id	sid	tid	token	lemma	pos
cord-104279-choywmwd	1	1	key	key	NN
cord-104279-choywmwd	1	2	:	:	:
cord-104279-choywmwd	1	3	cord-104279-choywmwd	cord-104279-choywmwd	NNP
cord-104279-choywmwd	2	1	authors	author	NNS
cord-104279-choywmwd	2	2	:	:	:
cord-104279-choywmwd	3	1	nan	nan	NNP
cord-104279-choywmwd	3	2	title	title	NN
cord-104279-choywmwd	3	3	:	:	:
cord-104279-choywmwd	3	4	Membrane	membrane	NN
cord-104279-choywmwd	3	5	protein	protein	NN
cord-104279-choywmwd	3	6	sorting	sort	VBG
cord-104279-choywmwd	3	7	in	in	IN
cord-104279-choywmwd	3	8	the	the	DT
cord-104279-choywmwd	3	9	yeast	yeast	NN
cord-104279-choywmwd	3	10	secretory	secretory	NN
cord-104279-choywmwd	3	11	pathway	pathway	NN
cord-104279-choywmwd	3	12	:	:	:
cord-104279-choywmwd	3	13	evidence	evidence	NN
cord-104279-choywmwd	3	14	that	that	IN
cord-104279-choywmwd	3	15	the	the	DT
cord-104279-choywmwd	3	16	vacuole	vacuole	NN
cord-104279-choywmwd	3	17	may	may	MD
cord-104279-choywmwd	3	18	be	be	VB
cord-104279-choywmwd	3	19	the	the	DT
cord-104279-choywmwd	3	20	default	default	NN
cord-104279-choywmwd	3	21	compartment	compartment	NN
cord-104279-choywmwd	3	22	date	date	NN
cord-104279-choywmwd	3	23	:	:	:
cord-104279-choywmwd	3	24	1992	1992	CD
cord-104279-choywmwd	3	25	-	-	SYM
cord-104279-choywmwd	3	26	10	10	CD
cord-104279-choywmwd	3	27	-	-	HYPH
cord-104279-choywmwd	3	28	01	01	CD
cord-104279-choywmwd	3	29	journal	journal	NN
cord-104279-choywmwd	3	30	:	:	:
cord-104279-choywmwd	3	31	J	J	NNP
cord-104279-choywmwd	3	32	Cell	Cell	NNP
cord-104279-choywmwd	3	33	Biol	Biol	NNP
cord-104279-choywmwd	3	34	DOI	DOI	NNP
cord-104279-choywmwd	3	35	:	:	:
cord-104279-choywmwd	3	36	nan	nan	NNP
cord-104279-choywmwd	3	37	sha	sha	NNP
cord-104279-choywmwd	3	38	:	:	:
cord-104279-choywmwd	3	39	8894efb9895e24c82697cf864b69e9a2eb0751c2	8894efb9895e24c82697cf864b69e9a2eb0751c2	CD
cord-104279-choywmwd	3	40	doc_id	doc_id	CD
cord-104279-choywmwd	3	41	:	:	:
cord-104279-choywmwd	3	42	104279	104279	CD
cord-104279-choywmwd	3	43	cord_uid	cord_uid	NNS
cord-104279-choywmwd	3	44	:	:	:
cord-104279-choywmwd	4	1	choywmwd	choywmwd	NNP
cord-104279-choywmwd	5	1	The	the	DT
cord-104279-choywmwd	5	2	targeting	target	VBG
cord-104279-choywmwd	5	3	signals	signal	NNS
cord-104279-choywmwd	5	4	of	of	IN
cord-104279-choywmwd	5	5	two	two	CD
cord-104279-choywmwd	5	6	yeast	yeast	NN
cord-104279-choywmwd	5	7	integral	integral	JJ
cord-104279-choywmwd	5	8	membrane	membrane	NN
cord-104279-choywmwd	5	9	dipeptidyl	dipeptidyl	NNP
cord-104279-choywmwd	5	10	aminopeptidases	aminopeptidases	NNPS
cord-104279-choywmwd	5	11	(	(	-LRB-
cord-104279-choywmwd	5	12	DPAPs	DPAPs	NNP
cord-104279-choywmwd	5	13	)	)	-RRB-
cord-104279-choywmwd	5	14	,	,	,
cord-104279-choywmwd	5	15	DPAP	DPAP	NNP
cord-104279-choywmwd	5	16	B	B	NNP
cord-104279-choywmwd	5	17	and	and	CC
cord-104279-choywmwd	5	18	DPAP	DPAP	NNP
cord-104279-choywmwd	5	19	A	A	NNP
cord-104279-choywmwd	5	20	,	,	,
cord-104279-choywmwd	5	21	which	which	WDT
cord-104279-choywmwd	5	22	reside	reside	VBP
cord-104279-choywmwd	5	23	in	in	IN
cord-104279-choywmwd	5	24	the	the	DT
cord-104279-choywmwd	5	25	vacuole	vacuole	NN
cord-104279-choywmwd	5	26	and	and	CC
cord-104279-choywmwd	5	27	the	the	DT
cord-104279-choywmwd	5	28	Golgi	Golgi	NNP
cord-104279-choywmwd	5	29	apparatus	apparatus	NN
cord-104279-choywmwd	5	30	,	,	,
cord-104279-choywmwd	5	31	respectively	respectively	RB
cord-104279-choywmwd	5	32	,	,	,
cord-104279-choywmwd	5	33	were	be	VBD
cord-104279-choywmwd	5	34	analyzed	analyze	VBN
cord-104279-choywmwd	5	35	.	.	.
cord-104279-choywmwd	6	1	No	no	DT
cord-104279-choywmwd	6	2	single	single	JJ
cord-104279-choywmwd	6	3	domain	domain	NN
cord-104279-choywmwd	6	4	of	of	IN
cord-104279-choywmwd	6	5	DPAP	DPAP	NNP
cord-104279-choywmwd	6	6	B	B	NNP
cord-104279-choywmwd	6	7	is	be	VBZ
cord-104279-choywmwd	6	8	required	require	VBN
cord-104279-choywmwd	6	9	for	for	IN
cord-104279-choywmwd	6	10	delivery	delivery	NN
cord-104279-choywmwd	6	11	to	to	IN
cord-104279-choywmwd	6	12	the	the	DT
cord-104279-choywmwd	6	13	vacuolar	vacuolar	NNP
cord-104279-choywmwd	6	14	membrane	membrane	NN
cord-104279-choywmwd	6	15	,	,	,
cord-104279-choywmwd	6	16	because	because	IN
cord-104279-choywmwd	6	17	removal	removal	NN
cord-104279-choywmwd	6	18	or	or	CC
cord-104279-choywmwd	6	19	replacement	replacement	NN
cord-104279-choywmwd	6	20	of	of	IN
cord-104279-choywmwd	6	21	either	either	CC
cord-104279-choywmwd	6	22	the	the	DT
cord-104279-choywmwd	6	23	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	6	24	,	,	,
cord-104279-choywmwd	6	25	transmembrane	transmembrane	NN
cord-104279-choywmwd	6	26	,	,	,
cord-104279-choywmwd	6	27	or	or	CC
cord-104279-choywmwd	6	28	lumenal	lumenal	JJ
cord-104279-choywmwd	6	29	domain	domain	NN
cord-104279-choywmwd	6	30	did	do	VBD
cord-104279-choywmwd	6	31	not	not	RB
cord-104279-choywmwd	6	32	affect	affect	VB
cord-104279-choywmwd	6	33	the	the	DT
cord-104279-choywmwd	6	34	protein	protein	NN
cord-104279-choywmwd	6	35	's	's	POS
cord-104279-choywmwd	6	36	transport	transport	NN
cord-104279-choywmwd	6	37	to	to	IN
cord-104279-choywmwd	6	38	the	the	DT
cord-104279-choywmwd	6	39	vacuole	vacuole	NN
cord-104279-choywmwd	6	40	.	.	.
cord-104279-choywmwd	7	1	DPAP	DPAP	NNP
cord-104279-choywmwd	7	2	A	A	NNP
cord-104279-choywmwd	7	3	was	be	VBD
cord-104279-choywmwd	7	4	localized	localize	VBN
cord-104279-choywmwd	7	5	by	by	IN
cord-104279-choywmwd	7	6	indirect	indirect	JJ
cord-104279-choywmwd	7	7	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	7	8	to	to	IN
cord-104279-choywmwd	7	9	non	non	JJ
cord-104279-choywmwd	7	10	-	-	JJ
cord-104279-choywmwd	7	11	vacuolar	vacuolar	JJ
cord-104279-choywmwd	7	12	,	,	,
cord-104279-choywmwd	7	13	punctate	punctate	NN
cord-104279-choywmwd	7	14	structures	structure	NNS
cord-104279-choywmwd	7	15	characteristic	characteristic	JJ
cord-104279-choywmwd	7	16	of	of	IN
cord-104279-choywmwd	7	17	the	the	DT
cord-104279-choywmwd	7	18	yeast	yeast	NN
cord-104279-choywmwd	7	19	Golgi	Golgi	NNP
cord-104279-choywmwd	7	20	apparatus	apparatus	NN
cord-104279-choywmwd	7	21	.	.	.
cord-104279-choywmwd	8	1	The	the	DT
cord-104279-choywmwd	8	2	118-amino	118-amino	CD
cord-104279-choywmwd	8	3	acid	acid	NN
cord-104279-choywmwd	8	4	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	8	5	domain	domain	NN
cord-104279-choywmwd	8	6	of	of	IN
cord-104279-choywmwd	8	7	DPAP	DPAP	NNP
cord-104279-choywmwd	8	8	A	A	NNP
cord-104279-choywmwd	8	9	is	be	VBZ
cord-104279-choywmwd	8	10	sufficient	sufficient	JJ
cord-104279-choywmwd	8	11	for	for	IN
cord-104279-choywmwd	8	12	retention	retention	NN
cord-104279-choywmwd	8	13	of	of	IN
cord-104279-choywmwd	8	14	the	the	DT
cord-104279-choywmwd	8	15	protein	protein	NN
cord-104279-choywmwd	8	16	in	in	IN
cord-104279-choywmwd	8	17	these	these	DT
cord-104279-choywmwd	8	18	structures	structure	NNS
cord-104279-choywmwd	8	19	,	,	,
cord-104279-choywmwd	8	20	since	since	IN
cord-104279-choywmwd	8	21	replacement	replacement	NN
cord-104279-choywmwd	8	22	of	of	IN
cord-104279-choywmwd	8	23	the	the	DT
cord-104279-choywmwd	8	24	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	8	25	domain	domain	NN
cord-104279-choywmwd	8	26	of	of	IN
cord-104279-choywmwd	8	27	DPAP	DPAP	NNP
cord-104279-choywmwd	8	28	B	B	NNP
cord-104279-choywmwd	8	29	with	with	IN
cord-104279-choywmwd	8	30	that	that	DT
cord-104279-choywmwd	8	31	of	of	IN
cord-104279-choywmwd	8	32	DPAP	DPAP	NNP
cord-104279-choywmwd	8	33	A	A	NNP
cord-104279-choywmwd	8	34	resulted	result	VBD
cord-104279-choywmwd	8	35	in	in	IN
cord-104279-choywmwd	8	36	an	an	DT
cord-104279-choywmwd	8	37	immunolocalization	immunolocalization	NN
cord-104279-choywmwd	8	38	pattern	pattern	NN
cord-104279-choywmwd	8	39	indistinguishable	indistinguishable	JJ
cord-104279-choywmwd	8	40	from	from	IN
cord-104279-choywmwd	8	41	that	that	DT
cord-104279-choywmwd	8	42	of	of	IN
cord-104279-choywmwd	9	1	wild	wild	JJ
cord-104279-choywmwd	9	2	type	type	NN
cord-104279-choywmwd	9	3	DPAP	DPAP	NNP
cord-104279-choywmwd	9	4	A.	a.	NN
cord-104279-choywmwd	9	5	Overproduction	overproduction	NN
cord-104279-choywmwd	9	6	of	of	IN
cord-104279-choywmwd	9	7	DPAP	DPAP	NNP
cord-104279-choywmwd	9	8	A	A	NNP
cord-104279-choywmwd	9	9	resulted	result	VBD
cord-104279-choywmwd	9	10	in	in	IN
cord-104279-choywmwd	9	11	its	-PRON-	PRP$
cord-104279-choywmwd	9	12	mislocalization	mislocalization	NN
cord-104279-choywmwd	9	13	to	to	IN
cord-104279-choywmwd	9	14	the	the	DT
cord-104279-choywmwd	9	15	vacuole	vacuole	NN
cord-104279-choywmwd	9	16	,	,	,
cord-104279-choywmwd	9	17	because	because	IN
cord-104279-choywmwd	9	18	cells	cell	NNS
cord-104279-choywmwd	9	19	expressing	express	VBG
cord-104279-choywmwd	9	20	high	high	JJ
cord-104279-choywmwd	9	21	levels	level	NNS
cord-104279-choywmwd	9	22	of	of	IN
cord-104279-choywmwd	9	23	DPAP	DPAP	NNP
cord-104279-choywmwd	9	24	A	a	DT
cord-104279-choywmwd	9	25	exhibited	exhibit	VBN
cord-104279-choywmwd	9	26	vacuolar	vacuolar	NN
cord-104279-choywmwd	9	27	as	as	RB
cord-104279-choywmwd	9	28	well	well	RB
cord-104279-choywmwd	9	29	as	as	IN
cord-104279-choywmwd	9	30	Golgi	Golgi	NNP
cord-104279-choywmwd	9	31	staining	staining	NN
cord-104279-choywmwd	9	32	.	.	.
cord-104279-choywmwd	10	1	Deletion	deletion	NN
cord-104279-choywmwd	10	2	of	of	IN
cord-104279-choywmwd	10	3	22	22	CD
cord-104279-choywmwd	10	4	residues	residue	NNS
cord-104279-choywmwd	10	5	of	of	IN
cord-104279-choywmwd	10	6	the	the	DT
cord-104279-choywmwd	10	7	DPAP	DPAP	NNP
cord-104279-choywmwd	10	8	A	a	DT
cord-104279-choywmwd	10	9	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	10	10	domain	domain	NN
cord-104279-choywmwd	10	11	resulted	result	VBD
cord-104279-choywmwd	10	12	in	in	IN
cord-104279-choywmwd	10	13	mislocalization	mislocalization	NN
cord-104279-choywmwd	10	14	of	of	IN
cord-104279-choywmwd	10	15	the	the	DT
cord-104279-choywmwd	10	16	mutant	mutant	JJ
cord-104279-choywmwd	10	17	protein	protein	NN
cord-104279-choywmwd	10	18	to	to	IN
cord-104279-choywmwd	10	19	the	the	DT
cord-104279-choywmwd	10	20	vacuole	vacuole	NN
cord-104279-choywmwd	10	21	.	.	.
cord-104279-choywmwd	11	1	Thus	thus	RB
cord-104279-choywmwd	11	2	,	,	,
cord-104279-choywmwd	11	3	the	the	DT
cord-104279-choywmwd	11	4	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	11	5	domain	domain	NN
cord-104279-choywmwd	11	6	of	of	IN
cord-104279-choywmwd	11	7	DPAP	DPAP	NNP
cord-104279-choywmwd	11	8	A	A	NNP
cord-104279-choywmwd	11	9	is	be	VBZ
cord-104279-choywmwd	11	10	both	both	DT
cord-104279-choywmwd	11	11	necessary	necessary	JJ
cord-104279-choywmwd	11	12	and	and	CC
cord-104279-choywmwd	11	13	sufficient	sufficient	JJ
cord-104279-choywmwd	11	14	for	for	IN
cord-104279-choywmwd	11	15	Golgi	Golgi	NNP
cord-104279-choywmwd	11	16	retention	retention	NN
cord-104279-choywmwd	11	17	,	,	,
cord-104279-choywmwd	11	18	and	and	CC
cord-104279-choywmwd	11	19	removal	removal	NN
cord-104279-choywmwd	11	20	of	of	IN
cord-104279-choywmwd	11	21	the	the	DT
cord-104279-choywmwd	11	22	retention	retention	NN
cord-104279-choywmwd	11	23	signal	signal	NN
cord-104279-choywmwd	11	24	,	,	,
cord-104279-choywmwd	11	25	or	or	CC
cord-104279-choywmwd	11	26	saturation	saturation	NN
cord-104279-choywmwd	11	27	of	of	IN
cord-104279-choywmwd	11	28	the	the	DT
cord-104279-choywmwd	11	29	retention	retention	NN
cord-104279-choywmwd	11	30	apparatus	apparatus	NN
cord-104279-choywmwd	11	31	by	by	IN
cord-104279-choywmwd	11	32	overproducing	overproduce	VBG
cord-104279-choywmwd	11	33	DPAP	DPAP	NNP
cord-104279-choywmwd	11	34	A	A	NNP
cord-104279-choywmwd	11	35	,	,	,
cord-104279-choywmwd	11	36	resulted	result	VBD
cord-104279-choywmwd	11	37	in	in	IN
cord-104279-choywmwd	11	38	transport	transport	NN
cord-104279-choywmwd	11	39	to	to	IN
cord-104279-choywmwd	11	40	the	the	DT
cord-104279-choywmwd	11	41	vacuole	vacuole	NN
cord-104279-choywmwd	11	42	.	.	.
cord-104279-choywmwd	12	1	Like	like	IN
cord-104279-choywmwd	12	2	wild	wild	JJ
cord-104279-choywmwd	12	3	type	type	NN
cord-104279-choywmwd	12	4	DPAP	DPAP	NNP
cord-104279-choywmwd	12	5	B	B	NNP
cord-104279-choywmwd	12	6	,	,	,
cord-104279-choywmwd	12	7	the	the	DT
cord-104279-choywmwd	12	8	delivery	delivery	NN
cord-104279-choywmwd	12	9	of	of	IN
cord-104279-choywmwd	12	10	mutant	mutant	JJ
cord-104279-choywmwd	12	11	membrane	membrane	NN
cord-104279-choywmwd	12	12	proteins	protein	NNS
cord-104279-choywmwd	12	13	to	to	IN
cord-104279-choywmwd	12	14	the	the	DT
cord-104279-choywmwd	12	15	vacuole	vacuole	NN
cord-104279-choywmwd	12	16	was	be	VBD
cord-104279-choywmwd	12	17	unaffected	unaffected	JJ
cord-104279-choywmwd	12	18	in	in	IN
cord-104279-choywmwd	12	19	the	the	DT
cord-104279-choywmwd	12	20	secretory	secretory	JJ
cord-104279-choywmwd	12	21	vesicle	vesicle	NN
cord-104279-choywmwd	12	22	-	-	HYPH
cord-104279-choywmwd	12	23	blocked	block	VBN
cord-104279-choywmwd	12	24	sec1	sec1	JJ
cord-104279-choywmwd	12	25	mutant	mutant	NN
cord-104279-choywmwd	12	26	;	;	:
cord-104279-choywmwd	12	27	thus	thus	RB
cord-104279-choywmwd	12	28	,	,	,
cord-104279-choywmwd	12	29	transport	transport	NN
cord-104279-choywmwd	12	30	to	to	IN
cord-104279-choywmwd	12	31	the	the	DT
cord-104279-choywmwd	12	32	vacuole	vacuole	NN
cord-104279-choywmwd	12	33	was	be	VBD
cord-104279-choywmwd	12	34	not	not	RB
cord-104279-choywmwd	12	35	via	via	IN
cord-104279-choywmwd	12	36	the	the	DT
cord-104279-choywmwd	12	37	plasma	plasma	NN
cord-104279-choywmwd	12	38	membrane	membrane	NN
cord-104279-choywmwd	12	39	followed	follow	VBN
cord-104279-choywmwd	12	40	by	by	IN
cord-104279-choywmwd	12	41	endocytosis	endocytosis	NN
cord-104279-choywmwd	12	42	.	.	.
cord-104279-choywmwd	13	1	These	these	DT
cord-104279-choywmwd	13	2	data	datum	NNS
cord-104279-choywmwd	13	3	are	be	VBP
cord-104279-choywmwd	13	4	consistent	consistent	JJ
cord-104279-choywmwd	13	5	with	with	IN
cord-104279-choywmwd	13	6	a	a	DT
cord-104279-choywmwd	13	7	model	model	NN
cord-104279-choywmwd	13	8	in	in	IN
cord-104279-choywmwd	13	9	which	which	WDT
cord-104279-choywmwd	13	10	membrane	membrane	NN
cord-104279-choywmwd	13	11	proteins	protein	NNS
cord-104279-choywmwd	13	12	are	be	VBP
cord-104279-choywmwd	13	13	delivered	deliver	VBN
cord-104279-choywmwd	13	14	to	to	IN
cord-104279-choywmwd	13	15	the	the	DT
cord-104279-choywmwd	13	16	vacuole	vacuole	NN
cord-104279-choywmwd	13	17	along	along	IN
cord-104279-choywmwd	13	18	a	a	DT
cord-104279-choywmwd	13	19	default	default	NN
cord-104279-choywmwd	13	20	pathway	pathway	NN
cord-104279-choywmwd	13	21	.	.	.
cord-104279-choywmwd	14	1	Ar	Ar	NNP
cord-104279-choywmwd	15	1	~Y	~Y	NFP
cord-104279-choywmwd	15	2	proteins	protein	NNS
cord-104279-choywmwd	15	3	that	that	WDT
cord-104279-choywmwd	15	4	reside	reside	VBP
cord-104279-choywmwd	15	5	in	in	IN
cord-104279-choywmwd	15	6	the	the	DT
cord-104279-choywmwd	15	7	organelles	organelle	NNS
cord-104279-choywmwd	15	8	of	of	IN
cord-104279-choywmwd	15	9	the	the	DT
cord-104279-choywmwd	15	10	secretory	secretory	JJ
cord-104279-choywmwd	15	11	pathway	pathway	NN
cord-104279-choywmwd	15	12	of	of	IN
cord-104279-choywmwd	15	13	eukaryotic	eukaryotic	JJ
cord-104279-choywmwd	15	14	cells	cell	NNS
cord-104279-choywmwd	15	15	have	have	VBP
cord-104279-choywmwd	15	16	targeting	target	VBG
cord-104279-choywmwd	15	17	information	information	NN
cord-104279-choywmwd	15	18	that	that	WDT
cord-104279-choywmwd	15	19	directs	direct	VBZ
cord-104279-choywmwd	15	20	retention	retention	NN
cord-104279-choywmwd	15	21	in	in	IN
cord-104279-choywmwd	15	22	or	or	CC
cord-104279-choywmwd	15	23	sorting	sort	VBG
cord-104279-choywmwd	15	24	to	to	IN
cord-104279-choywmwd	15	25	the	the	DT
cord-104279-choywmwd	15	26	appropriate	appropriate	JJ
cord-104279-choywmwd	15	27	compartment	compartment	NN
cord-104279-choywmwd	15	28	(	(	-LRB-
cord-104279-choywmwd	15	29	Pfeffer	Pfeffer	NNP
cord-104279-choywmwd	15	30	and	and	CC
cord-104279-choywmwd	15	31	Rothman	Rothman	NNP
cord-104279-choywmwd	15	32	,	,	,
cord-104279-choywmwd	15	33	1987	1987	CD
cord-104279-choywmwd	15	34	)	)	-RRB-
cord-104279-choywmwd	15	35	.	.	.
cord-104279-choywmwd	16	1	In	in	IN
cord-104279-choywmwd	16	2	the	the	DT
cord-104279-choywmwd	16	3	absence	absence	NN
cord-104279-choywmwd	16	4	of	of	IN
cord-104279-choywmwd	16	5	retention	retention	NN
cord-104279-choywmwd	16	6	or	or	CC
cord-104279-choywmwd	16	7	sorting	sorting	NN
cord-104279-choywmwd	16	8	signals	signal	NNS
cord-104279-choywmwd	16	9	,	,	,
cord-104279-choywmwd	16	10	soluble	soluble	JJ
cord-104279-choywmwd	16	11	proteins	protein	NNS
cord-104279-choywmwd	16	12	of	of	IN
cord-104279-choywmwd	16	13	the	the	DT
cord-104279-choywmwd	16	14	secretory	secretory	JJ
cord-104279-choywmwd	16	15	pathway	pathway	NN
cord-104279-choywmwd	16	16	are	be	VBP
cord-104279-choywmwd	16	17	secreted	secrete	VBN
cord-104279-choywmwd	16	18	;	;	:
cord-104279-choywmwd	16	19	thus	thus	RB
cord-104279-choywmwd	16	20	,	,	,
cord-104279-choywmwd	16	21	the	the	DT
cord-104279-choywmwd	16	22	default	default	NN
cord-104279-choywmwd	16	23	pathway	pathway	NN
cord-104279-choywmwd	16	24	for	for	IN
cord-104279-choywmwd	16	25	these	these	DT
cord-104279-choywmwd	16	26	proteins	protein	NNS
cord-104279-choywmwd	16	27	is	be	VBZ
cord-104279-choywmwd	16	28	secretion	secretion	NN
cord-104279-choywmwd	16	29	(	(	-LRB-
cord-104279-choywmwd	16	30	Burgess	Burgess	NNP
cord-104279-choywmwd	16	31	and	and	CC
cord-104279-choywmwd	16	32	Kelly	Kelly	NNP
cord-104279-choywmwd	16	33	,	,	,
cord-104279-choywmwd	16	34	1987	1987	CD
cord-104279-choywmwd	16	35	;	;	:
cord-104279-choywmwd	16	36	Pelham	Pelham	NNP
cord-104279-choywmwd	16	37	,	,	,
cord-104279-choywmwd	16	38	1989	1989	CD
cord-104279-choywmwd	16	39	)	)	-RRB-
cord-104279-choywmwd	16	40	.	.	.
cord-104279-choywmwd	17	1	In	in	IN
cord-104279-choywmwd	17	2	Saccharomyces	Saccharomyces	NNP
cord-104279-choywmwd	17	3	cerevisiae	cerevisiae	NNS
cord-104279-choywmwd	17	4	,	,	,
cord-104279-choywmwd	17	5	mutations	mutation	NNS
cord-104279-choywmwd	17	6	in	in	IN
cord-104279-choywmwd	17	7	the	the	DT
cord-104279-choywmwd	17	8	targeting	targeting	JJ
cord-104279-choywmwd	17	9	signal	signal	NN
cord-104279-choywmwd	17	10	of	of	IN
cord-104279-choywmwd	17	11	the	the	DT
cord-104279-choywmwd	17	12	soluble	soluble	JJ
cord-104279-choywmwd	17	13	vacuolar	vacuolar	NNP
cord-104279-choywmwd	17	14	protein	protein	NN
cord-104279-choywmwd	17	15	,	,	,
cord-104279-choywmwd	17	16	carboxypeptidase	carboxypeptidase	NN
cord-104279-choywmwd	17	17	Y	Y	NNP
cord-104279-choywmwd	17	18	(	(	-LRB-
cord-104279-choywmwd	17	19	Vails	Vails	NNP
cord-104279-choywmwd	17	20	et	et	NNP
cord-104279-choywmwd	17	21	al	al	NNP
cord-104279-choywmwd	17	22	.	.	NNP
cord-104279-choywmwd	17	23	,	,	,
cord-104279-choywmwd	17	24	1990	1990	CD
cord-104279-choywmwd	17	25	)	)	-RRB-
cord-104279-choywmwd	17	26	,	,	,
cord-104279-choywmwd	17	27	or	or	CC
cord-104279-choywmwd	17	28	the	the	DT
cord-104279-choywmwd	17	29	retention	retention	NN
cord-104279-choywmwd	17	30	signal	signal	NN
cord-104279-choywmwd	17	31	of	of	IN
cord-104279-choywmwd	17	32	the	the	DT
cord-104279-choywmwd	17	33	soluble	soluble	JJ
cord-104279-choywmwd	17	34	ER	ER	NNP
cord-104279-choywmwd	17	35	protein	protein	NN
cord-104279-choywmwd	17	36	,	,	,
cord-104279-choywmwd	17	37	BiP	BiP	NNP
cord-104279-choywmwd	17	38	(	(	-LRB-
cord-104279-choywmwd	17	39	Hardwick	Hardwick	NNP
cord-104279-choywmwd	17	40	et	et	NNP
cord-104279-choywmwd	17	41	al	al	NNP
cord-104279-choywmwd	17	42	.	.	NNP
cord-104279-choywmwd	17	43	,	,	,
cord-104279-choywmwd	17	44	1990	1990	CD
cord-104279-choywmwd	17	45	)	)	-RRB-
cord-104279-choywmwd	17	46	,	,	,
cord-104279-choywmwd	17	47	result	result	VBP
cord-104279-choywmwd	17	48	in	in	IN
cord-104279-choywmwd	17	49	the	the	DT
cord-104279-choywmwd	17	50	secretion	secretion	NN
cord-104279-choywmwd	17	51	of	of	IN
cord-104279-choywmwd	17	52	these	these	DT
cord-104279-choywmwd	17	53	proteins	protein	NNS
cord-104279-choywmwd	17	54	.	.	.
cord-104279-choywmwd	18	1	Likewise	likewise	RB
cord-104279-choywmwd	18	2	,	,	,
cord-104279-choywmwd	18	3	the	the	DT
cord-104279-choywmwd	18	4	flow	flow	NN
cord-104279-choywmwd	18	5	of	of	IN
cord-104279-choywmwd	18	6	membrane	membrane	NN
cord-104279-choywmwd	18	7	proteins	protein	NNS
cord-104279-choywmwd	18	8	to	to	IN
cord-104279-choywmwd	18	9	the	the	DT
cord-104279-choywmwd	18	10	cell	cell	NN
cord-104279-choywmwd	18	11	surface	surface	NN
cord-104279-choywmwd	18	12	of	of	IN
cord-104279-choywmwd	18	13	nonpolarized	nonpolarize	VBN
cord-104279-choywmwd	18	14	mammalian	mammalian	JJ
cord-104279-choywmwd	18	15	cells	cell	NNS
cord-104279-choywmwd	18	16	is	be	VBZ
cord-104279-choywmwd	18	17	apparently	apparently	RB
cord-104279-choywmwd	18	18	by	by	IN
cord-104279-choywmwd	18	19	default	default	NN
cord-104279-choywmwd	18	20	,	,	,
cord-104279-choywmwd	18	21	because	because	IN
cord-104279-choywmwd	18	22	mutations	mutation	NNS
cord-104279-choywmwd	18	23	that	that	WDT
cord-104279-choywmwd	18	24	disrupt	disrupt	VBP
cord-104279-choywmwd	18	25	the	the	DT
cord-104279-choywmwd	18	26	retention	retention	NN
cord-104279-choywmwd	18	27	of	of	IN
cord-104279-choywmwd	18	28	ER	ER	NNP
cord-104279-choywmwd	18	29	or	or	CC
cord-104279-choywmwd	18	30	Golgi	Golgi	NNP
cord-104279-choywmwd	18	31	-	-	HYPH
cord-104279-choywmwd	18	32	retained	retain	VBN
cord-104279-choywmwd	18	33	membrane	membrane	NN
cord-104279-choywmwd	18	34	proteins	protein	NNS
cord-104279-choywmwd	19	1	(	(	-LRB-
cord-104279-choywmwd	19	2	Machamer	Machamer	NNP
cord-104279-choywmwd	19	3	et	et	NNP
cord-104279-choywmwd	19	4	al	al	NNP
cord-104279-choywmwd	19	5	.	.	NNP
cord-104279-choywmwd	19	6	,	,	,
cord-104279-choywmwd	19	7	1987	1987	CD
cord-104279-choywmwd	19	8	;	;	:
cord-104279-choywmwd	19	9	Machamer	Machamer	NNP
cord-104279-choywmwd	19	10	,	,	,
cord-104279-choywmwd	19	11	1991	1991	CD
cord-104279-choywmwd	19	12	;	;	:
cord-104279-choywmwd	19	13	Jackson	Jackson	NNP
cord-104279-choywmwd	19	14	et	et	NNP
cord-104279-choywmwd	19	15	al	al	NNP
cord-104279-choywmwd	19	16	.	.	NNP
cord-104279-choywmwd	19	17	,	,	,
cord-104279-choywmwd	19	18	1990	1990	CD
cord-104279-choywmwd	19	19	)	)	-RRB-
cord-104279-choywmwd	19	20	or	or	CC
cord-104279-choywmwd	19	21	the	the	DT
cord-104279-choywmwd	19	22	sorting	sorting	NN
cord-104279-choywmwd	19	23	of	of	IN
cord-104279-choywmwd	19	24	a	a	DT
cord-104279-choywmwd	19	25	lysosomal	lysosomal	JJ
cord-104279-choywmwd	19	26	membrane	membrane	NN
cord-104279-choywmwd	19	27	protein	protein	NN
cord-104279-choywmwd	19	28	(	(	-LRB-
cord-104279-choywmwd	19	29	Williams	Williams	NNP
cord-104279-choywmwd	19	30	and	and	CC
cord-104279-choywmwd	19	31	Fukuda	Fukuda	NNP
cord-104279-choywmwd	19	32	,	,	,
cord-104279-choywmwd	19	33	1990	1990	CD
cord-104279-choywmwd	19	34	)	)	-RRB-
cord-104279-choywmwd	19	35	result	result	VBP
cord-104279-choywmwd	19	36	in	in	IN
cord-104279-choywmwd	19	37	localization	localization	NN
cord-104279-choywmwd	19	38	to	to	IN
cord-104279-choywmwd	19	39	the	the	DT
cord-104279-choywmwd	19	40	plasma	plasma	NN
cord-104279-choywmwd	19	41	membrane	membrane	NN
cord-104279-choywmwd	19	42	.	.	.
cord-104279-choywmwd	20	1	Little	little	JJ
cord-104279-choywmwd	20	2	is	be	VBZ
cord-104279-choywmwd	20	3	known	know	VBN
cord-104279-choywmwd	20	4	regarding	regard	VBG
cord-104279-choywmwd	20	5	membrane	membrane	NN
cord-104279-choywmwd	20	6	protein	protein	NN
cord-104279-choywmwd	20	7	sorting	sorting	NN
cord-104279-choywmwd	20	8	in	in	IN
cord-104279-choywmwd	20	9	S.	S.	NNP
cord-104279-choywmwd	20	10	cerevisiae	cerevisiae	NNS
cord-104279-choywmwd	20	11	,	,	,
cord-104279-choywmwd	20	12	although	although	IN
cord-104279-choywmwd	20	13	a	a	DT
cord-104279-choywmwd	20	14	previous	previous	JJ
cord-104279-choywmwd	20	15	study	study	NN
cord-104279-choywmwd	20	16	suggested	suggest	VBD
cord-104279-choywmwd	20	17	that	that	IN
cord-104279-choywmwd	20	18	the	the	DT
cord-104279-choywmwd	20	19	cell	cell	NN
cord-104279-choywmwd	20	20	surface	surface	NN
cord-104279-choywmwd	20	21	is	be	VBZ
cord-104279-choywmwd	20	22	the	the	DT
cord-104279-choywmwd	20	23	default	default	NN
cord-104279-choywmwd	20	24	compartment	compartment	NN
cord-104279-choywmwd	20	25	for	for	IN
cord-104279-choywmwd	20	26	membrane	membrane	NN
cord-104279-choywmwd	20	27	proteins	protein	NNS
cord-104279-choywmwd	21	1	(	(	-LRB-
cord-104279-choywmwd	21	2	Fuller	Fuller	NNP
cord-104279-choywmwd	21	3	et	et	NNP
cord-104279-choywmwd	21	4	al	al	NNP
cord-104279-choywmwd	21	5	.	.	NNP
cord-104279-choywmwd	21	6	,	,	,
cord-104279-choywmwd	21	7	1989b	1989b	CD
cord-104279-choywmwd	21	8	)	)	-RRB-
cord-104279-choywmwd	21	9	.	.	.
cord-104279-choywmwd	22	1	In	in	IN
cord-104279-choywmwd	22	2	this	this	DT
cord-104279-choywmwd	22	3	paper	paper	NN
cord-104279-choywmwd	22	4	,	,	,
cord-104279-choywmwd	22	5	we	-PRON-	PRP
cord-104279-choywmwd	22	6	characterize	characterize	VBP
cord-104279-choywmwd	22	7	the	the	DT
cord-104279-choywmwd	22	8	targeting	targeting	NN
cord-104279-choywmwd	22	9	of	of	IN
cord-104279-choywmwd	22	10	two	two	CD
cord-104279-choywmwd	22	11	membrane	membrane	NN
cord-104279-choywmwd	22	12	proteins	protein	NNS
cord-104279-choywmwd	22	13	of	of	IN
cord-104279-choywmwd	22	14	the	the	DT
cord-104279-choywmwd	22	15	yeast	yeast	NN
cord-104279-choywmwd	22	16	secretory	secretory	NN
cord-104279-choywmwd	22	17	pathway	pathway	NN
cord-104279-choywmwd	22	18	,	,	.
cord-104279-choywmwd	23	1	DAP	DAP	NNP
cord-104279-choywmwd	23	2	dipeptidyl	dipeptidyl	NNP
cord-104279-choywmwd	23	3	aminopeptidases	aminopeptidases	NNPS
cord-104279-choywmwd	23	4	(	(	-LRB-
cord-104279-choywmwd	23	5	DPAPs	DPAPs	NNP
cord-104279-choywmwd	23	6	)	)	-RRB-
cord-104279-choywmwd	23	7	~	~	NFP
cord-104279-choywmwd	24	1	DPAP	DPAP	NNP
cord-104279-choywmwd	24	2	A	A	NNP
cord-104279-choywmwd	24	3	and	and	CC
cord-104279-choywmwd	24	4	DPAP	DPAP	NNP
cord-104279-choywmwd	24	5	B	B	NNP
cord-104279-choywmwd	24	6	of	of	IN
cord-104279-choywmwd	24	7	the	the	DT
cord-104279-choywmwd	24	8	Golgi	Golgi	NNP
cord-104279-choywmwd	24	9	apparatus	apparatus	NN
cord-104279-choywmwd	24	10	and	and	CC
cord-104279-choywmwd	24	11	vacuole	vacuole	NN
cord-104279-choywmwd	24	12	,	,	,
cord-104279-choywmwd	24	13	respectively	respectively	RB
cord-104279-choywmwd	24	14	.	.	.
cord-104279-choywmwd	25	1	The	the	DT
cord-104279-choywmwd	25	2	biogenesis	biogenesis	NN
cord-104279-choywmwd	25	3	of	of	IN
cord-104279-choywmwd	25	4	two	two	CD
cord-104279-choywmwd	25	5	membrane	membrane	NN
cord-104279-choywmwd	25	6	proteins	protein	NNS
cord-104279-choywmwd	25	7	of	of	IN
cord-104279-choywmwd	25	8	the	the	DT
cord-104279-choywmwd	25	9	yeast	yeast	NN
cord-104279-choywmwd	25	10	vacuole	vacuole	NN
cord-104279-choywmwd	25	11	,	,	,
cord-104279-choywmwd	25	12	DPAP	DPAP	NNP
cord-104279-choywmwd	25	13	B	B	NNP
cord-104279-choywmwd	25	14	(	(	-LRB-
cord-104279-choywmwd	25	15	see	see	VB
cord-104279-choywmwd	25	16	Fig	Fig	NNP
cord-104279-choywmwd	25	17	.	.	.
cord-104279-choywmwd	25	18	1	1	CD
cord-104279-choywmwd	25	19	A	a	CD
cord-104279-choywmwd	25	20	)	)	-RRB-
cord-104279-choywmwd	25	21	,	,	,
cord-104279-choywmwd	25	22	and	and	CC
cord-104279-choywmwd	25	23	alkaline	alkaline	JJ
cord-104279-choywmwd	25	24	phosphatase	phosphatase	NN
cord-104279-choywmwd	25	25	(	(	-LRB-
cord-104279-choywmwd	25	26	ALP	ALP	NNP
cord-104279-choywmwd	25	27	)	)	-RRB-
cord-104279-choywmwd	25	28	,	,	,
cord-104279-choywmwd	25	29	has	have	VBZ
cord-104279-choywmwd	25	30	been	be	VBN
cord-104279-choywmwd	25	31	characterized	characterize	VBN
cord-104279-choywmwd	25	32	(	(	-LRB-
cord-104279-choywmwd	25	33	Klionsky	Klionsky	NNP
cord-104279-choywmwd	25	34	and	and	CC
cord-104279-choywmwd	25	35	Emr	Emr	NNP
cord-104279-choywmwd	25	36	,	,	,
cord-104279-choywmwd	25	37	1989	1989	CD
cord-104279-choywmwd	25	38	;	;	:
cord-104279-choywmwd	25	39	Roberts	Roberts	NNP
cord-104279-choywmwd	25	40	et	et	NNP
cord-104279-choywmwd	25	41	al	al	NNP
cord-104279-choywmwd	25	42	.	.	NNP
cord-104279-choywmwd	25	43	,	,	,
cord-104279-choywmwd	25	44	1989	1989	CD
cord-104279-choywmwd	25	45	)	)	-RRB-
cord-104279-choywmwd	25	46	.	.	.
cord-104279-choywmwd	26	1	Both	both	DT
cord-104279-choywmwd	26	2	DPAP	DPAP	NNP
cord-104279-choywmwd	26	3	B	B	NNP
cord-104279-choywmwd	26	4	and	and	CC
cord-104279-choywmwd	26	5	ALP	ALP	NNP
cord-104279-choywmwd	26	6	are	be	VBP
cord-104279-choywmwd	26	7	type	type	NN
cord-104279-choywmwd	26	8	II	II	NNP
cord-104279-choywmwd	26	9	membrane	membrane	NN
cord-104279-choywmwd	26	10	glycoproteins	glycoprotein	NNS
cord-104279-choywmwd	26	11	(	(	-LRB-
cord-104279-choywmwd	26	12	nomenclature	nomenclature	NN
cord-104279-choywmwd	26	13	of	of	IN
cord-104279-choywmwd	26	14	Singer	Singer	NNP
cord-104279-choywmwd	26	15	,	,	,
cord-104279-choywmwd	26	16	1990	1990	CD
cord-104279-choywmwd	26	17	)	)	-RRB-
cord-104279-choywmwd	26	18	,	,	,
cord-104279-choywmwd	26	19	consisting	consist	VBG
cord-104279-choywmwd	26	20	of	of	IN
cord-104279-choywmwd	26	21	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	26	22	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	26	23	domains	domain	NNS
cord-104279-choywmwd	26	24	of	of	IN
cord-104279-choywmwd	26	25	approximately	approximately	RB
cord-104279-choywmwd	26	26	30	30	CD
cord-104279-choywmwd	26	27	amino	amino	JJ
cord-104279-choywmwd	26	28	acids	acid	NNS
cord-104279-choywmwd	26	29	,	,	,
cord-104279-choywmwd	26	30	single	single	JJ
cord-104279-choywmwd	26	31	hydrophobic	hydrophobic	JJ
cord-104279-choywmwd	26	32	membrane	membrane	NN
cord-104279-choywmwd	26	33	anchors	anchor	NNS
cord-104279-choywmwd	26	34	,	,	,
cord-104279-choywmwd	26	35	and	and	CC
cord-104279-choywmwd	26	36	COOH	COOH	NNP
cord-104279-choywmwd	26	37	-	-	HYPH
cord-104279-choywmwd	26	38	terminal	terminal	JJ
cord-104279-choywmwd	26	39	lumenal	lumenal	JJ
cord-104279-choywmwd	26	40	catalytic	catalytic	JJ
cord-104279-choywmwd	26	41	domains	domain	NNS
cord-104279-choywmwd	26	42	.	.	.
cord-104279-choywmwd	27	1	These	these	DT
cord-104279-choywmwd	27	2	proteins	protein	NNS
cord-104279-choywmwd	27	3	transit	transit	VBP
cord-104279-choywmwd	27	4	the	the	DT
cord-104279-choywmwd	27	5	early	early	JJ
cord-104279-choywmwd	27	6	compartments	compartment	NNS
cord-104279-choywmwd	27	7	of	of	IN
cord-104279-choywmwd	27	8	the	the	DT
cord-104279-choywmwd	27	9	secretory	secretory	JJ
cord-104279-choywmwd	27	10	pathway	pathway	NN
cord-104279-choywmwd	27	11	(	(	-LRB-
cord-104279-choywmwd	27	12	i.e.	i.e.	FW
cord-104279-choywmwd	27	13	,	,	,
cord-104279-choywmwd	27	14	ER	ER	NNP
cord-104279-choywmwd	27	15	and	and	CC
cord-104279-choywmwd	27	16	Golgi	Golgi	NNP
cord-104279-choywmwd	27	17	)	)	-RRB-
cord-104279-choywmwd	27	18	,	,	,
cord-104279-choywmwd	27	19	but	but	CC
cord-104279-choywmwd	27	20	not	not	RB
cord-104279-choywmwd	27	21	the	the	DT
cord-104279-choywmwd	27	22	later	later	JJ
cord-104279-choywmwd	27	23	compartments	compartment	NNS
cord-104279-choywmwd	27	24	(	(	-LRB-
cord-104279-choywmwd	27	25	i.e.	i.e.	FW
cord-104279-choywmwd	27	26	,	,	,
cord-104279-choywmwd	27	27	secretory	secretory	JJ
cord-104279-choywmwd	27	28	vesicles	vesicle	NNS
cord-104279-choywmwd	27	29	)	)	-RRB-
cord-104279-choywmwd	27	30	,	,	,
cord-104279-choywmwd	27	31	indicating	indicate	VBG
cord-104279-choywmwd	27	32	that	that	IN
cord-104279-choywmwd	27	33	the	the	DT
cord-104279-choywmwd	27	34	proteins	protein	NNS
cord-104279-choywmwd	27	35	do	do	VBP
cord-104279-choywmwd	27	36	not	not	RB
cord-104279-choywmwd	27	37	transiently	transiently	RB
cord-104279-choywmwd	27	38	reside	reside	VB
cord-104279-choywmwd	27	39	at	at	IN
cord-104279-choywmwd	27	40	the	the	DT
cord-104279-choywmwd	27	41	plasma	plasma	NN
cord-104279-choywmwd	27	42	membrane	membrane	NN
cord-104279-choywmwd	27	43	before	before	IN
cord-104279-choywmwd	27	44	delivery	delivery	NN
cord-104279-choywmwd	27	45	to	to	IN
cord-104279-choywmwd	27	46	the	the	DT
cord-104279-choywmwd	27	47	vacuole	vacuole	NN
cord-104279-choywmwd	27	48	.	.	.
cord-104279-choywmwd	28	1	The	the	DT
cord-104279-choywmwd	28	2	localization	localization	NN
cord-104279-choywmwd	28	3	signals	signal	NNS
cord-104279-choywmwd	28	4	of	of	IN
cord-104279-choywmwd	28	5	these	these	DT
cord-104279-choywmwd	28	6	two	two	CD
cord-104279-choywmwd	28	7	proteins	protein	NNS
cord-104279-choywmwd	28	8	have	have	VBP
cord-104279-choywmwd	28	9	not	not	RB
cord-104279-choywmwd	28	10	been	be	VBN
cord-104279-choywmwd	28	11	identified	identify	VBN
cord-104279-choywmwd	28	12	,	,	,
cord-104279-choywmwd	28	13	although	although	IN
cord-104279-choywmwd	28	14	the	the	DT
cord-104279-choywmwd	28	15	lumenal	lumenal	JJ
cord-104279-choywmwd	28	16	domain	domain	NN
cord-104279-choywmwd	28	17	of	of	IN
cord-104279-choywmwd	28	18	ALP	ALP	NNP
cord-104279-choywmwd	28	19	has	have	VBZ
cord-104279-choywmwd	28	20	been	be	VBN
cord-104279-choywmwd	28	21	shown	show	VBN
cord-104279-choywmwd	28	22	to	to	TO
cord-104279-choywmwd	28	23	be	be	VB
cord-104279-choywmwd	28	24	unnecessary	unnecessary	JJ
cord-104279-choywmwd	28	25	for	for	IN
cord-104279-choywmwd	28	26	vacuolar	vacuolar	NNP
cord-104279-choywmwd	28	27	targeting	target	VBG
cord-104279-choywmwd	28	28	(	(	-LRB-
cord-104279-choywmwd	28	29	Klionsky	Klionsky	NNP
cord-104279-choywmwd	28	30	and	and	CC
cord-104279-choywmwd	28	31	Emr	Emr	NNP
cord-104279-choywmwd	28	32	,	,	,
cord-104279-choywmwd	28	33	1990	1990	CD
cord-104279-choywmwd	28	34	)	)	-RRB-
cord-104279-choywmwd	28	35	.	.	.
cord-104279-choywmwd	29	1	The	the	DT
cord-104279-choywmwd	29	2	biosynthesis	biosynthesis	NN
cord-104279-choywmwd	29	3	of	of	IN
cord-104279-choywmwd	29	4	several	several	JJ
cord-104279-choywmwd	29	5	membrane	membrane	NN
cord-104279-choywmwd	29	6	proteins	protein	NNS
cord-104279-choywmwd	29	7	that	that	WDT
cord-104279-choywmwd	29	8	reside	reside	VBP
cord-104279-choywmwd	29	9	This	this	DT
cord-104279-choywmwd	29	10	paper	paper	NN
cord-104279-choywmwd	29	11	is	be	VBZ
cord-104279-choywmwd	29	12	dedicated	dedicate	VBN
cord-104279-choywmwd	29	13	to	to	IN
cord-104279-choywmwd	29	14	the	the	DT
cord-104279-choywmwd	29	15	memory	memory	NN
cord-104279-choywmwd	29	16	of	of	IN
cord-104279-choywmwd	29	17	David	David	NNP
cord-104279-choywmwd	29	18	Merrill	Merrill	NNP
cord-104279-choywmwd	29	19	Stevens	Stevens	NNP
cord-104279-choywmwd	29	20	.	.	.
cord-104279-choywmwd	30	1	in	in	IN
cord-104279-choywmwd	30	2	the	the	DT
cord-104279-choywmwd	30	3	yeast	yeast	NN
cord-104279-choywmwd	30	4	Golgi	Golgi	NNP
cord-104279-choywmwd	30	5	apparatus	apparatus	NN
cord-104279-choywmwd	30	6	has	have	VBZ
cord-104279-choywmwd	30	7	also	also	RB
cord-104279-choywmwd	30	8	been	be	VBN
cord-104279-choywmwd	30	9	examined	examine	VBN
cord-104279-choywmwd	30	10	.	.	.
cord-104279-choywmwd	31	1	In	in	IN
cord-104279-choywmwd	31	2	yeast	yeast	NN
cord-104279-choywmwd	31	3	,	,	,
cord-104279-choywmwd	31	4	three	three	CD
cord-104279-choywmwd	31	5	membrane	membrane	NN
cord-104279-choywmwd	31	6	-	-	HYPH
cord-104279-choywmwd	31	7	bound	bind	VBN
cord-104279-choywmwd	31	8	proteases	protease	NNS
cord-104279-choywmwd	31	9	,	,	,
cord-104279-choywmwd	31	10	Kex2p	Kex2p	NNP
cord-104279-choywmwd	31	11	,	,	,
cord-104279-choywmwd	31	12	Kexlp	Kexlp	NNP
cord-104279-choywmwd	31	13	,	,	,
cord-104279-choywmwd	31	14	and	and	CC
cord-104279-choywmwd	31	15	DPAP	DPAP	NNP
cord-104279-choywmwd	31	16	A	A	NNP
cord-104279-choywmwd	31	17	(	(	-LRB-
cord-104279-choywmwd	31	18	see	see	VB
cord-104279-choywmwd	31	19	Fig	Fig	NNP
cord-104279-choywmwd	31	20	.	.	.
cord-104279-choywmwd	31	21	1	1	CD
cord-104279-choywmwd	31	22	A	a	CD
cord-104279-choywmwd	31	23	)	)	-RRB-
cord-104279-choywmwd	31	24	process	process	NN
cord-104279-choywmwd	31	25	the	the	DT
cord-104279-choywmwd	31	26	mating	mating	NN
cord-104279-choywmwd	31	27	pheromone	pheromone	NN
cord-104279-choywmwd	31	28	a	a	DT
cord-104279-choywmwd	31	29	-	-	HYPH
cord-104279-choywmwd	31	30	factor	factor	NN
cord-104279-choywmwd	31	31	precursor	precursor	NN
cord-104279-choywmwd	31	32	polypeptide	polypeptide	NN
cord-104279-choywmwd	31	33	as	as	IN
cord-104279-choywmwd	31	34	it	-PRON-	PRP
cord-104279-choywmwd	31	35	traverses	traverse	VBZ
cord-104279-choywmwd	31	36	the	the	DT
cord-104279-choywmwd	31	37	secretory	secretory	JJ
cord-104279-choywmwd	31	38	pathway	pathway	NN
cord-104279-choywmwd	31	39	(	(	-LRB-
cord-104279-choywmwd	31	40	Bussey	Bussey	NNP
cord-104279-choywmwd	31	41	,	,	,
cord-104279-choywmwd	31	42	1988	1988	CD
cord-104279-choywmwd	31	43	;	;	.
cord-104279-choywmwd	32	1	Fuller	Fuller	NNP
cord-104279-choywmwd	32	2	et	et	NNP
cord-104279-choywmwd	32	3	al	al	NNP
cord-104279-choywmwd	32	4	.	.	NNP
cord-104279-choywmwd	32	5	,	,	,
cord-104279-choywmwd	32	6	1988	1988	CD
cord-104279-choywmwd	32	7	)	)	-RRB-
cord-104279-choywmwd	32	8	.	.	.
cord-104279-choywmwd	33	1	The	the	DT
cord-104279-choywmwd	33	2	biosynthetic	biosynthetic	JJ
cord-104279-choywmwd	33	3	pathways	pathway	NNS
cord-104279-choywmwd	33	4	of	of	IN
cord-104279-choywmwd	33	5	Kex2p	Kex2p	NNP
cord-104279-choywmwd	33	6	and	and	CC
cord-104279-choywmwd	33	7	Kexlp	Kexlp	NNP
cord-104279-choywmwd	33	8	have	have	VBP
cord-104279-choywmwd	33	9	been	be	VBN
cord-104279-choywmwd	33	10	characterized	characterize	VBN
cord-104279-choywmwd	34	1	(	(	-LRB-
cord-104279-choywmwd	34	2	Fuller	Fuller	NNP
cord-104279-choywmwd	34	3	et	et	NNP
cord-104279-choywmwd	34	4	al	al	NNP
cord-104279-choywmwd	34	5	.	.	NNP
cord-104279-choywmwd	34	6	,	,	,
cord-104279-choywmwd	34	7	1989a	1989a	CD
cord-104279-choywmwd	34	8	,	,	,
cord-104279-choywmwd	34	9	b	b	LS
cord-104279-choywmwd	34	10	;	;	:
cord-104279-choywmwd	34	11	Cooper	Cooper	NNP
cord-104279-choywmwd	34	12	and	and	CC
cord-104279-choywmwd	34	13	Bussey	Bussey	NNP
cord-104279-choywmwd	34	14	,	,	,
cord-104279-choywmwd	34	15	1989	1989	CD
cord-104279-choywmwd	34	16	)	)	-RRB-
cord-104279-choywmwd	34	17	,	,	,
cord-104279-choywmwd	34	18	and	and	CC
cord-104279-choywmwd	34	19	Kex2p	Kex2p	NNP
cord-104279-choywmwd	34	20	has	have	VBZ
cord-104279-choywmwd	34	21	been	be	VBN
cord-104279-choywmwd	34	22	shown	show	VBN
cord-104279-choywmwd	34	23	to	to	TO
cord-104279-choywmwd	34	24	function	function	VB
cord-104279-choywmwd	34	25	in	in	IN
cord-104279-choywmwd	34	26	a	a	DT
cord-104279-choywmwd	34	27	late	late	JJ
cord-104279-choywmwd	34	28	Golgi	Golgi	NNP
cord-104279-choywmwd	34	29	compartment	compartment	NN
cord-104279-choywmwd	34	30	(	(	-LRB-
cord-104279-choywmwd	34	31	Julius	Julius	NNP
cord-104279-choywmwd	34	32	et	et	NNP
cord-104279-choywmwd	34	33	al	al	NNP
cord-104279-choywmwd	34	34	.	.	NNP
cord-104279-choywmwd	34	35	,	,	,
cord-104279-choywmwd	34	36	1984	1984	CD
cord-104279-choywmwd	34	37	;	;	:
cord-104279-choywmwd	34	38	Graham	Graham	NNP
cord-104279-choywmwd	34	39	and	and	CC
cord-104279-choywmwd	34	40	Emr	Emr	NNP
cord-104279-choywmwd	34	41	,	,	,
cord-104279-choywmwd	34	42	1991	1991	CD
cord-104279-choywmwd	34	43	)	)	-RRB-
cord-104279-choywmwd	34	44	.	.	.
cord-104279-choywmwd	35	1	Kex2p	Kex2p	NNP
cord-104279-choywmwd	35	2	has	have	VBZ
cord-104279-choywmwd	35	3	been	be	VBN
cord-104279-choywmwd	35	4	localized	localize	VBN
cord-104279-choywmwd	35	5	by	by	IN
cord-104279-choywmwd	35	6	indirect	indirect	JJ
cord-104279-choywmwd	35	7	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	35	8	to	to	IN
cord-104279-choywmwd	35	9	three	three	CD
cord-104279-choywmwd	35	10	to	to	TO
cord-104279-choywmwd	35	11	six	six	CD
cord-104279-choywmwd	35	12	punctate	punctate	NN
cord-104279-choywmwd	35	13	structures	structure	NNS
cord-104279-choywmwd	35	14	per	per	IN
cord-104279-choywmwd	35	15	cell	cell	NN
cord-104279-choywmwd	35	16	that	that	WDT
cord-104279-choywmwd	35	17	exhibit	exhibit	VBP
cord-104279-choywmwd	35	18	a	a	DT
cord-104279-choywmwd	35	19	somewhat	somewhat	RB
cord-104279-choywmwd	35	20	random	random	JJ
cord-104279-choywmwd	35	21	distribution	distribution	NN
cord-104279-choywmwd	35	22	within	within	IN
cord-104279-choywmwd	35	23	the	the	DT
cord-104279-choywmwd	35	24	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	35	25	compartment	compartment	NN
cord-104279-choywmwd	35	26	(	(	-LRB-
cord-104279-choywmwd	35	27	Franzusoff	Franzusoff	NNP
cord-104279-choywmwd	35	28	et	et	NNP
cord-104279-choywmwd	35	29	al	al	NNP
cord-104279-choywmwd	35	30	.	.	NNP
cord-104279-choywmwd	35	31	,	,	,
cord-104279-choywmwd	35	32	1991	1991	CD
cord-104279-choywmwd	35	33	;	;	:
cord-104279-choywmwd	35	34	Redding	Redding	NNP
cord-104279-choywmwd	35	35	et	et	NNP
cord-104279-choywmwd	35	36	al	al	NNP
cord-104279-choywmwd	35	37	.	.	NNP
cord-104279-choywmwd	35	38	,	,	,
cord-104279-choywmwd	35	39	1991	1991	CD
cord-104279-choywmwd	35	40	)	)	-RRB-
cord-104279-choywmwd	35	41	.	.	.
cord-104279-choywmwd	36	1	Thus	thus	RB
cord-104279-choywmwd	36	2	,	,	,
cord-104279-choywmwd	36	3	the	the	DT
cord-104279-choywmwd	36	4	Golgi	Golgi	NNP
cord-104279-choywmwd	36	5	apparatus	apparatus	NN
cord-104279-choywmwd	36	6	of	of	IN
cord-104279-choywmwd	36	7	yeast	yeast	NN
cord-104279-choywmwd	36	8	is	be	VBZ
cord-104279-choywmwd	36	9	not	not	RB
cord-104279-choywmwd	36	10	localized	localize	VBN
cord-104279-choywmwd	36	11	to	to	IN
cord-104279-choywmwd	36	12	a	a	DT
cord-104279-choywmwd	36	13	perinuclear	perinuclear	JJ
cord-104279-choywmwd	36	14	location	location	NN
cord-104279-choywmwd	36	15	,	,	,
cord-104279-choywmwd	36	16	as	as	IN
cord-104279-choywmwd	36	17	in	in	IN
cord-104279-choywmwd	36	18	mammalian	mammalian	JJ
cord-104279-choywmwd	36	19	cells	cell	NNS
cord-104279-choywmwd	36	20	,	,	,
cord-104279-choywmwd	36	21	but	but	CC
cord-104279-choywmwd	36	22	rather	rather	RB
cord-104279-choywmwd	36	23	is	be	VBZ
cord-104279-choywmwd	36	24	dispersed	disperse	VBN
cord-104279-choywmwd	36	25	throughout	throughout	IN
cord-104279-choywmwd	36	26	the	the	DT
cord-104279-choywmwd	36	27	cell	cell	NN
cord-104279-choywmwd	36	28	.	.	.
cord-104279-choywmwd	37	1	Both	both	DT
cord-104279-choywmwd	37	2	Kexlp	Kexlp	NNP
cord-104279-choywmwd	37	3	(	(	-LRB-
cord-104279-choywmwd	37	4	A.	A.	NNP
cord-104279-choywmwd	37	5	Cooper	Cooper	NNP
cord-104279-choywmwd	37	6	and	and	CC
cord-104279-choywmwd	37	7	H.	H.	NNP
cord-104279-choywmwd	37	8	Bussey	Bussey	NNP
cord-104279-choywmwd	37	9	,	,	,
cord-104279-choywmwd	37	10	manuscript	manuscript	NN
cord-104279-choywmwd	37	11	submitted	submit	VBD
cord-104279-choywmwd	37	12	)	)	-RRB-
cord-104279-choywmwd	37	13	and	and	CC
cord-104279-choywmwd	37	14	DPAP	DPAP	NNP
cord-104279-choywmwd	37	15	A	A	NNP
cord-104279-choywmwd	37	16	(	(	-LRB-
cord-104279-choywmwd	37	17	see	see	VB
cord-104279-choywmwd	37	18	below	below	RB
cord-104279-choywmwd	37	19	)	)	-RRB-
cord-104279-choywmwd	37	20	have	have	VBP
cord-104279-choywmwd	37	21	been	be	VBN
cord-104279-choywmwd	37	22	immunolocalized	immunolocalize	VBN
cord-104279-choywmwd	37	23	to	to	TO
cord-104279-choywmwd	37	24	punctate	punctate	VB
cord-104279-choywmwd	37	25	bodies	body	NNS
cord-104279-choywmwd	37	26	that	that	WDT
cord-104279-choywmwd	37	27	are	be	VBP
cord-104279-choywmwd	37	28	similar	similar	JJ
cord-104279-choywmwd	37	29	to	to	IN
cord-104279-choywmwd	37	30	those	those	DT
cord-104279-choywmwd	37	31	containing	contain	VBG
cord-104279-choywmwd	37	32	Kex2p	Kex2p	NNP
cord-104279-choywmwd	37	33	in	in	IN
cord-104279-choywmwd	37	34	size	size	NN
cord-104279-choywmwd	37	35	,	,	,
cord-104279-choywmwd	37	36	abundance	abundance	NN
cord-104279-choywmwd	37	37	,	,	,
cord-104279-choywmwd	37	38	and	and	CC
cord-104279-choywmwd	37	39	distribution	distribution	NN
cord-104279-choywmwd	37	40	.	.	.
cord-104279-choywmwd	38	1	In	in	IN
cord-104279-choywmwd	38	2	this	this	DT
cord-104279-choywmwd	38	3	paper	paper	NN
cord-104279-choywmwd	38	4	,	,	,
cord-104279-choywmwd	38	5	a	a	DT
cord-104279-choywmwd	38	6	combined	combine	VBN
cord-104279-choywmwd	38	7	gene	gene	NN
cord-104279-choywmwd	38	8	fusion	fusion	NN
cord-104279-choywmwd	38	9	and	and	CC
cord-104279-choywmwd	38	10	mutational	mutational	JJ
cord-104279-choywmwd	38	11	analysis	analysis	NN
cord-104279-choywmwd	38	12	was	be	VBD
cord-104279-choywmwd	38	13	used	use	VBN
cord-104279-choywmwd	38	14	to	to	TO
cord-104279-choywmwd	38	15	show	show	VB
cord-104279-choywmwd	38	16	that	that	IN
cord-104279-choywmwd	38	17	no	no	DT
cord-104279-choywmwd	38	18	single	single	JJ
cord-104279-choywmwd	38	19	domain	domain	NN
cord-104279-choywmwd	38	20	of	of	IN
cord-104279-choywmwd	38	21	DPAP	DPAP	NNP
cord-104279-choywmwd	38	22	B	B	NNP
cord-104279-choywmwd	38	23	is	be	VBZ
cord-104279-choywmwd	38	24	required	require	VBN
cord-104279-choywmwd	38	25	for	for	IN
cord-104279-choywmwd	38	26	vacuolar	vacuolar	NNP
cord-104279-choywmwd	38	27	localization	localization	NN
cord-104279-choywmwd	38	28	.	.	.
cord-104279-choywmwd	39	1	Furthermore	furthermore	RB
cord-104279-choywmwd	39	2	,	,	,
cord-104279-choywmwd	39	3	both	both	DT
cord-104279-choywmwd	39	4	overproduction	overproduction	NN
cord-104279-choywmwd	39	5	of	of	IN
cord-104279-choywmwd	39	6	DPAP	DPAP	NNP
cord-104279-choywmwd	39	7	A	A	NNP
cord-104279-choywmwd	39	8	and	and	CC
cord-104279-choywmwd	39	9	a	a	DT
cord-104279-choywmwd	39	10	mutation	mutation	NN
cord-104279-choywmwd	39	11	in	in	IN
cord-104279-choywmwd	39	12	the	the	DT
cord-104279-choywmwd	39	13	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	39	14	domain	domain	NN
cord-104279-choywmwd	39	15	of	of	IN
cord-104279-choywmwd	39	16	DPAP	DPAP	NNP
cord-104279-choywmwd	39	17	A	A	NNP
cord-104279-choywmwd	39	18	resulted	result	VBD
cord-104279-choywmwd	39	19	in	in	IN
cord-104279-choywmwd	39	20	mislocalization	mislocalization	NN
cord-104279-choywmwd	39	21	of	of	IN
cord-104279-choywmwd	39	22	this	this	DT
cord-104279-choywmwd	39	23	protein	protein	NN
cord-104279-choywmwd	39	24	to	to	IN
cord-104279-choywmwd	39	25	the	the	DT
cord-104279-choywmwd	39	26	vacuole	vacuole	NN
cord-104279-choywmwd	39	27	.	.	.
cord-104279-choywmwd	40	1	Finally	finally	RB
cord-104279-choywmwd	40	2	,	,	,
cord-104279-choywmwd	40	3	the	the	DT
cord-104279-choywmwd	40	4	fusion	fusion	NN
cord-104279-choywmwd	40	5	proteins	protein	NNS
cord-104279-choywmwd	40	6	analyzed	analyze	VBN
cord-104279-choywmwd	40	7	in	in	IN
cord-104279-choywmwd	40	8	this	this	DT
cord-104279-choywmwd	40	9	work	work	NN
cord-104279-choywmwd	40	10	are	be	VBP
cord-104279-choywmwd	40	11	shown	show	VBN
cord-104279-choywmwd	40	12	to	to	TO
cord-104279-choywmwd	40	13	be	be	VB
cord-104279-choywmwd	40	14	transported	transport	VBN
cord-104279-choywmwd	40	15	directly	directly	RB
cord-104279-choywmwd	40	16	to	to	IN
cord-104279-choywmwd	40	17	the	the	DT
cord-104279-choywmwd	40	18	vacuole	vacuole	NN
cord-104279-choywmwd	40	19	from	from	IN
cord-104279-choywmwd	40	20	the	the	DT
cord-104279-choywmwd	40	21	Golgi	Golgi	NNP
cord-104279-choywmwd	40	22	,	,	,
cord-104279-choywmwd	40	23	and	and	CC
cord-104279-choywmwd	40	24	not	not	RB
cord-104279-choywmwd	40	25	to	to	IN
cord-104279-choywmwd	40	26	the	the	DT
cord-104279-choywmwd	40	27	plasma	plasma	NN
cord-104279-choywmwd	40	28	membrane	membrane	NN
cord-104279-choywmwd	40	29	.	.	.
cord-104279-choywmwd	41	1	The	the	DT
cord-104279-choywmwd	41	2	bacterial	bacterial	JJ
cord-104279-choywmwd	41	3	strain	strain	NN
cord-104279-choywmwd	41	4	MC1061	mc1061	NN
cord-104279-choywmwd	41	5	(	(	-LRB-
cord-104279-choywmwd	41	6	Casadaban	Casadaban	NNP
cord-104279-choywmwd	41	7	and	and	CC
cord-104279-choywmwd	41	8	Cohen	Cohen	NNP
cord-104279-choywmwd	41	9	,	,	,
cord-104279-choywmwd	41	10	1980	1980	CD
cord-104279-choywmwd	41	11	)	)	-RRB-
cord-104279-choywmwd	41	12	was	be	VBD
cord-104279-choywmwd	41	13	used	use	VBN
cord-104279-choywmwd	41	14	for	for	IN
cord-104279-choywmwd	41	15	all	all	DT
cord-104279-choywmwd	41	16	subcloning	subclone	VBG
cord-104279-choywmwd	41	17	steps	step	NNS
cord-104279-choywmwd	41	18	.	.	.
cord-104279-choywmwd	42	1	Oligonucleotide	Oligonucleotide	NNP
cord-104279-choywmwd	42	2	-	-	HYPH
cord-104279-choywmwd	42	3	directed	direct	VBN
cord-104279-choywmwd	42	4	mutagenesis	mutagenesis	NN
cord-104279-choywmwd	42	5	was	be	VBD
cord-104279-choywmwd	42	6	carded	card	VBN
cord-104279-choywmwd	42	7	out	out	RP
cord-104279-choywmwd	42	8	in	in	IN
cord-104279-choywmwd	42	9	strain	strain	NN
cord-104279-choywmwd	43	1	CJ236	cj236	VB
cord-104279-choywmwd	44	1	(	(	-LRB-
cord-104279-choywmwd	44	2	Kunkel	Kunkel	NNP
cord-104279-choywmwd	44	3	et	et	NNP
cord-104279-choywmwd	44	4	al	al	NNP
cord-104279-choywmwd	44	5	.	.	NNP
cord-104279-choywmwd	44	6	,	,	,
cord-104279-choywmwd	44	7	1987	1987	CD
cord-104279-choywmwd	44	8	)	)	-RRB-
cord-104279-choywmwd	44	9	.	.	.
cord-104279-choywmwd	45	1	The	the	DT
cord-104279-choywmwd	45	2	yeast	yeast	NN
cord-104279-choywmwd	45	3	strains	strain	NNS
cord-104279-choywmwd	45	4	used	use	VBN
cord-104279-choywmwd	45	5	were	be	VBD
cord-104279-choywmwd	45	6	JHRY20	JHRY20	NNP
cord-104279-choywmwd	45	7	-	-	HYPH
cord-104279-choywmwd	45	8	1AAIA3	1AAIA3	NNP
cord-104279-choywmwd	45	9	(	(	-LRB-
cord-104279-choywmwd	45	10	MATa	MATa	NNS
cord-104279-choywmwd	45	11	,	,	,
cord-104279-choywmwd	45	12	dap2A	dap2A	NNP
cord-104279-choywmwd	45	13	:	:	:
cord-104279-choywmwd	45	14	:	:	:
cord-104279-choywmwd	45	15	H1S3	H1S3	NNP
cord-104279-choywmwd	45	16	,	,	,
cord-104279-choywmwd	45	17	stel3A	stel3a	NN
cord-104279-choywmwd	45	18	:	:	:
cord-104279-choywmwd	45	19	:	:	:
cord-104279-choywmwd	45	20	LEU2	LEU2	NNP
cord-104279-choywmwd	45	21	,	,	,
cord-104279-choywmwd	45	22	ura3	ura3	CD
cord-104279-choywmwd	45	23	-	-	SYM
cord-104279-choywmwd	45	24	52	52	CD
cord-104279-choywmwd	45	25	,	,	,
cord-104279-choywmwd	45	26	leu2	leu2	CD
cord-104279-choywmwd	45	27	-	-	SYM
cord-104279-choywmwd	45	28	3	3	CD
cord-104279-choywmwd	45	29	,	,	,
cord-104279-choywmwd	45	30	1eu2412	1eu2412	CD
cord-104279-choywmwd	45	31	,	,	,
cord-104279-choywmwd	45	32	his3-A200	his3-a200	NN
cord-104279-choywmwd	45	33	,	,	,
cord-104279-choywmwd	45	34	pep4	pep4	NN
cord-104279-choywmwd	45	35	-	-	HYPH
cord-104279-choywmwd	45	36	3	3	CD
cord-104279-choywmwd	45	37	;	;	:
cord-104279-choywmwd	45	38	 	 	_SP
cord-104279-choywmwd	45	39	Roberts	Roberts	NNP
cord-104279-choywmwd	45	40	et	et	NNP
cord-104279-choywmwd	45	41	al	al	NNP
cord-104279-choywmwd	45	42	.	.	NNP
cord-104279-choywmwd	45	43	,	,	,
cord-104279-choywmwd	45	44	1989	1989	CD
cord-104279-choywmwd	45	45	)	)	-RRB-
cord-104279-choywmwd	45	46	,	,	,
cord-104279-choywmwd	45	47	SEY2012zs	sey2012zs	NN
cord-104279-choywmwd	46	1	(	(	-LRB-
cord-104279-choywmwd	46	2	MATch	MATch	NNP
cord-104279-choywmwd	46	3	,	,	,
cord-104279-choywmwd	46	4	dap2	dap2	NN
cord-104279-choywmwd	46	5	:	:	:
cord-104279-choywmwd	46	6	:	:	:
cord-104279-choywmwd	46	7	LEU2	LEU2	NNP
cord-104279-choywmwd	46	8	,	,	,
cord-104279-choywmwd	46	9	 	 	_SP
cord-104279-choywmwd	46	10	Emr	Emr	NNP
cord-104279-choywmwd	46	11	et	et	NNP
cord-104279-choywmwd	46	12	al	al	NNP
cord-104279-choywmwd	46	13	.	.	NNP
cord-104279-choywmwd	46	14	,	,	,
cord-104279-choywmwd	46	15	1983	1983	CD
cord-104279-choywmwd	46	16	)	)	-RRB-
cord-104279-choywmwd	47	1	,	,	,
cord-104279-choywmwd	47	2	CJRY25	cjry25	NN
cord-104279-choywmwd	47	3	-	-	HYPH
cord-104279-choywmwd	47	4	6B	6b	JJ
cord-104279-choywmwd	47	5	(	(	-LRB-
cord-104279-choywmwd	47	6	MATa	MATa	NNP
cord-104279-choywmwd	47	7	dap2A	dap2A	NNP
cord-104279-choywmwd	47	8	:	:	:
cord-104279-choywmwd	47	9	:	:	:
cord-104279-choywmwd	47	10	LEU2	LEU2	NNP
cord-104279-choywmwd	47	11	mnn9	mnn9	CD
cord-104279-choywmwd	47	12	ura3	ura3	CD
cord-104279-choywmwd	47	13	-	-	SYM
cord-104279-choywmwd	47	14	52	52	CD
cord-104279-choywmwd	47	15	leu2	leu2	CD
cord-104279-choywmwd	47	16	-	-	HYPH
cord-104279-choywmwd	47	17	3	3	CD
cord-104279-choywmwd	47	18	leu2412	leu2412	NN
cord-104279-choywmwd	47	19	,	,	,
cord-104279-choywmwd	47	20	and	and	CC
cord-104279-choywmwd	47	21	SEY-5016	SEY-5016	NNP
cord-104279-choywmwd	47	22	(	(	-LRB-
cord-104279-choywmwd	47	23	MATa	MATa	NNS
cord-104279-choywmwd	47	24	,	,	,
cord-104279-choywmwd	47	25	s	s	NNP
cord-104279-choywmwd	47	26	e	e	NNP
cord-104279-choywmwd	47	27	cl	cl	NNP
cord-104279-choywmwd	47	28	-	-	HYPH
cord-104279-choywmwd	47	29	l	l	NNP
cord-104279-choywmwd	47	30	,	,	,
cord-104279-choywmwd	47	31	dap	dap	NNP
cord-104279-choywmwd	47	32	2	2	CD
cord-104279-choywmwd	47	33	:	:	:
cord-104279-choywmwd	47	34	:	:	:
cord-104279-choywmwd	48	1	LE	LE	NNP
cord-104279-choywmwd	48	2	U2	U2	NNP
cord-104279-choywmwd	48	3	,	,	,
cord-104279-choywmwd	48	4	ura3	ura3	NNP
cord-104279-choywmwd	48	5	-	-	SYM
cord-104279-choywmwd	48	6	5	5	CD
cord-104279-choywmwd	48	7	2	2	CD
cord-104279-choywmwd	48	8	,	,	,
cord-104279-choywmwd	48	9	le	le	NNP
cord-104279-choywmwd	48	10	u2	u2	NNP
cord-104279-choywmwd	48	11	-	-	HYPH
cord-104279-choywmwd	48	12	3	3	CD
cord-104279-choywmwd	48	13	,	,	,
cord-104279-choywmwd	48	14	leu2412	leu2412	NNP
cord-104279-choywmwd	48	15	)	)	-RRB-
cord-104279-choywmwd	48	16	.	.	.
