detection	NN
cord-324094-23kzr8rq	83	25	system	system	NN
cord-324094-23kzr8rq	83	26	resulting	result	VBG
cord-324094-23kzr8rq	83	27	in	in	IN
cord-324094-23kzr8rq	83	28	an	an	DT
cord-324094-23kzr8rq	83	29	increase	increase	NN
cord-324094-23kzr8rq	83	30	in	in	IN
cord-324094-23kzr8rq	83	31	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	83	32	over	over	IN
cord-324094-23kzr8rq	83	33	conventional	conventional	JJ
cord-324094-23kzr8rq	83	34	PCR	PCR	NNP
cord-324094-23kzr8rq	83	35	,	,	,
cord-324094-23kzr8rq	83	36	the	the	DT
cord-324094-23kzr8rq	83	37	ability	ability	NN
cord-324094-23kzr8rq	83	38	to	to	TO
cord-324094-23kzr8rq	83	39	confi	confi	NNP
cord-324094-23kzr8rq	83	40	rm	rm	NNP
cord-324094-23kzr8rq	83	41	the	the	DT
cord-324094-23kzr8rq	83	42	amplifi	amplifi	NNP
cord-324094-23kzr8rq	83	43	cation	cation	NN
cord-324094-23kzr8rq	83	44	product	product	NN
cord-324094-23kzr8rq	83	45	and	and	CC
cord-324094-23kzr8rq	83	46	to	to	TO
cord-324094-23kzr8rq	83	47	quantitate	quantitate	VB
cord-324094-23kzr8rq	83	48	the	the	DT
cord-324094-23kzr8rq	83	49	target	target	NN
cord-324094-23kzr8rq	83	50	concentration	concentration	NN
cord-324094-23kzr8rq	83	51	and	and	CC
cord-324094-23kzr8rq	83	52	detection	detection	NN
cord-324094-23kzr8rq	83	53	of	of	IN
cord-324094-23kzr8rq	83	54	multiple	multiple	JJ
cord-324094-23kzr8rq	83	55	targets	target	NNS
cord-324094-23kzr8rq	83	56	in	in	IN
cord-324094-23kzr8rq	83	57	a	a	DT
cord-324094-23kzr8rq	83	58	single	single	JJ
cord-324094-23kzr8rq	83	59	tube	tube	NN
cord-324094-23kzr8rq	83	60	.	.	.
cord-324094-23kzr8rq	84	1	Multiplex	multiplex	JJ
cord-324094-23kzr8rq	84	2	real	real	JJ
cord-324094-23kzr8rq	84	3	-	-	HYPH
cord-324094-23kzr8rq	84	4	time	time	NN
cord-324094-23kzr8rq	84	5	quantitative	quantitative	JJ
cord-324094-23kzr8rq	84	6	RT	RT	NNP
cord-324094-23kzr8rq	84	7	-	-	HYPH
cord-324094-23kzr8rq	84	8	PCR	PCR	NNP
cord-324094-23kzr8rq	84	9	assays	assay	NNS
cord-324094-23kzr8rq	84	10	have	have	VBP
cord-324094-23kzr8rq	84	11	been	be	VBN
cord-324094-23kzr8rq	84	12	developed	develop	VBN
cord-324094-23kzr8rq	84	13	for	for	IN
cord-324094-23kzr8rq	84	14	simultaneous	simultaneous	JJ
cord-324094-23kzr8rq	84	15	detection	detection	NN
cord-324094-23kzr8rq	84	16	,	,	,
cord-324094-23kzr8rq	84	17	identifi	identifi	NNP
cord-324094-23kzr8rq	84	18	cation	cation	NNP
cord-324094-23kzr8rq	84	19	and	and	CC
cord-324094-23kzr8rq	84	20	quantifi	quantifi	NNP
cord-324094-23kzr8rq	84	21	cation	cation	NN
cord-324094-23kzr8rq	84	22	of	of	IN
cord-324094-23kzr8rq	84	23	HBV	HBV	NNP
cord-324094-23kzr8rq	84	24	,	,	,
cord-324094-23kzr8rq	84	25	HCV	HCV	NNP
cord-324094-23kzr8rq	84	26	and	and	CC
cord-324094-23kzr8rq	84	27	HIV-1	HIV-1	NNP
cord-324094-23kzr8rq	84	28	in	in	IN
cord-324094-23kzr8rq	84	29	plasma	plasma	NN
cord-324094-23kzr8rq	84	30	or	or	CC
cord-324094-23kzr8rq	84	31	serum	serum	NN
cord-324094-23kzr8rq	84	32	samples	sample	NNS
cord-324094-23kzr8rq	84	33	.	.	.
cord-324094-23kzr8rq	85	1	Genomic	genomic	JJ
cord-324094-23kzr8rq	85	2	amplifi	amplifi	NNP
cord-324094-23kzr8rq	85	3	cation	cation	NN
cord-324094-23kzr8rq	85	4	of	of	IN
cord-324094-23kzr8rq	85	5	one	one	CD
cord-324094-23kzr8rq	85	6	virus	virus	NN
cord-324094-23kzr8rq	86	1	was	be	VBD
cord-324094-23kzr8rq	86	2	unaffected	unaffected	JJ
cord-324094-23kzr8rq	86	3	by	by	IN
cord-324094-23kzr8rq	86	4	the	the	DT
cord-324094-23kzr8rq	86	5	simultaneous	simultaneous	JJ
cord-324094-23kzr8rq	86	6	amplifi	amplifi	NNP
cord-324094-23kzr8rq	86	7	cation	cation	NN
cord-324094-23kzr8rq	86	8	of	of	IN
cord-324094-23kzr8rq	86	9	the	the	DT
cord-324094-23kzr8rq	86	10	other	other	JJ
cord-324094-23kzr8rq	86	11	two	two	CD
cord-324094-23kzr8rq	86	12	.	.	.
cord-324094-23kzr8rq	87	1	Although	although	IN
cord-324094-23kzr8rq	87	2	competition	competition	NN
cord-324094-23kzr8rq	87	3	between	between	IN
cord-324094-23kzr8rq	87	4	HCV	HCV	NNP
cord-324094-23kzr8rq	87	5	and	and	CC
cord-324094-23kzr8rq	87	6	HIV-1	HIV-1	NNP
cord-324094-23kzr8rq	87	7	amplifi	amplifi	VBP
cord-324094-23kzr8rq	87	8	cations	cation	NNS
cord-324094-23kzr8rq	87	9	slightly	slightly	RB
cord-324094-23kzr8rq	87	10	affected	affect	VBD
cord-324094-23kzr8rq	87	11	the	the	DT
cord-324094-23kzr8rq	87	12	yield	yield	NN
cord-324094-23kzr8rq	87	13	of	of	IN
cord-324094-23kzr8rq	87	14	HIV-1	HIV-1	NNP
cord-324094-23kzr8rq	87	15	amplifi	amplifi	NNP
cord-324094-23kzr8rq	87	16	cation	cation	NN
cord-324094-23kzr8rq	87	17	,	,	,
cord-324094-23kzr8rq	87	18	quantifi	quantifi	NNP
cord-324094-23kzr8rq	87	19	cation	cation	NN
cord-324094-23kzr8rq	87	20	of	of	IN
cord-324094-23kzr8rq	87	21	a	a	DT
cord-324094-23kzr8rq	87	22	single	single	JJ
cord-324094-23kzr8rq	87	23	virus	virus	NN
cord-324094-23kzr8rq	87	24	was	be	VBD
cord-324094-23kzr8rq	87	25	possible	possible	JJ
cord-324094-23kzr8rq	87	26	.	.	.
cord-324094-23kzr8rq	88	1	A	a	DT
cord-324094-23kzr8rq	88	2	novel	novel	JJ
cord-324094-23kzr8rq	88	3	QRTPCR	QRTPCR	NNP
cord-324094-23kzr8rq	88	4	assay	assay	NN
cord-324094-23kzr8rq	88	5	consists	consist	VBZ
cord-324094-23kzr8rq	88	6	of	of	IN
cord-324094-23kzr8rq	88	7	two	two	CD
cord-324094-23kzr8rq	88	8	multiplex	multiplex	JJ
cord-324094-23kzr8rq	88	9	reactions	reaction	NNS
cord-324094-23kzr8rq	88	10	,	,	,
cord-324094-23kzr8rq	88	11	one	one	CD
cord-324094-23kzr8rq	88	12	detecting	detect	VBG
cord-324094-23kzr8rq	88	13	infl	infl	NN
cord-324094-23kzr8rq	88	14	uenza	uenza	NNP
cord-324094-23kzr8rq	88	15	A	A	NNP
cord-324094-23kzr8rq	88	16	and	and	CC
cord-324094-23kzr8rq	88	17	B	B	NNP
cord-324094-23kzr8rq	88	18	and	and	CC
cord-324094-23kzr8rq	88	19	RSV	RSV	NNP
cord-324094-23kzr8rq	88	20	,	,	,
cord-324094-23kzr8rq	88	21	the	the	DT
cord-324094-23kzr8rq	88	22	other	other	JJ
cord-324094-23kzr8rq	88	23	one	one	CD
cord-324094-23kzr8rq	88	24	PIV	PIV	NNP
cord-324094-23kzr8rq	88	25	1	1	CD
cord-324094-23kzr8rq	88	26	-	-	SYM
cord-324094-23kzr8rq	88	27	4	4	CD
cord-324094-23kzr8rq	88	28	,	,	,
cord-324094-23kzr8rq	88	29	and	and	CC
cord-324094-23kzr8rq	88	30	can	can	MD
cord-324094-23kzr8rq	88	31	generate	generate	VB
cord-324094-23kzr8rq	88	32	a	a	DT
cord-324094-23kzr8rq	88	33	result	result	NN
cord-324094-23kzr8rq	88	34	within	within	IN
cord-324094-23kzr8rq	88	35	6	6	CD
cord-324094-23kzr8rq	88	36	h.	h.	NN
cord-324094-23kzr8rq	88	37	Other	other	JJ
cord-324094-23kzr8rq	88	38	real	real	JJ
cord-324094-23kzr8rq	88	39	-	-	HYPH
cord-324094-23kzr8rq	88	40	time	time	NN
cord-324094-23kzr8rq	88	41	assays	assay	NNS
cord-324094-23kzr8rq	88	42	can	can	MD
cord-324094-23kzr8rq	88	43	differentiate	differentiate	VB
cord-324094-23kzr8rq	88	44	between	between	IN
cord-324094-23kzr8rq	88	45	infl	infl	NN
cord-324094-23kzr8rq	88	46	uenza	uenza	NNP
cord-324094-23kzr8rq	88	47	A	a	DT
cord-324094-23kzr8rq	88	48	subtypes	subtype	NNS
cord-324094-23kzr8rq	88	49	and	and	CC
cord-324094-23kzr8rq	88	50	RSV	RSV	NNP
cord-324094-23kzr8rq	88	51	A	A	NNP
cord-324094-23kzr8rq	88	52	and	and	CC
cord-324094-23kzr8rq	88	53	B	b	NN
cord-324094-23kzr8rq	88	54	facilitating	facilitate	VBG
cord-324094-23kzr8rq	88	55	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	88	56	,	,	,
cord-324094-23kzr8rq	88	57	patient	patient	JJ
cord-324094-23kzr8rq	88	58	management	management	NN
cord-324094-23kzr8rq	88	59	and	and	CC
cord-324094-23kzr8rq	88	60	strain	strain	VB
cord-324094-23kzr8rq	88	61	identifi	identifi	NNP
cord-324094-23kzr8rq	88	62	cation	cation	NNP
cord-324094-23kzr8rq	88	63	for	for	IN
cord-324094-23kzr8rq	88	64	vaccine	vaccine	NN
cord-324094-23kzr8rq	88	65	production	production	NN
cord-324094-23kzr8rq	88	66	.	.	.
cord-324094-23kzr8rq	89	1	QRTPCR	QRTPCR	NNP
cord-324094-23kzr8rq	89	2	based	base	VBD
cord-324094-23kzr8rq	89	3	diagnostic	diagnostic	JJ
cord-324094-23kzr8rq	89	4	assays	assay	NNS
cord-324094-23kzr8rq	89	5	have	have	VBP
cord-324094-23kzr8rq	89	6	also	also	RB
cord-324094-23kzr8rq	89	7	been	be	VBN
cord-324094-23kzr8rq	89	8	reported	report	VBN
cord-324094-23kzr8rq	89	9	for	for	IN
cord-324094-23kzr8rq	89	10	the	the	DT
cord-324094-23kzr8rq	89	11	associated	associated	NNP
cord-324094-23kzr8rq	89	12	corona	corona	NN
cord-324094-23kzr8rq	89	13	viruses	virus	NNS
cord-324094-23kzr8rq	89	14	and	and	CC
cord-324094-23kzr8rq	89	15	improved	improve	VBD
cord-324094-23kzr8rq	89	16	to	to	TO
cord-324094-23kzr8rq	89	17	give	give	VB
cord-324094-23kzr8rq	89	18	result	result	NN
cord-324094-23kzr8rq	89	19	in	in	IN
cord-324094-23kzr8rq	89	20	an	an	DT
cord-324094-23kzr8rq	89	21	assay	assay	NN
cord-324094-23kzr8rq	89	22	with	with	IN
cord-324094-23kzr8rq	89	23	100	100	CD
cord-324094-23kzr8rq	89	24	%	%	NN
cord-324094-23kzr8rq	89	25	specifi	specifi	NN
cord-324094-23kzr8rq	89	26	city	city	NN
cord-324094-23kzr8rq	89	27	in	in	IN
cord-324094-23kzr8rq	89	28	samples	sample	NNS
cord-324094-23kzr8rq	89	29	collected	collect	VBN
cord-324094-23kzr8rq	89	30	from	from	IN
cord-324094-23kzr8rq	89	31	day	day	NN
cord-324094-23kzr8rq	89	32	1	1	CD
cord-324094-23kzr8rq	89	33	-	-	SYM
cord-324094-23kzr8rq	89	34	3	3	CD
cord-324094-23kzr8rq	89	35	of	of	IN
cord-324094-23kzr8rq	89	36	disease	disease	NN
cord-324094-23kzr8rq	89	37	onset	onset	NN
cord-324094-23kzr8rq	89	38	.	.	.
cord-324094-23kzr8rq	90	1	The	the	DT
cord-324094-23kzr8rq	90	2	assay	assay	NN
cord-324094-23kzr8rq	90	3	's	's	POS
cord-324094-23kzr8rq	90	4	potential	potential	NN
cord-324094-23kzr8rq	90	5	for	for	IN
cord-324094-23kzr8rq	90	6	high	high	JJ
cord-324094-23kzr8rq	90	7	throughput	throughput	NN
cord-324094-23kzr8rq	90	8	was	be	VBD
cord-324094-23kzr8rq	90	9	also	also	RB
cord-324094-23kzr8rq	90	10	of	of	IN
cord-324094-23kzr8rq	90	11	critical	critical	JJ
cord-324094-23kzr8rq	90	12	importance	importance	NN
cord-324094-23kzr8rq	90	13	in	in	IN
cord-324094-23kzr8rq	90	14	areas	area	NNS
cord-324094-23kzr8rq	90	15	with	with	IN
cord-324094-23kzr8rq	90	16	outbreaks	outbreak	NNS
cord-324094-23kzr8rq	90	17	of	of	IN
cord-324094-23kzr8rq	90	18	SARS	SARS	NNP
cord-324094-23kzr8rq	90	19	in	in	IN
cord-324094-23kzr8rq	90	20	which	which	WDT
cord-324094-23kzr8rq	90	21	large	large	JJ
cord-324094-23kzr8rq	90	22	numbers	number	NNS
cord-324094-23kzr8rq	90	23	of	of	IN
cord-324094-23kzr8rq	90	24	specimens	specimen	NNS
cord-324094-23kzr8rq	90	25	had	have	VBD
cord-324094-23kzr8rq	90	26	to	to	TO
cord-324094-23kzr8rq	90	27	be	be	VB
cord-324094-23kzr8rq	90	28	tested	test	VBN
cord-324094-23kzr8rq	90	29	.	.	.
cord-324094-23kzr8rq	91	1	In	in	IN
cord-324094-23kzr8rq	91	2	addition	addition	NN
cord-324094-23kzr8rq	91	3	,	,	,
cord-324094-23kzr8rq	91	4	the	the	DT
cord-324094-23kzr8rq	91	5	ability	ability	NN
cord-324094-23kzr8rq	91	6	to	to	TO
cord-324094-23kzr8rq	91	7	quantify	quantify	VB
cord-324094-23kzr8rq	91	8	helped	help	VBD
cord-324094-23kzr8rq	91	9	elucidate	elucidate	VB
cord-324094-23kzr8rq	91	10	the	the	DT
cord-324094-23kzr8rq	91	11	pathogenesis	pathogenesis	NN
cord-324094-23kzr8rq	91	12	of	of	IN
cord-324094-23kzr8rq	91	13	the	the	DT
cord-324094-23kzr8rq	91	14	disease	disease	NN
cord-324094-23kzr8rq	91	15	.	.	.
cord-324094-23kzr8rq	92	1	QRTPCR	QRTPCR	NNP
cord-324094-23kzr8rq	92	2	assays	assay	NNS
cord-324094-23kzr8rq	92	3	can	can	MD
cord-324094-23kzr8rq	92	4	now	now	RB
cord-324094-23kzr8rq	92	5	be	be	VB
cord-324094-23kzr8rq	92	6	used	use	VBN
cord-324094-23kzr8rq	92	7	routinely	routinely	RB
cord-324094-23kzr8rq	92	8	to	to	TO
cord-324094-23kzr8rq	92	9	exclude	exclude	VB
cord-324094-23kzr8rq	92	10	SARS	SARS	NNP
cord-324094-23kzr8rq	92	11	-	-	HYPH
cord-324094-23kzr8rq	92	12	associated	associate	VBN
cord-324094-23kzr8rq	92	13	coronavirus	coronavirus	NN
cord-324094-23kzr8rq	92	14	in	in	IN
cord-324094-23kzr8rq	92	15	patients	patient	NNS
cord-324094-23kzr8rq	92	16	hospitalized	hospitalize	VBN
cord-324094-23kzr8rq	92	17	with	with	IN
cord-324094-23kzr8rq	92	18	respiratory	respiratory	JJ
cord-324094-23kzr8rq	92	19	symptoms	symptom	NNS
cord-324094-23kzr8rq	92	20	even	even	RB
cord-324094-23kzr8rq	92	21	in	in	IN
cord-324094-23kzr8rq	92	22	the	the	DT
cord-324094-23kzr8rq	92	23	presence	presence	NN
cord-324094-23kzr8rq	92	24	of	of	IN
cord-324094-23kzr8rq	92	25	other	other	JJ
cord-324094-23kzr8rq	92	26	respiratory	respiratory	JJ
cord-324094-23kzr8rq	92	27	viruses	virus	NNS
cord-324094-23kzr8rq	92	28	.	.	.
cord-324094-23kzr8rq	93	1	QRTPCR	QRTPCR	NNP
cord-324094-23kzr8rq	93	2	assays	assay	NNS
cord-324094-23kzr8rq	93	3	have	have	VBP
cord-324094-23kzr8rq	93	4	also	also	RB
cord-324094-23kzr8rq	93	5	been	be	VBN
cord-324094-23kzr8rq	93	6	developed	develop	VBN
cord-324094-23kzr8rq	93	7	for	for	IN
cord-324094-23kzr8rq	93	8	the	the	DT
cord-324094-23kzr8rq	93	9	clinical	clinical	JJ
cord-324094-23kzr8rq	93	10	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	93	11	of	of	IN
cord-324094-23kzr8rq	93	12	viral	viral	JJ
cord-324094-23kzr8rq	93	13	meningitis	meningitis	NN
cord-324094-23kzr8rq	93	14	and	and	CC
cord-324094-23kzr8rq	93	15	to	to	TO
cord-324094-23kzr8rq	93	16	detect	detect	VB
cord-324094-23kzr8rq	93	17	enteroviruses	enterovirus	NNS
cord-324094-23kzr8rq	93	18	in	in	IN
cord-324094-23kzr8rq	93	19	cerebrospinal	cerebrospinal	JJ
cord-324094-23kzr8rq	93	20	fl	fl	NNP
cord-324094-23kzr8rq	93	21	uid	uid	NN
cord-324094-23kzr8rq	93	22	and	and	CC
cord-324094-23kzr8rq	93	23	are	be	VBP
cord-324094-23kzr8rq	93	24	signifi	signifi	NNP
cord-324094-23kzr8rq	93	25	cantly	cantly	RB
cord-324094-23kzr8rq	93	26	more	more	RBR
cord-324094-23kzr8rq	93	27	sensitive	sensitive	JJ
cord-324094-23kzr8rq	93	28	than	than	IN
cord-324094-23kzr8rq	93	29	viral	viral	JJ
cord-324094-23kzr8rq	93	30	culture	culture	NN
cord-324094-23kzr8rq	93	31	(	(	-LRB-
cord-324094-23kzr8rq	93	32	Verstrepen	Verstrepen	NNP
cord-324094-23kzr8rq	93	33	et	et	FW
cord-324094-23kzr8rq	93	34	al	al	NNP
cord-324094-23kzr8rq	93	35	2001	2001	CD
cord-324094-23kzr8rq	93	36	;	;	:
cord-324094-23kzr8rq	93	37	Beuret	Beuret	NNP
cord-324094-23kzr8rq	93	38	2004	2004	CD
cord-324094-23kzr8rq	93	39	)	)	-RRB-
cord-324094-23kzr8rq	93	40	.	.	.
cord-324094-23kzr8rq	94	1	The	the	DT
cord-324094-23kzr8rq	94	2	ability	ability	NN
cord-324094-23kzr8rq	94	3	of	of	IN
cord-324094-23kzr8rq	94	4	qPCR	qpcr	NN
cord-324094-23kzr8rq	94	5	to	to	TO
cord-324094-23kzr8rq	94	6	generate	generate	VB
cord-324094-23kzr8rq	94	7	accurate	accurate	JJ
cord-324094-23kzr8rq	94	8	quantitative	quantitative	JJ
cord-324094-23kzr8rq	94	9	data	datum	NNS
cord-324094-23kzr8rq	94	10	has	have	VBZ
cord-324094-23kzr8rq	94	11	had	have	VBN
cord-324094-23kzr8rq	94	12	a	a	DT
cord-324094-23kzr8rq	94	13	huge	huge	JJ
cord-324094-23kzr8rq	94	14	impact	impact	NN
cord-324094-23kzr8rq	94	15	on	on	IN
cord-324094-23kzr8rq	94	16	the	the	DT
cord-324094-23kzr8rq	94	17	study	study	NN
cord-324094-23kzr8rq	94	18	of	of	IN
cord-324094-23kzr8rq	94	19	viral	viral	JJ
cord-324094-23kzr8rq	94	20	agents	agent	NNS
cord-324094-23kzr8rq	94	21	of	of	IN
cord-324094-23kzr8rq	94	22	infectious	infectious	JJ
cord-324094-23kzr8rq	94	23	disease	disease	NN
cord-324094-23kzr8rq	94	24	and	and	CC
cord-324094-23kzr8rq	94	25	is	be	VBZ
cord-324094-23kzr8rq	94	26	helping	help	VBG
cord-324094-23kzr8rq	94	27	to	to	TO
cord-324094-23kzr8rq	94	28	clarify	clarify	VB
cord-324094-23kzr8rq	94	29	disputed	dispute	VBN
cord-324094-23kzr8rq	94	30	infectious	infectious	JJ
cord-324094-23kzr8rq	94	31	disease	disease	NN
cord-324094-23kzr8rq	94	32	processes	process	NNS
cord-324094-23kzr8rq	94	33	and	and	CC
cord-324094-23kzr8rq	94	34	demonstrate	demonstrate	VB
cord-324094-23kzr8rq	94	35	links	link	NNS
cord-324094-23kzr8rq	94	36	between	between	IN
cord-324094-23kzr8rq	94	37	specifi	specifi	NN
cord-324094-23kzr8rq	94	38	c	c	NN
cord-324094-23kzr8rq	94	39	viral	viral	JJ
cord-324094-23kzr8rq	94	40	sequences	sequence	NNS
cord-324094-23kzr8rq	94	41	and	and	CC
cord-324094-23kzr8rq	94	42	patient	patient	JJ
cord-324094-23kzr8rq	94	43	clinical	clinical	JJ
cord-324094-23kzr8rq	94	44	symptoms	symptom	NNS
cord-324094-23kzr8rq	94	45	.	.	.
cord-324094-23kzr8rq	95	1	However	however	RB
cord-324094-23kzr8rq	95	2	,	,	,
cord-324094-23kzr8rq	95	3	the	the	DT
cord-324094-23kzr8rq	95	4	lack	lack	NN
cord-324094-23kzr8rq	95	5	of	of	IN
cord-324094-23kzr8rq	95	6	commercially	commercially	RB
cord-324094-23kzr8rq	95	7	available	available	JJ
cord-324094-23kzr8rq	95	8	validated	validate	VBN
cord-324094-23kzr8rq	95	9	reagent	reagent	NN
cord-324094-23kzr8rq	95	10	kits	kit	NNS
cord-324094-23kzr8rq	95	11	for	for	IN
cord-324094-23kzr8rq	95	12	most	most	JJS
cord-324094-23kzr8rq	95	13	viruses	virus	NNS
cord-324094-23kzr8rq	95	14	remains	remain	VBZ
cord-324094-23kzr8rq	95	15	a	a	DT
cord-324094-23kzr8rq	95	16	major	major	JJ
cord-324094-23kzr8rq	95	17	problem	problem	NN
cord-324094-23kzr8rq	95	18	,	,	,
cord-324094-23kzr8rq	95	19	as	as	IN
cord-324094-23kzr8rq	95	20	does	do	VBZ
cord-324094-23kzr8rq	95	21	the	the	DT
cord-324094-23kzr8rq	95	22	absence	absence	NN
cord-324094-23kzr8rq	95	23	of	of	IN
cord-324094-23kzr8rq	95	24	standardization	standardization	NN
cord-324094-23kzr8rq	95	25	of	of	IN
cord-324094-23kzr8rq	95	26	the	the	DT
cord-324094-23kzr8rq	95	27	existing	exist	VBG
cord-324094-23kzr8rq	95	28	tests	test	NNS
cord-324094-23kzr8rq	95	29	(	(	-LRB-
cord-324094-23kzr8rq	95	30	Niesters	Niesters	NNP
cord-324094-23kzr8rq	95	31	2002	2002	CD
cord-324094-23kzr8rq	95	32	)	)	-RRB-
cord-324094-23kzr8rq	95	33	.	.	.
cord-324094-23kzr8rq	96	1	Besides	besides	RB
cord-324094-23kzr8rq	96	2	,	,	,
cord-324094-23kzr8rq	96	3	all	all	PDT
cord-324094-23kzr8rq	96	4	these	these	DT
cord-324094-23kzr8rq	96	5	nucleic	nucleic	JJ
cord-324094-23kzr8rq	96	6	acid	acid	NN
cord-324094-23kzr8rq	96	7	amplifi	amplifi	NNP
cord-324094-23kzr8rq	97	1	cation	cation	NN
cord-324094-23kzr8rq	97	2	methods	method	NNS
cord-324094-23kzr8rq	97	3	have	have	VBP
cord-324094-23kzr8rq	97	4	several	several	JJ
cord-324094-23kzr8rq	97	5	intrinsic	intrinsic	JJ
cord-324094-23kzr8rq	97	6	disadvantages	disadvantage	NNS
cord-324094-23kzr8rq	97	7	of	of	IN
cord-324094-23kzr8rq	97	8	requiring	require	VBG
cord-324094-23kzr8rq	97	9	either	either	CC
cord-324094-23kzr8rq	97	10	a	a	DT
cord-324094-23kzr8rq	97	11	high	high	JJ
cord-324094-23kzr8rq	97	12	precision	precision	NN
cord-324094-23kzr8rq	97	13	instrument	instrument	NN
cord-324094-23kzr8rq	97	14	for	for	IN
cord-324094-23kzr8rq	97	15	amplifi	amplifi	NNP
cord-324094-23kzr8rq	97	16	cation	cation	NN
cord-324094-23kzr8rq	97	17	or	or	CC
cord-324094-23kzr8rq	97	18	an	an	DT
cord-324094-23kzr8rq	97	19	elaborate	elaborate	JJ
cord-324094-23kzr8rq	97	20	complicated	complicated	JJ
cord-324094-23kzr8rq	97	21	method	method	NN
cord-324094-23kzr8rq	97	22	for	for	IN
cord-324094-23kzr8rq	97	23	detection	detection	NN
cord-324094-23kzr8rq	97	24	of	of	IN
cord-324094-23kzr8rq	97	25	amplifi	amplifi	NNP
cord-324094-23kzr8rq	97	26	ed	ed	NNP
cord-324094-23kzr8rq	97	27	products	product	NNS
cord-324094-23kzr8rq	97	28	.	.	.
cord-324094-23kzr8rq	98	1	The	the	DT
cord-324094-23kzr8rq	98	2	high	high	JJ
cord-324094-23kzr8rq	98	3	cost	cost	NN
cord-324094-23kzr8rq	98	4	of	of	IN
cord-324094-23kzr8rq	98	5	instruments	instrument	NNS
cord-324094-23kzr8rq	98	6	required	require	VBN
cord-324094-23kzr8rq	98	7	for	for	IN
cord-324094-23kzr8rq	98	8	performing	perform	VBG
cord-324094-23kzr8rq	98	9	the	the	DT
cord-324094-23kzr8rq	98	10	real	real	JJ
cord-324094-23kzr8rq	98	11	time	time	NN
cord-324094-23kzr8rq	98	12	assays	assay	NNS
cord-324094-23kzr8rq	98	13	restricted	restrict	VBD
cord-324094-23kzr8rq	98	14	its	-PRON-	PRP$
cord-324094-23kzr8rq	98	15	use	use	NN
cord-324094-23kzr8rq	98	16	to	to	IN
cord-324094-23kzr8rq	98	17	laboratories	laboratory	NNS
cord-324094-23kzr8rq	98	18	with	with	IN
cord-324094-23kzr8rq	98	19	good	good	JJ
cord-324094-23kzr8rq	98	20	fi	fi	NN
cord-324094-23kzr8rq	98	21	nancial	nancial	JJ
cord-324094-23kzr8rq	98	22	resources	resource	NNS
cord-324094-23kzr8rq	98	23	.	.	.
cord-324094-23kzr8rq	99	1	Loop	loop	NN
cord-324094-23kzr8rq	99	2	-	-	HYPH
cord-324094-23kzr8rq	99	3	mediated	mediate	VBN
cord-324094-23kzr8rq	99	4	isothermal	isothermal	JJ
cord-324094-23kzr8rq	99	5	amplifi	amplifi	NNP
cord-324094-23kzr8rq	99	6	cation	cation	NN
cord-324094-23kzr8rq	99	7	(	(	-LRB-
cord-324094-23kzr8rq	99	8	LAMP	LAMP	NNP
cord-324094-23kzr8rq	99	9	)	)	-RRB-
cord-324094-23kzr8rq	99	10	which	which	WDT
cord-324094-23kzr8rq	99	11	stands	stand	VBZ
cord-324094-23kzr8rq	99	12	for	for	IN
cord-324094-23kzr8rq	99	13	loop	loop	NN
cord-324094-23kzr8rq	99	14	-	-	HYPH
cord-324094-23kzr8rq	99	15	mediated	mediate	VBN
cord-324094-23kzr8rq	99	16	isothermal	isothermal	JJ
cord-324094-23kzr8rq	99	17	amplifi	amplifi	NNP
cord-324094-23kzr8rq	99	18	cation	cation	NN
cord-324094-23kzr8rq	99	19	is	be	VBZ
cord-324094-23kzr8rq	99	20	a	a	DT
cord-324094-23kzr8rq	99	21	simple	simple	JJ
cord-324094-23kzr8rq	99	22	,	,	,
cord-324094-23kzr8rq	99	23	rapid	rapid	JJ
cord-324094-23kzr8rq	99	24	,	,	,
cord-324094-23kzr8rq	99	25	specifi	specifi	NNP
cord-324094-23kzr8rq	99	26	c	c	NN
cord-324094-23kzr8rq	99	27	and	and	CC
cord-324094-23kzr8rq	99	28	cost	cost	NN
cord-324094-23kzr8rq	99	29	-	-	HYPH
cord-324094-23kzr8rq	99	30	effective	effective	JJ
cord-324094-23kzr8rq	99	31	nucleic	nucleic	JJ
cord-324094-23kzr8rq	99	32	acid	acid	NN
cord-324094-23kzr8rq	99	33	amplifi	amplifi	NNP
cord-324094-23kzr8rq	99	34	cation	cation	NN
cord-324094-23kzr8rq	99	35	method	method	NN
cord-324094-23kzr8rq	99	36	and	and	CC
cord-324094-23kzr8rq	99	37	is	be	VBZ
cord-324094-23kzr8rq	99	38	characterized	characterize	VBN
cord-324094-23kzr8rq	99	39	by	by	IN
cord-324094-23kzr8rq	99	40	the	the	DT
cord-324094-23kzr8rq	99	41	use	use	NN
cord-324094-23kzr8rq	99	42	of	of	IN
cord-324094-23kzr8rq	99	43	6	6	CD
cord-324094-23kzr8rq	99	44	different	different	JJ
cord-324094-23kzr8rq	99	45	primers	primer	NNS
cord-324094-23kzr8rq	99	46	specifi	specifi	VBN
cord-324094-23kzr8rq	99	47	cally	cally	RB
cord-324094-23kzr8rq	99	48	designed	design	VBN
cord-324094-23kzr8rq	99	49	to	to	TO
cord-324094-23kzr8rq	99	50	recognize	recognize	VB
cord-324094-23kzr8rq	99	51	8	8	CD
cord-324094-23kzr8rq	99	52	distinct	distinct	JJ
cord-324094-23kzr8rq	99	53	regions	region	NNS
cord-324094-23kzr8rq	99	54	on	on	IN
cord-324094-23kzr8rq	99	55	the	the	DT
cord-324094-23kzr8rq	99	56	target	target	NN
cord-324094-23kzr8rq	99	57	gene	gene	NN
cord-324094-23kzr8rq	99	58	(	(	-LRB-
cord-324094-23kzr8rq	99	59	Notomi	Notomi	NNP
cord-324094-23kzr8rq	99	60	et	et	NNP
cord-324094-23kzr8rq	99	61	al	al	NNP
cord-324094-23kzr8rq	99	62	2000	2000	CD
cord-324094-23kzr8rq	99	63	)	)	-RRB-
cord-324094-23kzr8rq	99	64	.	.	.
cord-324094-23kzr8rq	100	1	The	the	DT
cord-324094-23kzr8rq	100	2	amplifi	amplifi	NNP
cord-324094-23kzr8rq	100	3	cation	cation	NN
cord-324094-23kzr8rq	100	4	proceeds	proceed	VBZ
cord-324094-23kzr8rq	100	5	at	at	IN
cord-324094-23kzr8rq	100	6	a	a	DT
cord-324094-23kzr8rq	100	7	constant	constant	JJ
cord-324094-23kzr8rq	100	8	temperature	temperature	NN
cord-324094-23kzr8rq	100	9	using	use	VBG
cord-324094-23kzr8rq	100	10	strand	strand	NN
cord-324094-23kzr8rq	100	11	displacement	displacement	NN
cord-324094-23kzr8rq	100	12	reaction	reaction	NN
cord-324094-23kzr8rq	100	13	.	.	.
