id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-016938-pk76snuy	Suh, Hwal (Matthew)	Collagen Fabrication for the Cell-based Implants in Regenerative Medicine	2008		.txt	text/plain	6409	292	37	As atelocollagen has no telopeptides that integrate with other atelocollagen molecules, basic technology to produce intramolecular and intermolecular bonds for construction of the extracellular matrix with adequate mechanical properties required by tissue where the artificial cell-based implant is delivered is the recrosslinking method using chemical reagents or physical dynamics. Collagen can be fabricated into various forms of gel, fiber, membrane with or without pores, and it can even be grafted onto the non-viable metal, ceramic and synthetic biomaterials to introduce biological layer on surface. To produce an artificial skin, autologous dermal fibroblasts of rat were seeded into the EDC crosslinked porous collagen-laminin membrane and cultured in minimum essential medium (MEM) for 3 days to provide the cell-niche adaptation period. Although collagen based gel is favorable to fabricate cell conductive substitute, weak mechanical property is a barrier for application in the physiological stress bearing tissues, and, to resolve this problem, hybridization of collagen with polymeric biomaterials has been suggested.	./cache/cord-016938-pk76snuy.txt	./txt/cord-016938-pk76snuy.txt