cord-104279-choywmwd	49	1	Yeast	yeast	NN
cord-104279-choywmwd	49	2	cultures	culture	NNS
cord-104279-choywmwd	49	3	were	be	VBD
cord-104279-choywmwd	49	4	grown	grow	VBN
cord-104279-choywmwd	49	5	in	in	IN
cord-104279-choywmwd	49	6	YEPD	YEPD	NNP
cord-104279-choywmwd	49	7	or	or	CC
cord-104279-choywmwd	49	8	minimal	minimal	JJ
cord-104279-choywmwd	49	9	(	(	-LRB-
cord-104279-choywmwd	49	10	SD	sd	NN
cord-104279-choywmwd	49	11	)	)	-RRB-
cord-104279-choywmwd	49	12	medium	medium	NN
cord-104279-choywmwd	49	13	supplemented	supplement	VBN
cord-104279-choywmwd	49	14	with	with	IN
cord-104279-choywmwd	49	15	the	the	DT
cord-104279-choywmwd	49	16	appropriate	appropriate	JJ
cord-104279-choywmwd	49	17	nutrients	nutrient	NNS
cord-104279-choywmwd	49	18	as	as	IN
cord-104279-choywmwd	49	19	previously	previously	RB
cord-104279-choywmwd	49	20	described	describe	VBN
cord-104279-choywmwd	49	21	(	(	-LRB-
cord-104279-choywmwd	49	22	Sherman	Sherman	NNP
cord-104279-choywmwd	49	23	et	et	NNP
cord-104279-choywmwd	49	24	al	al	NNP
cord-104279-choywmwd	49	25	.	.	NNP
cord-104279-choywmwd	49	26	,	,	,
cord-104279-choywmwd	49	27	1982	1982	CD
cord-104279-choywmwd	49	28	)	)	-RRB-
cord-104279-choywmwd	49	29	.	.	.
cord-104279-choywmwd	50	1	For	for	IN
cord-104279-choywmwd	50	2	the	the	DT
cord-104279-choywmwd	50	3	simultaneous	simultaneous	JJ
cord-104279-choywmwd	50	4	induction	induction	NN
cord-104279-choywmwd	50	5	of	of	IN
cord-104279-choywmwd	50	6	the	the	DT
cord-104279-choywmwd	50	7	GAL/	gal/	JJ
cord-104279-choywmwd	50	8	promoter	promoter	NN
cord-104279-choywmwd	50	9	and	and	CC
cord-104279-choywmwd	50	10	the	the	DT
cord-104279-choywmwd	50	11	secl4	secl4	NN
cord-104279-choywmwd	50	12	secretion	secretion	NN
cord-104279-choywmwd	50	13	defect	defect	NN
cord-104279-choywmwd	50	14	,	,	,
cord-104279-choywmwd	50	15	ceils	ceil	NNS
cord-104279-choywmwd	50	16	were	be	VBD
cord-104279-choywmwd	50	17	grown	grow	VBN
cord-104279-choywmwd	50	18	to	to	TO
cord-104279-choywmwd	50	19	log	log	VB
cord-104279-choywmwd	50	20	phase	phase	NN
cord-104279-choywmwd	50	21	in	in	IN
cord-104279-choywmwd	50	22	minimal	minimal	JJ
cord-104279-choywmwd	50	23	media	medium	NNS
cord-104279-choywmwd	50	24	plus	plus	CC
cord-104279-choywmwd	50	25	raltinose	raltinose	NN
cord-104279-choywmwd	50	26	and	and	CC
cord-104279-choywmwd	50	27	then	then	RB
cord-104279-choywmwd	50	28	harvested	harvest	VBD
cord-104279-choywmwd	50	29	and	and	CC
cord-104279-choywmwd	50	30	resuspended	resuspend	VBD
cord-104279-choywmwd	50	31	in	in	IN
cord-104279-choywmwd	50	32	YEP	YEP	NNP
cord-104279-choywmwd	50	33	-	-	HYPH
cord-104279-choywmwd	50	34	raitinose	raitinose	NN
cord-104279-choywmwd	50	35	.	.	.
cord-104279-choywmwd	51	1	After	after	IN
cord-104279-choywmwd	51	2	a	a	DT
cord-104279-choywmwd	51	3	1-h	1-h	CD
cord-104279-choywmwd	51	4	incubation	incubation	NN
cord-104279-choywmwd	51	5	at	at	IN
cord-104279-choywmwd	51	6	25~	25~	CD
cord-104279-choywmwd	51	7	galactose	galactose	NN
cord-104279-choywmwd	51	8	was	be	VBD
cord-104279-choywmwd	51	9	added	add	VBN
cord-104279-choywmwd	51	10	directly	directly	RB
cord-104279-choywmwd	51	11	to	to	IN
cord-104279-choywmwd	51	12	the	the	DT
cord-104279-choywmwd	51	13	cultures	culture	NNS
cord-104279-choywmwd	51	14	and	and	CC
cord-104279-choywmwd	51	15	at	at	IN
cord-104279-choywmwd	51	16	the	the	DT
cord-104279-choywmwd	51	17	same	same	JJ
cord-104279-choywmwd	51	18	time	time	NN
cord-104279-choywmwd	51	19	the	the	DT
cord-104279-choywmwd	51	20	cultures	culture	NNS
cord-104279-choywmwd	51	21	were	be	VBD
cord-104279-choywmwd	51	22	shifted	shift	VBN
cord-104279-choywmwd	51	23	to	to	IN
cord-104279-choywmwd	51	24	34~	34~	CD
cord-104279-choywmwd	51	25	After	after	IN
cord-104279-choywmwd	51	26	2	2	CD
cord-104279-choywmwd	51	27	h	h	NN
cord-104279-choywmwd	51	28	,	,	,
cord-104279-choywmwd	51	29	the	the	DT
cord-104279-choywmwd	51	30	cells	cell	NNS
cord-104279-choywmwd	51	31	were	be	VBD
cord-104279-choywmwd	51	32	fixed	fix	VBN
cord-104279-choywmwd	51	33	immediately	immediately	RB
cord-104279-choywmwd	51	34	and	and	CC
cord-104279-choywmwd	51	35	prepared	prepare	VBN
cord-104279-choywmwd	51	36	for	for	IN
cord-104279-choywmwd	51	37	indirect	indirect	JJ
cord-104279-choywmwd	51	38	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	51	39	as	as	IN
cord-104279-choywmwd	51	40	described	describe	VBN
cord-104279-choywmwd	51	41	below	below	RB
cord-104279-choywmwd	51	42	.	.	.
cord-104279-choywmwd	52	1	Oligonucleotides	Oligonucleotides	NNP
cord-104279-choywmwd	52	2	for	for	IN
cord-104279-choywmwd	52	3	mutagenesis	mutagenesis	NN
cord-104279-choywmwd	52	4	were	be	VBD
cord-104279-choywmwd	52	5	prepared	prepare	VBN
cord-104279-choywmwd	52	6	by	by	IN
cord-104279-choywmwd	52	7	the	the	DT
cord-104279-choywmwd	52	8	University	University	NNP
cord-104279-choywmwd	52	9	of	of	IN
cord-104279-choywmwd	52	10	Oregon	Oregon	NNP
cord-104279-choywmwd	52	11	Biotechnology	Biotechnology	NNP
cord-104279-choywmwd	52	12	Laboratory	Laboratory	NNP
cord-104279-choywmwd	52	13	on	on	IN
cord-104279-choywmwd	52	14	an	an	DT
cord-104279-choywmwd	52	15	Applied	Applied	NNP
cord-104279-choywmwd	52	16	Biosystems	Biosystems	NNP
cord-104279-choywmwd	52	17	380B	380b	CD
cord-104279-choywmwd	52	18	DNA	dna	NN
cord-104279-choywmwd	52	19	synthesizer	synthesizer	NN
cord-104279-choywmwd	52	20	(	(	-LRB-
cord-104279-choywmwd	52	21	Foster	Foster	NNP
cord-104279-choywmwd	52	22	City	City	NNP
cord-104279-choywmwd	52	23	,	,	,
cord-104279-choywmwd	52	24	CA	CA	NNP
cord-104279-choywmwd	52	25	)	)	-RRB-
cord-104279-choywmwd	52	26	as	as	IN
cord-104279-choywmwd	52	27	described	describe	VBN
cord-104279-choywmwd	52	28	(	(	-LRB-
cord-104279-choywmwd	52	29	Ito	Ito	NNP
cord-104279-choywmwd	52	30	et	et	NNP
cord-104279-choywmwd	52	31	al	al	NNP
cord-104279-choywmwd	52	32	.	.	NNP
cord-104279-choywmwd	52	33	,	,	,
cord-104279-choywmwd	52	34	1982	1982	CD
cord-104279-choywmwd	52	35	)	)	-RRB-
cord-104279-choywmwd	52	36	.	.	.
cord-104279-choywmwd	53	1	Tran35S	Tran35S	NNP
cord-104279-choywmwd	53	2	-	-	HYPH
cord-104279-choywmwd	53	3	label	label	NNP
cord-104279-choywmwd	53	4	and	and	CC
cord-104279-choywmwd	53	5	Zymolyase	zymolyase	NN
cord-104279-choywmwd	54	1	100	100	CD
cord-104279-choywmwd	54	2	T	T	NNS
cord-104279-choywmwd	54	3	were	be	VBD
cord-104279-choywmwd	54	4	from	from	IN
cord-104279-choywmwd	54	5	ICN	ICN	NNP
cord-104279-choywmwd	54	6	Biomedicals	Biomedicals	NNP
cord-104279-choywmwd	54	7	(	(	-LRB-
cord-104279-choywmwd	54	8	Irvine	Irvine	NNP
cord-104279-choywmwd	54	9	,	,	,
cord-104279-choywmwd	54	10	CA	CA	NNP
cord-104279-choywmwd	54	11	)	)	-RRB-
cord-104279-choywmwd	54	12	,	,	,
cord-104279-choywmwd	54	13	Endo	Endo	NNP
cord-104279-choywmwd	54	14	H	H	NNP
cord-104279-choywmwd	54	15	was	be	VBD
cord-104279-choywmwd	54	16	from	from	IN
cord-104279-choywmwd	54	17	Boehringer	Boehringer	NNP
cord-104279-choywmwd	54	18	Marmheim	Marmheim	NNP
cord-104279-choywmwd	54	19	(	(	-LRB-
cord-104279-choywmwd	54	20	Indianapolis	Indianapolis	NNP
cord-104279-choywmwd	54	21	,	,	,
cord-104279-choywmwd	54	22	IN	in	IN
cord-104279-choywmwd	54	23	)	)	-RRB-
cord-104279-choywmwd	54	24	,	,	,
cord-104279-choywmwd	54	25	ultra	ultra	JJ
cord-104279-choywmwd	54	26	-	-	JJ
cord-104279-choywmwd	54	27	pure	pure	JJ
cord-104279-choywmwd	54	28	SDS	SDS	NNP
cord-104279-choywmwd	54	29	was	be	VBD
cord-104279-choywmwd	54	30	from	from	IN
cord-104279-choywmwd	54	31	BDH	BDH	NNP
cord-104279-choywmwd	54	32	Biochemicals	Biochemicals	NNPS
cord-104279-choywmwd	54	33	(	(	-LRB-
cord-104279-choywmwd	54	34	San	San	NNP
cord-104279-choywmwd	54	35	Francisco	Francisco	NNP
cord-104279-choywmwd	54	36	,	,	,
cord-104279-choywmwd	54	37	CA	CA	NNP
cord-104279-choywmwd	54	38	)	)	-RRB-
cord-104279-choywmwd	54	39	,	,	,
cord-104279-choywmwd	54	40	glusulase	glusulase	NNP
cord-104279-choywmwd	54	41	was	be	VBD
cord-104279-choywmwd	54	42	from	from	IN
cord-104279-choywmwd	54	43	Dupont	Dupont	NNP
cord-104279-choywmwd	54	44	Pharmaceuticals	Pharmaceuticals	NNP
cord-104279-choywmwd	54	45	(	(	-LRB-
cord-104279-choywmwd	54	46	Wilmington	Wilmington	NNP
cord-104279-choywmwd	54	47	,	,	,
cord-104279-choywmwd	54	48	DE	DE	NNP
cord-104279-choywmwd	54	49	)	)	-RRB-
cord-104279-choywmwd	54	50	,	,	,
cord-104279-choywmwd	54	51	and	and	CC
cord-104279-choywmwd	54	52	all	all	DT
cord-104279-choywmwd	54	53	antibodies	antibody	NNS
cord-104279-choywmwd	54	54	(	(	-LRB-
cord-104279-choywmwd	54	55	except	except	IN
cord-104279-choywmwd	54	56	anti	anti	JJ
cord-104279-choywmwd	54	57	-	-	JJ
cord-104279-choywmwd	54	58	DPAP	DPAP	NNP
cord-104279-choywmwd	54	59	B	b	NN
cord-104279-choywmwd	54	60	,	,	,
cord-104279-choywmwd	54	61	anti	anti	JJ
cord-104279-choywmwd	54	62	-	-	JJ
cord-104279-choywmwd	54	63	DPAP	DPAP	NNP
cord-104279-choywmwd	54	64	A	A	NNP
cord-104279-choywmwd	54	65	,	,	,
cord-104279-choywmwd	54	66	anti	anti	JJ
cord-104279-choywmwd	54	67	-	-	JJ
cord-104279-choywmwd	54	68	ALP	ALP	NNP
cord-104279-choywmwd	54	69	,	,	,
cord-104279-choywmwd	54	70	and	and	CC
cord-104279-choywmwd	54	71	anti	anti	JJ
cord-104279-choywmwd	54	72	-	-	JJ
cord-104279-choywmwd	54	73	Vat2p	vat2p	JJ
cord-104279-choywmwd	54	74	antibodies	antibody	NNS
cord-104279-choywmwd	54	75	)	)	-RRB-
cord-104279-choywmwd	54	76	used	use	VBN
cord-104279-choywmwd	54	77	for	for	IN
cord-104279-choywmwd	54	78	indirect	indirect	JJ
cord-104279-choywmwd	54	79	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	54	80	were	be	VBD
cord-104279-choywmwd	54	81	from	from	IN
cord-104279-choywmwd	54	82	Jackson	Jackson	NNP
cord-104279-choywmwd	54	83	ImmunoResearch	ImmunoResearch	NNP
cord-104279-choywmwd	54	84	(	(	-LRB-
cord-104279-choywmwd	54	85	West	West	NNP
cord-104279-choywmwd	54	86	Grove	Grove	NNP
cord-104279-choywmwd	54	87	,	,	,
cord-104279-choywmwd	54	88	PA	PA	NNP
cord-104279-choywmwd	54	89	)	)	-RRB-
cord-104279-choywmwd	54	90	,	,	,
cord-104279-choywmwd	54	91	Cappel	Cappel	NNP
cord-104279-choywmwd	54	92	Products	Products	NNP
cord-104279-choywmwd	54	93	(	(	-LRB-
cord-104279-choywmwd	54	94	Malvern	Malvern	NNP
cord-104279-choywmwd	54	95	,	,	,
cord-104279-choywmwd	54	96	PA	PA	NNP
cord-104279-choywmwd	54	97	)	)	-RRB-
cord-104279-choywmwd	54	98	,	,	,
cord-104279-choywmwd	54	99	or	or	CC
cord-104279-choywmwd	54	100	Promega	Promega	NNP
cord-104279-choywmwd	54	101	Biotech	Biotech	NNP
cord-104279-choywmwd	54	102	(	(	-LRB-
cord-104279-choywmwd	54	103	Madison	Madison	NNP
cord-104279-choywmwd	54	104	,	,	,
cord-104279-choywmwd	54	105	WI	WI	NNP
cord-104279-choywmwd	54	106	)	)	-RRB-
cord-104279-choywmwd	54	107	.	.	.
cord-104279-choywmwd	55	1	All	all	DT
cord-104279-choywmwd	55	2	other	other	JJ
cord-104279-choywmwd	55	3	reagents	reagent	NNS
cord-104279-choywmwd	55	4	were	be	VBD
cord-104279-choywmwd	55	5	from	from	IN
cord-104279-choywmwd	55	6	Sigma	Sigma	NNP
cord-104279-choywmwd	55	7	Chemical	Chemical	NNP
cord-104279-choywmwd	55	8	Co.	Co.	NNP
cord-104279-choywmwd	55	9	(	(	-LRB-
cord-104279-choywmwd	55	10	St.	St.	NNP
cord-104279-choywmwd	55	11	Louis	Louis	NNP
cord-104279-choywmwd	55	12	,	,	,
cord-104279-choywmwd	55	13	MO	MO	NNP
cord-104279-choywmwd	55	14	)	)	-RRB-
cord-104279-choywmwd	55	15	.	.	.
cord-104279-choywmwd	56	1	DPAP	DPAP	NNP
cord-104279-choywmwd	56	2	and	and	CC
cord-104279-choywmwd	56	3	invertase	invertase	NNP
cord-104279-choywmwd	56	4	assays	assay	NNS
cord-104279-choywmwd	56	5	were	be	VBD
cord-104279-choywmwd	56	6	performed	perform	VBN
cord-104279-choywmwd	56	7	as	as	IN
cord-104279-choywmwd	56	8	previously	previously	RB
cord-104279-choywmwd	56	9	described	describe	VBN
cord-104279-choywmwd	56	10	(	(	-LRB-
cord-104279-choywmwd	56	11	Gildstein	Gildstein	NNP
cord-104279-choywmwd	56	12	and	and	CC
cord-104279-choywmwd	56	13	Lampen	Lampen	NNP
cord-104279-choywmwd	56	14	,	,	,
cord-104279-choywmwd	56	15	1975	1975	CD
cord-104279-choywmwd	56	16	;	;	:
cord-104279-choywmwd	56	17	Roberts	Roberts	NNP
cord-104279-choywmwd	56	18	et	et	NNP
cord-104279-choywmwd	56	19	al	al	NNP
cord-104279-choywmwd	56	20	.	.	NNP
cord-104279-choywmwd	56	21	,	,	,
cord-104279-choywmwd	56	22	1991	1991	CD
cord-104279-choywmwd	56	23	)	)	-RRB-
cord-104279-choywmwd	56	24	.	.	.
cord-104279-choywmwd	57	1	Restriction	restriction	NN
cord-104279-choywmwd	57	2	endonuclease	endonuclease	NN
cord-104279-choywmwd	57	3	digests	digest	VBZ
cord-104279-choywmwd	57	4	and	and	CC
cord-104279-choywmwd	57	5	ligations	ligation	NNS
cord-104279-choywmwd	57	6	were	be	VBD
cord-104279-choywmwd	57	7	performed	perform	VBN
cord-104279-choywmwd	57	8	as	as	IN
cord-104279-choywmwd	57	9	recommended	recommend	VBN
cord-104279-choywmwd	57	10	by	by	IN
cord-104279-choywmwd	57	11	the	the	DT
cord-104279-choywmwd	57	12	suppliers	supplier	NNS
cord-104279-choywmwd	57	13	.	.	.
cord-104279-choywmwd	58	1	Plasmid	plasmid	NN
cord-104279-choywmwd	58	2	purification	purification	NN
cord-104279-choywmwd	58	3	,	,	,
cord-104279-choywmwd	58	4	agarose	agarose	JJ
cord-104279-choywmwd	58	5	gel	gel	NN
cord-104279-choywmwd	58	6	electrophoresis	electrophoresis	NN
cord-104279-choywmwd	58	7	,	,	,
cord-104279-choywmwd	58	8	fill	fill	VB
cord-104279-choywmwd	58	9	-	-	HYPH
cord-104279-choywmwd	58	10	in	in	IN
cord-104279-choywmwd	58	11	reactions	reaction	NNS
cord-104279-choywmwd	58	12	of	of	IN
cord-104279-choywmwd	58	13	sticky	sticky	RB
cord-104279-choywmwd	58	14	-	-	HYPH
cord-104279-choywmwd	58	15	ended	ended	JJ
cord-104279-choywmwd	58	16	DNA	dna	NN
cord-104279-choywmwd	58	17	fragments	fragment	NNS
cord-104279-choywmwd	58	18	using	use	VBG
cord-104279-choywmwd	58	19	T4	T4	NNP
cord-104279-choywmwd	58	20	DNA	DNA	NNP
cord-104279-choywmwd	58	21	polymerase	polymerase	NN
cord-104279-choywmwd	58	22	,	,	,
cord-104279-choywmwd	58	23	and	and	CC
cord-104279-choywmwd	58	24	DNA	dna	NN
cord-104279-choywmwd	58	25	-	-	HYPH
cord-104279-choywmwd	58	26	mediated	mediate	VBN
cord-104279-choywmwd	58	27	transformation	transformation	NN
cord-104279-choywmwd	58	28	of	of	IN
cord-104279-choywmwd	58	29	Escherichia	Escherichia	NNP
cord-104279-choywmwd	58	30	coli	coli	NNS
cord-104279-choywmwd	58	31	were	be	VBD
cord-104279-choywmwd	58	32	done	do	VBN
cord-104279-choywmwd	58	33	according	accord	VBG
cord-104279-choywmwd	58	34	to	to	IN
cord-104279-choywmwd	58	35	standard	standard	JJ
cord-104279-choywmwd	58	36	procedures	procedure	NNS
cord-104279-choywmwd	58	37	(	(	-LRB-
cord-104279-choywmwd	58	38	Maniatis	Maniatis	NNP
cord-104279-choywmwd	58	39	et	et	NNP
cord-104279-choywmwd	58	40	al	al	NNP
cord-104279-choywmwd	58	41	.	.	NNP
cord-104279-choywmwd	58	42	,	,	,
cord-104279-choywmwd	58	43	1982	1982	CD
cord-104279-choywmwd	58	44	)	)	-RRB-
cord-104279-choywmwd	58	45	.	.	.
cord-104279-choywmwd	59	1	Lithium	lithium	NN
cord-104279-choywmwd	59	2	acetate	acetate	NN
cord-104279-choywmwd	59	3	transformations	transformation	NNS
cord-104279-choywmwd	59	4	of	of	IN
cord-104279-choywmwd	59	5	yeast	yeast	NN
cord-104279-choywmwd	59	6	were	be	VBD
cord-104279-choywmwd	59	7	performed	perform	VBN
cord-104279-choywmwd	59	8	as	as	IN
cord-104279-choywmwd	59	9	described	describe	VBN
cord-104279-choywmwd	59	10	(	(	-LRB-
cord-104279-choywmwd	59	11	Jto	Jto	NNP
cord-104279-choywmwd	59	12	et	et	NNP
cord-104279-choywmwd	59	13	al	al	NNP
cord-104279-choywmwd	59	14	.	.	NNP
cord-104279-choywmwd	59	15	,	,	,
cord-104279-choywmwd	59	16	1983	1983	CD
cord-104279-choywmwd	59	17	)	)	-RRB-
cord-104279-choywmwd	59	18	.	.	.
cord-104279-choywmwd	60	1	A	a	DT
cord-104279-choywmwd	60	2	disruption	disruption	NN
cord-104279-choywmwd	60	3	of	of	IN
cord-104279-choywmwd	60	4	the	the	DT
cord-104279-choywmwd	60	5	chromosomal	chromosomal	JJ
cord-104279-choywmwd	60	6	STE/3	STE/3	NNP
cord-104279-choywmwd	60	7	locus	locus	NN
cord-104279-choywmwd	60	8	was	be	VBD
cord-104279-choywmwd	60	9	constructed	construct	VBN
cord-104279-choywmwd	60	10	by	by	IN
cord-104279-choywmwd	60	11	onestep	onestep	NN
cord-104279-choywmwd	60	12	gene	gene	NN
cord-104279-choywmwd	60	13	disruption	disruption	NN
cord-104279-choywmwd	60	14	(	(	-LRB-
cord-104279-choywmwd	60	15	l	l	NNP
cord-104279-choywmwd	60	16	~	~	CD
cord-104279-choywmwd	60	17	thstein	thstein	NNP
cord-104279-choywmwd	60	18	,	,	,
cord-104279-choywmwd	60	19	1983	1983	CD
cord-104279-choywmwd	60	20	)	)	-RRB-
cord-104279-choywmwd	60	21	,	,	,
cord-104279-choywmwd	60	22	using	use	VBG
cord-104279-choywmwd	60	23	the	the	DT
cord-104279-choywmwd	60	24	plasmid	plasmid	NN
cord-104279-choywmwd	60	25	pSLK349	pSLK349	NNP
cord-104279-choywmwd	60	26	(	(	-LRB-
cord-104279-choywmwd	60	27	kindly	kindly	RB
cord-104279-choywmwd	60	28	provided	provide	VBN
cord-104279-choywmwd	60	29	by	by	IN
cord-104279-choywmwd	60	30	Dr.	Dr.	NNP
cord-104279-choywmwd	60	31	George	George	NNP
cord-104279-choywmwd	60	32	Sprague	Sprague	NNP
cord-104279-choywmwd	60	33	)	)	-RRB-
cord-104279-choywmwd	60	34	.	.	.
cord-104279-choywmwd	61	1	pSL349	pSL349	NNP
cord-104279-choywmwd	61	2	consists	consist	VBZ
cord-104279-choywmwd	61	3	of	of	IN
cord-104279-choywmwd	61	4	pBR322	pbr322	LS
cord-104279-choywmwd	61	5	containing	contain	VBG
cord-104279-choywmwd	61	6	a	a	DT
cord-104279-choywmwd	61	7	7.2	7.2	CD
cord-104279-choywmwd	61	8	kbp	kbp	NNP
cord-104279-choywmwd	61	9	BamHI	BamHI	NNP
cord-104279-choywmwd	61	10	STE/3	STE/3	NNP
cord-104279-choywmwd	61	11	fragment	fragment	NN
cord-104279-choywmwd	61	12	,	,	,
cord-104279-choywmwd	61	13	from	from	IN
cord-104279-choywmwd	61	14	which	which	WDT
cord-104279-choywmwd	61	15	a	a	DT
cord-104279-choywmwd	61	16	1.6-kbp	1.6-kbp	CD
cord-104279-choywmwd	61	17	BclI	BclI	NNP
cord-104279-choywmwd	61	18	fragment	fragment	NN
cord-104279-choywmwd	61	19	within	within	IN
cord-104279-choywmwd	61	20	the	the	DT
cord-104279-choywmwd	61	21	coding	code	VBG
cord-104279-choywmwd	61	22	region	region	NN
cord-104279-choywmwd	61	23	of	of	IN
cord-104279-choywmwd	61	24	the	the	DT
cord-104279-choywmwd	61	25	DPAP	DPAP	NNP
cord-104279-choywmwd	61	26	A	a	DT
cord-104279-choywmwd	61	27	lumenal	lumenal	JJ
cord-104279-choywmwd	61	28	domain	domain	NN
cord-104279-choywmwd	61	29	(	(	-LRB-
cord-104279-choywmwd	61	30	C.	C.	NNP
cord-104279-choywmwd	61	31	A.	A.	NNP
cord-104279-choywmwd	61	32	Flanagan	Flanagan	NNP
cord-104279-choywmwd	61	33	,	,	,
cord-104279-choywmwd	61	34	D.	D.	NNP
cord-104279-choywmwd	61	35	A.	A.	NNP
cord-104279-choywmwd	61	36	Barnes	Barnes	NNP
cord-104279-choywmwd	61	37	,	,	,
cord-104279-choywmwd	61	38	M.	M.	NNP
cord-104279-choywmwd	61	39	C.	C.	NNP
cord-104279-choywmwd	61	40	Flessel	Flessel	NNP
cord-104279-choywmwd	61	41	,	,	,
cord-104279-choywmwd	61	42	and	and	CC
cord-104279-choywmwd	61	43	J.	J.	NNP
cord-104279-choywmwd	61	44	Thorner	Thorner	NNP
cord-104279-choywmwd	61	45	,	,	,
cord-104279-choywmwd	61	46	manuscript	manuscript	NN
cord-104279-choywmwd	61	47	submitted	submit	VBN
cord-104279-choywmwd	61	48	for	for	IN
cord-104279-choywmwd	61	49	publication	publication	NN
cord-104279-choywmwd	61	50	)	)	-RRB-
cord-104279-choywmwd	61	51	was	be	VBD
cord-104279-choywmwd	61	52	replaced	replace	VBN
cord-104279-choywmwd	61	53	by	by	IN
cord-104279-choywmwd	61	54	the	the	DT
cord-104279-choywmwd	61	55	2.9-kbp	2.9-kbp	CD
cord-104279-choywmwd	61	56	BglII	BglII	NNP
cord-104279-choywmwd	61	57	LEU2	LEU2	NNP
cord-104279-choywmwd	61	58	fragment	fragment	NN
cord-104279-choywmwd	61	59	.	.	.
cord-104279-choywmwd	62	1	To	to	TO
cord-104279-choywmwd	62	2	create	create	VB
cord-104279-choywmwd	62	3	a	a	DT
cord-104279-choywmwd	62	4	strain	strain	NN
cord-104279-choywmwd	62	5	lacking	lack	VBG
cord-104279-choywmwd	62	6	both	both	DT
cord-104279-choywmwd	62	7	DPAP	DPAP	NNP
cord-104279-choywmwd	62	8	A	A	NNP
cord-104279-choywmwd	62	9	and	and	CC
cord-104279-choywmwd	62	10	DPAP	DPAP	NNP
cord-104279-choywmwd	62	11	B	B	NNP
cord-104279-choywmwd	62	12	,	,	,
cord-104279-choywmwd	62	13	a	a	DT
cord-104279-choywmwd	62	14	disruption	disruption	NN
cord-104279-choywmwd	62	15	of	of	IN
cord-104279-choywmwd	62	16	the	the	DT
cord-104279-choywmwd	62	17	chromosomal	chromosomal	JJ
cord-104279-choywmwd	62	18	DAP2	DAP2	NNP
cord-104279-choywmwd	62	19	locus	locus	NN
cord-104279-choywmwd	62	20	with	with	IN
cord-104279-choywmwd	62	21	the	the	DT
cord-104279-choywmwd	62	22	HIS3	HIS3	NNP
cord-104279-choywmwd	62	23	gene	gene	NN
cord-104279-choywmwd	62	24	was	be	VBD
cord-104279-choywmwd	62	25	made	make	VBN
cord-104279-choywmwd	62	26	using	use	VBG
cord-104279-choywmwd	62	27	the	the	DT
cord-104279-choywmwd	62	28	plasmid	plasmid	NN
cord-104279-choywmwd	62	29	pGP6	pGP6	NNP
cord-104279-choywmwd	62	30	,	,	,
cord-104279-choywmwd	62	31	which	which	WDT
cord-104279-choywmwd	62	32	contains	contain	VBZ
cord-104279-choywmwd	62	33	the	the	DT
cord-104279-choywmwd	62	34	1.2-kbp	1.2-kbp	CD
cord-104279-choywmwd	62	35	EcoRI	ecori	NN
cord-104279-choywmwd	62	36	-	-	HYPH
cord-104279-choywmwd	62	37	BamHI	BamHI	NNP
cord-104279-choywmwd	62	38	HIS3	HIS3	NNP
cord-104279-choywmwd	62	39	fragment	fragment	NN
cord-104279-choywmwd	62	40	(	(	-LRB-
cord-104279-choywmwd	62	41	Sikorski	Sikorski	NNP
cord-104279-choywmwd	62	42	and	and	CC
cord-104279-choywmwd	62	43	Hieter	Hieter	NNP
cord-104279-choywmwd	62	44	,	,	,
cord-104279-choywmwd	62	45	1989	1989	CD
cord-104279-choywmwd	62	46	)	)	-RRB-
cord-104279-choywmwd	62	47	in	in	IN
cord-104279-choywmwd	62	48	place	place	NN
cord-104279-choywmwd	62	49	of	of	IN
cord-104279-choywmwd	62	50	the	the	DT
cord-104279-choywmwd	62	51	L3-kbp	L3-kbp	NNP
cord-104279-choywmwd	62	52	BstEII	BstEII	NNP
cord-104279-choywmwd	62	53	-	-	HYPH
cord-104279-choywmwd	62	54	KpnI	KpnI	NNP
cord-104279-choywmwd	62	55	portion	portion	NN
cord-104279-choywmwd	62	56	of	of	IN
cord-104279-choywmwd	62	57	the	the	DT
cord-104279-choywmwd	62	58	coding	code	VBG
cord-104279-choywmwd	62	59	region	region	NN
cord-104279-choywmwd	62	60	of	of	IN
cord-104279-choywmwd	62	61	DAP2	DAP2	NNP
cord-104279-choywmwd	63	1	(	(	-LRB-
cord-104279-choywmwd	63	2	Roberts	Roberts	NNP
cord-104279-choywmwd	63	3	et	et	NNP
cord-104279-choywmwd	63	4	al	al	NNP
cord-104279-choywmwd	63	5	.	.	NNP
cord-104279-choywmwd	63	6	,	,	,
cord-104279-choywmwd	63	7	1989	1989	CD
cord-104279-choywmwd	63	8	)	)	-RRB-
cord-104279-choywmwd	63	9	.	.	.
cord-104279-choywmwd	64	1	The	the	DT
cord-104279-choywmwd	64	2	c~-factor	c~-factor	NN
cord-104279-choywmwd	64	3	signal	signal	NN
cord-104279-choywmwd	64	4	sequence	sequence	NN
cord-104279-choywmwd	64	5	was	be	VBD
cord-104279-choywmwd	64	6	fused	fuse	VBN
cord-104279-choywmwd	64	7	to	to	IN
cord-104279-choywmwd	64	8	the	the	DT
cord-104279-choywmwd	64	9	lumenal	lumenal	JJ
cord-104279-choywmwd	64	10	domain	domain	NN
cord-104279-choywmwd	64	11	of	of	IN
cord-104279-choywmwd	64	12	DPAP	DPAP	NNP
cord-104279-choywmwd	64	13	B	B	NNP
cord-104279-choywmwd	64	14	as	as	IN
cord-104279-choywmwd	64	15	follows	follow	VBZ
cord-104279-choywmwd	64	16	:	:	:
cord-104279-choywmwd	64	17	a	a	DT
cord-104279-choywmwd	64	18	Sail	Sail	NNP
cord-104279-choywmwd	64	19	linker	linker	NN
cord-104279-choywmwd	64	20	was	be	VBD
cord-104279-choywmwd	64	21	inserted	insert	VBN
cord-104279-choywmwd	64	22	at	at	IN
cord-104279-choywmwd	64	23	the	the	DT
cord-104279-choywmwd	64	24	Hincll	Hincll	NNP
cord-104279-choywmwd	64	25	site	site	NN
cord-104279-choywmwd	64	26	of	of	IN
cord-104279-choywmwd	64	27	plasmid	plasmid	NN
cord-104279-choywmwd	64	28	p771	p771	NN
cord-104279-choywmwd	64	29	,	,	,
cord-104279-choywmwd	64	30	which	which	WDT
cord-104279-choywmwd	64	31	contains	contain	VBZ
cord-104279-choywmwd	64	32	a	a	DT
cord-104279-choywmwd	64	33	portion	portion	NN
cord-104279-choywmwd	64	34	of	of	IN
cord-104279-choywmwd	64	35	the	the	DT
cord-104279-choywmwd	64	36	5	5	CD
cord-104279-choywmwd	64	37	'	'	POS
cord-104279-choywmwd	64	38	region	region	NN
cord-104279-choywmwd	64	39	of	of	IN
cord-104279-choywmwd	64	40	the	the	DT
cord-104279-choywmwd	64	41	MFcd	MFcd	NNP
cord-104279-choywmwd	64	42	gene	gene	NN
cord-104279-choywmwd	64	43	(	(	-LRB-
cord-104279-choywmwd	64	44	Kurjan	Kurjan	NNP
cord-104279-choywmwd	64	45	and	and	CC
cord-104279-choywmwd	64	46	Herskowitz	Herskowitz	NNP
cord-104279-choywmwd	64	47	,	,	,
cord-104279-choywmwd	64	48	1982	1982	CD
cord-104279-choywmwd	64	49	)	)	-RRB-
cord-104279-choywmwd	64	50	,	,	,
cord-104279-choywmwd	64	51	including	include	VBG
cord-104279-choywmwd	64	52	70	70	CD
cord-104279-choywmwd	64	53	bp	bp	NNS
cord-104279-choywmwd	64	54	of	of	IN
cord-104279-choywmwd	64	55	non	non	JJ
cord-104279-choywmwd	64	56	-	-	JJ
cord-104279-choywmwd	64	57	coding	code	VBG
cord-104279-choywmwd	64	58	region	region	NN
cord-104279-choywmwd	64	59	(	(	-LRB-
cord-104279-choywmwd	64	60	not	not	RB
cord-104279-choywmwd	64	61	including	include	VBG
cord-104279-choywmwd	64	62	the	the	DT
cord-104279-choywmwd	64	63	UAS	UAS	NNP
cord-104279-choywmwd	64	64	)	)	-RRB-
cord-104279-choywmwd	64	65	and	and	CC
cord-104279-choywmwd	64	66	66	66	CD
cord-104279-choywmwd	64	67	bp	bp	NN
cord-104279-choywmwd	64	68	of	of	IN
cord-104279-choywmwd	64	69	coding	code	VBG
cord-104279-choywmwd	64	70	region	region	NN
cord-104279-choywmwd	64	71	,	,	,
cord-104279-choywmwd	64	72	including	include	VBG
cord-104279-choywmwd	64	73	the	the	DT
cord-104279-choywmwd	64	74	signal	signal	NN
cord-104279-choywmwd	64	75	sequence	sequence	NN
cord-104279-choywmwd	64	76	,	,	,
cord-104279-choywmwd	64	77	signal	signal	NN
cord-104279-choywmwd	64	78	peptidase	peptidase	NN
cord-104279-choywmwd	64	79	cleavage	cleavage	NN
cord-104279-choywmwd	64	80	site	site	NN
cord-104279-choywmwd	64	81	(	(	-LRB-
cord-104279-choywmwd	64	82	Waters	Waters	NNP
cord-104279-choywmwd	64	83	et	et	NNP
cord-104279-choywmwd	64	84	al	al	NNP
cord-104279-choywmwd	64	85	.	.	NNP
cord-104279-choywmwd	64	86	,	,	,
cord-104279-choywmwd	64	87	1988	1988	CD
cord-104279-choywmwd	64	88	)	)	-RRB-
cord-104279-choywmwd	64	89	,	,	,
cord-104279-choywmwd	64	90	and	and	CC
cord-104279-choywmwd	64	91	3	3	CD
cord-104279-choywmwd	64	92	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	64	93	residues	residue	NNS
cord-104279-choywmwd	64	94	of	of	IN
cord-104279-choywmwd	64	95	pro	pro	JJ
cord-104279-choywmwd	64	96	-	-	JJ
cord-104279-choywmwd	64	97	a	a	DT
cord-104279-choywmwd	64	98	-	-	HYPH
cord-104279-choywmwd	64	99	factor	factor	NN
cord-104279-choywmwd	64	100	,	,	,
cord-104279-choywmwd	64	101	fused	fuse	VBN
cord-104279-choywmwd	64	102	to	to	IN
cord-104279-choywmwd	64	103	the	the	DT
cord-104279-choywmwd	64	104	KRE	KRE	NNP
cord-104279-choywmwd	64	105	/	/	SYM
cord-104279-choywmwd	64	106	gene	gene	NN
cord-104279-choywmwd	65	1	(	(	-LRB-
cord-104279-choywmwd	65	2	Boone	Boone	NNP
cord-104279-choywmwd	65	3	et	et	NNP
cord-104279-choywmwd	65	4	ai	ai	NNP
cord-104279-choywmwd	65	5	.	.	NNP
cord-104279-choywmwd	65	6	,	,	,
cord-104279-choywmwd	65	7	1990	1990	CD
cord-104279-choywmwd	65	8	)	)	-RRB-
cord-104279-choywmwd	65	9	.	.	.
cord-104279-choywmwd	66	1	The	the	DT
cord-104279-choywmwd	66	2	AccI	AccI	NNP
cord-104279-choywmwd	66	3	site	site	NN
cord-104279-choywmwd	66	4	at	at	IN
cord-104279-choywmwd	66	5	position	position	NN
cord-104279-choywmwd	66	6	+140	+140	NNP
cord-104279-choywmwd	66	7	of	of	IN
cord-104279-choywmwd	66	8	the	the	DT
cord-104279-choywmwd	66	9	DAP2	DAP2	NNP
cord-104279-choywmwd	66	10	gene	gene	NN
cord-104279-choywmwd	66	11	was	be	VBD
cord-104279-choywmwd	66	12	changed	change	VBN
cord-104279-choywmwd	66	13	to	to	IN
cord-104279-choywmwd	66	14	a	a	DT
cord-104279-choywmwd	66	15	SalI	SalI	NNP
cord-104279-choywmwd	66	16	site	site	NN
cord-104279-choywmwd	66	17	using	use	VBG
cord-104279-choywmwd	66	18	oligonucleotide	oligonucleotide	RB
cord-104279-choywmwd	66	19	-	-	HYPH
cord-104279-choywmwd	66	20	directed	direct	VBN
cord-104279-choywmwd	66	21	mutagenesis	mutagenesis	NN
cord-104279-choywmwd	66	22	(	(	-LRB-
cord-104279-choywmwd	66	23	Kunkel	Kunkel	NNP
cord-104279-choywmwd	66	24	et	et	NNP
cord-104279-choywmwd	66	25	al	al	NNP
cord-104279-choywmwd	66	26	.	.	NNP
cord-104279-choywmwd	66	27	,	,	,
cord-104279-choywmwd	66	28	1987	1987	CD
cord-104279-choywmwd	66	29	)	)	-RRB-
cord-104279-choywmwd	66	30	.	.	.
cord-104279-choywmwd	67	1	Mutagenesis	Mutagenesis	NNP
cord-104279-choywmwd	67	2	of	of	IN
cord-104279-choywmwd	67	3	DAP2	DAP2	NNP
cord-104279-choywmwd	67	4	was	be	VBD
cord-104279-choywmwd	67	5	performed	perform	VBN
cord-104279-choywmwd	67	6	using	use	VBG
cord-104279-choywmwd	67	7	the	the	DT
cord-104279-choywmwd	67	8	vector	vector	NN
cord-104279-choywmwd	68	1	pCJR27	pCJR27	NNP
cord-104279-choywmwd	68	2	,	,	,
cord-104279-choywmwd	68	3	which	which	WDT
cord-104279-choywmwd	68	4	contains	contain	VBZ
cord-104279-choywmwd	68	5	the	the	DT
cord-104279-choywmwd	68	6	3.3-kbp	3.3-kbp	CD
cord-104279-choywmwd	68	7	BamHI	BamHI	NNP
cord-104279-choywmwd	68	8	-	-	HYPH
cord-104279-choywmwd	68	9	HindIII	hindiii	NN
cord-104279-choywmwd	68	10	DA/'2	da/'2	VBP
cord-104279-choywmwd	68	11	fragment	fragment	NN
cord-104279-choywmwd	68	12	in	in	IN
cord-104279-choywmwd	68	13	the	the	DT
cord-104279-choywmwd	68	14	plasmid	plasmid	NN
cord-104279-choywmwd	68	15	KS	KS	NNP
cord-104279-choywmwd	68	16	+	+	CC
cord-104279-choywmwd	69	1	(	(	-LRB-
cord-104279-choywmwd	69	2	Stratagene	Stratagene	NNP
cord-104279-choywmwd	69	3	,	,	,
cord-104279-choywmwd	69	4	San	San	NNP
cord-104279-choywmwd	69	5	Diego	Diego	NNP
cord-104279-choywmwd	69	6	,	,	,
cord-104279-choywmwd	69	7	CA	CA	NNP
cord-104279-choywmwd	69	8	)	)	-RRB-
cord-104279-choywmwd	69	9	.	.	.
cord-104279-choywmwd	70	1	The	the	DT
cord-104279-choywmwd	70	2	2.7-kbp	2.7-kbp	CD
cord-104279-choywmwd	70	3	SalI	SalI	NNP
cord-104279-choywmwd	70	4	-	-	HYPH
cord-104279-choywmwd	70	5	HindUI	HindUI	NNP
cord-104279-choywmwd	70	6	fragment	fragment	NN
cord-104279-choywmwd	70	7	,	,	,
cord-104279-choywmwd	70	8	encoding	encode	VBG
cord-104279-choywmwd	70	9	the	the	DT
cord-104279-choywmwd	70	10	lumenal	lumenal	JJ
cord-104279-choywmwd	70	11	domain	domain	NN
cord-104279-choywmwd	70	12	of	of	IN
cord-104279-choywmwd	70	13	DPAP	DPAP	NNP
cord-104279-choywmwd	70	14	B	B	NNP
cord-104279-choywmwd	70	15	,	,	,
cord-104279-choywmwd	70	16	was	be	VBD
cord-104279-choywmwd	70	17	inserted	insert	VBN
cord-104279-choywmwd	70	18	into	into	IN
cord-104279-choywmwd	70	19	the	the	DT
cord-104279-choywmwd	70	20	SalI	SalI	NNP
cord-104279-choywmwd	70	21	-	-	HYPH
cord-104279-choywmwd	70	22	HindIII	HindIII	NNP
cord-104279-choywmwd	70	23	sites	site	NNS
cord-104279-choywmwd	70	24	of	of	IN
cord-104279-choywmwd	70	25	p771	p771	NN
cord-104279-choywmwd	70	26	/	/	SYM
cord-104279-choywmwd	70	27	SalI	SalI	NNP
cord-104279-choywmwd	70	28	,	,	,
cord-104279-choywmwd	70	29	fusing	fuse	VBG
cord-104279-choywmwd	70	30	the	the	DT
cord-104279-choywmwd	70	31	coding	code	VBG
cord-104279-choywmwd	70	32	regions	region	NNS
cord-104279-choywmwd	70	33	of	of	IN
cord-104279-choywmwd	70	34	MFal	MFal	NNP
cord-104279-choywmwd	70	35	and	and	CC
cord-104279-choywmwd	70	36	DAP2	DAP2	NNP
cord-104279-choywmwd	70	37	in	in	IN
cord-104279-choywmwd	70	38	frame	frame	NN
cord-104279-choywmwd	70	39	,	,	,
cord-104279-choywmwd	70	40	c	c	NN
cord-104279-choywmwd	70	41	~	~	NFP
cord-104279-choywmwd	70	42	Fss	Fss	NNP
cord-104279-choywmwd	70	43	-	-	HYPH
cord-104279-choywmwd	70	44	B	B	NNP
cord-104279-choywmwd	70	45	was	be	VBD
cord-104279-choywmwd	70	46	placed	place	VBN
cord-104279-choywmwd	70	47	under	under	IN
cord-104279-choywmwd	70	48	the	the	DT
cord-104279-choywmwd	70	49	control	control	NN
cord-104279-choywmwd	70	50	of	of	IN
cord-104279-choywmwd	70	51	the	the	DT
cord-104279-choywmwd	70	52	GA/_J	ga/_j	NN
cord-104279-choywmwd	70	53	promoter	promoter	NN
cord-104279-choywmwd	70	54	(	(	-LRB-
cord-104279-choywmwd	70	55	Johnston	Johnston	NNP
cord-104279-choywmwd	70	56	and	and	CC
cord-104279-choywmwd	70	57	Davis	Davis	NNP
cord-104279-choywmwd	70	58	,	,	,
cord-104279-choywmwd	70	59	1984	1984	CD
cord-104279-choywmwd	70	60	)	)	-RRB-
cord-104279-choywmwd	70	61	by	by	IN
cord-104279-choywmwd	70	62	inserting	insert	VBG
cord-104279-choywmwd	70	63	the	the	DT
cord-104279-choywmwd	70	64	2.9-kbp	2.9-kbp	CD
cord-104279-choywmwd	70	65	BamHI	BamHI	NNP
cord-104279-choywmwd	70	66	-	-	HYPH
cord-104279-choywmwd	70	67	HindIII	HindIII	NNP
cord-104279-choywmwd	70	68	fragment	fragment	NN
cord-104279-choywmwd	70	69	from	from	IN
cord-104279-choywmwd	70	70	this	this	DT
cord-104279-choywmwd	70	71	plasmid	plasmid	NN
cord-104279-choywmwd	70	72	into	into	IN
cord-104279-choywmwd	70	73	the	the	DT
cord-104279-choywmwd	70	74	BamHI	bamhi	NN
cord-104279-choywmwd	70	75	-	-	HYPH
cord-104279-choywmwd	70	76	HindIII	HindIII	NNP
cord-104279-choywmwd	70	77	sites	site	NNS
cord-104279-choywmwd	70	78	of	of	IN
cord-104279-choywmwd	70	79	pC	pC	NNP
cord-104279-choywmwd	70	80	JR52	JR52	NNP
cord-104279-choywmwd	70	81	,	,	,
cord-104279-choywmwd	70	82	which	which	WDT
cord-104279-choywmwd	70	83	contains	contain	VBZ
cord-104279-choywmwd	70	84	the	the	DT
cord-104279-choywmwd	70	85	822-bp	822-bp	CD
cord-104279-choywmwd	70	86	EcoRI	ecori	NN
cord-104279-choywmwd	70	87	-	-	HYPH
cord-104279-choywmwd	70	88	BamHI	bamhi	NN
cord-104279-choywmwd	70	89	GA/_,/	GA/_,/	NNP
cord-104279-choywmwd	70	90	promoter	promoter	NN
cord-104279-choywmwd	70	91	fragment	fragment	NN
cord-104279-choywmwd	70	92	inserted	insert	VBN
cord-104279-choywmwd	70	93	into	into	IN
cord-104279-choywmwd	70	94	EcoRI	ecori	NN
cord-104279-choywmwd	70	95	-	-	HYPH
cord-104279-choywmwd	70	96	BamHI	bamhi	NN
cord-104279-choywmwd	70	97	sites	site	NNS
cord-104279-choywmwd	70	98	of	of	IN
cord-104279-choywmwd	70	99	the	the	DT
cord-104279-choywmwd	70	100	CEN	CEN	NNP
cord-104279-choywmwd	70	101	plasmid	plasmid	NN
cord-104279-choywmwd	70	102	pSEYC68	pseyc68	NN
cord-104279-choywmwd	71	1	Ca	Ca	NNP
cord-104279-choywmwd	71	2	modified	modify	VBN
cord-104279-choywmwd	71	3	version	version	NN
cord-104279-choywmwd	71	4	of	of	IN
cord-104279-choywmwd	71	5	pSEYC58	pseyc58	NN
cord-104279-choywmwd	71	6	;	;	:
cord-104279-choywmwd	71	7	Emr	Emr	NNP
cord-104279-choywmwd	71	8	et	et	NNP
cord-104279-choywmwd	71	9	al	al	NNP
cord-104279-choywmwd	71	10	.	.	NNP
cord-104279-choywmwd	71	11	,	,	,
cord-104279-choywmwd	71	12	1983	1983	CD
cord-104279-choywmwd	71	13	)	)	-RRB-
cord-104279-choywmwd	71	14	.	.	.
cord-104279-choywmwd	72	1	The	the	DT
cord-104279-choywmwd	72	2	resulting	result	VBG
cord-104279-choywmwd	72	3	fusion	fusion	NN
cord-104279-choywmwd	72	4	protein	protein	NN
cord-104279-choywmwd	72	5	consists	consist	VBZ
cord-104279-choywmwd	72	6	of	of	IN
cord-104279-choywmwd	72	7	the	the	DT
cord-104279-choywmwd	72	8	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	72	9	22	22	CD
cord-104279-choywmwd	72	10	residues	residue	NNS
cord-104279-choywmwd	72	11	of	of	IN
cord-104279-choywmwd	72	12	prepro	prepro	NN
cord-104279-choywmwd	72	13	-	-	HYPH
cord-104279-choywmwd	72	14	afactor	afactor	NN
cord-104279-choywmwd	72	15	and	and	CC
cord-104279-choywmwd	72	16	two	two	CD
cord-104279-choywmwd	72	17	residues	residue	NNS
cord-104279-choywmwd	72	18	arising	arise	VBG
cord-104279-choywmwd	72	19	from	from	IN
cord-104279-choywmwd	72	20	linker	linker	NN
cord-104279-choywmwd	72	21	sequences	sequence	NNS
cord-104279-choywmwd	72	22	fused	fuse	VBN
cord-104279-choywmwd	72	23	to	to	IN
cord-104279-choywmwd	72	24	residue	residue	NN
cord-104279-choywmwd	72	25	49	49	CD
cord-104279-choywmwd	72	26	of	of	IN
cord-104279-choywmwd	72	27	the	the	DT
cord-104279-choywmwd	72	28	lumenal	lumenal	JJ
cord-104279-choywmwd	72	29	domain	domain	NN
cord-104279-choywmwd	72	30	of	of	IN
cord-104279-choywmwd	72	31	DPAP	DPAP	NNP
cord-104279-choywmwd	72	32	B	B	NNP
cord-104279-choywmwd	72	33	(	(	-LRB-
cord-104279-choywmwd	72	34	Fig	Fig	NNP
cord-104279-choywmwd	72	35	.	.	.
cord-104279-choywmwd	73	1	l	l	NNP
cord-104279-choywmwd	73	2	B	B	NNP
cord-104279-choywmwd	73	3	;	;	:
cord-104279-choywmwd	73	4	amino	amino	NN
cord-104279-choywmwd	73	5	acid	acid	NN
cord-104279-choywmwd	73	6	sequence	sequence	NN
cord-104279-choywmwd	73	7	NH2-MRFPSIFTAVLFAASSALA	NH2-MRFPSIFTAVLFAASSALA	NNP
cord-104279-choywmwd	73	8	-	-	HYPH
cord-104279-choywmwd	73	9	APVGRPHH	APVGRPHH	NNP
cord-104279-choywmwd	73	10	.	.	.
cord-104279-choywmwd	73	11	.	.	.
cord-104279-choywmwd	73	12	.	.	.
cord-104279-choywmwd	73	13	)	)	-RRB-
cord-104279-choywmwd	73	14	.	.	.
cord-104279-choywmwd	74	1	The	the	DT
cord-104279-choywmwd	74	2	BB	BB	NNP
cord-104279-choywmwd	74	3	-	-	HYPH
cord-104279-choywmwd	74	4	Inv	inv	NN
cord-104279-choywmwd	74	5	fusion	fusion	NN
cord-104279-choywmwd	74	6	protein	protein	NN
cord-104279-choywmwd	74	7	expression	expression	NN
cord-104279-choywmwd	74	8	vectors	vector	NNS
cord-104279-choywmwd	74	9	,	,	,
cord-104279-choywmwd	74	10	pCJR13	pcjr13	NN
cord-104279-choywmwd	74	11	and	and	CC
cord-104279-choywmwd	74	12	pC	pc	VB
cord-104279-choywmwd	74	13	JR15	JR15	NNP
cord-104279-choywmwd	75	1	(	(	-LRB-
cord-104279-choywmwd	75	2	2p	2p	CD
cord-104279-choywmwd	75	3	and	and	CC
cord-104279-choywmwd	75	4	CEN	CEN	NNP
cord-104279-choywmwd	75	5	plasmids	plasmid	NNS
cord-104279-choywmwd	75	6	,	,	,
cord-104279-choywmwd	75	7	respectively	respectively	RB
cord-104279-choywmwd	75	8	)	)	-RRB-
cord-104279-choywmwd	75	9	,	,	,
cord-104279-choywmwd	75	10	were	be	VBD
cord-104279-choywmwd	75	11	constructed	construct	VBN
cord-104279-choywmwd	75	12	by	by	IN
cord-104279-choywmwd	75	13	inserting	insert	VBG
cord-104279-choywmwd	75	14	the	the	DT
cord-104279-choywmwd	75	15	0.6-kbp	0.6-kbp	CD
cord-104279-choywmwd	75	16	BamHI	BamHI	NNP
cord-104279-choywmwd	75	17	-	-	HYPH
cord-104279-choywmwd	75	18	AccI	AccI	NNP
cord-104279-choywmwd	75	19	fragment	fragment	NN
cord-104279-choywmwd	75	20	(	(	-LRB-
cord-104279-choywmwd	75	21	AccI	AccI	NNP
cord-104279-choywmwd	75	22	blunt	blunt	NNP
cord-104279-choywmwd	75	23	ended	end	VBD
cord-104279-choywmwd	75	24	)	)	-RRB-
cord-104279-choywmwd	75	25	into	into	IN
cord-104279-choywmwd	75	26	the	the	DT
cord-104279-choywmwd	75	27	invertase	invertase	NNP
cord-104279-choywmwd	75	28	fusion	fusion	NN
cord-104279-choywmwd	75	29	vectors	vector	NNS
cord-104279-choywmwd	75	30	pSEY304	pSEY304	NNP
cord-104279-choywmwd	75	31	(	(	-LRB-
cord-104279-choywmwd	75	32	a	a	DT
cord-104279-choywmwd	75	33	derivative	derivative	NN
cord-104279-choywmwd	75	34	of	of	IN
cord-104279-choywmwd	75	35	the	the	DT
cord-104279-choywmwd	75	36	2	2	CD
cord-104279-choywmwd	75	37	/	/	SYM
cord-104279-choywmwd	75	38	z	z	NN
cord-104279-choywmwd	75	39	plasmid	plasmid	NN
cord-104279-choywmwd	75	40	pSEY303	psey303	RB
cord-104279-choywmwd	75	41	;	;	.
cord-104279-choywmwd	76	1	Emr	Emr	NNP
cord-104279-choywmwd	76	2	et	et	NNP
cord-104279-choywmwd	76	3	al	al	NNP
cord-104279-choywmwd	76	4	.	.	NNP
cord-104279-choywmwd	76	5	,	,	,
cord-104279-choywmwd	76	6	1986	1986	CD
cord-104279-choywmwd	76	7	)	)	-RRB-
cord-104279-choywmwd	76	8	and	and	CC
cord-104279-choywmwd	76	9	the	the	DT
cord-104279-choywmwd	76	10	CEN	CEN	NNP
cord-104279-choywmwd	76	11	plasmid	plasmid	NN
cord-104279-choywmwd	76	12	pSEYC306	pseyc306	NN
cord-104279-choywmwd	77	1	(	(	-LRB-
cord-104279-choywmwd	77	2	Johnson	Johnson	NNP
cord-104279-choywmwd	77	3	et	et	NNP
cord-104279-choywmwd	77	4	al	al	NNP
cord-104279-choywmwd	77	5	.	.	NNP
cord-104279-choywmwd	77	6	,	,	,
cord-104279-choywmwd	77	7	1987	1987	CD
cord-104279-choywmwd	77	8	)	)	-RRB-
cord-104279-choywmwd	77	9	at	at	IN
cord-104279-choywmwd	77	10	the	the	DT
cord-104279-choywmwd	77	11	BamHI	BamHI	NNP
cord-104279-choywmwd	77	12	and	and	CC
cord-104279-choywmwd	77	13	Hin	Hin	NNP
cord-104279-choywmwd	77	14	-	-	HYPH
cord-104279-choywmwd	77	15	dIII	diii	NN
cord-104279-choywmwd	77	16	sites	site	NNS
cord-104279-choywmwd	77	17	(	(	-LRB-
cord-104279-choywmwd	77	18	HindlIJ	hindlij	JJ
cord-104279-choywmwd	77	19	blunt	blunt	NN
cord-104279-choywmwd	77	20	ended	end	VBD
cord-104279-choywmwd	77	21	)	)	-RRB-
cord-104279-choywmwd	77	22	.	.	.
cord-104279-choywmwd	78	1	The	the	DT
cord-104279-choywmwd	78	2	resulting	result	VBG
cord-104279-choywmwd	78	3	fusion	fusion	NN
cord-104279-choywmwd	78	4	protein	protein	NN
cord-104279-choywmwd	78	5	contains	contain	VBZ
cord-104279-choywmwd	78	6	the	the	DT
cord-104279-choywmwd	78	7	48	48	CD
cord-104279-choywmwd	78	8	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	78	9	residues	residue	NNS
cord-104279-choywmwd	78	10	of	of	IN
cord-104279-choywmwd	78	11	DPAP	DPAP	NNP
cord-104279-choywmwd	78	12	B	B	NNP
cord-104279-choywmwd	78	13	fused	fuse	VBD
cord-104279-choywmwd	78	14	to	to	IN
cord-104279-choywmwd	78	15	residue	residue	NN
cord-104279-choywmwd	78	16	three	three	CD
cord-104279-choywmwd	78	17	of	of	IN
cord-104279-choywmwd	78	18	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	78	19	invertase	invertase	NN
cord-104279-choywmwd	78	20	(	(	-LRB-
cord-104279-choywmwd	78	21	Fig	Fig	NNP
cord-104279-choywmwd	78	22	.	.	.
cord-104279-choywmwd	78	23	1	1	CD
cord-104279-choywmwd	78	24	B	b	NN
cord-104279-choywmwd	78	25	)	)	-RRB-
cord-104279-choywmwd	78	26	.	.	.
cord-104279-choywmwd	79	1	BB	BB	NNP
cord-104279-choywmwd	79	2	-	-	HYPH
cord-104279-choywmwd	79	3	Inv	Inv	NNP
cord-104279-choywmwd	79	4	was	be	VBD
cord-104279-choywmwd	79	5	placed	place	VBN
cord-104279-choywmwd	79	6	under	under	IN
cord-104279-choywmwd	79	7	the	the	DT
cord-104279-choywmwd	79	8	control	control	NN
cord-104279-choywmwd	79	9	of	of	IN
cord-104279-choywmwd	79	10	the	the	DT
cord-104279-choywmwd	79	11	GAL	GAL	NNP
cord-104279-choywmwd	79	12	/	/	SYM
cord-104279-choywmwd	79	13	promoter	promoter	NN
cord-104279-choywmwd	79	14	by	by	IN
cord-104279-choywmwd	79	15	cutting	cut	VBG
cord-104279-choywmwd	79	16	pCJR15	pCJR15	NNP
cord-104279-choywmwd	79	17	with	with	IN
cord-104279-choywmwd	79	18	EcoRI	ecori	NN
cord-104279-choywmwd	79	19	and	and	CC
cord-104279-choywmwd	79	20	HiedIII	HiedIII	NNP
cord-104279-choywmwd	79	21	and	and	CC
cord-104279-choywmwd	79	22	ligating	ligate	VBG
cord-104279-choywmwd	79	23	in	in	IN
cord-104279-choywmwd	79	24	the	the	DT
cord-104279-choywmwd	79	25	822-bp	822-bp	CD
cord-104279-choywmwd	79	26	GAL	gal	NN
cord-104279-choywmwd	79	27	/	/	SYM
cord-104279-choywmwd	79	28	fragment	fragment	NN
cord-104279-choywmwd	79	29	(	(	-LRB-
cord-104279-choywmwd	79	30	Johnston	Johnston	NNP
cord-104279-choywmwd	79	31	and	and	CC
cord-104279-choywmwd	79	32	Davis	Davis	NNP
cord-104279-choywmwd	79	33	,	,	,
cord-104279-choywmwd	79	34	1984	1984	CD
cord-104279-choywmwd	79	35	)	)	-RRB-
cord-104279-choywmwd	79	36	.	.	.
cord-104279-choywmwd	80	1	This	this	DT
cord-104279-choywmwd	80	2	fused	fuse	VBD
cord-104279-choywmwd	80	3	the	the	DT
cord-104279-choywmwd	80	4	3	3	CD
cord-104279-choywmwd	80	5	'	'	NN
cord-104279-choywmwd	80	6	end	end	NN
cord-104279-choywmwd	80	7	of	of	IN
cord-104279-choywmwd	80	8	the	the	DT
cord-104279-choywmwd	80	9	GAL	gal	NN
cord-104279-choywmwd	80	10	/	/	SYM
cord-104279-choywmwd	80	11	fragment	fragment	NN
cord-104279-choywmwd	80	12	at	at	IN
cord-104279-choywmwd	80	13	nucleotide	nucleotide	NN
cord-104279-choywmwd	80	14	-92	-92	NNP
cord-104279-choywmwd	80	15	of	of	IN
cord-104279-choywmwd	80	16	the	the	DT
cord-104279-choywmwd	80	17	DAP2	DAP2	NNP
cord-104279-choywmwd	80	18	sequence	sequence	NN
cord-104279-choywmwd	80	19	.	.	.
cord-104279-choywmwd	81	1	Vectors	vector	NNS
cord-104279-choywmwd	81	2	encoding	encode	VBG
cord-104279-choywmwd	81	3	A20-BB	a20-bb	NN
cord-104279-choywmwd	81	4	and	and	CC
cord-104279-choywmwd	81	5	A27-BB	A27-BB	NNP
cord-104279-choywmwd	81	6	were	be	VBD
cord-104279-choywmwd	81	7	constructed	construct	VBN
cord-104279-choywmwd	81	8	as	as	IN
cord-104279-choywmwd	81	9	follows	follow	NNS
cord-104279-choywmwd	81	10	.	.	.
cord-104279-choywmwd	82	1	Oligonucleotide	Oligonucleotide	NNP
cord-104279-choywmwd	82	2	-	-	HYPH
cord-104279-choywmwd	82	3	directed	direct	VBN
cord-104279-choywmwd	82	4	mutagenesis	mutagenesis	NN
cord-104279-choywmwd	82	5	(	(	-LRB-
cord-104279-choywmwd	82	6	Kunkel	Kunkel	NNP
cord-104279-choywmwd	82	7	et	et	NNP
cord-104279-choywmwd	82	8	al	al	NNP
cord-104279-choywmwd	82	9	.	.	NNP
cord-104279-choywmwd	82	10	,	,	,
cord-104279-choywmwd	82	11	1987	1987	CD
cord-104279-choywmwd	82	12	)	)	-RRB-
cord-104279-choywmwd	82	13	was	be	VBD
cord-104279-choywmwd	82	14	used	use	VBN
cord-104279-choywmwd	82	15	to	to	TO
cord-104279-choywmwd	82	16	create	create	VB
cord-104279-choywmwd	82	17	60-and	60-and	CD
cord-104279-choywmwd	82	18	81-bp	81-bp	CD
cord-104279-choywmwd	82	19	deletions	deletion	NNS
cord-104279-choywmwd	82	20	in	in	IN
cord-104279-choywmwd	82	21	DAP2	DAP2	NNP
cord-104279-choywmwd	82	22	(	(	-LRB-
cord-104279-choywmwd	82	23	A20-BB	a20-bb	NN
cord-104279-choywmwd	82	24	and	and	CC
cord-104279-choywmwd	82	25	A27-BB	A27-BB	NNP
cord-104279-choywmwd	82	26	,	,	,
cord-104279-choywmwd	82	27	respectively	respectively	RB
cord-104279-choywmwd	82	28	)	)	-RRB-
cord-104279-choywmwd	82	29	in	in	IN
cord-104279-choywmwd	82	30	pCJR27	pCJR27	NNP
cord-104279-choywmwd	82	31	.	.	.
cord-104279-choywmwd	83	1	The	the	DT
cord-104279-choywmwd	83	2	~3.2kbp	~3.2kbp	NFP
cord-104279-choywmwd	83	3	BamHI	BamHI	NNP
cord-104279-choywmwd	83	4	-	-	HYPH
cord-104279-choywmwd	83	5	HindIII	HindIII	NNP
cord-104279-choywmwd	83	6	mutant	mutant	JJ
cord-104279-choywmwd	83	7	DAP2	DAP2	NNP
cord-104279-choywmwd	83	8	fragments	fragment	NNS
cord-104279-choywmwd	83	9	were	be	VBD
cord-104279-choywmwd	83	10	inserted	insert	VBN
cord-104279-choywmwd	83	11	into	into	IN
cord-104279-choywmwd	83	12	the	the	DT
cord-104279-choywmwd	83	13	BamHI	bamhi	NN
cord-104279-choywmwd	83	14	-	-	HYPH
cord-104279-choywmwd	83	15	HindIII	HindIII	NNP
cord-104279-choywmwd	83	16	sites	site	NNS
cord-104279-choywmwd	83	17	of	of	IN
cord-104279-choywmwd	83	18	the	the	DT
cord-104279-choywmwd	83	19	CEN	CEN	NNP
cord-104279-choywmwd	83	20	plasmid	plasmid	NN
cord-104279-choywmwd	83	21	pSEYC58	pseyc58	NN
cord-104279-choywmwd	83	22	,	,	,
cord-104279-choywmwd	83	23	creating	create	VBG
cord-104279-choywmwd	83	24	pMNH1	pmnh1	IN
cord-104279-choywmwd	83	25	(	(	-LRB-
cord-104279-choywmwd	83	26	620-BB	620-bb	CD
cord-104279-choywmwd	83	27	)	)	-RRB-
cord-104279-choywmwd	83	28	and	and	CC
cord-104279-choywmwd	83	29	pC	pc	JJ
cord-104279-choywmwd	83	30	JR45	JR45	NNP
cord-104279-choywmwd	83	31	(	(	-LRB-
cord-104279-choywmwd	83	32	A27-BB	A27-BB	NNP
cord-104279-choywmwd	83	33	)	)	-RRB-
cord-104279-choywmwd	83	34	.	.	.
cord-104279-choywmwd	84	1	&	&	CC
cord-104279-choywmwd	84	2	20-BB	20-bb	CD
cord-104279-choywmwd	84	3	and	and	CC
cord-104279-choywmwd	84	4	A27-BB	A27-BB	NNP
cord-104279-choywmwd	84	5	were	be	VBD
cord-104279-choywmwd	84	6	over	over	RB
cord-104279-choywmwd	84	7	-	-	HYPH
cord-104279-choywmwd	84	8	produced	produce	VBN
cord-104279-choywmwd	84	9	by	by	IN
cord-104279-choywmwd	84	10	replacing	replace	VBG
cord-104279-choywmwd	84	11	the	the	DT
cord-104279-choywmwd	84	12	DAP2	DAP2	NNP
cord-104279-choywmwd	84	13	promoter	promoter	NN
cord-104279-choywmwd	84	14	with	with	IN
cord-104279-choywmwd	84	15	the	the	DT
cord-104279-choywmwd	84	16	GAL	GAL	NNP
cord-104279-choywmwd	84	17	/	/	SYM
cord-104279-choywmwd	84	18	promoter	promoter	NN
cord-104279-choywmwd	84	19	as	as	IN
cord-104279-choywmwd	84	20	follows	follow	VBZ
cord-104279-choywmwd	84	21	.	.	.
cord-104279-choywmwd	85	1	The	the	DT
cord-104279-choywmwd	85	2	HindIII	HindIII	NNPS
cord-104279-choywmwd	85	3	site	site	NN
cord-104279-choywmwd	85	4	at	at	IN
cord-104279-choywmwd	85	5	-92	-92	CD
cord-104279-choywmwd	85	6	of	of	IN
cord-104279-choywmwd	85	7	DAP2	DAP2	NNP
cord-104279-choywmwd	85	8	was	be	VBD
cord-104279-choywmwd	85	9	blunt	blunt	JJ
cord-104279-choywmwd	85	10	ended	end	VBN
cord-104279-choywmwd	85	11	and	and	CC
cord-104279-choywmwd	85	12	religated	religated	JJ
cord-104279-choywmwd	85	13	,	,	,
cord-104279-choywmwd	85	14	creating	create	VBG
cord-104279-choywmwd	85	15	an	an	DT
cord-104279-choywmwd	85	16	NheI	NheI	NNP
cord-104279-choywmwd	85	17	site	site	NN
cord-104279-choywmwd	85	18	.	.	.
cord-104279-choywmwd	86	1	The	the	DT
cord-104279-choywmwd	86	2	~3-kbp	~3-kbp	NNP
cord-104279-choywmwd	86	3	NheI	NheI	NNP
cord-104279-choywmwd	86	4	-	-	HYPH
cord-104279-choywmwd	86	5	HindIII	hindiii	NN
cord-104279-choywmwd	86	6	fragments	fragment	NNS
cord-104279-choywmwd	86	7	of	of	IN
cord-104279-choywmwd	86	8	pC	pc	NN
cord-104279-choywmwd	86	9	JR43	JR43	NNP
cord-104279-choywmwd	87	1	and	and	CC
cord-104279-choywmwd	87	2	pC	pC	NNP
cord-104279-choywmwd	87	3	JR44	JR44	NNP
cord-104279-choywmwd	87	4	were	be	VBD
cord-104279-choywmwd	87	5	inserted	insert	VBN
cord-104279-choywmwd	87	6	at	at	IN
cord-104279-choywmwd	87	7	the	the	DT
cord-104279-choywmwd	87	8	XbaI	XbaI	NNP
cord-104279-choywmwd	87	9	-	-	HYPH
cord-104279-choywmwd	87	10	HindIII	HindIII	NNP
cord-104279-choywmwd	87	11	sites	site	NNS
cord-104279-choywmwd	87	12	of	of	IN
cord-104279-choywmwd	87	13	pC	pC	NNP
cord-104279-choywmwd	87	14	JR52	JR52	NNP
cord-104279-choywmwd	87	15	,	,	,
cord-104279-choywmwd	87	16	creating	create	VBG
cord-104279-choywmwd	87	17	pC	pc	NN
cord-104279-choywmwd	88	1	JR56	JR56	NNP
cord-104279-choywmwd	89	1	and	and	CC
cord-104279-choywmwd	89	2	pC	pC	NNP
cord-104279-choywmwd	89	3	JR54	JR54	NNP
cord-104279-choywmwd	89	4	,	,	,
cord-104279-choywmwd	89	5	respectively	respectively	RB
cord-104279-choywmwd	89	6	.	.	.