cord-324094-23kzr8rq	101	1	Amplifi	Amplifi	NNP
cord-324094-23kzr8rq	101	2	cation	cation	NN
cord-324094-23kzr8rq	101	3	and	and	CC
cord-324094-23kzr8rq	101	4	detection	detection	NN
cord-324094-23kzr8rq	101	5	of	of	IN
cord-324094-23kzr8rq	101	6	gene	gene	NN
cord-324094-23kzr8rq	101	7	can	can	MD
cord-324094-23kzr8rq	101	8	be	be	VB
cord-324094-23kzr8rq	101	9	completed	complete	VBN
cord-324094-23kzr8rq	101	10	in	in	IN
cord-324094-23kzr8rq	101	11	a	a	DT
cord-324094-23kzr8rq	101	12	single	single	JJ
cord-324094-23kzr8rq	101	13	step	step	NN
cord-324094-23kzr8rq	101	14	,	,	,
cord-324094-23kzr8rq	101	15	by	by	IN
cord-324094-23kzr8rq	101	16	incubating	incubate	VBG
cord-324094-23kzr8rq	101	17	the	the	DT
cord-324094-23kzr8rq	101	18	mixture	mixture	NN
cord-324094-23kzr8rq	101	19	of	of	IN
cord-324094-23kzr8rq	101	20	samples	sample	NNS
cord-324094-23kzr8rq	101	21	,	,	,
cord-324094-23kzr8rq	101	22	primers	primer	NNS
cord-324094-23kzr8rq	101	23	,	,	,
cord-324094-23kzr8rq	101	24	DNA	dna	NN
cord-324094-23kzr8rq	101	25	polymerase	polymerase	NN
cord-324094-23kzr8rq	101	26	with	with	IN
cord-324094-23kzr8rq	101	27	strand	strand	NN
cord-324094-23kzr8rq	101	28	displacement	displacement	NN
cord-324094-23kzr8rq	101	29	activity	activity	NN
cord-324094-23kzr8rq	101	30	and	and	CC
cord-324094-23kzr8rq	101	31	substrates	substrate	NNS
cord-324094-23kzr8rq	101	32	at	at	IN
cord-324094-23kzr8rq	101	33	a	a	DT
cord-324094-23kzr8rq	101	34	constant	constant	JJ
cord-324094-23kzr8rq	101	35	temperature	temperature	NN
cord-324094-23kzr8rq	101	36	of	of	IN
cord-324094-23kzr8rq	101	37	63	63	CD
cord-324094-23kzr8rq	101	38	°	°	NN
cord-324094-23kzr8rq	101	39	C	c	NN
cord-324094-23kzr8rq	101	40	.	.	.
cord-324094-23kzr8rq	102	1	Compared	compare	VBN
cord-324094-23kzr8rq	102	2	to	to	IN
cord-324094-23kzr8rq	102	3	PCR	PCR	NNP
cord-324094-23kzr8rq	102	4	and	and	CC
cord-324094-23kzr8rq	102	5	real	real	JJ
cord-324094-23kzr8rq	102	6	-	-	HYPH
cord-324094-23kzr8rq	102	7	time	time	NN
cord-324094-23kzr8rq	102	8	PCR	PCR	NNP
cord-324094-23kzr8rq	102	9	,	,	,
cord-324094-23kzr8rq	102	10	the	the	DT
cord-324094-23kzr8rq	102	11	LAMP	LAMP	NNP
cord-324094-23kzr8rq	102	12	has	have	VBZ
cord-324094-23kzr8rq	102	13	the	the	DT
cord-324094-23kzr8rq	102	14	advantages	advantage	NNS
cord-324094-23kzr8rq	102	15	of	of	IN
cord-324094-23kzr8rq	102	16	reaction	reaction	NN
cord-324094-23kzr8rq	102	17	simplicity	simplicity	NN
cord-324094-23kzr8rq	102	18	and	and	CC
cord-324094-23kzr8rq	102	19	detection	detection	NN
cord-324094-23kzr8rq	102	20	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	102	21	.	.	.
cord-324094-23kzr8rq	103	1	The	the	DT
cord-324094-23kzr8rq	103	2	higher	high	JJR
cord-324094-23kzr8rq	103	3	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	103	4	and	and	CC
cord-324094-23kzr8rq	103	5	specifi	specifi	NN
cord-324094-23kzr8rq	103	6	city	city	NN
cord-324094-23kzr8rq	103	7	of	of	IN
cord-324094-23kzr8rq	103	8	the	the	DT
cord-324094-23kzr8rq	103	9	LAMP	LAMP	NNP
cord-324094-23kzr8rq	103	10	reaction	reaction	NN
cord-324094-23kzr8rq	103	11	is	be	VBZ
cord-324094-23kzr8rq	103	12	attributed	attribute	VBN
cord-324094-23kzr8rq	103	13	to	to	IN
cord-324094-23kzr8rq	103	14	continuous	continuous	JJ
cord-324094-23kzr8rq	103	15	amplifi	amplifi	NNP
cord-324094-23kzr8rq	103	16	cation	cation	NN
cord-324094-23kzr8rq	103	17	under	under	IN
cord-324094-23kzr8rq	103	18	isothermal	isothermal	JJ
cord-324094-23kzr8rq	103	19	condition	condition	NN
cord-324094-23kzr8rq	103	20	employing	employ	VBG
cord-324094-23kzr8rq	103	21	six	six	CD
cord-324094-23kzr8rq	103	22	primers	primer	NNS
cord-324094-23kzr8rq	103	23	recognizing	recognize	VBG
cord-324094-23kzr8rq	103	24	eight	eight	CD
cord-324094-23kzr8rq	103	25	distinct	distinct	JJ
cord-324094-23kzr8rq	103	26	regions	region	NNS
cord-324094-23kzr8rq	103	27	of	of	IN
cord-324094-23kzr8rq	103	28	the	the	DT
cord-324094-23kzr8rq	103	29	target	target	NN
cord-324094-23kzr8rq	103	30	.	.	.
cord-324094-23kzr8rq	104	1	Besides	besides	RB
cord-324094-23kzr8rq	104	2	,	,	,
cord-324094-23kzr8rq	104	3	the	the	DT
cord-324094-23kzr8rq	104	4	higher	high	JJR
cord-324094-23kzr8rq	104	5	amplifi	amplifi	NNP
cord-324094-23kzr8rq	104	6	cation	cation	NNP
cord-324094-23kzr8rq	104	7	effi	effi	NNP
cord-324094-23kzr8rq	104	8	ciency	ciency	NNP
cord-324094-23kzr8rq	104	9	of	of	IN
cord-324094-23kzr8rq	104	10	LAMP	LAMP	NNP
cord-324094-23kzr8rq	104	11	reaction	reaction	NN
cord-324094-23kzr8rq	104	12	yields	yield	VBZ
cord-324094-23kzr8rq	104	13	large	large	JJ
cord-324094-23kzr8rq	104	14	amount	amount	NN
cord-324094-23kzr8rq	104	15	of	of	IN
cord-324094-23kzr8rq	104	16	byproduct	byproduct	NN
cord-324094-23kzr8rq	104	17	,	,	,
cord-324094-23kzr8rq	104	18	pyrophosphate	pyrophosphate	NN
cord-324094-23kzr8rq	104	19	ion	ion	NN
cord-324094-23kzr8rq	104	20	,	,	,
cord-324094-23kzr8rq	104	21	leading	lead	VBG
cord-324094-23kzr8rq	104	22	to	to	IN
cord-324094-23kzr8rq	104	23	white	white	JJ
cord-324094-23kzr8rq	104	24	precipitate	precipitate	NN
cord-324094-23kzr8rq	104	25	of	of	IN
cord-324094-23kzr8rq	104	26	magnesium	magnesium	NN
cord-324094-23kzr8rq	104	27	pyrophosphate	pyrophosphate	NN
cord-324094-23kzr8rq	104	28	in	in	IN
cord-324094-23kzr8rq	104	29	the	the	DT
cord-324094-23kzr8rq	104	30	reaction	reaction	NN
cord-324094-23kzr8rq	104	31	mixture	mixture	NN
cord-324094-23kzr8rq	104	32	.	.	.
cord-324094-23kzr8rq	105	1	Since	since	IN
cord-324094-23kzr8rq	105	2	the	the	DT
cord-324094-23kzr8rq	105	3	increase	increase	NN
cord-324094-23kzr8rq	105	4	in	in	IN
cord-324094-23kzr8rq	105	5	turbidity	turbidity	NN
cord-324094-23kzr8rq	105	6	of	of	IN
cord-324094-23kzr8rq	105	7	the	the	DT
cord-324094-23kzr8rq	105	8	reaction	reaction	NN
cord-324094-23kzr8rq	105	9	mixture	mixture	NN
cord-324094-23kzr8rq	105	10	according	accord	VBG
cord-324094-23kzr8rq	105	11	to	to	IN
cord-324094-23kzr8rq	105	12	the	the	DT
cord-324094-23kzr8rq	105	13	production	production	NN
cord-324094-23kzr8rq	105	14	of	of	IN
cord-324094-23kzr8rq	105	15	precipitate	precipitate	NN
cord-324094-23kzr8rq	105	16	correlates	correlate	VBZ
cord-324094-23kzr8rq	105	17	with	with	IN
cord-324094-23kzr8rq	105	18	the	the	DT
cord-324094-23kzr8rq	105	19	amount	amount	NN
cord-324094-23kzr8rq	105	20	of	of	IN
cord-324094-23kzr8rq	105	21	DNA	dna	NN
cord-324094-23kzr8rq	106	1	synthesized	synthesize	VBN
cord-324094-23kzr8rq	106	2	,	,	,
cord-324094-23kzr8rq	106	3	real	real	JJ
cord-324094-23kzr8rq	106	4	-	-	HYPH
cord-324094-23kzr8rq	106	5	time	time	NN
cord-324094-23kzr8rq	106	6	monitoring	monitoring	NN
cord-324094-23kzr8rq	106	7	of	of	IN
cord-324094-23kzr8rq	106	8	the	the	DT
cord-324094-23kzr8rq	106	9	LAMP	LAMP	NNP
cord-324094-23kzr8rq	106	10	reaction	reaction	NN
cord-324094-23kzr8rq	106	11	can	can	MD
cord-324094-23kzr8rq	106	12	be	be	VB
cord-324094-23kzr8rq	106	13	achieved	achieve	VBN
cord-324094-23kzr8rq	106	14	by	by	IN
cord-324094-23kzr8rq	106	15	real	real	JJ
cord-324094-23kzr8rq	106	16	-	-	HYPH
cord-324094-23kzr8rq	106	17	time	time	NN
cord-324094-23kzr8rq	106	18	measurement	measurement	NN
cord-324094-23kzr8rq	106	19	of	of	IN
cord-324094-23kzr8rq	106	20	turbidity	turbidity	NN
cord-324094-23kzr8rq	106	21	(	(	-LRB-
cord-324094-23kzr8rq	106	22	Mori	Mori	NNP
cord-324094-23kzr8rq	106	23	et	et	FW
cord-324094-23kzr8rq	106	24	al	al	NNP
cord-324094-23kzr8rq	106	25	2001	2001	CD
cord-324094-23kzr8rq	106	26	)	)	-RRB-
cord-324094-23kzr8rq	106	27	.	.	.
cord-324094-23kzr8rq	107	1	Being	be	VBG
cord-324094-23kzr8rq	107	2	an	an	DT
cord-324094-23kzr8rq	107	3	isothermal	isothermal	JJ
cord-324094-23kzr8rq	107	4	amplifi	amplifi	NNP
cord-324094-23kzr8rq	107	5	cation	cation	NN
cord-324094-23kzr8rq	107	6	,	,	,
cord-324094-23kzr8rq	107	7	LAMP	LAMP	NNP
cord-324094-23kzr8rq	107	8	does	do	VBZ
cord-324094-23kzr8rq	107	9	not	not	RB
cord-324094-23kzr8rq	107	10	require	require	VB
cord-324094-23kzr8rq	107	11	any	any	DT
cord-324094-23kzr8rq	107	12	thermal	thermal	JJ
cord-324094-23kzr8rq	107	13	cycler	cycler	NN
cord-324094-23kzr8rq	107	14	and	and	CC
cord-324094-23kzr8rq	107	15	thus	thus	RB
cord-324094-23kzr8rq	107	16	can	can	MD
cord-324094-23kzr8rq	107	17	be	be	VB
cord-324094-23kzr8rq	107	18	performed	perform	VBN
cord-324094-23kzr8rq	107	19	even	even	RB
cord-324094-23kzr8rq	107	20	with	with	IN
cord-324094-23kzr8rq	107	21	heating	heating	NN
cord-324094-23kzr8rq	107	22	block	block	NN
cord-324094-23kzr8rq	107	23	and/or	and/or	CC
cord-324094-23kzr8rq	107	24	water	water	NN
cord-324094-23kzr8rq	107	25	bath	bath	NN
cord-324094-23kzr8rq	107	26	.	.	.
cord-324094-23kzr8rq	108	1	Thus	thus	RB
cord-324094-23kzr8rq	108	2	,	,	,
cord-324094-23kzr8rq	108	3	LAMP	lamp	NN
cord-324094-23kzr8rq	108	4	method	method	NN
cord-324094-23kzr8rq	108	5	has	have	VBZ
cord-324094-23kzr8rq	108	6	the	the	DT
cord-324094-23kzr8rq	108	7	characteristics	characteristic	NNS
cord-324094-23kzr8rq	108	8	of	of	IN
cord-324094-23kzr8rq	108	9	not	not	RB
cord-324094-23kzr8rq	108	10	requiring	require	VBG
cord-324094-23kzr8rq	108	11	special	special	JJ
cord-324094-23kzr8rq	108	12	reagents	reagent	NNS
cord-324094-23kzr8rq	108	13	and	and	CC
cord-324094-23kzr8rq	108	14	sophisticated	sophisticated	JJ
cord-324094-23kzr8rq	108	15	temperature	temperature	NN
cord-324094-23kzr8rq	108	16	control	control	NN
cord-324094-23kzr8rq	108	17	device	device	NN
cord-324094-23kzr8rq	108	18	.	.	.
cord-324094-23kzr8rq	109	1	Since	since	IN
cord-324094-23kzr8rq	109	2	it	-PRON-	PRP
cord-324094-23kzr8rq	109	3	only	only	RB
cord-324094-23kzr8rq	109	4	requires	require	VBZ
cord-324094-23kzr8rq	109	5	simple	simple	JJ
cord-324094-23kzr8rq	109	6	equipments	equipment	NNS
cord-324094-23kzr8rq	109	7	,	,	,
cord-324094-23kzr8rq	109	8	cost	cost	VBD
cord-324094-23kzr8rq	109	9	effective	effective	JJ
cord-324094-23kzr8rq	109	10	point	point	NN
cord-324094-23kzr8rq	109	11	of	of	IN
cord-324094-23kzr8rq	109	12	care	care	NN
cord-324094-23kzr8rq	109	13	gene	gene	NN
cord-324094-23kzr8rq	109	14	test	test	NN
cord-324094-23kzr8rq	109	15	can	can	MD
cord-324094-23kzr8rq	109	16	be	be	VB
cord-324094-23kzr8rq	109	17	achieved	achieve	VBN
cord-324094-23kzr8rq	109	18	.	.	.
cord-324094-23kzr8rq	110	1	Both	both	DT
cord-324094-23kzr8rq	110	2	simple	simple	JJ
cord-324094-23kzr8rq	110	3	detection	detection	NN
cord-324094-23kzr8rq	110	4	and	and	CC
cord-324094-23kzr8rq	110	5	real	real	JJ
cord-324094-23kzr8rq	110	6	-	-	HYPH
cord-324094-23kzr8rq	110	7	time	time	NN
cord-324094-23kzr8rq	110	8	detection	detection	NN
cord-324094-23kzr8rq	110	9	of	of	IN
cord-324094-23kzr8rq	110	10	the	the	DT
cord-324094-23kzr8rq	110	11	reaction	reaction	NN
cord-324094-23kzr8rq	110	12	are	be	VBP
cord-324094-23kzr8rq	110	13	possible	possible	JJ
cord-324094-23kzr8rq	110	14	.	.	.
cord-324094-23kzr8rq	111	1	In	in	IN
cord-324094-23kzr8rq	111	2	addition	addition	NN
cord-324094-23kzr8rq	111	3	,	,	,
cord-324094-23kzr8rq	111	4	in	in	IN
cord-324094-23kzr8rq	111	5	case	case	NN
cord-324094-23kzr8rq	111	6	of	of	IN
cord-324094-23kzr8rq	111	7	LAMP	LAMP	NNP
cord-324094-23kzr8rq	111	8	,	,	,
cord-324094-23kzr8rq	111	9	both	both	DT
cord-324094-23kzr8rq	111	10	amplifi	amplifi	NNP
cord-324094-23kzr8rq	111	11	cation	cation	NN
cord-324094-23kzr8rq	111	12	and	and	CC
cord-324094-23kzr8rq	111	13	detection	detection	NN
cord-324094-23kzr8rq	111	14	occur	occur	VBP
cord-324094-23kzr8rq	111	15	simultaneously	simultaneously	RB
cord-324094-23kzr8rq	111	16	during	during	IN
cord-324094-23kzr8rq	111	17	the	the	DT
cord-324094-23kzr8rq	111	18	exponential	exponential	JJ
cord-324094-23kzr8rq	111	19	phase	phase	NN
cord-324094-23kzr8rq	111	20	without	without	IN
cord-324094-23kzr8rq	111	21	going	go	VBG
cord-324094-23kzr8rq	111	22	through	through	IN
cord-324094-23kzr8rq	111	23	the	the	DT
cord-324094-23kzr8rq	111	24	plateau	plateau	NN
cord-324094-23kzr8rq	111	25	phase	phase	NN
cord-324094-23kzr8rq	111	26	where	where	WRB
cord-324094-23kzr8rq	111	27	the	the	DT
cord-324094-23kzr8rq	111	28	non	non	AFX
cord-324094-23kzr8rq	111	29	spurious	spurious	JJ
cord-324094-23kzr8rq	111	30	amplifi	amplifi	NNP
cord-324094-23kzr8rq	111	31	cation	cation	NN
cord-324094-23kzr8rq	111	32	leads	lead	VBZ
cord-324094-23kzr8rq	111	33	to	to	TO
cord-324094-23kzr8rq	111	34	lower	lower	VB
cord-324094-23kzr8rq	111	35	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	111	36	and	and	CC
cord-324094-23kzr8rq	111	37	false	false	JJ
cord-324094-23kzr8rq	111	38	positivity	positivity	NN
cord-324094-23kzr8rq	111	39	.	.	.
cord-324094-23kzr8rq	112	1	Designing	design	VBG
cord-324094-23kzr8rq	112	2	of	of	IN
cord-324094-23kzr8rq	112	3	a	a	DT
cord-324094-23kzr8rq	112	4	highly	highly	RB
cord-324094-23kzr8rq	112	5	sensitive	sensitive	JJ
cord-324094-23kzr8rq	112	6	and	and	CC
cord-324094-23kzr8rq	112	7	specifi	specifi	JJ
cord-324094-23kzr8rq	112	8	c	c	NN
cord-324094-23kzr8rq	112	9	primer	primer	NN
cord-324094-23kzr8rq	112	10	set	set	NN
cord-324094-23kzr8rq	112	11	is	be	VBZ
cord-324094-23kzr8rq	112	12	crucial	crucial	JJ
cord-324094-23kzr8rq	112	13	for	for	IN
cord-324094-23kzr8rq	112	14	performing	perform	VBG
cord-324094-23kzr8rq	112	15	LAMP	LAMP	NNP
cord-324094-23kzr8rq	112	16	amplifi	amplifi	NNP
cord-324094-23kzr8rq	112	17	cation	cation	NN
cord-324094-23kzr8rq	112	18	.	.	.
cord-324094-23kzr8rq	113	1	The	the	DT
cord-324094-23kzr8rq	113	2	target	target	NN
cord-324094-23kzr8rq	113	3	selection	selection	NN
cord-324094-23kzr8rq	113	4	for	for	IN
cord-324094-23kzr8rq	113	5	primer	primer	NN
cord-324094-23kzr8rq	113	6	designing	designing	NN
cord-324094-23kzr8rq	113	7	can	can	MD
cord-324094-23kzr8rq	113	8	be	be	VB
cord-324094-23kzr8rq	113	9	accomplished	accomplish	VBN
cord-324094-23kzr8rq	113	10	by	by	IN
cord-324094-23kzr8rq	113	11	using	use	VBG
cord-324094-23kzr8rq	113	12	the	the	DT
cord-324094-23kzr8rq	113	13	Primer	Primer	NNP
cord-324094-23kzr8rq	113	14	Explore	explore	VB
cord-324094-23kzr8rq	113	15	[	[	-LRB-
cord-324094-23kzr8rq	113	16	LAMP	lamp	NN
cord-324094-23kzr8rq	113	17	primer	primer	NN
cord-324094-23kzr8rq	113	18	designing	design	VBG
cord-324094-23kzr8rq	113	19	support	support	NN
cord-324094-23kzr8rq	113	20	software	software	NN
cord-324094-23kzr8rq	113	21	program	program	NN
cord-324094-23kzr8rq	113	22	,	,	,
cord-324094-23kzr8rq	113	23	Net	net	JJ
cord-324094-23kzr8rq	113	24	laboratory	laboratory	NN
cord-324094-23kzr8rq	113	25	,	,	,
cord-324094-23kzr8rq	113	26	Japan	Japan	NNP
cord-324094-23kzr8rq	113	27	,	,	,
cord-324094-23kzr8rq	113	28	http://venus.net	http://venus.net	ADD
cord-324094-23kzr8rq	113	29	laboratory.com	laboratory.com	ADD
cord-324094-23kzr8rq	113	30	)	)	-RRB-
cord-324094-23kzr8rq	113	31	]	]	-RRB-
cord-324094-23kzr8rq	114	1	after	after	IN
cord-324094-23kzr8rq	114	2	considering	consider	VBG
cord-324094-23kzr8rq	114	3	the	the	DT
cord-324094-23kzr8rq	114	4	base	base	NN
cord-324094-23kzr8rq	114	5	composition	composition	NN
cord-324094-23kzr8rq	114	6	,	,	,
cord-324094-23kzr8rq	114	7	GC	GC	NNP
cord-324094-23kzr8rq	114	8	contents	content	NNS
cord-324094-23kzr8rq	114	9	and	and	CC
cord-324094-23kzr8rq	114	10	the	the	DT
cord-324094-23kzr8rq	114	11	formation	formation	NN
cord-324094-23kzr8rq	114	12	of	of	IN
cord-324094-23kzr8rq	114	13	secondary	secondary	JJ
cord-324094-23kzr8rq	114	14	structures	structure	NNS
cord-324094-23kzr8rq	114	15	.	.	.
cord-324094-23kzr8rq	115	1	The	the	DT
cord-324094-23kzr8rq	115	2	primer	primer	NN
cord-324094-23kzr8rq	115	3	set	set	VBN
cord-324094-23kzr8rq	115	4	for	for	IN
cord-324094-23kzr8rq	115	5	LAMP	lamp	NN
cord-324094-23kzr8rq	115	6	amplifi	amplifi	NNP
cord-324094-23kzr8rq	116	1	cation	cation	NN
cord-324094-23kzr8rq	116	2	include	include	VBP
cord-324094-23kzr8rq	116	3	a	a	DT
cord-324094-23kzr8rq	116	4	set	set	NN
cord-324094-23kzr8rq	116	5	of	of	IN
cord-324094-23kzr8rq	116	6	six	six	CD
cord-324094-23kzr8rq	116	7	primers	primer	NNS
cord-324094-23kzr8rq	116	8	comprising	comprise	VBG
cord-324094-23kzr8rq	116	9	two	two	CD
cord-324094-23kzr8rq	116	10	outer	outer	JJ
cord-324094-23kzr8rq	116	11	,	,	,
cord-324094-23kzr8rq	116	12	two	two	CD
cord-324094-23kzr8rq	116	13	inner	inner	JJ
cord-324094-23kzr8rq	116	14	and	and	CC
cord-324094-23kzr8rq	116	15	two	two	CD
cord-324094-23kzr8rq	116	16	loop	loop	NN
cord-324094-23kzr8rq	116	17	primers	primer	NNS
cord-324094-23kzr8rq	116	18	that	that	WDT
cord-324094-23kzr8rq	116	19	recognize	recognize	VBP
cord-324094-23kzr8rq	116	20	eight	eight	CD
cord-324094-23kzr8rq	116	21	distinct	distinct	JJ
cord-324094-23kzr8rq	116	22	regions	region	NNS
cord-324094-23kzr8rq	116	23	on	on	IN
cord-324094-23kzr8rq	116	24	the	the	DT
cord-324094-23kzr8rq	116	25	target	target	NN
cord-324094-23kzr8rq	116	26	sequence	sequence	NN
cord-324094-23kzr8rq	116	27	.	.	.
cord-324094-23kzr8rq	117	1	The	the	DT
cord-324094-23kzr8rq	117	2	two	two	CD
cord-324094-23kzr8rq	117	3	outer	outer	JJ
cord-324094-23kzr8rq	117	4	primers	primer	NNS
cord-324094-23kzr8rq	117	5	were	be	VBD
cord-324094-23kzr8rq	117	6	described	describe	VBN
cord-324094-23kzr8rq	117	7	as	as	IN
cord-324094-23kzr8rq	117	8	forward	forward	RB
cord-324094-23kzr8rq	117	9	outer	outer	JJ
cord-324094-23kzr8rq	117	10	primer	primer	NN
cord-324094-23kzr8rq	117	11	(	(	-LRB-
cord-324094-23kzr8rq	117	12	F3	F3	NNP
cord-324094-23kzr8rq	117	13	)	)	-RRB-
cord-324094-23kzr8rq	117	14	and	and	CC
cord-324094-23kzr8rq	117	15	backward	backward	JJ
cord-324094-23kzr8rq	117	16	outer	outer	JJ
cord-324094-23kzr8rq	117	17	primer	primer	NN
cord-324094-23kzr8rq	117	18	(	(	-LRB-
cord-324094-23kzr8rq	117	19	B3	b3	NN
cord-324094-23kzr8rq	117	20	)	)	-RRB-
cord-324094-23kzr8rq	117	21	and	and	CC
cord-324094-23kzr8rq	117	22	have	have	VBP
cord-324094-23kzr8rq	117	23	a	a	DT
cord-324094-23kzr8rq	117	24	role	role	NN
cord-324094-23kzr8rq	117	25	in	in	IN
cord-324094-23kzr8rq	117	26	strand	strand	NN
cord-324094-23kzr8rq	117	27	displacement	displacement	NN
cord-324094-23kzr8rq	117	28	during	during	IN
cord-324094-23kzr8rq	117	29	non	non	JJ
cord-324094-23kzr8rq	117	30	-	-	JJ
cord-324094-23kzr8rq	117	31	cyclic	cyclic	JJ
cord-324094-23kzr8rq	117	32	step	step	NN
cord-324094-23kzr8rq	117	33	only	only	RB
cord-324094-23kzr8rq	117	34	.	.	.
cord-324094-23kzr8rq	118	1	The	the	DT
cord-324094-23kzr8rq	118	2	inner	inner	JJ
cord-324094-23kzr8rq	118	3	primers	primer	NNS
cord-324094-23kzr8rq	118	4	were	be	VBD
cord-324094-23kzr8rq	118	5	described	describe	VBN
cord-324094-23kzr8rq	118	6	as	as	IN
cord-324094-23kzr8rq	118	7	forward	forward	JJ
cord-324094-23kzr8rq	118	8	inner	inner	JJ
cord-324094-23kzr8rq	118	9	primer	primer	NN
cord-324094-23kzr8rq	118	10	(	(	-LRB-
cord-324094-23kzr8rq	118	11	FIP	FIP	NNP
cord-324094-23kzr8rq	118	12	)	)	-RRB-
cord-324094-23kzr8rq	118	13	and	and	CC
cord-324094-23kzr8rq	118	14	backward	backward	JJ
cord-324094-23kzr8rq	118	15	inner	inner	JJ
cord-324094-23kzr8rq	118	16	primer	primer	NN
cord-324094-23kzr8rq	118	17	(	(	-LRB-
cord-324094-23kzr8rq	118	18	BIP	BIP	NNP
cord-324094-23kzr8rq	118	19	)	)	-RRB-
cord-324094-23kzr8rq	118	20	having	have	VBG
cord-324094-23kzr8rq	118	21	both	both	DT
cord-324094-23kzr8rq	118	22	sense	sense	NN
cord-324094-23kzr8rq	118	23	and	and	CC
cord-324094-23kzr8rq	118	24	antisense	antisense	NN
cord-324094-23kzr8rq	118	25	sequence	sequence	NN
cord-324094-23kzr8rq	118	26	in	in	IN
cord-324094-23kzr8rq	118	27	such	such	PDT
cord-324094-23kzr8rq	118	28	a	a	DT
cord-324094-23kzr8rq	118	29	way	way	NN
cord-324094-23kzr8rq	118	30	that	that	WDT
cord-324094-23kzr8rq	118	31	it	-PRON-	PRP
cord-324094-23kzr8rq	118	32	helps	help	VBZ
cord-324094-23kzr8rq	118	33	in	in	IN
cord-324094-23kzr8rq	118	34	the	the	DT
cord-324094-23kzr8rq	118	35	formation	formation	NN
cord-324094-23kzr8rq	118	36	of	of	IN
cord-324094-23kzr8rq	118	37	loop	loop	NN
cord-324094-23kzr8rq	118	38	.	.	.
cord-324094-23kzr8rq	119	1	Further	further	RB
cord-324094-23kzr8rq	119	2	,	,	,
cord-324094-23kzr8rq	119	3	two	two	CD
cord-324094-23kzr8rq	119	4	loop	loop	NN
cord-324094-23kzr8rq	119	5	primers	primer	NNS
cord-324094-23kzr8rq	119	6	viz	viz	NN
cord-324094-23kzr8rq	119	7	.	.	.
cord-324094-23kzr8rq	120	1	forward	forward	NNP
cord-324094-23kzr8rq	120	2	loop	loop	NN
cord-324094-23kzr8rq	120	3	primer	primer	NN
cord-324094-23kzr8rq	120	4	(	(	-LRB-
cord-324094-23kzr8rq	120	5	FLP	FLP	NNP
cord-324094-23kzr8rq	120	6	)	)	-RRB-
cord-324094-23kzr8rq	120	7	and	and	CC
cord-324094-23kzr8rq	120	8	backward	backward	JJ
cord-324094-23kzr8rq	120	9	loop	loop	NN
cord-324094-23kzr8rq	120	10	primer	primer	NN
cord-324094-23kzr8rq	120	11	(	(	-LRB-
cord-324094-23kzr8rq	120	12	BLP	BLP	NNP
cord-324094-23kzr8rq	120	13	)	)	-RRB-
cord-324094-23kzr8rq	120	14	were	be	VBD
cord-324094-23kzr8rq	120	15	designed	design	VBN
cord-324094-23kzr8rq	120	16	to	to	TO
cord-324094-23kzr8rq	120	17	accelerate	accelerate	VB
cord-324094-23kzr8rq	120	18	the	the	DT
cord-324094-23kzr8rq	120	19	amplifi	amplifi	NNP
cord-324094-23kzr8rq	120	20	cation	cation	NN
cord-324094-23kzr8rq	120	21	reaction	reaction	NN
cord-324094-23kzr8rq	120	22	by	by	IN
cord-324094-23kzr8rq	120	23	binding	bind	VBG
cord-324094-23kzr8rq	120	24	to	to	IN
cord-324094-23kzr8rq	120	25	additional	additional	JJ
cord-324094-23kzr8rq	120	26	sites	site	NNS
cord-324094-23kzr8rq	120	27	that	that	WDT
cord-324094-23kzr8rq	120	28	are	be	VBP
cord-324094-23kzr8rq	120	29	not	not	RB
cord-324094-23kzr8rq	120	30	accessed	access	VBN
cord-324094-23kzr8rq	120	31	by	by	IN
cord-324094-23kzr8rq	120	32	internal	internal	JJ
cord-324094-23kzr8rq	120	33	primers	primer	NNS
cord-324094-23kzr8rq	120	34	.	.	.
cord-324094-23kzr8rq	121	1	LAMP	LAMP	NNP
cord-324094-23kzr8rq	121	2	amplifi	amplifi	NNP
cord-324094-23kzr8rq	121	3	cation	cation	NN
cord-324094-23kzr8rq	121	4	can	can	MD
cord-324094-23kzr8rq	121	5	also	also	RB
cord-324094-23kzr8rq	121	6	be	be	VB
cord-324094-23kzr8rq	121	7	accomplished	accomplish	VBN
cord-324094-23kzr8rq	121	8	with	with	IN
cord-324094-23kzr8rq	121	9	the	the	DT
cord-324094-23kzr8rq	121	10	two	two	CD
cord-324094-23kzr8rq	121	11	outer	outer	JJ
cord-324094-23kzr8rq	121	12	(	(	-LRB-
cord-324094-23kzr8rq	121	13	F3	F3	NNP
cord-324094-23kzr8rq	121	14	and	and	CC
cord-324094-23kzr8rq	121	15	B3	B3	NNP
cord-324094-23kzr8rq	121	16	)	)	-RRB-
cord-324094-23kzr8rq	121	17	and	and	CC
cord-324094-23kzr8rq	121	18	two	two	CD
cord-324094-23kzr8rq	121	19	internal	internal	JJ
cord-324094-23kzr8rq	121	20	primers	primer	NNS
cord-324094-23kzr8rq	121	21	(	(	-LRB-
cord-324094-23kzr8rq	121	22	FIP	FIP	NNP
cord-324094-23kzr8rq	121	23	and	and	CC
cord-324094-23kzr8rq	121	24	BIP	BIP	NNP
cord-324094-23kzr8rq	121	25	)	)	-RRB-
cord-324094-23kzr8rq	121	26	but	but	CC
cord-324094-23kzr8rq	121	27	by	by	IN
cord-324094-23kzr8rq	121	28	using	use	VBG
cord-324094-23kzr8rq	121	29	the	the	DT
cord-324094-23kzr8rq	121	30	two	two	CD
cord-324094-23kzr8rq	121	31	loop	loop	NN
cord-324094-23kzr8rq	121	32	primers	primer	NNS
cord-324094-23kzr8rq	121	33	(	(	-LRB-
cord-324094-23kzr8rq	121	34	FLP	FLP	NNP
cord-324094-23kzr8rq	121	35	and	and	CC
cord-324094-23kzr8rq	121	36	BLP	BLP	NNP
cord-324094-23kzr8rq	121	37	)	)	-RRB-
cord-324094-23kzr8rq	121	38	,	,	,
cord-324094-23kzr8rq	121	39	the	the	DT
cord-324094-23kzr8rq	121	40	amplifi	amplifi	NNP
cord-324094-23kzr8rq	121	41	cation	cation	NN
cord-324094-23kzr8rq	121	42	is	be	VBZ
cord-324094-23kzr8rq	121	43	accelerated	accelerate	VBN
cord-324094-23kzr8rq	121	44	and	and	CC
cord-324094-23kzr8rq	121	45	thereby	thereby	RB
cord-324094-23kzr8rq	121	46	shortens	shorten	VBZ
cord-324094-23kzr8rq	121	47	amplifi	amplifi	NNP
cord-324094-23kzr8rq	121	48	cation	cation	NN
cord-324094-23kzr8rq	121	49	time	time	NN
cord-324094-23kzr8rq	121	50	by	by	IN
cord-324094-23kzr8rq	121	51	one	one	CD
cord-324094-23kzr8rq	121	52	third	third	NN
cord-324094-23kzr8rq	121	53	to	to	IN
cord-324094-23kzr8rq	121	54	one	one	CD
cord-324094-23kzr8rq	121	55	half	half	NN
cord-324094-23kzr8rq	122	1	(	(	-LRB-
cord-324094-23kzr8rq	122	2	(	(	-LRB-
cord-324094-23kzr8rq	122	3	Notomi	Notomi	NNP
cord-324094-23kzr8rq	122	4	et	et	NNP
cord-324094-23kzr8rq	122	5	al	al	NNP
cord-324094-23kzr8rq	122	6	2000	2000	CD
cord-324094-23kzr8rq	122	7	)	)	-RRB-
cord-324094-23kzr8rq	122	8	.	.	.