cord-104279-choywmwd	90	1	The	the	DT
cord-104279-choywmwd	90	2	A20	A20	NNP
cord-104279-choywmwd	90	3	and	and	CC
cord-104279-choywmwd	90	4	A27	A27	NNP
cord-104279-choywmwd	90	5	deletions	deletion	NNS
cord-104279-choywmwd	90	6	removed	remove	VBD
cord-104279-choywmwd	90	7	residues	residue	NNS
cord-104279-choywmwd	90	8	2	2	CD
cord-104279-choywmwd	90	9	-	-	SYM
cord-104279-choywmwd	90	10	21	21	CD
cord-104279-choywmwd	90	11	and	and	CC
cord-104279-choywmwd	90	12	2	2	CD
cord-104279-choywmwd	90	13	-	-	SYM
cord-104279-choywmwd	90	14	27	27	CD
cord-104279-choywmwd	90	15	of	of	IN
cord-104279-choywmwd	90	16	DPAP	DPAP	NNP
cord-104279-choywmwd	90	17	B	B	NNP
cord-104279-choywmwd	90	18	,	,	,
cord-104279-choywmwd	90	19	changing	change	VBG
cord-104279-choywmwd	90	20	the	the	DT
cord-104279-choywmwd	90	21	NH2-terr	NH2-terr	NNP
cord-104279-choywmwd	90	22	~	~	.
cord-104279-choywmwd	90	23	nal	nal	JJ
cord-104279-choywmwd	90	24	acid	acid	JJ
cord-104279-choywmwd	90	25	sequence	sequence	NN
cord-104279-choywmwd	90	26	of	of	IN
cord-104279-choywmwd	90	27	DPAP	DPAP	NNP
cord-104279-choywmwd	90	28	B	B	NNP
cord-104279-choywmwd	90	29	from	from	IN
cord-104279-choywmwd	90	30	NH2-MEG	NH2-MEG	NNP
cord-104279-choywmwd	90	31	-	-	HYPH
cord-104279-choywmwd	90	32	GEEEVERIPDELFDTKKKHLLDKLIRV30	GEEEVERIPDELFDTKKKHLLDKLIRV30	NNP
cord-104279-choywmwd	90	33	to	to	IN
cord-104279-choywmwd	90	34	NH2-MHLLDKLIRV	NH2-MHLLDKLIRV	NNP
cord-104279-choywmwd	90	35	and	and	CC
cord-104279-choywmwd	90	36	NH2-MIRV	NH2-MIRV	NNP
cord-104279-choywmwd	90	37	,	,	,
cord-104279-choywmwd	90	38	respectively	respectively	RB
cord-104279-choywmwd	90	39	(	(	-LRB-
cord-104279-choywmwd	90	40	Fig	fig	NN
cord-104279-choywmwd	90	41	.	.	.
cord-104279-choywmwd	90	42	1	1	CD
cord-104279-choywmwd	90	43	B	b	NN
cord-104279-choywmwd	90	44	)	)	-RRB-
cord-104279-choywmwd	90	45	.	.	.
cord-104279-choywmwd	91	1	The	the	DT
cord-104279-choywmwd	91	2	2pt	2pt	JJ
cord-104279-choywmwd	91	3	plasmid	plasmid	NN
cord-104279-choywmwd	91	4	encoding	encoding	NN
cord-104279-choywmwd	91	5	DPAP	DPAP	NNP
cord-104279-choywmwd	91	6	A	A	NNP
cord-104279-choywmwd	91	7	,	,	,
cord-104279-choywmwd	91	8	pCJR46	pCJR46	NNP
cord-104279-choywmwd	91	9	,	,	,
cord-104279-choywmwd	91	10	was	be	VBD
cord-104279-choywmwd	91	11	constructed	construct	VBN
cord-104279-choywmwd	91	12	as	as	IN
cord-104279-choywmwd	91	13	follows	follow	VBZ
cord-104279-choywmwd	91	14	:	:	:
cord-104279-choywmwd	91	15	the	the	DT
cord-104279-choywmwd	91	16	5.9-kbp	5.9-kbp	CD
cord-104279-choywmwd	91	17	XbaI	XbaI	NNP
cord-104279-choywmwd	91	18	-	-	HYPH
cord-104279-choywmwd	91	19	BamHI	bamhi	NN
cord-104279-choywmwd	91	20	fragment	fragment	NN
cord-104279-choywmwd	91	21	,	,	,
cord-104279-choywmwd	91	22	containing	contain	VBG
cord-104279-choywmwd	91	23	the	the	DT
cord-104279-choywmwd	91	24	STF_J3	stf_j3	JJ
cord-104279-choywmwd	91	25	gene	gene	NN
cord-104279-choywmwd	91	26	(	(	-LRB-
cord-104279-choywmwd	91	27	C.	C.	NNP
cord-104279-choywmwd	91	28	A.	A.	NNP
cord-104279-choywmwd	91	29	Flanagan	Flanagan	NNP
cord-104279-choywmwd	91	30	,	,	,
cord-104279-choywmwd	91	31	D.	D.	NNP
cord-104279-choywmwd	91	32	A.	A.	NNP
cord-104279-choywmwd	91	33	Barnes	Barnes	NNP
cord-104279-choywmwd	91	34	,	,	,
cord-104279-choywmwd	91	35	M.	M.	NNP
cord-104279-choywmwd	91	36	C.	C.	NNP
cord-104279-choywmwd	91	37	Flessel	Flessel	NNP
cord-104279-choywmwd	91	38	,	,	,
cord-104279-choywmwd	92	1	andJ.	andJ.	NNP
cord-104279-choywmwd	92	2	Thorner	Thorner	NNP
cord-104279-choywmwd	92	3	,	,	,
cord-104279-choywmwd	92	4	manuscript	manuscript	NN
cord-104279-choywmwd	92	5	submitted	submit	VBN
cord-104279-choywmwd	92	6	for	for	IN
cord-104279-choywmwd	92	7	publication	publication	NN
cord-104279-choywmwd	92	8	)	)	-RRB-
cord-104279-choywmwd	92	9	was	be	VBD
cord-104279-choywmwd	92	10	isolated	isolate	VBN
cord-104279-choywmwd	92	11	from	from	IN
cord-104279-choywmwd	92	12	the	the	DT
cord-104279-choywmwd	92	13	plasmid	plasmid	NN
cord-104279-choywmwd	92	14	p13	p13	NN
cord-104279-choywmwd	92	15	-	-	HYPH
cord-104279-choywmwd	92	16	3	3	CD
cord-104279-choywmwd	92	17	(	(	-LRB-
cord-104279-choywmwd	92	18	Julius	Julius	NNP
cord-104279-choywmwd	92	19	et	et	NNP
cord-104279-choywmwd	92	20	al	al	NNP
cord-104279-choywmwd	92	21	.	.	NNP
cord-104279-choywmwd	92	22	,	,	,
cord-104279-choywmwd	92	23	1983	1983	CD
cord-104279-choywmwd	92	24	)	)	-RRB-
cord-104279-choywmwd	92	25	and	and	CC
cord-104279-choywmwd	92	26	inserted	insert	VBD
cord-104279-choywmwd	92	27	into	into	IN
cord-104279-choywmwd	92	28	pUC13	pUC13	NNS
cord-104279-choywmwd	92	29	.	.	.
cord-104279-choywmwd	93	1	The	the	DT
cord-104279-choywmwd	93	2	5.9-kbp	5.9-kbp	CD
cord-104279-choywmwd	93	3	SalI	SalI	NNP
cord-104279-choywmwd	93	4	-	-	HYPH
cord-104279-choywmwd	93	5	BamI	BamI	NNP
cord-104279-choywmwd	93	6	-	-	HYPH
cord-104279-choywmwd	93	7	II	II	NNP
cord-104279-choywmwd	93	8	fragment	fragment	NN
cord-104279-choywmwd	93	9	from	from	IN
cord-104279-choywmwd	93	10	this	this	DT
cord-104279-choywmwd	93	11	vector	vector	NN
cord-104279-choywmwd	93	12	was	be	VBD
cord-104279-choywmwd	93	13	inserted	insert	VBN
cord-104279-choywmwd	93	14	into	into	IN
cord-104279-choywmwd	93	15	the	the	DT
cord-104279-choywmwd	93	16	XhoI	XhoI	NNP
cord-104279-choywmwd	93	17	-	-	HYPH
cord-104279-choywmwd	93	18	BglII	bglii	NN
cord-104279-choywmwd	93	19	sites	site	NNS
cord-104279-choywmwd	93	20	of	of	IN
cord-104279-choywmwd	93	21	the	the	DT
cord-104279-choywmwd	93	22	2	2	CD
cord-104279-choywmwd	93	23	#	#	NN
cord-104279-choywmwd	93	24	plasmid	plasmid	NN
cord-104279-choywmwd	93	25	pCKR201	pckr201	CD
cord-104279-choywmwd	94	1	(	(	-LRB-
cord-104279-choywmwd	94	2	C.	C.	NNP
cord-104279-choywmwd	94	3	Raymond	Raymond	NNP
cord-104279-choywmwd	94	4	and	and	CC
cord-104279-choywmwd	94	5	T.	T.	NNP
cord-104279-choywmwd	94	6	Stevens	Stevens	NNP
cord-104279-choywmwd	94	7	,	,	,
cord-104279-choywmwd	94	8	unpublished	unpublished	JJ
cord-104279-choywmwd	94	9	results	result	NNS
cord-104279-choywmwd	94	10	)	)	-RRB-
cord-104279-choywmwd	94	11	.	.	.
cord-104279-choywmwd	95	1	The	the	DT
cord-104279-choywmwd	95	2	CEN	CEN	NNP
cord-104279-choywmwd	95	3	plasmid	plasmid	NN
cord-104279-choywmwd	95	4	pCJR78	pCJR78	NNP
cord-104279-choywmwd	95	5	was	be	VBD
cord-104279-choywmwd	95	6	made	make	VBN
cord-104279-choywmwd	95	7	by	by	IN
cord-104279-choywmwd	95	8	inserting	insert	VBG
cord-104279-choywmwd	95	9	the	the	DT
cord-104279-choywmwd	95	10	4.5-kbp	4.5-kbp	CD
cord-104279-choywmwd	95	11	EagI	EagI	NNP
cord-104279-choywmwd	95	12	-	-	HYPH
cord-104279-choywmwd	95	13	PvuII	PvuII	NNP
cord-104279-choywmwd	95	14	STE/3	STE/3	NNP
cord-104279-choywmwd	95	15	fragment	fragment	NN
cord-104279-choywmwd	95	16	into	into	IN
cord-104279-choywmwd	95	17	the	the	DT
cord-104279-choywmwd	95	18	EagLEcoRV	EagLEcoRV	NNP
cord-104279-choywmwd	95	19	sites	site	NNS
cord-104279-choywmwd	95	20	of	of	IN
cord-104279-choywmwd	95	21	pRS316	prs316	CD
cord-104279-choywmwd	96	1	(	(	-LRB-
cord-104279-choywmwd	96	2	Sikorski	Sikorski	NNP
cord-104279-choywmwd	96	3	and	and	CC
cord-104279-choywmwd	96	4	Hieter	Hieter	NNP
cord-104279-choywmwd	96	5	,	,	,
cord-104279-choywmwd	96	6	1989	1989	CD
cord-104279-choywmwd	96	7	)	)	-RRB-
cord-104279-choywmwd	96	8	.	.	.
cord-104279-choywmwd	97	1	The	the	DT
cord-104279-choywmwd	97	2	plasmid	plasmid	NN
cord-104279-choywmwd	97	3	pC	pc	NN
cord-104279-choywmwd	97	4	JR64	JR64	NNP
cord-104279-choywmwd	97	5	,	,	,
cord-104279-choywmwd	97	6	encoding	encode	VBG
cord-104279-choywmwd	97	7	the	the	DT
cord-104279-choywmwd	97	8	fusion	fusion	NN
cord-104279-choywmwd	97	9	protein	protein	NN
cord-104279-choywmwd	97	10	AA	AA	NNP
cord-104279-choywmwd	97	11	-	-	HYPH
cord-104279-choywmwd	97	12	B	B	NNP
cord-104279-choywmwd	97	13	,	,	,
cord-104279-choywmwd	97	14	was	be	VBD
cord-104279-choywmwd	97	15	constructed	construct	VBN
cord-104279-choywmwd	97	16	by	by	IN
cord-104279-choywmwd	97	17	inserting	insert	VBG
cord-104279-choywmwd	97	18	the	the	DT
cord-104279-choywmwd	97	19	2.5-kbp	2.5-kbp	CD
cord-104279-choywmwd	97	20	BamHI	BamHI	NNP
cord-104279-choywmwd	97	21	-	-	HYPH
cord-104279-choywmwd	97	22	MluI	MluI	NNP
cord-104279-choywmwd	97	23	(	(	-LRB-
cord-104279-choywmwd	97	24	MluI	MluI	NNP
cord-104279-choywmwd	97	25	blunt	blunt	NNP
cord-104279-choywmwd	97	26	ended	end	VBD
cord-104279-choywmwd	97	27	)	)	-RRB-
cord-104279-choywmwd	97	28	fragment	fragment	NN
cord-104279-choywmwd	97	29	from	from	IN
cord-104279-choywmwd	97	30	p13	p13	CD
cord-104279-choywmwd	97	31	-	-	SYM
cord-104279-choywmwd	97	32	3	3	CD
cord-104279-choywmwd	97	33	,	,	,
cord-104279-choywmwd	97	34	encoding	encode	VBG
cord-104279-choywmwd	97	35	the	the	DT
cord-104279-choywmwd	97	36	STF.J3	STF.J3	NNP
cord-104279-choywmwd	97	37	promoter	promoter	NN
cord-104279-choywmwd	97	38	and	and	CC
cord-104279-choywmwd	97	39	the	the	DT
cord-104279-choywmwd	97	40	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	97	41	and	and	CC
cord-104279-choywmwd	97	42	trans-	trans-	JJ
cord-104279-choywmwd	97	43	membrane	membrane	NN
cord-104279-choywmwd	97	44	domains	domain	NNS
cord-104279-choywmwd	97	45	of	of	IN
cord-104279-choywmwd	97	46	DPAP	DPAP	NNP
cord-104279-choywmwd	97	47	A	A	NNP
cord-104279-choywmwd	97	48	,	,	,
cord-104279-choywmwd	97	49	and	and	CC
cord-104279-choywmwd	97	50	the	the	DT
cord-104279-choywmwd	97	51	2.7-kbp	2.7-kbp	CD
cord-104279-choywmwd	97	52	Sall	Sall	NNP
cord-104279-choywmwd	97	53	-	-	HYPH
cord-104279-choywmwd	97	54	HindlII	HindlII	NNP
cord-104279-choywmwd	97	55	fragment	fragment	NN
cord-104279-choywmwd	97	56	of	of	IN
cord-104279-choywmwd	97	57	DAP2	DAP2	NNP
cord-104279-choywmwd	97	58	(	(	-LRB-
cord-104279-choywmwd	97	59	Sail	sail	VB
cord-104279-choywmwd	97	60	blunt	blunt	JJ
cord-104279-choywmwd	97	61	ended	end	VBD
cord-104279-choywmwd	97	62	)	)	-RRB-
cord-104279-choywmwd	97	63	,	,	,
cord-104279-choywmwd	97	64	encoding	encode	VBG
cord-104279-choywmwd	97	65	the	the	DT
cord-104279-choywmwd	97	66	lumenal	lumenal	JJ
cord-104279-choywmwd	97	67	domain	domain	NN
cord-104279-choywmwd	97	68	of	of	IN
cord-104279-choywmwd	97	69	DPAP	DPAP	NNP
cord-104279-choywmwd	97	70	B	B	NNP
cord-104279-choywmwd	97	71	,	,	,
cord-104279-choywmwd	97	72	into	into	IN
cord-104279-choywmwd	97	73	the	the	DT
cord-104279-choywmwd	97	74	BamHI	BamHI	NNP
cord-104279-choywmwd	97	75	-	-	HYPH
cord-104279-choywmwd	97	76	HindlII	HindlII	NNP
cord-104279-choywmwd	97	77	sites	site	NNS
cord-104279-choywmwd	97	78	of	of	IN
cord-104279-choywmwd	97	79	pSEYC58	pseyc58	NN
cord-104279-choywmwd	97	80	.	.	.
cord-104279-choywmwd	98	1	The	the	DT
cord-104279-choywmwd	98	2	5.2-kbp	5.2-kbp	CD
cord-104279-choywmwd	98	3	BamHI	BamHI	NNP
cord-104279-choywmwd	98	4	-	-	HYPH
cord-104279-choywmwd	98	5	HindlII	HindlII	NNP
cord-104279-choywmwd	98	6	fragment	fragment	NN
cord-104279-choywmwd	98	7	of	of	IN
cord-104279-choywmwd	98	8	the	the	DT
cord-104279-choywmwd	98	9	resulting	result	VBG
cord-104279-choywmwd	98	10	vector	vector	NN
cord-104279-choywmwd	98	11	(	(	-LRB-
cord-104279-choywmwd	98	12	pCJR41	pCJR41	NNP
cord-104279-choywmwd	98	13	)	)	-RRB-
cord-104279-choywmwd	98	14	was	be	VBD
cord-104279-choywmwd	98	15	cloned	clone	VBN
cord-104279-choywmwd	98	16	into	into	IN
cord-104279-choywmwd	98	17	the	the	DT
cord-104279-choywmwd	98	18	BamHI	BamHI	NNP
cord-104279-choywmwd	98	19	-	-	HYPH
cord-104279-choywmwd	98	20	HindlII	HindlII	NNP
cord-104279-choywmwd	98	21	sites	site	NNS
cord-104279-choywmwd	98	22	of	of	IN
cord-104279-choywmwd	98	23	the	the	DT
cord-104279-choywmwd	98	24	2	2	CD
cord-104279-choywmwd	98	25	#	#	NN
cord-104279-choywmwd	98	26	plasmid	plasmid	NN
cord-104279-choywmwd	98	27	,	,	,
cord-104279-choywmwd	99	1	pSEY18	pSEY18	NNP
cord-104279-choywmwd	99	2	(	(	-LRB-
cord-104279-choywmwd	99	3	Emr	Emr	NNP
cord-104279-choywmwd	99	4	et	et	NNP
cord-104279-choywmwd	99	5	al	al	NNP
cord-104279-choywmwd	99	6	.	.	NNP
cord-104279-choywmwd	99	7	,	,	,
cord-104279-choywmwd	99	8	1986	1986	CD
cord-104279-choywmwd	99	9	)	)	-RRB-
cord-104279-choywmwd	99	10	.	.	.
cord-104279-choywmwd	100	1	The	the	DT
cord-104279-choywmwd	100	2	resulting	result	VBG
cord-104279-choywmwd	100	3	plasmid	plasmid	NN
cord-104279-choywmwd	100	4	encodes	encode	VBZ
cord-104279-choywmwd	100	5	AA	AA	NNP
cord-104279-choywmwd	100	6	-	-	HYPH
cord-104279-choywmwd	100	7	B	B	NNP
cord-104279-choywmwd	100	8	,	,	,
cord-104279-choywmwd	100	9	which	which	WDT
cord-104279-choywmwd	100	10	consists	consist	VBZ
cord-104279-choywmwd	100	11	of	of	IN
cord-104279-choywmwd	100	12	the	the	DT
cord-104279-choywmwd	100	13	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	100	14	150	150	CD
cord-104279-choywmwd	100	15	residues	residue	NNS
cord-104279-choywmwd	100	16	of	of	IN
cord-104279-choywmwd	100	17	DPAP	DPAP	NNP
cord-104279-choywmwd	100	18	A	A	NNP
cord-104279-choywmwd	100	19	to	to	TO
cord-104279-choywmwd	100	20	residue	residue	NN
cord-104279-choywmwd	100	21	47	47	CD
cord-104279-choywmwd	100	22	of	of	IN
cord-104279-choywmwd	100	23	DPAP	DPAP	NNP
cord-104279-choywmwd	100	24	B	B	NNP
cord-104279-choywmwd	100	25	(	(	-LRB-
cord-104279-choywmwd	100	26	Fig	Fig	NNP
cord-104279-choywmwd	100	27	.	.	.
cord-104279-choywmwd	100	28	1	1	CD
cord-104279-choywmwd	100	29	C	C	NNP
cord-104279-choywmwd	100	30	)	)	-RRB-
cord-104279-choywmwd	100	31	.	.	.
cord-104279-choywmwd	101	1	For	for	IN
cord-104279-choywmwd	101	2	the	the	DT
cord-104279-choywmwd	101	3	construction	construction	NN
cord-104279-choywmwd	101	4	of	of	IN
cord-104279-choywmwd	101	5	the	the	DT
cord-104279-choywmwd	101	6	fusion	fusion	NN
cord-104279-choywmwd	101	7	protein	protein	NN
cord-104279-choywmwd	101	8	A	A	NNP
cord-104279-choywmwd	101	9	-	-	HYPH
cord-104279-choywmwd	101	10	BB	bb	NN
cord-104279-choywmwd	101	11	,	,	,
cord-104279-choywmwd	101	12	XbaI	XbaI	NNP
cord-104279-choywmwd	101	13	sites	site	NNS
cord-104279-choywmwd	101	14	were	be	VBD
cord-104279-choywmwd	101	15	created	create	VBN
cord-104279-choywmwd	101	16	in	in	IN
cord-104279-choywmwd	101	17	both	both	CC
cord-104279-choywmwd	101	18	the	the	DT
cord-104279-choywmwd	101	19	DAP2	DAP2	NNP
cord-104279-choywmwd	101	20	and	and	CC
cord-104279-choywmwd	101	21	STE/3	STE/3	NNP
cord-104279-choywmwd	101	22	genes	gene	NNS
cord-104279-choywmwd	101	23	just	just	RB
cord-104279-choywmwd	101	24	upstream	upstream	RB
cord-104279-choywmwd	101	25	of	of	IN
cord-104279-choywmwd	101	26	the	the	DT
cord-104279-choywmwd	101	27	coding	code	VBG
cord-104279-choywmwd	101	28	regions	region	NNS
cord-104279-choywmwd	101	29	of	of	IN
cord-104279-choywmwd	101	30	the	the	DT
cord-104279-choywmwd	101	31	transmembrane	transmembrane	NN
cord-104279-choywmwd	101	32	domains	domain	NNS
cord-104279-choywmwd	101	33	of	of	IN
cord-104279-choywmwd	101	34	DPAP	DPAP	NNP
cord-104279-choywmwd	101	35	B	B	NNP
cord-104279-choywmwd	101	36	and	and	CC
cord-104279-choywmwd	101	37	DPAP	DPAP	NNP
cord-104279-choywmwd	101	38	A	A	NNP
cord-104279-choywmwd	101	39	,	,	,
cord-104279-choywmwd	101	40	respectively	respectively	RB
cord-104279-choywmwd	101	41	,	,	,
cord-104279-choywmwd	101	42	using	use	VBG
cord-104279-choywmwd	101	43	oligonucleotide	oligonucleotide	RB
cord-104279-choywmwd	101	44	-	-	HYPH
cord-104279-choywmwd	101	45	directed	direct	VBN
cord-104279-choywmwd	101	46	mutagenesis	mutagenesis	NN
cord-104279-choywmwd	101	47	(	(	-LRB-
cord-104279-choywmwd	101	48	Kunkel	Kunkel	NNP
cord-104279-choywmwd	101	49	et	et	NNP
cord-104279-choywmwd	101	50	al	al	NNP
cord-104279-choywmwd	101	51	.	.	NNP
cord-104279-choywmwd	101	52	,	,	,
cord-104279-choywmwd	101	53	1987	1987	CD
cord-104279-choywmwd	101	54	)	)	-RRB-
cord-104279-choywmwd	101	55	.	.	.
cord-104279-choywmwd	102	1	Nucleotides	nucleotide	NNS
cord-104279-choywmwd	102	2	69	69	CD
cord-104279-choywmwd	102	3	and	and	CC
cord-104279-choywmwd	102	4	70	70	CD
cord-104279-choywmwd	102	5	of	of	IN
cord-104279-choywmwd	102	6	the	the	DT
cord-104279-choywmwd	102	7	DAP2	DAP2	NNP
cord-104279-choywmwd	102	8	gene	gene	NN
cord-104279-choywmwd	102	9	were	be	VBD
cord-104279-choywmwd	102	10	changed	change	VBN
cord-104279-choywmwd	102	11	from	from	IN
cord-104279-choywmwd	102	12	GT	GT	NNP
cord-104279-choywmwd	102	13	to	to	IN
cord-104279-choywmwd	102	14	TC	TC	NNP
cord-104279-choywmwd	103	1	(	(	-LRB-
cord-104279-choywmwd	103	2	Roberts	Roberts	NNP
cord-104279-choywmwd	103	3	et	et	NNP
cord-104279-choywmwd	103	4	al	al	NNP
cord-104279-choywmwd	103	5	.	.	NNP
cord-104279-choywmwd	103	6	,	,	,
cord-104279-choywmwd	103	7	1989	1989	CD
cord-104279-choywmwd	103	8	)	)	-RRB-
cord-104279-choywmwd	103	9	,	,	,
cord-104279-choywmwd	103	10	and	and	CC
cord-104279-choywmwd	103	11	nucleotides	nucleotide	NNS
cord-104279-choywmwd	103	12	342	342	CD
cord-104279-choywmwd	103	13	-	-	SYM
cord-104279-choywmwd	103	14	344	344	CD
cord-104279-choywmwd	103	15	of	of	IN
cord-104279-choywmwd	103	16	STE/3	STE/3	NNP
cord-104279-choywmwd	103	17	were	be	VBD
cord-104279-choywmwd	103	18	changed	change	VBN
cord-104279-choywmwd	103	19	from	from	IN
cord-104279-choywmwd	103	20	GCC	GCC	NNP
cord-104279-choywmwd	103	21	to	to	IN
cord-104279-choywmwd	103	22	AGA	AGA	NNP
cord-104279-choywmwd	103	23	(	(	-LRB-
cord-104279-choywmwd	103	24	with	with	IN
cord-104279-choywmwd	103	25	the	the	DT
cord-104279-choywmwd	103	26	A	a	NN
cord-104279-choywmwd	103	27	of	of	IN
cord-104279-choywmwd	103	28	the	the	DT
cord-104279-choywmwd	103	29	initiation	initiation	NN
cord-104279-choywmwd	103	30	codon	codon	NN
cord-104279-choywmwd	103	31	as	as	IN
cord-104279-choywmwd	103	32	1	1	CD
cord-104279-choywmwd	103	33	)	)	-RRB-
cord-104279-choywmwd	104	1	(	(	-LRB-
cord-104279-choywmwd	104	2	C.	C.	NNP
cord-104279-choywmwd	104	3	A.	A.	NNP
cord-104279-choywmwd	104	4	Flanagan	Flanagan	NNP
cord-104279-choywmwd	104	5	,	,	,
cord-104279-choywmwd	104	6	D.	D.	NNP
cord-104279-choywmwd	104	7	A.	A.	NNP
cord-104279-choywmwd	104	8	Barnes	Barnes	NNP
cord-104279-choywmwd	104	9	,	,	,
cord-104279-choywmwd	104	10	M.	M.	NNP
cord-104279-choywmwd	104	11	C.	C.	NNP
cord-104279-choywmwd	104	12	Flessel	Flessel	NNP
cord-104279-choywmwd	104	13	,	,	,
cord-104279-choywmwd	104	14	and	and	CC
cord-104279-choywmwd	104	15	J.	J.	NNP
cord-104279-choywmwd	104	16	Thorner	Thorner	NNP
cord-104279-choywmwd	104	17	,	,	,
cord-104279-choywmwd	104	18	manuscript	manuscript	NN
cord-104279-choywmwd	104	19	submitted	submit	VBN
cord-104279-choywmwd	104	20	for	for	IN
cord-104279-choywmwd	104	21	publication	publication	NN
cord-104279-choywmwd	104	22	)	)	-RRB-
cord-104279-choywmwd	104	23	.	.	.
cord-104279-choywmwd	105	1	A	a	DT
cord-104279-choywmwd	105	2	1.1kbp	1.1kbp	CD
cord-104279-choywmwd	105	3	SacI	SacI	NNP
cord-104279-choywmwd	105	4	-	-	HYPH
cord-104279-choywmwd	105	5	XbaI	XbaI	NNP
cord-104279-choywmwd	105	6	fragment	fragment	NN
cord-104279-choywmwd	105	7	(	(	-LRB-
cord-104279-choywmwd	105	8	encoding	encode	VBG
cord-104279-choywmwd	105	9	the	the	DT
cord-104279-choywmwd	105	10	STE13	STE13	NNP
cord-104279-choywmwd	105	11	promoter	promoter	NN
cord-104279-choywmwd	105	12	and	and	CC
cord-104279-choywmwd	105	13	the	the	DT
cord-104279-choywmwd	105	14	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	105	15	domain	domain	NN
cord-104279-choywmwd	105	16	of	of	IN
cord-104279-choywmwd	105	17	DPAP	DPAP	NNP
cord-104279-choywmwd	105	18	A	A	NNP
cord-104279-choywmwd	105	19	)	)	-RRB-
cord-104279-choywmwd	105	20	and	and	CC
cord-104279-choywmwd	105	21	a	a	DT
cord-104279-choywmwd	105	22	2.8-kbp	2.8-kbp	CD
cord-104279-choywmwd	105	23	Xbal	Xbal	NNP
cord-104279-choywmwd	105	24	-	-	HYPH
cord-104279-choywmwd	105	25	HindlII	HindlII	NNP
cord-104279-choywmwd	105	26	DAP2	DAP2	NNP
cord-104279-choywmwd	105	27	fragment	fragment	NN
cord-104279-choywmwd	105	28	(	(	-LRB-
cord-104279-choywmwd	105	29	encoding	encode	VBG
cord-104279-choywmwd	105	30	the	the	DT
cord-104279-choywmwd	105	31	transmembrane	transmembrane	NN
cord-104279-choywmwd	105	32	and	and	CC
cord-104279-choywmwd	105	33	lumenal	lumenal	JJ
cord-104279-choywmwd	105	34	domains	domain	NNS
cord-104279-choywmwd	105	35	of	of	IN
cord-104279-choywmwd	105	36	DPAP	DPAP	NNP
cord-104279-choywmwd	105	37	B	B	NNP
cord-104279-choywmwd	105	38	)	)	-RRB-
cord-104279-choywmwd	105	39	were	be	VBD
cord-104279-choywmwd	105	40	ligated	ligate	VBN
cord-104279-choywmwd	105	41	into	into	IN
cord-104279-choywmwd	105	42	the	the	DT
cord-104279-choywmwd	105	43	SacI	SacI	NNP
cord-104279-choywmwd	105	44	-	-	HYPH
cord-104279-choywmwd	105	45	HindlII	HindlII	NNP
cord-104279-choywmwd	105	46	sites	site	NNS
cord-104279-choywmwd	105	47	of	of	IN
cord-104279-choywmwd	105	48	pSEY18	pSEY18	NNP
cord-104279-choywmwd	105	49	.	.	.
cord-104279-choywmwd	106	1	The	the	DT
cord-104279-choywmwd	106	2	resulting	result	VBG
cord-104279-choywmwd	106	3	plasmid	plasmid	NN
cord-104279-choywmwd	106	4	(	(	-LRB-
cord-104279-choywmwd	106	5	pCJR67	pCJR67	NNP
cord-104279-choywmwd	106	6	)	)	-RRB-
cord-104279-choywmwd	106	7	encodes	encode	VBZ
cord-104279-choywmwd	106	8	a	a	DT
cord-104279-choywmwd	106	9	protein	protein	NN
cord-104279-choywmwd	106	10	consisting	consist	VBG
cord-104279-choywmwd	106	11	of	of	IN
cord-104279-choywmwd	106	12	113	113	CD
cord-104279-choywmwd	106	13	residues	residue	NNS
cord-104279-choywmwd	106	14	of	of	IN
cord-104279-choywmwd	106	15	the	the	DT
cord-104279-choywmwd	106	16	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	106	17	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	106	18	domain	domain	NN
cord-104279-choywmwd	106	19	of	of	IN
cord-104279-choywmwd	106	20	DPAP	DPAP	NNP
cord-104279-choywmwd	106	21	A	A	NNP
cord-104279-choywmwd	106	22	fused	fuse	VBN
cord-104279-choywmwd	106	23	to	to	IN
cord-104279-choywmwd	106	24	amino	amino	NN
cord-104279-choywmwd	106	25	acid	acid	NN
cord-104279-choywmwd	106	26	24	24	CD
cord-104279-choywmwd	106	27	of	of	IN
cord-104279-choywmwd	106	28	DPAP	DPAP	NNP
cord-104279-choywmwd	106	29	B	B	NNP
cord-104279-choywmwd	106	30	(	(	-LRB-
cord-104279-choywmwd	106	31	Fig	Fig	NNP
cord-104279-choywmwd	106	32	.	.	.
cord-104279-choywmwd	106	33	1	1	CD
cord-104279-choywmwd	106	34	C	C	NNP
cord-104279-choywmwd	106	35	)	)	-RRB-
cord-104279-choywmwd	106	36	.	.	.
cord-104279-choywmwd	107	1	The	the	DT
cord-104279-choywmwd	107	2	fusion	fusion	NN
cord-104279-choywmwd	107	3	protein	protein	NN
cord-104279-choywmwd	107	4	B	B	NNP
cord-104279-choywmwd	107	5	-	-	HYPH
cord-104279-choywmwd	107	6	A	A	NNP
cord-104279-choywmwd	107	7	-	-	HYPH
cord-104279-choywmwd	107	8	B	B	NNP
cord-104279-choywmwd	107	9	was	be	VBD
cord-104279-choywmwd	107	10	constructed	construct	VBN
cord-104279-choywmwd	107	11	as	as	IN
cord-104279-choywmwd	107	12	follows	follow	VBZ
cord-104279-choywmwd	107	13	:	:	:
cord-104279-choywmwd	107	14	the	the	DT
cord-104279-choywmwd	107	15	3.3-kbp	3.3-kbp	CD
cord-104279-choywmwd	107	16	BamHI	BamHI	NNP
cord-104279-choywmwd	107	17	-	-	HYPH
cord-104279-choywmwd	107	18	HindUI	HindUI	NNP
cord-104279-choywmwd	107	19	DAP2	DAP2	NNP
cord-104279-choywmwd	107	20	fragment	fragment	NN
cord-104279-choywmwd	107	21	,	,	,
cord-104279-choywmwd	107	22	including	include	VBG
cord-104279-choywmwd	107	23	the	the	DT
cord-104279-choywmwd	107	24	Xbal	Xbal	NNP
cord-104279-choywmwd	107	25	site	site	NN
cord-104279-choywmwd	107	26	at	at	IN
cord-104279-choywmwd	107	27	+70	+70	CD
cord-104279-choywmwd	107	28	,	,	,
cord-104279-choywmwd	107	29	was	be	VBD
cord-104279-choywmwd	107	30	ligated	ligate	VBN
cord-104279-choywmwd	107	31	into	into	IN
cord-104279-choywmwd	107	32	the	the	DT
cord-104279-choywmwd	107	33	BamHI	BamHI	NNP
cord-104279-choywmwd	107	34	-	-	HYPH
cord-104279-choywmwd	107	35	HindlII	HindlII	NNP
cord-104279-choywmwd	107	36	sites	site	NNS
cord-104279-choywmwd	107	37	of	of	IN
cord-104279-choywmwd	107	38	pSEYC58	pseyc58	NN
cord-104279-choywmwd	107	39	.	.	.
cord-104279-choywmwd	108	1	A	a	DT
cord-104279-choywmwd	108	2	4.3-kbp	4.3-kbp	CD
cord-104279-choywmwd	108	3	Xbal	Xbal	NNP
cord-104279-choywmwd	108	4	-	-	HYPH
cord-104279-choywmwd	108	5	HindlII	HindlII	NNP
cord-104279-choywmwd	108	6	fragment	fragment	NN
cord-104279-choywmwd	108	7	of	of	IN
cord-104279-choywmwd	108	8	STE/3	STE/3	NNP
cord-104279-choywmwd	108	9	,	,	,
cord-104279-choywmwd	108	10	encoding	encode	VBG
cord-104279-choywmwd	108	11	the	the	DT
cord-104279-choywmwd	108	12	transmembrane	transmembrane	NN
cord-104279-choywmwd	108	13	and	and	CC
cord-104279-choywmwd	108	14	lumenal	lumenal	JJ
cord-104279-choywmwd	108	15	domains	domain	NNS
cord-104279-choywmwd	108	16	of	of	IN
cord-104279-choywmwd	108	17	DPAP	DPAP	NNP
cord-104279-choywmwd	108	18	A	A	NNP
cord-104279-choywmwd	108	19	,	,	,
cord-104279-choywmwd	108	20	was	be	VBD
cord-104279-choywmwd	108	21	inserted	insert	VBN
cord-104279-choywmwd	108	22	into	into	IN
cord-104279-choywmwd	108	23	the	the	DT
cord-104279-choywmwd	108	24	XbaI	XbaI	NNP
cord-104279-choywmwd	108	25	-	-	HYPH
cord-104279-choywmwd	108	26	HindlII	HindlII	NNP
cord-104279-choywmwd	108	27	sites	site	NNS
cord-104279-choywmwd	108	28	,	,	,
cord-104279-choywmwd	108	29	resulting	result	VBG
cord-104279-choywmwd	108	30	in	in	IN
cord-104279-choywmwd	108	31	the	the	DT
cord-104279-choywmwd	108	32	plasmid	plasmid	NN
cord-104279-choywmwd	108	33	pC	pc	NN
cord-104279-choywmwd	108	34	JR58	JR58	NNP
cord-104279-choywmwd	108	35	.	.	.
cord-104279-choywmwd	109	1	The	the	DT
cord-104279-choywmwd	109	2	2.7-kbp	2.7-kbp	CD
cord-104279-choywmwd	109	3	SalI	SalI	NNP
cord-104279-choywmwd	109	4	-	-	HYPH
cord-104279-choywmwd	109	5	HindIII	HindIII	NNP
cord-104279-choywmwd	109	6	DAP2	DAP2	NNP
cord-104279-choywmwd	109	7	fragment	fragment	NN
cord-104279-choywmwd	109	8	(	(	-LRB-
cord-104279-choywmwd	109	9	SalI	SalI	NNP
cord-104279-choywmwd	109	10	blunt	blunt	JJ
cord-104279-choywmwd	109	11	ended	end	VBD
cord-104279-choywmwd	109	12	)	)	-RRB-
cord-104279-choywmwd	109	13	,	,	,
cord-104279-choywmwd	109	14	encoding	encode	VBG
cord-104279-choywmwd	109	15	the	the	DT
cord-104279-choywmwd	109	16	DPAP	DPAP	NNP
cord-104279-choywmwd	109	17	B	B	NNP
cord-104279-choywmwd	109	18	lumenal	lumenal	JJ
cord-104279-choywmwd	109	19	domain	domain	NN
cord-104279-choywmwd	109	20	,	,	,
cord-104279-choywmwd	109	21	was	be	VBD
cord-104279-choywmwd	109	22	inserted	insert	VBN
cord-104279-choywmwd	109	23	into	into	IN
cord-104279-choywmwd	109	24	the	the	DT
cord-104279-choywmwd	109	25	MluI	MluI	NNP
cord-104279-choywmwd	109	26	-	-	HYPH
cord-104279-choywmwd	109	27	HindlII	HindlII	NNP
cord-104279-choywmwd	109	28	sites	site	NNS
cord-104279-choywmwd	109	29	(	(	-LRB-
cord-104279-choywmwd	109	30	MluI	MluI	NNP
cord-104279-choywmwd	109	31	blunt	blunt	NNP
cord-104279-choywmwd	109	32	ended	end	VBD
cord-104279-choywmwd	109	33	)	)	-RRB-
cord-104279-choywmwd	109	34	of	of	IN
cord-104279-choywmwd	109	35	pCJR58	pCJR58	NNP
cord-104279-choywmwd	109	36	,	,	,
cord-104279-choywmwd	109	37	resulting	result	VBG
cord-104279-choywmwd	109	38	in	in	IN
cord-104279-choywmwd	109	39	pCJR69	pCJR69	NNP
cord-104279-choywmwd	109	40	,	,	,
cord-104279-choywmwd	109	41	a	a	DT
cord-104279-choywmwd	109	42	CEN	CEN	NNP
cord-104279-choywmwd	109	43	plasmid	plasmid	NN
cord-104279-choywmwd	109	44	encoding	encoding	NN
cord-104279-choywmwd	109	45	B	b	NN
cord-104279-choywmwd	109	46	-	-	HYPH
cord-104279-choywmwd	109	47	A	A	NNP
cord-104279-choywmwd	109	48	-	-	HYPH
cord-104279-choywmwd	109	49	B.	b.	NN
cord-104279-choywmwd	110	1	The	the	DT
cord-104279-choywmwd	110	2	4.8-kbp	4.8-kbp	CD
cord-104279-choywmwd	110	3	BamHI	BamHI	NNP
cord-104279-choywmwd	110	4	-	-	HYPH
cord-104279-choywmwd	110	5	HindlII	HindlII	NNP
cord-104279-choywmwd	110	6	fragment	fragment	NN
cord-104279-choywmwd	110	7	from	from	IN
cord-104279-choywmwd	110	8	pCJR69	pCJR69	NNP
cord-104279-choywmwd	110	9	was	be	VBD
cord-104279-choywmwd	110	10	inserted	insert	VBN
cord-104279-choywmwd	110	11	into	into	IN
cord-104279-choywmwd	110	12	the	the	DT
cord-104279-choywmwd	110	13	same	same	JJ
cord-104279-choywmwd	110	14	sites	site	NNS
cord-104279-choywmwd	110	15	of	of	IN
cord-104279-choywmwd	110	16	pSEY18	pSEY18	NNP
cord-104279-choywmwd	110	17	,	,	,
cord-104279-choywmwd	110	18	resulting	result	VBG
cord-104279-choywmwd	110	19	in	in	IN
cord-104279-choywmwd	110	20	pCJRT0	pCJRT0	NNP
cord-104279-choywmwd	110	21	.	.	.
cord-104279-choywmwd	111	1	B	b	LS
cord-104279-choywmwd	111	2	-	-	HYPH
cord-104279-choywmwd	111	3	A	a	NN
cord-104279-choywmwd	111	4	-	-	HYPH
cord-104279-choywmwd	111	5	B	b	NN
cord-104279-choywmwd	111	6	was	be	VBD
cord-104279-choywmwd	111	7	placed	place	VBN
cord-104279-choywmwd	111	8	under	under	IN
cord-104279-choywmwd	111	9	the	the	DT
cord-104279-choywmwd	111	10	control	control	NN
cord-104279-choywmwd	111	11	of	of	IN
cord-104279-choywmwd	111	12	the	the	DT
cord-104279-choywmwd	111	13	GAL	GAL	NNP
cord-104279-choywmwd	111	14	/	/	SYM
cord-104279-choywmwd	111	15	promoter	promoter	NN
cord-104279-choywmwd	111	16	by	by	IN
cord-104279-choywmwd	111	17	cloning	clone	VBG
cord-104279-choywmwd	111	18	the	the	DT
cord-104279-choywmwd	111	19	NheI	NheI	NNP
cord-104279-choywmwd	111	20	-	-	HYPH
cord-104279-choywmwd	111	21	HindlII	HindlII	NNP
cord-104279-choywmwd	111	22	B	B	NNP
cord-104279-choywmwd	111	23	-	-	HYPH
cord-104279-choywmwd	111	24	A	A	NNP
cord-104279-choywmwd	111	25	-	-	HYPH
cord-104279-choywmwd	111	26	B	b	NN
cord-104279-choywmwd	111	27	fragment	fragment	NN
cord-104279-choywmwd	111	28	into	into	IN
cord-104279-choywmwd	111	29	the	the	DT
cord-104279-choywmwd	111	30	XbaI	XbaI	NNP
cord-104279-choywmwd	111	31	-	-	HYPH
cord-104279-choywmwd	111	32	HindlII	HindlII	NNP
cord-104279-choywmwd	111	33	sites	site	NNS
cord-104279-choywmwd	111	34	of	of	IN
cord-104279-choywmwd	111	35	pCJR52	pCJR52	NNP
cord-104279-choywmwd	111	36	,	,	,
cord-104279-choywmwd	111	37	creating	create	VBG
cord-104279-choywmwd	111	38	pC	pc	NN
cord-104279-choywmwd	112	1	JR79	JR79	NNP
cord-104279-choywmwd	112	2	.	.	.
cord-104279-choywmwd	113	1	The	the	DT
cord-104279-choywmwd	113	2	B	B	NNP
cord-104279-choywmwd	113	3	-	-	HYPH
cord-104279-choywmwd	113	4	A	a	NN
cord-104279-choywmwd	113	5	-	-	HYPH
cord-104279-choywmwd	113	6	B	b	NN
cord-104279-choywmwd	113	7	fusion	fusion	NN
cord-104279-choywmwd	113	8	protein	protein	NN
cord-104279-choywmwd	113	9	consists	consist	VBZ
cord-104279-choywmwd	113	10	of	of	IN
cord-104279-choywmwd	113	11	residues	residue	NNS
cord-104279-choywmwd	113	12	116	116	CD
cord-104279-choywmwd	113	13	-	-	SYM
cord-104279-choywmwd	113	14	150	150	CD
cord-104279-choywmwd	113	15	of	of	IN
cord-104279-choywmwd	113	16	DPAP	DPAP	NNP
cord-104279-choywmwd	113	17	A	A	NNP
cord-104279-choywmwd	113	18	in	in	IN
cord-104279-choywmwd	113	19	place	place	NN
cord-104279-choywmwd	113	20	of	of	IN
cord-104279-choywmwd	113	21	residues	residue	NNS
cord-104279-choywmwd	113	22	26	26	CD
cord-104279-choywmwd	113	23	-	-	SYM
cord-104279-choywmwd	113	24	46	46	CD
cord-104279-choywmwd	113	25	of	of	IN
cord-104279-choywmwd	113	26	DPAP	DPAP	NNP
cord-104279-choywmwd	113	27	B	B	NNP
cord-104279-choywmwd	113	28	(	(	-LRB-
cord-104279-choywmwd	113	29	Fig	Fig	NNP
cord-104279-choywmwd	113	30	.	.	.
cord-104279-choywmwd	113	31	1	1	CD
cord-104279-choywmwd	113	32	C	C	NNP
cord-104279-choywmwd	113	33	)	)	-RRB-
cord-104279-choywmwd	113	34	.	.	.
cord-104279-choywmwd	114	1	Oligonucleotide	Oligonucleotide	NNP
cord-104279-choywmwd	114	2	-	-	HYPH
cord-104279-choywmwd	114	3	directed	direct	VBN
cord-104279-choywmwd	114	4	mutagenesis	mutagenesis	NN
cord-104279-choywmwd	114	5	of	of	IN
cord-104279-choywmwd	114	6	the	the	DT
cord-104279-choywmwd	114	7	portion	portion	NN
cord-104279-choywmwd	114	8	of	of	IN
cord-104279-choywmwd	114	9	STE/3	STE/3	NNP
cord-104279-choywmwd	114	10	encoding	encode	VBG
cord-104279-choywmwd	114	11	the	the	DT
cord-104279-choywmwd	114	12	118	118	CD
cord-104279-choywmwd	114	13	amino	amino	NN
cord-104279-choywmwd	114	14	acid	acid	NN
cord-104279-choywmwd	114	15	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	114	16	domain	domain	NN
cord-104279-choywmwd	114	17	of	of	IN
cord-104279-choywmwd	114	18	DPAP	DPAP	NNP
cord-104279-choywmwd	114	19	A	A	NNP
cord-104279-choywmwd	114	20	(	(	-LRB-
cord-104279-choywmwd	114	21	Fig	fig	NN
cord-104279-choywmwd	114	22	.	.	.
cord-104279-choywmwd	114	23	1	1	CD
cord-104279-choywmwd	114	24	A	a	CD
cord-104279-choywmwd	114	25	)	)	-RRB-
cord-104279-choywmwd	114	26	was	be	VBD
cord-104279-choywmwd	114	27	performed	perform	VBN
cord-104279-choywmwd	114	28	in	in	IN
cord-104279-choywmwd	114	29	pCJR71	pCJR71	NNP
cord-104279-choywmwd	114	30	,	,	,
cord-104279-choywmwd	114	31	which	which	WDT
cord-104279-choywmwd	114	32	consists	consist	VBZ
cord-104279-choywmwd	114	33	of	of	IN
cord-104279-choywmwd	114	34	the	the	DT
cord-104279-choywmwd	114	35	0.65-kbp	0.65-kbp	CD
cord-104279-choywmwd	114	36	EagI	EagI	NNP
cord-104279-choywmwd	114	37	-	-	HYPH
cord-104279-choywmwd	114	38	PstI	PstI	NNP
cord-104279-choywmwd	114	39	fragment	fragment	NN
cord-104279-choywmwd	114	40	of	of	IN
cord-104279-choywmwd	114	41	STE/3	STE/3	NNP
cord-104279-choywmwd	114	42	inserted	insert	VBD
cord-104279-choywmwd	114	43	into	into	IN
cord-104279-choywmwd	114	44	the	the	DT
cord-104279-choywmwd	114	45	EagI	EagI	NNP
cord-104279-choywmwd	114	46	-	-	HYPH
cord-104279-choywmwd	114	47	PstI	PstI	NNP
cord-104279-choywmwd	114	48	sites	site	NNS
cord-104279-choywmwd	114	49	of	of	IN
cord-104279-choywmwd	114	50	KS	KS	NNP
cord-104279-choywmwd	114	51	+	+	CC
cord-104279-choywmwd	114	52	.	.	.
cord-104279-choywmwd	115	1	A	a	DT
cord-104279-choywmwd	115	2	66-bp	66-bp	CD
cord-104279-choywmwd	115	3	in	in	IN
cord-104279-choywmwd	115	4	-	-	HYPH
cord-104279-choywmwd	115	5	frame	frame	NN
cord-104279-choywmwd	115	6	deletion	deletion	NN
cord-104279-choywmwd	115	7	,	,	,
cord-104279-choywmwd	115	8	removing	remove	VBG
cord-104279-choywmwd	115	9	the	the	DT
cord-104279-choywmwd	115	10	amino	amino	NN
cord-104279-choywmwd	115	11	acids	acid	NNS
cord-104279-choywmwd	115	12	85	85	CD
cord-104279-choywmwd	115	13	-	-	SYM
cord-104279-choywmwd	115	14	106	106	CD
cord-104279-choywmwd	115	15	(	(	-LRB-
cord-104279-choywmwd	115	16	z~22	z~22	NN
cord-104279-choywmwd	115	17	)	)	-RRB-
cord-104279-choywmwd	115	18	,	,	,
cord-104279-choywmwd	115	19	was	be	VBD
cord-104279-choywmwd	115	20	created	create	VBN
cord-104279-choywmwd	115	21	,	,	,
cord-104279-choywmwd	115	22	and	and	CC
cord-104279-choywmwd	115	23	the	the	DT
cord-104279-choywmwd	115	24	Sacl	Sacl	NNP
cord-104279-choywmwd	115	25	-	-	HYPH
cord-104279-choywmwd	115	26	MluI	MluI	NNP
cord-104279-choywmwd	115	27	fragment	fragment	NN
cord-104279-choywmwd	115	28	from	from	IN
cord-104279-choywmwd	115	29	this	this	DT
cord-104279-choywmwd	115	30	plasmid	plasmid	NN
cord-104279-choywmwd	115	31	was	be	VBD
cord-104279-choywmwd	115	32	inserted	insert	VBN
cord-104279-choywmwd	115	33	into	into	IN
cord-104279-choywmwd	115	34	the	the	DT
cord-104279-choywmwd	115	35	SacI	SacI	NNP
cord-104279-choywmwd	115	36	-	-	HYPH
cord-104279-choywmwd	115	37	MluI	MluI	NNP
cord-104279-choywmwd	115	38	sites	site	NNS
cord-104279-choywmwd	115	39	of	of	IN
cord-104279-choywmwd	115	40	pCJR64	pCJR64	NNP
cord-104279-choywmwd	115	41	,	,	,
cord-104279-choywmwd	115	42	creating	create	VBG
cord-104279-choywmwd	115	43	the	the	DT
cord-104279-choywmwd	115	44	2	2	CD
cord-104279-choywmwd	115	45	#	#	NN
cord-104279-choywmwd	115	46	plasmid	plasmid	NN
cord-104279-choywmwd	115	47	pSN58	psn58	NN
cord-104279-choywmwd	115	48	,	,	,
cord-104279-choywmwd	115	49	which	which	WDT
cord-104279-choywmwd	115	50	encodes	encode	VBZ
cord-104279-choywmwd	115	51	A92-AAB	a92-aab	CD
cord-104279-choywmwd	115	52	(	(	-LRB-
cord-104279-choywmwd	115	53	Fig	Fig	NNP
cord-104279-choywmwd	115	54	.	.	.
cord-104279-choywmwd	115	55	 	 	_SP
cord-104279-choywmwd	115	56	1	1	CD
cord-104279-choywmwd	115	57	C	C	NNP
cord-104279-choywmwd	115	58	)	)	-RRB-
cord-104279-choywmwd	115	59	.	.	.
cord-104279-choywmwd	116	1	The	the	DT
cord-104279-choywmwd	116	2	same	same	JJ
cord-104279-choywmwd	116	3	SacI	SacI	NNP
cord-104279-choywmwd	116	4	-	-	HYPH
cord-104279-choywmwd	116	5	MluI	MluI	NNP
cord-104279-choywmwd	116	6	fragment	fragment	NN
cord-104279-choywmwd	116	7	was	be	VBD
cord-104279-choywmwd	116	8	ligated	ligate	VBN
cord-104279-choywmwd	116	9	with	with	IN
cord-104279-choywmwd	116	10	the	the	DT
cord-104279-choywmwd	116	11	4-kbp	4-kbp	CD
cord-104279-choywmwd	116	12	MluI	MluI	NNP
cord-104279-choywmwd	116	13	-	-	HYPH
cord-104279-choywmwd	116	14	Hindlll	Hindlll	NNP
cord-104279-choywmwd	116	15	(	(	-LRB-
cord-104279-choywmwd	116	16	encoding	encode	VBG
cord-104279-choywmwd	116	17	the	the	DT
cord-104279-choywmwd	116	18	lumenal	lumenal	JJ
cord-104279-choywmwd	116	19	domain	domain	NN
cord-104279-choywmwd	116	20	of	of	IN
cord-104279-choywmwd	116	21	DPAP	DPAP	NNP
cord-104279-choywmwd	116	22	A	A	NNP
cord-104279-choywmwd	116	23	)	)	-RRB-
cord-104279-choywmwd	116	24	into	into	IN
cord-104279-choywmwd	116	25	the	the	DT
cord-104279-choywmwd	116	26	SacI	SacI	NNP
cord-104279-choywmwd	116	27	-	-	HYPH
cord-104279-choywmwd	116	28	HindIII	HindIII	NNP
cord-104279-choywmwd	116	29	sites	site	NNS
cord-104279-choywmwd	116	30	of	of	IN
cord-104279-choywmwd	116	31	pSEY18	pSEY18	NNP
cord-104279-choywmwd	116	32	,	,	,
cord-104279-choywmwd	116	33	creating	create	VBG
cord-104279-choywmwd	116	34	pSN59	pSN59	NNS
cord-104279-choywmwd	116	35	,	,	,
cord-104279-choywmwd	116	36	which	which	WDT
cord-104279-choywmwd	116	37	encodes	encode	VBZ
cord-104279-choywmwd	116	38	A22-AAA	a22-aaa	CD
cord-104279-choywmwd	116	39	(	(	-LRB-
cord-104279-choywmwd	116	40	Fig	fig	NN
cord-104279-choywmwd	116	41	.	.	.
cord-104279-choywmwd	116	42	1	1	CD
cord-104279-choywmwd	116	43	C	C	NNP
cord-104279-choywmwd	116	44	)	)	-RRB-
cord-104279-choywmwd	116	45	.	.	.
cord-104279-choywmwd	117	1	A22-AA	a22-aa	NN
cord-104279-choywmwd	117	2	-	-	HYPH
cord-104279-choywmwd	117	3	B	B	NNP
cord-104279-choywmwd	117	4	was	be	VBD
cord-104279-choywmwd	117	5	placed	place	VBN
cord-104279-choywmwd	117	6	under	under	IN
cord-104279-choywmwd	117	7	the	the	DT
cord-104279-choywmwd	117	8	control	control	NN
cord-104279-choywmwd	117	9	of	of	IN
cord-104279-choywmwd	117	10	the	the	DT
cord-104279-choywmwd	117	11	GAL	GAL	NNP
cord-104279-choywmwd	117	12	/	/	SYM
cord-104279-choywmwd	117	13	promoter	promoter	NN
cord-104279-choywmwd	117	14	by	by	IN
cord-104279-choywmwd	117	15	fusing	fuse	VBG
cord-104279-choywmwd	117	16	a	a	DT
cord-104279-choywmwd	117	17	SacI	SacI	NNP
cord-104279-choywmwd	117	18	-	-	HYPH
cord-104279-choywmwd	117	19	EagI	EagI	NNP
cord-104279-choywmwd	117	20	fragment	fragment	NN
cord-104279-choywmwd	117	21	from	from	IN
cord-104279-choywmwd	117	22	pCJR125	pCJR125	NNP
cord-104279-choywmwd	118	1	(	(	-LRB-
cord-104279-choywmwd	118	2	contains	contain	VBZ
cord-104279-choywmwd	118	3	the	the	DT
cord-104279-choywmwd	118	4	360	360	CD
cord-104279-choywmwd	118	5	bp	bp	NNP
cord-104279-choywmwd	118	6	HindlII	HindlII	NNP
cord-104279-choywmwd	118	7	-	-	HYPH
cord-104279-choywmwd	118	8	EcoRl	EcoRl	NNP
cord-104279-choywmwd	118	9	GAL	GAL	NNP
cord-104279-choywmwd	118	10	/	/	SYM
cord-104279-choywmwd	118	11	fragment	fragment	NN
cord-104279-choywmwd	118	12	in	in	IN
cord-104279-choywmwd	118	13	pGEM-5Zf	pgem-5zf	ADD
cord-104279-choywmwd	118	14	)	)	-RRB-
cord-104279-choywmwd	118	15	to	to	IN
cord-104279-choywmwd	118	16	an	an	DT
cord-104279-choywmwd	118	17	Eagl	Eagl	NNP
cord-104279-choywmwd	118	18	-	-	HYPH
cord-104279-choywmwd	118	19	HindlII	HindlII	NNP
cord-104279-choywmwd	118	20	fragment	fragment	NN
cord-104279-choywmwd	118	21	from	from	IN
cord-104279-choywmwd	118	22	pSN58	pSN58	NNP
cord-104279-choywmwd	118	23	,	,	,
cord-104279-choywmwd	118	24	both	both	DT
cord-104279-choywmwd	118	25	of	of	IN
cord-104279-choywmwd	118	26	which	which	WDT
cord-104279-choywmwd	118	27	were	be	VBD
cord-104279-choywmwd	118	28	ligated	ligate	VBN
cord-104279-choywmwd	118	29	into	into	IN
cord-104279-choywmwd	118	30	the	the	DT
cord-104279-choywmwd	118	31	SacI	SacI	NNP
cord-104279-choywmwd	118	32	-	-	HYPH
cord-104279-choywmwd	118	33	HindlII	HindlII	NNP
cord-104279-choywmwd	118	34	sites	site	NNS
cord-104279-choywmwd	118	35	of	of	IN
cord-104279-choywmwd	118	36	pSEY18	pSEY18	NNP
cord-104279-choywmwd	118	37	,	,	,
cord-104279-choywmwd	118	38	creating	create	VBG
cord-104279-choywmwd	118	39	pSN89	pSN89	NNS
cord-104279-choywmwd	118	40	.	.	.
cord-104279-choywmwd	119	1	The	the	DT
cord-104279-choywmwd	119	2	Fusl	Fusl	NNP
cord-104279-choywmwd	119	3	-	-	HYPH
cord-104279-choywmwd	119	4	LacZp	laczp	JJ
cord-104279-choywmwd	119	5	expression	expression	NN
cord-104279-choywmwd	119	6	plasmid	plasmid	NN
cord-104279-choywmwd	119	7	pCJRll4	pcjrll4	NN
cord-104279-choywmwd	119	8	as	as	IN
cord-104279-choywmwd	119	9	follows	follow	VBZ
cord-104279-choywmwd	119	10	:	:	:
cord-104279-choywmwd	120	1	a	a	DT
cord-104279-choywmwd	120	2	6-kb	6-kb	CD
cord-104279-choywmwd	120	3	NheI	NheI	NNP
cord-104279-choywmwd	120	4	-	-	HYPH
cord-104279-choywmwd	120	5	HindllI	hindlli	JJ
cord-104279-choywmwd	120	6	fragment	fragment	NN
cord-104279-choywmwd	120	7	,	,	,
cord-104279-choywmwd	120	8	encoding	encode	VBG
cord-104279-choywmwd	120	9	the	the	DT
cord-104279-choywmwd	120	10	254	254	CD
cord-104279-choywmwd	120	11	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	120	12	amino	amino	JJ
cord-104279-choywmwd	120	13	acids	acid	NNS
cord-104279-choywmwd	120	14	of	of	IN
cord-104279-choywmwd	120	15	the	the	DT
cord-104279-choywmwd	120	16	FUS1	FUS1	NNP
cord-104279-choywmwd	120	17	protein	protein	NN
cord-104279-choywmwd	120	18	fused	fuse	VBN
cord-104279-choywmwd	120	19	to	to	IN
cord-104279-choywmwd	120	20	~-galactosidase	~-galactosidase	NNP
cord-104279-choywmwd	120	21	,	,	,
cord-104279-choywmwd	120	22	under	under	IN
cord-104279-choywmwd	120	23	the	the	DT
cord-104279-choywmwd	120	24	GALl	gall	NN
cord-104279-choywmwd	120	25	promoter	promoter	NN
cord-104279-choywmwd	120	26	(	(	-LRB-
cord-104279-choywmwd	120	27	isolated	isolate	VBN
cord-104279-choywmwd	120	28	from	from	IN
cord-104279-choywmwd	120	29	pCJRll3	pCJRll3	NNP
cord-104279-choywmwd	120	30	,	,	,
cord-104279-choywmwd	120	31	a	a	DT
cord-104279-choywmwd	120	32	derivative	derivative	NN
cord-104279-choywmwd	120	33	of	of	IN
cord-104279-choywmwd	120	34	pSB231	pSB231	NNP
cord-104279-choywmwd	120	35	(	(	-LRB-
cord-104279-choywmwd	120	36	Trueheart	Trueheart	NNP
cord-104279-choywmwd	120	37	and	and	CC
cord-104279-choywmwd	120	38	Fink	Fink	NNP
cord-104279-choywmwd	120	39	,	,	,
cord-104279-choywmwd	120	40	1989	1989	CD
cord-104279-choywmwd	120	41	)	)	-RRB-
cord-104279-choywmwd	120	42	)	)	-RRB-
cord-104279-choywmwd	120	43	was	be	VBD
cord-104279-choywmwd	120	44	cloned	clone	VBN
cord-104279-choywmwd	120	45	into	into	IN
cord-104279-choywmwd	120	46	the	the	DT
cord-104279-choywmwd	120	47	Xbal	Xbal	NNP
cord-104279-choywmwd	120	48	-	-	HYPH
cord-104279-choywmwd	120	49	Hindlll	Hindlll	NNP
cord-104279-choywmwd	120	50	sites	site	NNS
cord-104279-choywmwd	120	51	of	of	IN
cord-104279-choywmwd	120	52	pVT105U	pVT105U	NNP
cord-104279-choywmwd	120	53	,	,	,
cord-104279-choywmwd	120	54	a	a	DT
cord-104279-choywmwd	120	55	2	2	CD
cord-104279-choywmwd	120	56	#	#	NN
cord-104279-choywmwd	120	57	plasmid	plasmid	NN
cord-104279-choywmwd	120	58	(	(	-LRB-
cord-104279-choywmwd	120	59	Vernet	Vernet	NNP
cord-104279-choywmwd	120	60	et	et	NNP
cord-104279-choywmwd	120	61	al	al	NNP
cord-104279-choywmwd	120	62	.	.	NNP
cord-104279-choywmwd	120	63	,	,	,
cord-104279-choywmwd	120	64	1987	1987	CD
cord-104279-choywmwd	120	65	)	)	-RRB-
cord-104279-choywmwd	120	66	,	,	,
cord-104279-choywmwd	120	67	creating	create	VBG
cord-104279-choywmwd	120	68	pCJR114	pCJR114	NNP
cord-104279-choywmwd	120	69	.	.	.
cord-104279-choywmwd	121	1	For	for	IN
cord-104279-choywmwd	121	2	the	the	DT
cord-104279-choywmwd	121	3	production	production	NN
cord-104279-choywmwd	121	4	of	of	IN
cord-104279-choywmwd	121	5	DPAP	DPAP	NNP
cord-104279-choywmwd	121	6	A	a	DT
cord-104279-choywmwd	121	7	antigen	antigen	NN
cord-104279-choywmwd	121	8	to	to	TO
cord-104279-choywmwd	121	9	be	be	VB
cord-104279-choywmwd	121	10	used	use	VBN
cord-104279-choywmwd	121	11	to	to	TO
cord-104279-choywmwd	121	12	generate	generate	VB
cord-104279-choywmwd	121	13	DPAP	DPAP	NNP
cord-104279-choywmwd	121	14	A	a	DT
cord-104279-choywmwd	121	15	antiserum	antiserum	NN
cord-104279-choywmwd	121	16	,	,	,
cord-104279-choywmwd	121	17	the	the	DT
cord-104279-choywmwd	121	18	plasmid	plasmid	NN
cord-104279-choywmwd	121	19	pCJR24	pCJR24	NNP
cord-104279-choywmwd	121	20	was	be	VBD
cord-104279-choywmwd	121	21	constructed	construct	VBN
cord-104279-choywmwd	121	22	by	by	IN
cord-104279-choywmwd	121	23	inserting	insert	VBG
cord-104279-choywmwd	121	24	the	the	DT
cord-104279-choywmwd	121	25	0.8-kbp	0.8-kbp	CD
cord-104279-choywmwd	121	26	MluI	MluI	NNP
cord-104279-choywmwd	121	27	-	-	HYPH
cord-104279-choywmwd	121	28	Hpal	Hpal	NNP
cord-104279-choywmwd	121	29	(	(	-LRB-
cord-104279-choywmwd	121	30	MluI	MluI	NNP
cord-104279-choywmwd	121	31	blunt	blunt	NN
cord-104279-choywmwd	121	32	ended	end	VBD
cord-104279-choywmwd	121	33	)	)	-RRB-
cord-104279-choywmwd	122	1	STE/3	STE/3	NNP
cord-104279-choywmwd	122	2	fragment	fragment	NN
cord-104279-choywmwd	122	3	into	into	IN
cord-104279-choywmwd	122	4	the	the	DT
cord-104279-choywmwd	122	5	Sinai	Sinai	NNP
cord-104279-choywmwd	122	6	site	site	NN
cord-104279-choywmwd	122	7	of	of	IN
cord-104279-choywmwd	122	8	pEXP1	pexp1	NN
cord-104279-choywmwd	122	9	,	,	,
cord-104279-choywmwd	122	10	an	an	DT
cord-104279-choywmwd	122	11	E.	E.	NNP
cord-104279-choywmwd	122	12	coli	coli	VBZ
cord-104279-choywmwd	122	13	expression	expression	NN
cord-104279-choywmwd	122	14	vector	vector	NN
cord-104279-choywmwd	122	15	containing	contain	VBG
cord-104279-choywmwd	122	16	the	the	DT
cord-104279-choywmwd	122	17	Tac	Tac	NNP
cord-104279-choywmwd	122	18	promoter	promoter	NN
cord-104279-choywmwd	122	19	just	just	RB
cord-104279-choywmwd	122	20	upstream	upstream	RB
cord-104279-choywmwd	122	21	of	of	IN
cord-104279-choywmwd	122	22	the	the	DT
cord-104279-choywmwd	122	23	translational	translational	JJ
cord-104279-choywmwd	122	24	start	start	NN
cord-104279-choywmwd	122	25	codon	codon	NN
cord-104279-choywmwd	122	26	of	of	IN
cord-104279-choywmwd	122	27	T4	T4	NNP
cord-104279-choywmwd	122	28	lysozyme	lysozyme	NNS
cord-104279-choywmwd	122	29	and	and	CC
cord-104279-choywmwd	122	30	a	a	DT
cord-104279-choywmwd	122	31	multiple	multiple	JJ
cord-104279-choywmwd	122	32	cloning	cloning	NN
cord-104279-choywmwd	122	33	site	site	NN
cord-104279-choywmwd	122	34	(	(	-LRB-
cord-104279-choywmwd	122	35	Raymond	Raymond	NNP
cord-104279-choywmwd	122	36	et	et	NNP
cord-104279-choywmwd	122	37	al	al	NNP
cord-104279-choywmwd	122	38	.	.	NNP
cord-104279-choywmwd	122	39	,	,	,
cord-104279-choywmwd	122	40	1990	1990	CD
cord-104279-choywmwd	122	41	)	)	-RRB-
cord-104279-choywmwd	122	42	.	.	.
cord-104279-choywmwd	123	1	A	a	DT
cord-104279-choywmwd	123	2	27	27	CD
cord-104279-choywmwd	123	3	amino	amino	NN
cord-104279-choywmwd	123	4	acid	acid	NN
cord-104279-choywmwd	123	5	peptide	peptide	NN
cord-104279-choywmwd	123	6	,	,	,
cord-104279-choywmwd	123	7	corresponding	correspond	VBG
cord-104279-choywmwd	123	8	to	to	IN
cord-104279-choywmwd	123	9	the	the	DT
cord-104279-choywmwd	123	10	26	26	CD
cord-104279-choywmwd	123	11	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	123	12	residues	residue	NNS
cord-104279-choywmwd	123	13	of	of	IN
cord-104279-choywmwd	123	14	DPAP	DPAP	NNP
cord-104279-choywmwd	123	15	B	B	NNP
cord-104279-choywmwd	123	16	followed	follow	VBN
cord-104279-choywmwd	123	17	by	by	IN
cord-104279-choywmwd	123	18	a	a	DT
cord-104279-choywmwd	123	19	COOH	COOH	NNP
cord-104279-choywmwd	123	20	-	-	HYPH
cord-104279-choywmwd	123	21	terminal	terminal	NN
cord-104279-choywmwd	123	22	cysteine	cysteine	NN
cord-104279-choywmwd	123	23	residue	residue	NN
cord-104279-choywmwd	123	24	,	,	,
cord-104279-choywmwd	123	25	was	be	VBD
cord-104279-choywmwd	123	26	synthesized	synthesize	VBN
cord-104279-choywmwd	123	27	on	on	IN
cord-104279-choywmwd	123	28	an	an	DT
cord-104279-choywmwd	123	29	Applied	Applied	NNP
cord-104279-choywmwd	123	30	Biosystems	Biosystems	NNP
cord-104279-choywmwd	123	31	Peptide	Peptide	NNP
cord-104279-choywmwd	123	32	Synthesizer	Synthesizer	NNP
cord-104279-choywmwd	123	33	.	.	.
cord-104279-choywmwd	124	1	The	the	DT
cord-104279-choywmwd	124	2	peptide	peptide	NN
cord-104279-choywmwd	124	3	was	be	VBD
cord-104279-choywmwd	124	4	coupled	couple	VBN
cord-104279-choywmwd	124	5	to	to	TO
cord-104279-choywmwd	124	6	carboxymethylated	carboxymethylate	VBN
cord-104279-choywmwd	124	7	BSA	BSA	NNP
cord-104279-choywmwd	124	8	through	through	IN
cord-104279-choywmwd	124	9	the	the	DT
cord-104279-choywmwd	124	10	COOH	COOH	NNP
cord-104279-choywmwd	124	11	-	-	HYPH
cord-104279-choywmwd	124	12	terminal	terminal	NN
cord-104279-choywmwd	124	13	cysteine	cysteine	NN
cord-104279-choywmwd	124	14	with	with	IN
cord-104279-choywmwd	124	15	the	the	DT
cord-104279-choywmwd	124	16	hifunctional	hifunctional	JJ
cord-104279-choywmwd	124	17	crosslinking	crosslinking	NN
cord-104279-choywmwd	124	18	agent	agent	NN
cord-104279-choywmwd	124	19	,	,	,
cord-104279-choywmwd	124	20	MBS	MBS	NNP
cord-104279-choywmwd	124	21	,	,	,
cord-104279-choywmwd	124	22	following	follow	VBG
cord-104279-choywmwd	124	23	the	the	DT
cord-104279-choywmwd	124	24	manufacturer	manufacturer	NN
cord-104279-choywmwd	124	25	's	's	POS
cord-104279-choywmwd	124	26	recommendations	recommendation	NNS
cord-104279-choywmwd	124	27	(	(	-LRB-
cord-104279-choywmwd	124	28	Pierce	Pierce	NNP
cord-104279-choywmwd	124	29	Chemical	Chemical	NNP
cord-104279-choywmwd	124	30	Co.	Co.	NNP
cord-104279-choywmwd	124	31	,	,	,
cord-104279-choywmwd	124	32	Rockford	Rockford	NNP
cord-104279-choywmwd	124	33	,	,	,
cord-104279-choywmwd	124	34	IL	IL	NNP
cord-104279-choywmwd	124	35	)	)	-RRB-
cord-104279-choywmwd	124	36	,	,	,
cord-104279-choywmwd	124	37	and	and	CC
cord-104279-choywmwd	124	38	through	through	IN
cord-104279-choywmwd	124	39	lysine	lysine	NN
cord-104279-choywmwd	124	40	residues	residue	NNS
cord-104279-choywmwd	124	41	with	with	IN
cord-104279-choywmwd	124	42	gluteraldehyde	gluteraldehyde	NNP
cord-104279-choywmwd	124	43	as	as	IN
cord-104279-choywmwd	124	44	previously	previously	RB
cord-104279-choywmwd	124	45	described	describe	VBN
cord-104279-choywmwd	124	46	(	(	-LRB-
cord-104279-choywmwd	124	47	Kagen	Kagen	NNP
cord-104279-choywmwd	124	48	and	and	CC
cord-104279-choywmwd	124	49	Glick	Glick	NNP
cord-104279-choywmwd	124	50	,	,	,
cord-104279-choywmwd	124	51	1979	1979	CD
cord-104279-choywmwd	124	52	)	)	-RRB-
cord-104279-choywmwd	124	53	.	.	.
cord-104279-choywmwd	125	1	A	a	DT
cord-104279-choywmwd	125	2	1:1	1:1	CD
cord-104279-choywmwd	125	3	mixture	mixture	NN
cord-104279-choywmwd	125	4	of	of	IN
cord-104279-choywmwd	125	5	BSA	BSA	NNP
cord-104279-choywmwd	125	6	-	-	HYPH
cord-104279-choywmwd	125	7	peptide	peptide	NN
cord-104279-choywmwd	125	8	conjugates	conjugate	NNS
cord-104279-choywmwd	125	9	prepared	prepare	VBN
cord-104279-choywmwd	125	10	by	by	IN
cord-104279-choywmwd	125	11	the	the	DT
cord-104279-choywmwd	125	12	two	two	CD
cord-104279-choywmwd	125	13	methods	method	NNS
cord-104279-choywmwd	125	14	was	be	VBD
cord-104279-choywmwd	125	15	used	use	VBN
cord-104279-choywmwd	125	16	to	to	TO
cord-104279-choywmwd	125	17	immunize	immunize	VB
cord-104279-choywmwd	125	18	rabbits	rabbit	NNS
cord-104279-choywmwd	125	19	as	as	IN
cord-104279-choywmwd	125	20	described	describe	VBN
cord-104279-choywmwd	125	21	previously	previously	RB
cord-104279-choywmwd	125	22	(	(	-LRB-
cord-104279-choywmwd	125	23	Vaitukaitis	Vaitukaitis	NNP
cord-104279-choywmwd	125	24	,	,	,
cord-104279-choywmwd	125	25	1981	1981	CD
cord-104279-choywmwd	125	26	)	)	-RRB-
cord-104279-choywmwd	125	27	.	.	.
cord-104279-choywmwd	126	1	For	for	IN
cord-104279-choywmwd	126	2	affinity	affinity	NN
cord-104279-choywmwd	126	3	purification	purification	NN
cord-104279-choywmwd	126	4	of	of	IN
cord-104279-choywmwd	126	5	the	the	DT
cord-104279-choywmwd	126	6	antibody	antibody	NN
cord-104279-choywmwd	126	7	,	,	,
cord-104279-choywmwd	126	8	the	the	DT
cord-104279-choywmwd	126	9	poptide	poptide	NN
cord-104279-choywmwd	126	10	was	be	VBD
cord-104279-choywmwd	126	11	cross	cross	JJ
cord-104279-choywmwd	126	12	-	-	VBN
cord-104279-choywmwd	126	13	linked	link	VBN
cord-104279-choywmwd	126	14	to	to	IN
cord-104279-choywmwd	126	15	Tresyl	Tresyl	NNP
cord-104279-choywmwd	126	16	-	-	HYPH
cord-104279-choywmwd	126	17	activated	activate	VBN
cord-104279-choywmwd	126	18	Sepharose	Sepharose	NNP
cord-104279-choywmwd	126	19	4B	4b	NN
cord-104279-choywmwd	126	20	(	(	-LRB-
cord-104279-choywmwd	126	21	Pharmacia	Pharmacia	NNP
cord-104279-choywmwd	126	22	Fine	Fine	NNP
cord-104279-choywmwd	126	23	Chemicals	Chemicals	NNPS
cord-104279-choywmwd	126	24	)	)	-RRB-
cord-104279-choywmwd	126	25	,	,	,
cord-104279-choywmwd	126	26	and	and	CC
cord-104279-choywmwd	126	27	affinity	affinity	NN
cord-104279-choywmwd	126	28	purification	purification	NN
cord-104279-choywmwd	126	29	was	be	VBD
cord-104279-choywmwd	126	30	carded	card	VBN
cord-104279-choywmwd	126	31	out	out	RP
cord-104279-choywmwd	126	32	as	as	IN
cord-104279-choywmwd	126	33	described	describe	VBN
cord-104279-choywmwd	126	34	(	(	-LRB-
cord-104279-choywmwd	126	35	Raymond	Raymond	NNP
cord-104279-choywmwd	126	36	et	et	NNP
cord-104279-choywmwd	126	37	al	al	NNP
cord-104279-choywmwd	126	38	.	.	NNP
cord-104279-choywmwd	126	39	,	,	,
cord-104279-choywmwd	126	40	1990	1990	CD
cord-104279-choywmwd	126	41	)	)	-RRB-
cord-104279-choywmwd	126	42	.	.	.
cord-104279-choywmwd	127	1	DPAP	DPAP	NNP
cord-104279-choywmwd	127	2	A	a	DT
cord-104279-choywmwd	127	3	antigen	antigen	NN
cord-104279-choywmwd	127	4	was	be	VBD
cord-104279-choywmwd	127	5	produced	produce	VBN
cord-104279-choywmwd	127	6	in	in	IN
cord-104279-choywmwd	127	7	E.	E.	NNP
cord-104279-choywmwd	127	8	coli	coli	NNS
cord-104279-choywmwd	127	9	cells	cell	NNS
cord-104279-choywmwd	127	10	containing	contain	VBG
cord-104279-choywmwd	127	11	the	the	DT
cord-104279-choywmwd	127	12	plasmid	plasmid	NN
cord-104279-choywmwd	127	13	pC	pc	NN
cord-104279-choywmwd	127	14	JR24	JR24	NNP
cord-104279-choywmwd	127	15	.	.	.
cord-104279-choywmwd	128	1	Induction	induction	NN
cord-104279-choywmwd	128	2	with	with	IN
cord-104279-choywmwd	128	3	IPTG	IPTG	NNP
cord-104279-choywmwd	128	4	resulted	result	VBD
cord-104279-choywmwd	128	5	in	in	IN
cord-104279-choywmwd	128	6	the	the	DT
cord-104279-choywmwd	128	7	production	production	NN
cord-104279-choywmwd	128	8	ofa	ofa	NNP
cord-104279-choywmwd	128	9	31-kD	31-kd	CD
cord-104279-choywmwd	128	10	protein	protein	NN
cord-104279-choywmwd	128	11	that	that	WDT
cord-104279-choywmwd	128	12	corresponds	correspond	VBZ
cord-104279-choywmwd	128	13	to	to	IN
cord-104279-choywmwd	128	14	the	the	DT
cord-104279-choywmwd	128	15	NHE	NHE	NNP
cord-104279-choywmwd	128	16	-	-	HYPH
cord-104279-choywmwd	128	17	terminal	terminal	JJ
cord-104279-choywmwd	128	18	portion	portion	NN
cord-104279-choywmwd	128	19	of	of	IN
cord-104279-choywmwd	128	20	the	the	DT
cord-104279-choywmwd	128	21	lumenal	lumenal	JJ
cord-104279-choywmwd	128	22	domain	domain	NN
cord-104279-choywmwd	128	23	of	of	IN
cord-104279-choywmwd	128	24	DPAP	DPAP	NNP
cord-104279-choywmwd	128	25	A	A	NNP
cord-104279-choywmwd	129	1	(	(	-LRB-
cord-104279-choywmwd	129	2	see	see	VB
cord-104279-choywmwd	129	3	Fig	Fig	NNP
cord-104279-choywmwd	129	4	.	.	.
cord-104279-choywmwd	129	5	6	6	CD
cord-104279-choywmwd	129	6	)	)	-RRB-
cord-104279-choywmwd	129	7	fused	fuse	VBD
cord-104279-choywmwd	129	8	to	to	IN
cord-104279-choywmwd	129	9	seven	seven	CD
cord-104279-choywmwd	129	10	residues	residue	NNS
cord-104279-choywmwd	129	11	of	of	IN
cord-104279-choywmwd	129	12	T4	T4	NNP
cord-104279-choywmwd	129	13	lysosyme	lysosyme	NNS
cord-104279-choywmwd	129	14	and	and	CC
cord-104279-choywmwd	129	15	seven	seven	CD
cord-104279-choywmwd	129	16	residues	residue	NNS
cord-104279-choywmwd	129	17	encoded	encode	VBN
cord-104279-choywmwd	129	18	by	by	IN
cord-104279-choywmwd	129	19	the	the	DT
cord-104279-choywmwd	129	20	pEXPI	pEXPI	NNP
cord-104279-choywmwd	129	21	polylinker	polylinker	NNP
cord-104279-choywmwd	129	22	.	.	.
cord-104279-choywmwd	130	1	Antigen	Antigen	NNP
cord-104279-choywmwd	130	2	purification	purification	NN
cord-104279-choywmwd	130	3	was	be	VBD
cord-104279-choywmwd	130	4	performed	perform	VBN
cord-104279-choywmwd	130	5	as	as	IN
cord-104279-choywmwd	130	6	described	describe	VBN
cord-104279-choywmwd	130	7	previously	previously	RB
cord-104279-choywmwd	130	8	(	(	-LRB-
cord-104279-choywmwd	130	9	Raymond	Raymond	NNP
cord-104279-choywmwd	130	10	et	et	NNP
cord-104279-choywmwd	130	11	al	al	NNP
cord-104279-choywmwd	130	12	.	.	NNP
cord-104279-choywmwd	130	13	,	,	,
cord-104279-choywmwd	130	14	1990	1990	CD
cord-104279-choywmwd	130	15	)	)	-RRB-
cord-104279-choywmwd	130	16	.	.	.
cord-104279-choywmwd	131	1	Immunoprecipitations	immunoprecipitation	NNS
cord-104279-choywmwd	131	2	were	be	VBD
cord-104279-choywmwd	131	3	performed	perform	VBN
cord-104279-choywmwd	131	4	by	by	IN
cord-104279-choywmwd	131	5	growing	grow	VBG
cord-104279-choywmwd	131	6	cultures	culture	NNS
cord-104279-choywmwd	131	7	to	to	TO
cord-104279-choywmwd	131	8	log	log	VB
cord-104279-choywmwd	131	9	phase	phase	NN
cord-104279-choywmwd	131	10	in	in	IN
cord-104279-choywmwd	131	11	supplemented	supplement	VBN
cord-104279-choywmwd	131	12	minimal	minimal	JJ
cord-104279-choywmwd	131	13	media	medium	NNS
cord-104279-choywmwd	131	14	lacking	lack	VBG
cord-104279-choywmwd	131	15	methionine	methionine	NN
cord-104279-choywmwd	131	16	and	and	CC
cord-104279-choywmwd	131	17	cysteine	cysteine	NN
cord-104279-choywmwd	131	18	,	,	,
cord-104279-choywmwd	131	19	then	then	RB
cord-104279-choywmwd	131	20	pulse	pulse	NN
cord-104279-choywmwd	131	21	labeling	labeling	NN
cord-104279-choywmwd	131	22	in	in	IN
cord-104279-choywmwd	131	23	the	the	DT
cord-104279-choywmwd	131	24	same	same	JJ
cord-104279-choywmwd	131	25	media	medium	NNS
cord-104279-choywmwd	131	26	with	with	IN
cord-104279-choywmwd	131	27	Tran3SS	tran3ss	NN
cord-104279-choywmwd	131	28	-	-	HYPH
cord-104279-choywmwd	131	29	label	label	NN
cord-104279-choywmwd	131	30	and	and	CC
cord-104279-choywmwd	131	31	chasing	chase	VBG
cord-104279-choywmwd	131	32	by	by	IN
cord-104279-choywmwd	131	33	adding	add	VBG
cord-104279-choywmwd	131	34	50	50	CD
cord-104279-choywmwd	132	1	/~g	/~g	NFP
cord-104279-choywmwd	132	2	/	/	SYM
cord-104279-choywmwd	132	3	mL	ml	NN
cord-104279-choywmwd	132	4	methionine	methionine	NN
cord-104279-choywmwd	132	5	and	and	CC
cord-104279-choywmwd	132	6	50/~g	50/~g	NNP
cord-104279-choywmwd	132	7	/	/	SYM
cord-104279-choywmwd	132	8	mL	ml	NN
cord-104279-choywmwd	132	9	cysteine	cysteine	NN
cord-104279-choywmwd	132	10	,	,	,
cord-104279-choywmwd	132	11	followed	follow	VBN
cord-104279-choywmwd	132	12	by	by	IN
cord-104279-choywmwd	132	13	the	the	DT
cord-104279-choywmwd	132	14	addition	addition	NN
cord-104279-choywmwd	132	15	of	of	IN
cord-104279-choywmwd	132	16	sodium	sodium	NN
cord-104279-choywmwd	132	17	azide	azide	NN
cord-104279-choywmwd	132	18	to	to	IN
cord-104279-choywmwd	132	19	10	10	CD
cord-104279-choywmwd	132	20	raM.	ram.	NN
cord-104279-choywmwd	133	1	The	the	DT
cord-104279-choywmwd	133	2	cells	cell	NNS
cord-104279-choywmwd	133	3	were	be	VBD
cord-104279-choywmwd	133	4	converted	convert	VBN
cord-104279-choywmwd	133	5	to	to	IN
cord-104279-choywmwd	133	6	spheroplasts	spheroplasts	NNP
cord-104279-choywmwd	133	7	(	(	-LRB-
cord-104279-choywmwd	133	8	Stevens	Stevens	NNP
cord-104279-choywmwd	133	9	et	et	NNP
cord-104279-choywmwd	133	10	al	al	NNP
cord-104279-choywmwd	133	11	.	.	NNP
cord-104279-choywmwd	133	12	,	,	,
cord-104279-choywmwd	133	13	1986	1986	CD
cord-104279-choywmwd	133	14	)	)	-RRB-
cord-104279-choywmwd	133	15	which	which	WDT
cord-104279-choywmwd	133	16	were	be	VBD
cord-104279-choywmwd	133	17	lysed	lyse	VBN
cord-104279-choywmwd	133	18	in	in	IN
cord-104279-choywmwd	133	19	1	1	CD
cord-104279-choywmwd	133	20	%	%	NN
cord-104279-choywmwd	133	21	SDS	SDS	NNP
cord-104279-choywmwd	133	22	,	,	,
cord-104279-choywmwd	133	23	8	8	CD
cord-104279-choywmwd	133	24	M	M	NNP
cord-104279-choywmwd	133	25	urea	urea	NN
cord-104279-choywmwd	133	26	plus	plus	CC
cord-104279-choywmwd	133	27	a	a	DT
cord-104279-choywmwd	133	28	protease	protease	NN
cord-104279-choywmwd	133	29	inhibitor	inhibitor	NN
cord-104279-choywmwd	133	30	cocktail	cocktail	NN
cord-104279-choywmwd	133	31	(	(	-LRB-
cord-104279-choywmwd	133	32	0.5	0.5	CD
cord-104279-choywmwd	133	33	mM	mm	CD
cord-104279-choywmwd	133	34	PMSF	PMSF	NNP
cord-104279-choywmwd	133	35	,	,	,
cord-104279-choywmwd	133	36	1/~g	1/~g	CD
cord-104279-choywmwd	133	37	/	/	SYM
cord-104279-choywmwd	133	38	mL	ml	NN
cord-104279-choywmwd	133	39	leupeptin	leupeptin	NN
cord-104279-choywmwd	133	40	,	,	,
cord-104279-choywmwd	133	41	and	and	CC
cord-104279-choywmwd	133	42	1	1	CD
cord-104279-choywmwd	133	43	/~g	/~g	CD
cord-104279-choywmwd	133	44	/	/	SYM
cord-104279-choywmwd	133	45	mL	ml	NN
cord-104279-choywmwd	133	46	pepstatin	pepstatin	NNP
cord-104279-choywmwd	133	47	)	)	-RRB-
cord-104279-choywmwd	133	48	for	for	IN
cord-104279-choywmwd	133	49	5	5	CD
cord-104279-choywmwd	133	50	rain	rain	NN
cord-104279-choywmwd	133	51	at	at	IN
cord-104279-choywmwd	133	52	100~	100~	CD
cord-104279-choywmwd	133	53	and	and	CC
cord-104279-choywmwd	133	54	adjusted	adjust	VBN
cord-104279-choywmwd	133	55	to	to	IN
cord-104279-choywmwd	133	56	1	1	CD
cord-104279-choywmwd	133	57	mL	ml	NN
cord-104279-choywmwd	133	58	in	in	IN
cord-104279-choywmwd	133	59	IP	ip	NN
cord-104279-choywmwd	133	60	buffer	buffer	NN
cord-104279-choywmwd	133	61	(	(	-LRB-
cord-104279-choywmwd	134	1	PBS	PBS	NNP
cord-104279-choywmwd	134	2	,	,	,
cord-104279-choywmwd	134	3	0.1	0.1	CD
cord-104279-choywmwd	134	4	%	%	NN
cord-104279-choywmwd	134	5	SDS	sds	NN
cord-104279-choywmwd	134	6	,	,	,
cord-104279-choywmwd	134	7	0.1	0.1	CD
cord-104279-choywmwd	134	8	%	%	NN
cord-104279-choywmwd	134	9	Triton	Triton	NNP
cord-104279-choywmwd	134	10	X-100	x-100	NN
cord-104279-choywmwd	134	11	,	,	,
cord-104279-choywmwd	134	12	1	1	CD
cord-104279-choywmwd	134	13	mivl	mivl	NN
cord-104279-choywmwd	134	14	EDTA	EDTA	NNP
cord-104279-choywmwd	134	15	)	)	-RRB-
cord-104279-choywmwd	134	16	.	.	.
cord-104279-choywmwd	135	1	For	for	IN
cord-104279-choywmwd	135	2	precipitating	precipitate	VBG
cord-104279-choywmwd	135	3	~Fss	~fss	NN
cord-104279-choywmwd	135	4	-	-	HYPH
cord-104279-choywmwd	135	5	B	b	NN
cord-104279-choywmwd	135	6	from	from	IN
cord-104279-choywmwd	135	7	the	the	DT
cord-104279-choywmwd	135	8	extracellular	extracellular	JJ
cord-104279-choywmwd	135	9	fractions	fraction	NNS
cord-104279-choywmwd	135	10	,	,	,
cord-104279-choywmwd	135	11	the	the	DT
cord-104279-choywmwd	135	12	medium	medium	NN
cord-104279-choywmwd	135	13	was	be	VBD
cord-104279-choywmwd	135	14	supplemented	supplement	VBN
cord-104279-choywmwd	135	15	with	with	IN
cord-104279-choywmwd	135	16	2	2	CD
cord-104279-choywmwd	135	17	m4,,/mL	m4,,/mL	NNP
cord-104279-choywmwd	135	18	BSA	BSA	NNP
cord-104279-choywmwd	135	19	and	and	CC
cord-104279-choywmwd	135	20	50	50	CD
cord-104279-choywmwd	135	21	mM	mm	CD
cord-104279-choywmwd	135	22	potassium	potassium	NN
cord-104279-choywmwd	135	23	phosphate	phosphate	NN
cord-104279-choywmwd	135	24	,	,	,
cord-104279-choywmwd	135	25	pH	pH	NNP
cord-104279-choywmwd	135	26	5.7	5.7	CD
cord-104279-choywmwd	135	27	.	.	.
cord-104279-choywmwd	136	1	After	after	IN
cord-104279-choywmwd	136	2	spberoplasting	spberoplaste	VBG
cord-104279-choywmwd	136	3	,	,	,
cord-104279-choywmwd	136	4	the	the	DT
cord-104279-choywmwd	136	5	periplasmic	periplasmic	JJ
cord-104279-choywmwd	136	6	and	and	CC
cord-104279-choywmwd	136	7	media	medium	NNS
cord-104279-choywmwd	136	8	fractions	fraction	NNS
cord-104279-choywmwd	136	9	were	be	VBD
cord-104279-choywmwd	136	10	pooled	pool	VBN
cord-104279-choywmwd	136	11	and	and	CC
cord-104279-choywmwd	136	12	adjusted	adjust	VBN
cord-104279-choywmwd	136	13	to	to	IN
cord-104279-choywmwd	136	14	1	1	CD
cord-104279-choywmwd	136	15	mL	ml	NN
cord-104279-choywmwd	136	16	in	in	IN
cord-104279-choywmwd	136	17	IP	ip	NN
cord-104279-choywmwd	136	18	buffer	buffer	NN
cord-104279-choywmwd	136	19	plus	plus	IN
cord-104279-choywmwd	136	20	protease	protease	NN
cord-104279-choywmwd	136	21	inhibitors	inhibitor	NNS
cord-104279-choywmwd	136	22	.	.	.
cord-104279-choywmwd	137	1	After	after	IN
cord-104279-choywmwd	137	2	pre	pre	JJ
cord-104279-choywmwd	137	3	-	-	NN
cord-104279-choywmwd	137	4	adsorbtion	adsorbtion	NN
cord-104279-choywmwd	137	5	to	to	IN
cord-104279-choywmwd	137	6	0.5	0.5	CD
cord-104279-choywmwd	137	7	%	%	NN
cord-104279-choywmwd	137	8	IgGSorb	IgGSorb	NNP
cord-104279-choywmwd	137	9	,	,	,
cord-104279-choywmwd	137	10	anti	anti	JJ
cord-104279-choywmwd	137	11	-	-	JJ
cord-104279-choywmwd	137	12	DPAP	dpap	JJ
cord-104279-choywmwd	137	13	B	B	NNP
cord-104279-choywmwd	137	14	COOHterminal	coohterminal	JJ
cord-104279-choywmwd	137	15	antibody	antibody	NN
cord-104279-choywmwd	137	16	(	(	-LRB-
cord-104279-choywmwd	137	17	Roberts	Roberts	NNP
cord-104279-choywmwd	137	18	et	et	NNP
cord-104279-choywmwd	137	19	al	al	NNP
cord-104279-choywmwd	137	20	.	.	NNP
cord-104279-choywmwd	137	21	,	,	,
cord-104279-choywmwd	137	22	1989	1989	CD
cord-104279-choywmwd	137	23	)	)	-RRB-
cord-104279-choywmwd	137	24	,	,	,
cord-104279-choywmwd	137	25	or	or	CC
cord-104279-choywmwd	137	26	anti	anti	JJ
cord-104279-choywmwd	137	27	-	-	JJ
cord-104279-choywmwd	137	28	DPAP	DPAP	NNP
cord-104279-choywmwd	137	29	A	a	DT
cord-104279-choywmwd	137	30	antibody	antibody	NN
cord-104279-choywmwd	137	31	was	be	VBD
cord-104279-choywmwd	137	32	added	add	VBN
cord-104279-choywmwd	137	33	,	,	,
cord-104279-choywmwd	137	34	and	and	CC
cord-104279-choywmwd	137	35	samples	sample	NNS
cord-104279-choywmwd	137	36	were	be	VBD
cord-104279-choywmwd	137	37	incubated	incubate	VBN
cord-104279-choywmwd	137	38	one	one	CD
cord-104279-choywmwd	137	39	hour	hour	NN
cord-104279-choywmwd	137	40	on	on	IN
cord-104279-choywmwd	137	41	ice	ice	NN
cord-104279-choywmwd	137	42	.	.	.
cord-104279-choywmwd	138	1	IgGSorb	IgGSorb	NNP
cord-104279-choywmwd	138	2	was	be	VBD
cord-104279-choywmwd	138	3	added	add	VBN
cord-104279-choywmwd	138	4	to	to	IN
cord-104279-choywmwd	138	5	0.5	0.5	CD
cord-104279-choywmwd	138	6	%	%	NN
cord-104279-choywmwd	138	7	,	,	,
cord-104279-choywmwd	138	8	followed	follow	VBN
cord-104279-choywmwd	138	9	by	by	IN
cord-104279-choywmwd	138	10	1	1	CD
cord-104279-choywmwd	138	11	h	h	NN
cord-104279-choywmwd	138	12	on	on	IN
cord-104279-choywmwd	138	13	ice	ice	NN
cord-104279-choywmwd	138	14	,	,	,
cord-104279-choywmwd	138	15	and	and	CC
cord-104279-choywmwd	138	16	the	the	DT
cord-104279-choywmwd	138	17	immune	immune	JJ
cord-104279-choywmwd	138	18	complexes	complex	NNS
cord-104279-choywmwd	138	19	were	be	VBD
cord-104279-choywmwd	138	20	precipitated	precipitate	VBN
cord-104279-choywmwd	138	21	and	and	CC
cord-104279-choywmwd	138	22	washed	wash	VBN
cord-104279-choywmwd	138	23	twice	twice	RB
cord-104279-choywmwd	138	24	with	with	IN
cord-104279-choywmwd	138	25	IP	ip	NN
cord-104279-choywmwd	138	26	buffer	buffer	NN
cord-104279-choywmwd	138	27	.	.	.
cord-104279-choywmwd	139	1	The	the	DT
cord-104279-choywmwd	139	2	immune	immune	JJ
cord-104279-choywmwd	139	3	complexes	complex	NNS
cord-104279-choywmwd	139	4	were	be	VBD
cord-104279-choywmwd	139	5	solubilized	solubilized	JJ
cord-104279-choywmwd	139	6	,	,	,
cord-104279-choywmwd	139	7	and	and	CC
cord-104279-choywmwd	139	8	half	half	NN
cord-104279-choywmwd	139	9	of	of	IN
cord-104279-choywmwd	139	10	the	the	DT
cord-104279-choywmwd	139	11	samples	sample	NNS
cord-104279-choywmwd	139	12	were	be	VBD
cord-104279-choywmwd	139	13	treated	treat	VBN
cord-104279-choywmwd	139	14	with	with	IN
cord-104279-choywmwd	139	15	endoglycosidase	endoglycosidase	NNP
cord-104279-choywmwd	139	16	H	H	NNP
cord-104279-choywmwd	139	17	(	(	-LRB-
cord-104279-choywmwd	139	18	Endo	Endo	NNP
cord-104279-choywmwd	139	19	H	H	NNP
cord-104279-choywmwd	139	20	)	)	-RRB-
cord-104279-choywmwd	139	21	overnight	overnight	RB
cord-104279-choywmwd	139	22	at	at	IN
cord-104279-choywmwd	139	23	37~	37~	CD
cord-104279-choywmwd	139	24	as	as	IN
cord-104279-choywmwd	139	25	described	describe	VBN
cord-104279-choywmwd	139	26	(	(	-LRB-
cord-104279-choywmwd	139	27	Orlean	Orlean	NNP
cord-104279-choywmwd	139	28	et	et	NNP
cord-104279-choywmwd	139	29	al	al	NNP
cord-104279-choywmwd	139	30	.	.	NNP
cord-104279-choywmwd	139	31	,	,	,
cord-104279-choywmwd	139	32	1991	1991	CD
cord-104279-choywmwd	139	33	)	)	-RRB-
cord-104279-choywmwd	139	34	.	.	.
cord-104279-choywmwd	140	1	Samples	sample	NNS
cord-104279-choywmwd	140	2	were	be	VBD
cord-104279-choywmwd	140	3	analyzed	analyze	VBN
cord-104279-choywmwd	140	4	by	by	IN
cord-104279-choywmwd	140	5	SDS	SDS	NNP
cord-104279-choywmwd	140	6	-	-	HYPH
cord-104279-choywmwd	140	7	PAGE	PAGE	NNP
cord-104279-choywmwd	140	8	and	and	CC
cord-104279-choywmwd	140	9	fluorography	fluorography	NN
cord-104279-choywmwd	140	10	as	as	IN
cord-104279-choywmwd	140	11	described	describe	VBN
cord-104279-choywmwd	140	12	previously	previously	RB
cord-104279-choywmwd	141	1	(	(	-LRB-
cord-104279-choywmwd	141	2	Stevens	Stevens	NNP
cord-104279-choywmwd	141	3	et	et	NNP
cord-104279-choywmwd	141	4	ai	ai	NNP
cord-104279-choywmwd	141	5	.	.	NNP
cord-104279-choywmwd	141	6	,	,	,
cord-104279-choywmwd	141	7	1986	1986	CD
cord-104279-choywmwd	141	8	)	)	-RRB-
cord-104279-choywmwd	141	9	.	.	.
cord-104279-choywmwd	142	1	The	the	DT
cord-104279-choywmwd	142	2	secretion	secretion	NN
cord-104279-choywmwd	142	3	of	of	IN
cord-104279-choywmwd	142	4	t	t	NN
cord-104279-choywmwd	142	5	~	~	CD
cord-104279-choywmwd	142	6	Fss	Fss	NNP
cord-104279-choywmwd	142	7	-	-	HYPH
cord-104279-choywmwd	142	8	B	B	NNP
cord-104279-choywmwd	142	9	was	be	VBD
cord-104279-choywmwd	142	10	quantified	quantify	VBN
cord-104279-choywmwd	142	11	using	use	VBG
cord-104279-choywmwd	142	12	an	an	DT
cord-104279-choywmwd	142	13	Ambis	Ambis	NNP
cord-104279-choywmwd	142	14	Radioanalytic	Radioanalytic	NNP
cord-104279-choywmwd	142	15	Imaging	Imaging	NNP
cord-104279-choywmwd	142	16	System	System	NNP
cord-104279-choywmwd	142	17	(	(	-LRB-
cord-104279-choywmwd	142	18	Ambis	Ambis	NNP
cord-104279-choywmwd	142	19	Systems	Systems	NNP
cord-104279-choywmwd	142	20	,	,	,
cord-104279-choywmwd	142	21	Inc.	Inc.	NNP
cord-104279-choywmwd	142	22	,	,	,
cord-104279-choywmwd	142	23	San	San	NNP
cord-104279-choywmwd	142	24	Diego	Diego	NNP
cord-104279-choywmwd	142	25	,	,	,
cord-104279-choywmwd	142	26	CA	CA	NNP
cord-104279-choywmwd	142	27	)	)	-RRB-
cord-104279-choywmwd	142	28	.	.	.
cord-104279-choywmwd	143	1	The	the	DT
cord-104279-choywmwd	143	2	fractionation	fractionation	NN
cord-104279-choywmwd	143	3	of	of	IN
cord-104279-choywmwd	143	4	membranes	membrane	NNS
cord-104279-choywmwd	143	5	in	in	IN
cord-104279-choywmwd	143	6	the	the	DT
cord-104279-choywmwd	143	7	presence	presence	NN
cord-104279-choywmwd	143	8	of	of	IN
cord-104279-choywmwd	143	9	high	high	JJ
cord-104279-choywmwd	143	10	pH	pH	NNP
cord-104279-choywmwd	143	11	sodium	sodium	NN
cord-104279-choywmwd	143	12	carbonate	carbonate	NN
cord-104279-choywmwd	143	13	was	be	VBD
cord-104279-choywmwd	143	14	performed	perform	VBN
cord-104279-choywmwd	143	15	as	as	IN
cord-104279-choywmwd	143	16	described	describe	VBN
cord-104279-choywmwd	143	17	(	(	-LRB-
cord-104279-choywmwd	143	18	Roberts	Roberts	NNP
cord-104279-choywmwd	143	19	et	et	NNP
cord-104279-choywmwd	143	20	al	al	NNP
cord-104279-choywmwd	143	21	.	.	NNP
cord-104279-choywmwd	143	22	,	,	,
cord-104279-choywmwd	143	23	1989	1989	CD
cord-104279-choywmwd	143	24	)	)	-RRB-
cord-104279-choywmwd	143	25	.	.	.
cord-104279-choywmwd	144	1	Preparation	preparation	NN
cord-104279-choywmwd	144	2	of	of	IN
cord-104279-choywmwd	144	3	fixed	fix	VBN
cord-104279-choywmwd	144	4	,	,	,
cord-104279-choywmwd	144	5	spheroplasted	spheroplasted	JJ
cord-104279-choywmwd	144	6	cells	cell	NNS
cord-104279-choywmwd	144	7	for	for	IN
cord-104279-choywmwd	144	8	indirect	indirect	JJ
cord-104279-choywmwd	144	9	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	144	10	was	be	VBD
cord-104279-choywmwd	144	11	carried	carry	VBN
cord-104279-choywmwd	144	12	out	out	RP
cord-104279-choywmwd	144	13	essentially	essentially	RB
cord-104279-choywmwd	144	14	as	as	RB
cord-104279-choywmwd	144	15	previously	previously	RB
cord-104279-choywmwd	144	16	described	describe	VBN
cord-104279-choywmwd	144	17	(	(	-LRB-
cord-104279-choywmwd	144	18	Roberts	Roberts	NNP
cord-104279-choywmwd	144	19	et	et	NNP
cord-104279-choywmwd	144	20	al	al	NNP
cord-104279-choywmwd	144	21	.	.	NNP
cord-104279-choywmwd	144	22	,	,	,
cord-104279-choywmwd	144	23	1991	1991	CD
cord-104279-choywmwd	144	24	)	)	-RRB-
cord-104279-choywmwd	144	25	,	,	,
cord-104279-choywmwd	144	26	except	except	IN
cord-104279-choywmwd	144	27	that	that	IN
cord-104279-choywmwd	144	28	the	the	DT
cord-104279-choywmwd	144	29	fixed	fix	VBN
cord-104279-choywmwd	144	30	spheroplasts	spheroplast	NNS
cord-104279-choywmwd	144	31	were	be	VBD
cord-104279-choywmwd	144	32	treated	treat	VBN
cord-104279-choywmwd	144	33	with	with	IN
cord-104279-choywmwd	144	34	1	1	CD
cord-104279-choywmwd	144	35	%	%	NN
cord-104279-choywmwd	144	36	SDS	sds	NN
cord-104279-choywmwd	144	37	for	for	IN
cord-104279-choywmwd	144	38	1	1	CD
cord-104279-choywmwd	144	39	-	-	SYM
cord-104279-choywmwd	144	40	5	5	CD
cord-104279-choywmwd	144	41	minutes	minute	NNS
cord-104279-choywmwd	144	42	.	.	.
cord-104279-choywmwd	145	1	Antibody	antibody	NN
cord-104279-choywmwd	145	2	adsorption	adsorption	NN
cord-104279-choywmwd	145	3	against	against	IN
cord-104279-choywmwd	145	4	fixed	fix	VBN
cord-104279-choywmwd	145	5	spheroplasts	spheroplast	NNS
cord-104279-choywmwd	145	6	harboring	harbor	VBG
cord-104279-choywmwd	145	7	null	null	JJ
cord-104279-choywmwd	145	8	mutations	mutation	NNS
cord-104279-choywmwd	145	9	in	in	IN
cord-104279-choywmwd	145	10	either	either	CC
cord-104279-choywmwd	145	11	DAP2	DAP2	NNP
cord-104279-choywmwd	145	12	or	or	CC
cord-104279-choywmwd	145	13	STEJ3	STEJ3	NNP
cord-104279-choywmwd	145	14	was	be	VBD
cord-104279-choywmwd	145	15	performed	perform	VBN
cord-104279-choywmwd	145	16	as	as	IN
cord-104279-choywmwd	145	17	described	describe	VBN
cord-104279-choywmwd	145	18	elsewhere	elsewhere	RB
cord-104279-choywmwd	146	1	(	(	-LRB-
cord-104279-choywmwd	146	2	Raymond	Raymond	NNP
cord-104279-choywmwd	146	3	et	et	NNP
cord-104279-choywmwd	146	4	al	al	NNP
cord-104279-choywmwd	146	5	.	.	NNP
cord-104279-choywmwd	146	6	,	,	,
cord-104279-choywmwd	146	7	1990	1990	CD
cord-104279-choywmwd	146	8	;	;	:
cord-104279-choywmwd	146	9	Roberts	Roberts	NNP
cord-104279-choywmwd	146	10	et	et	NNP
cord-104279-choywmwd	146	11	ai	ai	NNP
cord-104279-choywmwd	146	12	.	.	NNP
cord-104279-choywmwd	146	13	,	,	,
cord-104279-choywmwd	146	14	1991	1991	CD
cord-104279-choywmwd	146	15	)	)	-RRB-
cord-104279-choywmwd	146	16	.	.	.
cord-104279-choywmwd	147	1	The	the	DT
cord-104279-choywmwd	147	2	fixed	fixed	JJ
cord-104279-choywmwd	147	3	spheroplasts	spheroplast	NNS
cord-104279-choywmwd	147	4	were	be	VBD
cord-104279-choywmwd	147	5	stained	stain	VBN
cord-104279-choywmwd	147	6	with	with	IN
cord-104279-choywmwd	147	7	a	a	DT
cord-104279-choywmwd	147	8	1:10	1:10	CD
cord-104279-choywmwd	147	9	dilution	dilution	NN
cord-104279-choywmwd	147	10	of	of	IN
cord-104279-choywmwd	147	11	adsorbed	adsorb	VBN
cord-104279-choywmwd	147	12	anti	anti	JJ
cord-104279-choywmwd	147	13	-	-	JJ
cord-104279-choywmwd	147	14	DPAP	DPAP	NNP
cord-104279-choywmwd	147	15	A	A	NNP
cord-104279-choywmwd	147	16	or	or	CC
cord-104279-choywmwd	147	17	anti	anti	JJ
cord-104279-choywmwd	147	18	-	-	JJ
cord-104279-choywmwd	147	19	DPAP	dpap	JJ
cord-104279-choywmwd	147	20	B	B	NNP
cord-104279-choywmwd	147	21	affinity	affinity	NN
cord-104279-choywmwd	147	22	-	-	HYPH
cord-104279-choywmwd	147	23	purified	purify	VBN
cord-104279-choywmwd	147	24	antibody	antibody	NN
cord-104279-choywmwd	147	25	in	in	IN
cord-104279-choywmwd	147	26	PBS	PBS	NNP
cord-104279-choywmwd	147	27	-	-	HYPH
cord-104279-choywmwd	147	28	BSA	BSA	NNP
cord-104279-choywmwd	148	1	(	(	-LRB-
cord-104279-choywmwd	148	2	Roberts	Roberts	NNP
cord-104279-choywmwd	148	3	et	et	NNP
cord-104279-choywmwd	148	4	al	al	NNP
cord-104279-choywmwd	148	5	.	.	NNP
cord-104279-choywmwd	148	6	,	,	,
cord-104279-choywmwd	148	7	1991	1991	CD
cord-104279-choywmwd	148	8	)	)	-RRB-
cord-104279-choywmwd	148	9	.	.	.
cord-104279-choywmwd	149	1	For	for	IN
cord-104279-choywmwd	149	2	co	co	NN
cord-104279-choywmwd	149	3	-	-	NN
cord-104279-choywmwd	149	4	localization	localization	NN
cord-104279-choywmwd	149	5	with	with	IN
cord-104279-choywmwd	149	6	the	the	DT
cord-104279-choywmwd	149	7	60-kD	60-kd	CD
cord-104279-choywmwd	149	8	subunit	subunit	NN
cord-104279-choywmwd	149	9	of	of	IN
cord-104279-choywmwd	149	10	the	the	DT
cord-104279-choywmwd	149	11	vacuolar	vacuolar	NNP
cord-104279-choywmwd	149	12	H+-ATPase	H+-ATPase	NNP
cord-104279-choywmwd	150	1	(	(	-LRB-
cord-104279-choywmwd	150	2	Vat2p	Vat2p	VBG
cord-104279-choywmwd	150	3	,	,	,
cord-104279-choywmwd	150	4	the	the	DT
cord-104279-choywmwd	150	5	product	product	NN
cord-104279-choywmwd	150	6	of	of	IN
cord-104279-choywmwd	150	7	the	the	DT
cord-104279-choywmwd	150	8	VAT2	VAT2	NNP
cord-104279-choywmwd	150	9	gene	gene	NN
cord-104279-choywmwd	150	10	;	;	:
cord-104279-choywmwd	151	1	Yamashiro	Yamashiro	NNP
cord-104279-choywmwd	151	2	et	et	FW
cord-104279-choywmwd	151	3	al	al	NNP
cord-104279-choywmwd	151	4	.	.	NNP
cord-104279-choywmwd	151	5	,	,	,
cord-104279-choywmwd	151	6	1990	1990	CD
cord-104279-choywmwd	151	7	)	)	-RRB-
cord-104279-choywmwd	151	8	,	,	,
cord-104279-choywmwd	151	9	the	the	DT
cord-104279-choywmwd	151	10	antibody	antibody	NN
cord-104279-choywmwd	151	11	solution	solution	NN
cord-104279-choywmwd	151	12	also	also	RB
cord-104279-choywmwd	151	13	contained	contain	VBD
cord-104279-choywmwd	151	14	a	a	DT
cord-104279-choywmwd	151	15	1:10	1:10	CD
cord-104279-choywmwd	151	16	dilution	dilution	NN
cord-104279-choywmwd	151	17	of	of	IN
cord-104279-choywmwd	151	18	the	the	DT
cord-104279-choywmwd	151	19	mAb	mAb	NNS
cord-104279-choywmwd	151	20	,	,	,
cord-104279-choywmwd	151	21	13Dll	13dll	CD
cord-104279-choywmwd	151	22	(	(	-LRB-
cord-104279-choywmwd	151	23	Kane	Kane	NNP
cord-104279-choywmwd	151	24	et	et	NNP
cord-104279-choywmwd	151	25	al	al	NNP
cord-104279-choywmwd	151	26	.	.	NNP
cord-104279-choywmwd	151	27	,	,	,
cord-104279-choywmwd	151	28	1992	1992	CD
cord-104279-choywmwd	151	29	)	)	-RRB-
cord-104279-choywmwd	151	30	.	.	.
cord-104279-choywmwd	152	1	The	the	DT
cord-104279-choywmwd	152	2	DPAP	DPAP	NNP
cord-104279-choywmwd	152	3	B	B	NNP
cord-104279-choywmwd	152	4	or	or	CC
cord-104279-choywmwd	152	5	DPAP	DPAP	NNP
cord-104279-choywmwd	152	6	A	a	DT
cord-104279-choywmwd	152	7	staining	staining	NN
cord-104279-choywmwd	152	8	pattern	pattern	NN
cord-104279-choywmwd	152	9	was	be	VBD
cord-104279-choywmwd	152	10	amplified	amplify	VBN
cord-104279-choywmwd	152	11	by	by	IN
cord-104279-choywmwd	152	12	subsequent	subsequent	JJ
cord-104279-choywmwd	152	13	incubations	incubation	NNS
cord-104279-choywmwd	152	14	with	with	IN
cord-104279-choywmwd	152	15	goat	goat	NNP
cord-104279-choywmwd	152	16	antirabbit	antirabbit	NNP
cord-104279-choywmwd	152	17	antibody	antibody	NN
cord-104279-choywmwd	152	18	conjugated	conjugate	VBN
cord-104279-choywmwd	152	19	to	to	IN
cord-104279-choywmwd	152	20	biotin	biotin	NN
cord-104279-choywmwd	152	21	,	,	,
cord-104279-choywmwd	152	22	followed	follow	VBN
cord-104279-choywmwd	152	23	by	by	IN
cord-104279-choywmwd	152	24	a	a	DT
cord-104279-choywmwd	152	25	streptavidin	streptavidin	NN
cord-104279-choywmwd	152	26	-	-	HYPH
cord-104279-choywmwd	152	27	FITC	FITC	NNP
cord-104279-choywmwd	152	28	conjugate	conjugate	NN
cord-104279-choywmwd	152	29	.	.	.
cord-104279-choywmwd	153	1	The	the	DT
cord-104279-choywmwd	153	2	last	last	JJ
cord-104279-choywmwd	153	3	antibody	antibody	NN
cord-104279-choywmwd	153	4	incubation	incubation	NN
cord-104279-choywmwd	153	5	also	also	RB
cord-104279-choywmwd	153	6	contained	contain	VBD
cord-104279-choywmwd	153	7	goat	goat	NN
cord-104279-choywmwd	153	8	anti	anti	JJ
cord-104279-choywmwd	153	9	-	-	JJ
cord-104279-choywmwd	153	10	rabbit	rabbit	JJ
cord-104279-choywmwd	153	11	antibody	antibody	NN
cord-104279-choywmwd	153	12	conjugated	conjugate	VBN
cord-104279-choywmwd	153	13	to	to	IN
cord-104279-choywmwd	153	14	rhodamine	rhodamine	VB
cord-104279-choywmwd	153	15	.	.	.
cord-104279-choywmwd	154	1	Co	co	NN
cord-104279-choywmwd	154	2	-	-	NN
cord-104279-choywmwd	154	3	detection	detection	NN
cord-104279-choywmwd	154	4	of	of	IN
cord-104279-choywmwd	154	5	ALP	ALP	NNP
cord-104279-choywmwd	154	6	and	and	CC
cord-104279-choywmwd	154	7	Fusl	Fusl	NNP
cord-104279-choywmwd	154	8	-	-	HYPH
cord-104279-choywmwd	154	9	LacZp	LacZp	NNP
cord-104279-choywmwd	154	10	was	be	VBD
cord-104279-choywmwd	154	11	performed	perform	VBN
cord-104279-choywmwd	154	12	by	by	IN
cord-104279-choywmwd	154	13	staining	stain	VBG
cord-104279-choywmwd	154	14	cells	cell	NNS
cord-104279-choywmwd	154	15	with	with	IN
cord-104279-choywmwd	154	16	a	a	DT
cord-104279-choywmwd	154	17	1:10	1:10	CD
cord-104279-choywmwd	154	18	dilution	dilution	NN
cord-104279-choywmwd	154	19	of	of	IN
cord-104279-choywmwd	154	20	a	a	DT
cord-104279-choywmwd	154	21	rabbit	rabbit	NN
cord-104279-choywmwd	154	22	polyclonal	polyclonal	JJ
cord-104279-choywmwd	154	23	anti	anti	JJ
cord-104279-choywmwd	154	24	-	-	JJ
cord-104279-choywmwd	154	25	ALP	alp	JJ
cord-104279-choywmwd	154	26	antibody	antibody	NN
cord-104279-choywmwd	154	27	(	(	-LRB-
cord-104279-choywmwd	154	28	Raymond	Raymond	NNP
cord-104279-choywmwd	154	29	et	et	NNP
cord-104279-choywmwd	154	30	al	al	NNP
cord-104279-choywmwd	154	31	.	.	NNP
cord-104279-choywmwd	154	32	,	,	,
cord-104279-choywmwd	154	33	1990	1990	CD
cord-104279-choywmwd	154	34	)	)	-RRB-
cord-104279-choywmwd	154	35	and	and	CC
cord-104279-choywmwd	154	36	a	a	DT
cord-104279-choywmwd	154	37	1:1,000	1:1,000	CD
cord-104279-choywmwd	154	38	dilution	dilution	NN
cord-104279-choywmwd	154	39	of	of	IN
cord-104279-choywmwd	154	40	a	a	DT
cord-104279-choywmwd	154	41	mouse	mouse	NN
cord-104279-choywmwd	154	42	monoclonai	monoclonai	NNP
cord-104279-choywmwd	154	43	anti-/~-gaiactusidase	anti-/~-gaiactusidase	NNP
cord-104279-choywmwd	154	44	antibody	antibody	NN
cord-104279-choywmwd	154	45	,	,	,
cord-104279-choywmwd	154	46	followed	follow	VBN
cord-104279-choywmwd	154	47	by	by	IN
cord-104279-choywmwd	154	48	antibody	antibody	NN
cord-104279-choywmwd	154	49	amplification	amplification	NN
cord-104279-choywmwd	154	50	identical	identical	JJ
cord-104279-choywmwd	154	51	to	to	IN
cord-104279-choywmwd	154	52	that	that	DT
cord-104279-choywmwd	154	53	used	use	VBN
cord-104279-choywmwd	154	54	for	for	IN
cord-104279-choywmwd	154	55	DPAP	DPAP	NNP
cord-104279-choywmwd	154	56	A	A	NNP
cord-104279-choywmwd	154	57	and	and	CC
cord-104279-choywmwd	154	58	DPAP	DPAP	NNP
cord-104279-choywmwd	154	59	B.	B.	NNP
cord-104279-choywmwd	155	1	The	the	DT
cord-104279-choywmwd	155	2	cells	cell	NNS
cord-104279-choywmwd	155	3	were	be	VBD
cord-104279-choywmwd	155	4	mounted	mount	VBN
cord-104279-choywmwd	155	5	in	in	IN
cord-104279-choywmwd	155	6	media	medium	NNS
cord-104279-choywmwd	155	7	containing	contain	VBG
cord-104279-choywmwd	155	8	DAPI	DAPI	NNP
cord-104279-choywmwd	155	9	for	for	IN
cord-104279-choywmwd	155	10	staining	stain	VBG
cord-104279-choywmwd	155	11	nuclei	nucleus	NNS
cord-104279-choywmwd	155	12	,	,	,
cord-104279-choywmwd	155	13	and	and	CC
cord-104279-choywmwd	155	14	photomicrographs	photomicrograph	NNS
cord-104279-choywmwd	155	15	were	be	VBD
cord-104279-choywmwd	155	16	made	make	VBN
cord-104279-choywmwd	155	17	as	as	IN
cord-104279-choywmwd	155	18	described	describe	VBN
cord-104279-choywmwd	155	19	previously	previously	RB
cord-104279-choywmwd	155	20	(	(	-LRB-
cord-104279-choywmwd	155	21	Roberts	Roberts	NNP
cord-104279-choywmwd	155	22	et	et	NNP
cord-104279-choywmwd	155	23	al	al	NNP
cord-104279-choywmwd	155	24	.	.	NNP
cord-104279-choywmwd	155	25	,	,	,
cord-104279-choywmwd	155	26	1989	1989	CD
cord-104279-choywmwd	155	27	)	)	-RRB-
cord-104279-choywmwd	155	28	.	.	.
cord-104279-choywmwd	156	1	The	the	DT
cord-104279-choywmwd	156	2	role	role	NN
cord-104279-choywmwd	156	3	of	of	IN
cord-104279-choywmwd	156	4	the	the	DT
cord-104279-choywmwd	156	5	lumenal	lumenal	JJ
cord-104279-choywmwd	156	6	domain	domain	NN
cord-104279-choywmwd	156	7	in	in	IN
cord-104279-choywmwd	156	8	the	the	DT
cord-104279-choywmwd	156	9	sorting	sorting	NN
cord-104279-choywmwd	156	10	of	of	IN
cord-104279-choywmwd	156	11	DPAP	DPAP	NNP
cord-104279-choywmwd	156	12	B	B	NNP
cord-104279-choywmwd	156	13	to	to	IN
cord-104279-choywmwd	156	14	the	the	DT
cord-104279-choywmwd	156	15	vacuole	vacuole	NN
cord-104279-choywmwd	156	16	was	be	VBD
cord-104279-choywmwd	156	17	addressed	address	VBN
cord-104279-choywmwd	156	18	in	in	IN
cord-104279-choywmwd	156	19	two	two	CD
cord-104279-choywmwd	156	20	ways	way	NNS
cord-104279-choywmwd	156	21	.	.	.
cord-104279-choywmwd	157	1	First	first	RB
cord-104279-choywmwd	157	2	,	,	,
cord-104279-choywmwd	157	3	to	to	TO
cord-104279-choywmwd	157	4	determine	determine	VB
cord-104279-choywmwd	157	5	if	if	IN
cord-104279-choywmwd	157	6	the	the	DT
cord-104279-choywmwd	157	7	lumenal	lumenal	JJ
cord-104279-choywmwd	157	8	domain	domain	NN
cord-104279-choywmwd	157	9	was	be	VBD
cord-104279-choywmwd	157	10	sorted	sort	VBN
cord-104279-choywmwd	157	11	to	to	IN
cord-104279-choywmwd	157	12	the	the	DT
cord-104279-choywmwd	157	13	vacuole	vacuole	NN
cord-104279-choywmwd	157	14	when	when	WRB
cord-104279-choywmwd	157	15	expressed	express	VBN
cord-104279-choywmwd	157	16	in	in	IN
cord-104279-choywmwd	157	17	the	the	DT
cord-104279-choywmwd	157	18	secretory	secretory	JJ
cord-104279-choywmwd	157	19	pathway	pathway	NN
cord-104279-choywmwd	157	20	as	as	IN
cord-104279-choywmwd	157	21	a	a	DT
cord-104279-choywmwd	157	22	soluble	soluble	JJ
cord-104279-choywmwd	157	23	protein	protein	NN
cord-104279-choywmwd	157	24	,	,	,
cord-104279-choywmwd	157	25	a	a	DT
cord-104279-choywmwd	157	26	gene	gene	NN
cord-104279-choywmwd	157	27	fusion	fusion	NN
cord-104279-choywmwd	157	28	was	be	VBD
cord-104279-choywmwd	157	29	used	use	VBN
cord-104279-choywmwd	157	30	to	to	TO
cord-104279-choywmwd	157	31	create	create	VB
cord-104279-choywmwd	157	32	the	the	DT
cord-104279-choywmwd	157	33	protein	protein	NN
cord-104279-choywmwd	157	34	ctFss	ctfss	NN
cord-104279-choywmwd	157	35	-	-	HYPH
cord-104279-choywmwd	157	36	B	B	NNP
cord-104279-choywmwd	157	37	,	,	,
cord-104279-choywmwd	157	38	consisting	consist	VBG
cord-104279-choywmwd	157	39	of	of	IN
cord-104279-choywmwd	157	40	the	the	DT
cord-104279-choywmwd	157	41	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	157	42	ER	er	NN
cord-104279-choywmwd	157	43	-	-	HYPH
cord-104279-choywmwd	157	44	targeting	target	VBG
cord-104279-choywmwd	157	45	signal	signal	NN
cord-104279-choywmwd	157	46	sequence	sequence	NN
cord-104279-choywmwd	157	47	of	of	IN
cord-104279-choywmwd	157	48	prepro	prepro	NNP
cord-104279-choywmwd	157	49	-	-	HYPH
cord-104279-choywmwd	157	50	t~	t~	NNP
cord-104279-choywmwd	157	51	factor	factor	NN
cord-104279-choywmwd	157	52	fused	fuse	VBN
cord-104279-choywmwd	157	53	to	to	IN
cord-104279-choywmwd	157	54	the	the	DT
cord-104279-choywmwd	157	55	lumenal	lumenal	JJ
cord-104279-choywmwd	157	56	domain	domain	NN
cord-104279-choywmwd	157	57	of	of	IN
cord-104279-choywmwd	157	58	DPAP	DPAP	NNP
cord-104279-choywmwd	157	59	B	B	NNP
cord-104279-choywmwd	157	60	at	at	IN
cord-104279-choywmwd	157	61	residue	residue	NN
cord-104279-choywmwd	157	62	49	49	CD
cord-104279-choywmwd	157	63	(	(	-LRB-
cord-104279-choywmwd	157	64	Fig	fig	NN
cord-104279-choywmwd	157	65	.	.	.
cord-104279-choywmwd	157	66	1	1	CD
cord-104279-choywmwd	157	67	B	b	NN
cord-104279-choywmwd	157	68	)	)	-RRB-
cord-104279-choywmwd	157	69	.	.	.
cord-104279-choywmwd	158	1	The	the	DT
cord-104279-choywmwd	158	2	signal	signal	NN
cord-104279-choywmwd	158	3	sequence	sequence	NN
cord-104279-choywmwd	158	4	should	should	MD
cord-104279-choywmwd	158	5	direct	direct	VB
cord-104279-choywmwd	158	6	the	the	DT
cord-104279-choywmwd	158	7	translocation	translocation	NN
cord-104279-choywmwd	158	8	of	of	IN
cord-104279-choywmwd	158	9	the	the	DT
cord-104279-choywmwd	158	10	protein	protein	NN
cord-104279-choywmwd	158	11	into	into	IN
cord-104279-choywmwd	158	12	the	the	DT
cord-104279-choywmwd	158	13	ER	ER	NNP
cord-104279-choywmwd	158	14	lumen	lumen	NN
cord-104279-choywmwd	158	15	and	and	CC
cord-104279-choywmwd	158	16	be	be	VB
cord-104279-choywmwd	158	17	cleaved	cleave	VBN
cord-104279-choywmwd	158	18	,	,	,
cord-104279-choywmwd	158	19	rendering	render	VBG
cord-104279-choywmwd	158	20	the	the	DT
cord-104279-choywmwd	158	21	lumenal	lumenal	JJ
cord-104279-choywmwd	158	22	domain	domain	NN
cord-104279-choywmwd	158	23	a	a	DT
cord-104279-choywmwd	158	24	soluble	soluble	JJ
cord-104279-choywmwd	158	25	protein	protein	NN
cord-104279-choywmwd	158	26	in	in	IN
cord-104279-choywmwd	158	27	the	the	DT
cord-104279-choywmwd	158	28	secretory	secretory	JJ
cord-104279-choywmwd	158	29	Figure	figure	NN
cord-104279-choywmwd	158	30	2	2	CD
cord-104279-choywmwd	158	31	.	.	.
cord-104279-choywmwd	159	1	Immunoprecipitations	immunoprecipitation	NNS
cord-104279-choywmwd	159	2	of	of	IN
cord-104279-choywmwd	159	3	c	c	NN
cord-104279-choywmwd	159	4	~	~	NFP
cord-104279-choywmwd	159	5	FssB	FssB	NNP
cord-104279-choywmwd	159	6	from	from	IN
cord-104279-choywmwd	159	7	MNN9	MNN9	NNP
cord-104279-choywmwd	159	8	and	and	CC
cord-104279-choywmwd	159	9	mnn9	mnn9	CD
cord-104279-choywmwd	159	10	strains	strain	NNS
cord-104279-choywmwd	159	11	.	.	.
cord-104279-choywmwd	160	1	JHRY20	JHRY20	NNP
cord-104279-choywmwd	160	2	-	-	HYPH
cord-104279-choywmwd	160	3	1A	1A	NNP
cord-104279-choywmwd	160	4	(	(	-LRB-
cord-104279-choywmwd	160	5	dap2A	dap2A	NNP
cord-104279-choywmwd	160	6	MNN9	MNN9	NNP
cord-104279-choywmwd	160	7	)	)	-RRB-
cord-104279-choywmwd	160	8	or	or	CC
cord-104279-choywmwd	160	9	CJRY25	cjry25	NN
cord-104279-choywmwd	160	10	-	-	HYPH
cord-104279-choywmwd	160	11	6B	6b	NN
cord-104279-choywmwd	160	12	(	(	-LRB-
cord-104279-choywmwd	160	13	dap2A	dap2A	NNP
cord-104279-choywmwd	160	14	mnn9	mnn9	CD
cord-104279-choywmwd	160	15	)	)	-RRB-
cord-104279-choywmwd	160	16	cells	cell	NNS
cord-104279-choywmwd	160	17	were	be	VBD
cord-104279-choywmwd	160	18	labeled	label	VBN
cord-104279-choywmwd	160	19	with	with	IN
cord-104279-choywmwd	160	20	Tran3sS	tran3s	NNS
cord-104279-choywmwd	160	21	-	-	HYPH
cord-104279-choywmwd	160	22	label	label	NN
cord-104279-choywmwd	160	23	for	for	IN
cord-104279-choywmwd	160	24	15	15	CD
cord-104279-choywmwd	160	25	rain	rain	NN
cord-104279-choywmwd	160	26	and	and	CC
cord-104279-choywmwd	160	27	chased	chase	VBD
cord-104279-choywmwd	160	28	for	for	IN
cord-104279-choywmwd	160	29	60	60	CD
cord-104279-choywmwd	160	30	min	min	NN
cord-104279-choywmwd	160	31	in	in	IN
cord-104279-choywmwd	160	32	the	the	DT
cord-104279-choywmwd	160	33	presence	presence	NN
cord-104279-choywmwd	160	34	of	of	IN
cord-104279-choywmwd	160	35	50	50	CD
cord-104279-choywmwd	160	36	izg	izg	NN
cord-104279-choywmwd	160	37	/	/	SYM
cord-104279-choywmwd	160	38	mL	ml	NN
cord-104279-choywmwd	160	39	methionine	methionine	NN
cord-104279-choywmwd	160	40	and	and	CC
cord-104279-choywmwd	160	41	50/~g	50/~g	NNP
cord-104279-choywmwd	160	42	/	/	SYM
cord-104279-choywmwd	160	43	mL	ml	NN
cord-104279-choywmwd	160	44	cysteine	cysteine	NN
cord-104279-choywmwd	160	45	.	.	.
cord-104279-choywmwd	161	1	Cultures	culture	NNS
cord-104279-choywmwd	161	2	were	be	VBD
cord-104279-choywmwd	161	3	separated	separate	VBN
cord-104279-choywmwd	161	4	into	into	IN
cord-104279-choywmwd	161	5	two	two	CD
cord-104279-choywmwd	161	6	fractions	fraction	NNS
cord-104279-choywmwd	161	7	,	,	,
cord-104279-choywmwd	161	8	intracellular	intracellular	JJ
cord-104279-choywmwd	161	9	(	(	-LRB-
cord-104279-choywmwd	161	10	i.e.	i.e.	FW
cord-104279-choywmwd	161	11	,	,	,
cord-104279-choywmwd	161	12	spheroplasts	spheroplasts	NNP
cord-104279-choywmwd	161	13	)	)	-RRB-
cord-104279-choywmwd	161	14	and	and	CC
cord-104279-choywmwd	161	15	extraceUular	extraceuular	JJ
cord-104279-choywmwd	161	16	(	(	-LRB-
cord-104279-choywmwd	161	17	i.e.	i.e.	FW
cord-104279-choywmwd	161	18	,	,	,
cord-104279-choywmwd	161	19	periplasmic	periplasmic	NN
cord-104279-choywmwd	161	20	and	and	CC
cord-104279-choywmwd	161	21	media	medium	NNS
cord-104279-choywmwd	161	22	)	)	-RRB-
cord-104279-choywmwd	161	23	,	,	,
cord-104279-choywmwd	161	24	and	and	CC
cord-104279-choywmwd	161	25	immunoprecipitated	immunoprecipitate	VBN
cord-104279-choywmwd	161	26	with	with	IN
cord-104279-choywmwd	161	27	affinity	affinity	NN
cord-104279-choywmwd	161	28	purified	purify	VBN
cord-104279-choywmwd	161	29	DPAP	DPAP	NNP
cord-104279-choywmwd	161	30	B	B	NNP
cord-104279-choywmwd	161	31	antibody	antibody	NN
cord-104279-choywmwd	161	32	.	.	.
cord-104279-choywmwd	162	1	Half	half	NN
cord-104279-choywmwd	162	2	of	of	IN
cord-104279-choywmwd	162	3	each	each	DT
cord-104279-choywmwd	162	4	immunoprecipitation	immunoprecipitation	NN
cord-104279-choywmwd	162	5	sample	sample	NN
cord-104279-choywmwd	162	6	was	be	VBD
cord-104279-choywmwd	162	7	treated	treat	VBN
cord-104279-choywmwd	162	8	with	with	IN
cord-104279-choywmwd	162	9	Endo	Endo	NNP
cord-104279-choywmwd	162	10	H	H	NNP
cord-104279-choywmwd	162	11	,	,	,
cord-104279-choywmwd	162	12	and	and	CC
cord-104279-choywmwd	162	13	equal	equal	JJ
cord-104279-choywmwd	162	14	amounts	amount	NNS
cord-104279-choywmwd	162	15	were	be	VBD
cord-104279-choywmwd	162	16	analyzed	analyze	VBN
cord-104279-choywmwd	162	17	by	by	IN
cord-104279-choywmwd	162	18	SDS	SDS	NNP
cord-104279-choywmwd	162	19	-	-	HYPH
cord-104279-choywmwd	162	20	PAGE	PAGE	NNP
cord-104279-choywmwd	162	21	and	and	CC
cord-104279-choywmwd	162	22	fluorography	fluorography	NN
cord-104279-choywmwd	162	23	.	.	.
cord-104279-choywmwd	163	1	Positions	position	NNS
cord-104279-choywmwd	163	2	of	of	IN
cord-104279-choywmwd	163	3	the	the	DT
cord-104279-choywmwd	163	4	molecular	molecular	JJ
cord-104279-choywmwd	163	5	weight	weight	NN
cord-104279-choywmwd	163	6	standards	standard	NNS
cord-104279-choywmwd	163	7	are	be	VBP
cord-104279-choywmwd	163	8	indicated	indicate	VBN
cord-104279-choywmwd	163	9	(	(	-LRB-
cord-104279-choywmwd	163	10	in	in	IN
cord-104279-choywmwd	163	11	kD	kD	NNP
cord-104279-choywmwd	163	12	)	)	-RRB-
cord-104279-choywmwd	163	13	.	.	.
cord-104279-choywmwd	164	1	pathway	pathway	NNP
cord-104279-choywmwd	164	2	.	.	.
cord-104279-choywmwd	165	1	The	the	DT
cord-104279-choywmwd	165	2	construct	construct	NN
cord-104279-choywmwd	165	3	was	be	VBD
cord-104279-choywmwd	165	4	analyzed	analyze	VBN
cord-104279-choywmwd	165	5	in	in	IN
cord-104279-choywmwd	165	6	a	a	DT
cord-104279-choywmwd	165	7	strain	strain	NN
cord-104279-choywmwd	165	8	which	which	WDT
cord-104279-choywmwd	165	9	contains	contain	VBZ
cord-104279-choywmwd	165	10	a	a	DT
cord-104279-choywmwd	165	11	null	null	JJ
cord-104279-choywmwd	165	12	allele	allele	NN
cord-104279-choywmwd	165	13	of	of	IN
cord-104279-choywmwd	165	14	DAP2	DAP2	NNP
cord-104279-choywmwd	165	15	,	,	,
cord-104279-choywmwd	165	16	the	the	DT
cord-104279-choywmwd	165	17	structural	structural	JJ
cord-104279-choywmwd	165	18	gene	gene	NN
cord-104279-choywmwd	165	19	for	for	IN
cord-104279-choywmwd	165	20	DPAP	DPAP	NNP
cord-104279-choywmwd	165	21	B	B	NNP
cord-104279-choywmwd	166	1	(	(	-LRB-
cord-104279-choywmwd	166	2	Suarez	Suarez	NNP
cord-104279-choywmwd	166	3	Rendueles	Rendueles	NNP
cord-104279-choywmwd	166	4	and	and	CC
cord-104279-choywmwd	166	5	Wolf	Wolf	NNP
cord-104279-choywmwd	166	6	,	,	,
cord-104279-choywmwd	166	7	1987	1987	CD
cord-104279-choywmwd	166	8	)	)	-RRB-
cord-104279-choywmwd	166	9	.	.	.
cord-104279-choywmwd	167	1	Fig	Fig	NNP
cord-104279-choywmwd	167	2	.	.	NNP
cord-104279-choywmwd	167	3	2	2	CD
cord-104279-choywmwd	167	4	shows	show	VBZ
cord-104279-choywmwd	167	5	the	the	DT
cord-104279-choywmwd	167	6	results	result	NNS
cord-104279-choywmwd	167	7	of	of	IN
cord-104279-choywmwd	167	8	immunoprecipitating	immunoprecipitate	VBG
cord-104279-choywmwd	167	9	o	o	NN
cord-104279-choywmwd	168	1	~Fss	~fss	NN
cord-104279-choywmwd	168	2	-	-	:
cord-104279-choywmwd	168	3	B	b	NN
cord-104279-choywmwd	168	4	from	from	IN
cord-104279-choywmwd	168	5	intraceUular	intraceUular	NNP
cord-104279-choywmwd	168	6	and	and	CC
cord-104279-choywmwd	168	7	ex	ex	NN
cord-104279-choywmwd	168	8	-	-	JJ
cord-104279-choywmwd	168	9	traceUular	traceuular	JJ
cord-104279-choywmwd	168	10	(	(	-LRB-
cord-104279-choywmwd	168	11	i.e.	i.e.	FW
cord-104279-choywmwd	168	12	,	,	,
cord-104279-choywmwd	168	13	combined	combined	JJ
cord-104279-choywmwd	168	14	periplasmic	periplasmic	NN
cord-104279-choywmwd	168	15	and	and	CC
cord-104279-choywmwd	168	16	medium	medium	NN
cord-104279-choywmwd	168	17	)	)	-RRB-
cord-104279-choywmwd	168	18	fractions	fraction	NNS
cord-104279-choywmwd	168	19	of	of	IN
cord-104279-choywmwd	168	20	35S	35S	NNP
cord-104279-choywmwd	168	21	-	-	HYPH
cord-104279-choywmwd	168	22	labeled	label	VBN
cord-104279-choywmwd	168	23	cells	cell	NNS
cord-104279-choywmwd	168	24	using	use	VBG
cord-104279-choywmwd	168	25	an	an	DT
cord-104279-choywmwd	168	26	antibody	antibody	NN
cord-104279-choywmwd	168	27	that	that	WDT
cord-104279-choywmwd	168	28	recognizes	recognize	VBZ
cord-104279-choywmwd	168	29	the	the	DT
cord-104279-choywmwd	168	30	COOH	COOH	NNP
cord-104279-choywmwd	168	31	-	-	HYPH
cord-104279-choywmwd	168	32	terminal	terminal	JJ
cord-104279-choywmwd	168	33	half	half	NN
cord-104279-choywmwd	168	34	of	of	IN
cord-104279-choywmwd	168	35	DPAP	DPAP	NNP
cord-104279-choywmwd	168	36	B	B	NNP
cord-104279-choywmwd	169	1	(	(	-LRB-
cord-104279-choywmwd	169	2	Roberts	Roberts	NNP
cord-104279-choywmwd	169	3	et	et	NNP
cord-104279-choywmwd	169	4	al	al	NNP
cord-104279-choywmwd	169	5	.	.	NNP
cord-104279-choywmwd	169	6	,	,	,
cord-104279-choywmwd	169	7	1989	1989	CD
cord-104279-choywmwd	169	8	)	)	-RRB-
cord-104279-choywmwd	169	9	.	.	.
cord-104279-choywmwd	170	1	Analysis	analysis	NN
cord-104279-choywmwd	170	2	of	of	IN
cord-104279-choywmwd	170	3	the	the	DT
cord-104279-choywmwd	170	4	samples	sample	NNS
cord-104279-choywmwd	170	5	by	by	IN
cord-104279-choywmwd	170	6	SDS	SDS	NNP
cord-104279-choywmwd	170	7	-	-	HYPH
cord-104279-choywmwd	170	8	PAGE	PAGE	NNP
cord-104279-choywmwd	170	9	and	and	CC
cord-104279-choywmwd	170	10	fluorography	fluorography	NN
cord-104279-choywmwd	170	11	showed	show	VBD
cord-104279-choywmwd	170	12	that	that	IN
cord-104279-choywmwd	170	13	64	64	CD
cord-104279-choywmwd	170	14	%	%	NN
cord-104279-choywmwd	170	15	of	of	IN
cord-104279-choywmwd	170	16	txFss	txfss	NN
cord-104279-choywmwd	170	17	-	-	HYPH
cord-104279-choywmwd	170	18	B	B	NNP
cord-104279-choywmwd	170	19	was	be	VBD
cord-104279-choywmwd	170	20	secreted	secrete	VBN
cord-104279-choywmwd	170	21	as	as	IN
cord-104279-choywmwd	170	22	a	a	DT
cord-104279-choywmwd	170	23	heterogeneous	heterogeneous	JJ
cord-104279-choywmwd	170	24	population	population	NN
cord-104279-choywmwd	170	25	of	of	IN
cord-104279-choywmwd	170	26	highly	highly	RB
cord-104279-choywmwd	170	27	glycosylated	glycosylate	VBN
cord-104279-choywmwd	170	28	species	specie	NNS
cord-104279-choywmwd	170	29	(	(	-LRB-
cord-104279-choywmwd	170	30	Fig	Fig	NNP
cord-104279-choywmwd	170	31	.	.	NNP
cord-104279-choywmwd	170	32	2	2	CD
cord-104279-choywmwd	170	33	,	,	,
cord-104279-choywmwd	170	34	lane	lane	NN
cord-104279-choywmwd	170	35	2	2	CD
cord-104279-choywmwd	170	36	)	)	-RRB-
cord-104279-choywmwd	170	37	,	,	,
cord-104279-choywmwd	170	38	similar	similar	JJ
cord-104279-choywmwd	170	39	to	to	IN
cord-104279-choywmwd	170	40	the	the	DT
cord-104279-choywmwd	170	41	secreted	secrete	VBN
cord-104279-choywmwd	170	42	protein	protein	NN
cord-104279-choywmwd	170	43	invertase	invertase	NN
cord-104279-choywmwd	171	1	(	(	-LRB-
cord-104279-choywmwd	171	2	Esmon	Esmon	NNP
cord-104279-choywmwd	171	3	et	et	NNP
cord-104279-choywmwd	171	4	al	al	NNP
cord-104279-choywmwd	171	5	.	.	NNP
cord-104279-choywmwd	171	6	,	,	,
cord-104279-choywmwd	171	7	1981	1981	CD
cord-104279-choywmwd	171	8	)	)	-RRB-
cord-104279-choywmwd	171	9	,	,	,
cord-104279-choywmwd	171	10	whereas	whereas	IN
cord-104279-choywmwd	171	11	the	the	DT
cord-104279-choywmwd	171	12	intracellular	intracellular	JJ
cord-104279-choywmwd	171	13	fraction	fraction	NN
cord-104279-choywmwd	171	14	contained	contain	VBD
cord-104279-choywmwd	171	15	a	a	DT
cord-104279-choywmwd	171	16	tightly	tightly	RB
cord-104279-choywmwd	171	17	migrating	migrate	VBG
cord-104279-choywmwd	171	18	species	specie	NNS
cord-104279-choywmwd	171	19	(	(	-LRB-
cord-104279-choywmwd	171	20	Fig	Fig	NNP
cord-104279-choywmwd	171	21	.	.	NNP
cord-104279-choywmwd	171	22	2	2	CD
cord-104279-choywmwd	171	23	,	,	,
cord-104279-choywmwd	171	24	lane	lane	NN
cord-104279-choywmwd	171	25	/	/	SYM
cord-104279-choywmwd	171	26	)	)	-RRB-
cord-104279-choywmwd	171	27	.	.	.