cord-324094-23kzr8rq	123	1	The	the	DT
cord-324094-23kzr8rq	123	2	designing	designing	NN
cord-324094-23kzr8rq	123	3	of	of	IN
cord-324094-23kzr8rq	123	4	the	the	DT
cord-324094-23kzr8rq	123	5	above	above	RB
cord-324094-23kzr8rq	123	6	mentioned	mention	VBN
cord-324094-23kzr8rq	123	7	6	6	CD
cord-324094-23kzr8rq	123	8	types	type	NNS
cord-324094-23kzr8rq	123	9	of	of	IN
cord-324094-23kzr8rq	123	10	primers	primer	NNS
cord-324094-23kzr8rq	123	11	are	be	VBP
cord-324094-23kzr8rq	123	12	based	base	VBN
cord-324094-23kzr8rq	123	13	on	on	IN
cord-324094-23kzr8rq	123	14	the	the	DT
cord-324094-23kzr8rq	123	15	following	following	JJ
cord-324094-23kzr8rq	123	16	8	8	CD
cord-324094-23kzr8rq	123	17	distinct	distinct	JJ
cord-324094-23kzr8rq	123	18	regions	region	NNS
cord-324094-23kzr8rq	123	19	of	of	IN
cord-324094-23kzr8rq	123	20	the	the	DT
cord-324094-23kzr8rq	123	21	target	target	NN
cord-324094-23kzr8rq	123	22	gene	gene	NN
cord-324094-23kzr8rq	123	23	:	:	:
cord-324094-23kzr8rq	123	24	the	the	DT
cord-324094-23kzr8rq	123	25	F3c	F3c	NNP
cord-324094-23kzr8rq	123	26	,	,	,
cord-324094-23kzr8rq	123	27	F2c	f2c	NN
cord-324094-23kzr8rq	123	28	and	and	CC
cord-324094-23kzr8rq	123	29	F1c	F1c	NNP
cord-324094-23kzr8rq	123	30	and	and	CC
cord-324094-23kzr8rq	123	31	FLP	FLP	NNP
cord-324094-23kzr8rq	123	32	regions	region	NNS
cord-324094-23kzr8rq	123	33	at	at	IN
cord-324094-23kzr8rq	123	34	the	the	DT
cord-324094-23kzr8rq	123	35	3	3	CD
cord-324094-23kzr8rq	123	36	'	'	POS
cord-324094-23kzr8rq	123	37	side	side	NN
cord-324094-23kzr8rq	123	38	and	and	CC
cord-324094-23kzr8rq	123	39	the	the	DT
cord-324094-23kzr8rq	123	40	B1	b1	NN
cord-324094-23kzr8rq	123	41	,	,	,
cord-324094-23kzr8rq	123	42	B2	b2	NN
cord-324094-23kzr8rq	123	43	,	,	,
cord-324094-23kzr8rq	123	44	B3	b3	NN
cord-324094-23kzr8rq	123	45	and	and	CC
cord-324094-23kzr8rq	123	46	BLP	BLP	NNP
cord-324094-23kzr8rq	123	47	regions	region	NNS
cord-324094-23kzr8rq	123	48	at	at	IN
cord-324094-23kzr8rq	123	49	the	the	DT
cord-324094-23kzr8rq	123	50	5	5	CD
cord-324094-23kzr8rq	123	51	'	'	POS
cord-324094-23kzr8rq	123	52	side	side	NN
cord-324094-23kzr8rq	123	53	.	.	.
cord-324094-23kzr8rq	124	1	Forward	Forward	NNP
cord-324094-23kzr8rq	124	2	Inner	Inner	NNP
cord-324094-23kzr8rq	124	3	Primer	Primer	NNP
cord-324094-23kzr8rq	124	4	(	(	-LRB-
cord-324094-23kzr8rq	124	5	FIP	FIP	NNP
cord-324094-23kzr8rq	124	6	)	)	-RRB-
cord-324094-23kzr8rq	124	7	consists	consist	VBZ
cord-324094-23kzr8rq	124	8	of	of	IN
cord-324094-23kzr8rq	124	9	the	the	DT
cord-324094-23kzr8rq	124	10	F2	F2	NNP
cord-324094-23kzr8rq	124	11	region	region	NN
cord-324094-23kzr8rq	124	12	(	(	-LRB-
cord-324094-23kzr8rq	124	13	at	at	IN
cord-324094-23kzr8rq	124	14	the	the	DT
cord-324094-23kzr8rq	124	15	3	3	CD
cord-324094-23kzr8rq	124	16	'	'	NN
cord-324094-23kzr8rq	124	17	end	end	NN
cord-324094-23kzr8rq	124	18	)	)	-RRB-
cord-324094-23kzr8rq	125	1	that	that	DT
cord-324094-23kzr8rq	125	2	is	be	VBZ
cord-324094-23kzr8rq	125	3	complementary	complementary	JJ
cord-324094-23kzr8rq	125	4	to	to	IN
cord-324094-23kzr8rq	125	5	the	the	DT
cord-324094-23kzr8rq	125	6	F2c	f2c	JJ
cord-324094-23kzr8rq	125	7	region	region	NN
cord-324094-23kzr8rq	125	8	,	,	,
cord-324094-23kzr8rq	125	9	and	and	CC
cord-324094-23kzr8rq	125	10	the	the	DT
cord-324094-23kzr8rq	125	11	same	same	JJ
cord-324094-23kzr8rq	125	12	sequence	sequence	NN
cord-324094-23kzr8rq	125	13	as	as	IN
cord-324094-23kzr8rq	125	14	the	the	DT
cord-324094-23kzr8rq	125	15	F1c	F1c	NNP
cord-324094-23kzr8rq	125	16	region	region	NN
cord-324094-23kzr8rq	125	17	at	at	IN
cord-324094-23kzr8rq	125	18	the	the	DT
cord-324094-23kzr8rq	125	19	5	5	CD
cord-324094-23kzr8rq	125	20	'	'	NN
cord-324094-23kzr8rq	125	21	end	end	NN
cord-324094-23kzr8rq	125	22	.	.	.
cord-324094-23kzr8rq	126	1	Forward	Forward	NNP
cord-324094-23kzr8rq	126	2	Outer	Outer	NNP
cord-324094-23kzr8rq	126	3	Primer	Primer	NNP
cord-324094-23kzr8rq	126	4	consists	consist	VBZ
cord-324094-23kzr8rq	126	5	of	of	IN
cord-324094-23kzr8rq	126	6	the	the	DT
cord-324094-23kzr8rq	126	7	F3	F3	NNP
cord-324094-23kzr8rq	126	8	region	region	NN
cord-324094-23kzr8rq	126	9	that	that	WDT
cord-324094-23kzr8rq	126	10	is	be	VBZ
cord-324094-23kzr8rq	126	11	complementary	complementary	JJ
cord-324094-23kzr8rq	126	12	to	to	IN
cord-324094-23kzr8rq	126	13	the	the	DT
cord-324094-23kzr8rq	126	14	F3c	F3c	NNP
cord-324094-23kzr8rq	126	15	region	region	NN
cord-324094-23kzr8rq	126	16	.	.	.
cord-324094-23kzr8rq	127	1	Backward	backward	JJ
cord-324094-23kzr8rq	127	2	inner	inner	JJ
cord-324094-23kzr8rq	127	3	primer	primer	NN
cord-324094-23kzr8rq	127	4	(	(	-LRB-
cord-324094-23kzr8rq	127	5	BIP	BIP	NNP
cord-324094-23kzr8rq	127	6	)	)	-RRB-
cord-324094-23kzr8rq	127	7	consists	consist	VBZ
cord-324094-23kzr8rq	127	8	of	of	IN
cord-324094-23kzr8rq	127	9	the	the	DT
cord-324094-23kzr8rq	127	10	B2	b2	NN
cord-324094-23kzr8rq	127	11	region	region	NN
cord-324094-23kzr8rq	127	12	(	(	-LRB-
cord-324094-23kzr8rq	127	13	at	at	IN
cord-324094-23kzr8rq	127	14	the	the	DT
cord-324094-23kzr8rq	127	15	3	3	CD
cord-324094-23kzr8rq	127	16	'	'	NN
cord-324094-23kzr8rq	127	17	end	end	NN
cord-324094-23kzr8rq	127	18	)	)	-RRB-
cord-324094-23kzr8rq	128	1	that	that	DT
cord-324094-23kzr8rq	128	2	is	be	VBZ
cord-324094-23kzr8rq	128	3	complementary	complementary	JJ
cord-324094-23kzr8rq	128	4	to	to	IN
cord-324094-23kzr8rq	128	5	the	the	DT
cord-324094-23kzr8rq	128	6	B2c	B2c	NNP
cord-324094-23kzr8rq	128	7	region	region	NN
cord-324094-23kzr8rq	128	8	,	,	,
cord-324094-23kzr8rq	128	9	and	and	CC
cord-324094-23kzr8rq	128	10	the	the	DT
cord-324094-23kzr8rq	128	11	same	same	JJ
cord-324094-23kzr8rq	128	12	sequence	sequence	NN
cord-324094-23kzr8rq	128	13	as	as	IN
cord-324094-23kzr8rq	128	14	the	the	DT
cord-324094-23kzr8rq	128	15	B1c	B1c	NNP
cord-324094-23kzr8rq	128	16	region	region	NN
cord-324094-23kzr8rq	128	17	at	at	IN
cord-324094-23kzr8rq	128	18	the	the	DT
cord-324094-23kzr8rq	128	19	5	5	CD
cord-324094-23kzr8rq	128	20	'	'	NN
cord-324094-23kzr8rq	128	21	end	end	NN
cord-324094-23kzr8rq	128	22	.	.	.
cord-324094-23kzr8rq	129	1	Backward	Backward	NNP
cord-324094-23kzr8rq	129	2	Outer	Outer	NNP
cord-324094-23kzr8rq	129	3	Primer	Primer	NNP
cord-324094-23kzr8rq	129	4	consists	consist	VBZ
cord-324094-23kzr8rq	129	5	of	of	IN
cord-324094-23kzr8rq	129	6	the	the	DT
cord-324094-23kzr8rq	129	7	B3	b3	NN
cord-324094-23kzr8rq	129	8	region	region	NN
cord-324094-23kzr8rq	129	9	that	that	WDT
cord-324094-23kzr8rq	129	10	is	be	VBZ
cord-324094-23kzr8rq	129	11	complementary	complementary	JJ
cord-324094-23kzr8rq	129	12	to	to	IN
cord-324094-23kzr8rq	129	13	the	the	DT
cord-324094-23kzr8rq	129	14	B3c	b3c	NN
cord-324094-23kzr8rq	129	15	region	region	NN
cord-324094-23kzr8rq	129	16	.	.	.
cord-324094-23kzr8rq	130	1	FIP	FIP	NNP
cord-324094-23kzr8rq	130	2	consists	consist	VBZ
cord-324094-23kzr8rq	130	3	of	of	IN
cord-324094-23kzr8rq	130	4	a	a	DT
cord-324094-23kzr8rq	130	5	complementary	complementary	JJ
cord-324094-23kzr8rq	130	6	sequence	sequence	NN
cord-324094-23kzr8rq	130	7	of	of	IN
cord-324094-23kzr8rq	130	8	F1	f1	NN
cord-324094-23kzr8rq	130	9	and	and	CC
cord-324094-23kzr8rq	130	10	a	a	DT
cord-324094-23kzr8rq	130	11	sense	sense	NN
cord-324094-23kzr8rq	130	12	sequence	sequence	NN
cord-324094-23kzr8rq	130	13	of	of	IN
cord-324094-23kzr8rq	130	14	F2	f2	NN
cord-324094-23kzr8rq	130	15	.	.	.
cord-324094-23kzr8rq	131	1	BIP	BIP	NNP
cord-324094-23kzr8rq	131	2	consists	consist	VBZ
cord-324094-23kzr8rq	131	3	of	of	IN
cord-324094-23kzr8rq	131	4	a	a	DT
cord-324094-23kzr8rq	131	5	complementary	complementary	JJ
cord-324094-23kzr8rq	131	6	sequence	sequence	NN
cord-324094-23kzr8rq	131	7	of	of	IN
cord-324094-23kzr8rq	131	8	B1	b1	NN
cord-324094-23kzr8rq	131	9	and	and	CC
cord-324094-23kzr8rq	131	10	a	a	DT
cord-324094-23kzr8rq	131	11	sense	sense	NN
cord-324094-23kzr8rq	131	12	sequence	sequence	NN
cord-324094-23kzr8rq	131	13	of	of	IN
cord-324094-23kzr8rq	131	14	B2	b2	NN
cord-324094-23kzr8rq	131	15	(	(	-LRB-
cord-324094-23kzr8rq	131	16	fi	fi	NNP
cord-324094-23kzr8rq	131	17	gure	gure	NNP
cord-324094-23kzr8rq	131	18	3	3	CD
cord-324094-23kzr8rq	131	19	)	)	-RRB-
cord-324094-23kzr8rq	131	20	.	.	.
cord-324094-23kzr8rq	132	1	FIP	FIP	NNP
cord-324094-23kzr8rq	132	2	and	and	CC
cord-324094-23kzr8rq	132	3	BIP	BIP	NNP
cord-324094-23kzr8rq	132	4	were	be	VBD
cord-324094-23kzr8rq	132	5	high	high	JJ
cord-324094-23kzr8rq	132	6	performance	performance	NN
cord-324094-23kzr8rq	132	7	liquid	liquid	NN
cord-324094-23kzr8rq	132	8	chromatography	chromatography	NN
cord-324094-23kzr8rq	132	9	(	(	-LRB-
cord-324094-23kzr8rq	132	10	HPLC	HPLC	NNP
cord-324094-23kzr8rq	132	11	)	)	-RRB-
cord-324094-23kzr8rq	132	12	purifi	purifi	NNP
cord-324094-23kzr8rq	132	13	ed	ed	NNP
cord-324094-23kzr8rq	132	14	primers	primer	NNS
cord-324094-23kzr8rq	132	15	.	.	.
cord-324094-23kzr8rq	133	1	The	the	DT
cord-324094-23kzr8rq	133	2	FLP	FLP	NNP
cord-324094-23kzr8rq	133	3	and	and	CC
cord-324094-23kzr8rq	133	4	BLP	BLP	NNP
cord-324094-23kzr8rq	133	5	primers	primer	NNS
cord-324094-23kzr8rq	133	6	were	be	VBD
cord-324094-23kzr8rq	133	7	composed	compose	VBN
cord-324094-23kzr8rq	133	8	of	of	IN
cord-324094-23kzr8rq	133	9	the	the	DT
cord-324094-23kzr8rq	133	10	sequences	sequence	NNS
cord-324094-23kzr8rq	133	11	that	that	WDT
cord-324094-23kzr8rq	133	12	are	be	VBP
cord-324094-23kzr8rq	133	13	complementary	complementary	JJ
cord-324094-23kzr8rq	133	14	to	to	IN
cord-324094-23kzr8rq	133	15	the	the	DT
cord-324094-23kzr8rq	133	16	sequence	sequence	NN
cord-324094-23kzr8rq	133	17	between	between	IN
cord-324094-23kzr8rq	133	18	F1	F1	NNP
cord-324094-23kzr8rq	133	19	and	and	CC
cord-324094-23kzr8rq	133	20	F2	f2	NN
cord-324094-23kzr8rq	133	21	and	and	CC
cord-324094-23kzr8rq	133	22	B1	b1	NN
cord-324094-23kzr8rq	133	23	and	and	CC
cord-324094-23kzr8rq	133	24	B2	b2	NN
cord-324094-23kzr8rq	133	25	regions	region	NNS
cord-324094-23kzr8rq	133	26	respectively	respectively	RB
cord-324094-23kzr8rq	133	27	(	(	-LRB-
cord-324094-23kzr8rq	133	28	(	(	-LRB-
cord-324094-23kzr8rq	133	29	Notomi	Notomi	NNP
cord-324094-23kzr8rq	133	30	et	et	NNP
cord-324094-23kzr8rq	133	31	al	al	NNP
cord-324094-23kzr8rq	133	32	2000	2000	CD
cord-324094-23kzr8rq	133	33	)	)	-RRB-
cord-324094-23kzr8rq	133	34	.	.	.
cord-324094-23kzr8rq	134	1	The	the	DT
cord-324094-23kzr8rq	134	2	chemistry	chemistry	NN
cord-324094-23kzr8rq	134	3	of	of	IN
cord-324094-23kzr8rq	134	4	LAMP	LAMP	NNP
cord-324094-23kzr8rq	134	5	amplifi	amplifi	NNP
cord-324094-23kzr8rq	134	6	cation	cation	NN
cord-324094-23kzr8rq	134	7	is	be	VBZ
cord-324094-23kzr8rq	134	8	based	base	VBN
cord-324094-23kzr8rq	134	9	on	on	IN
cord-324094-23kzr8rq	134	10	the	the	DT
cord-324094-23kzr8rq	134	11	principle	principle	NN
cord-324094-23kzr8rq	134	12	of	of	IN
cord-324094-23kzr8rq	134	13	auto	auto	NN
cord-324094-23kzr8rq	134	14	cyclic	cyclic	JJ
cord-324094-23kzr8rq	134	15	strand	strand	NN
cord-324094-23kzr8rq	134	16	displacement	displacement	NN
cord-324094-23kzr8rq	134	17	reaction	reaction	NN
cord-324094-23kzr8rq	134	18	being	be	VBG
cord-324094-23kzr8rq	134	19	performed	perform	VBN
cord-324094-23kzr8rq	134	20	at	at	IN
cord-324094-23kzr8rq	134	21	a	a	DT
cord-324094-23kzr8rq	134	22	constant	constant	JJ
cord-324094-23kzr8rq	134	23	temperature	temperature	NN
cord-324094-23kzr8rq	134	24	.	.	.
cord-324094-23kzr8rq	135	1	This	this	DT
cord-324094-23kzr8rq	135	2	method	method	NN
cord-324094-23kzr8rq	135	3	employs	employ	VBZ
cord-324094-23kzr8rq	135	4	a	a	DT
cord-324094-23kzr8rq	135	5	DNA	dna	NN
cord-324094-23kzr8rq	135	6	polymerase	polymerase	NN
cord-324094-23kzr8rq	135	7	and	and	CC
cord-324094-23kzr8rq	135	8	a	a	DT
cord-324094-23kzr8rq	135	9	set	set	NN
cord-324094-23kzr8rq	135	10	of	of	IN
cord-324094-23kzr8rq	135	11	six	six	CD
cord-324094-23kzr8rq	135	12	specially	specially	RB
cord-324094-23kzr8rq	135	13	designed	design	VBN
cord-324094-23kzr8rq	135	14	primers	primer	NNS
cord-324094-23kzr8rq	135	15	that	that	WDT
cord-324094-23kzr8rq	135	16	recognize	recognize	VBP
cord-324094-23kzr8rq	135	17	a	a	DT
cord-324094-23kzr8rq	135	18	total	total	NN
cord-324094-23kzr8rq	135	19	of	of	IN
cord-324094-23kzr8rq	135	20	eight	eight	CD
cord-324094-23kzr8rq	135	21	distinct	distinct	JJ
cord-324094-23kzr8rq	135	22	sequences	sequence	NNS
cord-324094-23kzr8rq	135	23	on	on	IN
cord-324094-23kzr8rq	135	24	the	the	DT
cord-324094-23kzr8rq	135	25	target	target	NN
cord-324094-23kzr8rq	135	26	DNA	dna	NN
cord-324094-23kzr8rq	135	27	.	.	.
cord-324094-23kzr8rq	136	1	There	there	EX
cord-324094-23kzr8rq	136	2	are	be	VBP
cord-324094-23kzr8rq	136	3	two	two	CD
cord-324094-23kzr8rq	136	4	steps	step	NNS
cord-324094-23kzr8rq	136	5	of	of	IN
cord-324094-23kzr8rq	136	6	LAMP	LAMP	NNP
cord-324094-23kzr8rq	136	7	amplifi	amplifi	NNP
cord-324094-23kzr8rq	136	8	cation	cation	NN
cord-324094-23kzr8rq	136	9	comprising	comprise	VBG
cord-324094-23kzr8rq	136	10	non	non	JJ
cord-324094-23kzr8rq	136	11	-	-	JJ
cord-324094-23kzr8rq	136	12	cyclic	cyclic	JJ
cord-324094-23kzr8rq	136	13	and	and	CC
cord-324094-23kzr8rq	136	14	cyclic	cyclic	JJ
cord-324094-23kzr8rq	136	15	steps	step	NNS
cord-324094-23kzr8rq	136	16	.	.	.
cord-324094-23kzr8rq	137	1	In	in	IN
cord-324094-23kzr8rq	137	2	the	the	DT
cord-324094-23kzr8rq	137	3	non	non	JJ
cord-324094-23kzr8rq	137	4	-	-	JJ
cord-324094-23kzr8rq	137	5	cyclic	cyclic	JJ
cord-324094-23kzr8rq	137	6	step	step	NN
cord-324094-23kzr8rq	137	7	,	,	,
cord-324094-23kzr8rq	137	8	there	there	EX
cord-324094-23kzr8rq	137	9	is	be	VBZ
cord-324094-23kzr8rq	137	10	the	the	DT
cord-324094-23kzr8rq	137	11	formation	formation	NN
cord-324094-23kzr8rq	137	12	of	of	IN
cord-324094-23kzr8rq	137	13	stem	stem	NN
cord-324094-23kzr8rq	137	14	loop	loop	NN
cord-324094-23kzr8rq	137	15	DNA	dna	NN
cord-324094-23kzr8rq	137	16	with	with	IN
cord-324094-23kzr8rq	137	17	stem	stem	NN
cord-324094-23kzr8rq	137	18	-	-	HYPH
cord-324094-23kzr8rq	137	19	loops	loop	NNS
cord-324094-23kzr8rq	137	20	at	at	IN
cord-324094-23kzr8rq	137	21	each	each	DT
cord-324094-23kzr8rq	137	22	end	end	NN
cord-324094-23kzr8rq	137	23	that	that	WDT
cord-324094-23kzr8rq	137	24	serves	serve	VBZ
cord-324094-23kzr8rq	137	25	as	as	IN
cord-324094-23kzr8rq	137	26	the	the	DT
cord-324094-23kzr8rq	137	27	starting	starting	NN
cord-324094-23kzr8rq	137	28	structure	structure	NN
cord-324094-23kzr8rq	137	29	for	for	IN
cord-324094-23kzr8rq	137	30	the	the	DT
cord-324094-23kzr8rq	137	31	amplifi	amplifi	NNP
cord-324094-23kzr8rq	137	32	cation	cation	NN
cord-324094-23kzr8rq	137	33	by	by	IN
cord-324094-23kzr8rq	137	34	LAMP	LAMP	NNP
cord-324094-23kzr8rq	137	35	cycling	cycling	NN
cord-324094-23kzr8rq	137	36	.	.	.
cord-324094-23kzr8rq	138	1	As	as	IN
cord-324094-23kzr8rq	138	2	double	double	JJ
cord-324094-23kzr8rq	138	3	stranded	strand	VBN
cord-324094-23kzr8rq	138	4	DNA	dna	NN
cord-324094-23kzr8rq	138	5	is	be	VBZ
cord-324094-23kzr8rq	138	6	in	in	IN
cord-324094-23kzr8rq	138	7	the	the	DT
cord-324094-23kzr8rq	138	8	condition	condition	NN
cord-324094-23kzr8rq	138	9	of	of	IN
cord-324094-23kzr8rq	138	10	dynamic	dynamic	JJ
cord-324094-23kzr8rq	138	11	equilibrium	equilibrium	NN
cord-324094-23kzr8rq	138	12	at	at	IN
cord-324094-23kzr8rq	138	13	the	the	DT
cord-324094-23kzr8rq	138	14	temperature	temperature	NN
cord-324094-23kzr8rq	138	15	around	around	IN
cord-324094-23kzr8rq	138	16	65	65	CD
cord-324094-23kzr8rq	138	17	°	°	NN
cord-324094-23kzr8rq	138	18	C	c	NN
cord-324094-23kzr8rq	138	19	,	,	,
cord-324094-23kzr8rq	138	20	one	one	CD
cord-324094-23kzr8rq	138	21	of	of	IN
cord-324094-23kzr8rq	138	22	the	the	DT
cord-324094-23kzr8rq	138	23	LAMP	LAMP	NNP
cord-324094-23kzr8rq	138	24	primers	primer	NNS
cord-324094-23kzr8rq	138	25	can	can	MD
cord-324094-23kzr8rq	138	26	anneal	anneal	VB
cord-324094-23kzr8rq	138	27	to	to	IN
cord-324094-23kzr8rq	138	28	the	the	DT
cord-324094-23kzr8rq	138	29	complimentary	complimentary	JJ
cord-324094-23kzr8rq	138	30	sequence	sequence	NN
cord-324094-23kzr8rq	138	31	of	of	IN
cord-324094-23kzr8rq	138	32	double	double	JJ
cord-324094-23kzr8rq	138	33	stranded	strand	VBN
cord-324094-23kzr8rq	138	34	target	target	NN
cord-324094-23kzr8rq	138	35	DNA	dna	NN
cord-324094-23kzr8rq	138	36	,	,	,
cord-324094-23kzr8rq	138	37	then	then	RB
cord-324094-23kzr8rq	138	38	initiates	initiate	VBZ
cord-324094-23kzr8rq	138	39	DNA	dna	NN
cord-324094-23kzr8rq	138	40	synthesis	synthesis	NN
cord-324094-23kzr8rq	138	41	using	use	VBG
cord-324094-23kzr8rq	138	42	the	the	DT
cord-324094-23kzr8rq	138	43	DNA	dna	NN
cord-324094-23kzr8rq	138	44	polymerase	polymerase	NN
cord-324094-23kzr8rq	138	45	with	with	IN
cord-324094-23kzr8rq	138	46	strand	strand	NN
cord-324094-23kzr8rq	138	47	displacement	displacement	NN
cord-324094-23kzr8rq	138	48	activity	activity	NN
cord-324094-23kzr8rq	138	49	,	,	,
cord-324094-23kzr8rq	138	50	displacing	displace	VBG
cord-324094-23kzr8rq	138	51	and	and	CC
cord-324094-23kzr8rq	138	52	releasing	release	VBG
cord-324094-23kzr8rq	138	53	a	a	DT
cord-324094-23kzr8rq	138	54	single	single	JJ
cord-324094-23kzr8rq	138	55	stranded	strand	VBN
cord-324094-23kzr8rq	138	56	DNA	dna	NN
cord-324094-23kzr8rq	138	57	(	(	-LRB-
cord-324094-23kzr8rq	138	58	(	(	-LRB-
cord-324094-23kzr8rq	138	59	Notomi	Notomi	NNP
cord-324094-23kzr8rq	138	60	et	et	NNP
cord-324094-23kzr8rq	138	61	al	al	NNP
cord-324094-23kzr8rq	138	62	2000	2000	CD
cord-324094-23kzr8rq	138	63	;	;	:
cord-324094-23kzr8rq	138	64	Ushikubo	Ushikubo	NNP
cord-324094-23kzr8rq	138	65	2004	2004	CD
cord-324094-23kzr8rq	138	66	)	)	-RRB-
cord-324094-23kzr8rq	138	67	.	.	.
cord-324094-23kzr8rq	139	1	With	with	IN
cord-324094-23kzr8rq	139	2	the	the	DT
cord-324094-23kzr8rq	139	3	LAMP	LAMP	NNP
cord-324094-23kzr8rq	139	4	method	method	NN
cord-324094-23kzr8rq	139	5	,	,	,
cord-324094-23kzr8rq	139	6	unlike	unlike	IN
cord-324094-23kzr8rq	139	7	with	with	IN
cord-324094-23kzr8rq	139	8	PCR	PCR	NNP
cord-324094-23kzr8rq	139	9	,	,	,
cord-324094-23kzr8rq	139	10	there	there	EX
cord-324094-23kzr8rq	139	11	is	be	VBZ
cord-324094-23kzr8rq	139	12	no	no	DT
cord-324094-23kzr8rq	139	13	need	need	NN
cord-324094-23kzr8rq	139	14	for	for	IN
cord-324094-23kzr8rq	139	15	heat	heat	NN
cord-324094-23kzr8rq	139	16	denaturation	denaturation	NN
cord-324094-23kzr8rq	139	17	of	of	IN
cord-324094-23kzr8rq	139	18	the	the	DT
cord-324094-23kzr8rq	139	19	double	double	JJ
cord-324094-23kzr8rq	139	20	stranded	strand	VBN
cord-324094-23kzr8rq	139	21	DNA	dna	NN
cord-324094-23kzr8rq	139	22	into	into	IN
cord-324094-23kzr8rq	139	23	a	a	DT
cord-324094-23kzr8rq	139	24	single	single	JJ
cord-324094-23kzr8rq	139	25	strand	strand	NN
cord-324094-23kzr8rq	139	26	.	.	.
cord-324094-23kzr8rq	140	1	Through	through	IN
cord-324094-23kzr8rq	140	2	the	the	DT
cord-324094-23kzr8rq	140	3	activity	activity	NN
cord-324094-23kzr8rq	140	4	of	of	IN
cord-324094-23kzr8rq	140	5	DNA	dna	NN
cord-324094-23kzr8rq	140	6	polymerase	polymerase	NN
cord-324094-23kzr8rq	140	7	with	with	IN
cord-324094-23kzr8rq	140	8	strand	strand	NN
cord-324094-23kzr8rq	140	9	displacement	displacement	NN
cord-324094-23kzr8rq	140	10	activity	activity	NN
cord-324094-23kzr8rq	140	11	,	,	,
cord-324094-23kzr8rq	140	12	a	a	DT
cord-324094-23kzr8rq	140	13	DNA	dna	NN
cord-324094-23kzr8rq	140	14	strand	strand	NN
cord-324094-23kzr8rq	140	15	complementary	complementary	JJ
cord-324094-23kzr8rq	140	16	to	to	IN
cord-324094-23kzr8rq	140	17	the	the	DT
cord-324094-23kzr8rq	140	18	template	template	NN
cord-324094-23kzr8rq	140	19	DNA	dna	NN
cord-324094-23kzr8rq	140	20	is	be	VBZ
cord-324094-23kzr8rq	140	21	synthesized	synthesize	VBN
cord-324094-23kzr8rq	140	22	,	,	,
cord-324094-23kzr8rq	140	23	starting	start	VBG
cord-324094-23kzr8rq	140	24	from	from	IN
cord-324094-23kzr8rq	140	25	the	the	DT
cord-324094-23kzr8rq	140	26	3	3	CD
cord-324094-23kzr8rq	140	27	'	'	NN
cord-324094-23kzr8rq	140	28	end	end	NN
cord-324094-23kzr8rq	140	29	of	of	IN
cord-324094-23kzr8rq	140	30	the	the	DT
cord-324094-23kzr8rq	140	31	F2	F2	NNP
cord-324094-23kzr8rq	140	32	region	region	NN
cord-324094-23kzr8rq	140	33	of	of	IN
cord-324094-23kzr8rq	140	34	the	the	DT
cord-324094-23kzr8rq	140	35	FIP	FIP	NNP
cord-324094-23kzr8rq	140	36	.	.	.
cord-324094-23kzr8rq	141	1	The	the	DT
cord-324094-23kzr8rq	141	2	F3	F3	NNP
cord-324094-23kzr8rq	141	3	Primer	Primer	NNP
cord-324094-23kzr8rq	141	4	anneals	anneal	VBZ
cord-324094-23kzr8rq	141	5	to	to	IN
cord-324094-23kzr8rq	141	6	the	the	DT
cord-324094-23kzr8rq	141	7	F3c	F3c	NNP
cord-324094-23kzr8rq	141	8	region	region	NN
cord-324094-23kzr8rq	141	9	,	,	,
cord-324094-23kzr8rq	141	10	outside	outside	RB
cord-324094-23kzr8rq	141	11	of	of	IN
cord-324094-23kzr8rq	141	12	FIP	FIP	NNP
cord-324094-23kzr8rq	141	13	,	,	,
cord-324094-23kzr8rq	141	14	on	on	IN
cord-324094-23kzr8rq	141	15	the	the	DT
cord-324094-23kzr8rq	141	16	target	target	NN
cord-324094-23kzr8rq	141	17	DNA	dna	NN
cord-324094-23kzr8rq	141	18	and	and	CC
cord-324094-23kzr8rq	141	19	initiates	initiate	VBZ
cord-324094-23kzr8rq	141	20	strand	strand	NN
cord-324094-23kzr8rq	141	21	displacement	displacement	NN
cord-324094-23kzr8rq	141	22	DNA	dna	NN
cord-324094-23kzr8rq	141	23	synthesis	synthesis	NN
cord-324094-23kzr8rq	141	24	,	,	,
cord-324094-23kzr8rq	141	25	releasing	release	VBG
cord-324094-23kzr8rq	141	26	the	the	DT
cord-324094-23kzr8rq	141	27	FIP	FIP	NNP
cord-324094-23kzr8rq	141	28	-	-	HYPH
cord-324094-23kzr8rq	141	29	linked	link	VBN
cord-324094-23kzr8rq	141	30	complementary	complementary	JJ
cord-324094-23kzr8rq	141	31	strand	strand	NN
cord-324094-23kzr8rq	141	32	.	.	.
cord-324094-23kzr8rq	142	1	A	a	DT
cord-324094-23kzr8rq	142	2	double	double	JJ
cord-324094-23kzr8rq	142	3	strand	strand	NN
cord-324094-23kzr8rq	142	4	is	be	VBZ
cord-324094-23kzr8rq	142	5	formed	form	VBN
cord-324094-23kzr8rq	142	6	from	from	IN
cord-324094-23kzr8rq	142	7	the	the	DT
cord-324094-23kzr8rq	142	8	DNA	dna	NN
cord-324094-23kzr8rq	142	9	strand	strand	NN
cord-324094-23kzr8rq	142	10	synthesized	synthesize	VBN
cord-324094-23kzr8rq	142	11	from	from	IN
cord-324094-23kzr8rq	142	12	the	the	DT
cord-324094-23kzr8rq	142	13	F3	F3	NNP
cord-324094-23kzr8rq	142	14	Primer	Primer	NNP
cord-324094-23kzr8rq	142	15	and	and	CC
cord-324094-23kzr8rq	142	16	the	the	DT
cord-324094-23kzr8rq	142	17	template	template	NN
cord-324094-23kzr8rq	142	18	DNA	dna	NN
cord-324094-23kzr8rq	142	19	strand	strand	NN
cord-324094-23kzr8rq	142	20	.	.	.
cord-324094-23kzr8rq	143	1	The	the	DT
cord-324094-23kzr8rq	143	2	FIP	FIP	NNP
cord-324094-23kzr8rq	143	3	-	-	HYPH
cord-324094-23kzr8rq	143	4	linked	link	VBN
cord-324094-23kzr8rq	143	5	complementary	complementary	JJ
cord-324094-23kzr8rq	143	6	strand	strand	NN
cord-324094-23kzr8rq	143	7	is	be	VBZ
cord-324094-23kzr8rq	143	8	released	release	VBN
cord-324094-23kzr8rq	143	9	as	as	IN
cord-324094-23kzr8rq	143	10	a	a	DT
cord-324094-23kzr8rq	143	11	single	single	JJ
cord-324094-23kzr8rq	143	12	strand	strand	NN
cord-324094-23kzr8rq	143	13	because	because	IN
cord-324094-23kzr8rq	143	14	of	of	IN
cord-324094-23kzr8rq	143	15	the	the	DT
cord-324094-23kzr8rq	143	16	displacement	displacement	NN
cord-324094-23kzr8rq	143	17	by	by	IN
cord-324094-23kzr8rq	143	18	the	the	DT
cord-324094-23kzr8rq	143	19	DNA	dna	NN
cord-324094-23kzr8rq	143	20	strand	strand	NN
cord-324094-23kzr8rq	143	21	synthesized	synthesize	VBN
cord-324094-23kzr8rq	143	22	from	from	IN
cord-324094-23kzr8rq	143	23	the	the	DT
cord-324094-23kzr8rq	143	24	F3	F3	NNP
cord-324094-23kzr8rq	143	25	Primer	Primer	NNP
cord-324094-23kzr8rq	143	26	.	.	.
cord-324094-23kzr8rq	144	1	Then	then	RB
cord-324094-23kzr8rq	144	2	,	,	,
cord-324094-23kzr8rq	144	3	this	this	DT
cord-324094-23kzr8rq	144	4	released	release	VBN
cord-324094-23kzr8rq	144	5	single	single	JJ
cord-324094-23kzr8rq	144	6	strand	strand	NN
cord-324094-23kzr8rq	144	7	forms	form	VBZ
cord-324094-23kzr8rq	144	8	a	a	DT
cord-324094-23kzr8rq	144	9	stem	stem	NN
cord-324094-23kzr8rq	144	10	-	-	HYPH
cord-324094-23kzr8rq	144	11	loop	loop	NN
cord-324094-23kzr8rq	144	12	structure	structure	NN
cord-324094-23kzr8rq	144	13	at	at	IN
cord-324094-23kzr8rq	144	14	the	the	DT
cord-324094-23kzr8rq	144	15	5	5	CD
cord-324094-23kzr8rq	144	16	'	'	NN
cord-324094-23kzr8rq	144	17	end	end	NN
cord-324094-23kzr8rq	144	18	because	because	IN
cord-324094-23kzr8rq	144	19	of	of	IN
cord-324094-23kzr8rq	144	20	the	the	DT
cord-324094-23kzr8rq	144	21	complementary	complementary	JJ
cord-324094-23kzr8rq	144	22	F1c	F1c	NNP
cord-324094-23kzr8rq	144	23	and	and	CC
cord-324094-23kzr8rq	144	24	F1	f1	NN
cord-324094-23kzr8rq	144	25	regions	region	NNS
cord-324094-23kzr8rq	144	26	.	.	.
cord-324094-23kzr8rq	145	1	This	this	DT
cord-324094-23kzr8rq	145	2	single	single	JJ
cord-324094-23kzr8rq	145	3	strand	strand	NN
cord-324094-23kzr8rq	145	4	DNA	dna	NN
cord-324094-23kzr8rq	145	5	in	in	IN
cord-324094-23kzr8rq	145	6	turn	turn	NN
cord-324094-23kzr8rq	145	7	serves	serve	VBZ
cord-324094-23kzr8rq	145	8	as	as	IN
cord-324094-23kzr8rq	145	9	a	a	DT
cord-324094-23kzr8rq	145	10	template	template	NN
cord-324094-23kzr8rq	145	11	for	for	IN
cord-324094-23kzr8rq	145	12	BIP	BIP	NNP
cord-324094-23kzr8rq	145	13	-	-	HYPH
cord-324094-23kzr8rq	145	14	initiated	initiate	VBN
cord-324094-23kzr8rq	145	15	DNA	dna	NN
cord-324094-23kzr8rq	145	16	synthesis	synthesis	NN
cord-324094-23kzr8rq	145	17	and	and	CC
cord-324094-23kzr8rq	145	18	subsequent	subsequent	JJ
cord-324094-23kzr8rq	145	19	B3-primed	b3-prime	VBN
cord-324094-23kzr8rq	145	20	strand	strand	NN
cord-324094-23kzr8rq	145	21	displacement	displacement	NN
cord-324094-23kzr8rq	145	22	DNA	dna	NN
cord-324094-23kzr8rq	145	23	synthesis	synthesis	NN
cord-324094-23kzr8rq	145	24	.	.	.
cord-324094-23kzr8rq	146	1	The	the	DT
cord-324094-23kzr8rq	146	2	BIP	BIP	NNP
cord-324094-23kzr8rq	146	3	anneals	anneal	NNS
cord-324094-23kzr8rq	146	4	to	to	IN
cord-324094-23kzr8rq	146	5	the	the	DT
cord-324094-23kzr8rq	146	6	DNA	dna	NN
cord-324094-23kzr8rq	146	7	strand	strand	NN
cord-324094-23kzr8rq	146	8	produced	produce	VBN
cord-324094-23kzr8rq	146	9	by	by	IN
cord-324094-23kzr8rq	146	10	the	the	DT
cord-324094-23kzr8rq	146	11	above	above	JJ
cord-324094-23kzr8rq	146	12	step	step	NN
cord-324094-23kzr8rq	146	13	.	.	.
cord-324094-23kzr8rq	147	1	Starting	start	VBG
cord-324094-23kzr8rq	147	2	from	from	IN
cord-324094-23kzr8rq	147	3	the	the	DT
cord-324094-23kzr8rq	147	4	3	3	CD
cord-324094-23kzr8rq	147	5	'	'	NN
cord-324094-23kzr8rq	147	6	end	end	NN
cord-324094-23kzr8rq	147	7	of	of	IN
cord-324094-23kzr8rq	147	8	the	the	DT
cord-324094-23kzr8rq	147	9	BIP	BIP	NNP
cord-324094-23kzr8rq	147	10	,	,	,
cord-324094-23kzr8rq	147	11	synthesis	synthesis	NN
cord-324094-23kzr8rq	147	12	of	of	IN
cord-324094-23kzr8rq	147	13	complementary	complementary	JJ
cord-324094-23kzr8rq	147	14	DNA	dna	NN
cord-324094-23kzr8rq	147	15	takes	take	VBZ
cord-324094-23kzr8rq	147	16	place	place	NN
cord-324094-23kzr8rq	147	17	.	.	.
cord-324094-23kzr8rq	148	1	Through	through	IN
cord-324094-23kzr8rq	148	2	this	this	DT
cord-324094-23kzr8rq	148	3	process	process	NN
cord-324094-23kzr8rq	148	4	,	,	,
cord-324094-23kzr8rq	148	5	the	the	DT
cord-324094-23kzr8rq	148	6	DNA	dna	NN
cord-324094-23kzr8rq	148	7	reverts	revert	VBZ
cord-324094-23kzr8rq	148	8	from	from	IN
cord-324094-23kzr8rq	148	9	a	a	DT
cord-324094-23kzr8rq	148	10	loop	loop	NN
cord-324094-23kzr8rq	148	11	structure	structure	NN
cord-324094-23kzr8rq	148	12	into	into	IN
cord-324094-23kzr8rq	148	13	a	a	DT
cord-324094-23kzr8rq	148	14	linear	linear	JJ
cord-324094-23kzr8rq	148	15	structure	structure	NN
cord-324094-23kzr8rq	148	16	.	.	.
cord-324094-23kzr8rq	149	1	The	the	DT
cord-324094-23kzr8rq	149	2	B3	B3	NNP
cord-324094-23kzr8rq	149	3	Primer	Primer	NNP
cord-324094-23kzr8rq	149	4	anneals	anneal	NNS
cord-324094-23kzr8rq	149	5	to	to	IN
cord-324094-23kzr8rq	149	6	the	the	DT
cord-324094-23kzr8rq	149	7	outside	outside	NN
cord-324094-23kzr8rq	149	8	of	of	IN
cord-324094-23kzr8rq	149	9	the	the	DT
cord-324094-23kzr8rq	149	10	BIP	BIP	NNP
cord-324094-23kzr8rq	150	1	and	and	CC
cord-324094-23kzr8rq	150	2	then	then	RB
cord-324094-23kzr8rq	150	3	,	,	,
cord-324094-23kzr8rq	150	4	through	through	IN
cord-324094-23kzr8rq	150	5	the	the	DT
cord-324094-23kzr8rq	150	6	activity	activity	NN
cord-324094-23kzr8rq	150	7	of	of	IN
cord-324094-23kzr8rq	150	8	the	the	DT
cord-324094-23kzr8rq	150	9	DNA	dna	NN
cord-324094-23kzr8rq	150	10	polymerase	polymerase	NN
cord-324094-23kzr8rq	150	11	and	and	CC
cord-324094-23kzr8rq	150	12	starting	start	VBG
cord-324094-23kzr8rq	150	13	at	at	IN
cord-324094-23kzr8rq	150	14	the	the	DT
cord-324094-23kzr8rq	150	15	3	3	CD
cord-324094-23kzr8rq	150	16	'	'	NN
cord-324094-23kzr8rq	150	17	end	end	NN
cord-324094-23kzr8rq	150	18	,	,	,
cord-324094-23kzr8rq	150	19	the	the	DT
cord-324094-23kzr8rq	150	20	DNA	dna	NN
cord-324094-23kzr8rq	150	21	synthesized	synthesize	VBN
cord-324094-23kzr8rq	150	22	from	from	IN
cord-324094-23kzr8rq	150	23	the	the	DT
cord-324094-23kzr8rq	150	24	BIP	BIP	NNP
cord-324094-23kzr8rq	150	25	is	be	VBZ
cord-324094-23kzr8rq	150	26	displaced	displace	VBN
cord-324094-23kzr8rq	150	27	and	and	CC
cord-324094-23kzr8rq	150	28	released	release	VBN
cord-324094-23kzr8rq	150	29	as	as	IN
cord-324094-23kzr8rq	150	30	a	a	DT
cord-324094-23kzr8rq	150	31	single	single	JJ
cord-324094-23kzr8rq	150	32	strand	strand	NN
cord-324094-23kzr8rq	150	33	before	before	IN
cord-324094-23kzr8rq	150	34	DNA	dna	NN
cord-324094-23kzr8rq	150	35	synthesis	synthesis	NN
cord-324094-23kzr8rq	150	36	from	from	IN
cord-324094-23kzr8rq	150	37	the	the	DT
cord-324094-23kzr8rq	150	38	B3	B3	NNP
cord-324094-23kzr8rq	150	39	Primer	Primer	NNP
cord-324094-23kzr8rq	150	40	.	.	.
cord-324094-23kzr8rq	151	1	The	the	DT
cord-324094-23kzr8rq	151	2	BIP	BIP	NNP
cord-324094-23kzr8rq	151	3	-	-	HYPH
cord-324094-23kzr8rq	151	4	linked	link	VBN
cord-324094-23kzr8rq	151	5	complementary	complementary	JJ
cord-324094-23kzr8rq	151	6	strand	strand	NN
cord-324094-23kzr8rq	151	7	displaced	displace	VBN
cord-324094-23kzr8rq	151	8	forms	form	NNS
cord-324094-23kzr8rq	151	9	a	a	DT
cord-324094-23kzr8rq	151	10	structure	structure	NN
cord-324094-23kzr8rq	151	11	with	with	IN
cord-324094-23kzr8rq	151	12	stemloops	stemloop	NNS
cord-324094-23kzr8rq	151	13	at	at	IN
cord-324094-23kzr8rq	151	14	each	each	DT
cord-324094-23kzr8rq	151	15	end	end	NN
cord-324094-23kzr8rq	151	16	,	,	,
cord-324094-23kzr8rq	151	17	which	which	WDT
cord-324094-23kzr8rq	151	18	looks	look	VBZ
cord-324094-23kzr8rq	151	19	like	like	IN
cord-324094-23kzr8rq	151	20	a	a	DT
cord-324094-23kzr8rq	151	21	dumbbell	dumbbell	JJ
cord-324094-23kzr8rq	151	22	structure	structure	NN
cord-324094-23kzr8rq	151	23	.	.	.
cord-324094-23kzr8rq	152	1	A	a	DT
cord-324094-23kzr8rq	152	2	dumbbell	dumbbell	JJ
cord-324094-23kzr8rq	152	3	-	-	HYPH
cord-324094-23kzr8rq	152	4	like	like	JJ
cord-324094-23kzr8rq	152	5	DNA	dna	NN
cord-324094-23kzr8rq	152	6	structure	structure	NN
cord-324094-23kzr8rq	152	7	is	be	VBZ
cord-324094-23kzr8rq	152	8	quickly	quickly	RB
cord-324094-23kzr8rq	152	9	converted	convert	VBN
cord-324094-23kzr8rq	152	10	into	into	IN
cord-324094-23kzr8rq	152	11	a	a	DT
cord-324094-23kzr8rq	152	12	stem	stem	NN
cord-324094-23kzr8rq	152	13	-	-	HYPH
cord-324094-23kzr8rq	152	14	loop	loop	NN
cord-324094-23kzr8rq	152	15	DNA	dna	NN
cord-324094-23kzr8rq	152	16	by	by	IN
cord-324094-23kzr8rq	152	17	self	self	NN
cord-324094-23kzr8rq	152	18	-	-	HYPH
cord-324094-23kzr8rq	152	19	primed	prime	VBN
cord-324094-23kzr8rq	152	20	DNA	dna	NN
cord-324094-23kzr8rq	152	21	synthesis	synthesis	NN
cord-324094-23kzr8rq	152	22	.	.	.
cord-324094-23kzr8rq	153	1	This	this	DT
cord-324094-23kzr8rq	153	2	structure	structure	NN
cord-324094-23kzr8rq	153	3	serves	serve	VBZ
cord-324094-23kzr8rq	153	4	as	as	IN
cord-324094-23kzr8rq	153	5	the	the	DT
cord-324094-23kzr8rq	153	6	starting	starting	NN
cord-324094-23kzr8rq	153	7	structure	structure	NN
cord-324094-23kzr8rq	153	8	for	for	IN
cord-324094-23kzr8rq	153	9	the	the	DT
cord-324094-23kzr8rq	153	10	exponential	exponential	JJ
cord-324094-23kzr8rq	153	11	amplifi	amplifi	NNP
cord-324094-23kzr8rq	153	12	cation	cation	NN
cord-324094-23kzr8rq	153	13	in	in	IN
cord-324094-23kzr8rq	153	14	cyclic	cyclic	JJ
cord-324094-23kzr8rq	153	15	manner	manner	NN
cord-324094-23kzr8rq	153	16	.	.	.
cord-324094-23kzr8rq	154	1	In	in	IN
cord-324094-23kzr8rq	154	2	subsequent	subsequent	JJ
cord-324094-23kzr8rq	154	3	LAMP	lamp	NN
cord-324094-23kzr8rq	154	4	cycling	cycle	VBG
cord-324094-23kzr8rq	154	5	one	one	CD
cord-324094-23kzr8rq	154	6	inner	inner	JJ
cord-324094-23kzr8rq	154	7	primer	primer	NN
cord-324094-23kzr8rq	154	8	hybridizes	hybridize	VBZ
cord-324094-23kzr8rq	154	9	to	to	IN
cord-324094-23kzr8rq	154	10	the	the	DT
cord-324094-23kzr8rq	154	11	loop	loop	NN
cord-324094-23kzr8rq	154	12	on	on	IN
cord-324094-23kzr8rq	154	13	the	the	DT
cord-324094-23kzr8rq	154	14	product	product	NN
cord-324094-23kzr8rq	154	15	and	and	CC
cord-324094-23kzr8rq	154	16	initiates	initiate	VBZ
cord-324094-23kzr8rq	154	17	displacement	displacement	NN
cord-324094-23kzr8rq	154	18	DNA	dna	NN
cord-324094-23kzr8rq	154	19	synthesis	synthesis	NN
cord-324094-23kzr8rq	154	20	,	,	,
cord-324094-23kzr8rq	154	21	yielding	yield	VBG
cord-324094-23kzr8rq	154	22	the	the	DT
cord-324094-23kzr8rq	154	23	original	original	JJ
cord-324094-23kzr8rq	154	24	stem	stem	NN
cord-324094-23kzr8rq	154	25	-	-	HYPH
cord-324094-23kzr8rq	154	26	loop	loop	NN
cord-324094-23kzr8rq	154	27	DNA	dna	NN
cord-324094-23kzr8rq	154	28	and	and	CC
cord-324094-23kzr8rq	154	29	a	a	DT
cord-324094-23kzr8rq	154	30	new	new	JJ
cord-324094-23kzr8rq	154	31	stem	stem	NN
cord-324094-23kzr8rq	154	32	-	-	HYPH
cord-324094-23kzr8rq	154	33	loop	loop	NN
cord-324094-23kzr8rq	154	34	DNA	dna	NN
cord-324094-23kzr8rq	154	35	with	with	IN
cord-324094-23kzr8rq	154	36	a	a	DT
cord-324094-23kzr8rq	154	37	stem	stem	NN
cord-324094-23kzr8rq	154	38	twice	twice	RB
cord-324094-23kzr8rq	154	39	as	as	RB
cord-324094-23kzr8rq	154	40	long	long	RB
cord-324094-23kzr8rq	154	41	.	.	.
cord-324094-23kzr8rq	155	1	Briefl	Briefl	NNP
cord-324094-23kzr8rq	155	2	y	y	NNP
cord-324094-23kzr8rq	155	3	,	,	,
cord-324094-23kzr8rq	155	4	FIP	FIP	NNP
cord-324094-23kzr8rq	155	5	anneals	anneal	NNS
cord-324094-23kzr8rq	155	6	to	to	IN
cord-324094-23kzr8rq	155	7	the	the	DT
cord-324094-23kzr8rq	155	8	single	single	JJ
cord-324094-23kzr8rq	155	9	stranded	strand	VBN
cord-324094-23kzr8rq	155	10	region	region	NN
cord-324094-23kzr8rq	155	11	in	in	IN
cord-324094-23kzr8rq	155	12	the	the	DT
cord-324094-23kzr8rq	155	13	stem	stem	NN
cord-324094-23kzr8rq	155	14	-	-	HYPH
cord-324094-23kzr8rq	155	15	loop	loop	NN
cord-324094-23kzr8rq	155	16	DNA	dna	NN
cord-324094-23kzr8rq	155	17	and	and	CC
cord-324094-23kzr8rq	155	18	primes	prime	NNS
cord-324094-23kzr8rq	155	19	strand	strand	NN
cord-324094-23kzr8rq	155	20	displacement	displacement	NN
cord-324094-23kzr8rq	155	21	DNA	dna	NN
cord-324094-23kzr8rq	155	22	synthesis	synthesis	NN
cord-324094-23kzr8rq	155	23	,	,	,
cord-324094-23kzr8rq	155	24	releasing	release	VBG
cord-324094-23kzr8rq	155	25	the	the	DT
cord-324094-23kzr8rq	155	26	previously	previously	RB
cord-324094-23kzr8rq	155	27	synthesized	synthesize	VBN
cord-324094-23kzr8rq	155	28	strand	strand	NN
cord-324094-23kzr8rq	155	29	.	.	.
cord-324094-23kzr8rq	156	1	This	this	DT
cord-324094-23kzr8rq	156	2	released	release	VBN
cord-324094-23kzr8rq	156	3	single	single	JJ
cord-324094-23kzr8rq	156	4	strand	strand	NN
cord-324094-23kzr8rq	156	5	forms	form	VBZ
cord-324094-23kzr8rq	156	6	a	a	DT
cord-324094-23kzr8rq	156	7	stem	stem	NN
cord-324094-23kzr8rq	156	8	-	-	HYPH
cord-324094-23kzr8rq	156	9	loop	loop	NN
cord-324094-23kzr8rq	156	10	structure	structure	NN
cord-324094-23kzr8rq	156	11	at	at	IN
cord-324094-23kzr8rq	156	12	the	the	DT
cord-324094-23kzr8rq	156	13	3	3	CD
cord-324094-23kzr8rq	156	14	'	'	NN
cord-324094-23kzr8rq	156	15	end	end	NN
cord-324094-23kzr8rq	156	16	because	because	IN
cord-324094-23kzr8rq	156	17	of	of	IN
cord-324094-23kzr8rq	156	18	complementary	complementary	JJ
cord-324094-23kzr8rq	156	19	B1c	B1c	NNP
cord-324094-23kzr8rq	156	20	and	and	CC
cord-324094-23kzr8rq	156	21	B1	b1	NN
cord-324094-23kzr8rq	156	22	regions	region	NNS
cord-324094-23kzr8rq	156	23	.	.	.
cord-324094-23kzr8rq	157	1	Then	then	RB
cord-324094-23kzr8rq	157	2	,	,	,
cord-324094-23kzr8rq	157	3	starting	start	VBG
cord-324094-23kzr8rq	157	4	from	from	IN
cord-324094-23kzr8rq	157	5	the	the	DT
cord-324094-23kzr8rq	157	6	3	3	CD
cord-324094-23kzr8rq	157	7	'	'	NN
cord-324094-23kzr8rq	157	8	end	end	NN
cord-324094-23kzr8rq	157	9	of	of	IN
cord-324094-23kzr8rq	157	10	the	the	DT
cord-324094-23kzr8rq	157	11	B1	b1	NN
cord-324094-23kzr8rq	157	12	region	region	NN
cord-324094-23kzr8rq	157	13	,	,	,
cord-324094-23kzr8rq	157	14	DNA	dna	NN
cord-324094-23kzr8rq	157	15	synthesis	synthesis	NN
cord-324094-23kzr8rq	157	16	starts	start	VBZ
cord-324094-23kzr8rq	157	17	using	use	VBG
cord-324094-23kzr8rq	157	18	self	self	NN
cord-324094-23kzr8rq	157	19	-	-	HYPH
cord-324094-23kzr8rq	157	20	structure	structure	NN
cord-324094-23kzr8rq	157	21	as	as	IN
cord-324094-23kzr8rq	157	22	a	a	DT
cord-324094-23kzr8rq	157	23	template	template	NN
cord-324094-23kzr8rq	157	24	,	,	,
cord-324094-23kzr8rq	157	25	and	and	CC
cord-324094-23kzr8rq	157	26	releases	release	VBZ
cord-324094-23kzr8rq	157	27	FIPlinked	FIPlinked	NNP
cord-324094-23kzr8rq	157	28	complementary	complementary	JJ
cord-324094-23kzr8rq	157	29	strand	strand	NN
cord-324094-23kzr8rq	157	30	.	.	.
cord-324094-23kzr8rq	158	1	The	the	DT
cord-324094-23kzr8rq	158	2	released	release	VBN
cord-324094-23kzr8rq	158	3	single	single	JJ
cord-324094-23kzr8rq	158	4	strand	strand	NN
cord-324094-23kzr8rq	158	5	then	then	RB
cord-324094-23kzr8rq	158	6	forms	form	VBZ
cord-324094-23kzr8rq	158	7	a	a	DT
cord-324094-23kzr8rq	158	8	dumbbell	dumbbell	JJ
cord-324094-23kzr8rq	158	9	-	-	HYPH
cord-324094-23kzr8rq	158	10	like	like	JJ
cord-324094-23kzr8rq	158	11	structure	structure	NN
cord-324094-23kzr8rq	158	12	as	as	IN
cord-324094-23kzr8rq	158	13	both	both	DT
cord-324094-23kzr8rq	158	14	ends	end	NNS
cord-324094-23kzr8rq	158	15	have	have	VBP
cord-324094-23kzr8rq	158	16	complementary	complementary	JJ
cord-324094-23kzr8rq	158	17	F1	F1	NNP
cord-324094-23kzr8rq	158	18	-F1c	-f1c	CD
cord-324094-23kzr8rq	158	19	and	and	CC
cord-324094-23kzr8rq	159	1	B1c	b1c	JJ
cord-324094-23kzr8rq	159	2	-B1	-B1	NNS
cord-324094-23kzr8rq	159	3	regions	region	NNS
cord-324094-23kzr8rq	159	4	,	,	,
cord-324094-23kzr8rq	159	5	respectively	respectively	RB
cord-324094-23kzr8rq	159	6	.	.	.
cord-324094-23kzr8rq	160	1	Furthermore	furthermore	RB
cord-324094-23kzr8rq	160	2	,	,	,
cord-324094-23kzr8rq	160	3	BIP	BIP	NNP
cord-324094-23kzr8rq	160	4	anneals	anneal	NNS
cord-324094-23kzr8rq	160	5	to	to	IN
cord-324094-23kzr8rq	160	6	the	the	DT
cord-324094-23kzr8rq	160	7	B2c	B2c	NNP
cord-324094-23kzr8rq	160	8	region	region	NN
cord-324094-23kzr8rq	160	9	and	and	CC
cord-324094-23kzr8rq	160	10	primes	prime	NNS
cord-324094-23kzr8rq	160	11	strand	strand	NN
cord-324094-23kzr8rq	160	12	displacement	displacement	NN
cord-324094-23kzr8rq	160	13	DNA	dna	NN
cord-324094-23kzr8rq	160	14	synthesis	synthesis	NN
cord-324094-23kzr8rq	160	15	,	,	,
cord-324094-23kzr8rq	160	16	releasing	release	VBG
cord-324094-23kzr8rq	160	17	the	the	DT
cord-324094-23kzr8rq	160	18	B1primed	B1primed	NNP
cord-324094-23kzr8rq	160	19	DNA	dna	NN
cord-324094-23kzr8rq	160	20	strand	strand	NN
cord-324094-23kzr8rq	160	21	.	.	.
cord-324094-23kzr8rq	161	1	As	as	IN
cord-324094-23kzr8rq	161	2	a	a	DT
cord-324094-23kzr8rq	161	3	result	result	NN
cord-324094-23kzr8rq	161	4	of	of	IN
cord-324094-23kzr8rq	161	5	this	this	DT
cord-324094-23kzr8rq	161	6	process	process	NN
cord-324094-23kzr8rq	161	7	,	,	,
cord-324094-23kzr8rq	161	8	various	various	JJ
cord-324094-23kzr8rq	161	9	sized	sized	JJ
cord-324094-23kzr8rq	161	10	structures	structure	NNS
cord-324094-23kzr8rq	161	11	consisting	consist	VBG
cord-324094-23kzr8rq	161	12	of	of	IN
cord-324094-23kzr8rq	161	13	alternately	alternately	RB
cord-324094-23kzr8rq	161	14	inverted	invert	VBN
cord-324094-23kzr8rq	161	15	repeats	repeat	NNS
cord-324094-23kzr8rq	161	16	of	of	IN
cord-324094-23kzr8rq	161	17	the	the	DT
cord-324094-23kzr8rq	161	18	target	target	NN
cord-324094-23kzr8rq	161	19	sequence	sequence	NN
cord-324094-23kzr8rq	161	20	on	on	IN
cord-324094-23kzr8rq	161	21	the	the	DT
cord-324094-23kzr8rq	161	22	same	same	JJ
cord-324094-23kzr8rq	161	23	strand	strand	NN
cord-324094-23kzr8rq	161	24	are	be	VBP
cord-324094-23kzr8rq	161	25	formed	form	VBN
cord-324094-23kzr8rq	161	26	.	.	.
cord-324094-23kzr8rq	162	1	The	the	DT
cord-324094-23kzr8rq	162	2	cycling	cycling	NN
cord-324094-23kzr8rq	162	3	reaction	reaction	NN
cord-324094-23kzr8rq	162	4	continues	continue	VBZ
cord-324094-23kzr8rq	162	5	with	with	IN
cord-324094-23kzr8rq	162	6	accumulation	accumulation	NN
cord-324094-23kzr8rq	162	7	of	of	IN
cord-324094-23kzr8rq	162	8	10	10	CD
cord-324094-23kzr8rq	162	9	9	9	CD
cord-324094-23kzr8rq	162	10	copies	copy	NNS
cord-324094-23kzr8rq	162	11	of	of	IN
cord-324094-23kzr8rq	162	12	target	target	NN
cord-324094-23kzr8rq	162	13	in	in	IN
cord-324094-23kzr8rq	162	14	less	less	JJR
cord-324094-23kzr8rq	162	15	than	than	IN
cord-324094-23kzr8rq	162	16	an	an	DT
cord-324094-23kzr8rq	162	17	hour	hour	NN
cord-324094-23kzr8rq	162	18	.	.	.
cord-324094-23kzr8rq	163	1	The	the	DT
cord-324094-23kzr8rq	163	2	fi	fi	FW
cord-324094-23kzr8rq	163	3	nal	nal	FW
cord-324094-23kzr8rq	163	4	products	product	NNS
cord-324094-23kzr8rq	163	5	are	be	VBP
cord-324094-23kzr8rq	163	6	stem	stem	NN
cord-324094-23kzr8rq	163	7	-	-	HYPH
cord-324094-23kzr8rq	163	8	loop	loop	NN
cord-324094-23kzr8rq	163	9	DNAs	dna	NNS
cord-324094-23kzr8rq	163	10	with	with	IN
cord-324094-23kzr8rq	163	11	several	several	JJ
cord-324094-23kzr8rq	163	12	inverted	inverted	JJ
cord-324094-23kzr8rq	163	13	repeats	repeat	NNS
cord-324094-23kzr8rq	163	14	of	of	IN
cord-324094-23kzr8rq	163	15	the	the	DT
cord-324094-23kzr8rq	163	16	target	target	NN
cord-324094-23kzr8rq	163	17	and	and	CC
cord-324094-23kzr8rq	163	18	caulifl	caulifl	VB
cord-324094-23kzr8rq	163	19	owerlike	owerlike	JJ
cord-324094-23kzr8rq	163	20	structures	structure	NNS
cord-324094-23kzr8rq	163	21	with	with	IN
cord-324094-23kzr8rq	163	22	multiple	multiple	JJ
cord-324094-23kzr8rq	163	23	loops	loop	NNS
cord-324094-23kzr8rq	163	24	formed	form	VBN
cord-324094-23kzr8rq	163	25	by	by	IN
cord-324094-23kzr8rq	163	26	annealing	anneal	VBG
cord-324094-23kzr8rq	163	27	between	between	IN
cord-324094-23kzr8rq	163	28	alternately	alternately	RB
cord-324094-23kzr8rq	163	29	inverted	invert	VBN
cord-324094-23kzr8rq	163	30	repeats	repeat	NNS
cord-324094-23kzr8rq	163	31	of	of	IN
cord-324094-23kzr8rq	163	32	the	the	DT
cord-324094-23kzr8rq	163	33	target	target	NN
cord-324094-23kzr8rq	163	34	in	in	IN
cord-324094-23kzr8rq	163	35	the	the	DT
cord-324094-23kzr8rq	163	36	same	same	JJ
cord-324094-23kzr8rq	163	37	strand	strand	NN
cord-324094-23kzr8rq	163	38	(	(	-LRB-
cord-324094-23kzr8rq	163	39	fi	fi	FW
cord-324094-23kzr8rq	163	40	gure	gure	NNP
cord-324094-23kzr8rq	163	41	4	4	CD
cord-324094-23kzr8rq	163	42	)	)	-RRB-
cord-324094-23kzr8rq	163	43	.	.	.
cord-324094-23kzr8rq	164	1	LAMP	LAMP	NNP
cord-324094-23kzr8rq	164	2	amplifi	amplifi	NNP
cord-324094-23kzr8rq	164	3	cation	cation	NN
cord-324094-23kzr8rq	164	4	can	can	MD
cord-324094-23kzr8rq	164	5	also	also	RB
cord-324094-23kzr8rq	164	6	be	be	VB
cord-324094-23kzr8rq	164	7	accomplished	accomplish	VBN
cord-324094-23kzr8rq	164	8	with	with	IN
cord-324094-23kzr8rq	164	9	the	the	DT
cord-324094-23kzr8rq	164	10	two	two	CD
cord-324094-23kzr8rq	164	11	outer	outer	JJ
cord-324094-23kzr8rq	164	12	(	(	-LRB-
cord-324094-23kzr8rq	164	13	F3	F3	NNP
cord-324094-23kzr8rq	164	14	and	and	CC
cord-324094-23kzr8rq	164	15	B3	B3	NNP
cord-324094-23kzr8rq	164	16	)	)	-RRB-
cord-324094-23kzr8rq	164	17	and	and	CC
cord-324094-23kzr8rq	164	18	two	two	CD
cord-324094-23kzr8rq	164	19	internal	internal	JJ
cord-324094-23kzr8rq	164	20	primers	primer	NNS
cord-324094-23kzr8rq	164	21	(	(	-LRB-
cord-324094-23kzr8rq	164	22	FIP	FIP	NNP
cord-324094-23kzr8rq	164	23	and	and	CC
cord-324094-23kzr8rq	164	24	BIP	BIP	NNP
cord-324094-23kzr8rq	164	25	)	)	-RRB-
cord-324094-23kzr8rq	164	26	but	but	CC
cord-324094-23kzr8rq	164	27	by	by	IN
cord-324094-23kzr8rq	164	28	using	use	VBG
cord-324094-23kzr8rq	164	29	the	the	DT
cord-324094-23kzr8rq	164	30	two	two	CD
cord-324094-23kzr8rq	164	31	loop	loop	NN
cord-324094-23kzr8rq	164	32	primers	primer	NNS
cord-324094-23kzr8rq	164	33	(	(	-LRB-
cord-324094-23kzr8rq	164	34	FLP	FLP	NNP
cord-324094-23kzr8rq	164	35	and	and	CC
cord-324094-23kzr8rq	164	36	BLP	BLP	NNP
cord-324094-23kzr8rq	164	37	)	)	-RRB-
cord-324094-23kzr8rq	164	38	,	,	,
cord-324094-23kzr8rq	164	39	the	the	DT
cord-324094-23kzr8rq	164	40	amplifi	amplifi	NNP
cord-324094-23kzr8rq	164	41	cation	cation	NN
cord-324094-23kzr8rq	164	42	is	be	VBZ
cord-324094-23kzr8rq	164	43	accelerated	accelerate	VBN
cord-324094-23kzr8rq	164	44	and	and	CC
cord-324094-23kzr8rq	164	45	thereby	thereby	RB
cord-324094-23kzr8rq	164	46	reducing	reduce	VBG
cord-324094-23kzr8rq	164	47	the	the	DT
cord-324094-23kzr8rq	164	48	amplifi	amplifi	NNP
cord-324094-23kzr8rq	164	49	cation	cation	NN
cord-324094-23kzr8rq	164	50	time	time	NN
cord-324094-23kzr8rq	164	51	to	to	IN
cord-324094-23kzr8rq	164	52	almost	almost	RB
cord-324094-23kzr8rq	164	53	half	half	DT
cord-324094-23kzr8rq	164	54	(	(	-LRB-
cord-324094-23kzr8rq	164	55	Nagamine	Nagamine	NNP
cord-324094-23kzr8rq	164	56	et	et	NNP
cord-324094-23kzr8rq	164	57	al	al	NNP
cord-324094-23kzr8rq	164	58	2002	2002	CD
cord-324094-23kzr8rq	164	59	)	)	-RRB-
cord-324094-23kzr8rq	164	60	.	.	.