cord-104279-choywmwd	172	1	Treatment	treatment	NN
cord-104279-choywmwd	172	2	of	of	IN
cord-104279-choywmwd	172	3	the	the	DT
cord-104279-choywmwd	172	4	immunoprecipitates	immunoprecipitate	NNS
cord-104279-choywmwd	172	5	with	with	IN
cord-104279-choywmwd	172	6	Endo	Endo	NNP
cord-104279-choywmwd	172	7	H	H	NNP
cord-104279-choywmwd	172	8	to	to	TO
cord-104279-choywmwd	172	9	remove	remove	VB
cord-104279-choywmwd	172	10	N	n	CD
cord-104279-choywmwd	172	11	-	-	HYPH
cord-104279-choywmwd	172	12	linked	link	VBN
cord-104279-choywmwd	172	13	carbohydrate	carbohydrate	NN
cord-104279-choywmwd	172	14	demonstrated	demonstrate	VBD
cord-104279-choywmwd	172	15	that	that	IN
cord-104279-choywmwd	172	16	the	the	DT
cord-104279-choywmwd	172	17	difference	difference	NN
cord-104279-choywmwd	172	18	in	in	IN
cord-104279-choywmwd	172	19	the	the	DT
cord-104279-choywmwd	172	20	apparent	apparent	JJ
cord-104279-choywmwd	172	21	mobilities	mobility	NNS
cord-104279-choywmwd	172	22	was	be	VBD
cord-104279-choywmwd	172	23	due	due	JJ
cord-104279-choywmwd	172	24	to	to	IN
cord-104279-choywmwd	172	25	glycosylation	glycosylation	NN
cord-104279-choywmwd	173	1	(	(	-LRB-
cord-104279-choywmwd	173	2	Fig	Fig	NNP
cord-104279-choywmwd	173	3	.	.	NNP
cord-104279-choywmwd	173	4	2	2	CD
cord-104279-choywmwd	173	5	,	,	,
cord-104279-choywmwd	173	6	lanes	lane	NNS
cord-104279-choywmwd	173	7	3	3	CD
cord-104279-choywmwd	173	8	and	and	CC
cord-104279-choywmwd	173	9	4	4	CD
cord-104279-choywmwd	173	10	)	)	-RRB-
cord-104279-choywmwd	173	11	.	.	.
cord-104279-choywmwd	174	1	The	the	DT
cord-104279-choywmwd	174	2	glycosylation	glycosylation	NN
cord-104279-choywmwd	174	3	pattern	pattern	NN
cord-104279-choywmwd	174	4	of	of	IN
cord-104279-choywmwd	174	5	the	the	DT
cord-104279-choywmwd	174	6	secreted	secrete	VBN
cord-104279-choywmwd	174	7	material	material	NN
cord-104279-choywmwd	174	8	differs	differ	VBZ
cord-104279-choywmwd	174	9	from	from	IN
cord-104279-choywmwd	174	10	that	that	DT
cord-104279-choywmwd	174	11	of	of	IN
cord-104279-choywmwd	174	12	wild	wild	JJ
cord-104279-choywmwd	174	13	type	type	NN
cord-104279-choywmwd	174	14	DPAP	DPAP	NNP
cord-104279-choywmwd	174	15	B	B	NNP
cord-104279-choywmwd	174	16	,	,	,
cord-104279-choywmwd	174	17	which	which	WDT
cord-104279-choywmwd	174	18	receives	receive	VBZ
cord-104279-choywmwd	174	19	only	only	RB
cord-104279-choywmwd	174	20	modest	modest	JJ
cord-104279-choywmwd	174	21	glycosyl	glycosyl	JJ
cord-104279-choywmwd	174	22	modifications	modification	NNS
cord-104279-choywmwd	174	23	of	of	IN
cord-104279-choywmwd	174	24	the	the	DT
cord-104279-choywmwd	174	25	core	core	NN
cord-104279-choywmwd	174	26	oligosaccharides	oligosaccharide	NNS
cord-104279-choywmwd	174	27	in	in	IN
cord-104279-choywmwd	174	28	the	the	DT
cord-104279-choywmwd	174	29	Golgi	Golgi	NNP
cord-104279-choywmwd	174	30	apparatus	apparatus	NN
cord-104279-choywmwd	174	31	(	(	-LRB-
cord-104279-choywmwd	174	32	Roberts	Roberts	NNP
cord-104279-choywmwd	174	33	et	et	NNP
cord-104279-choywmwd	174	34	al	al	NNP
cord-104279-choywmwd	174	35	.	.	NNP
cord-104279-choywmwd	174	36	,	,	,
cord-104279-choywmwd	174	37	1989	1989	CD
cord-104279-choywmwd	174	38	)	)	-RRB-
cord-104279-choywmwd	174	39	.	.	.
cord-104279-choywmwd	175	1	To	to	TO
cord-104279-choywmwd	175	2	test	test	VB
cord-104279-choywmwd	175	3	whether	whether	IN
cord-104279-choywmwd	175	4	the	the	DT
cord-104279-choywmwd	175	5	alteration	alteration	NN
cord-104279-choywmwd	175	6	in	in	IN
cord-104279-choywmwd	175	7	glycosylation	glycosylation	NN
cord-104279-choywmwd	175	8	caused	cause	VBD
cord-104279-choywmwd	175	9	the	the	DT
cord-104279-choywmwd	175	10	secretion	secretion	NN
cord-104279-choywmwd	175	11	of	of	IN
cord-104279-choywmwd	175	12	the	the	DT
cord-104279-choywmwd	175	13	lumenal	lumenal	JJ
cord-104279-choywmwd	175	14	domain	domain	NN
cord-104279-choywmwd	175	15	,	,	,
cord-104279-choywmwd	175	16	otFss	otfss	NN
cord-104279-choywmwd	175	17	-	-	HYPH
cord-104279-choywmwd	175	18	B	B	NNP
cord-104279-choywmwd	175	19	was	be	VBD
cord-104279-choywmwd	175	20	expressed	express	VBN
cord-104279-choywmwd	175	21	in	in	IN
cord-104279-choywmwd	175	22	an	an	DT
cord-104279-choywmwd	175	23	mnn9	mnn9	CD
cord-104279-choywmwd	175	24	mutant	mutant	NN
cord-104279-choywmwd	175	25	,	,	,
cord-104279-choywmwd	175	26	which	which	WDT
cord-104279-choywmwd	175	27	is	be	VBZ
cord-104279-choywmwd	175	28	deficient	deficient	JJ
cord-104279-choywmwd	175	29	in	in	IN
cord-104279-choywmwd	175	30	the	the	DT
cord-104279-choywmwd	175	31	addition	addition	NN
cord-104279-choywmwd	175	32	of	of	IN
cord-104279-choywmwd	175	33	the	the	DT
cord-104279-choywmwd	175	34	extensive	extensive	JJ
cord-104279-choywmwd	175	35	t~1,6	t~1,6	NNP
cord-104279-choywmwd	175	36	outer	outer	JJ
cord-104279-choywmwd	175	37	chain	chain	NN
cord-104279-choywmwd	175	38	glycosyl	glycosyl	NN
cord-104279-choywmwd	175	39	groups	group	NNS
cord-104279-choywmwd	175	40	(	(	-LRB-
cord-104279-choywmwd	175	41	Kukuruzinska	Kukuruzinska	NNP
cord-104279-choywmwd	175	42	et	et	FW
cord-104279-choywmwd	175	43	al	al	NNP
cord-104279-choywmwd	175	44	.	.	NNP
cord-104279-choywmwd	175	45	,	,	,
cord-104279-choywmwd	175	46	1987	1987	CD
cord-104279-choywmwd	175	47	)	)	-RRB-
cord-104279-choywmwd	175	48	.	.	.
cord-104279-choywmwd	176	1	Fig	Fig	NNP
cord-104279-choywmwd	176	2	.	.	NNP
cord-104279-choywmwd	176	3	2	2	CD
cord-104279-choywmwd	176	4	,	,	,
cord-104279-choywmwd	176	5	lanes	lane	NNS
cord-104279-choywmwd	176	6	5	5	CD
cord-104279-choywmwd	176	7	and	and	CC
cord-104279-choywmwd	176	8	6	6	CD
cord-104279-choywmwd	176	9	,	,	,
cord-104279-choywmwd	176	10	show	show	VBP
cord-104279-choywmwd	176	11	that	that	IN
cord-104279-choywmwd	176	12	t	t	NN
cord-104279-choywmwd	176	13	~	~	CD
cord-104279-choywmwd	176	14	Fss	Fss	NNP
cord-104279-choywmwd	176	15	-	-	HYPH
cord-104279-choywmwd	176	16	B	B	NNP
cord-104279-choywmwd	176	17	was	be	VBD
cord-104279-choywmwd	176	18	secreted	secrete	VBN
cord-104279-choywmwd	176	19	to	to	IN
cord-104279-choywmwd	176	20	the	the	DT
cord-104279-choywmwd	176	21	same	same	JJ
cord-104279-choywmwd	176	22	extent	extent	NN
cord-104279-choywmwd	176	23	(	(	-LRB-
cord-104279-choywmwd	176	24	59	59	CD
cord-104279-choywmwd	176	25	%	%	NN
cord-104279-choywmwd	176	26	)	)	-RRB-
cord-104279-choywmwd	176	27	from	from	IN
cord-104279-choywmwd	176	28	mnn9	mnn9	CD
cord-104279-choywmwd	176	29	cells	cell	NNS
cord-104279-choywmwd	176	30	,	,	,
cord-104279-choywmwd	176	31	even	even	RB
cord-104279-choywmwd	176	32	though	though	IN
cord-104279-choywmwd	176	33	the	the	DT
cord-104279-choywmwd	176	34	protein	protein	NN
cord-104279-choywmwd	176	35	was	be	VBD
cord-104279-choywmwd	176	36	not	not	RB
cord-104279-choywmwd	176	37	aberrantly	aberrantly	RB
cord-104279-choywmwd	176	38	glycosylated	glycosylate	VBN
cord-104279-choywmwd	176	39	.	.	.
cord-104279-choywmwd	177	1	Indirect	indirect	JJ
cord-104279-choywmwd	177	2	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	177	3	microscopy	microscopy	NNS
cord-104279-choywmwd	177	4	showed	show	VBD
cord-104279-choywmwd	177	5	that	that	IN
cord-104279-choywmwd	177	6	the	the	DT
cord-104279-choywmwd	177	7	portion	portion	NN
cord-104279-choywmwd	177	8	of	of	IN
cord-104279-choywmwd	177	9	the	the	DT
cord-104279-choywmwd	177	10	lumenal	lumenal	JJ
cord-104279-choywmwd	177	11	domain	domain	NN
cord-104279-choywmwd	177	12	that	that	WDT
cord-104279-choywmwd	177	13	remained	remain	VBD
cord-104279-choywmwd	177	14	intracellular	intracellular	JJ
cord-104279-choywmwd	177	15	was	be	VBD
cord-104279-choywmwd	177	16	retained	retain	VBN
cord-104279-choywmwd	177	17	in	in	IN
cord-104279-choywmwd	177	18	the	the	DT
cord-104279-choywmwd	177	19	ER	ER	NNP
cord-104279-choywmwd	177	20	,	,	,
cord-104279-choywmwd	177	21	and	and	CC
cord-104279-choywmwd	177	22	no	no	DT
cord-104279-choywmwd	177	23	staining	staining	NN
cord-104279-choywmwd	177	24	of	of	IN
cord-104279-choywmwd	177	25	the	the	DT
cord-104279-choywmwd	177	26	vacuole	vacuole	NN
cord-104279-choywmwd	177	27	was	be	VBD
cord-104279-choywmwd	177	28	observed	observe	VBN
cord-104279-choywmwd	177	29	(	(	-LRB-
cord-104279-choywmwd	177	30	data	datum	NNS
cord-104279-choywmwd	177	31	not	not	RB
cord-104279-choywmwd	177	32	shown	show	VBN
cord-104279-choywmwd	177	33	)	)	-RRB-
cord-104279-choywmwd	177	34	.	.	.
cord-104279-choywmwd	178	1	Unlike	unlike	IN
cord-104279-choywmwd	178	2	the	the	DT
cord-104279-choywmwd	178	3	secreted	secrete	VBN
cord-104279-choywmwd	178	4	material	material	NN
cord-104279-choywmwd	178	5	,	,	,
cord-104279-choywmwd	178	6	the	the	DT
cord-104279-choywmwd	178	7	ER	ER	NNP
cord-104279-choywmwd	178	8	-	-	HYPH
cord-104279-choywmwd	178	9	retalned	retalned	JJ
cord-104279-choywmwd	178	10	material	material	NN
cord-104279-choywmwd	178	11	was	be	VBD
cord-104279-choywmwd	178	12	enzymati-	enzymati-	JJ
cord-104279-choywmwd	179	1	(	(	-LRB-
cord-104279-choywmwd	179	2	suc2-A9	suc2-A9	NNP
cord-104279-choywmwd	179	3	)	)	-RRB-
cord-104279-choywmwd	179	4	cells	cell	NNS
cord-104279-choywmwd	179	5	containing	contain	VBG
cord-104279-choywmwd	179	6	a	a	DT
cord-104279-choywmwd	179	7	plasmid	plasmid	NN
cord-104279-choywmwd	179	8	encoding	encode	VBG
cord-104279-choywmwd	179	9	BB	bb	NN
cord-104279-choywmwd	179	10	-	-	HYPH
cord-104279-choywmwd	179	11	Inv	Inv	NNP
cord-104279-choywmwd	179	12	(	(	-LRB-
cord-104279-choywmwd	179	13	pCJR13	pcjr13	NN
cord-104279-choywmwd	179	14	)	)	-RRB-
cord-104279-choywmwd	179	15	were	be	VBD
cord-104279-choywmwd	179	16	labeled	label	VBN
cord-104279-choywmwd	179	17	with	with	IN
cord-104279-choywmwd	179	18	Tran35S	tran35s	NN
cord-104279-choywmwd	179	19	-	-	NN
cord-104279-choywmwd	179	20	label	label	NN
cord-104279-choywmwd	179	21	for	for	IN
cord-104279-choywmwd	179	22	30	30	CD
cord-104279-choywmwd	179	23	rain	rain	NN
cord-104279-choywmwd	179	24	and	and	CC
cord-104279-choywmwd	179	25	chased	chase	VBN
cord-104279-choywmwd	179	26	for	for	IN
cord-104279-choywmwd	179	27	60	60	CD
cord-104279-choywmwd	179	28	rain	rain	NN
cord-104279-choywmwd	179	29	in	in	IN
cord-104279-choywmwd	179	30	the	the	DT
cord-104279-choywmwd	179	31	presence	presence	NN
cord-104279-choywmwd	179	32	of	of	IN
cord-104279-choywmwd	179	33	50/~g	50/~g	NNP
cord-104279-choywmwd	179	34	/	/	SYM
cord-104279-choywmwd	179	35	mL	ml	NN
cord-104279-choywmwd	179	36	rrw	rrw	NN
cord-104279-choywmwd	179	37	,	,	,
cord-104279-choywmwd	179	38	thionine	thionine	NN
cord-104279-choywmwd	179	39	and	and	CC
cord-104279-choywmwd	179	40	50	50	CD
cord-104279-choywmwd	179	41	/~g	/~g	,
cord-104279-choywmwd	179	42	/	/	SYM
cord-104279-choywmwd	179	43	ml	ml	NN
cord-104279-choywmwd	179	44	cysteine	cysteine	NN
cord-104279-choywmwd	179	45	.	.	.
cord-104279-choywmwd	180	1	The	the	DT
cord-104279-choywmwd	180	2	cells	cell	NNS
cord-104279-choywmwd	180	3	were	be	VBD
cord-104279-choywmwd	180	4	converted	convert	VBN
cord-104279-choywmwd	180	5	to	to	IN
cord-104279-choywmwd	180	6	sphemplasts	sphemplast	NNS
cord-104279-choywmwd	180	7	,	,	,
cord-104279-choywmwd	180	8	and	and	CC
cord-104279-choywmwd	180	9	extracts	extract	NNS
cord-104279-choywmwd	180	10	immunoprecipitated	immunoprecipitate	VBN
cord-104279-choywmwd	180	11	with	with	IN
cord-104279-choywmwd	180	12	either	either	CC
cord-104279-choywmwd	180	13	affinity	affinity	NN
cord-104279-choywmwd	180	14	-	-	HYPH
cord-104279-choywmwd	180	15	purified	purify	VBN
cord-104279-choywmwd	180	16	DPAP	DPAP	NNP
cord-104279-choywmwd	180	17	B	B	NNP
cord-104279-choywmwd	180	18	antibody	antibody	NN
cord-104279-choywmwd	180	19	(	(	-LRB-
cord-104279-choywmwd	180	20	lanes	lane	NNS
cord-104279-choywmwd	180	21	1	1	CD
cord-104279-choywmwd	180	22	-	-	SYM
cord-104279-choywmwd	180	23	8	8	CD
cord-104279-choywmwd	180	24	)	)	-RRB-
cord-104279-choywmwd	180	25	or	or	CC
cord-104279-choywmwd	180	26	affinity	affinity	NN
cord-104279-choywmwd	180	27	-	-	HYPH
cord-104279-choywmwd	180	28	purified	purify	VBN
cord-104279-choywmwd	180	29	invertase	invertase	NNP
cord-104279-choywmwd	180	30	antibody	antibody	NN
cord-104279-choywmwd	180	31	(	(	-LRB-
cord-104279-choywmwd	180	32	lanes	lane	NNS
cord-104279-choywmwd	180	33	9	9	CD
cord-104279-choywmwd	180	34	and	and	CC
cord-104279-choywmwd	180	35	10	10	CD
cord-104279-choywmwd	180	36	)	)	-RRB-
cord-104279-choywmwd	180	37	.	.	.
cord-104279-choywmwd	181	1	Half	half	NN
cord-104279-choywmwd	181	2	of	of	IN
cord-104279-choywmwd	181	3	each	each	DT
cord-104279-choywmwd	181	4	immunoprecipitation	immunoprecipitation	NN
cord-104279-choywmwd	181	5	sample	sample	NN
cord-104279-choywmwd	181	6	was	be	VBD
cord-104279-choywmwd	181	7	treated	treat	VBN
cord-104279-choywmwd	181	8	with	with	IN
cord-104279-choywmwd	181	9	Endo	Endo	NNP
cord-104279-choywmwd	181	10	H	H	NNP
cord-104279-choywmwd	181	11	,	,	,
cord-104279-choywmwd	181	12	and	and	CC
cord-104279-choywmwd	181	13	equal	equal	JJ
cord-104279-choywmwd	181	14	amounts	amount	NNS
cord-104279-choywmwd	181	15	were	be	VBD
cord-104279-choywmwd	181	16	analyzed	analyze	VBN
cord-104279-choywmwd	181	17	by	by	IN
cord-104279-choywmwd	181	18	SDS	SDS	NNP
cord-104279-choywmwd	181	19	-	-	HYPH
cord-104279-choywmwd	181	20	PAGE	PAGE	NNP
cord-104279-choywmwd	181	21	and	and	CC
cord-104279-choywmwd	181	22	fluorography	fluorography	NN
cord-104279-choywmwd	181	23	.	.	.
cord-104279-choywmwd	182	1	Positions	position	NNS
cord-104279-choywmwd	182	2	of	of	IN
cord-104279-choywmwd	182	3	the	the	DT
cord-104279-choywmwd	182	4	molecular	molecular	JJ
cord-104279-choywmwd	182	5	mass	mass	NN
cord-104279-choywmwd	182	6	standards	standard	NNS
cord-104279-choywmwd	182	7	for	for	IN
cord-104279-choywmwd	182	8	lanes	lane	NNS
cord-104279-choywmwd	182	9	1	1	CD
cord-104279-choywmwd	182	10	-	-	SYM
cord-104279-choywmwd	182	11	8	8	CD
cord-104279-choywmwd	182	12	are	be	VBP
cord-104279-choywmwd	182	13	indicated	indicate	VBN
cord-104279-choywmwd	182	14	(	(	-LRB-
cord-104279-choywmwd	182	15	in	in	IN
cord-104279-choywmwd	182	16	kD	kD	NNP
cord-104279-choywmwd	182	17	)	)	-RRB-
cord-104279-choywmwd	182	18	on	on	IN
cord-104279-choywmwd	182	19	the	the	DT
cord-104279-choywmwd	182	20	left	left	NN
cord-104279-choywmwd	182	21	,	,	,
cord-104279-choywmwd	182	22	whereas	whereas	IN
cord-104279-choywmwd	182	23	standards	standard	NNS
cord-104279-choywmwd	182	24	for	for	IN
cord-104279-choywmwd	182	25	lanes	lane	NNS
cord-104279-choywmwd	182	26	9	9	CD
cord-104279-choywmwd	182	27	and	and	CC
cord-104279-choywmwd	182	28	10	10	CD
cord-104279-choywmwd	182	29	are	be	VBP
cord-104279-choywmwd	182	30	indicated	indicate	VBN
cord-104279-choywmwd	182	31	on	on	IN
cord-104279-choywmwd	182	32	the	the	DT
cord-104279-choywmwd	182	33	right	right	NN
cord-104279-choywmwd	182	34	.	.	.
cord-104279-choywmwd	183	1	cally	cally	RB
cord-104279-choywmwd	183	2	inactive	inactive	JJ
cord-104279-choywmwd	183	3	(	(	-LRB-
cord-104279-choywmwd	183	4	data	datum	NNS
cord-104279-choywmwd	183	5	not	not	RB
cord-104279-choywmwd	183	6	shown	show	VBN
cord-104279-choywmwd	183	7	)	)	-RRB-
cord-104279-choywmwd	183	8	,	,	,
cord-104279-choywmwd	183	9	and	and	CC
cord-104279-choywmwd	183	10	thus	thus	RB
cord-104279-choywmwd	183	11	may	may	MD
cord-104279-choywmwd	183	12	be	be	VB
cord-104279-choywmwd	183	13	unfolded	unfold	VBN
cord-104279-choywmwd	183	14	and	and	CC
cord-104279-choywmwd	183	15	incompetent	incompetent	JJ
cord-104279-choywmwd	183	16	to	to	TO
cord-104279-choywmwd	183	17	exit	exit	VB
cord-104279-choywmwd	183	18	the	the	DT
cord-104279-choywmwd	183	19	ER	er	NN
cord-104279-choywmwd	183	20	(	(	-LRB-
cord-104279-choywmwd	183	21	Rose	Rose	NNP
cord-104279-choywmwd	183	22	et	et	FW
cord-104279-choywmwd	183	23	al	al	NNP
cord-104279-choywmwd	183	24	.	.	NNP
cord-104279-choywmwd	183	25	,	,	,
cord-104279-choywmwd	183	26	1989	1989	CD
cord-104279-choywmwd	183	27	;	;	:
cord-104279-choywmwd	183	28	Rothman	Rothman	NNP
cord-104279-choywmwd	183	29	,	,	,
cord-104279-choywmwd	183	30	1989	1989	CD
cord-104279-choywmwd	183	31	)	)	-RRB-
cord-104279-choywmwd	183	32	.	.	.
cord-104279-choywmwd	184	1	The	the	DT
cord-104279-choywmwd	184	2	role	role	NN
cord-104279-choywmwd	184	3	of	of	IN
cord-104279-choywmwd	184	4	the	the	DT
cord-104279-choywmwd	184	5	lumenal	lumenal	JJ
cord-104279-choywmwd	184	6	domain	domain	NN
cord-104279-choywmwd	184	7	of	of	IN
cord-104279-choywmwd	184	8	DPAP	DPAP	NNP
cord-104279-choywmwd	184	9	B	B	NNP
cord-104279-choywmwd	184	10	in	in	IN
cord-104279-choywmwd	184	11	vacuolar	vacuolar	NNP
cord-104279-choywmwd	184	12	targeting	targeting	NN
cord-104279-choywmwd	184	13	was	be	VBD
cord-104279-choywmwd	184	14	also	also	RB
cord-104279-choywmwd	184	15	tested	test	VBN
cord-104279-choywmwd	184	16	by	by	IN
cord-104279-choywmwd	184	17	constructing	construct	VBG
cord-104279-choywmwd	184	18	the	the	DT
cord-104279-choywmwd	184	19	fusion	fusion	NN
cord-104279-choywmwd	184	20	protein	protein	NN
cord-104279-choywmwd	184	21	BB	BB	NNP
cord-104279-choywmwd	184	22	-	-	HYPH
cord-104279-choywmwd	184	23	Inv	Inv	NNP
cord-104279-choywmwd	184	24	,	,	,
cord-104279-choywmwd	184	25	in	in	IN
cord-104279-choywmwd	184	26	which	which	WDT
cord-104279-choywmwd	184	27	the	the	DT
cord-104279-choywmwd	184	28	48	48	CD
cord-104279-choywmwd	184	29	NH2-terminal	nh2-terminal	JJ
cord-104279-choywmwd	184	30	residues	residue	NNS
cord-104279-choywmwd	184	31	of	of	IN
cord-104279-choywmwd	184	32	DPAP	DPAP	NNP
cord-104279-choywmwd	184	33	B	B	NNP
cord-104279-choywmwd	184	34	were	be	VBD
cord-104279-choywmwd	184	35	fused	fuse	VBN
cord-104279-choywmwd	184	36	to	to	IN
cord-104279-choywmwd	184	37	the	the	DT
cord-104279-choywmwd	184	38	non	non	JJ
cord-104279-choywmwd	184	39	-	-	JJ
cord-104279-choywmwd	184	40	vacuolar	vacuolar	JJ
cord-104279-choywmwd	184	41	protein	protein	NN
cord-104279-choywmwd	184	42	,	,	,
cord-104279-choywmwd	184	43	invertase	invertase	NNP
cord-104279-choywmwd	184	44	(	(	-LRB-
cord-104279-choywmwd	184	45	Fig	Fig	NNP
cord-104279-choywmwd	184	46	.	.	.
cord-104279-choywmwd	184	47	1	1	CD
cord-104279-choywmwd	184	48	B	b	NN
cord-104279-choywmwd	184	49	)	)	-RRB-
cord-104279-choywmwd	184	50	.	.	.
cord-104279-choywmwd	185	1	Immunoprecipitations	Immunoprecipitations	NNP
cord-104279-choywmwd	185	2	of	of	IN
cord-104279-choywmwd	185	3	BB	BB	NNP
cord-104279-choywmwd	185	4	-	-	HYPH
cord-104279-choywmwd	185	5	Inv	Inv	NNP
cord-104279-choywmwd	185	6	using	use	VBG
cord-104279-choywmwd	185	7	anti	anti	JJ
cord-104279-choywmwd	185	8	-	-	JJ
cord-104279-choywmwd	185	9	invertase	invertase	JJ
cord-104279-choywmwd	185	10	antibody	antibody	NN
cord-104279-choywmwd	185	11	,	,	,
cord-104279-choywmwd	185	12	followed	follow	VBN
cord-104279-choywmwd	185	13	by	by	IN
cord-104279-choywmwd	185	14	SDS	SDS	NNP
cord-104279-choywmwd	185	15	-	-	HYPH
cord-104279-choywmwd	185	16	PAGE	PAGE	NNP
cord-104279-choywmwd	185	17	and	and	CC
cord-104279-choywmwd	185	18	fluorography	fluorography	NNP
cord-104279-choywmwd	185	19	,	,	,
cord-104279-choywmwd	185	20	showed	show	VBD
cord-104279-choywmwd	185	21	that	that	IN
cord-104279-choywmwd	185	22	the	the	DT
cord-104279-choywmwd	185	23	fusion	fusion	NN
cord-104279-choywmwd	185	24	protein	protein	NN
cord-104279-choywmwd	185	25	was	be	VBD
cord-104279-choywmwd	185	26	glycosylated	glycosylate	VBN
cord-104279-choywmwd	185	27	,	,	,
cord-104279-choywmwd	185	28	and	and	CC
cord-104279-choywmwd	185	29	treatment	treatment	NN
cord-104279-choywmwd	185	30	with	with	IN
cord-104279-choywmwd	185	31	Endo	Endo	NNP
cord-104279-choywmwd	185	32	H	H	NNP
cord-104279-choywmwd	185	33	showed	show	VBD
cord-104279-choywmwd	185	34	that	that	IN
cord-104279-choywmwd	185	35	the	the	DT
cord-104279-choywmwd	185	36	protein	protein	NN
cord-104279-choywmwd	185	37	was	be	VBD
cord-104279-choywmwd	185	38	of	of	IN
cord-104279-choywmwd	185	39	the	the	DT
cord-104279-choywmwd	185	40	expected	expect	VBN
cord-104279-choywmwd	185	41	size	size	NN
cord-104279-choywmwd	185	42	(	(	-LRB-
cord-104279-choywmwd	185	43	Fig	Fig	NNP
cord-104279-choywmwd	185	44	.	.	.
cord-104279-choywmwd	185	45	3	3	CD
cord-104279-choywmwd	185	46	,	,	,
cord-104279-choywmwd	185	47	 	 	_SP
cord-104279-choywmwd	185	48	lanes	lane	NNS
cord-104279-choywmwd	185	49	9	9	CD
cord-104279-choywmwd	185	50	and	and	CC
cord-104279-choywmwd	185	51	10	10	CD
cord-104279-choywmwd	185	52	)	)	-RRB-
cord-104279-choywmwd	185	53	.	.	.
cord-104279-choywmwd	186	1	BB	BB	NNP
cord-104279-choywmwd	186	2	-	-	HYPH
cord-104279-choywmwd	186	3	Inv	inv	NN
cord-104279-choywmwd	186	4	fractionated	fractionate	VBN
cord-104279-choywmwd	186	5	with	with	IN
cord-104279-choywmwd	186	6	membranes	membrane	NNS
cord-104279-choywmwd	186	7	under	under	IN
cord-104279-choywmwd	186	8	high	high	JJ
cord-104279-choywmwd	186	9	pH	ph	NN
cord-104279-choywmwd	186	10	carbonate	carbonate	NN
cord-104279-choywmwd	186	11	conditions	condition	NNS
cord-104279-choywmwd	186	12	,	,	,
cord-104279-choywmwd	186	13	consistent	consistent	JJ
cord-104279-choywmwd	186	14	with	with	IN
cord-104279-choywmwd	186	15	BB	BB	NNP
cord-104279-choywmwd	186	16	-	-	HYPH
cord-104279-choywmwd	186	17	Inv	Inv	NNP
cord-104279-choywmwd	186	18	being	be	VBG
cord-104279-choywmwd	186	19	an	an	DT
cord-104279-choywmwd	186	20	integral	integral	JJ
cord-104279-choywmwd	186	21	membrane	membrane	NN
cord-104279-choywmwd	186	22	protein	protein	NN
cord-104279-choywmwd	186	23	(	(	-LRB-
cord-104279-choywmwd	186	24	data	datum	NNS
cord-104279-choywmwd	186	25	not	not	RB
cord-104279-choywmwd	186	26	shown	show	VBN
cord-104279-choywmwd	186	27	)	)	-RRB-
cord-104279-choywmwd	186	28	.	.	.
cord-104279-choywmwd	187	1	Invertase	Invertase	NNP
cord-104279-choywmwd	187	2	enzyme	enzyme	NN
cord-104279-choywmwd	187	3	assays	assay	NNS
cord-104279-choywmwd	187	4	of	of	IN
cord-104279-choywmwd	187	5	either	either	CC
cord-104279-choywmwd	187	6	permeabilized	permeabilized	JJ
cord-104279-choywmwd	187	7	or	or	CC
cord-104279-choywmwd	187	8	non	non	JJ
cord-104279-choywmwd	187	9	-	-	JJ
cord-104279-choywmwd	187	10	permeabilized	permeabilized	JJ
cord-104279-choywmwd	187	11	whole	whole	JJ
cord-104279-choywmwd	187	12	cells	cell	NNS
cord-104279-choywmwd	187	13	deleted	delete	VBN
cord-104279-choywmwd	187	14	for	for	IN
cord-104279-choywmwd	187	15	the	the	DT
cord-104279-choywmwd	187	16	invertase	invertase	JJ
cord-104279-choywmwd	187	17	structural	structural	JJ
cord-104279-choywmwd	187	18	gene	gene	NN
cord-104279-choywmwd	187	19	and	and	CC
cord-104279-choywmwd	187	20	expressing	express	VBG
cord-104279-choywmwd	187	21	BB	BB	NNP
cord-104279-choywmwd	187	22	-	-	HYPH
cord-104279-choywmwd	187	23	Inv	Inv	NNP
cord-104279-choywmwd	187	24	from	from	IN
cord-104279-choywmwd	187	25	a	a	DT
cord-104279-choywmwd	187	26	CEN	CEN	NNP
cord-104279-choywmwd	187	27	plasmid	plasmid	NN
cord-104279-choywmwd	187	28	showed	show	VBD
cord-104279-choywmwd	187	29	that	that	IN
cord-104279-choywmwd	187	30	<	<	XX
cord-104279-choywmwd	187	31	2	2	CD
cord-104279-choywmwd	187	32	%	%	NN
cord-104279-choywmwd	187	33	of	of	IN
cord-104279-choywmwd	187	34	the	the	DT
cord-104279-choywmwd	187	35	total	total	JJ
cord-104279-choywmwd	187	36	invertase	invertase	JJ
cord-104279-choywmwd	187	37	activity	activity	NN
cord-104279-choywmwd	187	38	was	be	VBD
cord-104279-choywmwd	187	39	extracellular	extracellular	JJ
cord-104279-choywmwd	187	40	.	.	.
cord-104279-choywmwd	188	1	The	the	DT
cord-104279-choywmwd	188	2	subcellular	subcellular	JJ
cord-104279-choywmwd	188	3	localization	localization	NN
cord-104279-choywmwd	188	4	of	of	IN
cord-104279-choywmwd	188	5	BB	BB	NNP
cord-104279-choywmwd	188	6	-	-	HYPH
cord-104279-choywmwd	188	7	Inv	Inv	NNP
cord-104279-choywmwd	188	8	was	be	VBD
cord-104279-choywmwd	188	9	determined	determine	VBN
cord-104279-choywmwd	188	10	by	by	IN
cord-104279-choywmwd	188	11	indirect	indirect	JJ
cord-104279-choywmwd	188	12	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	188	13	microscopy	microscopy	NNS
cord-104279-choywmwd	188	14	using	use	VBG
cord-104279-choywmwd	188	15	an	an	DT
cord-104279-choywmwd	188	16	antibody	antibody	NN
cord-104279-choywmwd	188	17	that	that	WDT
cord-104279-choywmwd	188	18	recognizes	recognize	VBZ
cord-104279-choywmwd	188	19	the	the	DT
cord-104279-choywmwd	188	20	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	188	21	domain	domain	NN
cord-104279-choywmwd	188	22	of	of	IN
cord-104279-choywmwd	188	23	DPAP	DPAP	NNP
cord-104279-choywmwd	188	24	B	B	NNP
cord-104279-choywmwd	188	25	(	(	-LRB-
cord-104279-choywmwd	188	26	see	see	VB
cord-104279-choywmwd	188	27	Materials	material	NNS
cord-104279-choywmwd	188	28	and	and	CC
cord-104279-choywmwd	188	29	Methods	Methods	NNPS
cord-104279-choywmwd	188	30	)	)	-RRB-
cord-104279-choywmwd	188	31	.	.	.
cord-104279-choywmwd	189	1	Fig	Fig	NNP
cord-104279-choywmwd	189	2	.	.	NNP
cord-104279-choywmwd	189	3	4	4	CD
cord-104279-choywmwd	189	4	shows	show	VBZ
cord-104279-choywmwd	189	5	the	the	DT
cord-104279-choywmwd	189	6	staining	staining	NN
cord-104279-choywmwd	189	7	pattern	pattern	NN
cord-104279-choywmwd	189	8	of	of	IN
cord-104279-choywmwd	189	9	wild	wild	JJ
cord-104279-choywmwd	189	10	type	type	NN
cord-104279-choywmwd	189	11	DPAP	DPAP	NNP
cord-104279-choywmwd	189	12	B	B	NNP
cord-104279-choywmwd	189	13	(	(	-LRB-
cord-104279-choywmwd	189	14	Fig	Fig	NNP
cord-104279-choywmwd	189	15	.	.	NNP
cord-104279-choywmwd	189	16	4	4	CD
cord-104279-choywmwd	189	17	,	,	,
cord-104279-choywmwd	189	18	A	A	NNP
cord-104279-choywmwd	189	19	-	-	HYPH
cord-104279-choywmwd	189	20	C	C	NNP
cord-104279-choywmwd	189	21	)	)	-RRB-
cord-104279-choywmwd	189	22	and	and	CC
cord-104279-choywmwd	189	23	BB	BB	NNP
cord-104279-choywmwd	189	24	-	-	HYPH
cord-104279-choywmwd	189	25	Inv	Inv	NNP
cord-104279-choywmwd	189	26	(	(	-LRB-
cord-104279-choywmwd	189	27	Fig	Fig	NNP
cord-104279-choywmwd	189	28	.	.	NNP
cord-104279-choywmwd	189	29	4	4	CD
cord-104279-choywmwd	189	30	,	,	,
cord-104279-choywmwd	189	31	G	g	NN
cord-104279-choywmwd	189	32	-	-	HYPH
cord-104279-choywmwd	189	33	I	I	NNP
cord-104279-choywmwd	189	34	)	)	-RRB-
cord-104279-choywmwd	189	35	when	when	WRB
cord-104279-choywmwd	189	36	expressed	express	VBN
cord-104279-choywmwd	189	37	from	from	IN
cord-104279-choywmwd	189	38	the	the	DT
cord-104279-choywmwd	189	39	high	high	JJ
cord-104279-choywmwd	189	40	copy	copy	NN
cord-104279-choywmwd	189	41	number	number	NN
cord-104279-choywmwd	189	42	(	(	-LRB-
cord-104279-choywmwd	189	43	2	2	CD
cord-104279-choywmwd	189	44	#	#	$
cord-104279-choywmwd	189	45	)	)	-RRB-
cord-104279-choywmwd	189	46	plasmids	plasmid	NNS
cord-104279-choywmwd	189	47	,	,	,
cord-104279-choywmwd	189	48	pGP3	pGP3	NNP
cord-104279-choywmwd	189	49	(	(	-LRB-
cord-104279-choywmwd	190	1	Roberts	Roberts	NNP
cord-104279-choywmwd	190	2	et	et	NNP
cord-104279-choywmwd	190	3	al	al	NNP
cord-104279-choywmwd	190	4	.	.	NNP
cord-104279-choywmwd	190	5	,	,	,
cord-104279-choywmwd	190	6	1989	1989	CD
cord-104279-choywmwd	190	7	)	)	-RRB-
cord-104279-choywmwd	190	8	and	and	CC
cord-104279-choywmwd	190	9	pCJR13	pCJR13	NNP
cord-104279-choywmwd	190	10	,	,	,
cord-104279-choywmwd	190	11	respectively	respectively	RB
cord-104279-choywmwd	190	12	.	.	.
cord-104279-choywmwd	191	1	For	for	IN
cord-104279-choywmwd	191	2	these	these	DT
cord-104279-choywmwd	191	3	and	and	CC
cord-104279-choywmwd	191	4	the	the	DT
cord-104279-choywmwd	191	5	other	other	JJ
cord-104279-choywmwd	191	6	2	2	CD
cord-104279-choywmwd	191	7	#	#	NN
cord-104279-choywmwd	191	8	plasmid	plasmid	NN
cord-104279-choywmwd	191	9	constructs	construct	NNS
cord-104279-choywmwd	191	10	used	use	VBN
cord-104279-choywmwd	191	11	in	in	IN
cord-104279-choywmwd	191	12	this	this	DT
cord-104279-choywmwd	191	13	study	study	NN
cord-104279-choywmwd	191	14	,	,	,
cord-104279-choywmwd	191	15	the	the	DT
cord-104279-choywmwd	191	16	proteins	protein	NNS
cord-104279-choywmwd	191	17	were	be	VBD
cord-104279-choywmwd	191	18	overproduced	overproduce	VBN
cord-104279-choywmwd	191	19	10	10	CD
cord-104279-choywmwd	191	20	-	-	HYPH
cord-104279-choywmwd	191	21	20-fold	20-fold	CD
cord-104279-choywmwd	191	22	as	as	IN
cord-104279-choywmwd	191	23	determined	determine	VBN
cord-104279-choywmwd	191	24	by	by	IN
cord-104279-choywmwd	191	25	enzyme	enzyme	NN
cord-104279-choywmwd	191	26	activity	activity	NN
cord-104279-choywmwd	191	27	.	.	.
cord-104279-choywmwd	192	1	Both	both	DT
cord-104279-choywmwd	192	2	DPAP	DPAP	NNP
cord-104279-choywmwd	192	3	B	B	NNP
cord-104279-choywmwd	192	4	and	and	CC
cord-104279-choywmwd	192	5	BB	BB	NNP
cord-104279-choywmwd	192	6	-	-	HYPH
cord-104279-choywmwd	192	7	Inv	Inv	NNP
cord-104279-choywmwd	192	8	were	be	VBD
cord-104279-choywmwd	192	9	localized	localize	VBN
cord-104279-choywmwd	192	10	to	to	IN
cord-104279-choywmwd	192	11	the	the	DT
cord-104279-choywmwd	192	12	vacuolar	vacuolar	JJ
cord-104279-choywmwd	192	13	membrane	membrane	NN
cord-104279-choywmwd	192	14	as	as	IN
cord-104279-choywmwd	192	15	judged	judge	VBN
cord-104279-choywmwd	192	16	by	by	IN
cord-104279-choywmwd	192	17	differential	differential	JJ
cord-104279-choywmwd	192	18	interference	interference	NN
cord-104279-choywmwd	192	19	contrast	contrast	NN
cord-104279-choywmwd	192	20	(	(	-LRB-
cord-104279-choywmwd	192	21	Nomarski	Nomarski	NNP
cord-104279-choywmwd	192	22	)	)	-RRB-
cord-104279-choywmwd	192	23	optics	optic	NNS
cord-104279-choywmwd	192	24	and	and	CC
cord-104279-choywmwd	192	25	co	co	NN
cord-104279-choywmwd	192	26	-	-	NN
cord-104279-choywmwd	192	27	localization	localization	NN
cord-104279-choywmwd	192	28	with	with	IN
cord-104279-choywmwd	192	29	a	a	DT
cord-104279-choywmwd	192	30	marker	marker	NN
cord-104279-choywmwd	192	31	for	for	IN
cord-104279-choywmwd	192	32	the	the	DT
cord-104279-choywmwd	192	33	vacuolar	vacuolar	NNP
cord-104279-choywmwd	192	34	membrane	membrane	NN
cord-104279-choywmwd	192	35	,	,	,
cord-104279-choywmwd	192	36	the	the	DT
cord-104279-choywmwd	192	37	60-kD	60-kd	CD
cord-104279-choywmwd	192	38	subunit	subunit	NN
cord-104279-choywmwd	192	39	of	of	IN
cord-104279-choywmwd	192	40	the	the	DT
cord-104279-choywmwd	192	41	vacuolar	vacuolar	NNP
cord-104279-choywmwd	192	42	H+-ATPase	H+-ATPase	NNP
cord-104279-choywmwd	192	43	(	(	-LRB-
cord-104279-choywmwd	192	44	Vat2p	Vat2p	VBG
cord-104279-choywmwd	192	45	;	;	.
cord-104279-choywmwd	193	1	Yamashiro	Yamashiro	NNP
cord-104279-choywmwd	193	2	et	et	FW
cord-104279-choywmwd	193	3	al	al	NNP
cord-104279-choywmwd	193	4	.	.	NNP
cord-104279-choywmwd	193	5	,	,	,
cord-104279-choywmwd	193	6	1990	1990	CD
cord-104279-choywmwd	193	7	;	;	:
cord-104279-choywmwd	193	8	Table	Table	NNP
cord-104279-choywmwd	193	9	 	 	_SP
cord-104279-choywmwd	193	10	I	I	NNP
cord-104279-choywmwd	193	11	)	)	-RRB-
cord-104279-choywmwd	193	12	.	.	.
cord-104279-choywmwd	194	1	DPAP	DPAP	NNP
cord-104279-choywmwd	194	2	B	B	NNP
cord-104279-choywmwd	194	3	and	and	CC
cord-104279-choywmwd	194	4	BB	BB	NNP
cord-104279-choywmwd	194	5	-	-	HYPH
cord-104279-choywmwd	194	6	Inv	Inv	NNP
cord-104279-choywmwd	194	7	were	be	VBD
cord-104279-choywmwd	194	8	also	also	RB
cord-104279-choywmwd	194	9	localized	localize	VBN
cord-104279-choywmwd	194	10	to	to	IN
cord-104279-choywmwd	194	11	the	the	DT
cord-104279-choywmwd	194	12	vacuolar	vacuolar	JJ
cord-104279-choywmwd	194	13	membrane	membrane	NN
cord-104279-choywmwd	194	14	when	when	WRB
cord-104279-choywmwd	194	15	expressed	express	VBN
cord-104279-choywmwd	194	16	from	from	IN
cord-104279-choywmwd	194	17	a	a	DT
cord-104279-choywmwd	194	18	single	single	JJ
cord-104279-choywmwd	194	19	copy	copy	NN
cord-104279-choywmwd	194	20	(	(	-LRB-
cord-104279-choywmwd	194	21	CEN	cen	NN
cord-104279-choywmwd	194	22	)	)	-RRB-
cord-104279-choywmwd	194	23	plasmid	plasmid	NN
cord-104279-choywmwd	194	24	(	(	-LRB-
cord-104279-choywmwd	194	25	data	datum	NNS
cord-104279-choywmwd	194	26	not	not	RB
cord-104279-choywmwd	194	27	shown	show	VBN
cord-104279-choywmwd	194	28	)	)	-RRB-
cord-104279-choywmwd	194	29	.	.	.
cord-104279-choywmwd	195	1	Aside	aside	RB
cord-104279-choywmwd	195	2	from	from	IN
cord-104279-choywmwd	195	3	the	the	DT
cord-104279-choywmwd	195	4	differences	difference	NNS
cord-104279-choywmwd	195	5	in	in	IN
cord-104279-choywmwd	195	6	signal	signal	JJ
cord-104279-choywmwd	195	7	intensity	intensity	NN
cord-104279-choywmwd	195	8	,	,	,
cord-104279-choywmwd	195	9	no	no	DT
cord-104279-choywmwd	195	10	difference	difference	NN
cord-104279-choywmwd	195	11	in	in	IN
cord-104279-choywmwd	195	12	subcellular	subcellular	JJ
cord-104279-choywmwd	195	13	localization	localization	NN
cord-104279-choywmwd	195	14	was	be	VBD
cord-104279-choywmwd	195	15	observed	observe	VBN
cord-104279-choywmwd	195	16	when	when	WRB
cord-104279-choywmwd	195	17	BB	BB	NNP
cord-104279-choywmwd	195	18	-	-	HYPH
cord-104279-choywmwd	195	19	Inv	Inv	NNP
cord-104279-choywmwd	195	20	was	be	VBD
cord-104279-choywmwd	195	21	expressed	express	VBN
cord-104279-choywmwd	195	22	from	from	IN
cord-104279-choywmwd	195	23	CEN	CEN	NNP
cord-104279-choywmwd	195	24	or	or	CC
cord-104279-choywmwd	195	25	2	2	CD
cord-104279-choywmwd	195	26	#	#	$
cord-104279-choywmwd	195	27	plasmids	plasmid	NNS
cord-104279-choywmwd	195	28	.	.	.
cord-104279-choywmwd	196	1	The	the	DT
cord-104279-choywmwd	196	2	results	result	NNS
cord-104279-choywmwd	196	3	from	from	IN
cord-104279-choywmwd	196	4	the	the	DT
cord-104279-choywmwd	196	5	aFss	aFss	NNP
cord-104279-choywmwd	196	6	-	-	HYPH
cord-104279-choywmwd	196	7	B	b	NN
cord-104279-choywmwd	196	8	and	and	CC
cord-104279-choywmwd	196	9	BB	BB	NNP
cord-104279-choywmwd	196	10	-	-	HYPH
cord-104279-choywmwd	196	11	Inv	inv	JJ
cord-104279-choywmwd	196	12	fusions	fusion	NNS
cord-104279-choywmwd	196	13	indi	indi	NNP
cord-104279-choywmwd	196	14	-	-	HYPH
cord-104279-choywmwd	196	15	cate	cate	NNP
cord-104279-choywmwd	197	1	that	that	IN
cord-104279-choywmwd	197	2	,	,	,
cord-104279-choywmwd	197	3	similar	similar	JJ
cord-104279-choywmwd	197	4	to	to	IN
cord-104279-choywmwd	197	5	ALP	ALP	NNP
cord-104279-choywmwd	197	6	(	(	-LRB-
cord-104279-choywmwd	197	7	Klionsky	Klionsky	NNP
cord-104279-choywmwd	197	8	and	and	CC
cord-104279-choywmwd	197	9	Emr	Emr	NNP
cord-104279-choywmwd	197	10	,	,	,
cord-104279-choywmwd	197	11	1990	1990	CD
cord-104279-choywmwd	197	12	)	)	-RRB-
cord-104279-choywmwd	197	13	,	,	,
cord-104279-choywmwd	197	14	the	the	DT
cord-104279-choywmwd	197	15	lumenal	lumenal	JJ
cord-104279-choywmwd	197	16	domain	domain	NN
cord-104279-choywmwd	197	17	of	of	IN
cord-104279-choywmwd	197	18	DPAP	DPAP	NNP
cord-104279-choywmwd	197	19	B	B	NNP
cord-104279-choywmwd	197	20	is	be	VBZ
cord-104279-choywmwd	197	21	neither	neither	CC
cord-104279-choywmwd	197	22	necessary	necessary	JJ
cord-104279-choywmwd	197	23	nor	nor	CC
cord-104279-choywmwd	197	24	sufficient	sufficient	JJ
cord-104279-choywmwd	197	25	for	for	IN
cord-104279-choywmwd	197	26	vacuolar	vacuolar	NNP
cord-104279-choywmwd	197	27	targeting	targeting	NN
cord-104279-choywmwd	197	28	.	.	.
cord-104279-choywmwd	198	1	The	the	DT
cord-104279-choywmwd	198	2	role	role	NN
cord-104279-choywmwd	198	3	of	of	IN
cord-104279-choywmwd	198	4	the	the	DT
cord-104279-choywmwd	198	5	transmembrane	transmembrane	NN
cord-104279-choywmwd	198	6	domain	domain	NN
cord-104279-choywmwd	198	7	in	in	IN
cord-104279-choywmwd	198	8	the	the	DT
cord-104279-choywmwd	198	9	vacuolar	vacuolar	JJ
cord-104279-choywmwd	198	10	targeting	targeting	NN
cord-104279-choywmwd	198	11	of	of	IN
cord-104279-choywmwd	198	12	DPAP	DPAP	NNP
cord-104279-choywmwd	198	13	B	B	NNP
cord-104279-choywmwd	198	14	was	be	VBD
cord-104279-choywmwd	198	15	tested	test	VBN
cord-104279-choywmwd	198	16	by	by	IN
cord-104279-choywmwd	198	17	constructing	construct	VBG
cord-104279-choywmwd	198	18	the	the	DT
cord-104279-choywmwd	198	19	fusion	fusion	NN
cord-104279-choywmwd	198	20	protein	protein	NN
cord-104279-choywmwd	198	21	B	B	NNP
cord-104279-choywmwd	198	22	-	-	HYPH
cord-104279-choywmwd	198	23	A	A	NNP
cord-104279-choywmwd	198	24	-	-	HYPH
cord-104279-choywmwd	198	25	B	b	NN
cord-104279-choywmwd	198	26	(	(	-LRB-
cord-104279-choywmwd	198	27	Fig	fig	NN
cord-104279-choywmwd	198	28	.	.	.
cord-104279-choywmwd	198	29	1	1	CD
cord-104279-choywmwd	198	30	B	b	NN
cord-104279-choywmwd	198	31	)	)	-RRB-
cord-104279-choywmwd	198	32	,	,	,
cord-104279-choywmwd	198	33	in	in	IN
cord-104279-choywmwd	198	34	which	which	WDT
cord-104279-choywmwd	198	35	the	the	DT
cord-104279-choywmwd	198	36	membrane	membrane	NN
cord-104279-choywmwd	198	37	anchor	anchor	NN
cord-104279-choywmwd	198	38	of	of	IN
cord-104279-choywmwd	198	39	DPAP	DPAP	NNP
cord-104279-choywmwd	198	40	B	B	NNP
cord-104279-choywmwd	198	41	was	be	VBD
cord-104279-choywmwd	198	42	replaced	replace	VBN
cord-104279-choywmwd	198	43	by	by	IN
cord-104279-choywmwd	198	44	that	that	DT
cord-104279-choywmwd	198	45	of	of	IN
cord-104279-choywmwd	198	46	the	the	DT
cord-104279-choywmwd	198	47	non	non	JJ
cord-104279-choywmwd	198	48	-	-	JJ
cord-104279-choywmwd	198	49	vacuolar	vacuolar	JJ
cord-104279-choywmwd	198	50	membrane	membrane	NN
cord-104279-choywmwd	198	51	protein	protein	NN
cord-104279-choywmwd	198	52	,	,	,
cord-104279-choywmwd	198	53	DPAP	DPAP	NNP
cord-104279-choywmwd	198	54	A.	a.	NN
cord-104279-choywmwd	199	1	The	the	DT
cord-104279-choywmwd	199	2	role	role	NN
cord-104279-choywmwd	199	3	of	of	IN
cord-104279-choywmwd	199	4	the	the	DT
cord-104279-choywmwd	199	5	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	199	6	domain	domain	NN
cord-104279-choywmwd	199	7	of	of	IN
cord-104279-choywmwd	199	8	DPAP	DPAP	NNP
cord-104279-choywmwd	199	9	B	B	NNP
cord-104279-choywmwd	199	10	was	be	VBD
cord-104279-choywmwd	199	11	tested	test	VBN
cord-104279-choywmwd	199	12	by	by	IN
cord-104279-choywmwd	199	13	constructing	construct	VBG
cord-104279-choywmwd	199	14	in	in	IN
cord-104279-choywmwd	199	15	-	-	HYPH
cord-104279-choywmwd	199	16	frame	frame	NN
cord-104279-choywmwd	199	17	deletions	deletion	NNS
cord-104279-choywmwd	199	18	in	in	IN
cord-104279-choywmwd	199	19	this	this	DT
cord-104279-choywmwd	199	20	domain	domain	NN
cord-104279-choywmwd	199	21	using	use	VBG
cord-104279-choywmwd	199	22	oligonucleotide	oligonucleotide	RB
cord-104279-choywmwd	199	23	-	-	HYPH
cord-104279-choywmwd	199	24	directed	direct	VBN
cord-104279-choywmwd	199	25	mutagenesis	mutagenesis	NN
cord-104279-choywmwd	199	26	of	of	IN
cord-104279-choywmwd	199	27	the	the	DT
cord-104279-choywmwd	199	28	DAP2	DAP2	NNP
cord-104279-choywmwd	199	29	gene	gene	NN
cord-104279-choywmwd	199	30	.	.	.
cord-104279-choywmwd	200	1	Two	two	CD
cord-104279-choywmwd	200	2	deletion	deletion	NN
cord-104279-choywmwd	200	3	variants	variant	NNS
cord-104279-choywmwd	200	4	of	of	IN
cord-104279-choywmwd	200	5	DPAP	DPAP	NNP
cord-104279-choywmwd	200	6	B	B	NNP
cord-104279-choywmwd	200	7	were	be	VBD
cord-104279-choywmwd	200	8	constructed	construct	VBN
cord-104279-choywmwd	200	9	,	,	,
cord-104279-choywmwd	200	10	A20-BB	A20-BB	NNP
cord-104279-choywmwd	200	11	and	and	CC
cord-104279-choywmwd	200	12	A27-BB	A27-BB	NNP
cord-104279-choywmwd	200	13	,	,	,
cord-104279-choywmwd	200	14	in	in	IN
cord-104279-choywmwd	200	15	which	which	WDT
cord-104279-choywmwd	200	16	20	20	CD
cord-104279-choywmwd	200	17	and	and	CC
cord-104279-choywmwd	200	18	27	27	CD
cord-104279-choywmwd	200	19	amino	amino	JJ
cord-104279-choywmwd	200	20	acids	acid	NNS
cord-104279-choywmwd	200	21	,	,	,
cord-104279-choywmwd	200	22	respectively	respectively	RB
cord-104279-choywmwd	200	23	,	,	,
cord-104279-choywmwd	200	24	were	be	VBD
cord-104279-choywmwd	200	25	removed	remove	VBN
cord-104279-choywmwd	200	26	from	from	IN
cord-104279-choywmwd	200	27	the	the	DT
cord-104279-choywmwd	200	28	29	29	CD
cord-104279-choywmwd	200	29	residue	residue	NN
cord-104279-choywmwd	200	30	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	200	31	domain	domain	NN
cord-104279-choywmwd	200	32	(	(	-LRB-
cord-104279-choywmwd	200	33	Fig	fig	NN
cord-104279-choywmwd	200	34	.	.	.
cord-104279-choywmwd	200	35	1	1	CD
cord-104279-choywmwd	200	36	B	b	NN
cord-104279-choywmwd	200	37	;	;	:
cord-104279-choywmwd	200	38	see	see	VB
cord-104279-choywmwd	200	39	Materials	material	NNS
cord-104279-choywmwd	200	40	and	and	CC
cord-104279-choywmwd	200	41	Methods	Methods	NNPS
cord-104279-choywmwd	200	42	)	)	-RRB-
cord-104279-choywmwd	200	43	.	.	.
cord-104279-choywmwd	201	1	Immunoprecipitations	immunoprecipitation	NNS
cord-104279-choywmwd	201	2	of	of	IN
cord-104279-choywmwd	201	3	B	B	NNP
cord-104279-choywmwd	201	4	-	-	HYPH
cord-104279-choywmwd	201	5	A	A	NNP
cord-104279-choywmwd	201	6	-	-	HYPH
cord-104279-choywmwd	201	7	B	b	NN
cord-104279-choywmwd	201	8	,	,	,
cord-104279-choywmwd	201	9	A20-BB	A20-BB	NNP
cord-104279-choywmwd	201	10	,	,	,
cord-104279-choywmwd	201	11	and	and	CC
cord-104279-choywmwd	201	12	A27-BB	a27-bb	NN
cord-104279-choywmwd	201	13	from	from	IN
cord-104279-choywmwd	201	14	35S	35S	NNP
cord-104279-choywmwd	201	15	-	-	HYPH
cord-104279-choywmwd	201	16	labeled	label	VBN
cord-104279-choywmwd	201	17	cells	cell	NNS
cord-104279-choywmwd	201	18	,	,	,
cord-104279-choywmwd	201	19	followed	follow	VBN
cord-104279-choywmwd	201	20	by	by	IN
cord-104279-choywmwd	201	21	SDS	SDS	NNP
cord-104279-choywmwd	201	22	-	-	HYPH
cord-104279-choywmwd	201	23	PAGE	PAGE	NNP
cord-104279-choywmwd	201	24	and	and	CC
cord-104279-choywmwd	201	25	fluorography	fluorography	NNP
cord-104279-choywmwd	201	26	,	,	,
cord-104279-choywmwd	201	27	showed	show	VBD
cord-104279-choywmwd	201	28	that	that	IN
cord-104279-choywmwd	201	29	the	the	DT
cord-104279-choywmwd	201	30	mutant	mutant	JJ
cord-104279-choywmwd	201	31	proteins	protein	NNS
cord-104279-choywmwd	201	32	were	be	VBD
cord-104279-choywmwd	201	33	glycosylated	glycosylate	VBN
cord-104279-choywmwd	201	34	,	,	,
cord-104279-choywmwd	201	35	and	and	CC
cord-104279-choywmwd	201	36	that	that	IN
cord-104279-choywmwd	201	37	the	the	DT
cord-104279-choywmwd	201	38	deglycosylated	deglycosylated	JJ
cord-104279-choywmwd	201	39	proteins	protein	NNS
cord-104279-choywmwd	201	40	were	be	VBD
cord-104279-choywmwd	201	41	of	of	IN
cord-104279-choywmwd	201	42	the	the	DT
cord-104279-choywmwd	201	43	predicted	predict	VBN
cord-104279-choywmwd	201	44	size	size	NN
cord-104279-choywmwd	201	45	(	(	-LRB-
cord-104279-choywmwd	201	46	Fig	fig	NN
cord-104279-choywmwd	201	47	.	.	.
cord-104279-choywmwd	201	48	  	  	_SP
cord-104279-choywmwd	202	1	3	3	LS
cord-104279-choywmwd	202	2	,	,	,
cord-104279-choywmwd	202	3	lanes	lane	VBZ
cord-104279-choywmwd	202	4	3	3	CD
cord-104279-choywmwd	202	5	-	-	SYM
cord-104279-choywmwd	202	6	8	8	CD
cord-104279-choywmwd	202	7	)	)	-RRB-
cord-104279-choywmwd	202	8	.	.	.
cord-104279-choywmwd	203	1	As	as	IN
cord-104279-choywmwd	203	2	with	with	IN
cord-104279-choywmwd	203	3	BB	BB	NNP
cord-104279-choywmwd	203	4	-	-	HYPH
cord-104279-choywmwd	203	5	Inv	Inv	NNP
cord-104279-choywmwd	203	6	,	,	,
cord-104279-choywmwd	203	7	these	these	DT
cord-104279-choywmwd	203	8	proteins	protein	NNS
cord-104279-choywmwd	203	9	behaved	behave	VBD
cord-104279-choywmwd	203	10	as	as	IN
cord-104279-choywmwd	203	11	integral	integral	JJ
cord-104279-choywmwd	203	12	membrane	membrane	NN
cord-104279-choywmwd	203	13	proteins	protein	NNS
cord-104279-choywmwd	203	14	in	in	IN
cord-104279-choywmwd	203	15	high	high	JJ
cord-104279-choywmwd	203	16	pH	ph	NN
cord-104279-choywmwd	203	17	carbonate	carbonate	NN
cord-104279-choywmwd	203	18	fractionation	fractionation	NN
cord-104279-choywmwd	203	19	experiments	experiment	NNS
cord-104279-choywmwd	203	20	(	(	-LRB-
cord-104279-choywmwd	203	21	data	datum	NNS
cord-104279-choywmwd	203	22	not	not	RB
cord-104279-choywmwd	203	23	shown	show	VBN
cord-104279-choywmwd	203	24	)	)	-RRB-
cord-104279-choywmwd	203	25	.	.	.
cord-104279-choywmwd	204	1	DPAP	DPAP	NNP
cord-104279-choywmwd	204	2	activity	activity	NN
cord-104279-choywmwd	204	3	assays	assay	NNS
cord-104279-choywmwd	204	4	of	of	IN
cord-104279-choywmwd	204	5	cells	cell	NNS
cord-104279-choywmwd	204	6	expressing	express	VBG
cord-104279-choywmwd	204	7	B	b	NN
cord-104279-choywmwd	204	8	-	-	HYPH
cord-104279-choywmwd	204	9	A	a	NN
cord-104279-choywmwd	204	10	-	-	HYPH
cord-104279-choywmwd	204	11	B	b	NN
cord-104279-choywmwd	204	12	,	,	,
cord-104279-choywmwd	204	13	A20-BB	A20-BB	NNP
cord-104279-choywmwd	204	14	,	,	,
cord-104279-choywmwd	204	15	and	and	CC
cord-104279-choywmwd	204	16	A27-BB	A27-BB	NNP
cord-104279-choywmwd	204	17	showed	show	VBD
cord-104279-choywmwd	204	18	that	that	IN
cord-104279-choywmwd	204	19	the	the	DT
cord-104279-choywmwd	204	20	proteins	protein	NNS
cord-104279-choywmwd	204	21	were	be	VBD
cord-104279-choywmwd	204	22	fully	fully	RB
cord-104279-choywmwd	204	23	enzymatically	enzymatically	RB
cord-104279-choywmwd	204	24	active	active	JJ
cord-104279-choywmwd	204	25	,	,	,
cord-104279-choywmwd	204	26	and	and	CC
cord-104279-choywmwd	204	27	that	that	IN
cord-104279-choywmwd	204	28	88	88	CD
cord-104279-choywmwd	204	29	-	-	SYM
cord-104279-choywmwd	204	30	98	98	CD
cord-104279-choywmwd	204	31	%	%	NN
cord-104279-choywmwd	204	32	of	of	IN
cord-104279-choywmwd	204	33	the	the	DT
cord-104279-choywmwd	204	34	total	total	JJ
cord-104279-choywmwd	204	35	activity	activity	NN
cord-104279-choywmwd	204	36	was	be	VBD
cord-104279-choywmwd	204	37	intracellular	intracellular	JJ
cord-104279-choywmwd	204	38	.	.	.
cord-104279-choywmwd	205	1	Indirect	indirect	JJ
cord-104279-choywmwd	205	2	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	205	3	microscopy	microscopy	NN
cord-104279-choywmwd	205	4	of	of	IN
cord-104279-choywmwd	205	5	ceils	ceil	NNS
cord-104279-choywmwd	205	6	expressing	express	VBG
cord-104279-choywmwd	205	7	B	B	NNP
cord-104279-choywmwd	205	8	-	-	HYPH
cord-104279-choywmwd	205	9	A	a	NN
cord-104279-choywmwd	205	10	-	-	HYPH
cord-104279-choywmwd	205	11	B	b	NN
cord-104279-choywmwd	205	12	,	,	,
cord-104279-choywmwd	205	13	A20-BB	A20-BB	NNP
cord-104279-choywmwd	205	14	,	,	,
cord-104279-choywmwd	205	15	and	and	CC
cord-104279-choywmwd	205	16	A27-BB	a27-bb	NN
cord-104279-choywmwd	205	17	from	from	IN
cord-104279-choywmwd	205	18	either	either	DT
cord-104279-choywmwd	205	19	2	2	CD
cord-104279-choywmwd	205	20	#	#	NN
cord-104279-choywmwd	205	21	plasmids	plasmid	NNS
cord-104279-choywmwd	205	22	(	(	-LRB-
cord-104279-choywmwd	205	23	Fig	Fig	NNP
cord-104279-choywmwd	205	24	.	.	NNP
cord-104279-choywmwd	205	25	5	5	CD
cord-104279-choywmwd	205	26	,	,	,
cord-104279-choywmwd	205	27	C	C	NNP
cord-104279-choywmwd	205	28	-	-	HYPH
cord-104279-choywmwd	205	29	H	H	NNP
cord-104279-choywmwd	205	30	)	)	-RRB-
cord-104279-choywmwd	205	31	or	or	CC
cord-104279-choywmwd	205	32	CEN	CEN	NNP
cord-104279-choywmwd	205	33	plasmids	plasmid	NNS
cord-104279-choywmwd	205	34	,	,	,
cord-104279-choywmwd	205	35	using	use	VBG
cord-104279-choywmwd	205	36	an	an	DT
cord-104279-choywmwd	205	37	antibody	antibody	NN
cord-104279-choywmwd	205	38	that	that	WDT
cord-104279-choywmwd	205	39	recognizes	recognize	VBZ
cord-104279-choywmwd	205	40	the	the	DT
cord-104279-choywmwd	205	41	COOH	COOH	NNP
cord-104279-choywmwd	205	42	-	-	HYPH
cord-104279-choywmwd	205	43	terminal	terminal	JJ
cord-104279-choywmwd	205	44	half	half	NN
cord-104279-choywmwd	205	45	of	of	IN
cord-104279-choywmwd	205	46	DPAP	DPAP	NNP
cord-104279-choywmwd	205	47	B	B	NNP
cord-104279-choywmwd	205	48	,	,	,
cord-104279-choywmwd	205	49	showed	show	VBD
cord-104279-choywmwd	205	50	that	that	IN
cord-104279-choywmwd	205	51	each	each	DT
cord-104279-choywmwd	205	52	of	of	IN
cord-104279-choywmwd	205	53	the	the	DT
cord-104279-choywmwd	205	54	mutant	mutant	JJ
cord-104279-choywmwd	205	55	proteins	protein	NNS
cord-104279-choywmwd	205	56	was	be	VBD
cord-104279-choywmwd	205	57	predominantly	predominantly	RB
cord-104279-choywmwd	205	58	localized	localize	VBN
cord-104279-choywmwd	205	59	to	to	IN
cord-104279-choywmwd	205	60	the	the	DT
cord-104279-choywmwd	205	61	vacuolar	vacuolar	JJ
cord-104279-choywmwd	205	62	membrane	membrane	NN
cord-104279-choywmwd	205	63	as	as	IN
cord-104279-choywmwd	205	64	determined	determine	VBN
cord-104279-choywmwd	205	65	by	by	IN
cord-104279-choywmwd	205	66	Nomarski	Nomarski	NNP
cord-104279-choywmwd	205	67	optics	optic	NNS
cord-104279-choywmwd	205	68	(	(	-LRB-
cord-104279-choywmwd	206	1	Fig	Fig	NNP
cord-104279-choywmwd	206	2	.	.	NNP
cord-104279-choywmwd	206	3	5	5	CD
cord-104279-choywmwd	206	4	)	)	-RRB-
cord-104279-choywmwd	206	5	and	and	CC
cord-104279-choywmwd	206	6	co	co	NN
cord-104279-choywmwd	206	7	-	-	NN
cord-104279-choywmwd	206	8	localization	localization	NN
cord-104279-choywmwd	206	9	with	with	IN
cord-104279-choywmwd	206	10	Vat2p	Vat2p	NNP
cord-104279-choywmwd	206	11	(	(	-LRB-
cord-104279-choywmwd	206	12	data	datum	NNS
cord-104279-choywmwd	206	13	not	not	RB
cord-104279-choywmwd	206	14	shown	show	VBN
cord-104279-choywmwd	206	15	)	)	-RRB-
cord-104279-choywmwd	206	16	.	.	.
cord-104279-choywmwd	207	1	No	no	DT
cord-104279-choywmwd	207	2	staining	staining	NN
cord-104279-choywmwd	207	3	of	of	IN
cord-104279-choywmwd	207	4	the	the	DT
cord-104279-choywmwd	207	5	plasma	plasma	NN
cord-104279-choywmwd	207	6	membrane	membrane	NN
cord-104279-choywmwd	207	7	was	be	VBD
cord-104279-choywmwd	207	8	observed	observe	VBN
cord-104279-choywmwd	207	9	for	for	IN
cord-104279-choywmwd	207	10	any	any	DT
cord-104279-choywmwd	207	11	of	of	IN
cord-104279-choywmwd	207	12	these	these	DT
cord-104279-choywmwd	207	13	proteins	protein	NNS
cord-104279-choywmwd	207	14	.	.	.
cord-104279-choywmwd	208	1	Thus	thus	RB
cord-104279-choywmwd	208	2	,	,	,
cord-104279-choywmwd	208	3	the	the	DT
cord-104279-choywmwd	208	4	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	208	5	and	and	CC
cord-104279-choywmwd	208	6	transmembrane	transmembrane	JJ
cord-104279-choywmwd	208	7	domains	domain	NNS
cord-104279-choywmwd	208	8	of	of	IN
cord-104279-choywmwd	208	9	DPAP	DPAP	NNP
cord-104279-choywmwd	208	10	B	B	NNP
cord-104279-choywmwd	208	11	are	be	VBP
cord-104279-choywmwd	208	12	not	not	RB
cord-104279-choywmwd	208	13	necessary	necessary	JJ
cord-104279-choywmwd	208	14	for	for	IN
cord-104279-choywmwd	208	15	targeting	target	VBG
cord-104279-choywmwd	208	16	to	to	IN
cord-104279-choywmwd	208	17	the	the	DT
cord-104279-choywmwd	208	18	vacuolar	vacuolar	JJ
cord-104279-choywmwd	208	19	membrane	membrane	NN
cord-104279-choywmwd	208	20	.	.	.
cord-104279-choywmwd	209	1	A	a	DT
cord-104279-choywmwd	209	2	significant	significant	JJ
cord-104279-choywmwd	209	3	fraction	fraction	NN
cord-104279-choywmwd	209	4	of	of	IN
cord-104279-choywmwd	209	5	cells	cell	NNS
cord-104279-choywmwd	209	6	expressing	express	VBG
cord-104279-choywmwd	209	7	A20-BB	a20-bb	CD
cord-104279-choywmwd	209	8	and	and	CC
cord-104279-choywmwd	209	9	A27-BB	A27-BB	NNP
cord-104279-choywmwd	209	10	also	also	RB
cord-104279-choywmwd	209	11	showed	show	VBD
cord-104279-choywmwd	209	12	some	some	DT
cord-104279-choywmwd	209	13	ER	er	NN
cord-104279-choywmwd	209	14	localization	localization	NN
cord-104279-choywmwd	209	15	,	,	,
cord-104279-choywmwd	209	16	as	as	IN
cord-104279-choywmwd	209	17	judged	judge	VBN
cord-104279-choywmwd	209	18	by	by	IN
cord-104279-choywmwd	209	19	staining	stain	VBG
cord-104279-choywmwd	209	20	of	of	IN
cord-104279-choywmwd	209	21	the	the	DT
cord-104279-choywmwd	209	22	perinuclear	perinuclear	NNP
cord-104279-choywmwd	209	23	space	space	NN
cord-104279-choywmwd	209	24	and	and	CC
cord-104279-choywmwd	209	25	long	long	JJ
cord-104279-choywmwd	209	26	cisternal	cisternal	JJ
cord-104279-choywmwd	209	27	compartments	compartment	NNS
cord-104279-choywmwd	210	1	(	(	-LRB-
cord-104279-choywmwd	210	2	Table	table	NN
cord-104279-choywmwd	210	3	I	-PRON-	PRP
cord-104279-choywmwd	210	4	;	;	.
cord-104279-choywmwd	211	1	Rose	Rose	NNP
cord-104279-choywmwd	211	2	et	et	FW
cord-104279-choywmwd	211	3	al	al	NNP
cord-104279-choywmwd	211	4	.	.	NNP
cord-104279-choywmwd	211	5	,	,	,
cord-104279-choywmwd	211	6	1989	1989	CD
cord-104279-choywmwd	211	7	)	)	-RRB-
cord-104279-choywmwd	211	8	,	,	.
cord-104279-choywmwd	212	1	whereas	whereas	IN
cord-104279-choywmwd	212	2	B	b	NN
cord-104279-choywmwd	212	3	-	-	HYPH
cord-104279-choywmwd	212	4	A	A	NNP
cord-104279-choywmwd	212	5	-	-	HYPH
cord-104279-choywmwd	212	6	B	b	NN
cord-104279-choywmwd	212	7	showed	show	VBD
cord-104279-choywmwd	212	8	only	only	RB
cord-104279-choywmwd	212	9	vacuolar	vacuolar	JJ
cord-104279-choywmwd	212	10	labeling	labeling	NN
cord-104279-choywmwd	212	11	.	.	.
cord-104279-choywmwd	213	1	The	the	DT
cord-104279-choywmwd	213	2	z~20-BB	z~20-BB	NNP
cord-104279-choywmwd	213	3	and	and	CC
cord-104279-choywmwd	213	4	~27-BB	~27-bb	DT
cord-104279-choywmwd	213	5	cells	cell	NNS
cord-104279-choywmwd	213	6	in	in	IN
cord-104279-choywmwd	213	7	Fig	Fig	NNP
cord-104279-choywmwd	213	8	.	.	.
cord-104279-choywmwd	214	1	5	5	CD
cord-104279-choywmwd	214	2	show	show	VBP
cord-104279-choywmwd	214	3	predominantly	predominantly	RB
cord-104279-choywmwd	214	4	vacuolar	vacuolar	JJ
cord-104279-choywmwd	214	5	staining	staining	NN
cord-104279-choywmwd	214	6	.	.	.
cord-104279-choywmwd	215	1	In	in	IN
cord-104279-choywmwd	215	2	many	many	JJ
cord-104279-choywmwd	215	3	cases	case	NNS
cord-104279-choywmwd	215	4	,	,	,
cord-104279-choywmwd	215	5	the	the	DT
cord-104279-choywmwd	215	6	plane	plane	NN
cord-104279-choywmwd	215	7	of	of	IN
cord-104279-choywmwd	215	8	focus	focus	NN
cord-104279-choywmwd	215	9	had	have	VBD
cord-104279-choywmwd	215	10	to	to	TO
cord-104279-choywmwd	215	11	be	be	VB
cord-104279-choywmwd	215	12	adjusted	adjust	VBN
cord-104279-choywmwd	215	13	to	to	TO
cord-104279-choywmwd	215	14	observe	observe	VB
cord-104279-choywmwd	215	15	staining	staining	NN
cord-104279-choywmwd	215	16	of	of	IN
cord-104279-choywmwd	215	17	both	both	DT
cord-104279-choywmwd	215	18	vacuolar	vacuolar	NN
cord-104279-choywmwd	215	19	and	and	CC
cord-104279-choywmwd	215	20	ER	ER	NNP
cord-104279-choywmwd	215	21	structures	structure	NNS
cord-104279-choywmwd	215	22	within	within	IN
cord-104279-choywmwd	215	23	a	a	DT
cord-104279-choywmwd	215	24	given	give	VBN
cord-104279-choywmwd	215	25	cell	cell	NN
cord-104279-choywmwd	215	26	.	.	.
cord-104279-choywmwd	216	1	The	the	DT
cord-104279-choywmwd	216	2	increased	increase	VBN
cord-104279-choywmwd	216	3	residence	residence	NN
cord-104279-choywmwd	216	4	time	time	NN
cord-104279-choywmwd	216	5	of	of	IN
cord-104279-choywmwd	216	6	A20-BB	a20-bb	NN
cord-104279-choywmwd	216	7	and	and	CC
cord-104279-choywmwd	216	8	A27-BB	a27-bb	NN
cord-104279-choywmwd	216	9	in	in	IN
cord-104279-choywmwd	216	10	the	the	DT
cord-104279-choywmwd	216	11	ER	ER	NNP
cord-104279-choywmwd	216	12	was	be	VBD
cord-104279-choywmwd	216	13	corroborated	corroborate	VBN
cord-104279-choywmwd	216	14	by	by	IN
cord-104279-choywmwd	216	15	immunoblotting	immunoblotte	VBG
cord-104279-choywmwd	216	16	analysis	analysis	NN
cord-104279-choywmwd	216	17	,	,	,
cord-104279-choywmwd	216	18	which	which	WDT
cord-104279-choywmwd	216	19	showed	show	VBD
cord-104279-choywmwd	216	20	that	that	IN
cord-104279-choywmwd	216	21	a	a	DT
cord-104279-choywmwd	216	22	small	small	JJ
cord-104279-choywmwd	216	23	amount	amount	NN
cord-104279-choywmwd	216	24	of	of	IN
cord-104279-choywmwd	216	25	the	the	DT
cord-104279-choywmwd	216	26	ER	ER	NNP
cord-104279-choywmwd	216	27	forms	form	NNS
cord-104279-choywmwd	216	28	of	of	IN
cord-104279-choywmwd	216	29	A20-BB	a20-bb	CD
cord-104279-choywmwd	216	30	and	and	CC
cord-104279-choywmwd	216	31	A27-BB	A27-BB	NNP
cord-104279-choywmwd	216	32	were	be	VBD
cord-104279-choywmwd	216	33	seen	see	VBN
cord-104279-choywmwd	216	34	in	in	IN
cord-104279-choywmwd	216	35	the	the	DT
cord-104279-choywmwd	216	36	steady	steady	JJ
cord-104279-choywmwd	216	37	state	state	NN
cord-104279-choywmwd	216	38	,	,	,
cord-104279-choywmwd	216	39	along	along	IN
cord-104279-choywmwd	216	40	with	with	IN
cord-104279-choywmwd	216	41	the	the	DT
cord-104279-choywmwd	216	42	Golgi	Golgi	NNP
cord-104279-choywmwd	216	43	-	-	HYPH
cord-104279-choywmwd	216	44	modified	modify	VBN
cord-104279-choywmwd	216	45	forms	form	NNS
cord-104279-choywmwd	216	46	,	,	,
cord-104279-choywmwd	216	47	whereas	whereas	IN
cord-104279-choywmwd	216	48	only	only	RB
cord-104279-choywmwd	216	49	the	the	DT
cord-104279-choywmwd	216	50	Golgi	golgi	NN
cord-104279-choywmwd	216	51	-	-	HYPH
cord-104279-choywmwd	216	52	modified	modify	VBN
cord-104279-choywmwd	216	53	form	form	NN
cord-104279-choywmwd	216	54	was	be	VBD
cord-104279-choywmwd	216	55	seen	see	VBN
cord-104279-choywmwd	216	56	for	for	IN
cord-104279-choywmwd	216	57	wild	wild	JJ
cord-104279-choywmwd	216	58	type	type	NN
cord-104279-choywmwd	216	59	DPAP	DPAP	NNP
cord-104279-choywmwd	216	60	B	B	NNP
cord-104279-choywmwd	216	61	(	(	-LRB-
cord-104279-choywmwd	216	62	data	datum	NNS
cord-104279-choywmwd	216	63	not	not	RB
cord-104279-choywmwd	216	64	shown	show	VBN
cord-104279-choywmwd	216	65	)	)	-RRB-
cord-104279-choywmwd	216	66	.	.	.
cord-104279-choywmwd	217	1	The	the	DT
cord-104279-choywmwd	217	2	increased	increase	VBN
cord-104279-choywmwd	217	3	ER	er	NN
cord-104279-choywmwd	217	4	retention	retention	NN
cord-104279-choywmwd	217	5	of	of	IN
cord-104279-choywmwd	217	6	A20-BB	a20-bb	NN
cord-104279-choywmwd	217	7	and	and	CC
cord-104279-choywmwd	217	8	A27-BB	A27-BB	NNP
cord-104279-choywmwd	217	9	could	could	MD
cord-104279-choywmwd	217	10	be	be	VB
cord-104279-choywmwd	217	11	due	due	JJ
cord-104279-choywmwd	217	12	to	to	IN
cord-104279-choywmwd	217	13	an	an	DT
cord-104279-choywmwd	217	14	impaired	impaired	JJ
cord-104279-choywmwd	217	15	ability	ability	NN
cord-104279-choywmwd	217	16	to	to	TO
cord-104279-choywmwd	217	17	achieve	achieve	VB
cord-104279-choywmwd	217	18	a	a	DT
cord-104279-choywmwd	217	19	native	native	JJ
cord-104279-choywmwd	217	20	conformation	conformation	NN
cord-104279-choywmwd	217	21	competent	competent	JJ
cord-104279-choywmwd	217	22	for	for	IN
cord-104279-choywmwd	217	23	exiting	exit	VBG
cord-104279-choywmwd	217	24	the	the	DT
cord-104279-choywmwd	217	25	ER	ER	NNP
cord-104279-choywmwd	217	26	,	,	,
cord-104279-choywmwd	217	27	as	as	IN
cord-104279-choywmwd	217	28	has	have	VBZ
cord-104279-choywmwd	217	29	been	be	VBN
cord-104279-choywmwd	217	30	observed	observe	VBN
cord-104279-choywmwd	217	31	with	with	IN
cord-104279-choywmwd	217	32	mutant	mutant	JJ
cord-104279-choywmwd	217	33	membrane	membrane	NN
cord-104279-choywmwd	217	34	proteins	protein	NNS
cord-104279-choywmwd	217	35	in	in	IN
cord-104279-choywmwd	217	36	mammalian	mammalian	JJ
cord-104279-choywmwd	217	37	cells	cell	NNS
cord-104279-choywmwd	217	38	(	(	-LRB-
cord-104279-choywmwd	217	39	Gething	Gething	NNP
cord-104279-choywmwd	217	40	et	et	FW
cord-104279-choywmwd	217	41	al	al	NNP
cord-104279-choywmwd	217	42	.	.	NNP
cord-104279-choywmwd	217	43	,	,	,
cord-104279-choywmwd	217	44	1986	1986	CD
cord-104279-choywmwd	217	45	;	;	:
cord-104279-choywmwd	217	46	Doms	Doms	NNP
cord-104279-choywmwd	217	47	et	et	FW
cord-104279-choywmwd	217	48	al	al	NNP
cord-104279-choywmwd	217	49	.	.	NNP
cord-104279-choywmwd	217	50	,	,	,
cord-104279-choywmwd	217	51	1988	1988	CD
cord-104279-choywmwd	217	52	)	)	-RRB-
cord-104279-choywmwd	217	53	.	.	.
cord-104279-choywmwd	218	1	Upon	upon	IN
cord-104279-choywmwd	218	2	exiting	exit	VBG
cord-104279-choywmwd	218	3	the	the	DT
cord-104279-choywmwd	218	4	ER	ER	NNP
cord-104279-choywmwd	218	5	,	,	,
cord-104279-choywmwd	218	6	however	however	RB
cord-104279-choywmwd	218	7	,	,	,
cord-104279-choywmwd	218	8	A20-BB	a20-bb	NN
cord-104279-choywmwd	218	9	and	and	CC
cord-104279-choywmwd	218	10	A27-BB	A27-BB	NNP
cord-104279-choywmwd	218	11	were	be	VBD
cord-104279-choywmwd	218	12	transported	transport	VBN
cord-104279-choywmwd	218	13	through	through	IN
cord-104279-choywmwd	218	14	the	the	DT
cord-104279-choywmwd	218	15	Golgi	Golgi	NNP
cord-104279-choywmwd	218	16	complex	complex	NN
cord-104279-choywmwd	218	17	to	to	IN
cord-104279-choywmwd	218	18	the	the	DT
cord-104279-choywmwd	218	19	vacuole	vacuole	NN
cord-104279-choywmwd	218	20	,	,	,
cord-104279-choywmwd	218	21	indicating	indicate	VBG
cord-104279-choywmwd	218	22	that	that	IN
cord-104279-choywmwd	218	23	the	the	DT
cord-104279-choywmwd	218	24	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	218	25	domain	domain	NN
cord-104279-choywmwd	218	26	of	of	IN
cord-104279-choywmwd	218	27	DPAP	DPAP	NNP
cord-104279-choywmwd	218	28	B	B	NNP
cord-104279-choywmwd	218	29	is	be	VBZ
cord-104279-choywmwd	218	30	not	not	RB
cord-104279-choywmwd	218	31	necessary	necessary	JJ
cord-104279-choywmwd	218	32	for	for	IN
cord-104279-choywmwd	218	33	vacuolar	vacuolar	JJ
cord-104279-choywmwd	218	34	localization	localization	NN
cord-104279-choywmwd	218	35	.	.	.
cord-104279-choywmwd	219	1	These	these	DT
cord-104279-choywmwd	219	2	results	result	NNS
cord-104279-choywmwd	219	3	,	,	,
cord-104279-choywmwd	219	4	combined	combine	VBN
cord-104279-choywmwd	219	5	with	with	IN
cord-104279-choywmwd	219	6	those	those	DT
cord-104279-choywmwd	219	7	from	from	IN
cord-104279-choywmwd	219	8	the	the	DT
cord-104279-choywmwd	219	9	analysis	analysis	NN
cord-104279-choywmwd	219	10	of	of	IN
cord-104279-choywmwd	219	11	eeFss	eeFss	NNP
cord-104279-choywmwd	219	12	-	-	HYPH
cord-104279-choywmwd	219	13	B	B	NNP
cord-104279-choywmwd	219	14	,	,	,
cord-104279-choywmwd	219	15	BB	BB	NNP
cord-104279-choywmwd	219	16	-	-	HYPH
cord-104279-choywmwd	219	17	Inv	Inv	NNP
cord-104279-choywmwd	219	18	,	,	,
cord-104279-choywmwd	219	19	and	and	CC
cord-104279-choywmwd	219	20	B	b	NN
cord-104279-choywmwd	219	21	-	-	HYPH
cord-104279-choywmwd	219	22	A	a	NN
cord-104279-choywmwd	219	23	-	-	HYPH
cord-104279-choywmwd	219	24	B	b	NN
cord-104279-choywmwd	219	25	,	,	,
cord-104279-choywmwd	219	26	demonstrate	demonstrate	VB
cord-104279-choywmwd	219	27	that	that	IN
cord-104279-choywmwd	219	28	,	,	,
cord-104279-choywmwd	219	29	aside	aside	RB
cord-104279-choywmwd	219	30	from	from	IN
cord-104279-choywmwd	219	31	a	a	DT
cord-104279-choywmwd	219	32	membrane	membrane	NN
cord-104279-choywmwd	219	33	anchor	anchor	NN
cord-104279-choywmwd	219	34	,	,	,
cord-104279-choywmwd	219	35	no	no	DT
cord-104279-choywmwd	219	36	single	single	JJ
cord-104279-choywmwd	219	37	domain	domain	NN
cord-104279-choywmwd	219	38	of	of	IN
cord-104279-choywmwd	219	39	DPAP	DPAP	NNP
cord-104279-choywmwd	219	40	B	B	NNP
cord-104279-choywmwd	219	41	is	be	VBZ
cord-104279-choywmwd	219	42	required	require	VBN
cord-104279-choywmwd	219	43	for	for	IN
cord-104279-choywmwd	219	44	transport	transport	NN
cord-104279-choywmwd	219	45	to	to	IN
cord-104279-choywmwd	219	46	the	the	DT
cord-104279-choywmwd	219	47	vacuole	vacuole	NN
cord-104279-choywmwd	219	48	.	.	.
cord-104279-choywmwd	220	1	*	*	NFP
cord-104279-choywmwd	220	2	For	for	IN
cord-104279-choywmwd	220	3	each	each	DT
cord-104279-choywmwd	220	4	construct	construct	NN
cord-104279-choywmwd	220	5	,	,	,
cord-104279-choywmwd	220	6	the	the	DT
cord-104279-choywmwd	220	7	percentages	percentage	NNS
cord-104279-choywmwd	220	8	refer	refer	VBP
cord-104279-choywmwd	220	9	to	to	IN
cord-104279-choywmwd	220	10	the	the	DT
cord-104279-choywmwd	220	11	fraction	fraction	NN
cord-104279-choywmwd	220	12	of	of	IN
cord-104279-choywmwd	220	13	stained	stained	JJ
cord-104279-choywmwd	220	14	cells	cell	NNS
cord-104279-choywmwd	220	15	that	that	WDT
cord-104279-choywmwd	220	16	exhibited	exhibit	VBD
cord-104279-choywmwd	220	17	staining	staining	NN
cord-104279-choywmwd	220	18	of	of	IN
cord-104279-choywmwd	220	19	a	a	DT
cord-104279-choywmwd	220	20	particular	particular	JJ
cord-104279-choywmwd	220	21	organelle	organelle	NN
cord-104279-choywmwd	220	22	.	.	.
cord-104279-choywmwd	221	1	Depending	depend	VBG
cord-104279-choywmwd	221	2	on	on	IN
cord-104279-choywmwd	221	3	the	the	DT
cord-104279-choywmwd	221	4	protein	protein	NN
cord-104279-choywmwd	221	5	being	be	VBG
cord-104279-choywmwd	221	6	monitored	monitor	VBN
cord-104279-choywmwd	221	7	,	,	,
cord-104279-choywmwd	221	8	some	some	DT
cord-104279-choywmwd	221	9	cells	cell	NNS
cord-104279-choywmwd	221	10	showed	show	VBD
cord-104279-choywmwd	221	11	staining	stain	VBG
cord-104279-choywmwd	221	12	of	of	IN
cord-104279-choywmwd	221	13	more	more	JJR
cord-104279-choywmwd	221	14	than	than	IN
cord-104279-choywmwd	221	15	one	one	CD
cord-104279-choywmwd	221	16	class	class	NN
cord-104279-choywmwd	221	17	of	of	IN
cord-104279-choywmwd	221	18	organdie	organdie	NN
cord-104279-choywmwd	221	19	;	;	:
cord-104279-choywmwd	221	20	thus	thus	RB
cord-104279-choywmwd	221	21	,	,	,
cord-104279-choywmwd	221	22	the	the	DT
cord-104279-choywmwd	221	23	percentages	percentage	NNS
cord-104279-choywmwd	221	24	for	for	IN
cord-104279-choywmwd	221	25	a	a	DT
cord-104279-choywmwd	221	26	given	give	VBN
cord-104279-choywmwd	221	27	protein	protein	NN
cord-104279-choywmwd	221	28	may	may	MD
cord-104279-choywmwd	221	29	add	add	VB
cord-104279-choywmwd	221	30	up	up	RP
cord-104279-choywmwd	221	31	to	to	TO
cord-104279-choywmwd	221	32	more	more	JJR
cord-104279-choywmwd	221	33	than	than	IN
cord-104279-choywmwd	221	34	100	100	CD
cord-104279-choywmwd	221	35	.	.	.
cord-104279-choywmwd	222	1	w	w	NNP
cord-104279-choywmwd	222	2	Vacuolar	Vacuolar	NNP
cord-104279-choywmwd	222	3	localization	localization	NN
cord-104279-choywmwd	222	4	was	be	VBD
cord-104279-choywmwd	222	5	determined	determine	VBN
cord-104279-choywmwd	222	6	both	both	CC
cord-104279-choywmwd	222	7	by	by	IN
cord-104279-choywmwd	222	8	coincidence	coincidence	NN
cord-104279-choywmwd	222	9	of	of	IN
cord-104279-choywmwd	222	10	staining	stain	VBG
cord-104279-choywmwd	222	11	with	with	IN
cord-104279-choywmwd	222	12	the	the	DT
cord-104279-choywmwd	222	13	vacuole	vacuole	NN
cord-104279-choywmwd	222	14	membrane	membrane	NN
cord-104279-choywmwd	222	15	as	as	IN
cord-104279-choywmwd	222	16	determined	determine	VBN
cord-104279-choywmwd	222	17	by	by	IN
cord-104279-choywmwd	222	18	Nomarski	Nomarski	NNP
cord-104279-choywmwd	222	19	optics	optic	NNS
cord-104279-choywmwd	222	20	,	,	,
cord-104279-choywmwd	222	21	and	and	CC
cord-104279-choywmwd	222	22	by	by	IN
cord-104279-choywmwd	222	23	co	co	NN
cord-104279-choywmwd	222	24	-	-	NN
cord-104279-choywmwd	222	25	localization	localization	NN
cord-104279-choywmwd	222	26	with	with	IN
cord-104279-choywmwd	222	27	Vat2p	Vat2p	NNP
cord-104279-choywmwd	222	28	.	.	.
cord-104279-choywmwd	223	1	I	-PRON-	PRP
cord-104279-choywmwd	223	2	)	)	-RRB-
cord-104279-choywmwd	224	1	Golgi	Golgi	NNP
cord-104279-choywmwd	224	2	localization	localization	NN
cord-104279-choywmwd	224	3	was	be	VBD
cord-104279-choywmwd	224	4	defined	define	VBN
cord-104279-choywmwd	224	5	as	as	IN
cord-104279-choywmwd	224	6	punctate	punctate	NNP
cord-104279-choywmwd	224	7	,	,	,
cord-104279-choywmwd	224	8	non	non	JJ
cord-104279-choywmwd	224	9	-	-	JJ
cord-104279-choywmwd	224	10	vacuolar	vacuolar	JJ
cord-104279-choywmwd	224	11	,	,	,
cord-104279-choywmwd	224	12	and	and	CC
cord-104279-choywmwd	224	13	non	non	JJ
cord-104279-choywmwd	224	14	-	-	JJ
cord-104279-choywmwd	224	15	ER	er	JJ
cord-104279-choywmwd	224	16	staining	staining	NN
cord-104279-choywmwd	224	17	,	,	,
cord-104279-choywmwd	224	18	characteristic	characteristic	NN
cord-104279-choywmwd	224	19	of	of	IN
cord-104279-choywmwd	224	20	DPAP	DPAP	NNP
cord-104279-choywmwd	224	21	A	A	NNP
cord-104279-choywmwd	224	22	,	,	,
cord-104279-choywmwd	224	23	Kex2p	Kex2p	NNP
cord-104279-choywmwd	224	24	,	,	,
cord-104279-choywmwd	224	25	and	and	CC
cord-104279-choywmwd	224	26	Kexlp	Kexlp	NNP
cord-104279-choywmwd	224	27	(	(	-LRB-
cord-104279-choywmwd	224	28	Cooper	Cooper	NNP
cord-104279-choywmwd	224	29	and	and	CC
cord-104279-choywmwd	224	30	Bussey	Bussey	NNP
cord-104279-choywmwd	224	31	,	,	,
cord-104279-choywmwd	224	32	manuscript	manuscript	NN
cord-104279-choywmwd	224	33	submitted	submit	VBN
cord-104279-choywmwd	224	34	for	for	IN
cord-104279-choywmwd	224	35	publication	publication	NN
cord-104279-choywmwd	224	36	)	)	-RRB-
cord-104279-choywmwd	224	37	.	.	.
cord-104279-choywmwd	225	1	*	*	NFP
cord-104279-choywmwd	225	2	*	*	NFP
cord-104279-choywmwd	225	3	ER	er	NN
cord-104279-choywmwd	225	4	localization	localization	NN
cord-104279-choywmwd	225	5	was	be	VBD
cord-104279-choywmwd	225	6	determined	determine	VBN
cord-104279-choywmwd	225	7	by	by	IN
cord-104279-choywmwd	225	8	staining	stain	VBG
cord-104279-choywmwd	225	9	of	of	IN
cord-104279-choywmwd	225	10	perinuclear	perinuclear	NNP
cord-104279-choywmwd	225	11	(	(	-LRB-
cord-104279-choywmwd	225	12	as	as	IN
cord-104279-choywmwd	225	13	determined	determine	VBN
cord-104279-choywmwd	225	14	by	by	IN
cord-104279-choywmwd	225	15	DAPI	DAPI	NNP
cord-104279-choywmwd	225	16	staining	staining	NN
cord-104279-choywmwd	225	17	of	of	IN
cord-104279-choywmwd	225	18	nuclei	nucleus	NNS
cord-104279-choywmwd	225	19	)	)	-RRB-
cord-104279-choywmwd	225	20	and	and	CC
cord-104279-choywmwd	225	21	extended	extended	JJ
cord-104279-choywmwd	225	22	cisternal	cisternal	JJ
cord-104279-choywmwd	225	23	structures	structure	NNS
cord-104279-choywmwd	225	24	.	.	.
cord-104279-choywmwd	226	1	Several	several	JJ
cord-104279-choywmwd	226	2	different	different	JJ
cord-104279-choywmwd	226	3	models	model	NNS
cord-104279-choywmwd	226	4	can	can	MD
cord-104279-choywmwd	226	5	explain	explain	VB
cord-104279-choywmwd	226	6	the	the	DT
cord-104279-choywmwd	226	7	data	datum	NNS
cord-104279-choywmwd	226	8	presented	present	VBN
cord-104279-choywmwd	226	9	above	above	RB
cord-104279-choywmwd	226	10	(	(	-LRB-
cord-104279-choywmwd	226	11	see	see	VB
cord-104279-choywmwd	226	12	Discussion	Discussion	NNP
cord-104279-choywmwd	226	13	)	)	-RRB-
cord-104279-choywmwd	226	14	,	,	,
cord-104279-choywmwd	226	15	including	include	VBG
cord-104279-choywmwd	226	16	a	a	DT
cord-104279-choywmwd	226	17	simple	simple	JJ
cord-104279-choywmwd	226	18	model	model	NN
cord-104279-choywmwd	226	19	in	in	IN
cord-104279-choywmwd	226	20	which	which	WDT
cord-104279-choywmwd	226	21	the	the	DT
cord-104279-choywmwd	226	22	vacuolar	vacuolar	NNP
cord-104279-choywmwd	226	23	membrane	membrane	NN
cord-104279-choywmwd	226	24	,	,	,
cord-104279-choywmwd	226	25	not	not	RB
cord-104279-choywmwd	226	26	the	the	DT
cord-104279-choywmwd	226	27	plasma	plasma	NN
cord-104279-choywmwd	226	28	membrane	membrane	NN
cord-104279-choywmwd	226	29	,	,	,
cord-104279-choywmwd	226	30	is	be	VBZ
cord-104279-choywmwd	226	31	the	the	DT
cord-104279-choywmwd	226	32	default	default	NN
cord-104279-choywmwd	226	33	compartment	compartment	NN
cord-104279-choywmwd	226	34	for	for	IN
cord-104279-choywmwd	226	35	membrane	membrane	NN
cord-104279-choywmwd	226	36	proteins	protein	NNS
cord-104279-choywmwd	226	37	of	of	IN
cord-104279-choywmwd	226	38	the	the	DT
cord-104279-choywmwd	226	39	yeast	yeast	NN
cord-104279-choywmwd	226	40	secretory	secretory	NN
cord-104279-choywmwd	226	41	pathway	pathway	NN
cord-104279-choywmwd	226	42	.	.	.
cord-104279-choywmwd	227	1	To	to	TO
cord-104279-choywmwd	227	2	distinguish	distinguish	VB
cord-104279-choywmwd	227	3	among	among	IN
cord-104279-choywmwd	227	4	these	these	DT
cord-104279-choywmwd	227	5	models	model	NNS
cord-104279-choywmwd	227	6	,	,	,
cord-104279-choywmwd	227	7	we	-PRON-	PRP
cord-104279-choywmwd	227	8	analyzed	analyze	VBD
cord-104279-choywmwd	227	9	the	the	DT
cord-104279-choywmwd	227	10	retention	retention	NN
cord-104279-choywmwd	227	11	signal	signal	NN
cord-104279-choywmwd	227	12	of	of	IN
cord-104279-choywmwd	227	13	the	the	DT
cord-104279-choywmwd	227	14	Golgi	Golgi	NNP
cord-104279-choywmwd	227	15	membrane	membrane	NN
cord-104279-choywmwd	227	16	protein	protein	NN
cord-104279-choywmwd	227	17	,	,	,
cord-104279-choywmwd	227	18	DPAP	DPAP	NNP
cord-104279-choywmwd	227	19	A.	A.	NNP
cord-104279-choywmwd	227	20	Mutations	Mutations	NNPS
cord-104279-choywmwd	227	21	in	in	IN
cord-104279-choywmwd	227	22	the	the	DT
cord-104279-choywmwd	227	23	retention	retention	NN
cord-104279-choywmwd	227	24	signal	signal	NN
cord-104279-choywmwd	227	25	of	of	IN
cord-104279-choywmwd	227	26	this	this	DT
cord-104279-choywmwd	227	27	protein	protein	NN
cord-104279-choywmwd	227	28	should	should	MD
cord-104279-choywmwd	227	29	result	result	VB
cord-104279-choywmwd	227	30	in	in	IN
cord-104279-choywmwd	227	31	DPAP	DPAP	NNP
cord-104279-choywmwd	227	32	A	a	DT
cord-104279-choywmwd	227	33	becoming	become	VBG
cord-104279-choywmwd	227	34	localized	localize	VBN
cord-104279-choywmwd	227	35	to	to	IN
cord-104279-choywmwd	227	36	the	the	DT
cord-104279-choywmwd	227	37	default	default	NN
cord-104279-choywmwd	227	38	compartment	compartment	NN
cord-104279-choywmwd	227	39	for	for	IN
cord-104279-choywmwd	227	40	membrane	membrane	NN
cord-104279-choywmwd	227	41	proteins	protein	NNS
cord-104279-choywmwd	227	42	.	.	.
cord-104279-choywmwd	228	1	DPAP	DPAP	NNP
cord-104279-choywmwd	228	2	A	A	NNP
cord-104279-choywmwd	228	3	is	be	VBZ
cord-104279-choywmwd	228	4	a	a	DT
cord-104279-choywmwd	228	5	protein	protein	NN
cord-104279-choywmwd	228	6	that	that	WDT
cord-104279-choywmwd	228	7	resides	reside	VBZ
cord-104279-choywmwd	228	8	in	in	IN
cord-104279-choywmwd	228	9	the	the	DT
cord-104279-choywmwd	228	10	Golgi	Golgi	NNP
cord-104279-choywmwd	228	11	apparatus	apparatus	NN
cord-104279-choywmwd	228	12	,	,	,
cord-104279-choywmwd	228	13	where	where	WRB
cord-104279-choywmwd	228	14	it	-PRON-	PRP
cord-104279-choywmwd	228	15	processes	process	VBZ
cord-104279-choywmwd	228	16	the	the	DT
cord-104279-choywmwd	228	17	c~-factor	c~-factor	NN
cord-104279-choywmwd	228	18	precursor	precursor	NN
cord-104279-choywmwd	228	19	polypeptide	polypeptide	NN
cord-104279-choywmwd	228	20	(	(	-LRB-
cord-104279-choywmwd	228	21	Julius	Julius	NNP
cord-104279-choywmwd	228	22	et	et	NNP
cord-104279-choywmwd	228	23	al	al	NNP
cord-104279-choywmwd	228	24	.	.	NNP
cord-104279-choywmwd	228	25	,	,	,
cord-104279-choywmwd	228	26	1983	1983	CD
cord-104279-choywmwd	228	27	)	)	-RRB-
cord-104279-choywmwd	228	28	.	.	.
cord-104279-choywmwd	229	1	The	the	DT
cord-104279-choywmwd	229	2	structural	structural	JJ
cord-104279-choywmwd	229	3	gene	gene	NN
cord-104279-choywmwd	229	4	for	for	IN
cord-104279-choywmwd	229	5	DPAP	DPAP	NNP
cord-104279-choywmwd	229	6	A	A	NNP
cord-104279-choywmwd	229	7	(	(	-LRB-
cord-104279-choywmwd	229	8	STE23	STE23	NNP
cord-104279-choywmwd	229	9	)	)	-RRB-
cord-104279-choywmwd	229	10	has	have	VBZ
cord-104279-choywmwd	229	11	been	be	VBN
cord-104279-choywmwd	229	12	cloned	clone	VBN
cord-104279-choywmwd	229	13	and	and	CC
cord-104279-choywmwd	229	14	sequenced	sequence	VBN
cord-104279-choywmwd	230	1	(	(	-LRB-
cord-104279-choywmwd	230	2	Julius	Julius	NNP
cord-104279-choywmwd	230	3	et	et	NNP
cord-104279-choywmwd	230	4	al	al	NNP
cord-104279-choywmwd	230	5	.	.	NNP
cord-104279-choywmwd	230	6	,	,	,
cord-104279-choywmwd	230	7	1983	1983	CD
cord-104279-choywmwd	231	1	;	;	:
cord-104279-choywmwd	231	2	C.	C.	NNP
cord-104279-choywmwd	231	3	A.	A.	NNP
cord-104279-choywmwd	231	4	Flanagun	Flanagun	NNP
cord-104279-choywmwd	231	5	,	,	,
cord-104279-choywmwd	231	6	D.	D.	NNP
cord-104279-choywmwd	231	7	A.	A.	NNP
cord-104279-choywmwd	231	8	Barnes	Barnes	NNP
cord-104279-choywmwd	231	9	,	,	,
cord-104279-choywmwd	231	10	M.	M.	NNP
cord-104279-choywmwd	231	11	C.	C.	NNP
cord-104279-choywmwd	231	12	Flessel	Flessel	NNP
cord-104279-choywmwd	231	13	,	,	,
cord-104279-choywmwd	231	14	and	and	CC
cord-104279-choywmwd	231	15	J.	J.	NNP
cord-104279-choywmwd	231	16	Thorner	Thorner	NNP
cord-104279-choywmwd	231	17	,	,	,
cord-104279-choywmwd	231	18	manuscript	manuscript	NN
cord-104279-choywmwd	231	19	submitted	submit	VBN
cord-104279-choywmwd	231	20	for	for	IN
cord-104279-choywmwd	231	21	publication	publication	NN
cord-104279-choywmwd	231	22	)	)	-RRB-
cord-104279-choywmwd	231	23	,	,	,
cord-104279-choywmwd	231	24	and	and	CC
cord-104279-choywmwd	231	25	the	the	DT
cord-104279-choywmwd	231	26	predicted	predict	VBN
cord-104279-choywmwd	231	27	structure	structure	NN
cord-104279-choywmwd	231	28	of	of	IN
cord-104279-choywmwd	231	29	DPAP	DPAP	NNP
cord-104279-choywmwd	231	30	A	A	NNP
cord-104279-choywmwd	231	31	is	be	VBZ
cord-104279-choywmwd	231	32	similar	similar	JJ
cord-104279-choywmwd	231	33	to	to	IN
cord-104279-choywmwd	231	34	that	that	DT
cord-104279-choywmwd	231	35	of	of	IN
cord-104279-choywmwd	231	36	DPAP	DPAP	NNP
cord-104279-choywmwd	231	37	B	B	NNP
cord-104279-choywmwd	231	38	in	in	IN
cord-104279-choywmwd	231	39	several	several	JJ
cord-104279-choywmwd	231	40	regards	regard	NNS
cord-104279-choywmwd	231	41	.	.	.
cord-104279-choywmwd	232	1	Both	both	DT
cord-104279-choywmwd	232	2	are	be	VBP
cord-104279-choywmwd	232	3	type	type	NN
cord-104279-choywmwd	232	4	II	II	NNP
cord-104279-choywmwd	232	5	integral	integral	JJ
cord-104279-choywmwd	232	6	membrane	membrane	NN
cord-104279-choywmwd	232	7	proteins	protein	NNS
cord-104279-choywmwd	232	8	,	,	,
cord-104279-choywmwd	232	9	and	and	CC
cord-104279-choywmwd	232	10	both	both	DT
cord-104279-choywmwd	232	11	have	have	VBP
cord-104279-choywmwd	232	12	lumenal	lumenal	JJ
cord-104279-choywmwd	232	13	enzymatic	enzymatic	JJ
cord-104279-choywmwd	232	14	domains	domain	NNS
cord-104279-choywmwd	232	15	of	of	IN
cord-104279-choywmwd	232	16	,	,	,
cord-104279-choywmwd	233	1	x,800	x,800	NN
cord-104279-choywmwd	233	2	residues	residue	NNS
cord-104279-choywmwd	233	3	that	that	WDT
cord-104279-choywmwd	233	4	share	share	VBP
cord-104279-choywmwd	233	5	a	a	DT
cord-104279-choywmwd	233	6	high	high	JJ
cord-104279-choywmwd	233	7	degree	degree	NN
cord-104279-choywmwd	233	8	of	of	IN
cord-104279-choywmwd	233	9	sequence	sequence	NN
cord-104279-choywmwd	233	10	identity	identity	NN
cord-104279-choywmwd	233	11	,	,	,
cord-104279-choywmwd	233	12	including	include	VBG
cord-104279-choywmwd	233	13	48	48	CD
cord-104279-choywmwd	233	14	%	%	NN
cord-104279-choywmwd	233	15	identical	identical	JJ
cord-104279-choywmwd	233	16	residues	residue	NNS
cord-104279-choywmwd	233	17	over	over	IN
cord-104279-choywmwd	233	18	the	the	DT
cord-104279-choywmwd	233	19	COOH	COOH	NNP
cord-104279-choywmwd	233	20	-	-	HYPH
cord-104279-choywmwd	233	21	terminal	terminal	NN
cord-104279-choywmwd	233	22	250	250	CD
cord-104279-choywmwd	233	23	residues	residue	NNS
cord-104279-choywmwd	233	24	(	(	-LRB-
cord-104279-choywmwd	233	25	Fig	Fig	NNP
cord-104279-choywmwd	233	26	.	.	.
cord-104279-choywmwd	233	27	1	1	CD
cord-104279-choywmwd	233	28	A	a	NN
cord-104279-choywmwd	233	29	)	)	-RRB-
cord-104279-choywmwd	234	1	;	;	:
cord-104279-choywmwd	234	2	however	however	RB
cord-104279-choywmwd	234	3	,	,	,
cord-104279-choywmwd	234	4	there	there	EX
cord-104279-choywmwd	234	5	is	be	VBZ
cord-104279-choywmwd	234	6	no	no	DT
cord-104279-choywmwd	234	7	significant	significant	JJ
cord-104279-choywmwd	234	8	sequence	sequence	NN
cord-104279-choywmwd	234	9	similarity	similarity	NN
cord-104279-choywmwd	234	10	between	between	IN
cord-104279-choywmwd	234	11	the	the	DT
cord-104279-choywmwd	234	12	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	234	13	and	and	CC
cord-104279-choywmwd	234	14	transmembrane	transmembrane	JJ
cord-104279-choywmwd	234	15	domains	domain	NNS
cord-104279-choywmwd	234	16	of	of	IN
cord-104279-choywmwd	234	17	DPAP	DPAP	NNP
cord-104279-choywmwd	234	18	A	A	NNP
cord-104279-choywmwd	234	19	and	and	CC
cord-104279-choywmwd	234	20	DPAP	DPAP	NNP
cord-104279-choywmwd	234	21	B.	B.	NNP
cord-104279-choywmwd	235	1	To	to	TO
cord-104279-choywmwd	235	2	assess	assess	VB
cord-104279-choywmwd	235	3	the	the	DT
cord-104279-choywmwd	235	4	subcelhlar	subcelhlar	JJ
cord-104279-choywmwd	235	5	distribution	distribution	NN
cord-104279-choywmwd	235	6	of	of	IN
cord-104279-choywmwd	235	7	DPAP	DPAP	NNP
cord-104279-choywmwd	235	8	A	A	NNP
cord-104279-choywmwd	235	9	,	,	,
cord-104279-choywmwd	235	10	an	an	DT
cord-104279-choywmwd	235	11	antibody	antibody	NN
cord-104279-choywmwd	235	12	specific	specific	JJ
cord-104279-choywmwd	235	13	for	for	IN
cord-104279-choywmwd	235	14	DPAP	DPAP	NNP
cord-104279-choywmwd	235	15	A	A	NNP
cord-104279-choywmwd	235	16	was	be	VBD
cord-104279-choywmwd	235	17	generated	generate	VBN
cord-104279-choywmwd	235	18	(	(	-LRB-
cord-104279-choywmwd	235	19	see	see	VB
cord-104279-choywmwd	235	20	Materials	material	NNS
cord-104279-choywmwd	235	21	and	and	CC
cord-104279-choywmwd	235	22	Methods	Methods	NNPS
cord-104279-choywmwd	235	23	)	)	-RRB-
cord-104279-choywmwd	235	24	.	.	.
cord-104279-choywmwd	236	1	The	the	DT
cord-104279-choywmwd	236	2	specificity	specificity	NN
cord-104279-choywmwd	236	3	of	of	IN
cord-104279-choywmwd	236	4	the	the	DT
cord-104279-choywmwd	236	5	antibody	antibody	NN
cord-104279-choywmwd	236	6	is	be	VBZ
cord-104279-choywmwd	236	7	demonst	demonst	RB
cord-104279-choywmwd	236	8	-	-	HYPH
cord-104279-choywmwd	236	9	rated	rate	VBN
cord-104279-choywmwd	236	10	in	in	IN
cord-104279-choywmwd	236	11	Fig	Fig	NNP
cord-104279-choywmwd	236	12	.	.	.
cord-104279-choywmwd	237	1	6	6	LS
cord-104279-choywmwd	237	2	.	.	.
cord-104279-choywmwd	238	1	Immunoprecipitation	immunoprecipitation	NN
cord-104279-choywmwd	238	2	of	of	IN
cord-104279-choywmwd	238	3	DPAP	DPAP	NNP
cord-104279-choywmwd	238	4	A	A	NNP
cord-104279-choywmwd	238	5	was	be	VBD
cord-104279-choywmwd	238	6	carried	carry	VBN
cord-104279-choywmwd	238	7	out	out	RP
cord-104279-choywmwd	238	8	with	with	IN
cord-104279-choywmwd	238	9	35S	35S	NNP
cord-104279-choywmwd	238	10	-	-	HYPH
cord-104279-choywmwd	238	11	labeled	label	VBN
cord-104279-choywmwd	238	12	cells	cell	NNS
cord-104279-choywmwd	238	13	that	that	WDT
cord-104279-choywmwd	238	14	varied	varied	JJ
cord-104279-choywmwd	238	15	with	with	IN
cord-104279-choywmwd	238	16	regard	regard	NN
cord-104279-choywmwd	238	17	to	to	IN
cord-104279-choywmwd	238	18	the	the	DT
cord-104279-choywmwd	238	19	dosage	dosage	NN
cord-104279-choywmwd	238	20	of	of	IN
cord-104279-choywmwd	238	21	the	the	DT
cord-104279-choywmwd	238	22	STE/3	STE/3	NNP
cord-104279-choywmwd	238	23	gene	gene	NN
cord-104279-choywmwd	238	24	.	.	.
cord-104279-choywmwd	239	1	SDS	SDS	NNP
cord-104279-choywmwd	239	2	-	-	HYPH
cord-104279-choywmwd	239	3	PAGE	PAGE	NNP
cord-104279-choywmwd	239	4	and	and	CC
cord-104279-choywmwd	239	5	fluorography	fluorography	NN
cord-104279-choywmwd	239	6	showed	show	VBD
cord-104279-choywmwd	239	7	that	that	IN
cord-104279-choywmwd	239	8	the	the	DT
cord-104279-choywmwd	239	9	antibody	antibody	NN
cord-104279-choywmwd	239	10	immunoprecipitated	immunoprecipitate	VBD
cord-104279-choywmwd	239	11	a	a	DT
cord-104279-choywmwd	239	12	protein	protein	NN
cord-104279-choywmwd	239	13	of	of	IN
cord-104279-choywmwd	239	14	112	112	CD
cord-104279-choywmwd	239	15	kD	kD	VBN
cord-104279-choywmwd	239	16	from	from	IN
cord-104279-choywmwd	239	17	wild	wild	JJ
cord-104279-choywmwd	239	18	type	type	NN
cord-104279-choywmwd	239	19	cells	cell	NNS
cord-104279-choywmwd	239	20	but	but	CC
cord-104279-choywmwd	239	21	not	not	RB
cord-104279-choywmwd	239	22	from	from	IN
cord-104279-choywmwd	239	23	ste13A	ste13A	NNP
cord-104279-choywmwd	239	24	ceils	ceil	NNS
cord-104279-choywmwd	239	25	,	,	,
cord-104279-choywmwd	239	26	and	and	CC
cord-104279-choywmwd	239	27	that	that	IN
cord-104279-choywmwd	239	28	this	this	DT
cord-104279-choywmwd	239	29	polypeptide	polypeptide	NN
cord-104279-choywmwd	239	30	was	be	VBD
cord-104279-choywmwd	239	31	overproduced	overproduce	VBN
cord-104279-choywmwd	239	32	~20-fold	~20-fold	NN
cord-104279-choywmwd	239	33	in	in	IN
cord-104279-choywmwd	239	34	a	a	DT
cord-104279-choywmwd	239	35	strain	strain	NN
cord-104279-choywmwd	239	36	containing	contain	VBG
cord-104279-choywmwd	239	37	the	the	DT
cord-104279-choywmwd	239	38	STE/3	STE/3	NNP
cord-104279-choywmwd	239	39	gene	gene	NN
cord-104279-choywmwd	239	40	on	on	IN
cord-104279-choywmwd	239	41	a	a	DT
cord-104279-choywmwd	239	42	2t~	2t~	CD
cord-104279-choywmwd	239	43	plasmid	plasmid	NN
cord-104279-choywmwd	239	44	(	(	-LRB-
cord-104279-choywmwd	239	45	Fig	Fig	NNP
cord-104279-choywmwd	239	46	.	.	.
cord-104279-choywmwd	239	47	6	6	CD
cord-104279-choywmwd	239	48	A	a	NN
cord-104279-choywmwd	239	49	)	)	-RRB-
cord-104279-choywmwd	239	50	.	.	.
cord-104279-choywmwd	240	1	A	a	DT
cord-104279-choywmwd	240	2	protein	protein	NN
cord-104279-choywmwd	240	3	of	of	IN
cord-104279-choywmwd	240	4	*	*	NFP
cord-104279-choywmwd	240	5	40	40	CD
cord-104279-choywmwd	240	6	kD	kD	NNP
cord-104279-choywmwd	240	7	was	be	VBD
cord-104279-choywmwd	240	8	also	also	RB
cord-104279-choywmwd	240	9	immunoprecipitated	immunoprecipitate	VBN
cord-104279-choywmwd	240	10	;	;	:
cord-104279-choywmwd	240	11	however	however	RB
cord-104279-choywmwd	240	12	,	,	,
cord-104279-choywmwd	240	13	the	the	DT
cord-104279-choywmwd	240	14	level	level	NN
cord-104279-choywmwd	240	15	of	of	IN
cord-104279-choywmwd	240	16	the	the	DT
cord-104279-choywmwd	240	17	40-kD	40-kd	CD
cord-104279-choywmwd	240	18	protein	protein	NN
cord-104279-choywmwd	240	19	did	do	VBD
cord-104279-choywmwd	240	20	not	not	RB
cord-104279-choywmwd	240	21	vary	vary	VB
cord-104279-choywmwd	240	22	with	with	IN
cord-104279-choywmwd	240	23	the	the	DT
cord-104279-choywmwd	240	24	dosage	dosage	NN
cord-104279-choywmwd	240	25	of	of	IN
cord-104279-choywmwd	240	26	STE/3	STE/3	NNP
cord-104279-choywmwd	240	27	.	.	.
cord-104279-choywmwd	241	1	The	the	DT
cord-104279-choywmwd	241	2	STE/3	STE/3	NNP
cord-104279-choywmwd	241	3	DNA	dna	NN
cord-104279-choywmwd	241	4	sequence	sequence	NN
cord-104279-choywmwd	241	5	predicts	predict	VBZ
cord-104279-choywmwd	241	6	three	three	CD
cord-104279-choywmwd	241	7	possible	possible	JJ
cord-104279-choywmwd	241	8	sites	site	NNS
cord-104279-choywmwd	241	9	for	for	IN
cord-104279-choywmwd	241	10	addition	addition	NN
cord-104279-choywmwd	241	11	of	of	IN
cord-104279-choywmwd	241	12	N	n	NN
cord-104279-choywmwd	241	13	-	-	HYPH
cord-104279-choywmwd	241	14	linked	link	VBN
cord-104279-choywmwd	241	15	carbohydrate	carbohydrate	NN
cord-104279-choywmwd	242	1	(	(	-LRB-
cord-104279-choywmwd	242	2	C.	C.	NNP
cord-104279-choywmwd	242	3	A.	A.	NNP
cord-104279-choywmwd	242	4	Flanagan	Flanagan	NNP
cord-104279-choywmwd	242	5	,	,	,
cord-104279-choywmwd	242	6	D.	D.	NNP
cord-104279-choywmwd	242	7	A.	A.	NNP
cord-104279-choywmwd	242	8	Barnes	Barnes	NNP
cord-104279-choywmwd	242	9	,	,	,
cord-104279-choywmwd	242	10	M.	M.	NNP
cord-104279-choywmwd	242	11	C.	C.	NNP
cord-104279-choywmwd	242	12	Flessel	Flessel	NNP
cord-104279-choywmwd	242	13	,	,	,
cord-104279-choywmwd	242	14	and	and	CC
cord-104279-choywmwd	242	15	J.	J.	NNP
cord-104279-choywmwd	242	16	Thorner	Thorner	NNP
cord-104279-choywmwd	242	17	,	,	,
cord-104279-choywmwd	242	18	manuscript	manuscript	NN
cord-104279-choywmwd	242	19	submitted	submit	VBN
cord-104279-choywmwd	242	20	for	for	IN
cord-104279-choywmwd	242	21	publication	publication	NN
cord-104279-choywmwd	242	22	)	)	-RRB-
cord-104279-choywmwd	242	23	.	.	.
cord-104279-choywmwd	243	1	Treatment	treatment	NN
cord-104279-choywmwd	243	2	of	of	IN
cord-104279-choywmwd	243	3	DPAP	DPAP	NNP
cord-104279-choywmwd	243	4	A	A	NNP
cord-104279-choywmwd	243	5	with	with	IN
cord-104279-choywmwd	243	6	Endo	Endo	NNP
cord-104279-choywmwd	243	7	H	H	NNP
cord-104279-choywmwd	243	8	resulted	result	VBD
cord-104279-choywmwd	243	9	in	in	IN
cord-104279-choywmwd	243	10	an	an	DT
cord-104279-choywmwd	243	11	~,5	~,5	CD
cord-104279-choywmwd	243	12	kD	kd	XX
cord-104279-choywmwd	243	13	decrease	decrease	NN
cord-104279-choywmwd	243	14	in	in	IN
cord-104279-choywmwd	243	15	apparent	apparent	JJ
cord-104279-choywmwd	243	16	molecular	molecular	JJ
cord-104279-choywmwd	243	17	weight	weight	NN
cord-104279-choywmwd	244	1	(	(	-LRB-
cord-104279-choywmwd	244	2	Fig	Fig	NNP
cord-104279-choywmwd	244	3	.	.	.
cord-104279-choywmwd	244	4	6	6	CD
cord-104279-choywmwd	244	5	B	b	NN
cord-104279-choywmwd	244	6	)	)	-RRB-
cord-104279-choywmwd	244	7	,	,	,
cord-104279-choywmwd	244	8	suggesting	suggest	VBG
cord-104279-choywmwd	244	9	that	that	IN
cord-104279-choywmwd	244	10	at	at	RB
cord-104279-choywmwd	244	11	least	least	RBS
cord-104279-choywmwd	244	12	two	two	CD
cord-104279-choywmwd	244	13	of	of	IN
cord-104279-choywmwd	244	14	the	the	DT
cord-104279-choywmwd	244	15	three	three	CD
cord-104279-choywmwd	244	16	Asn	Asn	NNP
cord-104279-choywmwd	244	17	-	-	HYPH
cord-104279-choywmwd	244	18	X	X	NNP
cord-104279-choywmwd	244	19	-	-	JJ
cord-104279-choywmwd	244	20	Ser	Ser	NNP
cord-104279-choywmwd	244	21	/	/	SYM
cord-104279-choywmwd	244	22	Thr	Thr	NNP
cord-104279-choywmwd	244	23	sites	site	NNS
cord-104279-choywmwd	244	24	of	of	IN
cord-104279-choywmwd	244	25	DPAP	DPAP	NNP
cord-104279-choywmwd	244	26	A	A	NNP
cord-104279-choywmwd	244	27	were	be	VBD
cord-104279-choywmwd	244	28	modified	modify	VBN
cord-104279-choywmwd	244	29	,	,	,
cord-104279-choywmwd	244	30	assuming	assume	VBG
cord-104279-choywmwd	244	31	that	that	IN
cord-104279-choywmwd	244	32	the	the	DT
cord-104279-choywmwd	244	33	core	core	NN
cord-104279-choywmwd	244	34	oligosaccharides	oligosaccharide	NNS
cord-104279-choywmwd	244	35	added	add	VBN
cord-104279-choywmwd	244	36	were	be	VBD
cord-104279-choywmwd	244	37	of	of	IN
cord-104279-choywmwd	244	38	the	the	DT
cord-104279-choywmwd	244	39	typical	typical	JJ
cord-104279-choywmwd	244	40	structure	structure	NN
cord-104279-choywmwd	244	41	(	(	-LRB-
cord-104279-choywmwd	244	42	Kukuruzinska	kukuruzinska	FW
cord-104279-choywmwd	244	43	et	et	FW
cord-104279-choywmwd	244	44	al	al	NNP
cord-104279-choywmwd	244	45	.	.	NNP
cord-104279-choywmwd	244	46	,	,	,
cord-104279-choywmwd	244	47	1987	1987	CD
cord-104279-choywmwd	244	48	)	)	-RRB-
cord-104279-choywmwd	244	49	,	,	,
cord-104279-choywmwd	244	50	and	and	CC
cord-104279-choywmwd	244	51	that	that	IN
cord-104279-choywmwd	244	52	the	the	DT
cord-104279-choywmwd	244	53	carbohydrate	carbohydrate	NN
cord-104279-choywmwd	244	54	moieties	moiety	NNS
cord-104279-choywmwd	244	55	were	be	VBD
cord-104279-choywmwd	244	56	only	only	RB
cord-104279-choywmwd	244	57	slightly	slightly	RB
cord-104279-choywmwd	244	58	modified	modify	VBN
cord-104279-choywmwd	244	59	in	in	IN
cord-104279-choywmwd	244	60	the	the	DT
cord-104279-choywmwd	244	61	Golgi	Golgi	NNP
cord-104279-choywmwd	244	62	apparatus	apparatus	NN
cord-104279-choywmwd	244	63	,	,	,
cord-104279-choywmwd	244	64	as	as	IN
cord-104279-choywmwd	244	65	is	be	VBZ
cord-104279-choywmwd	244	66	the	the	DT
cord-104279-choywmwd	244	67	case	case	NN
cord-104279-choywmwd	244	68	for	for	IN
cord-104279-choywmwd	244	69	the	the	DT
cord-104279-choywmwd	244	70	glycosyl	glycosyl	JJ
cord-104279-choywmwd	244	71	groups	group	NNS
cord-104279-choywmwd	244	72	of	of	IN
cord-104279-choywmwd	244	73	Kex2p	Kex2p	NNP
cord-104279-choywmwd	245	1	(	(	-LRB-
cord-104279-choywmwd	245	2	Fuller	Fuller	NNP
cord-104279-choywmwd	245	3	et	et	NNP
cord-104279-choywmwd	245	4	al	al	NNP
cord-104279-choywmwd	245	5	.	.	NNP
cord-104279-choywmwd	245	6	,	,	,
cord-104279-choywmwd	245	7	1989a	1989a	CD
cord-104279-choywmwd	245	8	,	,	,
cord-104279-choywmwd	245	9	b	b	LS
cord-104279-choywmwd	245	10	)	)	-RRB-
cord-104279-choywmwd	245	11	and	and	CC
cord-104279-choywmwd	245	12	Kexlp	Kexlp	NNP
cord-104279-choywmwd	245	13	(	(	-LRB-
cord-104279-choywmwd	245	14	Cooper	Cooper	NNP
cord-104279-choywmwd	245	15	and	and	CC
cord-104279-choywmwd	245	16	Bussey	Bussey	NNP
cord-104279-choywmwd	245	17	,	,	,
cord-104279-choywmwd	245	18	1989	1989	CD
cord-104279-choywmwd	245	19	)	)	-RRB-
cord-104279-choywmwd	245	20	.	.	.
cord-104279-choywmwd	246	1	The	the	DT
cord-104279-choywmwd	246	2	apparent	apparent	JJ
cord-104279-choywmwd	246	3	molecular	molecular	JJ
cord-104279-choywmwd	246	4	weight	weight	NN
cord-104279-choywmwd	246	5	of	of	IN
cord-104279-choywmwd	246	6	deglycosylated	deglycosylate	VBN
cord-104279-choywmwd	246	7	DPAP	DPAP	NNP
cord-104279-choywmwd	246	8	A	A	NNP
cord-104279-choywmwd	246	9	,	,	,
cord-104279-choywmwd	246	10	107	107	CD
cord-104279-choywmwd	246	11	kD	kd	ADD
cord-104279-choywmwd	246	12	,	,	,
cord-104279-choywmwd	246	13	is	be	VBZ
cord-104279-choywmwd	246	14	consistent	consistent	JJ
cord-104279-choywmwd	246	15	with	with	IN
cord-104279-choywmwd	246	16	the	the	DT
cord-104279-choywmwd	246	17	molecular	molecular	JJ
cord-104279-choywmwd	246	18	weight	weight	NN
cord-104279-choywmwd	246	19	of	of	IN
cord-104279-choywmwd	246	20	DPAP	DPAP	NNP
cord-104279-choywmwd	246	21	A	A	NNP
cord-104279-choywmwd	246	22	predicted	predict	VBN
cord-104279-choywmwd	246	23	from	from	IN
cord-104279-choywmwd	246	24	the	the	DT
cord-104279-choywmwd	246	25	STE13	STE13	NNP
cord-104279-choywmwd	246	26	DNA	dna	NN
cord-104279-choywmwd	246	27	sequence	sequence	NN
cord-104279-choywmwd	246	28	,	,	,
cord-104279-choywmwd	246	29	107,200	107,200	CD
cord-104279-choywmwd	246	30	D	d	NN
cord-104279-choywmwd	246	31	(	(	-LRB-
cord-104279-choywmwd	246	32	C.	C.	NNP
cord-104279-choywmwd	246	33	A.	A.	NNP
cord-104279-choywmwd	246	34	Flanagan	Flanagan	NNP
cord-104279-choywmwd	246	35	,	,	,
cord-104279-choywmwd	246	36	D.	D.	NNP
cord-104279-choywmwd	246	37	A.	A.	NNP
cord-104279-choywmwd	246	38	Barnes	Barnes	NNP
cord-104279-choywmwd	246	39	,	,	,
cord-104279-choywmwd	246	40	M.	M.	NNP
cord-104279-choywmwd	246	41	C.	C.	NNP
cord-104279-choywmwd	246	42	Flessel	Flessel	NNP
cord-104279-choywmwd	246	43	,	,	,
cord-104279-choywmwd	247	1	andJ.	andJ.	NNP
cord-104279-choywmwd	247	2	Thorner	Thorner	NNP
cord-104279-choywmwd	247	3	,	,	,
cord-104279-choywmwd	247	4	manuscript	manuscript	NN
cord-104279-choywmwd	247	5	submired	submired	NNP
cord-104279-choywmwd	247	6	for	for	IN
cord-104279-choywmwd	247	7	publication	publication	NN
cord-104279-choywmwd	247	8	)	)	-RRB-
cord-104279-choywmwd	247	9	.	.	.
cord-104279-choywmwd	248	1	Indirect	indirect	JJ
cord-104279-choywmwd	248	2	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	248	3	microscopy	microscopy	NN
cord-104279-choywmwd	248	4	of	of	IN
cord-104279-choywmwd	248	5	cells	cell	NNS
cord-104279-choywmwd	248	6	containing	contain	VBG
cord-104279-choywmwd	248	7	the	the	DT
cord-104279-choywmwd	248	8	$	$	$
cord-104279-choywmwd	248	9	7F,/3	7f,/3	CD
cord-104279-choywmwd	248	10	gene	gene	NN
cord-104279-choywmwd	248	11	on	on	IN
cord-104279-choywmwd	248	12	a	a	DT
cord-104279-choywmwd	248	13	2	2	CD
cord-104279-choywmwd	248	14	#	#	NN
cord-104279-choywmwd	248	15	plasmid	plasmid	NN
cord-104279-choywmwd	248	16	showed	show	VBD
cord-104279-choywmwd	248	17	that	that	IN
cord-104279-choywmwd	248	18	DPAP	DPAP	NNP
cord-104279-choywmwd	248	19	A	A	NNP
cord-104279-choywmwd	248	20	was	be	VBD
cord-104279-choywmwd	248	21	localized	localize	VBN
cord-104279-choywmwd	248	22	to	to	IN
cord-104279-choywmwd	248	23	several	several	JJ
cord-104279-choywmwd	248	24	non	non	JJ
cord-104279-choywmwd	248	25	-	-	JJ
cord-104279-choywmwd	248	26	vacuolar	vacuolar	JJ
cord-104279-choywmwd	248	27	puncture	puncture	NN
cord-104279-choywmwd	248	28	patches	patch	NNS
cord-104279-choywmwd	248	29	dispersed	disperse	VBN
cord-104279-choywmwd	248	30	throughout	throughout	IN
cord-104279-choywmwd	248	31	the	the	DT
cord-104279-choywmwd	248	32	cell	cell	NN
cord-104279-choywmwd	248	33	,	,	,
cord-104279-choywmwd	248	34	as	as	IN
cord-104279-choywmwd	248	35	judged	judge	VBN
cord-104279-choywmwd	248	36	by	by	IN
cord-104279-choywmwd	248	37	Nomarski	Nomarski	NNP
cord-104279-choywmwd	248	38	optics	optic	NNS
cord-104279-choywmwd	248	39	and	and	CC
cord-104279-choywmwd	248	40	in	in	IN
cord-104279-choywmwd	248	41	double	double	JJ
cord-104279-choywmwd	248	42	staining	stain	VBG
cord-104279-choywmwd	248	43	experiments	experiment	NNS
cord-104279-choywmwd	248	44	with	with	IN
cord-104279-choywmwd	248	45	Vat2p	Vat2p	VBG
cord-104279-choywmwd	248	46	antibody	antibody	NN
cord-104279-choywmwd	248	47	(	(	-LRB-
cord-104279-choywmwd	248	48	Fig	Fig	NNP
cord-104279-choywmwd	248	49	.	.	NNP
cord-104279-choywmwd	248	50	7	7	CD
cord-104279-choywmwd	248	51	,	,	,
cord-104279-choywmwd	248	52	D	D	NNP
cord-104279-choywmwd	248	53	-	-	HYPH
cord-104279-choywmwd	248	54	U	U	NNP
cord-104279-choywmwd	248	55	)	)	-RRB-
cord-104279-choywmwd	248	56	.	.	.
cord-104279-choywmwd	249	1	This	this	DT
cord-104279-choywmwd	249	2	signal	signal	NN
cord-104279-choywmwd	249	3	was	be	VBD
cord-104279-choywmwd	249	4	absent	absent	JJ
cord-104279-choywmwd	249	5	in	in	IN
cord-104279-choywmwd	249	6	a	a	DT
cord-104279-choywmwd	249	7	steI3A	steI3A	``
cord-104279-choywmwd	249	8	strain	strain	NN
cord-104279-choywmwd	249	9	(	(	-LRB-
cord-104279-choywmwd	249	10	Fig	Fig	NNP
cord-104279-choywmwd	249	11	.	.	NNP
cord-104279-choywmwd	249	12	7	7	CD
cord-104279-choywmwd	249	13	,	,	,
cord-104279-choywmwd	249	14	A	A	NNP
cord-104279-choywmwd	249	15	-	-	HYPH
cord-104279-choywmwd	249	16	C	C	NNP
cord-104279-choywmwd	249	17	)	)	-RRB-
cord-104279-choywmwd	249	18	.	.	.
cord-104279-choywmwd	250	1	This	this	DT
cord-104279-choywmwd	250	2	staining	staining	NN
cord-104279-choywmwd	250	3	pattern	pattern	NN
cord-104279-choywmwd	250	4	is	be	VBZ
cord-104279-choywmwd	250	5	typical	typical	JJ
cord-104279-choywmwd	250	6	of	of	IN
cord-104279-choywmwd	250	7	the	the	DT
cord-104279-choywmwd	250	8	Golgi	Golgi	NNP
cord-104279-choywmwd	250	9	apparatus	apparatus	NN
cord-104279-choywmwd	250	10	,	,	,
cord-104279-choywmwd	250	11	as	as	IN
cord-104279-choywmwd	250	12	determined	determine	VBN
cord-104279-choywmwd	250	13	by	by	IN
cord-104279-choywmwd	250	14	the	the	DT
cord-104279-choywmwd	250	15	localization	localization	NN
cord-104279-choywmwd	250	16	of	of	IN
cord-104279-choywmwd	250	17	Kex2p	Kex2p	NNP
cord-104279-choywmwd	250	18	and	and	CC
cord-104279-choywmwd	250	19	Kexlp	Kexlp	NNP
cord-104279-choywmwd	250	20	(	(	-LRB-
cord-104279-choywmwd	250	21	A	A	NNP
cord-104279-choywmwd	250	22	,	,	,
cord-104279-choywmwd	250	23	Cooper	Cooper	NNP
cord-104279-choywmwd	250	24	and	and	CC
cord-104279-choywmwd	250	25	H.	H.	NNP
cord-104279-choywmwd	250	26	Bussey	Bussey	NNP
cord-104279-choywmwd	250	27	,	,	,
cord-104279-choywmwd	250	28	manuscript	manuscript	NN
cord-104279-choywmwd	250	29	submitted	submit	VBN
cord-104279-choywmwd	250	30	for	for	IN
cord-104279-choywmwd	250	31	publication	publication	NN
cord-104279-choywmwd	250	32	)	)	-RRB-
cord-104279-choywmwd	250	33	.	.	.
cord-104279-choywmwd	251	1	Attempts	attempt	NNS
cord-104279-choywmwd	251	2	to	to	TO
cord-104279-choywmwd	251	3	localize	localize	VB
cord-104279-choywmwd	251	4	DPAP	DPAP	NNP
cord-104279-choywmwd	251	5	A	A	NNP
cord-104279-choywmwd	251	6	in	in	IN
cord-104279-choywmwd	251	7	cells	cell	NNS
cord-104279-choywmwd	251	8	containing	contain	VBG
cord-104279-choywmwd	251	9	only	only	RB
cord-104279-choywmwd	251	10	the	the	DT
cord-104279-choywmwd	251	11	chromosomal	chromosomal	JJ
cord-104279-choywmwd	251	12	copy	copy	NN
cord-104279-choywmwd	251	13	of	of	IN
cord-104279-choywmwd	251	14	STE/3	STE/3	NNP
cord-104279-choywmwd	251	15	were	be	VBD
cord-104279-choywmwd	251	16	unsuccessful	unsuccessful	JJ
cord-104279-choywmwd	251	17	due	due	IN
cord-104279-choywmwd	251	18	to	to	IN
cord-104279-choywmwd	251	19	the	the	DT
cord-104279-choywmwd	251	20	low	low	JJ
cord-104279-choywmwd	251	21	abundance	abundance	NN
cord-104279-choywmwd	251	22	of	of	IN
cord-104279-choywmwd	251	23	the	the	DT
cord-104279-choywmwd	251	24	protein	protein	NN
cord-104279-choywmwd	251	25	.	.	.
cord-104279-choywmwd	252	1	Because	because	IN
cord-104279-choywmwd	252	2	the	the	DT
cord-104279-choywmwd	252	3	copy	copy	NN
cord-104279-choywmwd	252	4	number	number	NN
cord-104279-choywmwd	252	5	of	of	IN
cord-104279-choywmwd	252	6	yeast	yeast	NN
cord-104279-choywmwd	252	7	2/~	2/~	CD
cord-104279-choywmwd	252	8	plasmids	plasmid	NNS
cord-104279-choywmwd	252	9	can	can	MD
cord-104279-choywmwd	252	10	vary	vary	VB
cord-104279-choywmwd	252	11	from	from	IN
cord-104279-choywmwd	252	12	10	10	CD
cord-104279-choywmwd	252	13	-	-	SYM
cord-104279-choywmwd	252	14	40	40	CD
cord-104279-choywmwd	252	15	copies	copy	NNS
cord-104279-choywmwd	252	16	/	/	SYM
cord-104279-choywmwd	252	17	cell	cell	NN
cord-104279-choywmwd	252	18	within	within	IN
cord-104279-choywmwd	252	19	a	a	DT
cord-104279-choywmwd	252	20	population	population	NN
cord-104279-choywmwd	252	21	(	(	-LRB-
cord-104279-choywmwd	252	22	Rose	Rose	NNP
cord-104279-choywmwd	252	23	and	and	CC
cord-104279-choywmwd	252	24	Broach	Broach	NNP
cord-104279-choywmwd	252	25	,	,	,
cord-104279-choywmwd	252	26	1991	1991	CD
cord-104279-choywmwd	252	27	)	)	-RRB-
cord-104279-choywmwd	252	28	,	,	,
cord-104279-choywmwd	252	29	the	the	DT
cord-104279-choywmwd	252	30	fluorescence	fluorescence	NN
cord-104279-choywmwd	252	31	signal	signal	NN
cord-104279-choywmwd	252	32	corresponding	correspond	VBG
cord-104279-choywmwd	252	33	to	to	IN
cord-104279-choywmwd	252	34	DPAP	DPAP	NNP
cord-104279-choywmwd	252	35	A	A	NNP
cord-104279-choywmwd	252	36	when	when	WRB
cord-104279-choywmwd	252	37	expressed	express	VBN
cord-104279-choywmwd	252	38	from	from	IN
cord-104279-choywmwd	252	39	a	a	DT
cord-104279-choywmwd	252	40	2	2	CD
cord-104279-choywmwd	252	41	#	#	NN
cord-104279-choywmwd	252	42	plasmid	plasmid	NN
cord-104279-choywmwd	252	43	also	also	RB
cord-104279-choywmwd	252	44	varied	varied	JJ
cord-104279-choywmwd	252	45	from	from	IN
cord-104279-choywmwd	252	46	cell	cell	NN
cord-104279-choywmwd	252	47	to	to	IN
cord-104279-choywmwd	252	48	cell	cell	NN
cord-104279-choywmwd	252	49	,	,	,
cord-104279-choywmwd	252	50	ranging	range	VBG
cord-104279-choywmwd	252	51	from	from	IN
cord-104279-choywmwd	252	52	a	a	DT
cord-104279-choywmwd	252	53	weak	weak	JJ
cord-104279-choywmwd	252	54	signal	signal	NN
cord-104279-choywmwd	252	55	to	to	IN
cord-104279-choywmwd	252	56	a	a	DT
cord-104279-choywmwd	252	57	very	very	RB
cord-104279-choywmwd	252	58	strong	strong	JJ
cord-104279-choywmwd	252	59	signal	signal	NN
cord-104279-choywmwd	252	60	,	,	,
cord-104279-choywmwd	252	61	whereas	whereas	IN
cord-104279-choywmwd	252	62	the	the	DT
cord-104279-choywmwd	252	63	intensity	intensity	NN
cord-104279-choywmwd	252	64	of	of	IN
cord-104279-choywmwd	252	65	the	the	DT
cord-104279-choywmwd	252	66	Vat2p	Vat2p	NNP
cord-104279-choywmwd	252	67	signal	signal	NN
cord-104279-choywmwd	252	68	was	be	VBD
cord-104279-choywmwd	252	69	consistent	consistent	JJ
cord-104279-choywmwd	252	70	from	from	IN
cord-104279-choywmwd	252	71	cell	cell	NN
cord-104279-choywmwd	252	72	to	to	IN
cord-104279-choywmwd	252	73	cell	cell	NN
cord-104279-choywmwd	252	74	.	.	.
cord-104279-choywmwd	253	1	All	all	DT
cord-104279-choywmwd	253	2	of	of	IN
cord-104279-choywmwd	253	3	the	the	DT
cord-104279-choywmwd	253	4	cells	cell	NNS
cord-104279-choywmwd	253	5	that	that	WDT
cord-104279-choywmwd	253	6	showed	show	VBD
cord-104279-choywmwd	253	7	a	a	DT
cord-104279-choywmwd	253	8	signal	signal	NN
cord-104279-choywmwd	253	9	with	with	IN
cord-104279-choywmwd	253	10	the	the	DT
cord-104279-choywmwd	253	11	DPAP	DPAP	NNP
cord-104279-choywmwd	253	12	A	a	DT
cord-104279-choywmwd	253	13	antibody	antibody	NN
cord-104279-choywmwd	253	14	displayed	display	VBD
cord-104279-choywmwd	253	15	a	a	DT
cord-104279-choywmwd	253	16	Golgi	golgi	NN
cord-104279-choywmwd	253	17	-	-	HYPH
cord-104279-choywmwd	253	18	staining	staining	NN
cord-104279-choywmwd	253	19	pattern	pattern	NN
cord-104279-choywmwd	253	20	(	(	-LRB-
cord-104279-choywmwd	253	21	Fig	Fig	NNP
cord-104279-choywmwd	253	22	.	.	NNP
cord-104279-choywmwd	253	23	7	7	CD
cord-104279-choywmwd	253	24	,	,	,
cord-104279-choywmwd	253	25	D	D	NNP
cord-104279-choywmwd	253	26	-	-	HYPH
cord-104279-choywmwd	253	27	U	U	NNP
cord-104279-choywmwd	253	28	;	;	:
cord-104279-choywmwd	253	29	Table	table	NN
cord-104279-choywmwd	253	30	 	 	_SP
cord-104279-choywmwd	253	31	I	i	NN
cord-104279-choywmwd	253	32	)	)	-RRB-
cord-104279-choywmwd	253	33	;	;	:
cord-104279-choywmwd	253	34	however	however	RB
cord-104279-choywmwd	253	35	,	,	,
cord-104279-choywmwd	253	36	cells	cell	NNS
cord-104279-choywmwd	253	37	exhibiting	exhibit	VBG
cord-104279-choywmwd	253	38	a	a	DT
cord-104279-choywmwd	253	39	very	very	RB
cord-104279-choywmwd	253	40	strong	strong	JJ
cord-104279-choywmwd	253	41	signal	signal	NN
cord-104279-choywmwd	253	42	,	,	,
cord-104279-choywmwd	253	43	presumably	presumably	RB
cord-104279-choywmwd	253	44	due	due	IN
cord-104279-choywmwd	253	45	to	to	IN
cord-104279-choywmwd	253	46	a	a	DT
cord-104279-choywmwd	253	47	high	high	JJ
cord-104279-choywmwd	253	48	dosage	dosage	NN
cord-104279-choywmwd	253	49	of	of	IN
cord-104279-choywmwd	253	50	the	the	DT
cord-104279-choywmwd	253	51	STE/3	STE/3	NNP
cord-104279-choywmwd	253	52	gene	gene	NN
cord-104279-choywmwd	253	53	,	,	,
cord-104279-choywmwd	253	54	also	also	RB
cord-104279-choywmwd	253	55	showed	show	VBD
cord-104279-choywmwd	253	56	vacuolar	vacuolar	JJ
cord-104279-choywmwd	253	57	staining	staining	NN
cord-104279-choywmwd	253	58	(	(	-LRB-
cord-104279-choywmwd	253	59	5	5	CD
cord-104279-choywmwd	253	60	%	%	NN
cord-104279-choywmwd	253	61	of	of	IN
cord-104279-choywmwd	253	62	the	the	DT
cord-104279-choywmwd	253	63	cells	cell	NNS
cord-104279-choywmwd	253	64	ofapep4	ofapep4	NN
cord-104279-choywmwd	253	65	-	-	HYPH
cord-104279-choywmwd	253	66	3	3	CD
cord-104279-choywmwd	253	67	strain	strain	NN
cord-104279-choywmwd	253	68	;	;	:
cord-104279-choywmwd	253	69	Table	table	NN
cord-104279-choywmwd	253	70	I	i	CD
cord-104279-choywmwd	253	71	)	)	-RRB-
cord-104279-choywmwd	253	72	.	.	.
cord-104279-choywmwd	254	1	An	an	DT
cord-104279-choywmwd	254	2	example	example	NN
cord-104279-choywmwd	254	3	of	of	IN
cord-104279-choywmwd	254	4	this	this	DT
cord-104279-choywmwd	254	5	is	be	VBZ
cord-104279-choywmwd	254	6	shown	show	VBN
cord-104279-choywmwd	254	7	in	in	IN
cord-104279-choywmwd	254	8	Fig	Fig	NNP
cord-104279-choywmwd	254	9	.	.	.