cord-324094-23kzr8rq	165	1	The	the	DT
cord-324094-23kzr8rq	165	2	LAMP	LAMP	NNP
cord-324094-23kzr8rq	165	3	reaction	reaction	NN
cord-324094-23kzr8rq	165	4	is	be	VBZ
cord-324094-23kzr8rq	165	5	usually	usually	RB
cord-324094-23kzr8rq	165	6	carried	carry	VBN
cord-324094-23kzr8rq	165	7	out	out	RP
cord-324094-23kzr8rq	165	8	in	in	IN
cord-324094-23kzr8rq	165	9	a	a	DT
cord-324094-23kzr8rq	165	10	total	total	JJ
cord-324094-23kzr8rq	165	11	25	25	CD
cord-324094-23kzr8rq	165	12	μl	μl	IN
cord-324094-23kzr8rq	165	13	reaction	reaction	NN
cord-324094-23kzr8rq	165	14	volume	volume	NN
cord-324094-23kzr8rq	165	15	containing	contain	VBG
cord-324094-23kzr8rq	165	16	50	50	CD
cord-324094-23kzr8rq	165	17	pmol	pmol	NN
cord-324094-23kzr8rq	165	18	each	each	DT
cord-324094-23kzr8rq	165	19	of	of	IN
cord-324094-23kzr8rq	165	20	the	the	DT
cord-324094-23kzr8rq	165	21	primers	primer	NNS
cord-324094-23kzr8rq	165	22	FIP	FIP	NNP
cord-324094-23kzr8rq	165	23	and	and	CC
cord-324094-23kzr8rq	165	24	BIP	BIP	NNP
cord-324094-23kzr8rq	165	25	,	,	,
cord-324094-23kzr8rq	165	26	5	5	CD
cord-324094-23kzr8rq	165	27	pmol	pmol	NN
cord-324094-23kzr8rq	165	28	each	each	DT
cord-324094-23kzr8rq	165	29	of	of	IN
cord-324094-23kzr8rq	165	30	the	the	DT
cord-324094-23kzr8rq	165	31	outer	outer	JJ
cord-324094-23kzr8rq	165	32	primers	primer	NNS
cord-324094-23kzr8rq	165	33	F3	F3	NNP
cord-324094-23kzr8rq	165	34	and	and	CC
cord-324094-23kzr8rq	165	35	B3	b3	NN
cord-324094-23kzr8rq	165	36	,	,	,
cord-324094-23kzr8rq	165	37	25	25	CD
cord-324094-23kzr8rq	165	38	pmol	pmol	NN
cord-324094-23kzr8rq	165	39	each	each	DT
cord-324094-23kzr8rq	165	40	of	of	IN
cord-324094-23kzr8rq	165	41	loop	loop	NN
cord-324094-23kzr8rq	165	42	primers	primer	NNS
cord-324094-23kzr8rq	165	43	FLP	FLP	NNP
cord-324094-23kzr8rq	165	44	and	and	CC
cord-324094-23kzr8rq	165	45	BLP	BLP	NNP
cord-324094-23kzr8rq	165	46	in	in	IN
cord-324094-23kzr8rq	165	47	a	a	DT
cord-324094-23kzr8rq	165	48	2x	2x	CD
cord-324094-23kzr8rq	165	49	reaction	reaction	NN
cord-324094-23kzr8rq	165	50	mixture	mixture	NN
cord-324094-23kzr8rq	165	51	having	have	VBG
cord-324094-23kzr8rq	165	52	20	20	CD
cord-324094-23kzr8rq	165	53	mM	mM	NNP
cord-324094-23kzr8rq	165	54	Tris	Tris	NNP
cord-324094-23kzr8rq	165	55	-	-	HYPH
cord-324094-23kzr8rq	165	56	HCl	HCl	NNP
cord-324094-23kzr8rq	165	57	pH	pH	NNP
cord-324094-23kzr8rq	165	58	8.8	8.8	CD
cord-324094-23kzr8rq	165	59	,	,	,
cord-324094-23kzr8rq	165	60	10	10	CD
cord-324094-23kzr8rq	165	61	mM	mm	CD
cord-324094-23kzr8rq	165	62	(	(	-LRB-
cord-324094-23kzr8rq	165	63	NH	NH	NNP
cord-324094-23kzr8rq	165	64	4	4	CD
cord-324094-23kzr8rq	165	65	)	)	-RRB-
cord-324094-23kzr8rq	165	66	2	2	CD
cord-324094-23kzr8rq	165	67	SO	so	IN
cord-324094-23kzr8rq	165	68	4	4	CD
cord-324094-23kzr8rq	165	69	,	,	,
cord-324094-23kzr8rq	166	1	8	8	CD
cord-324094-23kzr8rq	166	2	mM	mm	CD
cord-324094-23kzr8rq	166	3	MgSO	MgSO	NNP
cord-324094-23kzr8rq	166	4	4	4	CD
cord-324094-23kzr8rq	166	5	,	,	,
cord-324094-23kzr8rq	167	1	10	10	CD
cord-324094-23kzr8rq	167	2	mM	mm	CD
cord-324094-23kzr8rq	167	3	KCl	KCl	NNS
cord-324094-23kzr8rq	167	4	,	,	,
cord-324094-23kzr8rq	167	5	1.4	1.4	CD
cord-324094-23kzr8rq	167	6	mM	mm	CD
cord-324094-23kzr8rq	167	7	dNTPs	dNTPs	NNP
cord-324094-23kzr8rq	167	8	,	,	,
cord-324094-23kzr8rq	167	9	0.8	0.8	CD
cord-324094-23kzr8rq	167	10	M	M	NNP
cord-324094-23kzr8rq	167	11	Betaine	Betaine	NNP
cord-324094-23kzr8rq	167	12	,	,	,
cord-324094-23kzr8rq	167	13	0.1	0.1	CD
cord-324094-23kzr8rq	167	14	%	%	NN
cord-324094-23kzr8rq	167	15	Tween20	Tween20	NNP
cord-324094-23kzr8rq	167	16	,	,	,
cord-324094-23kzr8rq	167	17	8	8	CD
cord-324094-23kzr8rq	167	18	units	unit	NNS
cord-324094-23kzr8rq	167	19	of	of	IN
cord-324094-23kzr8rq	167	20	the	the	DT
cord-324094-23kzr8rq	167	21	Bst	Bst	NNP
cord-324094-23kzr8rq	167	22	DNA	dna	NN
cord-324094-23kzr8rq	167	23	polymerase	polymerase	NN
cord-324094-23kzr8rq	167	24	(	(	-LRB-
cord-324094-23kzr8rq	167	25	New	New	NNP
cord-324094-23kzr8rq	167	26	England	England	NNP
cord-324094-23kzr8rq	167	27	Biolabs	Biolabs	NNP
cord-324094-23kzr8rq	167	28	)	)	-RRB-
cord-324094-23kzr8rq	167	29	,	,	,
cord-324094-23kzr8rq	167	30	and	and	CC
cord-324094-23kzr8rq	167	31	2	2	CD
cord-324094-23kzr8rq	167	32	μl	μl	NN
cord-324094-23kzr8rq	167	33	of	of	IN
cord-324094-23kzr8rq	167	34	DNA	dna	NN
cord-324094-23kzr8rq	167	35	template	template	NN
cord-324094-23kzr8rq	167	36	.	.	.
cord-324094-23kzr8rq	168	1	Positive	positive	JJ
cord-324094-23kzr8rq	168	2	and	and	CC
cord-324094-23kzr8rq	168	3	negative	negative	JJ
cord-324094-23kzr8rq	168	4	controls	control	NNS
cord-324094-23kzr8rq	168	5	should	should	MD
cord-324094-23kzr8rq	168	6	be	be	VB
cord-324094-23kzr8rq	168	7	included	include	VBN
cord-324094-23kzr8rq	168	8	in	in	IN
cord-324094-23kzr8rq	168	9	each	each	DT
cord-324094-23kzr8rq	168	10	run	run	NN
cord-324094-23kzr8rq	168	11	,	,	,
cord-324094-23kzr8rq	168	12	and	and	CC
cord-324094-23kzr8rq	168	13	all	all	DT
cord-324094-23kzr8rq	168	14	precautions	precaution	NNS
cord-324094-23kzr8rq	168	15	to	to	TO
cord-324094-23kzr8rq	168	16	prevent	prevent	VB
cord-324094-23kzr8rq	168	17	cross	cross	NN
cord-324094-23kzr8rq	168	18	-	-	NN
cord-324094-23kzr8rq	168	19	contamination	contamination	NN
cord-324094-23kzr8rq	168	20	should	should	MD
cord-324094-23kzr8rq	168	21	be	be	VB
cord-324094-23kzr8rq	168	22	observed	observe	VBN
cord-324094-23kzr8rq	168	23	.	.	.
cord-324094-23kzr8rq	169	1	The	the	DT
cord-324094-23kzr8rq	169	2	studies	study	NNS
cord-324094-23kzr8rq	169	3	temperature	temperature	NN
cord-324094-23kzr8rq	169	4	optima	optima	NNP
cord-324094-23kzr8rq	169	5	required	require	VBN
cord-324094-23kzr8rq	169	6	for	for	IN
cord-324094-23kzr8rq	169	7	effi	effi	NNP
cord-324094-23kzr8rq	169	8	cient	cient	NNP
cord-324094-23kzr8rq	169	9	amplifi	amplifi	NNP
cord-324094-23kzr8rq	169	10	cation	cation	NN
cord-324094-23kzr8rq	169	11	by	by	IN
cord-324094-23kzr8rq	169	12	LAMP	LAMP	NNP
cord-324094-23kzr8rq	169	13	assay	assay	NN
cord-324094-23kzr8rq	169	14	indicated	indicate	VBD
cord-324094-23kzr8rq	169	15	that	that	IN
cord-324094-23kzr8rq	169	16	the	the	DT
cord-324094-23kzr8rq	169	17	optimum	optimum	JJ
cord-324094-23kzr8rq	169	18	temperature	temperature	NN
cord-324094-23kzr8rq	169	19	for	for	IN
cord-324094-23kzr8rq	169	20	the	the	DT
cord-324094-23kzr8rq	169	21	LAMP	LAMP	NNP
cord-324094-23kzr8rq	169	22	reaction	reaction	NN
cord-324094-23kzr8rq	169	23	was	be	VBD
cord-324094-23kzr8rq	169	24	63	63	CD
cord-324094-23kzr8rq	169	25	°	°	NN
cord-324094-23kzr8rq	169	26	C	c	NN
cord-324094-23kzr8rq	169	27	,	,	,
cord-324094-23kzr8rq	169	28	which	which	WDT
cord-324094-23kzr8rq	169	29	is	be	VBZ
cord-324094-23kzr8rq	169	30	optimum	optimum	JJ
cord-324094-23kzr8rq	169	31	for	for	IN
cord-324094-23kzr8rq	169	32	the	the	DT
cord-324094-23kzr8rq	169	33	activity	activity	NN
cord-324094-23kzr8rq	169	34	of	of	IN
cord-324094-23kzr8rq	169	35	Bst	Bst	NNP
cord-324094-23kzr8rq	169	36	DNA	dna	NN
cord-324094-23kzr8rq	169	37	polymerase	polymerase	NN
cord-324094-23kzr8rq	169	38	.	.	.
cord-324094-23kzr8rq	170	1	The	the	DT
cord-324094-23kzr8rq	170	2	amplifi	amplifi	NNP
cord-324094-23kzr8rq	170	3	cation	cation	NN
cord-324094-23kzr8rq	170	4	of	of	IN
cord-324094-23kzr8rq	170	5	RNA	RNA	NNP
cord-324094-23kzr8rq	170	6	template	template	NN
cord-324094-23kzr8rq	170	7	was	be	VBD
cord-324094-23kzr8rq	170	8	accomplished	accomplish	VBN
cord-324094-23kzr8rq	170	9	through	through	IN
cord-324094-23kzr8rq	170	10	RT	RT	NNP
cord-324094-23kzr8rq	170	11	-	-	HYPH
cord-324094-23kzr8rq	170	12	LAMP	LAMP	NNP
cord-324094-23kzr8rq	170	13	assay	assay	NN
cord-324094-23kzr8rq	170	14	by	by	IN
cord-324094-23kzr8rq	170	15	employing	employ	VBG
cord-324094-23kzr8rq	170	16	reverse	reverse	JJ
cord-324094-23kzr8rq	170	17	transcriptase	transcriptase	NN
cord-324094-23kzr8rq	170	18	for	for	IN
cord-324094-23kzr8rq	170	19	reverse	reverse	JJ
cord-324094-23kzr8rq	170	20	transcription	transcription	NN
cord-324094-23kzr8rq	170	21	step	step	NN
cord-324094-23kzr8rq	170	22	in	in	IN
cord-324094-23kzr8rq	170	23	addition	addition	NN
cord-324094-23kzr8rq	170	24	to	to	IN
cord-324094-23kzr8rq	170	25	the	the	DT
cord-324094-23kzr8rq	170	26	Bst	Bst	NNP
cord-324094-23kzr8rq	170	27	DNA	dna	NN
cord-324094-23kzr8rq	170	28	polymerase	polymerase	NN
cord-324094-23kzr8rq	170	29	.	.	.
cord-324094-23kzr8rq	171	1	RT	RT	NNP
cord-324094-23kzr8rq	171	2	-	-	HYPH
cord-324094-23kzr8rq	171	3	LAMP	LAMP	NNP
cord-324094-23kzr8rq	171	4	method	method	NN
cord-324094-23kzr8rq	171	5	can	can	MD
cord-324094-23kzr8rq	171	6	synthesize	synthesize	VB
cord-324094-23kzr8rq	171	7	cDNA	cdna	NN
cord-324094-23kzr8rq	171	8	from	from	IN
cord-324094-23kzr8rq	171	9	template	template	NN
cord-324094-23kzr8rq	171	10	RNA	RNA	NNP
cord-324094-23kzr8rq	171	11	and	and	CC
cord-324094-23kzr8rq	171	12	apply	apply	VB
cord-324094-23kzr8rq	171	13	LAMP	LAMP	NNP
cord-324094-23kzr8rq	171	14	technology	technology	NN
cord-324094-23kzr8rq	171	15	to	to	TO
cord-324094-23kzr8rq	171	16	amplify	amplify	VB
cord-324094-23kzr8rq	171	17	and	and	CC
cord-324094-23kzr8rq	171	18	detect	detect	VB
cord-324094-23kzr8rq	171	19	them	-PRON-	PRP
cord-324094-23kzr8rq	171	20	.	.	.
cord-324094-23kzr8rq	172	1	The	the	DT
cord-324094-23kzr8rq	172	2	real	real	JJ
cord-324094-23kzr8rq	172	3	-	-	HYPH
cord-324094-23kzr8rq	172	4	time	time	NN
cord-324094-23kzr8rq	172	5	monitoring	monitoring	NN
cord-324094-23kzr8rq	172	6	of	of	IN
cord-324094-23kzr8rq	172	7	-	-	HYPH
cord-324094-23kzr8rq	172	8	LAMP	lamp	NN
cord-324094-23kzr8rq	172	9	amplifi	amplifi	NNP
cord-324094-23kzr8rq	172	10	cation	cation	NN
cord-324094-23kzr8rq	172	11	can	can	MD
cord-324094-23kzr8rq	172	12	be	be	VB
cord-324094-23kzr8rq	172	13	accomplished	accomplish	VBN
cord-324094-23kzr8rq	172	14	through	through	IN
cord-324094-23kzr8rq	172	15	spectrophotometric	spectrophotometric	JJ
cord-324094-23kzr8rq	172	16	analysis	analysis	NN
cord-324094-23kzr8rq	172	17	with	with	IN
cord-324094-23kzr8rq	172	18	the	the	DT
cord-324094-23kzr8rq	172	19	help	help	NN
cord-324094-23kzr8rq	172	20	of	of	IN
cord-324094-23kzr8rq	172	21	loop	loop	NN
cord-324094-23kzr8rq	172	22	amp	amp	NN
cord-324094-23kzr8rq	172	23	real	real	JJ
cord-324094-23kzr8rq	172	24	-	-	HYPH
cord-324094-23kzr8rq	172	25	time	time	NN
cord-324094-23kzr8rq	172	26	turbidimeter	turbidimeter	NNP
cord-324094-23kzr8rq	172	27	(	(	-LRB-
cord-324094-23kzr8rq	172	28	LA-200	LA-200	NNP
cord-324094-23kzr8rq	172	29	,	,	,
cord-324094-23kzr8rq	172	30	Teramecs	Teramecs	NNP
cord-324094-23kzr8rq	172	31	,	,	,
cord-324094-23kzr8rq	172	32	Japan	Japan	NNP
cord-324094-23kzr8rq	172	33	)	)	-RRB-
cord-324094-23kzr8rq	172	34	that	that	WDT
cord-324094-23kzr8rq	172	35	records	record	VBZ
cord-324094-23kzr8rq	172	36	the	the	DT
cord-324094-23kzr8rq	172	37	turbidity	turbidity	NN
cord-324094-23kzr8rq	172	38	in	in	IN
cord-324094-23kzr8rq	172	39	the	the	DT
cord-324094-23kzr8rq	172	40	form	form	NN
cord-324094-23kzr8rq	172	41	of	of	IN
cord-324094-23kzr8rq	172	42	OD	OD	NNP
cord-324094-23kzr8rq	172	43	at	at	IN
cord-324094-23kzr8rq	172	44	400	400	CD
cord-324094-23kzr8rq	172	45	nm	nm	NN
cord-324094-23kzr8rq	172	46	at	at	IN
cord-324094-23kzr8rq	172	47	every	every	DT
cord-324094-23kzr8rq	172	48	6	6	CD
cord-324094-23kzr8rq	172	49	second	second	NN
cord-324094-23kzr8rq	172	50	(	(	-LRB-
cord-324094-23kzr8rq	172	51	Mori	Mori	NNP
cord-324094-23kzr8rq	172	52	et	et	FW
cord-324094-23kzr8rq	172	53	al	al	NNP
cord-324094-23kzr8rq	172	54	2004	2004	CD
cord-324094-23kzr8rq	172	55	)	)	-RRB-
cord-324094-23kzr8rq	173	1	(	(	-LRB-
cord-324094-23kzr8rq	173	2	fi	fi	NNP
cord-324094-23kzr8rq	173	3	gures	gures	NNP
cord-324094-23kzr8rq	173	4	5	5	CD
cord-324094-23kzr8rq	173	5	,	,	,
cord-324094-23kzr8rq	173	6	6A	6a	CD
cord-324094-23kzr8rq	173	7	)	)	-RRB-
cord-324094-23kzr8rq	173	8	.	.	.
cord-324094-23kzr8rq	174	1	On	on	IN
cord-324094-23kzr8rq	174	2	agarose	agarose	JJ
cord-324094-23kzr8rq	174	3	gel	gel	NN
cord-324094-23kzr8rq	174	4	analysis	analysis	NN
cord-324094-23kzr8rq	174	5	,	,	,
cord-324094-23kzr8rq	174	6	the	the	DT
cord-324094-23kzr8rq	174	7	LAMP	LAMP	NNP
cord-324094-23kzr8rq	174	8	amplicons	amplicon	VBZ
cord-324094-23kzr8rq	174	9	revealed	reveal	VBD
cord-324094-23kzr8rq	174	10	ladder	ladder	NN
cord-324094-23kzr8rq	174	11	like	like	IN
cord-324094-23kzr8rq	174	12	pattern	pattern	NN
cord-324094-23kzr8rq	174	13	in	in	IN
cord-324094-23kzr8rq	174	14	contrast	contrast	NN
cord-324094-23kzr8rq	174	15	to	to	IN
cord-324094-23kzr8rq	174	16	a	a	DT
cord-324094-23kzr8rq	174	17	single	single	JJ
cord-324094-23kzr8rq	174	18	band	band	NN
cord-324094-23kzr8rq	174	19	as	as	IN
cord-324094-23kzr8rq	174	20	observed	observe	VBN
cord-324094-23kzr8rq	174	21	in	in	IN
cord-324094-23kzr8rq	174	22	PCR	PCR	NNP
cord-324094-23kzr8rq	174	23	(	(	-LRB-
cord-324094-23kzr8rq	174	24	fi	fi	FW
cord-324094-23kzr8rq	174	25	gure	gure	NNP
cord-324094-23kzr8rq	174	26	6B	6B	NNP
cord-324094-23kzr8rq	174	27	)	)	-RRB-
cord-324094-23kzr8rq	174	28	.	.	.
cord-324094-23kzr8rq	175	1	This	this	DT
cord-324094-23kzr8rq	175	2	is	be	VBZ
cord-324094-23kzr8rq	175	3	due	due	JJ
cord-324094-23kzr8rq	175	4	to	to	IN
cord-324094-23kzr8rq	175	5	the	the	DT
cord-324094-23kzr8rq	175	6	caulifl	caulifl	NNP
cord-324094-23kzr8rq	175	7	ower	ower	NNP
cord-324094-23kzr8rq	175	8	-	-	HYPH
cord-324094-23kzr8rq	175	9	like	like	JJ
cord-324094-23kzr8rq	175	10	structures	structure	NNS
cord-324094-23kzr8rq	175	11	with	with	IN
cord-324094-23kzr8rq	175	12	multiple	multiple	JJ
cord-324094-23kzr8rq	175	13	loops	loop	NNS
cord-324094-23kzr8rq	175	14	formed	form	VBN
cord-324094-23kzr8rq	175	15	by	by	IN
cord-324094-23kzr8rq	175	16	annealing	anneal	VBG
cord-324094-23kzr8rq	175	17	between	between	IN
cord-324094-23kzr8rq	175	18	alternately	alternately	RB
cord-324094-23kzr8rq	175	19	inverted	invert	VBN
cord-324094-23kzr8rq	175	20	repeats	repeat	NNS
cord-324094-23kzr8rq	175	21	of	of	IN
cord-324094-23kzr8rq	175	22	the	the	DT
cord-324094-23kzr8rq	175	23	target	target	NN
cord-324094-23kzr8rq	175	24	in	in	IN
cord-324094-23kzr8rq	175	25	the	the	DT
cord-324094-23kzr8rq	175	26	same	same	JJ
cord-324094-23kzr8rq	175	27	strand	strand	NN
cord-324094-23kzr8rq	175	28	.	.	.
cord-324094-23kzr8rq	176	1	In	in	IN
cord-324094-23kzr8rq	176	2	order	order	NN
cord-324094-23kzr8rq	176	3	to	to	TO
cord-324094-23kzr8rq	176	4	facilitate	facilitate	VB
cord-324094-23kzr8rq	176	5	the	the	DT
cord-324094-23kzr8rq	176	6	fi	fi	NNP
cord-324094-23kzr8rq	176	7	eld	eld	NNP
cord-324094-23kzr8rq	176	8	application	application	NN
cord-324094-23kzr8rq	176	9	of	of	IN
cord-324094-23kzr8rq	176	10	LAMP	LAMP	NNP
cord-324094-23kzr8rq	176	11	assay	assay	NN
cord-324094-23kzr8rq	176	12	,	,	,
cord-324094-23kzr8rq	176	13	the	the	DT
cord-324094-23kzr8rq	176	14	monitoring	monitoring	NN
cord-324094-23kzr8rq	176	15	of	of	IN
cord-324094-23kzr8rq	176	16	amplifi	amplifi	NNP
cord-324094-23kzr8rq	176	17	cation	cation	NN
cord-324094-23kzr8rq	176	18	can	can	MD
cord-324094-23kzr8rq	176	19	also	also	RB
cord-324094-23kzr8rq	176	20	be	be	VB
cord-324094-23kzr8rq	176	21	accomplished	accomplish	VBN
cord-324094-23kzr8rq	176	22	with	with	IN
cord-324094-23kzr8rq	176	23	naked	naked	JJ
cord-324094-23kzr8rq	176	24	eye	eye	NN
cord-324094-23kzr8rq	176	25	inspection	inspection	NN
cord-324094-23kzr8rq	176	26	either	either	CC
cord-324094-23kzr8rq	176	27	in	in	IN
cord-324094-23kzr8rq	176	28	the	the	DT
cord-324094-23kzr8rq	176	29	form	form	NN
cord-324094-23kzr8rq	176	30	of	of	IN
cord-324094-23kzr8rq	176	31	visual	visual	JJ
cord-324094-23kzr8rq	176	32	turbidity	turbidity	NN
cord-324094-23kzr8rq	176	33	or	or	CC
cord-324094-23kzr8rq	176	34	visual	visual	JJ
cord-324094-23kzr8rq	176	35	fl	fl	NNP
cord-324094-23kzr8rq	176	36	uorescence	uorescence	NN
cord-324094-23kzr8rq	176	37	.	.	.
cord-324094-23kzr8rq	177	1	Following	follow	VBG
cord-324094-23kzr8rq	177	2	amplifi	amplifi	NNP
cord-324094-23kzr8rq	177	3	cation	cation	NN
cord-324094-23kzr8rq	177	4	,	,	,
cord-324094-23kzr8rq	177	5	the	the	DT
cord-324094-23kzr8rq	177	6	tubes	tube	NNS
cord-324094-23kzr8rq	177	7	can	can	MD
cord-324094-23kzr8rq	177	8	be	be	VB
cord-324094-23kzr8rq	177	9	inspected	inspect	VBN
cord-324094-23kzr8rq	177	10	for	for	IN
cord-324094-23kzr8rq	177	11	white	white	JJ
cord-324094-23kzr8rq	177	12	turbidity	turbidity	NN
cord-324094-23kzr8rq	177	13	through	through	IN
cord-324094-23kzr8rq	177	14	naked	naked	JJ
cord-324094-23kzr8rq	177	15	eye	eye	NN
cord-324094-23kzr8rq	177	16	after	after	IN
cord-324094-23kzr8rq	177	17	a	a	DT
cord-324094-23kzr8rq	177	18	pulse	pulse	NN
cord-324094-23kzr8rq	177	19	spin	spin	NN
cord-324094-23kzr8rq	177	20	to	to	TO
cord-324094-23kzr8rq	177	21	deposit	deposit	VB
cord-324094-23kzr8rq	177	22	the	the	DT
cord-324094-23kzr8rq	177	23	precipitate	precipitate	NN
cord-324094-23kzr8rq	177	24	in	in	IN
cord-324094-23kzr8rq	177	25	the	the	DT
cord-324094-23kzr8rq	177	26	bottom	bottom	NN
cord-324094-23kzr8rq	177	27	of	of	IN
cord-324094-23kzr8rq	177	28	the	the	DT
cord-324094-23kzr8rq	177	29	tube	tube	NN
cord-324094-23kzr8rq	177	30	.	.	.
cord-324094-23kzr8rq	178	1	Hence	hence	RB
cord-324094-23kzr8rq	178	2	,	,	,
cord-324094-23kzr8rq	178	3	the	the	DT
cord-324094-23kzr8rq	178	4	presence	presence	NN
cord-324094-23kzr8rq	178	5	of	of	IN
cord-324094-23kzr8rq	178	6	turbidity	turbidity	NN
cord-324094-23kzr8rq	178	7	can	can	MD
cord-324094-23kzr8rq	178	8	indicate	indicate	VB
cord-324094-23kzr8rq	178	9	the	the	DT
cord-324094-23kzr8rq	178	10	presence	presence	NN
cord-324094-23kzr8rq	178	11	of	of	IN
cord-324094-23kzr8rq	178	12	target	target	NN
cord-324094-23kzr8rq	178	13	gene	gene	NN
cord-324094-23kzr8rq	178	14	(	(	-LRB-
cord-324094-23kzr8rq	178	15	fi	fi	FW
cord-324094-23kzr8rq	178	16	gure	gure	NNP
cord-324094-23kzr8rq	178	17	6C	6C	NNP
cord-324094-23kzr8rq	178	18	)	)	-RRB-
cord-324094-23kzr8rq	178	19	.The	.The	.
cord-324094-23kzr8rq	178	20	tube	tube	NN
cord-324094-23kzr8rq	178	21	containing	contain	VBG
cord-324094-23kzr8rq	178	22	the	the	DT
cord-324094-23kzr8rq	178	23	amplifi	amplifi	NNP
cord-324094-23kzr8rq	178	24	ed	ed	NN
cord-324094-23kzr8rq	178	25	products	product	NNS
cord-324094-23kzr8rq	178	26	can	can	MD
cord-324094-23kzr8rq	178	27	also	also	RB
cord-324094-23kzr8rq	178	28	be	be	VB
cord-324094-23kzr8rq	178	29	better	well	RBR
cord-324094-23kzr8rq	178	30	visualized	visualize	VBN
cord-324094-23kzr8rq	178	31	in	in	IN
cord-324094-23kzr8rq	178	32	the	the	DT
cord-324094-23kzr8rq	178	33	presence	presence	NN
cord-324094-23kzr8rq	178	34	of	of	IN
cord-324094-23kzr8rq	178	35	fl	fl	NNP
cord-324094-23kzr8rq	178	36	uorescent	uorescent	NNP
cord-324094-23kzr8rq	178	37	intercalating	intercalating	NN
cord-324094-23kzr8rq	178	38	dye	dye	NN
cord-324094-23kzr8rq	178	39	viz	viz	NNP
cord-324094-23kzr8rq	178	40	.	.	.
cord-324094-23kzr8rq	179	1	ethidium	ethidium	NN
cord-324094-23kzr8rq	179	2	bromide	bromide	NN
cord-324094-23kzr8rq	179	3	,	,	,
cord-324094-23kzr8rq	179	4	SYBR	SYBR	NNP
cord-324094-23kzr8rq	179	5	Green	Green	NNP
cord-324094-23kzr8rq	179	6	I	I	NNP
cord-324094-23kzr8rq	179	7	and	and	CC
cord-324094-23kzr8rq	179	8	Calcein	Calcein	NNP
cord-324094-23kzr8rq	179	9	etc	etc	FW
cord-324094-23kzr8rq	179	10	.	.	.
cord-324094-23kzr8rq	180	1	by	by	IN
cord-324094-23kzr8rq	180	2	illuminating	illuminate	VBG
cord-324094-23kzr8rq	180	3	with	with	IN
cord-324094-23kzr8rq	180	4	a	a	DT
cord-324094-23kzr8rq	180	5	UV	uv	NN
cord-324094-23kzr8rq	180	6	lamp	lamp	NN
cord-324094-23kzr8rq	180	7	,	,	,
cord-324094-23kzr8rq	180	8	the	the	DT
cord-324094-23kzr8rq	180	9	fl	fl	NNP
cord-324094-23kzr8rq	180	10	uorescence	uorescence	NN
cord-324094-23kzr8rq	180	11	intensity	intensity	NN
cord-324094-23kzr8rq	180	12	increases	increase	NNS
cord-324094-23kzr8rq	180	13	.	.	.
cord-324094-23kzr8rq	181	1	In	in	IN
cord-324094-23kzr8rq	181	2	practice	practice	NN
cord-324094-23kzr8rq	181	3	,	,	,
cord-324094-23kzr8rq	181	4	usually	usually	RB
cord-324094-23kzr8rq	181	5	the	the	DT
cord-324094-23kzr8rq	181	6	visual	visual	JJ
cord-324094-23kzr8rq	181	7	inspection	inspection	NN
cord-324094-23kzr8rq	181	8	for	for	IN
cord-324094-23kzr8rq	181	9	amplifi	amplifi	NNP
cord-324094-23kzr8rq	181	10	cation	cation	NN
cord-324094-23kzr8rq	181	11	is	be	VBZ
cord-324094-23kzr8rq	181	12	performed	perform	VBN
cord-324094-23kzr8rq	181	13	through	through	IN
cord-324094-23kzr8rq	181	14	observation	observation	NN
cord-324094-23kzr8rq	181	15	of	of	IN
cord-324094-23kzr8rq	181	16	color	color	NN
cord-324094-23kzr8rq	181	17	change	change	NN
cord-324094-23kzr8rq	181	18	following	follow	VBG
cord-324094-23kzr8rq	181	19	addition	addition	NN
cord-324094-23kzr8rq	181	20	of	of	IN
cord-324094-23kzr8rq	181	21	1	1	CD
cord-324094-23kzr8rq	181	22	μl	μl	NN
cord-324094-23kzr8rq	181	23	of	of	IN
cord-324094-23kzr8rq	181	24	SYBR	SYBR	NNP
cord-324094-23kzr8rq	181	25	Green	Green	NNP
cord-324094-23kzr8rq	182	1	I	-PRON-	PRP
cord-324094-23kzr8rq	182	2	(	(	-LRB-
cord-324094-23kzr8rq	182	3	a	a	DT
cord-324094-23kzr8rq	182	4	fl	fl	NNP
cord-324094-23kzr8rq	182	5	uorescent	uorescent	NNP
cord-324094-23kzr8rq	182	6	dsDNA	dsDNA	NNP
cord-324094-23kzr8rq	182	7	intercalating	intercalate	VBG
cord-324094-23kzr8rq	182	8	dye	dye	NN
cord-324094-23kzr8rq	182	9	)	)	-RRB-
cord-324094-23kzr8rq	182	10	to	to	IN
cord-324094-23kzr8rq	182	11	the	the	DT
cord-324094-23kzr8rq	182	12	tube	tube	NN
cord-324094-23kzr8rq	182	13	.	.	.
cord-324094-23kzr8rq	183	1	In	in	IN
cord-324094-23kzr8rq	183	2	case	case	NN
cord-324094-23kzr8rq	183	3	of	of	IN
cord-324094-23kzr8rq	183	4	positive	positive	JJ
cord-324094-23kzr8rq	183	5	amplifi	amplifi	NNP
cord-324094-23kzr8rq	183	6	cation	cation	NN
cord-324094-23kzr8rq	183	7	,	,	,
cord-324094-23kzr8rq	183	8	the	the	DT
cord-324094-23kzr8rq	183	9	original	original	JJ
cord-324094-23kzr8rq	183	10	orange	orange	JJ
cord-324094-23kzr8rq	183	11	color	color	NN
cord-324094-23kzr8rq	183	12	of	of	IN
cord-324094-23kzr8rq	183	13	the	the	DT
cord-324094-23kzr8rq	183	14	dye	dye	NN
cord-324094-23kzr8rq	183	15	will	will	MD
cord-324094-23kzr8rq	183	16	change	change	VB
cord-324094-23kzr8rq	183	17	into	into	IN
cord-324094-23kzr8rq	183	18	green	green	JJ
cord-324094-23kzr8rq	183	19	that	that	WDT
cord-324094-23kzr8rq	183	20	can	can	MD
cord-324094-23kzr8rq	183	21	be	be	VB
cord-324094-23kzr8rq	183	22	judged	judge	VBN
cord-324094-23kzr8rq	183	23	under	under	IN
cord-324094-23kzr8rq	183	24	natural	natural	JJ
cord-324094-23kzr8rq	183	25	light	light	NN
cord-324094-23kzr8rq	183	26	as	as	RB
cord-324094-23kzr8rq	183	27	well	well	RB
cord-324094-23kzr8rq	183	28	as	as	IN
cord-324094-23kzr8rq	183	29	under	under	IN
cord-324094-23kzr8rq	183	30	UV	uv	NN
cord-324094-23kzr8rq	183	31	light	light	NN
cord-324094-23kzr8rq	183	32	(	(	-LRB-
cord-324094-23kzr8rq	183	33	302	302	CD
cord-324094-23kzr8rq	183	34	nm	nm	CD
cord-324094-23kzr8rq	183	35	)	)	-RRB-
cord-324094-23kzr8rq	183	36	with	with	IN
cord-324094-23kzr8rq	183	37	the	the	DT
cord-324094-23kzr8rq	183	38	help	help	NN
cord-324094-23kzr8rq	183	39	of	of	IN
cord-324094-23kzr8rq	183	40	a	a	DT
cord-324094-23kzr8rq	183	41	hand	hand	NN
cord-324094-23kzr8rq	183	42	held	hold	VBN
cord-324094-23kzr8rq	183	43	UV	uv	NN
cord-324094-23kzr8rq	183	44	torch	torch	NN
cord-324094-23kzr8rq	183	45	lamp	lamp	NN
cord-324094-23kzr8rq	183	46	.	.	.
cord-324094-23kzr8rq	184	1	In	in	IN
cord-324094-23kzr8rq	184	2	case	case	NN
cord-324094-23kzr8rq	184	3	there	there	EX
cord-324094-23kzr8rq	184	4	is	be	VBZ
cord-324094-23kzr8rq	184	5	no	no	DT
cord-324094-23kzr8rq	184	6	amplifi	amplifi	NNP
cord-324094-23kzr8rq	184	7	cation	cation	NN
cord-324094-23kzr8rq	184	8	,	,	,
cord-324094-23kzr8rq	184	9	the	the	DT
cord-324094-23kzr8rq	184	10	original	original	JJ
cord-324094-23kzr8rq	184	11	orange	orange	JJ
cord-324094-23kzr8rq	184	12	color	color	NN
cord-324094-23kzr8rq	184	13	of	of	IN
cord-324094-23kzr8rq	184	14	the	the	DT
cord-324094-23kzr8rq	184	15	dye	dye	NN
cord-324094-23kzr8rq	184	16	will	will	MD
cord-324094-23kzr8rq	184	17	be	be	VB
cord-324094-23kzr8rq	184	18	retained	retain	VBN
cord-324094-23kzr8rq	184	19	.	.	.
cord-324094-23kzr8rq	185	1	This	this	DT
cord-324094-23kzr8rq	185	2	change	change	NN
cord-324094-23kzr8rq	185	3	of	of	IN
cord-324094-23kzr8rq	185	4	color	color	NN
cord-324094-23kzr8rq	185	5	is	be	VBZ
cord-324094-23kzr8rq	185	6	permanent	permanent	JJ
cord-324094-23kzr8rq	185	7	and	and	CC
cord-324094-23kzr8rq	185	8	thus	thus	RB
cord-324094-23kzr8rq	185	9	can	can	MD
cord-324094-23kzr8rq	185	10	be	be	VB
cord-324094-23kzr8rq	185	11	kept	keep	VBN
cord-324094-23kzr8rq	185	12	for	for	IN
cord-324094-23kzr8rq	185	13	record	record	NN
cord-324094-23kzr8rq	185	14	purpose	purpose	NN
cord-324094-23kzr8rq	185	15	(	(	-LRB-
cord-324094-23kzr8rq	185	16	fi	fi	NNP
cord-324094-23kzr8rq	185	17	gure	gure	NNP
cord-324094-23kzr8rq	185	18	6D	6D	NNP
cord-324094-23kzr8rq	185	19	)	)	-RRB-
cord-324094-23kzr8rq	185	20	.	.	.