cord-104279-choywmwd	255	1	7	7	CD
cord-104279-choywmwd	255	2	,	,	,
cord-104279-choywmwd	255	3	(	(	-LRB-
cord-104279-choywmwd	255	4	~	~	NFP
cord-104279-choywmwd	255	5	S	S	NNP
cord-104279-choywmwd	255	6	,	,	,
cord-104279-choywmwd	255	7	and	and	CC
cord-104279-choywmwd	255	8	U	U	NNP
cord-104279-choywmwd	255	9	)	)	-RRB-
cord-104279-choywmwd	255	10	.	.	.
cord-104279-choywmwd	256	1	A	a	DT
cord-104279-choywmwd	256	2	single	single	JJ
cord-104279-choywmwd	256	3	cell	cell	NN
cord-104279-choywmwd	256	4	exhibiting	exhibit	VBG
cord-104279-choywmwd	256	5	a	a	DT
cord-104279-choywmwd	256	6	strong	strong	JJ
cord-104279-choywmwd	256	7	DPAP	DPAP	NNP
cord-104279-choywmwd	256	8	A	a	DT
cord-104279-choywmwd	256	9	signal	signal	NN
cord-104279-choywmwd	256	10	is	be	VBZ
cord-104279-choywmwd	256	11	shown	show	VBN
cord-104279-choywmwd	256	12	,	,	,
cord-104279-choywmwd	256	13	and	and	CC
cord-104279-choywmwd	256	14	the	the	DT
cord-104279-choywmwd	256	15	signal	signal	NN
cord-104279-choywmwd	256	16	stains	stain	NNS
cord-104279-choywmwd	256	17	both	both	CC
cord-104279-choywmwd	256	18	vacuolar	vacuolar	NNP
cord-104279-choywmwd	256	19	and	and	CC
cord-104279-choywmwd	256	20	punctate	punctate	NNP
cord-104279-choywmwd	256	21	non	non	JJ
cord-104279-choywmwd	256	22	-	-	JJ
cord-104279-choywmwd	256	23	vacuolar	vacuolar	JJ
cord-104279-choywmwd	256	24	structures	structure	NNS
cord-104279-choywmwd	256	25	,	,	,
cord-104279-choywmwd	256	26	as	as	IN
cord-104279-choywmwd	256	27	judged	judge	VBN
cord-104279-choywmwd	256	28	by	by	IN
cord-104279-choywmwd	256	29	co	co	NN
cord-104279-choywmwd	256	30	-	-	NN
cord-104279-choywmwd	256	31	localization	localization	NN
cord-104279-choywmwd	256	32	with	with	IN
cord-104279-choywmwd	256	33	Vat2p	Vat2p	NNP
cord-104279-choywmwd	256	34	.	.	.
cord-104279-choywmwd	257	1	The	the	DT
cord-104279-choywmwd	257	2	percentage	percentage	NN
cord-104279-choywmwd	257	3	of	of	IN
cord-104279-choywmwd	257	4	cells	cell	NNS
cord-104279-choywmwd	257	5	showing	show	VBG
cord-104279-choywmwd	257	6	vacuolar	vacuolar	JJ
cord-104279-choywmwd	257	7	localization	localization	NN
cord-104279-choywmwd	257	8	of	of	IN
cord-104279-choywmwd	257	9	DPAP	DPAP	NNP
cord-104279-choywmwd	257	10	A	A	NNP
cord-104279-choywmwd	257	11	decreased	decrease	VBD
cord-104279-choywmwd	257	12	significantly	significantly	RB
cord-104279-choywmwd	257	13	in	in	IN
cord-104279-choywmwd	257	14	an	an	DT
cord-104279-choywmwd	257	15	otherwise	otherwise	RB
cord-104279-choywmwd	257	16	isogenic	isogenic	JJ
cord-104279-choywmwd	257	17	PEP4	PEP4	NNP
cord-104279-choywmwd	257	18	strain	strain	NN
cord-104279-choywmwd	257	19	,	,	,
cord-104279-choywmwd	257	20	which	which	WDT
cord-104279-choywmwd	257	21	contains	contain	VBZ
cord-104279-choywmwd	257	22	the	the	DT
cord-104279-choywmwd	257	23	full	full	JJ
cord-104279-choywmwd	257	24	complement	complement	NN
cord-104279-choywmwd	257	25	of	of	IN
cord-104279-choywmwd	257	26	vacuolar	vacuolar	JJ
cord-104279-choywmwd	257	27	proteases	protease	NNS
cord-104279-choywmwd	257	28	(	(	-LRB-
cord-104279-choywmwd	257	29	I	-PRON-	PRP
cord-104279-choywmwd	257	30	%	%	NN
cord-104279-choywmwd	257	31	;	;	:
cord-104279-choywmwd	257	32	Table	table	NN
cord-104279-choywmwd	257	33	I	i	CD
cord-104279-choywmwd	257	34	)	)	-RRB-
cord-104279-choywmwd	257	35	.	.	.
cord-104279-choywmwd	258	1	Thus	thus	RB
cord-104279-choywmwd	258	2	,	,	,
cord-104279-choywmwd	258	3	cells	cell	NNS
cord-104279-choywmwd	258	4	producing	produce	VBG
cord-104279-choywmwd	258	5	high	high	JJ
cord-104279-choywmwd	258	6	levels	level	NNS
cord-104279-choywmwd	258	7	of	of	IN
cord-104279-choywmwd	258	8	DPAP	DPAP	NNP
cord-104279-choywmwd	258	9	A	A	NNP
cord-104279-choywmwd	258	10	showed	show	VBD
cord-104279-choywmwd	258	11	mislocalization	mislocalization	NN
cord-104279-choywmwd	258	12	of	of	IN
cord-104279-choywmwd	258	13	the	the	DT
cord-104279-choywmwd	258	14	protein	protein	NN
cord-104279-choywmwd	258	15	to	to	IN
cord-104279-choywmwd	258	16	the	the	DT
cord-104279-choywmwd	258	17	vacuolar	vacuolar	NNP
cord-104279-choywmwd	258	18	membrane	membrane	NN
cord-104279-choywmwd	258	19	,	,	,
cord-104279-choywmwd	258	20	with	with	IN
cord-104279-choywmwd	258	21	the	the	DT
cord-104279-choywmwd	258	22	mislocalized	mislocalize	VBN
cord-104279-choywmwd	258	23	protein	protein	NN
cord-104279-choywmwd	258	24	degraded	degrade	VBN
cord-104279-choywmwd	258	25	in	in	IN
cord-104279-choywmwd	258	26	a	a	DT
cord-104279-choywmwd	258	27	vacuolar	vacuolar	JJ
cord-104279-choywmwd	258	28	proteasedependent	proteasedependent	NN
cord-104279-choywmwd	258	29	fashion	fashion	NN
cord-104279-choywmwd	258	30	.	.	.
cord-104279-choywmwd	259	1	DPAP	DPAP	NNP
cord-104279-choywmwd	259	2	enzyme	enzyme	NN
cord-104279-choywmwd	259	3	assays	assay	NNS
cord-104279-choywmwd	259	4	on	on	IN
cord-104279-choywmwd	259	5	permeabilized	permeabilized	JJ
cord-104279-choywmwd	259	6	and	and	CC
cord-104279-choywmwd	259	7	non	non	JJ
cord-104279-choywmwd	259	8	-	-	JJ
cord-104279-choywmwd	259	9	permeabilized	permeabilized	JJ
cord-104279-choywmwd	259	10	cells	cell	NNS
cord-104279-choywmwd	259	11	expressing	express	VBG
cord-104279-choywmwd	259	12	DPAP	DPAP	NNP
cord-104279-choywmwd	259	13	A	A	NNP
cord-104279-choywmwd	259	14	from	from	IN
cord-104279-choywmwd	259	15	either	either	CC
cord-104279-choywmwd	259	16	CEN	CEN	NNP
cord-104279-choywmwd	259	17	or	or	CC
cord-104279-choywmwd	259	18	2	2	CD
cord-104279-choywmwd	259	19	g	g	NN
cord-104279-choywmwd	259	20	plasmids	plasmid	NNS
cord-104279-choywmwd	259	21	showed	show	VBD
cord-104279-choywmwd	259	22	that	that	IN
cord-104279-choywmwd	259	23	,	,	,
cord-104279-choywmwd	259	24	088	088	CD
cord-104279-choywmwd	259	25	%	%	NN
cord-104279-choywmwd	259	26	of	of	IN
cord-104279-choywmwd	259	27	the	the	DT
cord-104279-choywmwd	259	28	total	total	JJ
cord-104279-choywmwd	259	29	activity	activity	NN
cord-104279-choywmwd	259	30	was	be	VBD
cord-104279-choywmwd	259	31	intracellular	intracellular	JJ
cord-104279-choywmwd	259	32	;	;	:
cord-104279-choywmwd	259	33	thus	thus	RB
cord-104279-choywmwd	259	34	,	,	,
cord-104279-choywmwd	259	35	it	-PRON-	PRP
cord-104279-choywmwd	259	36	is	be	VBZ
cord-104279-choywmwd	259	37	possible	possible	JJ
cord-104279-choywmwd	259	38	that	that	IN
cord-104279-choywmwd	259	39	a	a	DT
cord-104279-choywmwd	259	40	small	small	JJ
cord-104279-choywmwd	259	41	percentage	percentage	NN
cord-104279-choywmwd	259	42	of	of	IN
cord-104279-choywmwd	259	43	DPAP	DPAP	NNP
cord-104279-choywmwd	259	44	A	A	NNP
cord-104279-choywmwd	259	45	is	be	VBZ
cord-104279-choywmwd	259	46	present	present	JJ
cord-104279-choywmwd	259	47	at	at	IN
cord-104279-choywmwd	259	48	the	the	DT
cord-104279-choywmwd	259	49	cell	cell	NN
cord-104279-choywmwd	259	50	surface	surface	NN
cord-104279-choywmwd	259	51	,	,	,
cord-104279-choywmwd	259	52	even	even	RB
cord-104279-choywmwd	259	53	though	though	IN
cord-104279-choywmwd	259	54	no	no	DT
cord-104279-choywmwd	259	55	plasma	plasma	NN
cord-104279-choywmwd	259	56	membrane	membrane	NN
cord-104279-choywmwd	259	57	staining	staining	NN
cord-104279-choywmwd	259	58	was	be	VBD
cord-104279-choywmwd	259	59	detected	detect	VBN
cord-104279-choywmwd	259	60	by	by	IN
cord-104279-choywmwd	259	61	indirect	indirect	JJ
cord-104279-choywmwd	259	62	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	259	63	,	,	,
cord-104279-choywmwd	260	1	However	however	RB
cord-104279-choywmwd	260	2	,	,	,
cord-104279-choywmwd	260	3	the	the	DT
cord-104279-choywmwd	260	4	apparent	apparent	JJ
cord-104279-choywmwd	260	5	extraceUular	extraceuular	NN
cord-104279-choywmwd	261	1	DPAP	DPAP	NNP
cord-104279-choywmwd	261	2	A	a	DT
cord-104279-choywmwd	261	3	enzyme	enzyme	NN
cord-104279-choywmwd	261	4	activity	activity	NN
cord-104279-choywmwd	261	5	could	could	MD
cord-104279-choywmwd	261	6	in	in	IN
cord-104279-choywmwd	261	7	part	part	NN
cord-104279-choywmwd	261	8	be	be	VB
cord-104279-choywmwd	261	9	due	due	JJ
cord-104279-choywmwd	261	10	to	to	IN
cord-104279-choywmwd	261	11	a	a	DT
cord-104279-choywmwd	261	12	small	small	JJ
cord-104279-choywmwd	261	13	degree	degree	NN
cord-104279-choywmwd	261	14	of	of	IN
cord-104279-choywmwd	261	15	cell	cell	NN
cord-104279-choywmwd	261	16	lysis	lysis	NN
cord-104279-choywmwd	261	17	during	during	IN
cord-104279-choywmwd	261	18	the	the	DT
cord-104279-choywmwd	261	19	assay	assay	NN
cord-104279-choywmwd	261	20	.	.	.
cord-104279-choywmwd	262	1	To	to	TO
cord-104279-choywmwd	262	2	determine	determine	VB
cord-104279-choywmwd	262	3	whether	whether	IN
cord-104279-choywmwd	262	4	a	a	DT
cord-104279-choywmwd	262	5	specific	specific	JJ
cord-104279-choywmwd	262	6	domain	domain	NN
cord-104279-choywmwd	262	7	of	of	IN
cord-104279-choywmwd	262	8	DPAP	DPAP	NNP
cord-104279-choywmwd	262	9	A	a	DT
cord-104279-choywmwd	262	10	con	con	NN
cord-104279-choywmwd	262	11	-	-	:
cord-104279-choywmwd	262	12	The	the	DT
cord-104279-choywmwd	262	13	Journal	Journal	NNP
cord-104279-choywmwd	262	14	of	of	IN
cord-104279-choywmwd	262	15	Cell	Cell	NNP
cord-104279-choywmwd	262	16	Biology	Biology	NNP
cord-104279-choywmwd	262	17	,	,	,
cord-104279-choywmwd	262	18	Volume	volume	NN
cord-104279-choywmwd	262	19	119	119	CD
cord-104279-choywmwd	262	20	,	,	,
cord-104279-choywmwd	262	21	1992	1992	CD
cord-104279-choywmwd	262	22	tained	taine	VBD
cord-104279-choywmwd	262	23	the	the	DT
cord-104279-choywmwd	262	24	signal	signal	NN
cord-104279-choywmwd	262	25	for	for	IN
cord-104279-choywmwd	262	26	Golgi	Golgi	NNP
cord-104279-choywmwd	262	27	retention	retention	NN
cord-104279-choywmwd	262	28	,	,	,
cord-104279-choywmwd	262	29	the	the	DT
cord-104279-choywmwd	262	30	fusion	fusion	NN
cord-104279-choywmwd	262	31	proteins	protein	NNS
cord-104279-choywmwd	262	32	AA	AA	NNP
cord-104279-choywmwd	262	33	-	-	HYPH
cord-104279-choywmwd	262	34	B	B	NNP
cord-104279-choywmwd	262	35	and	and	CC
cord-104279-choywmwd	262	36	A	A	NNP
cord-104279-choywmwd	262	37	-	-	HYPH
cord-104279-choywmwd	262	38	BB	BB	NNP
cord-104279-choywmwd	262	39	were	be	VBD
cord-104279-choywmwd	262	40	constructed	construct	VBN
cord-104279-choywmwd	262	41	(	(	-LRB-
cord-104279-choywmwd	262	42	Fig	Fig	NNP
cord-104279-choywmwd	262	43	.	.	.
cord-104279-choywmwd	262	44	1	1	CD
cord-104279-choywmwd	262	45	C	C	NNP
cord-104279-choywmwd	262	46	)	)	-RRB-
cord-104279-choywmwd	262	47	,	,	,
cord-104279-choywmwd	262	48	in	in	IN
cord-104279-choywmwd	262	49	which	which	WDT
cord-104279-choywmwd	262	50	the	the	DT
cord-104279-choywmwd	262	51	lumenal	lumenal	JJ
cord-104279-choywmwd	262	52	domain	domain	NN
cord-104279-choywmwd	262	53	of	of	IN
cord-104279-choywmwd	262	54	DPAP	DPAP	NNP
cord-104279-choywmwd	262	55	A	A	NNP
cord-104279-choywmwd	262	56	was	be	VBD
cord-104279-choywmwd	262	57	exchanged	exchange	VBN
cord-104279-choywmwd	262	58	for	for	IN
cord-104279-choywmwd	262	59	that	that	DT
cord-104279-choywmwd	262	60	of	of	IN
cord-104279-choywmwd	262	61	DPAP	DPAP	NNP
cord-104279-choywmwd	262	62	B	B	NNP
cord-104279-choywmwd	262	63	(	(	-LRB-
cord-104279-choywmwd	262	64	AA	AA	NNP
cord-104279-choywmwd	262	65	-	-	HYPH
cord-104279-choywmwd	262	66	B	B	NNP
cord-104279-choywmwd	262	67	)	)	-RRB-
cord-104279-choywmwd	262	68	,	,	,
cord-104279-choywmwd	262	69	and	and	CC
cord-104279-choywmwd	262	70	the	the	DT
cord-104279-choywmwd	262	71	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	262	72	domain	domain	NN
cord-104279-choywmwd	262	73	of	of	IN
cord-104279-choywmwd	262	74	DPAP	DPAP	NNP
cord-104279-choywmwd	262	75	B	B	NNP
cord-104279-choywmwd	262	76	was	be	VBD
cord-104279-choywmwd	262	77	exchanged	exchange	VBN
cord-104279-choywmwd	262	78	for	for	IN
cord-104279-choywmwd	262	79	that	that	DT
cord-104279-choywmwd	262	80	of	of	IN
cord-104279-choywmwd	262	81	DPAP	DPAP	NNP
cord-104279-choywmwd	262	82	A	A	NNP
cord-104279-choywmwd	262	83	(	(	-LRB-
cord-104279-choywmwd	262	84	A	A	NNP
cord-104279-choywmwd	262	85	-	-	HYPH
cord-104279-choywmwd	262	86	BB	bb	NN
cord-104279-choywmwd	262	87	)	)	-RRB-
cord-104279-choywmwd	262	88	.	.	.
cord-104279-choywmwd	263	1	Immunoprecipitation	Immunoprecipitation	NNP
cord-104279-choywmwd	263	2	of	of	IN
cord-104279-choywmwd	263	3	AA	AA	NNP
cord-104279-choywmwd	263	4	-	-	HYPH
cord-104279-choywmwd	263	5	B	B	NNP
cord-104279-choywmwd	263	6	and	and	CC
cord-104279-choywmwd	263	7	A	A	NNP
cord-104279-choywmwd	263	8	-	-	HYPH
cord-104279-choywmwd	263	9	BB	bb	NN
cord-104279-choywmwd	263	10	from	from	IN
cord-104279-choywmwd	263	11	35S	35S	NNP
cord-104279-choywmwd	263	12	-	-	HYPH
cord-104279-choywmwd	263	13	labeled	label	VBN
cord-104279-choywmwd	263	14	cells	cell	NNS
cord-104279-choywmwd	263	15	,	,	,
cord-104279-choywmwd	263	16	followed	follow	VBN
cord-104279-choywmwd	263	17	by	by	IN
cord-104279-choywmwd	263	18	SDS	SDS	NNP
cord-104279-choywmwd	263	19	-	-	HYPH
cord-104279-choywmwd	263	20	PAGE	PAGE	NNP
cord-104279-choywmwd	263	21	and	and	CC
cord-104279-choywmwd	263	22	fluorography	fluorography	NNP
cord-104279-choywmwd	263	23	,	,	,
cord-104279-choywmwd	263	24	showed	show	VBD
cord-104279-choywmwd	263	25	that	that	IN
cord-104279-choywmwd	263	26	both	both	DT
cord-104279-choywmwd	263	27	fusion	fusion	NN
cord-104279-choywmwd	263	28	proteins	protein	NNS
cord-104279-choywmwd	263	29	were	be	VBD
cord-104279-choywmwd	263	30	glycosylated	glycosylate	VBN
cord-104279-choywmwd	263	31	and	and	CC
cord-104279-choywmwd	263	32	treatment	treatment	NN
cord-104279-choywmwd	263	33	of	of	IN
cord-104279-choywmwd	263	34	the	the	DT
cord-104279-choywmwd	263	35	immunoprecipitates	immunoprecipitate	NNS
cord-104279-choywmwd	263	36	with	with	IN
cord-104279-choywmwd	263	37	Endo	Endo	NNP
cord-104279-choywmwd	263	38	H	H	NNP
cord-104279-choywmwd	263	39	showed	show	VBD
cord-104279-choywmwd	263	40	that	that	IN
cord-104279-choywmwd	263	41	the	the	DT
cord-104279-choywmwd	263	42	proteins	protein	NNS
cord-104279-choywmwd	263	43	were	be	VBD
cord-104279-choywmwd	263	44	of	of	IN
cord-104279-choywmwd	263	45	the	the	DT
cord-104279-choywmwd	263	46	expected	expect	VBN
cord-104279-choywmwd	263	47	size	size	NN
cord-104279-choywmwd	263	48	(	(	-LRB-
cord-104279-choywmwd	263	49	Fig	Fig	NNP
cord-104279-choywmwd	263	50	.	.	.
cord-104279-choywmwd	263	51	6	6	CD
cord-104279-choywmwd	263	52	B	b	NN
cord-104279-choywmwd	263	53	)	)	-RRB-
cord-104279-choywmwd	263	54	.	.	.
cord-104279-choywmwd	264	1	The	the	DT
cord-104279-choywmwd	264	2	broadly	broadly	RB
cord-104279-choywmwd	264	3	migrating	migrate	VBG
cord-104279-choywmwd	264	4	species	specie	NNS
cord-104279-choywmwd	264	5	observed	observe	VBN
cord-104279-choywmwd	264	6	for	for	IN
cord-104279-choywmwd	264	7	AA	AA	NNP
cord-104279-choywmwd	264	8	-	-	HYPH
cord-104279-choywmwd	264	9	B	B	NNP
cord-104279-choywmwd	264	10	and	and	CC
cord-104279-choywmwd	264	11	A	A	NNP
cord-104279-choywmwd	264	12	-	-	HYPH
cord-104279-choywmwd	264	13	BB	bb	NN
cord-104279-choywmwd	264	14	indicate	indicate	VBP
cord-104279-choywmwd	264	15	that	that	IN
cord-104279-choywmwd	264	16	the	the	DT
cord-104279-choywmwd	264	17	proteins	protein	NNS
cord-104279-choywmwd	264	18	are	be	VBP
cord-104279-choywmwd	264	19	more	more	RBR
cord-104279-choywmwd	264	20	extensively	extensively	RB
cord-104279-choywmwd	264	21	modified	modify	VBN
cord-104279-choywmwd	264	22	in	in	IN
cord-104279-choywmwd	264	23	the	the	DT
cord-104279-choywmwd	264	24	Golgi	Golgi	NNP
cord-104279-choywmwd	264	25	apparatus	apparatus	NN
cord-104279-choywmwd	264	26	than	than	IN
cord-104279-choywmwd	264	27	DPAP	DPAP	NNP
cord-104279-choywmwd	264	28	A.	a.	NN
cord-104279-choywmwd	264	29	DPAP	dpap	NN
cord-104279-choywmwd	264	30	activity	activity	NN
cord-104279-choywmwd	264	31	assays	assay	NNS
cord-104279-choywmwd	264	32	on	on	IN
cord-104279-choywmwd	264	33	permeabilized	permeabilized	JJ
cord-104279-choywmwd	264	34	or	or	CC
cord-104279-choywmwd	264	35	non	non	JJ
cord-104279-choywmwd	264	36	-	-	JJ
cord-104279-choywmwd	264	37	permeabilized	permeabilized	JJ
cord-104279-choywmwd	264	38	whole	whole	JJ
cord-104279-choywmwd	264	39	cells	cell	NNS
cord-104279-choywmwd	265	1	demonstrated	demonstrate	VBD
cord-104279-choywmwd	265	2	that	that	IN
cord-104279-choywmwd	265	3	the	the	DT
cord-104279-choywmwd	265	4	AA	AA	NNP
cord-104279-choywmwd	265	5	-	-	HYPH
cord-104279-choywmwd	265	6	B	B	NNP
cord-104279-choywmwd	265	7	and	and	CC
cord-104279-choywmwd	265	8	A	a	DT
cord-104279-choywmwd	265	9	-	-	HYPH
cord-104279-choywmwd	265	10	BB	bb	NN
cord-104279-choywmwd	265	11	proteins	protein	NNS
cord-104279-choywmwd	265	12	were	be	VBD
cord-104279-choywmwd	265	13	enzymatically	enzymatically	RB
cord-104279-choywmwd	265	14	active	active	JJ
cord-104279-choywmwd	265	15	,	,	,
cord-104279-choywmwd	265	16	and	and	CC
cord-104279-choywmwd	265	17	that	that	IN
cord-104279-choywmwd	265	18	~80	~80	NFP
cord-104279-choywmwd	265	19	%	%	NN
cord-104279-choywmwd	265	20	of	of	IN
cord-104279-choywmwd	265	21	the	the	DT
cord-104279-choywmwd	265	22	activity	activity	NN
cord-104279-choywmwd	265	23	was	be	VBD
cord-104279-choywmwd	265	24	intracellular	intracellular	JJ
cord-104279-choywmwd	265	25	.	.	.
cord-104279-choywmwd	266	1	A	a	DT
cord-104279-choywmwd	266	2	-	-	HYPH
cord-104279-choywmwd	266	3	BB	bb	NN
cord-104279-choywmwd	266	4	fractionated	fractionate	VBN
cord-104279-choywmwd	266	5	with	with	IN
cord-104279-choywmwd	266	6	membranes	membrane	NNS
cord-104279-choywmwd	266	7	in	in	IN
cord-104279-choywmwd	266	8	the	the	DT
cord-104279-choywmwd	266	9	presence	presence	NN
cord-104279-choywmwd	266	10	of	of	IN
cord-104279-choywmwd	266	11	high	high	JJ
cord-104279-choywmwd	266	12	pH	pH	NNP
cord-104279-choywmwd	266	13	sodium	sodium	NN
cord-104279-choywmwd	266	14	carbonate	carbonate	NN
cord-104279-choywmwd	266	15	,	,	,
cord-104279-choywmwd	266	16	and	and	CC
cord-104279-choywmwd	266	17	thus	thus	RB
cord-104279-choywmwd	266	18	behaves	behave	VBZ
cord-104279-choywmwd	266	19	biochemically	biochemically	RB
cord-104279-choywmwd	266	20	as	as	IN
cord-104279-choywmwd	266	21	an	an	DT
cord-104279-choywmwd	266	22	integral	integral	JJ
cord-104279-choywmwd	266	23	membrane	membrane	NN
cord-104279-choywmwd	266	24	protein	protein	NN
cord-104279-choywmwd	266	25	(	(	-LRB-
cord-104279-choywmwd	266	26	data	datum	NNS
cord-104279-choywmwd	266	27	not	not	RB
cord-104279-choywmwd	266	28	shown	show	VBN
cord-104279-choywmwd	266	29	)	)	-RRB-
cord-104279-choywmwd	266	30	.	.	.
cord-104279-choywmwd	267	1	Indirect	indirect	JJ
cord-104279-choywmwd	267	2	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	267	3	microscopy	microscopy	NNS
cord-104279-choywmwd	267	4	using	use	VBG
cord-104279-choywmwd	267	5	anti	anti	JJ
cord-104279-choywmwd	267	6	-	-	JJ
cord-104279-choywmwd	267	7	DPAP	dpap	JJ
cord-104279-choywmwd	267	8	B	B	NNP
cord-104279-choywmwd	267	9	lumenal	lumenal	JJ
cord-104279-choywmwd	267	10	domain	domain	NN
cord-104279-choywmwd	267	11	antibody	antibody	NN
cord-104279-choywmwd	267	12	showed	show	VBD
cord-104279-choywmwd	267	13	that	that	IN
cord-104279-choywmwd	267	14	AA	AA	NNP
cord-104279-choywmwd	267	15	-	-	HYPH
cord-104279-choywmwd	267	16	B	B	NNP
cord-104279-choywmwd	267	17	and	and	CC
cord-104279-choywmwd	267	18	A	A	NNP
cord-104279-choywmwd	267	19	-	-	HYPH
cord-104279-choywmwd	267	20	BB	bb	NN
cord-104279-choywmwd	267	21	exhibited	exhibit	VBD
cord-104279-choywmwd	267	22	non	non	JJ
cord-104279-choywmwd	267	23	-	-	JJ
cord-104279-choywmwd	267	24	vacuolar	vacuolar	JJ
cord-104279-choywmwd	267	25	punctate	punctate	NN
cord-104279-choywmwd	267	26	staining	staining	NN
cord-104279-choywmwd	267	27	patterns	pattern	NNS
cord-104279-choywmwd	267	28	indistinguishable	indistinguishable	JJ
cord-104279-choywmwd	267	29	from	from	IN
cord-104279-choywmwd	267	30	wild	wild	JJ
cord-104279-choywmwd	267	31	type	type	NN
cord-104279-choywmwd	267	32	DPAP	DPAP	NNP
cord-104279-choywmwd	267	33	A	A	NNP
cord-104279-choywmwd	267	34	(	(	-LRB-
cord-104279-choywmwd	267	35	Fig	Fig	NNP
cord-104279-choywmwd	267	36	.	.	.
cord-104279-choywmwd	267	37	8	8	CD
cord-104279-choywmwd	267	38	,	,	,
cord-104279-choywmwd	267	39	A	A	NNP
cord-104279-choywmwd	267	40	-	-	HYPH
cord-104279-choywmwd	267	41	F	F	NNP
cord-104279-choywmwd	267	42	)	)	-RRB-
cord-104279-choywmwd	267	43	.	.	.
cord-104279-choywmwd	268	1	Again	again	RB
cord-104279-choywmwd	268	2	,	,	,
cord-104279-choywmwd	268	3	no	no	DT
cord-104279-choywmwd	268	4	plasma	plasma	NN
cord-104279-choywmwd	268	5	membrane	membrane	NN
cord-104279-choywmwd	268	6	staining	staining	NN
cord-104279-choywmwd	268	7	was	be	VBD
cord-104279-choywmwd	268	8	detected	detect	VBN
cord-104279-choywmwd	268	9	.	.	.
cord-104279-choywmwd	269	1	As	as	IN
cord-104279-choywmwd	269	2	with	with	IN
cord-104279-choywmwd	269	3	DPAP	DPAP	NNP
cord-104279-choywmwd	269	4	A	A	NNP
cord-104279-choywmwd	269	5	,	,	,
cord-104279-choywmwd	269	6	all	all	DT
cord-104279-choywmwd	269	7	cells	cell	NNS
cord-104279-choywmwd	269	8	that	that	WDT
cord-104279-choywmwd	269	9	showed	show	VBD
cord-104279-choywmwd	269	10	a	a	DT
cord-104279-choywmwd	269	11	signal	signal	NN
cord-104279-choywmwd	269	12	displayed	display	VBD
cord-104279-choywmwd	269	13	a	a	DT
cord-104279-choywmwd	269	14	punctate	punctate	NN
cord-104279-choywmwd	269	15	staining	staining	NN
cord-104279-choywmwd	269	16	pattern	pattern	NN
cord-104279-choywmwd	269	17	,	,	,
cord-104279-choywmwd	269	18	and	and	CC
cord-104279-choywmwd	269	19	a	a	DT
cord-104279-choywmwd	269	20	subset	subset	NN
cord-104279-choywmwd	269	21	of	of	IN
cord-104279-choywmwd	269	22	those	those	DT
cord-104279-choywmwd	269	23	cells	cell	NNS
cord-104279-choywmwd	269	24	that	that	WDT
cord-104279-choywmwd	269	25	showed	show	VBD
cord-104279-choywmwd	269	26	a	a	DT
cord-104279-choywmwd	269	27	strong	strong	JJ
cord-104279-choywmwd	269	28	signal	signal	NN
cord-104279-choywmwd	269	29	also	also	RB
cord-104279-choywmwd	269	30	exhibited	exhibit	VBD
cord-104279-choywmwd	269	31	vacuolar	vacuolar	NNP
cord-104279-choywmwd	269	32	localization	localization	NN
cord-104279-choywmwd	269	33	(	(	-LRB-
cord-104279-choywmwd	269	34	Table	table	NN
cord-104279-choywmwd	269	35	I	i	CD
cord-104279-choywmwd	269	36	)	)	-RRB-
cord-104279-choywmwd	269	37	.	.	.
cord-104279-choywmwd	270	1	An	an	DT
cord-104279-choywmwd	270	2	example	example	NN
cord-104279-choywmwd	270	3	is	be	VBZ
cord-104279-choywmwd	270	4	shown	show	VBN
cord-104279-choywmwd	270	5	in	in	IN
cord-104279-choywmwd	270	6	Fig	Fig	NNP
cord-104279-choywmwd	270	7	.	.	.
cord-104279-choywmwd	271	1	8	8	CD
cord-104279-choywmwd	272	1	(	(	-LRB-
cord-104279-choywmwd	272	2	A	A	NNP
cord-104279-choywmwd	272	3	-	-	HYPH
cord-104279-choywmwd	272	4	C	C	NNP
cord-104279-choywmwd	272	5	)	)	-RRB-
cord-104279-choywmwd	272	6	for	for	IN
cord-104279-choywmwd	272	7	cells	cell	NNS
cord-104279-choywmwd	272	8	expressing	express	VBG
cord-104279-choywmwd	272	9	AA	AA	NNP
cord-104279-choywmwd	272	10	-	-	HYPH
cord-104279-choywmwd	272	11	B.	B.	NNP
cord-104279-choywmwd	273	1	The	the	DT
cord-104279-choywmwd	273	2	cell	cell	NN
cord-104279-choywmwd	273	3	in	in	IN
cord-104279-choywmwd	273	4	the	the	DT
cord-104279-choywmwd	273	5	bottom	bottom	JJ
cord-104279-choywmwd	273	6	right	right	JJ
cord-104279-choywmwd	273	7	corner	corner	NN
cord-104279-choywmwd	273	8	shows	show	VBZ
cord-104279-choywmwd	273	9	a	a	DT
cord-104279-choywmwd	273	10	very	very	RB
cord-104279-choywmwd	273	11	intense	intense	JJ
cord-104279-choywmwd	273	12	fluorescein	fluorescein	NN
cord-104279-choywmwd	273	13	signal	signal	NN
cord-104279-choywmwd	273	14	relative	relative	JJ
cord-104279-choywmwd	273	15	to	to	IN
cord-104279-choywmwd	273	16	the	the	DT
cord-104279-choywmwd	273	17	other	other	JJ
cord-104279-choywmwd	273	18	cells	cell	NNS
cord-104279-choywmwd	273	19	in	in	IN
cord-104279-choywmwd	273	20	the	the	DT
cord-104279-choywmwd	273	21	panel	panel	NN
cord-104279-choywmwd	273	22	,	,	,
cord-104279-choywmwd	273	23	and	and	CC
cord-104279-choywmwd	273	24	clearly	clearly	RB
cord-104279-choywmwd	273	25	exhibits	exhibit	VBZ
cord-104279-choywmwd	273	26	co	co	NN
cord-104279-choywmwd	273	27	-	-	NN
cord-104279-choywmwd	273	28	localization	localization	NN
cord-104279-choywmwd	273	29	with	with	IN
cord-104279-choywmwd	273	30	Vat2p	Vat2p	NNP
cord-104279-choywmwd	273	31	.	.	.
cord-104279-choywmwd	274	1	Thus	thus	RB
cord-104279-choywmwd	274	2	,	,	,
cord-104279-choywmwd	274	3	as	as	IN
cord-104279-choywmwd	274	4	was	be	VBD
cord-104279-choywmwd	274	5	seen	see	VBN
cord-104279-choywmwd	274	6	for	for	IN
cord-104279-choywmwd	274	7	wild	wild	JJ
cord-104279-choywmwd	274	8	type	type	NN
cord-104279-choywmwd	274	9	DPAP	DPAP	NNP
cord-104279-choywmwd	274	10	A	A	NNP
cord-104279-choywmwd	274	11	,	,	,
cord-104279-choywmwd	274	12	overproduction	overproduction	NN
cord-104279-choywmwd	274	13	of	of	IN
cord-104279-choywmwd	274	14	these	these	DT
cord-104279-choywmwd	274	15	proteins	protein	NNS
cord-104279-choywmwd	274	16	resulted	result	VBD
cord-104279-choywmwd	274	17	in	in	IN
cord-104279-choywmwd	274	18	some	some	DT
cord-104279-choywmwd	274	19	mislocalization	mislocalization	NN
cord-104279-choywmwd	274	20	to	to	IN
cord-104279-choywmwd	274	21	the	the	DT
cord-104279-choywmwd	274	22	vacuole	vacuole	NN
cord-104279-choywmwd	274	23	.	.	.
cord-104279-choywmwd	275	1	These	these	DT
cord-104279-choywmwd	275	2	experiments	experiment	NNS
cord-104279-choywmwd	275	3	demonstrate	demonstrate	VBP
cord-104279-choywmwd	275	4	that	that	IN
cord-104279-choywmwd	275	5	the	the	DT
cord-104279-choywmwd	275	6	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	275	7	domain	domain	NN
cord-104279-choywmwd	275	8	of	of	IN
cord-104279-choywmwd	275	9	DPAP	DPAP	NNP
cord-104279-choywmwd	275	10	A	A	NNP
cord-104279-choywmwd	275	11	is	be	VBZ
cord-104279-choywmwd	275	12	sufficient	sufficient	JJ
cord-104279-choywmwd	275	13	for	for	IN
cord-104279-choywmwd	275	14	the	the	DT
cord-104279-choywmwd	275	15	retention	retention	NN
cord-104279-choywmwd	275	16	of	of	IN
cord-104279-choywmwd	275	17	an	an	DT
cord-104279-choywmwd	275	18	otherwise	otherwise	RB
cord-104279-choywmwd	275	19	vacuolar	vacuolar	JJ
cord-104279-choywmwd	275	20	membrane	membrane	NN
cord-104279-choywmwd	275	21	protein	protein	NN
cord-104279-choywmwd	275	22	in	in	IN
cord-104279-choywmwd	275	23	punctate	punctate	NN
cord-104279-choywmwd	275	24	structures	structure	NNS
cord-104279-choywmwd	275	25	typical	typical	JJ
cord-104279-choywmwd	275	26	of	of	IN
cord-104279-choywmwd	275	27	the	the	DT
cord-104279-choywmwd	275	28	Golgi	Golgi	NNP
cord-104279-choywmwd	275	29	apparatus	apparatus	NN
cord-104279-choywmwd	275	30	in	in	IN
cord-104279-choywmwd	275	31	S.	S.	NNP
cord-104279-choywmwd	275	32	cerevisiae	cerevisiae	NNS
cord-104279-choywmwd	275	33	.	.	.
cord-104279-choywmwd	276	1	The	the	DT
cord-104279-choywmwd	276	2	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	276	3	domain	domain	NN
cord-104279-choywmwd	276	4	of	of	IN
cord-104279-choywmwd	276	5	DPAP	DPAP	NNP
cord-104279-choywmwd	276	6	A	A	NNP
cord-104279-choywmwd	276	7	also	also	RB
cord-104279-choywmwd	276	8	has	have	VBZ
cord-104279-choywmwd	276	9	been	be	VBN
cord-104279-choywmwd	276	10	shown	show	VBN
cord-104279-choywmwd	276	11	to	to	TO
cord-104279-choywmwd	276	12	be	be	VB
cord-104279-choywmwd	276	13	sufficient	sufficient	JJ
cord-104279-choywmwd	276	14	for	for	IN
cord-104279-choywmwd	276	15	Golgi	Golgi	NNP
cord-104279-choywmwd	276	16	retention	retention	NN
cord-104279-choywmwd	276	17	when	when	WRB
cord-104279-choywmwd	276	18	fused	fuse	VBN
cord-104279-choywmwd	276	19	to	to	IN
cord-104279-choywmwd	276	20	the	the	DT
cord-104279-choywmwd	276	21	transmembrane	transmembrane	NN
cord-104279-choywmwd	276	22	and	and	CC
cord-104279-choywmwd	276	23	lumenal	lumenal	JJ
cord-104279-choywmwd	276	24	domains	domain	NNS
cord-104279-choywmwd	276	25	of	of	IN
cord-104279-choywmwd	276	26	the	the	DT
cord-104279-choywmwd	276	27	vacuolar	vacuolar	NNP
cord-104279-choywmwd	276	28	membrane	membrane	NN
cord-104279-choywmwd	276	29	protein	protein	NN
cord-104279-choywmwd	276	30	ALP	ALP	NNP
cord-104279-choywmwd	276	31	(	(	-LRB-
cord-104279-choywmwd	276	32	S.	S.	NNP
cord-104279-choywmwd	276	33	Nothwehr	Nothwehr	NNP
cord-104279-choywmwd	276	34	and	and	CC
cord-104279-choywmwd	276	35	1	1	CD
cord-104279-choywmwd	276	36	"	"	''
cord-104279-choywmwd	276	37	.	.	.
cord-104279-choywmwd	277	1	Stevens	Stevens	NNP
cord-104279-choywmwd	277	2	,	,	,
cord-104279-choywmwd	277	3	unpublished	unpublished	JJ
cord-104279-choywmwd	277	4	data	datum	NNS
cord-104279-choywmwd	277	5	)	)	-RRB-
cord-104279-choywmwd	277	6	.	.	.
cord-104279-choywmwd	278	1	To	to	TO
cord-104279-choywmwd	278	2	determine	determine	VB
cord-104279-choywmwd	278	3	if	if	IN
cord-104279-choywmwd	278	4	the	the	DT
cord-104279-choywmwd	278	5	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	278	6	domain	domain	NN
cord-104279-choywmwd	278	7	of	of	IN
cord-104279-choywmwd	278	8	DPAP	DPAP	NNP
cord-104279-choywmwd	278	9	A	A	NNP
cord-104279-choywmwd	278	10	was	be	VBD
cord-104279-choywmwd	278	11	also	also	RB
cord-104279-choywmwd	278	12	necessary	necessary	JJ
cord-104279-choywmwd	278	13	for	for	IN
cord-104279-choywmwd	278	14	the	the	DT
cord-104279-choywmwd	278	15	retention	retention	NN
cord-104279-choywmwd	278	16	of	of	IN
cord-104279-choywmwd	278	17	DPAP	DPAP	NNP
cord-104279-choywmwd	278	18	A	A	NNP
cord-104279-choywmwd	278	19	in	in	IN
cord-104279-choywmwd	278	20	the	the	DT
cord-104279-choywmwd	278	21	Golgi	Golgi	NNP
cord-104279-choywmwd	278	22	,	,	,
cord-104279-choywmwd	278	23	a	a	DT
cord-104279-choywmwd	278	24	series	series	NN
cord-104279-choywmwd	278	25	of	of	IN
cord-104279-choywmwd	278	26	in	in	IN
cord-104279-choywmwd	278	27	-	-	HYPH
cord-104279-choywmwd	278	28	frame	frame	NN
cord-104279-choywmwd	278	29	deletion	deletion	NN
cord-104279-choywmwd	278	30	mutations	mutation	NNS
cord-104279-choywmwd	278	31	in	in	IN
cord-104279-choywmwd	278	32	the	the	DT
cord-104279-choywmwd	278	33	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	278	34	domain	domain	NN
cord-104279-choywmwd	278	35	were	be	VBD
cord-104279-choywmwd	278	36	generated	generate	VBN
cord-104279-choywmwd	278	37	using	use	VBG
cord-104279-choywmwd	278	38	oligonucleotide	oligonucleotide	RB
cord-104279-choywmwd	278	39	-	-	HYPH
cord-104279-choywmwd	278	40	directed	direct	VBN
cord-104279-choywmwd	278	41	mutagenesis	mutagenesis	NN
cord-104279-choywmwd	278	42	(	(	-LRB-
cord-104279-choywmwd	278	43	see	see	VB
cord-104279-choywmwd	278	44	Materials	material	NNS
cord-104279-choywmwd	278	45	and	and	CC
cord-104279-choywmwd	278	46	Methods	Methods	NNPS
cord-104279-choywmwd	278	47	)	)	-RRB-
cord-104279-choywmwd	278	48	.	.	.
cord-104279-choywmwd	279	1	Deletion	deletion	NN
cord-104279-choywmwd	279	2	variants	variant	NNS
cord-104279-choywmwd	279	3	that	that	WDT
cord-104279-choywmwd	279	4	lacked	lack	VBD
cord-104279-choywmwd	279	5	(	(	-LRB-
cord-104279-choywmwd	279	6	Fig	Fig	NNP
cord-104279-choywmwd	279	7	.	.	.
cord-104279-choywmwd	279	8	9	9	CD
cord-104279-choywmwd	279	9	)	)	-RRB-
cord-104279-choywmwd	279	10	,	,	,
cord-104279-choywmwd	279	11	although	although	IN
cord-104279-choywmwd	279	12	both	both	DT
cord-104279-choywmwd	279	13	proteins	protein	NNS
cord-104279-choywmwd	279	14	also	also	RB
cord-104279-choywmwd	279	15	showed	show	VBD
cord-104279-choywmwd	279	16	a	a	DT
cord-104279-choywmwd	279	17	small	small	JJ
cord-104279-choywmwd	279	18	amount	amount	NN
cord-104279-choywmwd	279	19	of	of	IN
cord-104279-choywmwd	279	20	ER	er	NN
cord-104279-choywmwd	279	21	staining	staining	NN
cord-104279-choywmwd	279	22	(	(	-LRB-
cord-104279-choywmwd	279	23	Table	table	NN
cord-104279-choywmwd	279	24	I	i	CD
cord-104279-choywmwd	279	25	)	)	-RRB-
cord-104279-choywmwd	279	26	.	.	.
cord-104279-choywmwd	280	1	Immunoblotting	Immunoblotting	NNP
cord-104279-choywmwd	280	2	analysis	analysis	NN
cord-104279-choywmwd	280	3	of	of	IN
cord-104279-choywmwd	280	4	crude	crude	JJ
cord-104279-choywmwd	280	5	extracts	extract	NNS
cord-104279-choywmwd	280	6	prepared	prepare	VBN
cord-104279-choywmwd	280	7	from	from	IN
cord-104279-choywmwd	280	8	cells	cell	NNS
cord-104279-choywmwd	280	9	expressing	express	VBG
cord-104279-choywmwd	280	10	A22-AA	A22-AA	NNP
cord-104279-choywmwd	280	11	-	-	HYPH
cord-104279-choywmwd	280	12	B	B	NNP
cord-104279-choywmwd	280	13	showed	show	VBD
cord-104279-choywmwd	280	14	a	a	DT
cord-104279-choywmwd	280	15	broadly	broadly	RB
cord-104279-choywmwd	280	16	migrating	migrate	VBG
cord-104279-choywmwd	280	17	species	specie	NNS
cord-104279-choywmwd	280	18	on	on	IN
cord-104279-choywmwd	280	19	SDS	SDS	NNP
cord-104279-choywmwd	280	20	-	-	HYPH
cord-104279-choywmwd	280	21	PAGE	PAGE	NNP
cord-104279-choywmwd	280	22	similar	similar	JJ
cord-104279-choywmwd	280	23	to	to	IN
cord-104279-choywmwd	280	24	AA	AA	NNP
cord-104279-choywmwd	280	25	-	-	HYPH
cord-104279-choywmwd	280	26	B	B	NNP
cord-104279-choywmwd	280	27	and	and	CC
cord-104279-choywmwd	280	28	A	A	NNP
cord-104279-choywmwd	280	29	-	-	HYPH
cord-104279-choywmwd	280	30	BB	bb	NN
cord-104279-choywmwd	281	1	(	(	-LRB-
cord-104279-choywmwd	281	2	data	datum	NNS
cord-104279-choywmwd	281	3	not	not	RB
cord-104279-choywmwd	281	4	shown	show	VBN
cord-104279-choywmwd	281	5	;	;	:
cord-104279-choywmwd	281	6	Fig	Fig	NNP
cord-104279-choywmwd	281	7	.	.	.
cord-104279-choywmwd	281	8	6	6	CD
cord-104279-choywmwd	281	9	B	b	NN
cord-104279-choywmwd	281	10	)	)	-RRB-
cord-104279-choywmwd	281	11	,	,	,
cord-104279-choywmwd	281	12	suggesting	suggest	VBG
cord-104279-choywmwd	281	13	that	that	IN
cord-104279-choywmwd	281	14	the	the	DT
cord-104279-choywmwd	281	15	A22-AA	A22-AA	NNP
cord-104279-choywmwd	281	16	-	-	HYPH
cord-104279-choywmwd	281	17	B	b	NN
cord-104279-choywmwd	281	18	protein	protein	NN
cord-104279-choywmwd	281	19	transits	transit	VBZ
cord-104279-choywmwd	281	20	the	the	DT
cord-104279-choywmwd	281	21	Golgi	Golgi	NNP
cord-104279-choywmwd	281	22	apparatus	apparatus	NN
cord-104279-choywmwd	281	23	before	before	IN
cord-104279-choywmwd	281	24	its	-PRON-	PRP$
cord-104279-choywmwd	281	25	transport	transport	NN
cord-104279-choywmwd	281	26	to	to	IN
cord-104279-choywmwd	281	27	the	the	DT
cord-104279-choywmwd	281	28	vacuole	vacuole	NN
cord-104279-choywmwd	281	29	.	.	.
cord-104279-choywmwd	282	1	Similar	similar	JJ
cord-104279-choywmwd	282	2	to	to	IN
cord-104279-choywmwd	282	3	the	the	DT
cord-104279-choywmwd	282	4	deletions	deletion	NNS
cord-104279-choywmwd	282	5	in	in	IN
cord-104279-choywmwd	282	6	the	the	DT
cord-104279-choywmwd	282	7	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	282	8	domain	domain	NN
cord-104279-choywmwd	282	9	of	of	IN
cord-104279-choywmwd	282	10	Figure	figure	NN
cord-104279-choywmwd	282	11	8	8	CD
cord-104279-choywmwd	282	12	.	.	.
cord-104279-choywmwd	283	1	Immunolocalization	Immunolocalization	NNP
cord-104279-choywmwd	283	2	of	of	IN
cord-104279-choywmwd	283	3	AA	AA	NNP
cord-104279-choywmwd	283	4	-	-	HYPH
cord-104279-choywmwd	283	5	B	B	NNP
cord-104279-choywmwd	283	6	and	and	CC
cord-104279-choywmwd	283	7	A	A	NNP
cord-104279-choywmwd	283	8	-	-	HYPH
cord-104279-choywmwd	283	9	BB	bb	NN
cord-104279-choywmwd	283	10	.	.	.
cord-104279-choywmwd	284	1	JHRY'20	JHRY'20	NNP
cord-104279-choywmwd	284	2	-	-	HYPH
cord-104279-choywmwd	284	3	1A	1A	NNP
cord-104279-choywmwd	284	4	(	(	-LRB-
cord-104279-choywmwd	284	5	ste13A	ste13A	NNP
cord-104279-choywmwd	284	6	dap2A	dap2A	NNP
cord-104279-choywmwd	284	7	)	)	-RRB-
cord-104279-choywmwd	284	8	cells	cell	NNS
cord-104279-choywmwd	284	9	containing	contain	VBG
cord-104279-choywmwd	284	10	plasmids	plasmid	NNS
cord-104279-choywmwd	284	11	expressing	express	VBG
cord-104279-choywmwd	284	12	either	either	CC
cord-104279-choywmwd	284	13	AA	AA	NNP
cord-104279-choywmwd	284	14	-	-	HYPH
cord-104279-choywmwd	284	15	B	B	NNP
cord-104279-choywmwd	284	16	(	(	-LRB-
cord-104279-choywmwd	284	17	pCJR64	pCJR64	NNP
cord-104279-choywmwd	284	18	,	,	,
cord-104279-choywmwd	284	19	A	A	NNP
cord-104279-choywmwd	284	20	-	-	HYPH
cord-104279-choywmwd	284	21	C	C	NNP
cord-104279-choywmwd	284	22	)	)	-RRB-
cord-104279-choywmwd	284	23	or	or	CC
cord-104279-choywmwd	284	24	A	a	DT
cord-104279-choywmwd	284	25	-	-	HYPH
cord-104279-choywmwd	284	26	BB	bb	NN
cord-104279-choywmwd	284	27	(	(	-LRB-
cord-104279-choywmwd	284	28	pCJR67	pCJR67	NNP
cord-104279-choywmwd	284	29	,	,	,
cord-104279-choywmwd	284	30	D	D	NNP
cord-104279-choywmwd	284	31	-	-	HYPH
cord-104279-choywmwd	284	32	F	F	NNP
cord-104279-choywmwd	284	33	)	)	-RRB-
cord-104279-choywmwd	284	34	were	be	VBD
cord-104279-choywmwd	284	35	fixed	fix	VBN
cord-104279-choywmwd	284	36	,	,	,
cord-104279-choywmwd	284	37	spheroplasted	spheroplasted	JJ
cord-104279-choywmwd	284	38	,	,	,
cord-104279-choywmwd	284	39	and	and	CC
cord-104279-choywmwd	284	40	stained	stain	VBN
cord-104279-choywmwd	284	41	with	with	IN
cord-104279-choywmwd	284	42	DPAP	DPAP	NNP
cord-104279-choywmwd	284	43	A	a	DT
cord-104279-choywmwd	284	44	antibody	antibody	NN
cord-104279-choywmwd	284	45	and	and	CC
cord-104279-choywmwd	284	46	Vat2p	vat2p	RB
cord-104279-choywmwd	284	47	mAb	mab	NN
cord-104279-choywmwd	284	48	.	.	.
cord-104279-choywmwd	285	1	The	the	DT
cord-104279-choywmwd	285	2	cells	cell	NNS
cord-104279-choywmwd	285	3	were	be	VBD
cord-104279-choywmwd	285	4	viewed	view	VBN
cord-104279-choywmwd	285	5	by	by	IN
cord-104279-choywmwd	285	6	Nomarski	Nomarski	NNP
cord-104279-choywmwd	285	7	optics	optic	NNS
cord-104279-choywmwd	285	8	(	(	-LRB-
cord-104279-choywmwd	285	9	A	A	NNP
cord-104279-choywmwd	285	10	and	and	CC
cord-104279-choywmwd	285	11	C	C	NNP
cord-104279-choywmwd	285	12	)	)	-RRB-
cord-104279-choywmwd	285	13	and	and	CC
cord-104279-choywmwd	285	14	by	by	IN
cord-104279-choywmwd	285	15	epifluorescence	epifluorescence	NN
cord-104279-choywmwd	285	16	using	use	VBG
cord-104279-choywmwd	285	17	filter	filter	NN
cord-104279-choywmwd	285	18	sets	set	NNS
cord-104279-choywmwd	285	19	specific	specific	JJ
cord-104279-choywmwd	285	20	for	for	IN
cord-104279-choywmwd	285	21	fluorescein	fluorescein	NN
cord-104279-choywmwd	285	22	(	(	-LRB-
cord-104279-choywmwd	285	23	B	b	NN
cord-104279-choywmwd	285	24	and	and	CC
cord-104279-choywmwd	285	25	E	e	NN
cord-104279-choywmwd	285	26	)	)	-RRB-
cord-104279-choywmwd	285	27	and	and	CC
cord-104279-choywmwd	285	28	rhodamine	rhodamine	JJ
cord-104279-choywmwd	285	29	(	(	-LRB-
cord-104279-choywmwd	285	30	C	C	NNP
cord-104279-choywmwd	285	31	and	and	CC
cord-104279-choywmwd	285	32	F	F	NNP
cord-104279-choywmwd	285	33	)	)	-RRB-
cord-104279-choywmwd	285	34	fluorescence	fluorescence	NN
cord-104279-choywmwd	285	35	.	.	.
cord-104279-choywmwd	286	1	DPAP	DPAP	NNP
cord-104279-choywmwd	286	2	B	B	NNP
cord-104279-choywmwd	286	3	,	,	,
cord-104279-choywmwd	286	4	this	this	DT
cord-104279-choywmwd	286	5	deletion	deletion	NN
cord-104279-choywmwd	286	6	delays	delay	VBZ
cord-104279-choywmwd	286	7	exit	exit	NN
cord-104279-choywmwd	286	8	of	of	IN
cord-104279-choywmwd	286	9	the	the	DT
cord-104279-choywmwd	286	10	fusion	fusion	NN
cord-104279-choywmwd	286	11	proteins	protein	NNS
cord-104279-choywmwd	286	12	from	from	IN
cord-104279-choywmwd	286	13	the	the	DT
cord-104279-choywmwd	286	14	ER	er	NN
cord-104279-choywmwd	286	15	;	;	:
cord-104279-choywmwd	286	16	however	however	RB
cord-104279-choywmwd	286	17	,	,	,
cord-104279-choywmwd	286	18	upon	upon	IN
cord-104279-choywmwd	286	19	exit	exit	NN
cord-104279-choywmwd	286	20	from	from	IN
cord-104279-choywmwd	286	21	the	the	DT
cord-104279-choywmwd	286	22	ER	ER	NNP
cord-104279-choywmwd	286	23	,	,	,
cord-104279-choywmwd	286	24	the	the	DT
cord-104279-choywmwd	286	25	enzymatically	enzymatically	RB
cord-104279-choywmwd	286	26	active	active	JJ
cord-104279-choywmwd	286	27	A22-AAA	A22-AAA	,
cord-104279-choywmwd	286	28	and	and	CC
cord-104279-choywmwd	286	29	A22-AA	a22-aa	NN
cord-104279-choywmwd	286	30	-	-	HYPH
cord-104279-choywmwd	286	31	B	b	NN
cord-104279-choywmwd	286	32	fusion	fusion	NN
cord-104279-choywmwd	286	33	proteins	protein	NNS
cord-104279-choywmwd	286	34	were	be	VBD
cord-104279-choywmwd	286	35	transported	transport	VBN
cord-104279-choywmwd	286	36	to	to	IN
cord-104279-choywmwd	286	37	the	the	DT
cord-104279-choywmwd	286	38	vacuole	vacuole	NN
cord-104279-choywmwd	286	39	.	.	.
cord-104279-choywmwd	287	1	Thus	thus	RB
cord-104279-choywmwd	287	2	,	,	,
cord-104279-choywmwd	287	3	the	the	DT
cord-104279-choywmwd	287	4	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	287	5	domain	domain	NN
cord-104279-choywmwd	287	6	is	be	VBZ
cord-104279-choywmwd	287	7	both	both	DT
cord-104279-choywmwd	287	8	necessary	necessary	JJ
cord-104279-choywmwd	287	9	and	and	CC
cord-104279-choywmwd	287	10	sufficient	sufficient	JJ
cord-104279-choywmwd	287	11	for	for	IN
cord-104279-choywmwd	287	12	the	the	DT
cord-104279-choywmwd	287	13	retention	retention	NN
cord-104279-choywmwd	287	14	of	of	IN
cord-104279-choywmwd	287	15	DPAP	DPAP	NNP
cord-104279-choywmwd	287	16	A	A	NNP
cord-104279-choywmwd	287	17	in	in	IN
cord-104279-choywmwd	287	18	the	the	DT
cord-104279-choywmwd	287	19	Golgi	Golgi	NNP
cord-104279-choywmwd	287	20	apparatus	apparatus	NN
cord-104279-choywmwd	287	21	,	,	,
cord-104279-choywmwd	287	22	and	and	CC
cord-104279-choywmwd	287	23	the	the	DT
cord-104279-choywmwd	287	24	signal	signal	NN
cord-104279-choywmwd	287	25	for	for	IN
cord-104279-choywmwd	287	26	the	the	DT
cord-104279-choywmwd	287	27	retention	retention	NN
cord-104279-choywmwd	287	28	of	of	IN
cord-104279-choywmwd	287	29	DPAP	DPAP	NNP
cord-104279-choywmwd	287	30	A	A	NNP
cord-104279-choywmwd	287	31	maps	map	NNS
cord-104279-choywmwd	287	32	to	to	IN
cord-104279-choywmwd	287	33	a	a	DT
cord-104279-choywmwd	287	34	22-amino	22-amino	CD
cord-104279-choywmwd	287	35	acid	acid	NN
cord-104279-choywmwd	287	36	segment	segment	NN
cord-104279-choywmwd	287	37	within	within	IN
cord-104279-choywmwd	287	38	the	the	DT
cord-104279-choywmwd	287	39	ll8-residue	ll8-residue	JJ
cord-104279-choywmwd	287	40	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	287	41	domain	domain	NN
cord-104279-choywmwd	287	42	.	.	.
cord-104279-choywmwd	288	1	We	-PRON-	PRP
cord-104279-choywmwd	288	2	have	have	VBP
cord-104279-choywmwd	288	3	previously	previously	RB
cord-104279-choywmwd	288	4	shown	show	VBN
cord-104279-choywmwd	288	5	that	that	IN
cord-104279-choywmwd	288	6	the	the	DT
cord-104279-choywmwd	288	7	transport	transport	NN
cord-104279-choywmwd	288	8	of	of	IN
cord-104279-choywmwd	288	9	DPAP	DPAP	NNP
cord-104279-choywmwd	288	10	B	B	NNP
cord-104279-choywmwd	288	11	to	to	IN
cord-104279-choywmwd	288	12	the	the	DT
cord-104279-choywmwd	288	13	vacuole	vacuole	NN
cord-104279-choywmwd	288	14	does	do	VBZ
cord-104279-choywmwd	288	15	not	not	RB
cord-104279-choywmwd	288	16	involve	involve	VB
cord-104279-choywmwd	288	17	delivery	delivery	NN
cord-104279-choywmwd	288	18	to	to	IN
cord-104279-choywmwd	288	19	the	the	DT
cord-104279-choywmwd	288	20	plasma	plasma	NN
cord-104279-choywmwd	288	21	membrane	membrane	NN
cord-104279-choywmwd	288	22	followed	follow	VBN
cord-104279-choywmwd	288	23	by	by	IN
cord-104279-choywmwd	288	24	endocytic	endocytic	JJ
cord-104279-choywmwd	288	25	targeting	targeting	NN
cord-104279-choywmwd	288	26	to	to	IN
cord-104279-choywmwd	288	27	the	the	DT
cord-104279-choywmwd	288	28	vacuole	vacuole	NN
cord-104279-choywmwd	288	29	(	(	-LRB-
cord-104279-choywmwd	288	30	Roberrs	Roberrs	NNP
cord-104279-choywmwd	288	31	et	et	NNP
cord-104279-choywmwd	288	32	al	al	NNP
cord-104279-choywmwd	288	33	.	.	NNP
cord-104279-choywmwd	288	34	,	,	,
cord-104279-choywmwd	288	35	1989	1989	CD
cord-104279-choywmwd	288	36	)	)	-RRB-
cord-104279-choywmwd	288	37	.	.	.
cord-104279-choywmwd	289	1	This	this	DT
cord-104279-choywmwd	289	2	was	be	VBD
cord-104279-choywmwd	289	3	demonstrated	demonstrate	VBN
cord-104279-choywmwd	289	4	by	by	IN
cord-104279-choywmwd	289	5	expressing	express	VBG
cord-104279-choywmwd	289	6	DPAP	DPAP	NNP
cord-104279-choywmwd	289	7	B	B	NNP
cord-104279-choywmwd	289	8	in	in	IN
cord-104279-choywmwd	289	9	a	a	DT
cord-104279-choywmwd	289	10	secl	secl	JJ
cord-104279-choywmwd	289	11	mutant	mutant	NN
cord-104279-choywmwd	289	12	,	,	,
cord-104279-choywmwd	289	13	which	which	WDT
cord-104279-choywmwd	289	14	at	at	IN
cord-104279-choywmwd	289	15	34~	34~	CD
cord-104279-choywmwd	289	16	is	be	VBZ
cord-104279-choywmwd	289	17	blocked	block	VBN
cord-104279-choywmwd	289	18	at	at	IN
cord-104279-choywmwd	289	19	a	a	DT
cord-104279-choywmwd	289	20	late	late	JJ
cord-104279-choywmwd	289	21	stage	stage	NN
cord-104279-choywmwd	289	22	of	of	IN
cord-104279-choywmwd	289	23	the	the	DT
cord-104279-choywmwd	289	24	secretory	secretory	JJ
cord-104279-choywmwd	289	25	pathway	pathway	NN
cord-104279-choywmwd	289	26	and	and	CC
cord-104279-choywmwd	289	27	accumulates	accumulate	VBZ
cord-104279-choywmwd	289	28	secretory	secretory	JJ
cord-104279-choywmwd	289	29	vesicles	vesicle	NNS
cord-104279-choywmwd	290	1	Salminen	Salminen	NNP
cord-104279-choywmwd	290	2	et	et	NNP
cord-104279-choywmwd	290	3	al	al	NNP
cord-104279-choywmwd	290	4	.	.	NNP
cord-104279-choywmwd	290	5	,	,	,
cord-104279-choywmwd	290	6	1987	1987	CD
cord-104279-choywmwd	290	7	)	)	-RRB-
cord-104279-choywmwd	290	8	.	.	.
cord-104279-choywmwd	291	1	To	to	IN
cord-104279-choywmwd	291	2	ad	ad	NN
cord-104279-choywmwd	291	3	-	-	HYPH
cord-104279-choywmwd	291	4	dress	dress	VB
cord-104279-choywmwd	291	5	the	the	DT
cord-104279-choywmwd	291	6	possibility	possibility	NN
cord-104279-choywmwd	291	7	that	that	IN
cord-104279-choywmwd	291	8	the	the	DT
cord-104279-choywmwd	291	9	mutant	mutant	JJ
cord-104279-choywmwd	291	10	constructs	construct	NNS
cord-104279-choywmwd	291	11	analyzed	analyze	VBN
cord-104279-choywmwd	291	12	in	in	IN
cord-104279-choywmwd	291	13	this	this	DT
cord-104279-choywmwd	291	14	study	study	NN
cord-104279-choywmwd	291	15	were	be	VBD
cord-104279-choywmwd	291	16	mislocalized	mislocalize	VBN
cord-104279-choywmwd	291	17	to	to	IN
cord-104279-choywmwd	291	18	the	the	DT
cord-104279-choywmwd	291	19	plasma	plasma	NN
cord-104279-choywmwd	291	20	membrane	membrane	NN
cord-104279-choywmwd	291	21	followed	follow	VBN
cord-104279-choywmwd	291	22	by	by	IN
cord-104279-choywmwd	291	23	rapid	rapid	JJ
cord-104279-choywmwd	291	24	endocytic	endocytic	JJ
cord-104279-choywmwd	291	25	uptake	uptake	NN
cord-104279-choywmwd	291	26	to	to	IN
cord-104279-choywmwd	291	27	the	the	DT
cord-104279-choywmwd	291	28	vacuole	vacuole	NN
cord-104279-choywmwd	291	29	,	,	,
cord-104279-choywmwd	291	30	indirect	indirect	JJ
cord-104279-choywmwd	291	31	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	291	32	experiments	experiment	NNS
cord-104279-choywmwd	291	33	were	be	VBD
cord-104279-choywmwd	291	34	performed	perform	VBN
cord-104279-choywmwd	291	35	on	on	IN
cord-104279-choywmwd	291	36	BB	BB	NNP
cord-104279-choywmwd	291	37	-	-	HYPH
cord-104279-choywmwd	291	38	Inv	Inv	NNP
cord-104279-choywmwd	291	39	,	,	,
cord-104279-choywmwd	291	40	B	b	NN
cord-104279-choywmwd	291	41	-	-	HYPH
cord-104279-choywmwd	291	42	A	A	NNP
cord-104279-choywmwd	291	43	-	-	HYPH
cord-104279-choywmwd	291	44	B	b	NN
cord-104279-choywmwd	291	45	,	,	,
cord-104279-choywmwd	291	46	A20-BB	A20-BB	NNP
cord-104279-choywmwd	291	47	,	,	,
cord-104279-choywmwd	291	48	and	and	CC
cord-104279-choywmwd	291	49	/	/	SYM
cord-104279-choywmwd	291	50	x22-AA	x22-AA	NNP
cord-104279-choywmwd	291	51	-	-	HYPH
cord-104279-choywmwd	291	52	B	B	NNP
cord-104279-choywmwd	291	53	expressed	express	VBD
cord-104279-choywmwd	291	54	in	in	IN
cord-104279-choywmwd	291	55	a	a	DT
cord-104279-choywmwd	291	56	secl	secl	JJ
cord-104279-choywmwd	291	57	strain	strain	NN
cord-104279-choywmwd	291	58	at	at	IN
cord-104279-choywmwd	291	59	34~	34~	CD
cord-104279-choywmwd	292	1	As	as	IN
cord-104279-choywmwd	292	2	a	a	DT
cord-104279-choywmwd	292	3	positive	positive	JJ
cord-104279-choywmwd	292	4	control	control	NN
cord-104279-choywmwd	292	5	for	for	IN
cord-104279-choywmwd	292	6	accumulation	accumulation	NN
cord-104279-choywmwd	292	7	in	in	IN
cord-104279-choywmwd	292	8	secretory	secretory	JJ
cord-104279-choywmwd	292	9	vesicles	vesicle	NNS
cord-104279-choywmwd	292	10	,	,	,
cord-104279-choywmwd	292	11	the	the	DT
cord-104279-choywmwd	292	12	localization	localization	NN
cord-104279-choywmwd	292	13	of	of	IN
cord-104279-choywmwd	292	14	the	the	DT
cord-104279-choywmwd	292	15	fusion	fusion	NN
cord-104279-choywmwd	292	16	protein	protein	NN
cord-104279-choywmwd	292	17	Fusl	Fusl	NNP
cord-104279-choywmwd	292	18	-	-	HYPH
cord-104279-choywmwd	292	19	LacZp	LacZp	NNP
cord-104279-choywmwd	292	20	was	be	VBD
cord-104279-choywmwd	292	21	analyzed	analyze	VBN
cord-104279-choywmwd	293	1	(	(	-LRB-
cord-104279-choywmwd	293	2	Trueheart	Trueheart	NNP
cord-104279-choywmwd	293	3	et	et	NNP
cord-104279-choywmwd	293	4	al	al	NNP
cord-104279-choywmwd	293	5	.	.	NNP
cord-104279-choywmwd	293	6	,	,	,
cord-104279-choywmwd	293	7	1987	1987	CD
cord-104279-choywmwd	293	8	)	)	-RRB-
cord-104279-choywmwd	293	9	.	.	.
cord-104279-choywmwd	294	1	The	the	DT
cord-104279-choywmwd	294	2	FUS1	FUS1	NNP
cord-104279-choywmwd	294	3	gene	gene	NN
cord-104279-choywmwd	294	4	product	product	NN
cord-104279-choywmwd	294	5	is	be	VBZ
cord-104279-choywmwd	294	6	required	require	VBN
cord-104279-choywmwd	294	7	for	for	IN
cord-104279-choywmwd	294	8	the	the	DT
cord-104279-choywmwd	294	9	breakdown	breakdown	NN
cord-104279-choywmwd	294	10	of	of	IN
cord-104279-choywmwd	294	11	the	the	DT
cord-104279-choywmwd	294	12	cell	cell	NN
cord-104279-choywmwd	294	13	walls	wall	NNS
cord-104279-choywmwd	294	14	after	after	IN
cord-104279-choywmwd	294	15	the	the	DT
cord-104279-choywmwd	294	16	fusion	fusion	NN
cord-104279-choywmwd	294	17	of	of	IN
cord-104279-choywmwd	294	18	a	a	DT
cord-104279-choywmwd	294	19	and	and	CC
cord-104279-choywmwd	294	20	ct	ct	NN
cord-104279-choywmwd	294	21	cells	cell	NNS
cord-104279-choywmwd	294	22	during	during	IN
cord-104279-choywmwd	294	23	conjugation	conjugation	NN
cord-104279-choywmwd	294	24	(	(	-LRB-
cord-104279-choywmwd	294	25	McCaffrey	McCaffrey	NNP
cord-104279-choywmwd	294	26	et	et	NNP
cord-104279-choywmwd	294	27	al	al	NNP
cord-104279-choywmwd	294	28	.	.	NNP
cord-104279-choywmwd	294	29	,	,	,
cord-104279-choywmwd	294	30	1987	1987	CD
cord-104279-choywmwd	294	31	;	;	:
cord-104279-choywmwd	294	32	Trueheart	Trueheart	NNP
cord-104279-choywmwd	294	33	et	et	NNP
cord-104279-choywmwd	294	34	al	al	NNP
cord-104279-choywmwd	294	35	.	.	NNP
cord-104279-choywmwd	294	36	,	,	,
cord-104279-choywmwd	294	37	1987	1987	CD
cord-104279-choywmwd	294	38	)	)	-RRB-
cord-104279-choywmwd	294	39	,	,	,
cord-104279-choywmwd	294	40	and	and	CC
cord-104279-choywmwd	294	41	has	have	VBZ
cord-104279-choywmwd	294	42	been	be	VBN
cord-104279-choywmwd	294	43	shown	show	VBN
cord-104279-choywmwd	294	44	by	by	IN
cord-104279-choywmwd	294	45	protease	protease	NN
cord-104279-choywmwd	294	46	treatment	treatment	NN
cord-104279-choywmwd	294	47	of	of	IN
cord-104279-choywmwd	294	48	whole	whole	JJ
cord-104279-choywmwd	294	49	yeast	yeast	NN
cord-104279-choywmwd	294	50	cells	cell	NNS
cord-104279-choywmwd	294	51	to	to	TO
cord-104279-choywmwd	294	52	be	be	VB
cord-104279-choywmwd	294	53	a	a	DT
cord-104279-choywmwd	294	54	plasma	plasma	NN
cord-104279-choywmwd	294	55	membrane	membrane	NN
cord-104279-choywmwd	294	56	protein	protein	NN
cord-104279-choywmwd	294	57	(	(	-LRB-
cord-104279-choywmwd	294	58	Trueheart	Trueheart	NNP
cord-104279-choywmwd	294	59	and	and	CC
cord-104279-choywmwd	294	60	Fink	Fink	NNP
cord-104279-choywmwd	294	61	,	,	,
cord-104279-choywmwd	294	62	1989	1989	CD
cord-104279-choywmwd	294	63	)	)	-RRB-
cord-104279-choywmwd	294	64	.	.	.
cord-104279-choywmwd	295	1	The	the	DT
cord-104279-choywmwd	295	2	fusion	fusion	NN
cord-104279-choywmwd	295	3	protein	protein	NN
cord-104279-choywmwd	295	4	Fusl	Fusl	NNP
cord-104279-choywmwd	295	5	-	-	HYPH
cord-104279-choywmwd	295	6	LacZp	LacZp	NNP
cord-104279-choywmwd	295	7	,	,	,
cord-104279-choywmwd	295	8	consisting	consist	VBG
cord-104279-choywmwd	295	9	of	of	IN
cord-104279-choywmwd	295	10	the	the	DT
cord-104279-choywmwd	295	11	254	254	CD
cord-104279-choywmwd	295	12	NH2-terminal	NH2-terminal	NNP
cord-104279-choywmwd	295	13	amino	amino	NN
cord-104279-choywmwd	295	14	acids	acid	NNS
cord-104279-choywmwd	295	15	of	of	IN
cord-104279-choywmwd	295	16	Fuslp	Fuslp	NNP
cord-104279-choywmwd	295	17	fused	fuse	VBD
cord-104279-choywmwd	295	18	to	to	IN
cord-104279-choywmwd	295	19	the	the	DT
cord-104279-choywmwd	295	20	/	/	SYM
cord-104279-choywmwd	295	21	acZ	acz	JJ
cord-104279-choywmwd	295	22	gene	gene	NN
cord-104279-choywmwd	295	23	product	product	NN
cord-104279-choywmwd	295	24	(	(	-LRB-
cord-104279-choywmwd	295	25	/3-galactosidase	/3-galactosidase	NNP
cord-104279-choywmwd	295	26	)	)	-RRB-
cord-104279-choywmwd	295	27	,	,	,
cord-104279-choywmwd	295	28	has	have	VBZ
cord-104279-choywmwd	295	29	been	be	VBN
cord-104279-choywmwd	295	30	shown	show	VBN
cord-104279-choywmwd	295	31	by	by	IN
cord-104279-choywmwd	295	32	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	295	33	microscopy	microscopy	NN
cord-104279-choywmwd	295	34	to	to	TO
cord-104279-choywmwd	295	35	be	be	VB
cord-104279-choywmwd	295	36	localized	localize	VBN
cord-104279-choywmwd	295	37	to	to	IN
cord-104279-choywmwd	295	38	the	the	DT
cord-104279-choywmwd	295	39	plasma	plasma	NN
cord-104279-choywmwd	295	40	membrane	membrane	NN
cord-104279-choywmwd	295	41	after	after	IN
cord-104279-choywmwd	295	42	induction	induction	NN
cord-104279-choywmwd	295	43	during	during	IN
cord-104279-choywmwd	295	44	mating	mating	NN
cord-104279-choywmwd	295	45	(	(	-LRB-
cord-104279-choywmwd	295	46	Trueheart	Trueheart	NNP
cord-104279-choywmwd	295	47	et	et	NNP
cord-104279-choywmwd	295	48	al	al	NNP
cord-104279-choywmwd	295	49	.	.	NNP
cord-104279-choywmwd	295	50	,	,	,
cord-104279-choywmwd	295	51	1987	1987	CD
cord-104279-choywmwd	295	52	)	)	-RRB-
cord-104279-choywmwd	295	53	.	.	.