cord-324094-23kzr8rq	186	1	In	in	IN
cord-324094-23kzr8rq	186	2	order	order	NN
cord-324094-23kzr8rq	186	3	to	to	TO
cord-324094-23kzr8rq	186	4	detect	detect	VB
cord-324094-23kzr8rq	186	5	LAMP	LAMP	NNP
cord-324094-23kzr8rq	186	6	products	product	NNS
cord-324094-23kzr8rq	186	7	in	in	IN
cord-324094-23kzr8rq	186	8	a	a	DT
cord-324094-23kzr8rq	186	9	sequencespecifi	sequencespecifi	NNP
cord-324094-23kzr8rq	186	10	c	c	NN
cord-324094-23kzr8rq	186	11	manner	manner	NN
cord-324094-23kzr8rq	186	12	visually	visually	RB
cord-324094-23kzr8rq	186	13	,	,	,
cord-324094-23kzr8rq	186	14	an	an	DT
cord-324094-23kzr8rq	186	15	extremely	extremely	RB
cord-324094-23kzr8rq	186	16	simple	simple	JJ
cord-324094-23kzr8rq	186	17	method	method	NN
cord-324094-23kzr8rq	186	18	was	be	VBD
cord-324094-23kzr8rq	186	19	reported	report	VBN
cord-324094-23kzr8rq	186	20	by	by	IN
cord-324094-23kzr8rq	186	21	adding	add	VBG
cord-324094-23kzr8rq	186	22	a	a	DT
cord-324094-23kzr8rq	186	23	small	small	JJ
cord-324094-23kzr8rq	186	24	amount	amount	NN
cord-324094-23kzr8rq	186	25	of	of	IN
cord-324094-23kzr8rq	186	26	low	low	JJ
cord-324094-23kzr8rq	186	27	-	-	HYPH
cord-324094-23kzr8rq	186	28	molecular	molecular	JJ
cord-324094-23kzr8rq	186	29	weight	weight	NN
cord-324094-23kzr8rq	186	30	polyethylenimine	polyethylenimine	NN
cord-324094-23kzr8rq	186	31	(	(	-LRB-
cord-324094-23kzr8rq	186	32	PEI	PEI	NNP
cord-324094-23kzr8rq	186	33	)	)	-RRB-
cord-324094-23kzr8rq	186	34	to	to	IN
cord-324094-23kzr8rq	186	35	the	the	DT
cord-324094-23kzr8rq	186	36	LAMP	LAMP	NNP
cord-324094-23kzr8rq	186	37	reaction	reaction	NN
cord-324094-23kzr8rq	186	38	solution	solution	NN
cord-324094-23kzr8rq	186	39	.	.	.
cord-324094-23kzr8rq	187	1	The	the	DT
cord-324094-23kzr8rq	187	2	biggest	big	JJS
cord-324094-23kzr8rq	187	3	feature	feature	NN
cord-324094-23kzr8rq	187	4	of	of	IN
cord-324094-23kzr8rq	187	5	this	this	DT
cord-324094-23kzr8rq	187	6	technique	technique	NN
cord-324094-23kzr8rq	187	7	is	be	VBZ
cord-324094-23kzr8rq	187	8	the	the	DT
cord-324094-23kzr8rq	187	9	ability	ability	NN
cord-324094-23kzr8rq	187	10	to	to	TO
cord-324094-23kzr8rq	187	11	visually	visually	RB
cord-324094-23kzr8rq	187	12	present	present	JJ
cord-324094-23kzr8rq	187	13	sequence	sequence	NN
cord-324094-23kzr8rq	187	14	information	information	NN
cord-324094-23kzr8rq	187	15	of	of	IN
cord-324094-23kzr8rq	187	16	amplicons	amplicon	NNS
cord-324094-23kzr8rq	187	17	without	without	IN
cord-324094-23kzr8rq	187	18	using	use	VBG
cord-324094-23kzr8rq	187	19	an	an	DT
cord-324094-23kzr8rq	187	20	expensive	expensive	JJ
cord-324094-23kzr8rq	187	21	source	source	NN
cord-324094-23kzr8rq	187	22	of	of	IN
cord-324094-23kzr8rq	187	23	light	light	NN
cord-324094-23kzr8rq	187	24	or	or	CC
cord-324094-23kzr8rq	187	25	a	a	DT
cord-324094-23kzr8rq	187	26	detector	detector	NN
cord-324094-23kzr8rq	187	27	.	.	.
cord-324094-23kzr8rq	188	1	The	the	DT
cord-324094-23kzr8rq	188	2	new	new	JJ
cord-324094-23kzr8rq	188	3	detection	detection	NN
cord-324094-23kzr8rq	188	4	method	method	NN
cord-324094-23kzr8rq	188	5	described	describe	VBN
cord-324094-23kzr8rq	188	6	above	above	RB
cord-324094-23kzr8rq	188	7	utilizes	utilize	VBZ
cord-324094-23kzr8rq	188	8	the	the	DT
cord-324094-23kzr8rq	188	9	unique	unique	JJ
cord-324094-23kzr8rq	188	10	nature	nature	NN
cord-324094-23kzr8rq	188	11	of	of	IN
cord-324094-23kzr8rq	188	12	low	low	JJ
cord-324094-23kzr8rq	188	13	-	-	HYPH
cord-324094-23kzr8rq	188	14	molecular	molecular	JJ
cord-324094-23kzr8rq	188	15	-	-	HYPH
cord-324094-23kzr8rq	188	16	weight	weight	NN
cord-324094-23kzr8rq	188	17	PEI	PEI	NNP
cord-324094-23kzr8rq	188	18	,	,	,
cord-324094-23kzr8rq	188	19	i.e.	i.e.	FW
cord-324094-23kzr8rq	188	20	it	-PRON-	PRP
cord-324094-23kzr8rq	188	21	can	can	MD
cord-324094-23kzr8rq	188	22	not	not	RB
cord-324094-23kzr8rq	188	23	form	form	VB
cord-324094-23kzr8rq	188	24	an	an	DT
cord-324094-23kzr8rq	188	25	insoluble	insoluble	JJ
cord-324094-23kzr8rq	188	26	complex	complex	NN
cord-324094-23kzr8rq	188	27	with	with	IN
cord-324094-23kzr8rq	188	28	a	a	DT
cord-324094-23kzr8rq	188	29	single	single	RB
cord-324094-23kzr8rq	188	30	-	-	HYPH
cord-324094-23kzr8rq	188	31	stranded	strand	VBN
cord-324094-23kzr8rq	188	32	anionic	anionic	JJ
cord-324094-23kzr8rq	188	33	polymer	polymer	NN
cord-324094-23kzr8rq	188	34	with	with	IN
cord-324094-23kzr8rq	188	35	a	a	DT
cord-324094-23kzr8rq	188	36	low	low	JJ
cord-324094-23kzr8rq	188	37	molecular	molecular	JJ
cord-324094-23kzr8rq	188	38	weight	weight	NN
cord-324094-23kzr8rq	188	39	such	such	JJ
cord-324094-23kzr8rq	188	40	as	as	IN
cord-324094-23kzr8rq	188	41	an	an	DT
cord-324094-23kzr8rq	188	42	oligo	oligo	JJ
cord-324094-23kzr8rq	188	43	DNA	dna	NN
cord-324094-23kzr8rq	188	44	probe	probe	NN
cord-324094-23kzr8rq	188	45	,	,	,
cord-324094-23kzr8rq	188	46	but	but	CC
cord-324094-23kzr8rq	188	47	it	-PRON-	PRP
cord-324094-23kzr8rq	188	48	can	can	MD
cord-324094-23kzr8rq	188	49	form	form	VB
cord-324094-23kzr8rq	188	50	an	an	DT
cord-324094-23kzr8rq	188	51	insoluble	insoluble	JJ
cord-324094-23kzr8rq	188	52	complex	complex	NN
cord-324094-23kzr8rq	188	53	with	with	IN
cord-324094-23kzr8rq	188	54	DNA	dna	NN
cord-324094-23kzr8rq	188	55	with	with	IN
cord-324094-23kzr8rq	188	56	a	a	DT
cord-324094-23kzr8rq	188	57	high	high	JJ
cord-324094-23kzr8rq	188	58	molecular	molecular	JJ
cord-324094-23kzr8rq	188	59	weight	weight	NN
cord-324094-23kzr8rq	188	60	such	such	JJ
cord-324094-23kzr8rq	188	61	as	as	IN
cord-324094-23kzr8rq	188	62	LAMP	lamp	NN
cord-324094-23kzr8rq	188	63	product	product	NN
cord-324094-23kzr8rq	188	64	(	(	-LRB-
cord-324094-23kzr8rq	188	65	Mori	Mori	NNP
cord-324094-23kzr8rq	188	66	et	et	FW
cord-324094-23kzr8rq	188	67	al	al	NNP
cord-324094-23kzr8rq	188	68	2006	2006	CD
cord-324094-23kzr8rq	188	69	)	)	-RRB-
cord-324094-23kzr8rq	188	70	.	.	.
cord-324094-23kzr8rq	189	1	Capitalizing	capitalize	VBG
cord-324094-23kzr8rq	189	2	on	on	IN
cord-324094-23kzr8rq	189	3	its	-PRON-	PRP$
cord-324094-23kzr8rq	189	4	exquisite	exquisite	JJ
cord-324094-23kzr8rq	189	5	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	189	6	,	,	,
cord-324094-23kzr8rq	189	7	LAMP	LAMP	NNP
cord-324094-23kzr8rq	189	8	has	have	VBZ
cord-324094-23kzr8rq	189	9	been	be	VBN
cord-324094-23kzr8rq	189	10	designed	design	VBN
cord-324094-23kzr8rq	189	11	to	to	TO
cord-324094-23kzr8rq	189	12	quantify	quantify	VB
cord-324094-23kzr8rq	189	13	the	the	DT
cord-324094-23kzr8rq	189	14	amount	amount	NN
cord-324094-23kzr8rq	189	15	of	of	IN
cord-324094-23kzr8rq	189	16	gene	gene	NN
cord-324094-23kzr8rq	189	17	copies	copy	NNS
cord-324094-23kzr8rq	189	18	in	in	IN
cord-324094-23kzr8rq	189	19	a	a	DT
cord-324094-23kzr8rq	189	20	person	person	NN
cord-324094-23kzr8rq	189	21	's	's	POS
cord-324094-23kzr8rq	189	22	blood	blood	NN
cord-324094-23kzr8rq	189	23	(	(	-LRB-
cord-324094-23kzr8rq	189	24	load	load	NN
cord-324094-23kzr8rq	189	25	)	)	-RRB-
cord-324094-23kzr8rq	189	26	thereby	thereby	RB
cord-324094-23kzr8rq	189	27	allowing	allow	VBG
cord-324094-23kzr8rq	189	28	physicians	physician	NNS
cord-324094-23kzr8rq	189	29	to	to	TO
cord-324094-23kzr8rq	189	30	monitor	monitor	VB
cord-324094-23kzr8rq	189	31	their	-PRON-	PRP$
cord-324094-23kzr8rq	189	32	patients	patient	NNS
cord-324094-23kzr8rq	189	33	'	'	POS
cord-324094-23kzr8rq	189	34	disease	disease	NN
cord-324094-23kzr8rq	189	35	progression	progression	NN
cord-324094-23kzr8rq	189	36	and	and	CC
cord-324094-23kzr8rq	189	37	response	response	NN
cord-324094-23kzr8rq	189	38	to	to	IN
cord-324094-23kzr8rq	189	39	therapy	therapy	NN
cord-324094-23kzr8rq	189	40	.	.	.
cord-324094-23kzr8rq	190	1	Assessment	assessment	NN
cord-324094-23kzr8rq	190	2	of	of	IN
cord-324094-23kzr8rq	190	3	the	the	DT
cord-324094-23kzr8rq	190	4	load	load	NN
cord-324094-23kzr8rq	190	5	of	of	IN
cord-324094-23kzr8rq	190	6	the	the	DT
cord-324094-23kzr8rq	190	7	organisms	organism	NNS
cord-324094-23kzr8rq	190	8	/	/	SYM
cord-324094-23kzr8rq	190	9	pathogens	pathogen	NNS
cord-324094-23kzr8rq	190	10	before	before	RB
cord-324094-23kzr8rq	190	11	,	,	,
cord-324094-23kzr8rq	190	12	during	during	IN
cord-324094-23kzr8rq	190	13	and	and	CC
cord-324094-23kzr8rq	190	14	after	after	IN
cord-324094-23kzr8rq	190	15	therapy	therapy	NN
cord-324094-23kzr8rq	190	16	has	have	VBZ
cord-324094-23kzr8rq	190	17	tremendous	tremendous	JJ
cord-324094-23kzr8rq	190	18	potential	potential	NN
cord-324094-23kzr8rq	190	19	for	for	IN
cord-324094-23kzr8rq	190	20	improving	improve	VBG
cord-324094-23kzr8rq	190	21	the	the	DT
cord-324094-23kzr8rq	190	22	clinical	clinical	JJ
cord-324094-23kzr8rq	190	23	management	management	NN
cord-324094-23kzr8rq	190	24	of	of	IN
cord-324094-23kzr8rq	190	25	diseases	disease	NNS
cord-324094-23kzr8rq	190	26	.	.	.
cord-324094-23kzr8rq	191	1	The	the	DT
cord-324094-23kzr8rq	191	2	quantifi	quantifi	NN
cord-324094-23kzr8rq	191	3	cation	cation	NN
cord-324094-23kzr8rq	191	4	of	of	IN
cord-324094-23kzr8rq	191	5	gene	gene	NN
cord-324094-23kzr8rq	191	6	copy	copy	NN
cord-324094-23kzr8rq	191	7	number	number	NN
cord-324094-23kzr8rq	191	8	and/or	and/or	CC
cord-324094-23kzr8rq	191	9	concentration	concentration	NN
cord-324094-23kzr8rq	191	10	of	of	IN
cord-324094-23kzr8rq	191	11	the	the	DT
cord-324094-23kzr8rq	191	12	organisms	organism	NNS
cord-324094-23kzr8rq	191	13	can	can	MD
cord-324094-23kzr8rq	191	14	be	be	VB
cord-324094-23kzr8rq	191	15	accomplished	accomplish	VBN
cord-324094-23kzr8rq	191	16	through	through	IN
cord-324094-23kzr8rq	191	17	generation	generation	NN
cord-324094-23kzr8rq	191	18	of	of	IN
cord-324094-23kzr8rq	191	19	a	a	DT
cord-324094-23kzr8rq	191	20	standard	standard	JJ
cord-324094-23kzr8rq	191	21	curve	curve	NN
cord-324094-23kzr8rq	191	22	by	by	IN
cord-324094-23kzr8rq	191	23	plotting	plot	VBG
cord-324094-23kzr8rq	191	24	a	a	DT
cord-324094-23kzr8rq	191	25	graph	graph	NN
cord-324094-23kzr8rq	191	26	between	between	IN
cord-324094-23kzr8rq	191	27	known	know	VBN
cord-324094-23kzr8rq	191	28	concentration	concentration	NN
cord-324094-23kzr8rq	191	29	of	of	IN
cord-324094-23kzr8rq	191	30	gene	gene	NN
cord-324094-23kzr8rq	191	31	copy	copy	NN
cord-324094-23kzr8rq	191	32	number	number	NN
cord-324094-23kzr8rq	191	33	or	or	CC
cord-324094-23kzr8rq	191	34	infectious	infectious	JJ
cord-324094-23kzr8rq	191	35	unit	unit	NN
cord-324094-23kzr8rq	191	36	of	of	IN
cord-324094-23kzr8rq	191	37	organisms	organism	NNS
cord-324094-23kzr8rq	191	38	and	and	CC
cord-324094-23kzr8rq	191	39	time	time	NN
cord-324094-23kzr8rq	191	40	of	of	IN
cord-324094-23kzr8rq	191	41	positivity	positivity	NN
cord-324094-23kzr8rq	191	42	to	to	TO
cord-324094-23kzr8rq	191	43	get	get	VB
cord-324094-23kzr8rq	191	44	the	the	DT
cord-324094-23kzr8rq	191	45	amplifi	amplifi	NNP
cord-324094-23kzr8rq	191	46	cation	cation	NN
cord-324094-23kzr8rq	191	47	signal	signal	NN
cord-324094-23kzr8rq	191	48	for	for	IN
cord-324094-23kzr8rq	191	49	that	that	DT
cord-324094-23kzr8rq	191	50	particular	particular	JJ
cord-324094-23kzr8rq	191	51	concentration	concentration	NN
cord-324094-23kzr8rq	191	52	.	.	.
cord-324094-23kzr8rq	192	1	A	a	DT
cord-324094-23kzr8rq	192	2	linear	linear	JJ
cord-324094-23kzr8rq	192	3	relationship	relationship	NN
cord-324094-23kzr8rq	192	4	between	between	IN
cord-324094-23kzr8rq	192	5	various	various	JJ
cord-324094-23kzr8rq	192	6	concentrations	concentration	NNS
cord-324094-23kzr8rq	193	1	Vs	Vs	NNP
cord-324094-23kzr8rq	193	2	time	time	NN
cord-324094-23kzr8rq	193	3	of	of	IN
cord-324094-23kzr8rq	193	4	positivity	positivity	NN
cord-324094-23kzr8rq	193	5	is	be	VBZ
cord-324094-23kzr8rq	193	6	usually	usually	RB
cord-324094-23kzr8rq	193	7	obtained	obtain	VBN
cord-324094-23kzr8rq	193	8	through	through	IN
cord-324094-23kzr8rq	193	9	the	the	DT
cord-324094-23kzr8rq	193	10	real	real	JJ
cord-324094-23kzr8rq	193	11	-	-	HYPH
cord-324094-23kzr8rq	193	12	time	time	NN
cord-324094-23kzr8rq	193	13	monitoring	monitoring	NN
cord-324094-23kzr8rq	193	14	of	of	IN
cord-324094-23kzr8rq	193	15	the	the	DT
cord-324094-23kzr8rq	193	16	amplifi	amplifi	NNP
cord-324094-23kzr8rq	193	17	cation	cation	NN
cord-324094-23kzr8rq	193	18	(	(	-LRB-
cord-324094-23kzr8rq	193	19	fi	fi	NNP
cord-324094-23kzr8rq	193	20	gure	gure	NNP
cord-324094-23kzr8rq	193	21	7	7	CD
cord-324094-23kzr8rq	193	22	)	)	-RRB-
cord-324094-23kzr8rq	193	23	.	.	.
cord-324094-23kzr8rq	194	1	The	the	DT
cord-324094-23kzr8rq	194	2	quantifi	quantifi	NN
cord-324094-23kzr8rq	194	3	cation	cation	NN
cord-324094-23kzr8rq	194	4	of	of	IN
cord-324094-23kzr8rq	194	5	gene	gene	NN
cord-324094-23kzr8rq	194	6	copies	copy	NNS
cord-324094-23kzr8rq	194	7	in	in	IN
cord-324094-23kzr8rq	194	8	the	the	DT
cord-324094-23kzr8rq	194	9	clinical	clinical	JJ
cord-324094-23kzr8rq	194	10	samples	sample	NNS
cord-324094-23kzr8rq	194	11	can	can	MD
cord-324094-23kzr8rq	194	12	be	be	VB
cord-324094-23kzr8rq	194	13	extrapolated	extrapolate	VBN
cord-324094-23kzr8rq	194	14	from	from	IN
cord-324094-23kzr8rq	194	15	the	the	DT
cord-324094-23kzr8rq	194	16	standard	standard	JJ
cord-324094-23kzr8rq	194	17	curve	curve	NN
cord-324094-23kzr8rq	194	18	on	on	IN
cord-324094-23kzr8rq	194	19	the	the	DT
cord-324094-23kzr8rq	194	20	basis	basis	NN
cord-324094-23kzr8rq	194	21	of	of	IN
cord-324094-23kzr8rq	194	22	their	-PRON-	PRP$
cord-324094-23kzr8rq	194	23	time	time	NN
cord-324094-23kzr8rq	194	24	of	of	IN
cord-324094-23kzr8rq	194	25	positivity	positivity	NN
cord-324094-23kzr8rq	194	26	.	.	.
cord-324094-23kzr8rq	195	1	LAMP	lamp	NN
cord-324094-23kzr8rq	195	2	technology	technology	NN
cord-324094-23kzr8rq	195	3	facilitates	facilitate	VBZ
cord-324094-23kzr8rq	195	4	the	the	DT
cord-324094-23kzr8rq	195	5	detection	detection	NN
cord-324094-23kzr8rq	195	6	of	of	IN
cord-324094-23kzr8rq	195	7	DNA	DNA	NNP
cord-324094-23kzr8rq	195	8	or	or	CC
cord-324094-23kzr8rq	195	9	RNA	RNA	NNP
cord-324094-23kzr8rq	195	10	of	of	IN
cord-324094-23kzr8rq	195	11	pathogenic	pathogenic	JJ
cord-324094-23kzr8rq	195	12	organisms	organism	NNS
cord-324094-23kzr8rq	195	13	and	and	CC
cord-324094-23kzr8rq	195	14	,	,	,
cord-324094-23kzr8rq	195	15	as	as	IN
cord-324094-23kzr8rq	195	16	such	such	JJ
cord-324094-23kzr8rq	195	17	,	,	,
cord-324094-23kzr8rq	195	18	is	be	VBZ
cord-324094-23kzr8rq	195	19	the	the	DT
cord-324094-23kzr8rq	195	20	basis	basis	NN
cord-324094-23kzr8rq	195	21	for	for	IN
cord-324094-23kzr8rq	195	22	a	a	DT
cord-324094-23kzr8rq	195	23	broad	broad	JJ
cord-324094-23kzr8rq	195	24	range	range	NN
cord-324094-23kzr8rq	195	25	of	of	IN
cord-324094-23kzr8rq	195	26	clinical	clinical	JJ
cord-324094-23kzr8rq	195	27	diagnostic	diagnostic	JJ
cord-324094-23kzr8rq	195	28	tests	test	NNS
cord-324094-23kzr8rq	195	29	for	for	IN
cord-324094-23kzr8rq	195	30	various	various	JJ
cord-324094-23kzr8rq	195	31	infectious	infectious	JJ
cord-324094-23kzr8rq	195	32	agents	agent	NNS
cord-324094-23kzr8rq	195	33	,	,	,
cord-324094-23kzr8rq	195	34	including	include	VBG
cord-324094-23kzr8rq	195	35	viruses	virus	NNS
cord-324094-23kzr8rq	195	36	and	and	CC
cord-324094-23kzr8rq	195	37	bacteria	bacteria	NNS
cord-324094-23kzr8rq	195	38	.	.	.
cord-324094-23kzr8rq	196	1	These	these	DT
cord-324094-23kzr8rq	196	2	gene	gene	NN
cord-324094-23kzr8rq	196	3	based	base	VBN
cord-324094-23kzr8rq	196	4	tests	test	NNS
cord-324094-23kzr8rq	196	5	have	have	VBP
cord-324094-23kzr8rq	196	6	several	several	JJ
cord-324094-23kzr8rq	196	7	advantages	advantage	NNS
cord-324094-23kzr8rq	196	8	over	over	IN
cord-324094-23kzr8rq	196	9	traditional	traditional	JJ
cord-324094-23kzr8rq	196	10	antibodybased	antibodybase	VBN
cord-324094-23kzr8rq	196	11	diagnostic	diagnostic	JJ
cord-324094-23kzr8rq	196	12	methods	method	NNS
cord-324094-23kzr8rq	196	13	that	that	WDT
cord-324094-23kzr8rq	196	14	measure	measure	VBP
cord-324094-23kzr8rq	196	15	the	the	DT
cord-324094-23kzr8rq	196	16	body	body	NN
cord-324094-23kzr8rq	196	17	's	's	POS
cord-324094-23kzr8rq	196	18	immune	immune	JJ
cord-324094-23kzr8rq	196	19	response	response	NN
cord-324094-23kzr8rq	196	20	to	to	IN
cord-324094-23kzr8rq	196	21	a	a	DT
cord-324094-23kzr8rq	196	22	pathogen	pathogen	NN
cord-324094-23kzr8rq	196	23	.	.	.
cord-324094-23kzr8rq	197	1	In	in	IN
cord-324094-23kzr8rq	197	2	particular	particular	JJ
cord-324094-23kzr8rq	197	3	,	,	,
cord-324094-23kzr8rq	197	4	LAMP	LAMP	NNP
cord-324094-23kzr8rq	197	5	is	be	VBZ
cord-324094-23kzr8rq	197	6	capable	capable	JJ
cord-324094-23kzr8rq	197	7	of	of	IN
cord-324094-23kzr8rq	197	8	detecting	detect	VBG
cord-324094-23kzr8rq	197	9	the	the	DT
cord-324094-23kzr8rq	197	10	presence	presence	NN
cord-324094-23kzr8rq	197	11	of	of	IN
cord-324094-23kzr8rq	197	12	pathogenic	pathogenic	JJ
cord-324094-23kzr8rq	197	13	agents	agent	NNS
cord-324094-23kzr8rq	197	14	earlier	early	RBR
cord-324094-23kzr8rq	197	15	than	than	IN
cord-324094-23kzr8rq	197	16	PCR	PCR	NNP
cord-324094-23kzr8rq	197	17	even	even	RB
cord-324094-23kzr8rq	197	18	on	on	IN
cord-324094-23kzr8rq	197	19	day	day	NN
cord-324094-23kzr8rq	197	20	one	one	CD
cord-324094-23kzr8rq	197	21	of	of	IN
cord-324094-23kzr8rq	197	22	fever	fever	NN
cord-324094-23kzr8rq	197	23	where	where	WRB
cord-324094-23kzr8rq	197	24	the	the	DT
cord-324094-23kzr8rq	197	25	amount	amount	NN
cord-324094-23kzr8rq	197	26	of	of	IN
cord-324094-23kzr8rq	197	27	gene	gene	NN
cord-324094-23kzr8rq	197	28	copy	copy	NN
cord-324094-23kzr8rq	197	29	number	number	NN
cord-324094-23kzr8rq	197	30	is	be	VBZ
cord-324094-23kzr8rq	197	31	expected	expect	VBN
cord-324094-23kzr8rq	197	32	to	to	TO
cord-324094-23kzr8rq	197	33	be	be	VB
cord-324094-23kzr8rq	197	34	very	very	RB
cord-324094-23kzr8rq	197	35	low	low	JJ
cord-324094-23kzr8rq	197	36	due	due	IN
cord-324094-23kzr8rq	197	37	to	to	IN
cord-324094-23kzr8rq	197	38	higher	high	JJR
cord-324094-23kzr8rq	197	39	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	197	40	with	with	IN
cord-324094-23kzr8rq	197	41	a	a	DT
cord-324094-23kzr8rq	197	42	detection	detection	NN
cord-324094-23kzr8rq	197	43	limit	limit	NN
cord-324094-23kzr8rq	197	44	of	of	IN
cord-324094-23kzr8rq	197	45	about	about	RB
cord-324094-23kzr8rq	197	46	1	1	CD
cord-324094-23kzr8rq	197	47	-	-	SYM
cord-324094-23kzr8rq	197	48	2	2	CD
cord-324094-23kzr8rq	197	49	copies	copy	NNS
cord-324094-23kzr8rq	197	50	.	.	.
cord-324094-23kzr8rq	198	1	Earlier	early	JJR
cord-324094-23kzr8rq	198	2	detection	detection	NN
cord-324094-23kzr8rq	198	3	of	of	IN
cord-324094-23kzr8rq	198	4	infection	infection	NN
cord-324094-23kzr8rq	198	5	can	can	MD
cord-324094-23kzr8rq	198	6	mean	mean	VB
cord-324094-23kzr8rq	198	7	earlier	early	JJR
cord-324094-23kzr8rq	198	8	treatment	treatment	NN
cord-324094-23kzr8rq	198	9	and	and	CC
cord-324094-23kzr8rq	198	10	an	an	DT
cord-324094-23kzr8rq	198	11	earlier	early	JJR
cord-324094-23kzr8rq	198	12	return	return	NN
cord-324094-23kzr8rq	198	13	to	to	IN
cord-324094-23kzr8rq	198	14	good	good	JJ
cord-324094-23kzr8rq	198	15	health	health	NN
cord-324094-23kzr8rq	198	16	.	.	.
cord-324094-23kzr8rq	199	1	The	the	DT
cord-324094-23kzr8rq	199	2	loop	loop	NN
cord-324094-23kzr8rq	199	3	mediated	mediate	VBN
cord-324094-23kzr8rq	199	4	isothermal	isothermal	JJ
cord-324094-23kzr8rq	199	5	amplifi	amplifi	NNP
cord-324094-23kzr8rq	199	6	cation	cation	NN
cord-324094-23kzr8rq	199	7	(	(	-LRB-
cord-324094-23kzr8rq	199	8	LAMP	LAMP	NNP
cord-324094-23kzr8rq	199	9	)	)	-RRB-
cord-324094-23kzr8rq	199	10	assay	assay	NN
cord-324094-23kzr8rq	199	11	is	be	VBZ
cord-324094-23kzr8rq	199	12	emerging	emerge	VBG
cord-324094-23kzr8rq	199	13	as	as	IN
cord-324094-23kzr8rq	199	14	a	a	DT
cord-324094-23kzr8rq	199	15	simple	simple	JJ
cord-324094-23kzr8rq	199	16	,	,	,
cord-324094-23kzr8rq	199	17	rapid	rapid	JJ
cord-324094-23kzr8rq	199	18	and	and	CC
cord-324094-23kzr8rq	199	19	powerful	powerful	JJ
cord-324094-23kzr8rq	199	20	gene	gene	NN
cord-324094-23kzr8rq	199	21	amplifi	amplifi	NNP
cord-324094-23kzr8rq	199	22	cation	cation	NN
cord-324094-23kzr8rq	199	23	technique	technique	NN
cord-324094-23kzr8rq	199	24	for	for	IN
cord-324094-23kzr8rq	199	25	early	early	JJ
cord-324094-23kzr8rq	199	26	detection	detection	NN
cord-324094-23kzr8rq	199	27	of	of	IN
cord-324094-23kzr8rq	199	28	microbial	microbial	JJ
cord-324094-23kzr8rq	199	29	diseases	disease	NNS
cord-324094-23kzr8rq	199	30	.	.	.
cord-324094-23kzr8rq	200	1	Although	although	IN
cord-324094-23kzr8rq	200	2	the	the	DT
cord-324094-23kzr8rq	200	3	inception	inception	NN
cord-324094-23kzr8rq	200	4	of	of	IN
cord-324094-23kzr8rq	200	5	LAMP	LAMP	NNP
cord-324094-23kzr8rq	200	6	refers	refer	VBZ
cord-324094-23kzr8rq	200	7	back	back	RB
cord-324094-23kzr8rq	200	8	to	to	IN
cord-324094-23kzr8rq	200	9	1998	1998	CD
cord-324094-23kzr8rq	200	10	but	but	CC
cord-324094-23kzr8rq	200	11	the	the	DT
cord-324094-23kzr8rq	200	12	popularity	popularity	NN
cord-324094-23kzr8rq	200	13	of	of	IN
cord-324094-23kzr8rq	200	14	LAMP	LAMP	NNP
cord-324094-23kzr8rq	200	15	starts	start	VBZ
cord-324094-23kzr8rq	200	16	only	only	RB
cord-324094-23kzr8rq	200	17	after	after	IN
cord-324094-23kzr8rq	200	18	2003	2003	CD
cord-324094-23kzr8rq	200	19	following	follow	VBG
cord-324094-23kzr8rq	200	20	emergence	emergence	NN
cord-324094-23kzr8rq	200	21	of	of	IN
cord-324094-23kzr8rq	200	22	West	West	NNP
cord-324094-23kzr8rq	200	23	Nile	Nile	NNP
cord-324094-23kzr8rq	200	24	and	and	CC
cord-324094-23kzr8rq	200	25	SARS	SARS	NNP
cord-324094-23kzr8rq	200	26	viruses	virus	NNS
cord-324094-23kzr8rq	200	27	.	.	.
cord-324094-23kzr8rq	201	1	Since	since	IN
cord-324094-23kzr8rq	201	2	then	then	RB
cord-324094-23kzr8rq	201	3	,	,	,
cord-324094-23kzr8rq	201	4	LAMP	lamp	NN
cord-324094-23kzr8rq	201	5	assay	assay	NN
cord-324094-23kzr8rq	201	6	is	be	VBZ
cord-324094-23kzr8rq	201	7	increasingly	increasingly	RB
cord-324094-23kzr8rq	201	8	being	be	VBG
cord-324094-23kzr8rq	201	9	adapted	adapt	VBN
cord-324094-23kzr8rq	201	10	by	by	IN
cord-324094-23kzr8rq	201	11	researchers	researcher	NNS
cord-324094-23kzr8rq	201	12	mostly	mostly	RB
cord-324094-23kzr8rq	201	13	from	from	IN
cord-324094-23kzr8rq	201	14	Japan	Japan	NNP
cord-324094-23kzr8rq	201	15	in	in	IN
cord-324094-23kzr8rq	201	16	clinical	clinical	JJ
cord-324094-23kzr8rq	201	17	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	201	18	of	of	IN
cord-324094-23kzr8rq	201	19	emerging	emerge	VBG
cord-324094-23kzr8rq	201	20	diseases	disease	NNS
cord-324094-23kzr8rq	201	21	including	include	VBG
cord-324094-23kzr8rq	201	22	bacteria	bacteria	NNS
cord-324094-23kzr8rq	201	23	,	,	,
cord-324094-23kzr8rq	201	24	viruses	virus	NNS
cord-324094-23kzr8rq	201	25	and	and	CC
cord-324094-23kzr8rq	201	26	parasitic	parasitic	JJ
cord-324094-23kzr8rq	201	27	diseases	disease	NNS
cord-324094-23kzr8rq	201	28	.	.	.
cord-324094-23kzr8rq	202	1	LAMP	LAMP	NNP
cord-324094-23kzr8rq	202	2	has	have	VBZ
cord-324094-23kzr8rq	202	3	been	be	VBN
cord-324094-23kzr8rq	202	4	successfully	successfully	RB
cord-324094-23kzr8rq	202	5	applied	apply	VBN
cord-324094-23kzr8rq	202	6	for	for	IN
cord-324094-23kzr8rq	202	7	rapid	rapid	JJ
cord-324094-23kzr8rq	202	8	and	and	CC
cord-324094-23kzr8rq	202	9	real	real	JJ
cord-324094-23kzr8rq	202	10	-	-	HYPH
cord-324094-23kzr8rq	202	11	time	time	NN
cord-324094-23kzr8rq	202	12	detection	detection	NN
cord-324094-23kzr8rq	202	13	of	of	IN
cord-324094-23kzr8rq	202	14	both	both	DT
cord-324094-23kzr8rq	202	15	DNA	DNA	NNP
cord-324094-23kzr8rq	202	16	and	and	CC
cord-324094-23kzr8rq	202	17	RNA	RNA	NNP
cord-324094-23kzr8rq	202	18	viruses	virus	NNS
cord-324094-23kzr8rq	202	19	.	.	.
cord-324094-23kzr8rq	203	1	However	however	RB
cord-324094-23kzr8rq	203	2	,	,	,
cord-324094-23kzr8rq	203	3	most	most	JJS
cord-324094-23kzr8rq	203	4	of	of	IN
cord-324094-23kzr8rq	203	5	the	the	DT
cord-324094-23kzr8rq	203	6	published	publish	VBN
cord-324094-23kzr8rq	203	7	researches	research	NNS
cord-324094-23kzr8rq	203	8	have	have	VBP
cord-324094-23kzr8rq	203	9	been	be	VBN
cord-324094-23kzr8rq	203	10	directed	direct	VBN
cord-324094-23kzr8rq	203	11	for	for	IN
cord-324094-23kzr8rq	203	12	RNA	RNA	NNP
cord-324094-23kzr8rq	203	13	viruses	virus	NNS
cord-324094-23kzr8rq	203	14	may	may	MD
cord-324094-23kzr8rq	203	15	be	be	VB
cord-324094-23kzr8rq	203	16	due	due	JJ
cord-324094-23kzr8rq	203	17	to	to	IN
cord-324094-23kzr8rq	203	18	the	the	DT
cord-324094-23kzr8rq	203	19	increased	increase	VBN
cord-324094-23kzr8rq	203	20	incidence	incidence	NN
cord-324094-23kzr8rq	203	21	of	of	IN
cord-324094-23kzr8rq	203	22	RNA	RNA	NNP
cord-324094-23kzr8rq	203	23	viruses	virus	NNS
cord-324094-23kzr8rq	203	24	in	in	IN
cord-324094-23kzr8rq	203	25	recent	recent	JJ
cord-324094-23kzr8rq	203	26	past	past	NN
cord-324094-23kzr8rq	203	27	in	in	IN
cord-324094-23kzr8rq	203	28	the	the	DT
cord-324094-23kzr8rq	203	29	form	form	NN
cord-324094-23kzr8rq	203	30	of	of	IN
cord-324094-23kzr8rq	203	31	major	major	JJ
cord-324094-23kzr8rq	203	32	epidemic	epidemic	NN
cord-324094-23kzr8rq	203	33	having	have	VBG
cord-324094-23kzr8rq	203	34	signifi	signifi	NNP
cord-324094-23kzr8rq	204	1	ca	can	MD
cord-324094-23kzr8rq	204	2	nt	not	RB
cord-324094-23kzr8rq	204	3	public	public	JJ
cord-324094-23kzr8rq	204	4	health	health	NN
cord-324094-23kzr8rq	204	5	importance	importance	NN
cord-324094-23kzr8rq	204	6	.	.	.
cord-324094-23kzr8rq	205	1	A	a	DT
cord-324094-23kzr8rq	205	2	one	one	CD
cord-324094-23kzr8rq	205	3	-	-	HYPH
cord-324094-23kzr8rq	205	4	step	step	NN
cord-324094-23kzr8rq	205	5	single	single	JJ
cord-324094-23kzr8rq	205	6	tube	tube	NN
cord-324094-23kzr8rq	205	7	real	real	JJ
cord-324094-23kzr8rq	205	8	-	-	HYPH
cord-324094-23kzr8rq	205	9	time	time	NN
cord-324094-23kzr8rq	205	10	accelerated	accelerate	VBN
cord-324094-23kzr8rq	205	11	reverse	reverse	NN
cord-324094-23kzr8rq	205	12	transcription	transcription	NN
cord-324094-23kzr8rq	205	13	loop	loop	NN
cord-324094-23kzr8rq	205	14	mediated	mediate	VBN
cord-324094-23kzr8rq	205	15	isothermal	isothermal	JJ
cord-324094-23kzr8rq	205	16	amplifi	amplifi	NNP
cord-324094-23kzr8rq	205	17	cation	cation	NN
cord-324094-23kzr8rq	205	18	(	(	-LRB-
cord-324094-23kzr8rq	205	19	RT	RT	NNP
cord-324094-23kzr8rq	205	20	-	-	HYPH
cord-324094-23kzr8rq	205	21	LAMP	LAMP	NNP
cord-324094-23kzr8rq	205	22	)	)	-RRB-
cord-324094-23kzr8rq	205	23	assays	assay	NNS
cord-324094-23kzr8rq	205	24	for	for	IN
cord-324094-23kzr8rq	205	25	rapid	rapid	JJ
cord-324094-23kzr8rq	205	26	detection	detection	NN
cord-324094-23kzr8rq	205	27	of	of	IN
cord-324094-23kzr8rq	205	28	some	some	DT
cord-324094-23kzr8rq	205	29	of	of	IN
cord-324094-23kzr8rq	205	30	the	the	DT
cord-324094-23kzr8rq	205	31	recently	recently	RB
cord-324094-23kzr8rq	205	32	emerged	emerge	VBN
cord-324094-23kzr8rq	205	33	human	human	JJ
cord-324094-23kzr8rq	205	34	viral	viral	JJ
cord-324094-23kzr8rq	205	35	pathogens	pathogen	NNS
cord-324094-23kzr8rq	205	36	viz	viz	JJ
cord-324094-23kzr8rq	205	37	.	.	.
cord-324094-23kzr8rq	206	1	West	West	NNP
cord-324094-23kzr8rq	206	2	Nile	Nile	NNP
cord-324094-23kzr8rq	206	3	,	,	,
cord-324094-23kzr8rq	206	4	SARS	SARS	NNP
cord-324094-23kzr8rq	206	5	,	,	,
cord-324094-23kzr8rq	206	6	dengue	dengue	NN
cord-324094-23kzr8rq	206	7	,	,	,
cord-324094-23kzr8rq	206	8	Japanese	japanese	JJ
cord-324094-23kzr8rq	206	9	encephalitis	encephalitis	RB
cord-324094-23kzr8rq	206	10	,	,	,
cord-324094-23kzr8rq	206	11	chikungunya	chikungunya	NN
cord-324094-23kzr8rq	206	12	,	,	,
cord-324094-23kzr8rq	206	13	Norwalk	Norwalk	NNP
cord-324094-23kzr8rq	206	14	,	,	,
cord-324094-23kzr8rq	206	15	H5N1	H5N1	NNP
cord-324094-23kzr8rq	206	16	highly	highly	RB
cord-324094-23kzr8rq	206	17	pathogenic	pathogenic	JJ
cord-324094-23kzr8rq	206	18	avian	avian	JJ
cord-324094-23kzr8rq	206	19	infl	infl	NN
cord-324094-23kzr8rq	206	20	uenza	uenza	NN
cord-324094-23kzr8rq	206	21	(	(	-LRB-
cord-324094-23kzr8rq	206	22	HPAI	HPAI	NNP
cord-324094-23kzr8rq	206	23	)	)	-RRB-
cord-324094-23kzr8rq	206	24	viruses	virus	NNS
cord-324094-23kzr8rq	206	25	have	have	VBP
cord-324094-23kzr8rq	206	26	been	be	VBN
cord-324094-23kzr8rq	206	27	developed	develop	VBN
cord-324094-23kzr8rq	206	28	and	and	CC
cord-324094-23kzr8rq	206	29	evaluated	evaluate	VBN
cord-324094-23kzr8rq	207	1	(	(	-LRB-
cord-324094-23kzr8rq	207	2	Parida	Parida	NNP
cord-324094-23kzr8rq	207	3	et	et	NNP
cord-324094-23kzr8rq	207	4	al	al	NNP
cord-324094-23kzr8rq	207	5	2004	2004	CD
cord-324094-23kzr8rq	208	1	(	(	-LRB-
cord-324094-23kzr8rq	208	2	Parida	Parida	NNP
cord-324094-23kzr8rq	208	3	et	et	NNP
cord-324094-23kzr8rq	208	4	al	al	NNP
cord-324094-23kzr8rq	208	5	,	,	,
cord-324094-23kzr8rq	208	6	2005	2005	CD
cord-324094-23kzr8rq	209	1	(	(	-LRB-
cord-324094-23kzr8rq	209	2	Parida	Parida	NNP
cord-324094-23kzr8rq	209	3	et	et	NNP
cord-324094-23kzr8rq	209	4	al	al	NNP
cord-324094-23kzr8rq	209	5	,	,	,
cord-324094-23kzr8rq	209	6	2006	2006	CD
cord-324094-23kzr8rq	210	1	(	(	-LRB-
cord-324094-23kzr8rq	210	2	Parida	Parida	NNP
cord-324094-23kzr8rq	210	3	et	et	NNP
cord-324094-23kzr8rq	210	4	al	al	NNP
cord-324094-23kzr8rq	210	5	,	,	,
cord-324094-23kzr8rq	210	6	2008	2008	CD
cord-324094-23kzr8rq	211	1	Hong	Hong	NNP
cord-324094-23kzr8rq	211	2	et	et	NNP
cord-324094-23kzr8rq	211	3	al	al	NNP
cord-324094-23kzr8rq	211	4	2005	2005	CD
cord-324094-23kzr8rq	211	5	;	;	:
cord-324094-23kzr8rq	211	6	Imai	Imai	NNP
cord-324094-23kzr8rq	211	7	et	et	NNP
cord-324094-23kzr8rq	211	8	al	al	NNP
cord-324094-23kzr8rq	211	9	2006	2006	CD
cord-324094-23kzr8rq	211	10	;	;	:
cord-324094-23kzr8rq	212	1	Toriniwa	Toriniwa	NNP
cord-324094-23kzr8rq	212	2	et	et	NNP
cord-324094-23kzr8rq	212	3	al	al	NNP
cord-324094-23kzr8rq	212	4	2006	2006	CD
cord-324094-23kzr8rq	212	5	)	)	-RRB-
cord-324094-23kzr8rq	213	1	On	on	IN
cord-324094-23kzr8rq	213	2	comparison	comparison	NN
cord-324094-23kzr8rq	213	3	to	to	IN
cord-324094-23kzr8rq	213	4	conventional	conventional	JJ
cord-324094-23kzr8rq	213	5	RT	RT	NNP
cord-324094-23kzr8rq	213	6	-	-	HYPH
cord-324094-23kzr8rq	213	7	PCR	PCR	NNP
cord-324094-23kzr8rq	213	8	,	,	,
cord-324094-23kzr8rq	213	9	RT	RT	NNP
cord-324094-23kzr8rq	213	10	-	-	HYPH
cord-324094-23kzr8rq	213	11	LAMP	LAMP	NNP
cord-324094-23kzr8rq	213	12	assay	assay	NN
cord-324094-23kzr8rq	213	13	demonstrated	demonstrate	VBD
cord-324094-23kzr8rq	213	14	10	10	CD
cord-324094-23kzr8rq	213	15	to	to	TO
cord-324094-23kzr8rq	213	16	100	100	CD
cord-324094-23kzr8rq	213	17	fold	fold	VB
cord-324094-23kzr8rq	213	18	more	more	JJR
cord-324094-23kzr8rq	213	19	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	213	20	with	with	IN
cord-324094-23kzr8rq	213	21	a	a	DT
cord-324094-23kzr8rq	213	22	detection	detection	NN
cord-324094-23kzr8rq	213	23	limit	limit	NN
cord-324094-23kzr8rq	213	24	of	of	IN
cord-324094-23kzr8rq	213	25	0.01	0.01	CD
cord-324094-23kzr8rq	213	26	to	to	TO
cord-324094-23kzr8rq	213	27	10	10	CD
cord-324094-23kzr8rq	213	28	PFU	pfu	NN
cord-324094-23kzr8rq	213	29	of	of	IN
cord-324094-23kzr8rq	213	30	virus	virus	NN
cord-324094-23kzr8rq	213	31	in	in	IN
cord-324094-23kzr8rq	213	32	all	all	PDT
cord-324094-23kzr8rq	213	33	these	these	DT
cord-324094-23kzr8rq	213	34	cases	case	NNS
cord-324094-23kzr8rq	213	35	.	.	.
cord-324094-23kzr8rq	214	1	The	the	DT
cord-324094-23kzr8rq	214	2	usefulness	usefulness	NN
cord-324094-23kzr8rq	214	3	of	of	IN
cord-324094-23kzr8rq	214	4	LAMP	lamp	NN
cord-324094-23kzr8rq	214	5	for	for	IN
cord-324094-23kzr8rq	214	6	amplifi	amplifi	NNP
cord-324094-23kzr8rq	214	7	cation	cation	NN
cord-324094-23kzr8rq	214	8	of	of	IN
cord-324094-23kzr8rq	214	9	DNA	DNA	NNP
cord-324094-23kzr8rq	214	10	viruses	virus	NNS
cord-324094-23kzr8rq	214	11	was	be	VBD
cord-324094-23kzr8rq	214	12	also	also	RB
cord-324094-23kzr8rq	214	13	reported	report	VBN
cord-324094-23kzr8rq	214	14	for	for	IN
cord-324094-23kzr8rq	214	15	HBV	HBV	NNP
cord-324094-23kzr8rq	214	16	,	,	,
cord-324094-23kzr8rq	214	17	HPV	HPV	NNP
cord-324094-23kzr8rq	214	18	(	(	-LRB-
cord-324094-23kzr8rq	214	19	human	human	JJ
cord-324094-23kzr8rq	214	20	papillomavirus	papillomavirus	NN
cord-324094-23kzr8rq	214	21	)	)	-RRB-
cord-324094-23kzr8rq	214	22	type	type	NN
cord-324094-23kzr8rq	214	23	-6,11	-6,11	:
cord-324094-23kzr8rq	214	24	,	,	,
cord-324094-23kzr8rq	214	25	16	16	CD
cord-324094-23kzr8rq	214	26	and	and	CC
cord-324094-23kzr8rq	214	27	18	18	CD
cord-324094-23kzr8rq	214	28	,	,	,
cord-324094-23kzr8rq	214	29	HSV	HSV	NNP
cord-324094-23kzr8rq	214	30	,	,	,
cord-324094-23kzr8rq	214	31	varivella	varivella	NNP
cord-324094-23kzr8rq	214	32	zooster	zooster	NNP
cord-324094-23kzr8rq	214	33	virus	virus	NN
cord-324094-23kzr8rq	214	34	(	(	-LRB-
cord-324094-23kzr8rq	214	35	VZV	VZV	NNP
cord-324094-23kzr8rq	214	36	)	)	-RRB-
cord-324094-23kzr8rq	214	37	,	,	,
cord-324094-23kzr8rq	214	38	cytomegalo	cytomegalo	NNP
cord-324094-23kzr8rq	214	39	virus	virus	NN
cord-324094-23kzr8rq	214	40	(	(	-LRB-
cord-324094-23kzr8rq	214	41	CMV	CMV	NNP
cord-324094-23kzr8rq	214	42	)	)	-RRB-
cord-324094-23kzr8rq	214	43	and	and	CC
cord-324094-23kzr8rq	214	44	found	find	VBD
cord-324094-23kzr8rq	214	45	to	to	TO
cord-324094-23kzr8rq	214	46	be	be	VB
cord-324094-23kzr8rq	214	47	superior	superior	JJ
cord-324094-23kzr8rq	214	48	in	in	IN
cord-324094-23kzr8rq	214	49	terms	term	NNS
cord-324094-23kzr8rq	214	50	of	of	IN
cord-324094-23kzr8rq	214	51	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	214	52	,	,	,
cord-324094-23kzr8rq	214	53	specifi	specifi	VBN
cord-324094-23kzr8rq	214	54	city	city	NN
cord-324094-23kzr8rq	214	55	,	,	,
cord-324094-23kzr8rq	214	56	rapidity	rapidity	NN
cord-324094-23kzr8rq	214	57	,	,	,
cord-324094-23kzr8rq	214	58	and	and	CC
cord-324094-23kzr8rq	214	59	simplicity	simplicity	NN
cord-324094-23kzr8rq	214	60	,	,	,
cord-324094-23kzr8rq	214	61	and	and	CC
cord-324094-23kzr8rq	214	62	can	can	MD
cord-324094-23kzr8rq	214	63	potentially	potentially	RB
cord-324094-23kzr8rq	214	64	be	be	VB
cord-324094-23kzr8rq	214	65	a	a	DT
cord-324094-23kzr8rq	214	66	valuable	valuable	JJ
cord-324094-23kzr8rq	214	67	tool	tool	NN
cord-324094-23kzr8rq	214	68	for	for	IN
cord-324094-23kzr8rq	214	69	the	the	DT
cord-324094-23kzr8rq	214	70	detection	detection	NN
cord-324094-23kzr8rq	214	71	of	of	IN
cord-324094-23kzr8rq	214	72	HPV	HPV	NNP
cord-324094-23kzr8rq	214	73	DNA	dna	NN
cord-324094-23kzr8rq	214	74	compared	compare	VBN
cord-324094-23kzr8rq	214	75	to	to	IN
cord-324094-23kzr8rq	214	76	PCR	PCR	NNP
cord-324094-23kzr8rq	214	77	and	and	CC
cord-324094-23kzr8rq	214	78	real	real	JJ
cord-324094-23kzr8rq	214	79	-	-	HYPH
cord-324094-23kzr8rq	214	80	time	time	NN
cord-324094-23kzr8rq	214	81	PCR	PCR	NNP
cord-324094-23kzr8rq	214	82	(	(	-LRB-
cord-324094-23kzr8rq	214	83	 	 	_SP
cord-324094-23kzr8rq	215	1	The	the	DT
cord-324094-23kzr8rq	215	2	combination	combination	NN
cord-324094-23kzr8rq	215	3	of	of	IN
cord-324094-23kzr8rq	215	4	excellent	excellent	JJ
cord-324094-23kzr8rq	215	5	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	215	6	and	and	CC
cord-324094-23kzr8rq	215	7	specifi	specifi	NN
cord-324094-23kzr8rq	215	8	city	city	NN
cord-324094-23kzr8rq	215	9	,	,	,
cord-324094-23kzr8rq	215	10	low	low	JJ
cord-324094-23kzr8rq	215	11	contamination	contamination	NN
cord-324094-23kzr8rq	215	12	risk	risk	NN
cord-324094-23kzr8rq	215	13	,	,	,
cord-324094-23kzr8rq	215	14	and	and	CC
cord-324094-23kzr8rq	215	15	speed	speed	NN
cord-324094-23kzr8rq	215	16	has	have	VBZ
cord-324094-23kzr8rq	215	17	made	make	VBN
cord-324094-23kzr8rq	215	18	real	real	JJ
cord-324094-23kzr8rq	215	19	-	-	HYPH
cord-324094-23kzr8rq	215	20	time	time	NN
cord-324094-23kzr8rq	215	21	PCR	PCR	NNP
cord-324094-23kzr8rq	215	22	technology	technology	NN
cord-324094-23kzr8rq	215	23	an	an	DT
cord-324094-23kzr8rq	215	24	appealing	appealing	JJ
cord-324094-23kzr8rq	215	25	alternative	alternative	NN
cord-324094-23kzr8rq	215	26	to	to	IN
cord-324094-23kzr8rq	215	27	culture	culture	NN
cord-324094-23kzr8rq	215	28	-	-	HYPH
cord-324094-23kzr8rq	215	29	or	or	CC
cord-324094-23kzr8rq	215	30	immunoassay	immunoassay	RB
cord-324094-23kzr8rq	215	31	-	-	HYPH
cord-324094-23kzr8rq	215	32	based	base	VBN
cord-324094-23kzr8rq	215	33	testing	testing	NN
cord-324094-23kzr8rq	215	34	methods	method	NNS
cord-324094-23kzr8rq	215	35	for	for	IN
cord-324094-23kzr8rq	215	36	diagnosing	diagnose	VBG
cord-324094-23kzr8rq	215	37	many	many	JJ
cord-324094-23kzr8rq	215	38	infectious	infectious	JJ
cord-324094-23kzr8rq	215	39	diseases	disease	NNS
cord-324094-23kzr8rq	215	40	.	.	.
cord-324094-23kzr8rq	216	1	The	the	DT
cord-324094-23kzr8rq	216	2	recent	recent	JJ
cord-324094-23kzr8rq	216	3	advances	advance	NNS
cord-324094-23kzr8rq	216	4	in	in	IN
cord-324094-23kzr8rq	216	5	the	the	DT
cord-324094-23kzr8rq	216	6	development	development	NN
cord-324094-23kzr8rq	216	7	of	of	IN
cord-324094-23kzr8rq	216	8	fl	fl	NNP
cord-324094-23kzr8rq	216	9	uorophores	uorophore	NNS
cord-324094-23kzr8rq	216	10	,	,	,
cord-324094-23kzr8rq	216	11	nucleotide	nucleotide	JJ
cord-324094-23kzr8rq	216	12	labeling	labeling	NN
cord-324094-23kzr8rq	216	13	chemistries	chemistry	NNS
cord-324094-23kzr8rq	216	14	,	,	,
cord-324094-23kzr8rq	216	15	and	and	CC
cord-324094-23kzr8rq	216	16	the	the	DT
cord-324094-23kzr8rq	216	17	novel	novel	JJ
cord-324094-23kzr8rq	216	18	applications	application	NNS
cord-324094-23kzr8rq	216	19	of	of	IN
cord-324094-23kzr8rq	216	20	oligoprobe	oligoprobe	NN
cord-324094-23kzr8rq	216	21	hybridization	hybridization	NN
cord-324094-23kzr8rq	216	22	have	have	VBP
cord-324094-23kzr8rq	216	23	provided	provide	VBN
cord-324094-23kzr8rq	216	24	real	real	JJ
cord-324094-23kzr8rq	216	25	-	-	HYPH
cord-324094-23kzr8rq	216	26	time	time	NN
cord-324094-23kzr8rq	216	27	PCR	PCR	NNP
cord-324094-23kzr8rq	216	28	technologies	technology	NNS
cord-324094-23kzr8rq	216	29	with	with	IN
cord-324094-23kzr8rq	216	30	a	a	DT
cord-324094-23kzr8rq	216	31	broad	broad	JJ
cord-324094-23kzr8rq	216	32	enough	enough	JJ
cord-324094-23kzr8rq	216	33	base	base	NN
cord-324094-23kzr8rq	216	34	to	to	TO
cord-324094-23kzr8rq	216	35	ensure	ensure	VB
cord-324094-23kzr8rq	216	36	their	-PRON-	PRP$
cord-324094-23kzr8rq	216	37	acceptance	acceptance	NN
cord-324094-23kzr8rq	216	38	.	.	.
cord-324094-23kzr8rq	217	1	The	the	DT
cord-324094-23kzr8rq	217	2	LAMP	LAMP	NNP
cord-324094-23kzr8rq	217	3	is	be	VBZ
cord-324094-23kzr8rq	217	4	emerging	emerge	VBG
cord-324094-23kzr8rq	217	5	as	as	IN
cord-324094-23kzr8rq	217	6	a	a	DT
cord-324094-23kzr8rq	217	7	new	new	JJ
cord-324094-23kzr8rq	217	8	generation	generation	NN
cord-324094-23kzr8rq	217	9	of	of	IN
cord-324094-23kzr8rq	217	10	cost	cost	NN
cord-324094-23kzr8rq	217	11	effective	effective	JJ
cord-324094-23kzr8rq	217	12	and	and	CC
cord-324094-23kzr8rq	217	13	rapid	rapid	JJ
cord-324094-23kzr8rq	217	14	gene	gene	NN
cord-324094-23kzr8rq	217	15	amplifi	amplifi	NNP
cord-324094-23kzr8rq	217	16	cation	cation	NN
cord-324094-23kzr8rq	217	17	tool	tool	NN
cord-324094-23kzr8rq	217	18	having	have	VBG
cord-324094-23kzr8rq	217	19	all	all	PDT
cord-324094-23kzr8rq	217	20	the	the	DT
cord-324094-23kzr8rq	217	21	characteristics	characteristic	NNS
cord-324094-23kzr8rq	217	22	of	of	IN
cord-324094-23kzr8rq	217	23	rapidity	rapidity	NN
cord-324094-23kzr8rq	217	24	and	and	CC
cord-324094-23kzr8rq	217	25	high	high	JJ
cord-324094-23kzr8rq	217	26	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	217	27	of	of	IN
cord-324094-23kzr8rq	217	28	real	real	JJ
cord-324094-23kzr8rq	217	29	-	-	HYPH
cord-324094-23kzr8rq	217	30	time	time	NN
cord-324094-23kzr8rq	217	31	assays	assay	NNS
cord-324094-23kzr8rq	217	32	as	as	RB
cord-324094-23kzr8rq	217	33	well	well	RB
cord-324094-23kzr8rq	217	34	as	as	IN
cord-324094-23kzr8rq	217	35	easy	easy	JJ
cord-324094-23kzr8rq	217	36	adaptability	adaptability	NN
cord-324094-23kzr8rq	217	37	under	under	IN
cord-324094-23kzr8rq	217	38	fi	fi	NNP
cord-324094-23kzr8rq	217	39	eld	eld	NN
cord-324094-23kzr8rq	217	40	conditions	condition	NNS
cord-324094-23kzr8rq	217	41	due	due	JJ
cord-324094-23kzr8rq	217	42	to	to	IN
cord-324094-23kzr8rq	217	43	its	-PRON-	PRP$
cord-324094-23kzr8rq	217	44	simple	simple	JJ
cord-324094-23kzr8rq	217	45	operation	operation	NN
cord-324094-23kzr8rq	217	46	,	,	,
cord-324094-23kzr8rq	217	47	rapid	rapid	JJ
cord-324094-23kzr8rq	217	48	reaction	reaction	NN
cord-324094-23kzr8rq	217	49	,	,	,
cord-324094-23kzr8rq	217	50	and	and	CC
cord-324094-23kzr8rq	217	51	easy	easy	JJ
cord-324094-23kzr8rq	217	52	detection	detection	NN
cord-324094-23kzr8rq	217	53	.	.	.
cord-324094-23kzr8rq	218	1	The	the	DT
cord-324094-23kzr8rq	218	2	rapidity	rapidity	NN
cord-324094-23kzr8rq	218	3	and	and	CC
cord-324094-23kzr8rq	218	4	sensitivity	sensitivity	NN
cord-324094-23kzr8rq	218	5	of	of	IN
cord-324094-23kzr8rq	218	6	these	these	DT
cord-324094-23kzr8rq	218	7	real	real	JJ
cord-324094-23kzr8rq	218	8	-	-	HYPH
cord-324094-23kzr8rq	218	9	time	time	NN
cord-324094-23kzr8rq	218	10	assays	assay	NNS
cord-324094-23kzr8rq	218	11	will	will	MD
cord-324094-23kzr8rq	218	12	assist	assist	VB
cord-324094-23kzr8rq	218	13	in	in	IN
cord-324094-23kzr8rq	218	14	precise	precise	JJ
cord-324094-23kzr8rq	218	15	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	218	16	,	,	,
cord-324094-23kzr8rq	218	17	which	which	WDT
cord-324094-23kzr8rq	218	18	is	be	VBZ
cord-324094-23kzr8rq	218	19	extremely	extremely	RB
cord-324094-23kzr8rq	218	20	useful	useful	JJ
cord-324094-23kzr8rq	218	21	to	to	TO
cord-324094-23kzr8rq	218	22	undertake	undertake	VB
cord-324094-23kzr8rq	218	23	suitable	suitable	JJ
cord-324094-23kzr8rq	218	24	control	control	NN
cord-324094-23kzr8rq	218	25	measures	measure	NNS
cord-324094-23kzr8rq	218	26	and	and	CC
cord-324094-23kzr8rq	218	27	patient	patient	NN
cord-324094-23kzr8rq	218	28	management	management	NN
cord-324094-23kzr8rq	218	29	at	at	IN
cord-324094-23kzr8rq	218	30	the	the	DT
cord-324094-23kzr8rq	218	31	earliest	early	JJS
cord-324094-23kzr8rq	218	32	.	.	.
cord-324094-23kzr8rq	219	1	Simultaneous	simultaneous	JJ
cord-324094-23kzr8rq	219	2	detection	detection	NN
cord-324094-23kzr8rq	219	3	of	of	IN
cord-324094-23kzr8rq	219	4	enteric	enteric	JJ
cord-324094-23kzr8rq	219	5	viruses	virus	NNS
cord-324094-23kzr8rq	219	6	by	by	IN
cord-324094-23kzr8rq	219	7	multiplex	multiplex	JJ
cord-324094-23kzr8rq	219	8	real	real	JJ
cord-324094-23kzr8rq	219	9	-	-	HYPH
cord-324094-23kzr8rq	219	10	time	time	NN
cord-324094-23kzr8rq	219	11	RT	rt	NN
cord-324094-23kzr8rq	219	12	-	-	HYPH
cord-324094-23kzr8rq	219	13	PCR	PCR	NNP
cord-324094-23kzr8rq	219	14	NASBA	NASBA	NNP
cord-324094-23kzr8rq	219	15	and	and	CC
cord-324094-23kzr8rq	219	16	other	other	JJ
cord-324094-23kzr8rq	219	17	transcription	transcription	NN
cord-324094-23kzr8rq	219	18	-	-	HYPH
cord-324094-23kzr8rq	219	19	based	base	VBN
cord-324094-23kzr8rq	219	20	amplifi	amplifi	NNP
cord-324094-23kzr8rq	219	21	cation	cation	NN
cord-324094-23kzr8rq	219	22	methods	method	NNS
cord-324094-23kzr8rq	219	23	for	for	IN
cord-324094-23kzr8rq	219	24	research	research	NN
cord-324094-23kzr8rq	219	25	and	and	CC
cord-324094-23kzr8rq	219	26	diagnostic	diagnostic	JJ
cord-324094-23kzr8rq	219	27	microbiology	microbiology	NN
cord-324094-23kzr8rq	219	28	Development	Development	NNP
cord-324094-23kzr8rq	219	29	of	of	IN
cord-324094-23kzr8rq	219	30	Real	Real	NNP
cord-324094-23kzr8rq	219	31	-	-	HYPH
cord-324094-23kzr8rq	219	32	Time	time	NN
cord-324094-23kzr8rq	219	33	Reverse	Reverse	NNP
cord-324094-23kzr8rq	219	34	Transcriptase	Transcriptase	NNP
cord-324094-23kzr8rq	219	35	PCR	pcr	NN
cord-324094-23kzr8rq	219	36	Assay	assay	NN
cord-324094-23kzr8rq	220	1	To	to	TO
cord-324094-23kzr8rq	220	2	Detect	detect	VB
cord-324094-23kzr8rq	220	3	and	and	CC
cord-324094-23kzr8rq	220	4	Serotype	Serotype	NNP
cord-324094-23kzr8rq	220	5	Dengue	Dengue	NNP
cord-324094-23kzr8rq	220	6	Viruses	Viruses	NNPS
cord-324094-23kzr8rq	220	7	TaqMan	TaqMan	NNP
cord-324094-23kzr8rq	220	8	reverse	reverse	JJ
cord-324094-23kzr8rq	220	9	transcription	transcription	NN
cord-324094-23kzr8rq	220	10	polymerase	polymerase	NN
cord-324094-23kzr8rq	220	11	chain	chain	NN
cord-324094-23kzr8rq	220	12	reaction	reaction	NN
cord-324094-23kzr8rq	220	13	for	for	IN
cord-324094-23kzr8rq	220	14	the	the	DT
cord-324094-23kzr8rq	220	15	detection	detection	NN
cord-324094-23kzr8rq	220	16	of	of	IN
cord-324094-23kzr8rq	220	17	Japanese	japanese	JJ
cord-324094-23kzr8rq	220	18	encephalitis	encephalitis	RB
cord-324094-23kzr8rq	220	19	virus	virus	NN
cord-324094-23kzr8rq	220	20	Infl	Infl	NNP
cord-324094-23kzr8rq	220	21	uenza	uenza	NNP
cord-324094-23kzr8rq	221	1	A	a	DT
cord-324094-23kzr8rq	221	2	H5N1	H5N1	NNP
cord-324094-23kzr8rq	221	3	Detection	Detection	NNP
cord-324094-23kzr8rq	221	4	Rapid	Rapid	NNP
cord-324094-23kzr8rq	221	5	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	221	6	of	of	IN
cord-324094-23kzr8rq	221	7	herpes	herpes	NNP
cord-324094-23kzr8rq	221	8	simplex	simplex	NNP
cord-324094-23kzr8rq	221	9	virus	virus	NN
cord-324094-23kzr8rq	221	10	infection	infection	NN
cord-324094-23kzr8rq	221	11	by	by	IN
cord-324094-23kzr8rq	221	12	a	a	DT
cord-324094-23kzr8rq	221	13	loop	loop	NN
cord-324094-23kzr8rq	221	14	-	-	HYPH
cord-324094-23kzr8rq	221	15	mediated	mediate	VBN
cord-324094-23kzr8rq	221	16	isothermal	isothermal	JJ
cord-324094-23kzr8rq	221	17	amplifi	amplifi	NNP
cord-324094-23kzr8rq	221	18	cation	cation	NN
cord-324094-23kzr8rq	221	19	method	method	NN
cord-324094-23kzr8rq	221	20	Real	real	JJ
cord-324094-23kzr8rq	221	21	-	-	HYPH
cord-324094-23kzr8rq	221	22	time	time	NN
cord-324094-23kzr8rq	221	23	PCR	PCR	NNP
cord-324094-23kzr8rq	221	24	in	in	IN
cord-324094-23kzr8rq	221	25	clinical	clinical	JJ
cord-324094-23kzr8rq	221	26	microbiology	microbiology	NN
cord-324094-23kzr8rq	221	27	:	:	:
cord-324094-23kzr8rq	221	28	applications	application	NNS
cord-324094-23kzr8rq	221	29	for	for	IN
cord-324094-23kzr8rq	221	30	routine	routine	JJ
cord-324094-23kzr8rq	221	31	laboratory	laboratory	NN
cord-324094-23kzr8rq	221	32	testing	test	VBG
cord-324094-23kzr8rq	221	33	Rapid	rapid	JJ
cord-324094-23kzr8rq	221	34	detection	detection	NN
cord-324094-23kzr8rq	221	35	of	of	IN
cord-324094-23kzr8rq	221	36	norovirus	norovirus	NN
cord-324094-23kzr8rq	221	37	from	from	IN
cord-324094-23kzr8rq	221	38	fecal	fecal	JJ
cord-324094-23kzr8rq	221	39	specimens	specimen	NNS
cord-324094-23kzr8rq	221	40	by	by	IN
cord-324094-23kzr8rq	221	41	real	real	JJ
cord-324094-23kzr8rq	221	42	-	-	HYPH
cord-324094-23kzr8rq	221	43	time	time	NN
cord-324094-23kzr8rq	221	44	reverse	reverse	JJ
cord-324094-23kzr8rq	221	45	transcription	transcription	NN
cord-324094-23kzr8rq	221	46	-	-	HYPH
cord-324094-23kzr8rq	221	47	loop	loop	NN
cord-324094-23kzr8rq	221	48	-	-	HYPH
cord-324094-23kzr8rq	221	49	mediated	mediate	VBN
cord-324094-23kzr8rq	221	50	isothermal	isothermal	JJ
cord-324094-23kzr8rq	221	51	amplifi	amplifi	NNP
cord-324094-23kzr8rq	221	52	cation	cation	NN
cord-324094-23kzr8rq	221	53	assay	assay	NN
cord-324094-23kzr8rq	221	54	Practical	practical	JJ
cord-324094-23kzr8rq	221	55	experience	experience	NN
cord-324094-23kzr8rq	221	56	of	of	IN
cord-324094-23kzr8rq	221	57	high	high	JJ
cord-324094-23kzr8rq	221	58	throughput	throughput	NN
cord-324094-23kzr8rq	222	1	real	real	JJ
cord-324094-23kzr8rq	222	2	time	time	NN
cord-324094-23kzr8rq	222	3	PCR	PCR	NNP
cord-324094-23kzr8rq	222	4	in	in	IN
cord-324094-23kzr8rq	222	5	the	the	DT
cord-324094-23kzr8rq	222	6	routine	routine	JJ
cord-324094-23kzr8rq	222	7	diagnostic	diagnostic	JJ
cord-324094-23kzr8rq	222	8	virology	virology	NN
cord-324094-23kzr8rq	222	9	setting	set	VBG
cord-324094-23kzr8rq	222	10	Loop	loop	NN
cord-324094-23kzr8rq	222	11	-	-	HYPH
cord-324094-23kzr8rq	222	12	mediated	mediate	VBN
cord-324094-23kzr8rq	222	13	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	14	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	15	cation	cation	NN
cord-324094-23kzr8rq	222	16	method	method	NN
cord-324094-23kzr8rq	222	17	for	for	IN
cord-324094-23kzr8rq	222	18	detection	detection	NN
cord-324094-23kzr8rq	222	19	of	of	IN
cord-324094-23kzr8rq	222	20	human	human	JJ
cord-324094-23kzr8rq	222	21	papillomavirus	papillomavirus	NN
cord-324094-23kzr8rq	222	22	type	type	NN
cord-324094-23kzr8rq	222	23	6	6	CD
cord-324094-23kzr8rq	222	24	Development	development	NN
cord-324094-23kzr8rq	222	25	and	and	CC
cord-324094-23kzr8rq	222	26	evaluation	evaluation	NN
cord-324094-23kzr8rq	222	27	of	of	IN
cord-324094-23kzr8rq	222	28	a	a	DT
cord-324094-23kzr8rq	222	29	novel	novel	JJ
cord-324094-23kzr8rq	222	30	loop	loop	NN
cord-324094-23kzr8rq	222	31	-	-	HYPH
cord-324094-23kzr8rq	222	32	mediated	mediate	VBN
cord-324094-23kzr8rq	222	33	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	34	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	35	cation	cation	NN
cord-324094-23kzr8rq	222	36	method	method	NN
cord-324094-23kzr8rq	222	37	for	for	IN
cord-324094-23kzr8rq	222	38	rapid	rapid	JJ
cord-324094-23kzr8rq	222	39	detection	detection	NN
cord-324094-23kzr8rq	222	40	of	of	IN
cord-324094-23kzr8rq	222	41	severe	severe	JJ
cord-324094-23kzr8rq	222	42	acute	acute	JJ
cord-324094-23kzr8rq	222	43	respiratory	respiratory	JJ
cord-324094-23kzr8rq	222	44	syndrome	syndrome	NN
cord-324094-23kzr8rq	222	45	coronavirus	coronavirus	NN
cord-324094-23kzr8rq	222	46	Rapid	rapid	JJ
cord-324094-23kzr8rq	222	47	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	222	48	of	of	IN
cord-324094-23kzr8rq	222	49	H5N1	H5N1	NNP
cord-324094-23kzr8rq	222	50	avian	avian	JJ
cord-324094-23kzr8rq	222	51	infl	infl	NN
cord-324094-23kzr8rq	222	52	uenza	uenza	NNP
cord-324094-23kzr8rq	222	53	virus	virus	NN
cord-324094-23kzr8rq	222	54	infection	infection	NN
cord-324094-23kzr8rq	222	55	by	by	IN
cord-324094-23kzr8rq	222	56	newly	newly	RB
cord-324094-23kzr8rq	222	57	developed	develop	VBN
cord-324094-23kzr8rq	222	58	infl	infl	NN
cord-324094-23kzr8rq	222	59	uenza	uenza	NNP
cord-324094-23kzr8rq	222	60	H5	H5	NNP
cord-324094-23kzr8rq	222	61	hemagglutinin	hemagglutinin	NN
cord-324094-23kzr8rq	222	62	gene	gene	NN
cord-324094-23kzr8rq	222	63	-	-	HYPH
cord-324094-23kzr8rq	222	64	specifi	specifi	VBN
cord-324094-23kzr8rq	222	65	c	c	NN
cord-324094-23kzr8rq	222	66	loop	loop	NN
cord-324094-23kzr8rq	222	67	-	-	HYPH
cord-324094-23kzr8rq	222	68	mediated	mediate	VBN
cord-324094-23kzr8rq	222	69	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	70	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	71	cation	cation	NN
cord-324094-23kzr8rq	222	72	method	method	NN
cord-324094-23kzr8rq	222	73	Sensitive	sensitive	JJ
cord-324094-23kzr8rq	222	74	and	and	CC
cord-324094-23kzr8rq	222	75	quantitative	quantitative	JJ
cord-324094-23kzr8rq	222	76	detection	detection	NN
cord-324094-23kzr8rq	222	77	of	of	IN
cord-324094-23kzr8rq	222	78	severe	severe	JJ
cord-324094-23kzr8rq	222	79	acute	acute	JJ
cord-324094-23kzr8rq	222	80	respiratory	respiratory	JJ
cord-324094-23kzr8rq	222	81	syndrome	syndrome	NN
cord-324094-23kzr8rq	222	82	coronavirus	coronavirus	NN
cord-324094-23kzr8rq	222	83	infection	infection	NN
cord-324094-23kzr8rq	222	84	by	by	IN
cord-324094-23kzr8rq	222	85	real	real	JJ
cord-324094-23kzr8rq	222	86	-	-	HYPH
cord-324094-23kzr8rq	222	87	time	time	NN
cord-324094-23kzr8rq	222	88	nested	nested	JJ
cord-324094-23kzr8rq	222	89	polymerase	polymerase	NN
cord-324094-23kzr8rq	222	90	chain	chain	NN
cord-324094-23kzr8rq	222	91	reaction	reaction	NN
cord-324094-23kzr8rq	222	92	Sensitive	sensitive	JJ
cord-324094-23kzr8rq	222	93	and	and	CC
cord-324094-23kzr8rq	222	94	rapid	rapid	JJ
cord-324094-23kzr8rq	222	95	detection	detection	NN
cord-324094-23kzr8rq	222	96	of	of	IN
cord-324094-23kzr8rq	222	97	herpes	herpes	NNP
cord-324094-23kzr8rq	222	98	simplex	simplex	NNP
cord-324094-23kzr8rq	222	99	virus	virus	NN
cord-324094-23kzr8rq	222	100	and	and	CC
cord-324094-23kzr8rq	222	101	varicella	varicella	NN
cord-324094-23kzr8rq	222	102	-	-	HYPH
cord-324094-23kzr8rq	222	103	zoster	zoster	NN
cord-324094-23kzr8rq	222	104	virus	virus	NN
cord-324094-23kzr8rq	222	105	DNA	dna	NN
cord-324094-23kzr8rq	222	106	by	by	IN
cord-324094-23kzr8rq	222	107	loop	loop	NN
cord-324094-23kzr8rq	222	108	-	-	HYPH
cord-324094-23kzr8rq	222	109	mediated	mediate	VBN
cord-324094-23kzr8rq	222	110	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	111	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	112	cation	cation	NN
cord-324094-23kzr8rq	222	113	Real	real	JJ
cord-324094-23kzr8rq	222	114	-	-	HYPH
cord-324094-23kzr8rq	222	115	time	time	NN
cord-324094-23kzr8rq	222	116	PCR	PCR	NNP
cord-324094-23kzr8rq	222	117	in	in	IN
cord-324094-23kzr8rq	222	118	virology	virology	NN
cord-324094-23kzr8rq	222	119	Detection	detection	NN
cord-324094-23kzr8rq	222	120	of	of	IN
cord-324094-23kzr8rq	222	121	Loop	Loop	NNP
cord-324094-23kzr8rq	222	122	mediated	mediate	VBN
cord-324094-23kzr8rq	222	123	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	124	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	125	cation	cation	NN
cord-324094-23kzr8rq	222	126	reaction	reaction	NN
cord-324094-23kzr8rq	222	127	by	by	IN
cord-324094-23kzr8rq	222	128	turbidity	turbidity	NN
cord-324094-23kzr8rq	222	129	derived	derive	VBN
cord-324094-23kzr8rq	222	130	from	from	IN
cord-324094-23kzr8rq	222	131	magnesium	magnesium	NN
cord-324094-23kzr8rq	222	132	pyrophosphate	pyrophosphate	NN
cord-324094-23kzr8rq	222	133	formation	formation	NN
cord-324094-23kzr8rq	222	134	Real	real	JJ
cord-324094-23kzr8rq	222	135	-	-	HYPH
cord-324094-23kzr8rq	222	136	time	time	NN
cord-324094-23kzr8rq	222	137	turbidimetry	turbidimetry	NN
cord-324094-23kzr8rq	222	138	of	of	IN
cord-324094-23kzr8rq	222	139	LAMP	LAMP	NNP
cord-324094-23kzr8rq	222	140	reaction	reaction	NN
cord-324094-23kzr8rq	222	141	for	for	IN
cord-324094-23kzr8rq	222	142	quantifying	quantify	VBG
cord-324094-23kzr8rq	222	143	template	template	NN
cord-324094-23kzr8rq	222	144	DNA	dna	NN
cord-324094-23kzr8rq	222	145	Sequence	sequence	NN
cord-324094-23kzr8rq	222	146	specifi	specifi	NN
cord-324094-23kzr8rq	222	147	c	c	NN
cord-324094-23kzr8rq	222	148	visual	visual	JJ
cord-324094-23kzr8rq	222	149	detection	detection	NN
cord-324094-23kzr8rq	222	150	of	of	IN
cord-324094-23kzr8rq	222	151	LAMP	lamp	NN
cord-324094-23kzr8rq	222	152	reactions	reaction	NNS
cord-324094-23kzr8rq	222	153	by	by	IN
cord-324094-23kzr8rq	222	154	addition	addition	NN
cord-324094-23kzr8rq	222	155	of	of	IN
cord-324094-23kzr8rq	222	156	cationic	cationic	JJ
cord-324094-23kzr8rq	222	157	polymers	polymer	NNS
cord-324094-23kzr8rq	222	158	Accelerated	accelerate	VBD
cord-324094-23kzr8rq	222	159	reaction	reaction	NN
cord-324094-23kzr8rq	222	160	by	by	IN
cord-324094-23kzr8rq	222	161	loop	loop	NN
cord-324094-23kzr8rq	222	162	mediated	mediate	VBN
cord-324094-23kzr8rq	222	163	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	164	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	165	cation	cation	NN
cord-324094-23kzr8rq	222	166	using	use	VBG
cord-324094-23kzr8rq	222	167	loop	loop	NN
cord-324094-23kzr8rq	222	168	primers	primer	NNS
cord-324094-23kzr8rq	222	169	Loop	loop	NN
cord-324094-23kzr8rq	222	170	-	-	HYPH
cord-324094-23kzr8rq	222	171	mediated	mediate	VBN
cord-324094-23kzr8rq	222	172	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	173	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	174	cation	cation	NN
cord-324094-23kzr8rq	222	175	of	of	IN
cord-324094-23kzr8rq	222	176	DNA	DNA	NNP
cord-324094-23kzr8rq	222	177	Rapid	Rapid	NNP
cord-324094-23kzr8rq	222	178	detection	detection	NN
cord-324094-23kzr8rq	222	179	of	of	IN
cord-324094-23kzr8rq	222	180	Varicella	varicella	NN
cord-324094-23kzr8rq	222	181	-	-	HYPH
cord-324094-23kzr8rq	222	182	zoster	zoster	NN
cord-324094-23kzr8rq	222	183	virus	virus	NN
cord-324094-23kzr8rq	222	184	infection	infection	NN
cord-324094-23kzr8rq	222	185	by	by	IN
cord-324094-23kzr8rq	222	186	a	a	DT
cord-324094-23kzr8rq	222	187	loop	loop	NN
cord-324094-23kzr8rq	222	188	-	-	HYPH
cord-324094-23kzr8rq	222	189	mediated	mediate	VBN
cord-324094-23kzr8rq	222	190	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	191	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	192	cation	cation	NN
cord-324094-23kzr8rq	222	193	method	method	NN
cord-324094-23kzr8rq	222	194	Real	Real	NNP
cord-324094-23kzr8rq	222	195	-	-	HYPH
cord-324094-23kzr8rq	222	196	Time	time	NN
cord-324094-23kzr8rq	222	197	Reverse	Reverse	NNP
cord-324094-23kzr8rq	222	198	Transcription	Transcription	NNP
cord-324094-23kzr8rq	222	199	Loop	Loop	NNP
cord-324094-23kzr8rq	222	200	mediated	mediate	VBN
cord-324094-23kzr8rq	222	201	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	202	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	203	cation	cation	NN
cord-324094-23kzr8rq	222	204	for	for	IN
cord-324094-23kzr8rq	222	205	rapid	rapid	JJ
cord-324094-23kzr8rq	222	206	detection	detection	NN
cord-324094-23kzr8rq	222	207	of	of	IN
cord-324094-23kzr8rq	222	208	West	West	NNP
cord-324094-23kzr8rq	222	209	Nile	Nile	NNP
cord-324094-23kzr8rq	222	210	Virus	Virus	NNP
cord-324094-23kzr8rq	222	211	Rapid	Rapid	NNP
cord-324094-23kzr8rq	222	212	detection	detection	NN
cord-324094-23kzr8rq	222	213	and	and	CC
cord-324094-23kzr8rq	222	214	differentiation	differentiation	NN
cord-324094-23kzr8rq	222	215	of	of	IN
cord-324094-23kzr8rq	222	216	dengue	dengue	NN
cord-324094-23kzr8rq	222	217	virus	virus	NN
cord-324094-23kzr8rq	222	218	serotypes	serotype	NNS
cord-324094-23kzr8rq	222	219	by	by	IN
cord-324094-23kzr8rq	222	220	a	a	DT
cord-324094-23kzr8rq	222	221	real	real	JJ
cord-324094-23kzr8rq	222	222	-	-	HYPH
cord-324094-23kzr8rq	222	223	time	time	NN
cord-324094-23kzr8rq	222	224	reverse	reverse	JJ
cord-324094-23kzr8rq	222	225	transcription	transcription	NN
cord-324094-23kzr8rq	222	226	-	-	HYPH
cord-324094-23kzr8rq	222	227	loop	loop	NN
cord-324094-23kzr8rq	222	228	-	-	HYPH
cord-324094-23kzr8rq	222	229	mediated	mediate	VBN
cord-324094-23kzr8rq	222	230	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	231	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	232	cation	cation	NN
cord-324094-23kzr8rq	222	233	assay	assay	NN
cord-324094-23kzr8rq	222	234	Development	development	NN
cord-324094-23kzr8rq	222	235	and	and	CC
cord-324094-23kzr8rq	222	236	evaluation	evaluation	NN
cord-324094-23kzr8rq	222	237	of	of	IN
cord-324094-23kzr8rq	222	238	reverse	reverse	JJ
cord-324094-23kzr8rq	222	239	transcription	transcription	NN
cord-324094-23kzr8rq	222	240	Loop	loop	NN
cord-324094-23kzr8rq	222	241	mediated	mediate	VBD
cord-324094-23kzr8rq	222	242	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	243	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	244	cation	cation	NN
cord-324094-23kzr8rq	222	245	assay	assay	NN
cord-324094-23kzr8rq	222	246	for	for	IN
cord-324094-23kzr8rq	222	247	rapid	rapid	JJ
cord-324094-23kzr8rq	222	248	and	and	CC
cord-324094-23kzr8rq	222	249	Real	real	JJ
cord-324094-23kzr8rq	222	250	-	-	HYPH
cord-324094-23kzr8rq	222	251	time	time	NN
cord-324094-23kzr8rq	222	252	detection	detection	NN
cord-324094-23kzr8rq	222	253	of	of	IN
cord-324094-23kzr8rq	222	254	Japanese	japanese	JJ
cord-324094-23kzr8rq	222	255	encephalitis	encephalitis	RB
cord-324094-23kzr8rq	222	256	virus	virus	NN
cord-324094-23kzr8rq	222	257	Rapid	Rapid	NNP
cord-324094-23kzr8rq	222	258	and	and	CC
cord-324094-23kzr8rq	222	259	realtime	realtime	JJ
cord-324094-23kzr8rq	222	260	detection	detection	NN
cord-324094-23kzr8rq	222	261	of	of	IN
cord-324094-23kzr8rq	222	262	chikungunya	chikungunya	NN
cord-324094-23kzr8rq	222	263	virus	virus	NN
cord-324094-23kzr8rq	222	264	by	by	IN
cord-324094-23kzr8rq	222	265	reverse	reverse	JJ
cord-324094-23kzr8rq	222	266	transcription	transcription	NN
cord-324094-23kzr8rq	222	267	loop	loop	NN
cord-324094-23kzr8rq	222	268	mediated	mediate	VBN
cord-324094-23kzr8rq	222	269	isothermal	isothermal	JJ
cord-324094-23kzr8rq	222	270	amplifi	amplifi	NNP
cord-324094-23kzr8rq	222	271	cation	cation	NN
cord-324094-23kzr8rq	222	272	assay	assay	NN
cord-324094-23kzr8rq	222	273	Rapid	Rapid	NNP
cord-324094-23kzr8rq	222	274	and	and	CC
cord-324094-23kzr8rq	222	275	Real	real	JJ
cord-324094-23kzr8rq	222	276	-	-	HYPH
cord-324094-23kzr8rq	222	277	time	time	NN
cord-324094-23kzr8rq	222	278	Assays	Assays	NNPS
cord-324094-23kzr8rq	222	279	for	for	IN
cord-324094-23kzr8rq	222	280	Detection	Detection	NNP
cord-324094-23kzr8rq	222	281	and	and	CC
cord-324094-23kzr8rq	222	282	Quantifi	Quantifi	NNP
cord-324094-23kzr8rq	222	283	cation	cation	NN
cord-324094-23kzr8rq	222	284	of	of	IN
cord-324094-23kzr8rq	222	285	Chikungunya	Chikungunya	NNP
cord-324094-23kzr8rq	222	286	Virus	Virus	NNP
cord-324094-23kzr8rq	222	287	Early	early	JJ
cord-324094-23kzr8rq	222	288	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	222	289	of	of	IN
cord-324094-23kzr8rq	222	290	SARS	SARS	NNP
cord-324094-23kzr8rq	222	291	coronavirus	coronavirus	NN
cord-324094-23kzr8rq	222	292	infection	infection	NN
cord-324094-23kzr8rq	222	293	by	by	IN
cord-324094-23kzr8rq	222	294	real	real	JJ
cord-324094-23kzr8rq	222	295	time	time	NN
cord-324094-23kzr8rq	223	1	RT	RT	NNP
cord-324094-23kzr8rq	223	2	-	-	HYPH
cord-324094-23kzr8rq	223	3	PCR	PCR	NNP
cord-324094-23kzr8rq	223	4	Molecular	molecular	JJ
cord-324094-23kzr8rq	223	5	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	223	6	of	of	IN
cord-324094-23kzr8rq	223	7	medical	medical	JJ
cord-324094-23kzr8rq	223	8	viruses	virus	NNS
cord-324094-23kzr8rq	224	1	Quantitative	quantitative	JJ
cord-324094-23kzr8rq	224	2	real	real	JJ
cord-324094-23kzr8rq	224	3	-	-	HYPH
cord-324094-23kzr8rq	224	4	time	time	NN
cord-324094-23kzr8rq	224	5	PCR	PCR	NNP
cord-324094-23kzr8rq	224	6	on	on	IN
cord-324094-23kzr8rq	224	7	Lightcycler	Lightcycler	NNP
cord-324094-23kzr8rq	224	8	for	for	IN
cord-324094-23kzr8rq	224	9	the	the	DT
cord-324094-23kzr8rq	224	10	detection	detection	NN
cord-324094-23kzr8rq	224	11	of	of	IN
cord-324094-23kzr8rq	224	12	human	human	JJ
cord-324094-23kzr8rq	224	13	immunodefi	immunodefi	NNP
cord-324094-23kzr8rq	224	14	ciency	ciency	NNP
cord-324094-23kzr8rq	224	15	virus	virus	NN
cord-324094-23kzr8rq	224	16	type	type	NN
cord-324094-23kzr8rq	224	17	Development	Development	NNP
cord-324094-23kzr8rq	224	18	and	and	CC
cord-324094-23kzr8rq	224	19	Evaluation	Evaluation	NNP
cord-324094-23kzr8rq	224	20	of	of	IN
cord-324094-23kzr8rq	224	21	SYBR	SYBR	NNP
cord-324094-23kzr8rq	224	22	Green	Green	NNP
cord-324094-23kzr8rq	225	1	I	-PRON-	PRP
cord-324094-23kzr8rq	225	2	based	base	VBD
cord-324094-23kzr8rq	225	3	One	one	CD
cord-324094-23kzr8rq	225	4	Step	Step	NNP
cord-324094-23kzr8rq	225	5	Real	real	JJ
cord-324094-23kzr8rq	225	6	time	time	NN
cord-324094-23kzr8rq	226	1	RT	RT	NNP
cord-324094-23kzr8rq	226	2	-	-	HYPH
cord-324094-23kzr8rq	226	3	PCR	PCR	NNP
cord-324094-23kzr8rq	226	4	assay	assay	NN
cord-324094-23kzr8rq	226	5	for	for	IN
cord-324094-23kzr8rq	226	6	detection	detection	NN
cord-324094-23kzr8rq	226	7	and	and	CC
cord-324094-23kzr8rq	226	8	quantifi	quantifi	NN
cord-324094-23kzr8rq	226	9	cation	cation	NN
cord-324094-23kzr8rq	226	10	of	of	IN
cord-324094-23kzr8rq	226	11	Chikungunya	Chikungunya	NNP
cord-324094-23kzr8rq	226	12	virus	virus	NN
cord-324094-23kzr8rq	226	13	Development	Development	NNP
cord-324094-23kzr8rq	226	14	and	and	CC
cord-324094-23kzr8rq	226	15	Evaluation	Evaluation	NNP
cord-324094-23kzr8rq	226	16	of	of	IN
cord-324094-23kzr8rq	226	17	SYBR	SYBR	NNP
cord-324094-23kzr8rq	226	18	Green	Green	NNP
cord-324094-23kzr8rq	227	1	I	-PRON-	PRP
cord-324094-23kzr8rq	227	2	based	base	VBD
cord-324094-23kzr8rq	227	3	One	one	CD
cord-324094-23kzr8rq	227	4	Step	Step	NNP
cord-324094-23kzr8rq	227	5	Real	real	JJ
cord-324094-23kzr8rq	227	6	time	time	NN
cord-324094-23kzr8rq	228	1	RT	RT	NNP
cord-324094-23kzr8rq	228	2	-	-	HYPH
cord-324094-23kzr8rq	228	3	PCR	PCR	NNP
cord-324094-23kzr8rq	228	4	assay	assay	NN
cord-324094-23kzr8rq	228	5	for	for	IN
cord-324094-23kzr8rq	228	6	detection	detection	NN
cord-324094-23kzr8rq	228	7	and	and	CC
cord-324094-23kzr8rq	228	8	quantifi	quantifi	NN
cord-324094-23kzr8rq	228	9	cation	cation	NN
cord-324094-23kzr8rq	228	10	of	of	IN
cord-324094-23kzr8rq	228	11	Japanese	japanese	JJ
cord-324094-23kzr8rq	228	12	encephalitis	encephalitis	RB
cord-324094-23kzr8rq	228	13	virus	virus	NN
cord-324094-23kzr8rq	228	14	Fast	fast	JJ
cord-324094-23kzr8rq	228	15	detection	detection	NN
cord-324094-23kzr8rq	228	16	of	of	IN
cord-324094-23kzr8rq	228	17	Noroviruses	Noroviruses	NNPS
cord-324094-23kzr8rq	228	18	using	use	VBG
cord-324094-23kzr8rq	228	19	a	a	DT
cord-324094-23kzr8rq	228	20	real	real	JJ
cord-324094-23kzr8rq	228	21	-	-	HYPH
cord-324094-23kzr8rq	228	22	time	time	NN
cord-324094-23kzr8rq	228	23	PCR	pcr	NN
cord-324094-23kzr8rq	228	24	assay	assay	NN
cord-324094-23kzr8rq	228	25	and	and	CC
cord-324094-23kzr8rq	228	26	automated	automate	VBN
cord-324094-23kzr8rq	228	27	sample	sample	NN
cord-324094-23kzr8rq	228	28	preparation	preparation	NN
cord-324094-23kzr8rq	228	29	Detection	detection	NN
cord-324094-23kzr8rq	228	30	and	and	CC
cord-324094-23kzr8rq	228	31	quantitation	quantitation	NN
cord-324094-23kzr8rq	228	32	of	of	IN
cord-324094-23kzr8rq	228	33	group	group	NN
cord-324094-23kzr8rq	229	1	A	a	DT
cord-324094-23kzr8rq	229	2	rotaviruses	rotavirus	NNS
cord-324094-23kzr8rq	229	3	by	by	IN
cord-324094-23kzr8rq	229	4	competitive	competitive	JJ
cord-324094-23kzr8rq	229	5	and	and	CC
cord-324094-23kzr8rq	229	6	real	real	JJ
cord-324094-23kzr8rq	229	7	-	-	HYPH
cord-324094-23kzr8rq	229	8	time	time	NN
cord-324094-23kzr8rq	229	9	reverse	reverse	NN
cord-324094-23kzr8rq	229	10	transcription	transcription	NN
cord-324094-23kzr8rq	229	11	-	-	HYPH
cord-324094-23kzr8rq	229	12	polymerase	polymerase	NN
cord-324094-23kzr8rq	229	13	chain	chain	NN
cord-324094-23kzr8rq	229	14	reaction	reaction	NN
cord-324094-23kzr8rq	229	15	Development	Development	NNP
cord-324094-23kzr8rq	229	16	of	of	IN
cord-324094-23kzr8rq	229	17	group	group	NN
cord-324094-23kzr8rq	229	18	-	-	HYPH
cord-324094-23kzr8rq	229	19	and	and	CC
cord-324094-23kzr8rq	229	20	serotypespecifi	serotypespecifi	NNP
cord-324094-23kzr8rq	229	21	c	c	NNP
cord-324094-23kzr8rq	229	22	one	one	CD
cord-324094-23kzr8rq	229	23	-	-	HYPH
cord-324094-23kzr8rq	229	24	step	step	NN
cord-324094-23kzr8rq	229	25	SYBR	SYBR	NNP
cord-324094-23kzr8rq	229	26	green	green	JJ
cord-324094-23kzr8rq	229	27	I	I	NNP
cord-324094-23kzr8rq	229	28	-	-	HYPH
cord-324094-23kzr8rq	229	29	based	base	VBN
cord-324094-23kzr8rq	229	30	real	real	JJ
cord-324094-23kzr8rq	229	31	-	-	HYPH
cord-324094-23kzr8rq	229	32	time	time	NN
cord-324094-23kzr8rq	229	33	reverse	reverse	NN
cord-324094-23kzr8rq	229	34	transcription	transcription	NN
cord-324094-23kzr8rq	229	35	-	-	HYPH
cord-324094-23kzr8rq	229	36	PCR	pcr	NN
cord-324094-23kzr8rq	229	37	assay	assay	NN
cord-324094-23kzr8rq	229	38	for	for	IN
cord-324094-23kzr8rq	229	39	dengue	dengue	NN
cord-324094-23kzr8rq	229	40	virus	virus	NN
cord-324094-23kzr8rq	229	41	Comparison	comparison	NN
cord-324094-23kzr8rq	229	42	of	of	IN
cord-324094-23kzr8rq	229	43	loop	loop	NN
cord-324094-23kzr8rq	229	44	-	-	HYPH
cord-324094-23kzr8rq	229	45	mediated	mediate	VBN
cord-324094-23kzr8rq	229	46	isothermal	isothermal	JJ
cord-324094-23kzr8rq	229	47	amplifi	amplifi	NNP
cord-324094-23kzr8rq	229	48	cation	cation	NN
cord-324094-23kzr8rq	229	49	,	,	,
cord-324094-23kzr8rq	229	50	real	real	JJ
cord-324094-23kzr8rq	229	51	-	-	HYPH
cord-324094-23kzr8rq	229	52	time	time	NN
cord-324094-23kzr8rq	229	53	PCR	PCR	NNP
cord-324094-23kzr8rq	229	54	,	,	,
cord-324094-23kzr8rq	229	55	and	and	CC
cord-324094-23kzr8rq	229	56	virus	virus	NN
cord-324094-23kzr8rq	229	57	isolation	isolation	NN
cord-324094-23kzr8rq	229	58	for	for	IN
cord-324094-23kzr8rq	229	59	the	the	DT
cord-324094-23kzr8rq	229	60	detection	detection	NN
cord-324094-23kzr8rq	229	61	of	of	IN
cord-324094-23kzr8rq	229	62	herpes	herpes	NNP
cord-324094-23kzr8rq	229	63	simplex	simplex	NN
cord-324094-23kzr8rq	229	64	virus	virus	NN
cord-324094-23kzr8rq	229	65	in	in	IN
cord-324094-23kzr8rq	229	66	genital	genital	JJ
cord-324094-23kzr8rq	229	67	lesions	lesion	NNS
cord-324094-23kzr8rq	229	68	Development	Development	NNP
cord-324094-23kzr8rq	229	69	of	of	IN
cord-324094-23kzr8rq	229	70	the	the	DT
cord-324094-23kzr8rq	229	71	loop	loop	NN
cord-324094-23kzr8rq	229	72	-	-	HYPH
cord-324094-23kzr8rq	229	73	mediated	mediate	VBN
cord-324094-23kzr8rq	229	74	isothermal	isothermal	JJ
cord-324094-23kzr8rq	229	75	amplifi	amplifi	NNP
cord-324094-23kzr8rq	229	76	cation	cation	NN
cord-324094-23kzr8rq	229	77	method	method	NN
cord-324094-23kzr8rq	229	78	for	for	IN
cord-324094-23kzr8rq	229	79	rapid	rapid	JJ
cord-324094-23kzr8rq	229	80	detection	detection	NN
cord-324094-23kzr8rq	229	81	of	of	IN
cord-324094-23kzr8rq	229	82	cytomegalovirus	cytomegalovirus	NNP
cord-324094-23kzr8rq	229	83	DNA	DNA	NNP
cord-324094-23kzr8rq	229	84	Rapid	Rapid	NNP
cord-324094-23kzr8rq	229	85	and	and	CC
cord-324094-23kzr8rq	229	86	sensitive	sensitive	JJ
cord-324094-23kzr8rq	229	87	method	method	NN
cord-324094-23kzr8rq	229	88	using	use	VBG
cord-324094-23kzr8rq	229	89	multiplex	multiplex	JJ
cord-324094-23kzr8rq	229	90	real	real	JJ
cord-324094-23kzr8rq	229	91	-	-	HYPH
cord-324094-23kzr8rq	229	92	time	time	NN
cord-324094-23kzr8rq	229	93	PCR	pcr	NN
cord-324094-23kzr8rq	229	94	for	for	IN
cord-324094-23kzr8rq	229	95	diagnosis	diagnosis	NN
cord-324094-23kzr8rq	229	96	of	of	IN
cord-324094-23kzr8rq	229	97	infections	infection	NNS
cord-324094-23kzr8rq	229	98	by	by	IN
cord-324094-23kzr8rq	229	99	infl	infl	NNP
cord-324094-23kzr8rq	229	100	uenza	uenza	NNP
cord-324094-23kzr8rq	229	101	a	a	NNP
cord-324094-23kzr8rq	229	102	and	and	CC
cord-324094-23kzr8rq	229	103	infl	infl	NN
cord-324094-23kzr8rq	229	104	uenza	uenza	NN
cord-324094-23kzr8rq	229	105	B	b	NN
cord-324094-23kzr8rq	229	106	viruses	virus	NNS
cord-324094-23kzr8rq	229	107	,	,	,
cord-324094-23kzr8rq	229	108	respiratory	respiratory	JJ
cord-324094-23kzr8rq	229	109	syncytial	syncytial	JJ
cord-324094-23kzr8rq	229	110	virus	virus	NN
cord-324094-23kzr8rq	229	111	Rapid	Rapid	NNP
cord-324094-23kzr8rq	229	112	detection	detection	NN
cord-324094-23kzr8rq	229	113	and	and	CC
cord-324094-23kzr8rq	229	114	quantifi	quantifi	NN
cord-324094-23kzr8rq	229	115	cation	cation	NN
cord-324094-23kzr8rq	229	116	of	of	IN
cord-324094-23kzr8rq	229	117	Japanese	japanese	JJ
cord-324094-23kzr8rq	229	118	encephalitis	encephalitis	RB
cord-324094-23kzr8rq	229	119	virus	virus	NN
cord-324094-23kzr8rq	229	120	by	by	IN
cord-324094-23kzr8rq	229	121	real	real	JJ
cord-324094-23kzr8rq	229	122	-	-	HYPH
cord-324094-23kzr8rq	229	123	time	time	NN
cord-324094-23kzr8rq	229	124	reverse	reverse	NN
cord-324094-23kzr8rq	229	125	transcription	transcription	NN
cord-324094-23kzr8rq	229	126	loop	loop	NN
cord-324094-23kzr8rq	229	127	-	-	HYPH
cord-324094-23kzr8rq	229	128	mediated	mediate	VBN
cord-324094-23kzr8rq	229	129	isothermal	isothermal	JJ
cord-324094-23kzr8rq	229	130	amplifi	amplifi	NNP
cord-324094-23kzr8rq	229	131	cation	cation	NN
cord-324094-23kzr8rq	229	132	Rapid	Rapid	NNP
cord-324094-23kzr8rq	229	133	and	and	CC
cord-324094-23kzr8rq	229	134	sensitive	sensitive	JJ
cord-324094-23kzr8rq	229	135	detection	detection	NN
cord-324094-23kzr8rq	229	136	of	of	IN
cord-324094-23kzr8rq	229	137	mumps	mumps	NN
cord-324094-23kzr8rq	229	138	virus	virus	NN
cord-324094-23kzr8rq	229	139	RNA	RNA	NNP
cord-324094-23kzr8rq	229	140	directly	directly	RB
cord-324094-23kzr8rq	229	141	from	from	IN
cord-324094-23kzr8rq	229	142	clinical	clinical	JJ
cord-324094-23kzr8rq	229	143	samples	sample	NNS
cord-324094-23kzr8rq	229	144	by	by	IN
cord-324094-23kzr8rq	229	145	real	real	JJ
cord-324094-23kzr8rq	229	146	-	-	HYPH
cord-324094-23kzr8rq	229	147	time	time	NN
cord-324094-23kzr8rq	229	148	PCR	PCR	NNP
cord-324094-23kzr8rq	229	149	Principle	Principle	NNP
cord-324094-23kzr8rq	229	150	of	of	IN
cord-324094-23kzr8rq	229	151	LAMP	LAMP	NNP
cord-324094-23kzr8rq	229	152	method	method	NN
cord-324094-23kzr8rq	229	153	-a	-a	:
cord-324094-23kzr8rq	229	154	simple	simple	JJ
cord-324094-23kzr8rq	229	155	and	and	CC
cord-324094-23kzr8rq	229	156	rapid	rapid	JJ
cord-324094-23kzr8rq	229	157	gene	gene	NN
cord-324094-23kzr8rq	229	158	amplifi	amplifi	NNP
cord-324094-23kzr8rq	229	159	cation	cation	NN
cord-324094-23kzr8rq	229	160	method	method	NN
cord-324094-23kzr8rq	229	161	Rapid	Rapid	NNP
cord-324094-23kzr8rq	229	162	detection	detection	NN
cord-324094-23kzr8rq	229	163	of	of	IN
cord-324094-23kzr8rq	229	164	enterovirus	enterovirus	NN
cord-324094-23kzr8rq	229	165	RNA	RNA	NNP
cord-324094-23kzr8rq	229	166	in	in	IN
cord-324094-23kzr8rq	229	167	cerebrospinal	cerebrospinal	JJ
cord-324094-23kzr8rq	229	168	fl	fl	NNP
cord-324094-23kzr8rq	229	169	uid	uid	NN
cord-324094-23kzr8rq	229	170	specimens	specimen	NNS
cord-324094-23kzr8rq	229	171	with	with	IN
cord-324094-23kzr8rq	229	172	a	a	DT
cord-324094-23kzr8rq	229	173	novel	novel	JJ
cord-324094-23kzr8rq	229	174	single	single	JJ
cord-324094-23kzr8rq	229	175	-	-	HYPH
cord-324094-23kzr8rq	229	176	tube	tube	NN
cord-324094-23kzr8rq	229	177	realtime	realtime	NN
cord-324094-23kzr8rq	229	178	reverse	reverse	NN
cord-324094-23kzr8rq	229	179	transcription	transcription	NN
cord-324094-23kzr8rq	229	180	-	-	HYPH
cord-324094-23kzr8rq	229	181	PCR	pcr	NN
cord-324094-23kzr8rq	229	182	assay	assay	NN
cord-324094-23kzr8rq	229	183	Detection	detection	NN
cord-324094-23kzr8rq	229	184	and	and	CC
cord-324094-23kzr8rq	229	185	monitoring	monitoring	NN
cord-324094-23kzr8rq	229	186	of	of	IN
cord-324094-23kzr8rq	229	187	virus	virus	NN
cord-324094-23kzr8rq	229	188	infections	infection	NNS
cord-324094-23kzr8rq	229	189	by	by	IN
cord-324094-23kzr8rq	229	190	real	real	JJ
cord-324094-23kzr8rq	229	191	-	-	HYPH
cord-324094-23kzr8rq	229	192	time	time	NN
cord-324094-23kzr8rq	229	193	PCR	pcr	NN