cord-104279-choywmwd	296	1	For	for	IN
cord-104279-choywmwd	296	2	these	these	DT
cord-104279-choywmwd	296	3	experiments	experiment	NNS
cord-104279-choywmwd	296	4	,	,	,
cord-104279-choywmwd	296	5	the	the	DT
cord-104279-choywmwd	296	6	expression	expression	NN
cord-104279-choywmwd	296	7	of	of	IN
cord-104279-choywmwd	296	8	these	these	DT
cord-104279-choywmwd	296	9	proteins	protein	NNS
cord-104279-choywmwd	296	10	was	be	VBD
cord-104279-choywmwd	296	11	under	under	IN
cord-104279-choywmwd	296	12	the	the	DT
cord-104279-choywmwd	296	13	control	control	NN
cord-104279-choywmwd	296	14	of	of	IN
cord-104279-choywmwd	296	15	the	the	DT
cord-104279-choywmwd	296	16	inducible	inducible	JJ
cord-104279-choywmwd	296	17	GAL	gal	NN
cord-104279-choywmwd	296	18	/	/	SYM
cord-104279-choywmwd	296	19	promoter	promoter	NN
cord-104279-choywmwd	296	20	(	(	-LRB-
cord-104279-choywmwd	296	21	see	see	VB
cord-104279-choywmwd	296	22	Materials	material	NNS
cord-104279-choywmwd	296	23	and	and	CC
cord-104279-choywmwd	296	24	Methods	Methods	NNPS
cord-104279-choywmwd	296	25	;	;	:
cord-104279-choywmwd	296	26	Johnston	Johnston	NNP
cord-104279-choywmwd	296	27	and	and	CC
cord-104279-choywmwd	296	28	Davis	Davis	NNP
cord-104279-choywmwd	296	29	,	,	,
cord-104279-choywmwd	296	30	1984	1984	CD
cord-104279-choywmwd	296	31	)	)	-RRB-
cord-104279-choywmwd	296	32	.	.	.
cord-104279-choywmwd	297	1	Neither	neither	CC
cord-104279-choywmwd	297	2	Fusl	Fusl	NNP
cord-104279-choywmwd	297	3	-	-	HYPH
cord-104279-choywmwd	297	4	LacZp	LacZp	NNP
cord-104279-choywmwd	297	5	,	,	,
cord-104279-choywmwd	297	6	BB	BB	NNP
cord-104279-choywmwd	297	7	-	-	HYPH
cord-104279-choywmwd	297	8	Inv	Inv	NNP
cord-104279-choywmwd	297	9	,	,	,
cord-104279-choywmwd	297	10	B	b	NN
cord-104279-choywmwd	297	11	-	-	HYPH
cord-104279-choywmwd	297	12	A	A	NNP
cord-104279-choywmwd	297	13	-	-	HYPH
cord-104279-choywmwd	297	14	B	b	NN
cord-104279-choywmwd	297	15	,	,	,
cord-104279-choywmwd	297	16	or	or	CC
cord-104279-choywmwd	297	17	A20-BB	A20-BB	NNP
cord-104279-choywmwd	297	18	showed	show	VBD
cord-104279-choywmwd	297	19	any	any	DT
cord-104279-choywmwd	297	20	signal	signal	NN
cord-104279-choywmwd	297	21	before	before	IN
cord-104279-choywmwd	297	22	addition	addition	NN
cord-104279-choywmwd	297	23	of	of	IN
cord-104279-choywmwd	297	24	galactose	galactose	NN
cord-104279-choywmwd	297	25	(	(	-LRB-
cord-104279-choywmwd	297	26	data	datum	NNS
cord-104279-choywmwd	297	27	not	not	RB
cord-104279-choywmwd	297	28	shown	show	VBN
cord-104279-choywmwd	297	29	)	)	-RRB-
cord-104279-choywmwd	297	30	.	.	.
cord-104279-choywmwd	298	1	Fig	Fig	NNP
cord-104279-choywmwd	298	2	.	.	.
cord-104279-choywmwd	298	3	10	10	CD
cord-104279-choywmwd	299	1	(	(	-LRB-
cord-104279-choywmwd	299	2	A	A	NNP
cord-104279-choywmwd	299	3	-	-	HYPH
cord-104279-choywmwd	299	4	F	f	NN
cord-104279-choywmwd	299	5	)	)	-RRB-
cord-104279-choywmwd	299	6	shows	show	VBZ
cord-104279-choywmwd	299	7	that	that	IN
cord-104279-choywmwd	299	8	Fusl	Fusl	NNP
cord-104279-choywmwd	299	9	-	-	HYPH
cord-104279-choywmwd	299	10	LacZp	LacZp	NNP
cord-104279-choywmwd	299	11	was	be	VBD
cord-104279-choywmwd	299	12	localized	localize	VBN
cord-104279-choywmwd	299	13	to	to	IN
cord-104279-choywmwd	299	14	the	the	DT
cord-104279-choywmwd	299	15	plasma	plasma	NN
cord-104279-choywmwd	299	16	membrane	membrane	NN
cord-104279-choywmwd	299	17	in	in	IN
cord-104279-choywmwd	299	18	a	a	DT
cord-104279-choywmwd	299	19	SEC	SEC	NNP
cord-104279-choywmwd	300	1	§	§	CD
cord-104279-choywmwd	300	2	strain	strain	VBP
cord-104279-choywmwd	300	3	after	after	IN
cord-104279-choywmwd	300	4	2	2	CD
cord-104279-choywmwd	300	5	h	h	NN
cord-104279-choywmwd	300	6	of	of	IN
cord-104279-choywmwd	300	7	induction	induction	NN
cord-104279-choywmwd	300	8	at	at	IN
cord-104279-choywmwd	300	9	34~	34~	CD
cord-104279-choywmwd	300	10	but	but	CC
cord-104279-choywmwd	300	11	accumulated	accumulate	VBN
cord-104279-choywmwd	300	12	intracellularly	intracellularly	RB
cord-104279-choywmwd	300	13	,	,	,
cord-104279-choywmwd	300	14	presumably	presumably	RB
cord-104279-choywmwd	300	15	in	in	IN
cord-104279-choywmwd	300	16	secretory	secretory	JJ
cord-104279-choywmwd	300	17	vesicles	vesicle	NNS
cord-104279-choywmwd	300	18	,	,	,
cord-104279-choywmwd	300	19	in	in	IN
cord-104279-choywmwd	300	20	a	a	DT
cord-104279-choywmwd	300	21	secl	secl	JJ
cord-104279-choywmwd	300	22	strain	strain	NN
cord-104279-choywmwd	300	23	under	under	IN
cord-104279-choywmwd	300	24	the	the	DT
cord-104279-choywmwd	300	25	same	same	JJ
cord-104279-choywmwd	300	26	conditions	condition	NNS
cord-104279-choywmwd	300	27	.	.	.
cord-104279-choywmwd	301	1	No	no	DT
cord-104279-choywmwd	301	2	colocalization	colocalization	NN
cord-104279-choywmwd	301	3	with	with	IN
cord-104279-choywmwd	301	4	the	the	DT
cord-104279-choywmwd	301	5	vacuolar	vacuolar	JJ
cord-104279-choywmwd	301	6	marker	marker	NN
cord-104279-choywmwd	301	7	,	,	,
cord-104279-choywmwd	301	8	ALP	ALP	NNP
cord-104279-choywmwd	301	9	,	,	,
cord-104279-choywmwd	301	10	was	be	VBD
cord-104279-choywmwd	301	11	detected	detect	VBN
cord-104279-choywmwd	301	12	.	.	.
cord-104279-choywmwd	302	1	Figure	figure	NN
cord-104279-choywmwd	302	2	10	10	CD
cord-104279-choywmwd	302	3	.	.	.
cord-104279-choywmwd	303	1	Immunolocalization	immunolocalization	NN
cord-104279-choywmwd	303	2	in	in	IN
cord-104279-choywmwd	303	3	SEC	SEC	NNP
cord-104279-choywmwd	303	4	*	*	NFP
cord-104279-choywmwd	303	5	and	and	CC
cord-104279-choywmwd	303	6	secl	secl	JJ
cord-104279-choywmwd	303	7	strains	strain	NNS
cord-104279-choywmwd	303	8	.	.	.
cord-104279-choywmwd	304	1	JHRY20	JHRY20	NNP
cord-104279-choywmwd	304	2	-	-	HYPH
cord-104279-choywmwd	304	3	1A	1A	NNP
cord-104279-choywmwd	304	4	(	(	-LRB-
cord-104279-choywmwd	304	5	SEC	SEC	NNP
cord-104279-choywmwd	304	6	*	*	NFP
cord-104279-choywmwd	304	7	)	)	-RRB-
cord-104279-choywmwd	304	8	and	and	CC
cord-104279-choywmwd	304	9	SEY-5016	SEY-5016	NNP
cord-104279-choywmwd	304	10	(	(	-LRB-
cord-104279-choywmwd	304	11	secl	secl	NNP
cord-104279-choywmwd	304	12	)	)	-RRB-
cord-104279-choywmwd	304	13	cells	cell	NNS
cord-104279-choywmwd	304	14	containing	contain	VBG
cord-104279-choywmwd	304	15	plasmids	plasmid	NNS
cord-104279-choywmwd	304	16	encoding	encode	VBG
cord-104279-choywmwd	304	17	Fusl	Fusl	NNP
cord-104279-choywmwd	304	18	-	-	HYPH
cord-104279-choywmwd	304	19	LacZp	LacZp	NNP
cord-104279-choywmwd	304	20	(	(	-LRB-
cord-104279-choywmwd	304	21	pCJRll4	pcjrll4	NN
cord-104279-choywmwd	304	22	)	)	-RRB-
cord-104279-choywmwd	304	23	or	or	CC
cord-104279-choywmwd	304	24	B	b	NN
cord-104279-choywmwd	304	25	-	-	HYPH
cord-104279-choywmwd	304	26	A	a	NN
cord-104279-choywmwd	304	27	-	-	HYPH
cord-104279-choywmwd	304	28	B	b	NN
cord-104279-choywmwd	304	29	(	(	-LRB-
cord-104279-choywmwd	304	30	pCJR79	pCJR79	NNP
cord-104279-choywmwd	304	31	)	)	-RRB-
cord-104279-choywmwd	304	32	under	under	IN
cord-104279-choywmwd	304	33	the	the	DT
cord-104279-choywmwd	304	34	control	control	NN
cord-104279-choywmwd	304	35	of	of	IN
cord-104279-choywmwd	304	36	the	the	DT
cord-104279-choywmwd	304	37	GAL/	gal/	JJ
cord-104279-choywmwd	304	38	promoter	promoter	NN
cord-104279-choywmwd	304	39	were	be	VBD
cord-104279-choywmwd	304	40	grown	grow	VBN
cord-104279-choywmwd	304	41	to	to	TO
cord-104279-choywmwd	304	42	log	log	VB
cord-104279-choywmwd	304	43	phase	phase	NN
cord-104279-choywmwd	304	44	in	in	IN
cord-104279-choywmwd	304	45	media	medium	NNS
cord-104279-choywmwd	304	46	containing	contain	VBG
cord-104279-choywmwd	304	47	raffinose	raffinose	NN
cord-104279-choywmwd	304	48	,	,	,
cord-104279-choywmwd	304	49	then	then	RB
cord-104279-choywmwd	304	50	shifted	shift	VBD
cord-104279-choywmwd	304	51	to	to	IN
cord-104279-choywmwd	304	52	34~	34~	CD
cord-104279-choywmwd	304	53	at	at	IN
cord-104279-choywmwd	304	54	the	the	DT
cord-104279-choywmwd	304	55	same	same	JJ
cord-104279-choywmwd	304	56	time	time	NN
cord-104279-choywmwd	304	57	that	that	DT
cord-104279-choywmwd	304	58	galactose	galactose	NN
cord-104279-choywmwd	304	59	was	be	VBD
cord-104279-choywmwd	304	60	added	add	VBN
cord-104279-choywmwd	304	61	to	to	IN
cord-104279-choywmwd	304	62	the	the	DT
cord-104279-choywmwd	304	63	cultures	culture	NNS
cord-104279-choywmwd	304	64	.	.	.
cord-104279-choywmwd	305	1	After	after	IN
cord-104279-choywmwd	305	2	2	2	CD
cord-104279-choywmwd	305	3	h	h	NN
cord-104279-choywmwd	305	4	,	,	,
cord-104279-choywmwd	305	5	the	the	DT
cord-104279-choywmwd	305	6	cells	cell	NNS
cord-104279-choywmwd	305	7	were	be	VBD
cord-104279-choywmwd	305	8	fixed	fix	VBN
cord-104279-choywmwd	305	9	,	,	,
cord-104279-choywmwd	305	10	spheroplasted	spheroplasted	JJ
cord-104279-choywmwd	305	11	,	,	,
cord-104279-choywmwd	305	12	and	and	CC
cord-104279-choywmwd	305	13	prepared	prepare	VBN
cord-104279-choywmwd	305	14	for	for	IN
cord-104279-choywmwd	305	15	immunofluorescence	immunofluorescence	NN
cord-104279-choywmwd	305	16	.	.	.
cord-104279-choywmwd	306	1	Cells	cell	NNS
cord-104279-choywmwd	306	2	expressing	express	VBG
cord-104279-choywmwd	306	3	Fusl	Fusl	NNP
cord-104279-choywmwd	306	4	-	-	HYPH
cord-104279-choywmwd	306	5	LacZp	LacZp	NNP
cord-104279-choywmwd	306	6	were	be	VBD
cord-104279-choywmwd	306	7	stained	stain	VBN
cord-104279-choywmwd	306	8	with	with	IN
cord-104279-choywmwd	307	1	anti-/~-galactosidase	anti-/~-galactosidase	JJ
cord-104279-choywmwd	307	2	mAb	mAb	NNS
cord-104279-choywmwd	307	3	and	and	CC
cord-104279-choywmwd	307	4	anti	anti	JJ
cord-104279-choywmwd	307	5	-	-	JJ
cord-104279-choywmwd	307	6	ALP	alp	JJ
cord-104279-choywmwd	307	7	polyclonal	polyclonal	JJ
cord-104279-choywmwd	307	8	antibody	antibody	NN
cord-104279-choywmwd	307	9	,	,	,
cord-104279-choywmwd	307	10	and	and	CC
cord-104279-choywmwd	307	11	were	be	VBD
cord-104279-choywmwd	307	12	viewed	view	VBN
cord-104279-choywmwd	307	13	using	use	VBG
cord-104279-choywmwd	307	14	filter	filter	NN
cord-104279-choywmwd	307	15	sets	set	NNS
cord-104279-choywmwd	307	16	specific	specific	JJ
cord-104279-choywmwd	307	17	for	for	IN
cord-104279-choywmwd	307	18	fluorescein	fluorescein	NN
cord-104279-choywmwd	307	19	and	and	CC
cord-104279-choywmwd	307	20	rhodamine	rhodamine	NN
cord-104279-choywmwd	307	21	fluorescence	fluorescence	NN
cord-104279-choywmwd	307	22	,	,	,
cord-104279-choywmwd	307	23	respectively	respectively	RB
cord-104279-choywmwd	307	24	.	.	.
cord-104279-choywmwd	308	1	Cells	cell	NNS
cord-104279-choywmwd	308	2	expressing	express	VBG
cord-104279-choywmwd	308	3	B	b	NN
cord-104279-choywmwd	308	4	-	-	HYPH
cord-104279-choywmwd	308	5	A	a	NN
cord-104279-choywmwd	308	6	-	-	HYPH
cord-104279-choywmwd	308	7	B	b	NN
cord-104279-choywmwd	308	8	were	be	VBD
cord-104279-choywmwd	308	9	stained	stain	VBN
cord-104279-choywmwd	308	10	as	as	IN
cord-104279-choywmwd	308	11	in	in	IN
cord-104279-choywmwd	308	12	Fig	Fig	NNP
cord-104279-choywmwd	308	13	.	.	.
cord-104279-choywmwd	309	1	5	5	LS
cord-104279-choywmwd	309	2	.	.	.
cord-104279-choywmwd	310	1	Typically	typically	RB
cord-104279-choywmwd	310	2	,	,	,
cord-104279-choywmwd	310	3	Fusl	Fusl	NNP
cord-104279-choywmwd	310	4	-	-	HYPH
cord-104279-choywmwd	310	5	LacZp	LacZp	NNP
cord-104279-choywmwd	310	6	accumulated	accumulate	VBN
cord-104279-choywmwd	310	7	in	in	IN
cord-104279-choywmwd	310	8	a	a	DT
cord-104279-choywmwd	310	9	large	large	JJ
cord-104279-choywmwd	310	10	patch	patch	NN
cord-104279-choywmwd	310	11	in	in	IN
cord-104279-choywmwd	310	12	the	the	DT
cord-104279-choywmwd	310	13	bud	bud	NN
cord-104279-choywmwd	310	14	and	and	CC
cord-104279-choywmwd	310	15	bud	bud	NN
cord-104279-choywmwd	310	16	neck	neck	NN
cord-104279-choywmwd	310	17	region	region	NN
cord-104279-choywmwd	310	18	of	of	IN
cord-104279-choywmwd	310	19	budded	bud	VBN
cord-104279-choywmwd	310	20	cells	cell	NNS
cord-104279-choywmwd	310	21	,	,	,
cord-104279-choywmwd	310	22	and	and	CC
cord-104279-choywmwd	310	23	near	near	IN
cord-104279-choywmwd	310	24	the	the	DT
cord-104279-choywmwd	310	25	cell	cell	NN
cord-104279-choywmwd	310	26	periphery	periphery	NN
cord-104279-choywmwd	310	27	of	of	IN
cord-104279-choywmwd	310	28	unbudded	unbudded	JJ
cord-104279-choywmwd	310	29	cells	cell	NNS
cord-104279-choywmwd	310	30	.	.	.
cord-104279-choywmwd	311	1	BB	BB	NNP
cord-104279-choywmwd	311	2	-	-	HYPH
cord-104279-choywmwd	311	3	Inv	Inv	NNP
cord-104279-choywmwd	311	4	,	,	,
cord-104279-choywmwd	311	5	B	b	NN
cord-104279-choywmwd	311	6	-	-	HYPH
cord-104279-choywmwd	311	7	A	A	NNP
cord-104279-choywmwd	311	8	-	-	HYPH
cord-104279-choywmwd	311	9	B	b	NN
cord-104279-choywmwd	311	10	,	,	,
cord-104279-choywmwd	311	11	and	and	CC
cord-104279-choywmwd	311	12	A20-BB	a20-bb	CD
cord-104279-choywmwd	311	13	were	be	VBD
cord-104279-choywmwd	311	14	each	each	DT
cord-104279-choywmwd	311	15	localized	localized	JJ
cord-104279-choywmwd	311	16	to	to	IN
cord-104279-choywmwd	311	17	the	the	DT
cord-104279-choywmwd	311	18	vacuole	vacuole	NN
cord-104279-choywmwd	311	19	under	under	IN
cord-104279-choywmwd	311	20	these	these	DT
cord-104279-choywmwd	311	21	conditions	condition	NNS
cord-104279-choywmwd	311	22	(	(	-LRB-
cord-104279-choywmwd	311	23	e.g.	e.g.	RB
cord-104279-choywmwd	311	24	,	,	,
cord-104279-choywmwd	311	25	Fig	Fig	NNP
cord-104279-choywmwd	311	26	.	.	NNP
cord-104279-choywmwd	311	27	10	10	CD
cord-104279-choywmwd	311	28	,	,	,
cord-104279-choywmwd	311	29	G	G	NNP
cord-104279-choywmwd	311	30	-	-	HYPH
cord-104279-choywmwd	311	31	l	l	NN
cord-104279-choywmwd	311	32	;	;	:
cord-104279-choywmwd	311	33	Table	Table	NNP
cord-104279-choywmwd	311	34	II	II	NNP
cord-104279-choywmwd	311	35	)	)	-RRB-
cord-104279-choywmwd	311	36	,	,	,
cord-104279-choywmwd	311	37	as	as	IN
cord-104279-choywmwd	311	38	defined	define	VBN
cord-104279-choywmwd	311	39	by	by	IN
cord-104279-choywmwd	311	40	co	co	NN
cord-104279-choywmwd	311	41	-	-	NN
cord-104279-choywmwd	311	42	localization	localization	NN
cord-104279-choywmwd	311	43	with	with	IN
cord-104279-choywmwd	311	44	Vat2p	Vat2p	NNP
cord-104279-choywmwd	311	45	.	.	.
cord-104279-choywmwd	312	1	The	the	DT
cord-104279-choywmwd	312	2	intraceUular	intraceUular	NNP
cord-104279-choywmwd	312	3	,	,	,
cord-104279-choywmwd	312	4	non	non	JJ
cord-104279-choywmwd	312	5	-	-	JJ
cord-104279-choywmwd	312	6	vacuolar	vacuolar	JJ
cord-104279-choywmwd	312	7	signal	signal	NN
cord-104279-choywmwd	312	8	occasionally	occasionally	RB
cord-104279-choywmwd	312	9	observed	observe	VBD
cord-104279-choywmwd	312	10	for	for	IN
cord-104279-choywmwd	312	11	B	b	NN
cord-104279-choywmwd	312	12	-	-	HYPH
cord-104279-choywmwd	312	13	A	a	NN
cord-104279-choywmwd	312	14	-	-	HYPH
cord-104279-choywmwd	312	15	B	b	NN
cord-104279-choywmwd	312	16	(	(	-LRB-
cord-104279-choywmwd	312	17	23	23	CD
cord-104279-choywmwd	312	18	%	%	NN
cord-104279-choywmwd	312	19	;	;	:
cord-104279-choywmwd	312	20	Table	Table	NNP
cord-104279-choywmwd	312	21	II	II	NNP
cord-104279-choywmwd	312	22	)	)	-RRB-
cord-104279-choywmwd	312	23	was	be	VBD
cord-104279-choywmwd	312	24	different	different	JJ
cord-104279-choywmwd	312	25	from	from	IN
cord-104279-choywmwd	312	26	that	that	DT
cord-104279-choywmwd	312	27	of	of	IN
cord-104279-choywmwd	312	28	Fusl	Fusl	NNP
cord-104279-choywmwd	312	29	-	-	HYPH
cord-104279-choywmwd	312	30	LacZp	LacZp	NNP
cord-104279-choywmwd	312	31	,	,	,
cord-104279-choywmwd	312	32	in	in	IN
cord-104279-choywmwd	312	33	that	that	IN
cord-104279-choywmwd	312	34	the	the	DT
cord-104279-choywmwd	312	35	signal	signal	NN
cord-104279-choywmwd	312	36	looked	look	VBD
cord-104279-choywmwd	312	37	reminiscent	reminiscent	JJ
cord-104279-choywmwd	312	38	of	of	IN
cord-104279-choywmwd	312	39	the	the	DT
cord-104279-choywmwd	312	40	Golgi	Golgi	NNP
cord-104279-choywmwd	312	41	signal	signal	NN
cord-104279-choywmwd	312	42	observed	observe	VBN
cord-104279-choywmwd	312	43	for	for	IN
cord-104279-choywmwd	312	44	DPAP	DPAP	NNP
cord-104279-choywmwd	312	45	A	A	NNP
cord-104279-choywmwd	312	46	(	(	-LRB-
cord-104279-choywmwd	312	47	data	data	NN
cord-104279-choywmwd	312	48	not	not	RB
cord-104279-choywmwd	312	49	shown	show	VBN
cord-104279-choywmwd	312	50	)	)	-RRB-
cord-104279-choywmwd	312	51	.	.	.
cord-104279-choywmwd	313	1	This	this	DT
cord-104279-choywmwd	313	2	was	be	VBD
cord-104279-choywmwd	313	3	also	also	RB
cord-104279-choywmwd	313	4	observed	observe	VBN
cord-104279-choywmwd	313	5	for	for	IN
cord-104279-choywmwd	313	6	B	b	NN
cord-104279-choywmwd	313	7	-	-	HYPH
cord-104279-choywmwd	313	8	A	A	NNP
cord-104279-choywmwd	313	9	-	-	HYPH
cord-104279-choywmwd	313	10	B	b	NN
cord-104279-choywmwd	313	11	in	in	IN
cord-104279-choywmwd	313	12	SEC	SEC	NNP
cord-104279-choywmwd	313	13	§	§	:
cord-104279-choywmwd	313	14	cells	cell	NNS
cord-104279-choywmwd	313	15	at	at	IN
cord-104279-choywmwd	313	16	34~	34~	CD
cord-104279-choywmwd	313	17	suggesting	suggest	VBG
cord-104279-choywmwd	313	18	that	that	IN
cord-104279-choywmwd	313	19	the	the	DT
cord-104279-choywmwd	313	20	kinetics	kinetic	NNS
cord-104279-choywmwd	313	21	of	of	IN
cord-104279-choywmwd	313	22	delivery	delivery	NN
cord-104279-choywmwd	313	23	of	of	IN
cord-104279-choywmwd	313	24	this	this	DT
cord-104279-choywmwd	313	25	protein	protein	NN
cord-104279-choywmwd	313	26	is	be	VBZ
cord-104279-choywmwd	313	27	slowed	slow	VBN
cord-104279-choywmwd	313	28	at	at	IN
cord-104279-choywmwd	313	29	elevated	elevated	JJ
cord-104279-choywmwd	313	30	temperatures	temperature	NNS
cord-104279-choywmwd	313	31	.	.	.
cord-104279-choywmwd	314	1	The	the	DT
cord-104279-choywmwd	314	2	analysis	analysis	NN
cord-104279-choywmwd	314	3	of	of	IN
cord-104279-choywmwd	314	4	A22-AA	a22-aa	NN
cord-104279-choywmwd	314	5	-	-	HYPH
cord-104279-choywmwd	314	6	B	B	NNP
cord-104279-choywmwd	314	7	in	in	IN
cord-104279-choywmwd	314	8	the	the	DT
cord-104279-choywmwd	314	9	secl	secl	NNP
cord-104279-choywmwd	314	10	strain	strain	NN
cord-104279-choywmwd	314	11	was	be	VBD
cord-104279-choywmwd	314	12	complicated	complicate	VBN
cord-104279-choywmwd	314	13	by	by	IN
cord-104279-choywmwd	314	14	the	the	DT
cord-104279-choywmwd	314	15	fact	fact	NN
cord-104279-choywmwd	314	16	that	that	IN
cord-104279-choywmwd	314	17	expression	expression	NN
cord-104279-choywmwd	314	18	of	of	IN
cord-104279-choywmwd	314	19	the	the	DT
cord-104279-choywmwd	314	20	protein	protein	NN
cord-104279-choywmwd	314	21	was	be	VBD
cord-104279-choywmwd	314	22	leaky	leaky	JJ
cord-104279-choywmwd	314	23	;	;	:
cord-104279-choywmwd	314	24	,	,	,
cord-104279-choywmwd	314	25	~50	~50	NFP
cord-104279-choywmwd	314	26	%	%	NN
cord-104279-choywmwd	314	27	of	of	IN
cord-104279-choywmwd	314	28	the	the	DT
cord-104279-choywmwd	314	29	cells	cell	NNS
cord-104279-choywmwd	314	30	in	in	IN
cord-104279-choywmwd	314	31	the	the	DT
cord-104279-choywmwd	314	32	population	population	NN
cord-104279-choywmwd	314	33	showed	show	VBD
cord-104279-choywmwd	314	34	a	a	DT
cord-104279-choywmwd	314	35	weak	weak	JJ
cord-104279-choywmwd	314	36	vacuolar	vacuolar	JJ
cord-104279-choywmwd	314	37	staining	staining	NN
cord-104279-choywmwd	314	38	pattern	pattern	NN
cord-104279-choywmwd	314	39	before	before	IN
cord-104279-choywmwd	314	40	galactose	galactose	NN
cord-104279-choywmwd	314	41	addition	addition	NN
cord-104279-choywmwd	314	42	.	.	.
cord-104279-choywmwd	315	1	However	however	RB
cord-104279-choywmwd	315	2	,	,	,
cord-104279-choywmwd	315	3	addition	addition	NN
cord-104279-choywmwd	315	4	of	of	IN
cord-104279-choywmwd	315	5	galactose	galactose	NN
cord-104279-choywmwd	315	6	to	to	IN
cord-104279-choywmwd	315	7	the	the	DT
cord-104279-choywmwd	315	8	culture	culture	NN
cord-104279-choywmwd	315	9	after	after	IN
cord-104279-choywmwd	315	10	shifting	shift	VBG
cord-104279-choywmwd	315	11	the	the	DT
cord-104279-choywmwd	315	12	cells	cell	NNS
cord-104279-choywmwd	315	13	to	to	IN
cord-104279-choywmwd	315	14	34~	34~	CD
cord-104279-choywmwd	315	15	resulted	result	VBD
cord-104279-choywmwd	315	16	in	in	IN
cord-104279-choywmwd	315	17	'	'	``
cord-104279-choywmwd	315	18	~90	~90	CD
cord-104279-choywmwd	315	19	%	%	NN
cord-104279-choywmwd	315	20	of	of	IN
cord-104279-choywmwd	315	21	the	the	DT
cord-104279-choywmwd	315	22	cells	cell	NNS
cord-104279-choywmwd	315	23	exhibiting	exhibit	VBG
cord-104279-choywmwd	315	24	an	an	DT
cord-104279-choywmwd	315	25	intense	intense	JJ
cord-104279-choywmwd	315	26	vacuolar	vacuolar	JJ
cord-104279-choywmwd	315	27	signal	signal	NN
cord-104279-choywmwd	315	28	(	(	-LRB-
cord-104279-choywmwd	315	29	data	datum	NNS
cord-104279-choywmwd	315	30	not	not	RB
cord-104279-choywmwd	315	31	shown	show	VBN
cord-104279-choywmwd	315	32	)	)	-RRB-
cord-104279-choywmwd	315	33	.	.	.
cord-104279-choywmwd	316	1	These	these	DT
cord-104279-choywmwd	316	2	data	datum	NNS
cord-104279-choywmwd	316	3	show	show	VBP
cord-104279-choywmwd	316	4	that	that	IN
cord-104279-choywmwd	316	5	the	the	DT
cord-104279-choywmwd	316	6	BB	BB	NNP
cord-104279-choywmwd	316	7	-	-	HYPH
cord-104279-choywmwd	316	8	Inv	Inv	NNP
cord-104279-choywmwd	316	9	,	,	,
cord-104279-choywmwd	316	10	B	b	NN
cord-104279-choywmwd	316	11	-	-	HYPH
cord-104279-choywmwd	316	12	A	A	NNP
cord-104279-choywmwd	316	13	-	-	HYPH
cord-104279-choywmwd	316	14	B	b	NN
cord-104279-choywmwd	316	15	,	,	,
cord-104279-choywmwd	316	16	A20-BB	A20-BB	NNP
cord-104279-choywmwd	316	17	,	,	,
cord-104279-choywmwd	316	18	and	and	CC
cord-104279-choywmwd	316	19	A22-AA	a22-aa	NN
cord-104279-choywmwd	316	20	-	-	HYPH
cord-104279-choywmwd	316	21	B	b	NN
cord-104279-choywmwd	316	22	proteins	protein	NNS
cord-104279-choywmwd	316	23	are	be	VBP
cord-104279-choywmwd	316	24	targeted	target	VBN
cord-104279-choywmwd	316	25	directly	directly	RB
cord-104279-choywmwd	316	26	from	from	IN
cord-104279-choywmwd	316	27	the	the	DT
cord-104279-choywmwd	316	28	Golgi	Golgi	NNP
cord-104279-choywmwd	316	29	apparatus	apparatus	NN
cord-104279-choywmwd	316	30	to	to	IN
cord-104279-choywmwd	316	31	the	the	DT
cord-104279-choywmwd	316	32	vacuole	vacuole	NN
cord-104279-choywmwd	316	33	without	without	IN
cord-104279-choywmwd	316	34	transient	transient	JJ
cord-104279-choywmwd	316	35	appearance	appearance	NN
cord-104279-choywmwd	316	36	at	at	IN
cord-104279-choywmwd	316	37	the	the	DT
cord-104279-choywmwd	316	38	cell	cell	NN
cord-104279-choywmwd	316	39	surface	surface	NN
cord-104279-choywmwd	316	40	;	;	:
cord-104279-choywmwd	316	41	thus	thus	RB
cord-104279-choywmwd	316	42	,	,	,
cord-104279-choywmwd	316	43	these	these	DT
cord-104279-choywmwd	316	44	proteins	protein	NNS
cord-104279-choywmwd	316	45	follow	follow	VBP
cord-104279-choywmwd	316	46	the	the	DT
cord-104279-choywmwd	316	47	same	same	JJ
cord-104279-choywmwd	316	48	route	route	NN
cord-104279-choywmwd	316	49	to	to	IN
cord-104279-choywmwd	316	50	the	the	DT
cord-104279-choywmwd	316	51	vacuole	vacuole	NN
cord-104279-choywmwd	316	52	as	as	IN
cord-104279-choywmwd	316	53	wild	wild	JJ
cord-104279-choywmwd	316	54	type	type	NN
cord-104279-choywmwd	316	55	vacuolar	vacuolar	NNP
cord-104279-choywmwd	316	56	membrane	membrane	NN
cord-104279-choywmwd	316	57	proteins	protein	NNS
cord-104279-choywmwd	316	58	(	(	-LRB-
cord-104279-choywmwd	316	59	Roberts	Roberts	NNP
cord-104279-choywmwd	316	60	et	et	NNP
cord-104279-choywmwd	316	61	al	al	NNP
cord-104279-choywmwd	316	62	.	.	NNP
cord-104279-choywmwd	316	63	,	,	,
cord-104279-choywmwd	316	64	1989	1989	CD
cord-104279-choywmwd	316	65	)	)	-RRB-
cord-104279-choywmwd	316	66	.	.	.
cord-104279-choywmwd	317	1	The	the	DT
cord-104279-choywmwd	317	2	search	search	NN
cord-104279-choywmwd	317	3	for	for	IN
cord-104279-choywmwd	317	4	the	the	DT
cord-104279-choywmwd	317	5	vacuolar	vacuolar	NNP
cord-104279-choywmwd	317	6	localization	localization	NN
cord-104279-choywmwd	317	7	determinant	determinant	NN
cord-104279-choywmwd	317	8	of	of	IN
cord-104279-choywmwd	317	9	DPAP	DPAP	NNP
cord-104279-choywmwd	317	10	B	B	NNP
cord-104279-choywmwd	317	11	led	lead	VBD
cord-104279-choywmwd	317	12	to	to	IN
cord-104279-choywmwd	317	13	the	the	DT
cord-104279-choywmwd	317	14	surprising	surprising	JJ
cord-104279-choywmwd	317	15	conclusion	conclusion	NN
cord-104279-choywmwd	317	16	that	that	IN
cord-104279-choywmwd	317	17	neither	neither	CC
cord-104279-choywmwd	317	18	the	the	DT
cord-104279-choywmwd	317	19	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	317	20	,	,	,
cord-104279-choywmwd	317	21	transmembrane	transmembrane	NN
cord-104279-choywmwd	317	22	,	,	,
cord-104279-choywmwd	317	23	nor	nor	CC
cord-104279-choywmwd	317	24	lumenal	lumenal	JJ
cord-104279-choywmwd	317	25	domain	domain	NN
cord-104279-choywmwd	317	26	of	of	IN
cord-104279-choywmwd	317	27	the	the	DT
cord-104279-choywmwd	317	28	protein	protein	NN
cord-104279-choywmwd	317	29	was	be	VBD
cord-104279-choywmwd	317	30	necessary	necessary	JJ
cord-104279-choywmwd	317	31	for	for	IN
cord-104279-choywmwd	317	32	vacuolar	vacuolar	JJ
cord-104279-choywmwd	317	33	delivery	delivery	NN
cord-104279-choywmwd	317	34	.	.	.
cord-104279-choywmwd	318	1	Analysis	analysis	NN
cord-104279-choywmwd	318	2	of	of	IN
cord-104279-choywmwd	318	3	the	the	DT
cord-104279-choywmwd	318	4	Golgi	Golgi	NNP
cord-104279-choywmwd	318	5	retention	retention	NN
cord-104279-choywmwd	318	6	signal	signal	NN
cord-104279-choywmwd	318	7	of	of	IN
cord-104279-choywmwd	318	8	DPAP	DPAP	NNP
cord-104279-choywmwd	318	9	A	A	NNP
cord-104279-choywmwd	318	10	led	lead	VBD
cord-104279-choywmwd	318	11	to	to	IN
cord-104279-choywmwd	318	12	the	the	DT
cord-104279-choywmwd	318	13	equally	equally	RB
cord-104279-choywmwd	318	14	surprising	surprising	JJ
cord-104279-choywmwd	318	15	observations	observation	NNS
cord-104279-choywmwd	318	16	that	that	WDT
cord-104279-choywmwd	318	17	both	both	DT
cord-104279-choywmwd	318	18	overproduction	overproduction	NN
cord-104279-choywmwd	318	19	of	of	IN
cord-104279-choywmwd	318	20	the	the	DT
cord-104279-choywmwd	318	21	protein	protein	NN
cord-104279-choywmwd	318	22	and	and	CC
cord-104279-choywmwd	318	23	mutations	mutation	NNS
cord-104279-choywmwd	318	24	in	in	IN
cord-104279-choywmwd	318	25	the	the	DT
cord-104279-choywmwd	318	26	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	318	27	domain	domain	NN
cord-104279-choywmwd	318	28	resulted	result	VBD
cord-104279-choywmwd	318	29	in	in	IN
cord-104279-choywmwd	318	30	mislocalization	mislocalization	NN
cord-104279-choywmwd	318	31	of	of	IN
cord-104279-choywmwd	318	32	DPAP	DPAP	NNP
cord-104279-choywmwd	318	33	A	A	NNP
cord-104279-choywmwd	318	34	to	to	IN
cord-104279-choywmwd	318	35	the	the	DT
cord-104279-choywmwd	318	36	vacuole	vacuole	NN
cord-104279-choywmwd	318	37	,	,	,
cord-104279-choywmwd	318	38	not	not	RB
cord-104279-choywmwd	318	39	the	the	DT
cord-104279-choywmwd	318	40	plasma	plasma	NN
cord-104279-choywmwd	318	41	membrane	membrane	NN
cord-104279-choywmwd	318	42	.	.	.
cord-104279-choywmwd	319	1	These	these	DT
cord-104279-choywmwd	319	2	results	result	NNS
cord-104279-choywmwd	319	3	were	be	VBD
cord-104279-choywmwd	319	4	unexpected	unexpected	JJ
cord-104279-choywmwd	319	5	,	,	,
cord-104279-choywmwd	319	6	given	give	VBN
cord-104279-choywmwd	319	7	that	that	IN
cord-104279-choywmwd	319	8	the	the	DT
cord-104279-choywmwd	319	9	targeting	targeting	NN
cord-104279-choywmwd	319	10	of	of	IN
cord-104279-choywmwd	319	11	soluble	soluble	JJ
cord-104279-choywmwd	319	12	vacuolar	vacuolar	JJ
cord-104279-choywmwd	319	13	proteins	protein	NNS
cord-104279-choywmwd	319	14	in	in	IN
cord-104279-choywmwd	319	15	yeast	yeast	NN
cord-104279-choywmwd	319	16	and	and	CC
cord-104279-choywmwd	319	17	soluble	soluble	JJ
cord-104279-choywmwd	319	18	and	and	CC
cord-104279-choywmwd	319	19	membrane	membrane	NN
cord-104279-choywmwd	319	20	proteins	protein	NNS
cord-104279-choywmwd	319	21	of	of	IN
cord-104279-choywmwd	319	22	the	the	DT
cord-104279-choywmwd	319	23	lysosomes	lysosome	NNS
cord-104279-choywmwd	319	24	of	of	IN
cord-104279-choywmwd	319	25	animal	animal	NN
cord-104279-choywmwd	319	26	cells	cell	NNS
cord-104279-choywmwd	319	27	require	require	VBP
cord-104279-choywmwd	319	28	targeting	target	VBG
cord-104279-choywmwd	319	29	information	information	NN
cord-104279-choywmwd	319	30	to	to	TO
cord-104279-choywmwd	319	31	prevent	prevent	VB
cord-104279-choywmwd	319	32	delivery	delivery	NN
cord-104279-choywmwd	319	33	to	to	IN
cord-104279-choywmwd	319	34	the	the	DT
cord-104279-choywmwd	319	35	cell	cell	NN
cord-104279-choywmwd	319	36	surface	surface	NN
cord-104279-choywmwd	319	37	(	(	-LRB-
cord-104279-choywmwd	319	38	Vails	Vails	NNP
cord-104279-choywmwd	319	39	et	et	NNP
cord-104279-choywmwd	319	40	al	al	NNP
cord-104279-choywmwd	319	41	.	.	NNP
cord-104279-choywmwd	319	42	,	,	,
cord-104279-choywmwd	319	43	1990	1990	CD
cord-104279-choywmwd	319	44	;	;	:
cord-104279-choywmwd	319	45	Kornfeld	Kornfeld	NNP
cord-104279-choywmwd	319	46	and	and	CC
cord-104279-choywmwd	319	47	Mellman	Mellman	NNP
cord-104279-choywmwd	319	48	,	,	,
cord-104279-choywmwd	319	49	1989	1989	CD
cord-104279-choywmwd	319	50	;	;	:
cord-104279-choywmwd	319	51	Williams	Williams	NNP
cord-104279-choywmwd	319	52	and	and	CC
cord-104279-choywmwd	319	53	Fukuda	Fukuda	NNP
cord-104279-choywmwd	319	54	,	,	,
cord-104279-choywmwd	319	55	1990	1990	CD
cord-104279-choywmwd	319	56	;	;	:
cord-104279-choywmwd	319	57	Machamer	Machamer	NNP
cord-104279-choywmwd	319	58	,	,	,
cord-104279-choywmwd	319	59	1991	1991	CD
cord-104279-choywmwd	319	60	)	)	-RRB-
cord-104279-choywmwd	319	61	.	.	.
cord-104279-choywmwd	320	1	The	the	DT
cord-104279-choywmwd	320	2	fusion	fusion	NN
cord-104279-choywmwd	320	3	proteins	protein	NNS
cord-104279-choywmwd	320	4	analyzed	analyze	VBN
cord-104279-choywmwd	320	5	in	in	IN
cord-104279-choywmwd	320	6	this	this	DT
cord-104279-choywmwd	320	7	study	study	NN
cord-104279-choywmwd	320	8	are	be	VBP
cord-104279-choywmwd	320	9	appropriate	appropriate	JJ
cord-104279-choywmwd	320	10	tools	tool	NNS
cord-104279-choywmwd	320	11	for	for	IN
cord-104279-choywmwd	320	12	these	these	DT
cord-104279-choywmwd	320	13	experiments	experiment	NNS
cord-104279-choywmwd	320	14	for	for	IN
cord-104279-choywmwd	320	15	the	the	DT
cord-104279-choywmwd	320	16	following	follow	VBG
cord-104279-choywmwd	320	17	reasons	reason	NNS
cord-104279-choywmwd	320	18	.	.	.
cord-104279-choywmwd	321	1	All	all	DT
cord-104279-choywmwd	321	2	of	of	IN
cord-104279-choywmwd	321	3	the	the	DT
cord-104279-choywmwd	321	4	proteins	protein	NNS
cord-104279-choywmwd	321	5	were	be	VBD
cord-104279-choywmwd	321	6	stable	stable	JJ
cord-104279-choywmwd	321	7	,	,	,
cord-104279-choywmwd	321	8	enzymatically	enzymatically	RB
cord-104279-choywmwd	321	9	active	active	JJ
cord-104279-choywmwd	321	10	,	,	,
cord-104279-choywmwd	321	11	membranebound	membranebound	JJ
cord-104279-choywmwd	321	12	,	,	,
cord-104279-choywmwd	321	13	and	and	CC
cord-104279-choywmwd	321	14	glycosylated	glycosylate	VBN
cord-104279-choywmwd	321	15	when	when	WRB
cord-104279-choywmwd	321	16	expressed	express	VBN
cord-104279-choywmwd	321	17	in	in	IN
cord-104279-choywmwd	321	18	yeast	yeast	NN
cord-104279-choywmwd	321	19	,	,	,
cord-104279-choywmwd	321	20	indicating	indicate	VBG
cord-104279-choywmwd	321	21	that	that	IN
cord-104279-choywmwd	321	22	they	-PRON-	PRP
cord-104279-choywmwd	321	23	have	have	VBP
cord-104279-choywmwd	321	24	the	the	DT
cord-104279-choywmwd	321	25	correct	correct	JJ
cord-104279-choywmwd	321	26	membrane	membrane	NN
cord-104279-choywmwd	321	27	topology	topology	NN
cord-104279-choywmwd	321	28	.	.	.
cord-104279-choywmwd	322	1	In	in	IN
cord-104279-choywmwd	322	2	addition	addition	NN
cord-104279-choywmwd	322	3	,	,	,
cord-104279-choywmwd	322	4	all	all	DT
cord-104279-choywmwd	322	5	of	of	IN
cord-104279-choywmwd	322	6	the	the	DT
cord-104279-choywmwd	322	7	fusion	fusion	NN
cord-104279-choywmwd	322	8	proteins	protein	NNS
cord-104279-choywmwd	322	9	containing	contain	VBG
cord-104279-choywmwd	322	10	the	the	DT
cord-104279-choywmwd	322	11	DPAP	DPAP	NNP
cord-104279-choywmwd	322	12	B	B	NNP
cord-104279-choywmwd	322	13	lumenal	lumenal	JJ
cord-104279-choywmwd	322	14	domain	domain	NN
cord-104279-choywmwd	322	15	,	,	,
cord-104279-choywmwd	322	16	i.e.	i.e.	FW
cord-104279-choywmwd	322	17	,	,	,
cord-104279-choywmwd	322	18	A20-BB	A20-BB	NNP
cord-104279-choywmwd	322	19	,	,	,
cord-104279-choywmwd	322	20	A27-BB	A27-BB	NNP
cord-104279-choywmwd	322	21	,	,	,
cord-104279-choywmwd	322	22	B	b	NN
cord-104279-choywmwd	322	23	-	-	HYPH
cord-104279-choywmwd	322	24	A	A	NNP
cord-104279-choywmwd	322	25	-	-	HYPH
cord-104279-choywmwd	322	26	B	b	NN
cord-104279-choywmwd	322	27	,	,	,
cord-104279-choywmwd	322	28	and	and	CC
cord-104279-choywmwd	322	29	A22-AA	a22-aa	NN
cord-104279-choywmwd	322	30	-	-	HYPH
cord-104279-choywmwd	322	31	B	B	NNP
cord-104279-choywmwd	322	32	,	,	,
cord-104279-choywmwd	322	33	were	be	VBD
cord-104279-choywmwd	322	34	transported	transport	VBN
cord-104279-choywmwd	322	35	to	to	IN
cord-104279-choywmwd	322	36	the	the	DT
cord-104279-choywmwd	322	37	vacuole	vacuole	NN
cord-104279-choywmwd	322	38	after	after	IN
cord-104279-choywmwd	322	39	receiving	receive	VBG
cord-104279-choywmwd	322	40	glycosyl	glycosyl	JJ
cord-104279-choywmwd	322	41	modifications	modification	NNS
cord-104279-choywmwd	322	42	in	in	IN
cord-104279-choywmwd	322	43	the	the	DT
cord-104279-choywmwd	322	44	Golgi	Golgi	NNP
cord-104279-choywmwd	322	45	apparatus	apparatus	NN
cord-104279-choywmwd	322	46	.	.	.
cord-104279-choywmwd	323	1	Finally	finally	RB
cord-104279-choywmwd	323	2	,	,	,
cord-104279-choywmwd	323	3	each	each	DT
cord-104279-choywmwd	323	4	of	of	IN
cord-104279-choywmwd	323	5	these	these	DT
cord-104279-choywmwd	323	6	fusion	fusion	NN
cord-104279-choywmwd	323	7	proteins	protein	NNS
cord-104279-choywmwd	323	8	was	be	VBD
cord-104279-choywmwd	323	9	transported	transport	VBN
cord-104279-choywmwd	323	10	to	to	IN
cord-104279-choywmwd	323	11	the	the	DT
cord-104279-choywmwd	323	12	vacuole	vacuole	NN
cord-104279-choywmwd	323	13	directly	directly	RB
cord-104279-choywmwd	323	14	from	from	IN
cord-104279-choywmwd	323	15	the	the	DT
cord-104279-choywmwd	323	16	Golgi	Golgi	NNP
cord-104279-choywmwd	323	17	apparatus	apparatus	NN
cord-104279-choywmwd	323	18	,	,	,
cord-104279-choywmwd	323	19	without	without	IN
cord-104279-choywmwd	323	20	prior	prior	JJ
cord-104279-choywmwd	323	21	delivery	delivery	NN
cord-104279-choywmwd	323	22	to	to	IN
cord-104279-choywmwd	323	23	the	the	DT
cord-104279-choywmwd	323	24	plasma	plasma	NN
cord-104279-choywmwd	323	25	membrane	membrane	NN
cord-104279-choywmwd	323	26	followed	follow	VBN
cord-104279-choywmwd	323	27	by	by	IN
cord-104279-choywmwd	323	28	endocytic	endocytic	JJ
cord-104279-choywmwd	323	29	uptake	uptake	NN
cord-104279-choywmwd	323	30	to	to	IN
cord-104279-choywmwd	323	31	the	the	DT
cord-104279-choywmwd	323	32	vacuole	vacuole	NN
cord-104279-choywmwd	323	33	.	.	.
cord-104279-choywmwd	324	1	Other	other	JJ
cord-104279-choywmwd	324	2	Golgi	Golgi	NNP
cord-104279-choywmwd	324	3	membrane	membrane	NN
cord-104279-choywmwd	324	4	proteins	protein	NNS
cord-104279-choywmwd	324	5	have	have	VBP
cord-104279-choywmwd	324	6	also	also	RB
cord-104279-choywmwd	324	7	been	be	VBN
cord-104279-choywmwd	324	8	shown	show	VBN
cord-104279-choywmwd	324	9	to	to	TO
cord-104279-choywmwd	324	10	be	be	VB
cord-104279-choywmwd	324	11	mislocalized	mislocalize	VBN
cord-104279-choywmwd	324	12	to	to	IN
cord-104279-choywmwd	324	13	the	the	DT
cord-104279-choywmwd	324	14	vacuole	vacuole	NN
cord-104279-choywmwd	324	15	rather	rather	RB
cord-104279-choywmwd	324	16	than	than	IN
cord-104279-choywmwd	324	17	the	the	DT
cord-104279-choywmwd	324	18	plasma	plasma	NN
cord-104279-choywmwd	324	19	membrane	membrane	NN
cord-104279-choywmwd	324	20	.	.	.
cord-104279-choywmwd	325	1	Both	both	DT
cord-104279-choywmwd	325	2	mutations	mutation	NNS
cord-104279-choywmwd	325	3	in	in	IN
cord-104279-choywmwd	325	4	the	the	DT
cord-104279-choywmwd	325	5	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	325	6	domain	domain	NN
cord-104279-choywmwd	325	7	of	of	IN
cord-104279-choywmwd	325	8	Kexlp	Kexlp	NNP
cord-104279-choywmwd	325	9	as	as	RB
cord-104279-choywmwd	325	10	well	well	RB
cord-104279-choywmwd	325	11	as	as	IN
cord-104279-choywmwd	325	12	its	-PRON-	PRP$
cord-104279-choywmwd	325	13	overproduction	overproduction	NN
cord-104279-choywmwd	325	14	result	result	NN
cord-104279-choywmwd	325	15	in	in	IN
cord-104279-choywmwd	325	16	mislocalization	mislocalization	NN
cord-104279-choywmwd	325	17	of	of	IN
cord-104279-choywmwd	325	18	the	the	DT
cord-104279-choywmwd	325	19	protein	protein	NN
cord-104279-choywmwd	325	20	to	to	IN
cord-104279-choywmwd	325	21	the	the	DT
cord-104279-choywmwd	325	22	vacuolar	vacuolar	NNP
cord-104279-choywmwd	325	23	membrane	membrane	NN
cord-104279-choywmwd	325	24	(	(	-LRB-
cord-104279-choywmwd	325	25	A.	A.	NNP
cord-104279-choywmwd	325	26	Cooper	Cooper	NNP
cord-104279-choywmwd	325	27	and	and	CC
cord-104279-choywmwd	325	28	H.	H.	NNP
cord-104279-choywmwd	325	29	Bussey	Bussey	NNP
cord-104279-choywmwd	325	30	,	,	,
cord-104279-choywmwd	325	31	manuscript	manuscript	NN
cord-104279-choywmwd	325	32	submitted	submit	VBN
cord-104279-choywmwd	325	33	for	for	IN
cord-104279-choywmwd	325	34	publication	publication	NN
cord-104279-choywmwd	325	35	)	)	-RRB-
cord-104279-choywmwd	325	36	.	.	.
cord-104279-choywmwd	326	1	Similarly	similarly	RB
cord-104279-choywmwd	326	2	,	,	,
cord-104279-choywmwd	326	3	a	a	DT
cord-104279-choywmwd	326	4	single	single	JJ
cord-104279-choywmwd	326	5	amino	amino	NN
cord-104279-choywmwd	326	6	acid	acid	NN
cord-104279-choywmwd	326	7	change	change	NN
cord-104279-choywmwd	326	8	in	in	IN
cord-104279-choywmwd	326	9	the	the	DT
cord-104279-choywmwd	326	10	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	326	11	domain	domain	NN
cord-104279-choywmwd	326	12	of	of	IN
cord-104279-choywmwd	326	13	Kex2p	Kex2p	NNP
cord-104279-choywmwd	326	14	results	result	NNS
cord-104279-choywmwd	326	15	in	in	IN
cord-104279-choywmwd	326	16	its	-PRON-	PRP$
cord-104279-choywmwd	326	17	transport	transport	NN
cord-104279-choywmwd	326	18	to	to	IN
cord-104279-choywmwd	326	19	the	the	DT
cord-104279-choywmwd	326	20	vacuole	vacuole	NN
cord-104279-choywmwd	326	21	(	(	-LRB-
cord-104279-choywmwd	326	22	C.	C.	NNP
cord-104279-choywmwd	326	23	Wilcox	Wilcox	NNP
cord-104279-choywmwd	326	24	,	,	,
cord-104279-choywmwd	326	25	K.	K.	NNP
cord-104279-choywmwd	326	26	Redding	Redding	NNP
cord-104279-choywmwd	326	27	,	,	,
cord-104279-choywmwd	326	28	R.	R.	NNP
cord-104279-choywmwd	326	29	Wright	Wright	NNP
cord-104279-choywmwd	326	30	,	,	,
cord-104279-choywmwd	326	31	and	and	CC
cord-104279-choywmwd	326	32	R.	R.	NNP
cord-104279-choywmwd	326	33	Fuller	Fuller	NNP
cord-104279-choywmwd	326	34	,	,	,
cord-104279-choywmwd	326	35	manuscript	manuscript	NN
cord-104279-choywmwd	326	36	submitted	submit	VBN
cord-104279-choywmwd	326	37	for	for	IN
cord-104279-choywmwd	326	38	publication	publication	NN
cord-104279-choywmwd	326	39	)	)	-RRB-
cord-104279-choywmwd	326	40	.	.	.
cord-104279-choywmwd	327	1	Whereas	whereas	IN
cord-104279-choywmwd	327	2	this	this	DT
cord-104279-choywmwd	327	3	result	result	NN
cord-104279-choywmwd	327	4	might	may	MD
cord-104279-choywmwd	327	5	appear	appear	VB
cord-104279-choywmwd	327	6	to	to	TO
cord-104279-choywmwd	327	7	conflict	conflict	VB
cord-104279-choywmwd	327	8	with	with	IN
cord-104279-choywmwd	327	9	earlier	early	JJR
cord-104279-choywmwd	327	10	published	publish	VBN
cord-104279-choywmwd	327	11	data	datum	NNS
cord-104279-choywmwd	327	12	on	on	IN
cord-104279-choywmwd	327	13	Kex2p	Kex2p	NNP
cord-104279-choywmwd	327	14	,	,	,
cord-104279-choywmwd	327	15	more	more	RBR
cord-104279-choywmwd	327	16	recent	recent	JJ
cord-104279-choywmwd	327	17	analysis	analysis	NN
cord-104279-choywmwd	327	18	indicates	indicate	VBZ
cord-104279-choywmwd	327	19	that	that	IN
cord-104279-choywmwd	327	20	membrane	membrane	NN
cord-104279-choywmwd	327	21	-	-	HYPH
cord-104279-choywmwd	327	22	bound	bind	VBN
cord-104279-choywmwd	327	23	forms	form	NNS
cord-104279-choywmwd	327	24	of	of	IN
cord-104279-choywmwd	327	25	Kex2p	Kex2p	NNP
cord-104279-choywmwd	327	26	that	that	WDT
cord-104279-choywmwd	327	27	fail	fail	VBP
cord-104279-choywmwd	327	28	to	to	TO
cord-104279-choywmwd	327	29	be	be	VB
cord-104279-choywmwd	327	30	retained	retain	VBN
cord-104279-choywmwd	327	31	in	in	IN
cord-104279-choywmwd	327	32	the	the	DT
cord-104279-choywmwd	327	33	Golgi	Golgi	NNP
cord-104279-choywmwd	327	34	are	be	VBP
cord-104279-choywmwd	327	35	found	find	VBN
cord-104279-choywmwd	327	36	in	in	IN
cord-104279-choywmwd	327	37	the	the	DT
cord-104279-choywmwd	327	38	vacuole	vacuole	NN
cord-104279-choywmwd	327	39	rather	rather	RB
cord-104279-choywmwd	327	40	than	than	IN
cord-104279-choywmwd	327	41	the	the	DT
cord-104279-choywmwd	327	42	plasma	plasma	NN
cord-104279-choywmwd	327	43	membrane	membrane	NN
cord-104279-choywmwd	327	44	,	,	,
cord-104279-choywmwd	327	45	when	when	WRB
cord-104279-choywmwd	327	46	these	these	DT
cord-104279-choywmwd	327	47	proteins	protein	NNS
cord-104279-choywmwd	327	48	are	be	VBP
cord-104279-choywmwd	327	49	expressed	express	VBN
cord-104279-choywmwd	327	50	at	at	IN
cord-104279-choywmwd	327	51	wild	wild	JJ
cord-104279-choywmwd	327	52	type	type	NN
cord-104279-choywmwd	327	53	or	or	CC
cord-104279-choywmwd	327	54	modestly	modestly	RB
cord-104279-choywmwd	327	55	elevated	elevated	JJ
cord-104279-choywmwd	327	56	levels	level	NNS
cord-104279-choywmwd	327	57	(	(	-LRB-
cord-104279-choywmwd	327	58	C.	C.	NNP
cord-104279-choywmwd	327	59	Wilcox	Wilcox	NNP
cord-104279-choywmwd	327	60	,	,	,
cord-104279-choywmwd	327	61	K.	K.	NNP
cord-104279-choywmwd	327	62	Redding	Redding	NNP
cord-104279-choywmwd	327	63	,	,	,
cord-104279-choywmwd	327	64	R.	R.	NNP
cord-104279-choywmwd	327	65	Wright	Wright	NNP
cord-104279-choywmwd	327	66	,	,	,
cord-104279-choywmwd	327	67	and	and	CC
cord-104279-choywmwd	327	68	R.	R.	NNP
cord-104279-choywmwd	327	69	Fuller	Fuller	NNP
cord-104279-choywmwd	327	70	,	,	,
cord-104279-choywmwd	327	71	manuscript	manuscript	NN
cord-104279-choywmwd	327	72	submitted	submit	VBN
cord-104279-choywmwd	327	73	for	for	IN
cord-104279-choywmwd	327	74	publication	publication	NN
cord-104279-choywmwd	327	75	)	)	-RRB-
cord-104279-choywmwd	327	76	.	.	.
cord-104279-choywmwd	328	1	Given	give	VBN
cord-104279-choywmwd	328	2	that	that	IN
cord-104279-choywmwd	328	3	several	several	JJ
cord-104279-choywmwd	328	4	Golgi	Golgi	NNP
cord-104279-choywmwd	328	5	membrane	membrane	NN
cord-104279-choywmwd	328	6	proteins	protein	NNS
cord-104279-choywmwd	328	7	all	all	DT
cord-104279-choywmwd	328	8	show	show	VBP
cord-104279-choywmwd	328	9	missorting	missorte	VBG
cord-104279-choywmwd	328	10	to	to	IN
cord-104279-choywmwd	328	11	the	the	DT
cord-104279-choywmwd	328	12	vacuole	vacuole	NN
cord-104279-choywmwd	328	13	when	when	WRB
cord-104279-choywmwd	328	14	either	either	CC
cord-104279-choywmwd	328	15	mutant	mutant	JJ
cord-104279-choywmwd	328	16	or	or	CC
cord-104279-choywmwd	328	17	overproduced	overproduced	JJ
cord-104279-choywmwd	328	18	,	,	,
cord-104279-choywmwd	328	19	the	the	DT
cord-104279-choywmwd	328	20	model	model	NN
cord-104279-choywmwd	328	21	we	-PRON-	PRP
cord-104279-choywmwd	328	22	favor	favor	VBP
cord-104279-choywmwd	328	23	to	to	TO
cord-104279-choywmwd	328	24	explain	explain	VB
cord-104279-choywmwd	328	25	these	these	DT
cord-104279-choywmwd	328	26	data	datum	NNS
cord-104279-choywmwd	328	27	is	be	VBZ
cord-104279-choywmwd	328	28	the	the	DT
cord-104279-choywmwd	328	29	vacuolar	vacuolar	NNP
cord-104279-choywmwd	328	30	default	default	NN
cord-104279-choywmwd	328	31	model	model	NN
cord-104279-choywmwd	328	32	,	,	,
cord-104279-choywmwd	328	33	which	which	WDT
cord-104279-choywmwd	328	34	states	state	VBZ
cord-104279-choywmwd	328	35	that	that	IN
cord-104279-choywmwd	328	36	vacuolar	vacuolar	NNP
cord-104279-choywmwd	328	37	membrane	membrane	NN
cord-104279-choywmwd	328	38	proteins	protein	NNS
cord-104279-choywmwd	328	39	do	do	VBP
cord-104279-choywmwd	328	40	not	not	RB
cord-104279-choywmwd	328	41	require	require	VB
cord-104279-choywmwd	328	42	sorting	sort	VBG
cord-104279-choywmwd	328	43	information	information	NN
cord-104279-choywmwd	328	44	,	,	,
cord-104279-choywmwd	328	45	because	because	IN
cord-104279-choywmwd	328	46	the	the	DT
cord-104279-choywmwd	328	47	default	default	NN
cord-104279-choywmwd	328	48	pathway	pathway	NN
cord-104279-choywmwd	328	49	for	for	IN
cord-104279-choywmwd	328	50	membrane	membrane	NN
cord-104279-choywmwd	328	51	proteins	protein	NNS
cord-104279-choywmwd	328	52	of	of	IN
cord-104279-choywmwd	328	53	the	the	DT
cord-104279-choywmwd	328	54	secretory	secretory	JJ
cord-104279-choywmwd	328	55	pathway	pathway	NN
cord-104279-choywmwd	328	56	leads	lead	VBZ
cord-104279-choywmwd	328	57	to	to	IN
cord-104279-choywmwd	328	58	the	the	DT
cord-104279-choywmwd	328	59	vacuole	vacuole	NN
cord-104279-choywmwd	328	60	.	.	.
cord-104279-choywmwd	329	1	Alternatively	alternatively	RB
cord-104279-choywmwd	329	2	,	,	,
cord-104279-choywmwd	329	3	one	one	PRP
cord-104279-choywmwd	329	4	could	could	MD
cord-104279-choywmwd	329	5	argue	argue	VB
cord-104279-choywmwd	329	6	that	that	IN
cord-104279-choywmwd	329	7	,	,	,
cord-104279-choywmwd	329	8	given	give	VBN
cord-104279-choywmwd	329	9	the	the	DT
cord-104279-choywmwd	329	10	similarity	similarity	NN
cord-104279-choywmwd	329	11	between	between	IN
cord-104279-choywmwd	329	12	DPAP	DPAP	NNP
cord-104279-choywmwd	329	13	B	B	NNP
cord-104279-choywmwd	329	14	and	and	CC
cord-104279-choywmwd	329	15	DPAP	DPAP	NNP
cord-104279-choywmwd	329	16	A	A	NNP
cord-104279-choywmwd	329	17	,	,	,
cord-104279-choywmwd	329	18	both	both	DT
cord-104279-choywmwd	329	19	proteins	protein	NNS
cord-104279-choywmwd	329	20	could	could	MD
cord-104279-choywmwd	329	21	contain	contain	VB
cord-104279-choywmwd	329	22	vacuolar	vacuolar	NNP
cord-104279-choywmwd	329	23	targeting	target	VBG
cord-104279-choywmwd	329	24	information	information	NN
cord-104279-choywmwd	329	25	in	in	IN
cord-104279-choywmwd	329	26	their	-PRON-	PRP$
cord-104279-choywmwd	329	27	transmembrane	transmembrane	NN
cord-104279-choywmwd	329	28	domains	domain	NNS
cord-104279-choywmwd	329	29	,	,	,
cord-104279-choywmwd	329	30	even	even	RB
cord-104279-choywmwd	329	31	though	though	IN
cord-104279-choywmwd	329	32	no	no	DT
cord-104279-choywmwd	329	33	significant	significant	JJ
cord-104279-choywmwd	329	34	similarity	similarity	NN
cord-104279-choywmwd	329	35	is	be	VBZ
cord-104279-choywmwd	329	36	apparent	apparent	JJ
cord-104279-choywmwd	329	37	from	from	IN
cord-104279-choywmwd	329	38	the	the	DT
cord-104279-choywmwd	329	39	amino	amino	NN
cord-104279-choywmwd	329	40	acid	acid	NN
cord-104279-choywmwd	329	41	sequence	sequence	NN
cord-104279-choywmwd	329	42	data	data	NN
cord-104279-choywmwd	329	43	(	(	-LRB-
cord-104279-choywmwd	329	44	C.	C.	NNP
cord-104279-choywmwd	329	45	A.	A.	NNP
cord-104279-choywmwd	329	46	Flanagan	Flanagan	NNP
cord-104279-choywmwd	329	47	,	,	,
cord-104279-choywmwd	329	48	13	13	CD
cord-104279-choywmwd	329	49	.	.	.
cord-104279-choywmwd	330	1	A.	A.	NNP
cord-104279-choywmwd	330	2	Barnes	Barnes	NNP
cord-104279-choywmwd	330	3	,	,	,
cord-104279-choywmwd	330	4	M.	M.	NNP
cord-104279-choywmwd	330	5	C.	C.	NNP
cord-104279-choywmwd	330	6	Flessel	Flessel	NNP
cord-104279-choywmwd	330	7	,	,	,
cord-104279-choywmwd	330	8	and	and	CC
cord-104279-choywmwd	330	9	J.	J.	NNP
cord-104279-choywmwd	330	10	Thorner	Thorner	NNP
cord-104279-choywmwd	330	11	,	,	,
cord-104279-choywmwd	330	12	manuscript	manuscript	NN
cord-104279-choywmwd	330	13	submitted	submit	VBN
cord-104279-choywmwd	330	14	for	for	IN
cord-104279-choywmwd	330	15	publication	publication	NN
cord-104279-choywmwd	330	16	)	)	-RRB-
cord-104279-choywmwd	330	17	.	.	.
cord-104279-choywmwd	331	1	Mutations	mutation	NNS
cord-104279-choywmwd	331	2	in	in	IN
cord-104279-choywmwd	331	3	the	the	DT
cord-104279-choywmwd	331	4	Golgi	Golgi	NNP
cord-104279-choywmwd	331	5	retention	retention	NN
cord-104279-choywmwd	331	6	signal	signal	NN
cord-104279-choywmwd	331	7	of	of	IN
cord-104279-choywmwd	331	8	DPAP	DPAP	NNP
cord-104279-choywmwd	331	9	A	A	NNP
cord-104279-choywmwd	331	10	,	,	,
cord-104279-choywmwd	331	11	or	or	CC
cord-104279-choywmwd	331	12	saturation	saturation	NN
cord-104279-choywmwd	331	13	of	of	IN
cord-104279-choywmwd	331	14	the	the	DT
cord-104279-choywmwd	331	15	retention	retention	NN
cord-104279-choywmwd	331	16	apparatus	apparatus	NN
cord-104279-choywmwd	331	17	,	,	,
cord-104279-choywmwd	331	18	would	would	MD
cord-104279-choywmwd	331	19	then	then	RB
cord-104279-choywmwd	331	20	result	result	VB
cord-104279-choywmwd	331	21	in	in	IN
cord-104279-choywmwd	331	22	the	the	DT
cord-104279-choywmwd	331	23	delivery	delivery	NN
cord-104279-choywmwd	331	24	of	of	IN
cord-104279-choywmwd	331	25	DPAP	DPAP	NNP
cord-104279-choywmwd	331	26	A	A	NNP
cord-104279-choywmwd	331	27	to	to	IN
cord-104279-choywmwd	331	28	the	the	DT
cord-104279-choywmwd	331	29	vacuole	vacuole	NN
cord-104279-choywmwd	331	30	.	.	.
cord-104279-choywmwd	332	1	This	this	DT
cord-104279-choywmwd	332	2	model	model	NN
cord-104279-choywmwd	332	3	requires	require	VBZ
cord-104279-choywmwd	332	4	that	that	IN
cord-104279-choywmwd	332	5	both	both	DT
cord-104279-choywmwd	332	6	Kex2p	Kex2p	NNP
cord-104279-choywmwd	332	7	and	and	CC
cord-104279-choywmwd	332	8	Kexlp	Kexlp	NNP
cord-104279-choywmwd	332	9	must	must	MD
cord-104279-choywmwd	332	10	also	also	RB
cord-104279-choywmwd	332	11	have	have	VB
cord-104279-choywmwd	332	12	cryptic	cryptic	JJ
cord-104279-choywmwd	332	13	vacuolar	vacuolar	JJ
cord-104279-choywmwd	332	14	targeting	target	VBG
cord-104279-choywmwd	332	15	signals	signal	NNS
cord-104279-choywmwd	332	16	.	.	.
cord-104279-choywmwd	333	1	With	with	IN
cord-104279-choywmwd	333	2	regard	regard	NN
cord-104279-choywmwd	333	3	to	to	IN
cord-104279-choywmwd	333	4	the	the	DT
cord-104279-choywmwd	333	5	cryptic	cryptic	JJ
cord-104279-choywmwd	333	6	vacuolar	vacuolar	JJ
cord-104279-choywmwd	333	7	targeting	target	VBG
cord-104279-choywmwd	333	8	signal	signal	NN
cord-104279-choywmwd	333	9	model	model	NN
cord-104279-choywmwd	333	10	,	,	,
cord-104279-choywmwd	333	11	we	-PRON-	PRP
cord-104279-choywmwd	333	12	have	have	VBP
cord-104279-choywmwd	333	13	found	find	VBN
cord-104279-choywmwd	333	14	that	that	DT
cord-104279-choywmwd	333	15	replacement	replacement	NN
cord-104279-choywmwd	333	16	of	of	IN
cord-104279-choywmwd	333	17	the	the	DT
cord-104279-choywmwd	333	18	membrane	membrane	NN
cord-104279-choywmwd	333	19	spanning	span	VBG
cord-104279-choywmwd	333	20	domains	domain	NNS
cord-104279-choywmwd	333	21	of	of	IN
cord-104279-choywmwd	333	22	DPAP	DPAP	NNP
cord-104279-choywmwd	333	23	A	A	NNP
cord-104279-choywmwd	333	24	and	and	CC
cord-104279-choywmwd	333	25	A22-AAA	a22-aaa	NN
cord-104279-choywmwd	333	26	with	with	IN
cord-104279-choywmwd	333	27	a	a	DT
cord-104279-choywmwd	333	28	21	21	CD
cord-104279-choywmwd	333	29	residue	residue	NN
cord-104279-choywmwd	333	30	hydrophobic	hydrophobic	JJ
cord-104279-choywmwd	333	31	sequence	sequence	NN
cord-104279-choywmwd	333	32	,	,	,
cord-104279-choywmwd	333	33	L(LALV)~	L(LALV)~	NNP
cord-104279-choywmwd	333	34	,	,	,
cord-104279-choywmwd	333	35	creating	create	VBG
cord-104279-choywmwd	333	36	the	the	DT
cord-104279-choywmwd	333	37	proteins	protein	NNS
cord-104279-choywmwd	333	38	AXA	AXA	NNP
cord-104279-choywmwd	333	39	and	and	CC
cord-104279-choywmwd	334	1	A22-AXA	a22-axa	NN
cord-104279-choywmwd	334	2	,	,	,
cord-104279-choywmwd	334	3	results	result	VBZ
cord-104279-choywmwd	334	4	in	in	IN
cord-104279-choywmwd	334	5	the	the	DT
cord-104279-choywmwd	334	6	retention	retention	NN
cord-104279-choywmwd	334	7	of	of	IN
cord-104279-choywmwd	334	8	AXA	AXA	NNP
cord-104279-choywmwd	334	9	in	in	IN
cord-104279-choywmwd	334	10	the	the	DT
cord-104279-choywmwd	334	11	Golgi	Golgi	NNP
cord-104279-choywmwd	334	12	and	and	CC
cord-104279-choywmwd	334	13	the	the	DT
cord-104279-choywmwd	334	14	transport	transport	NN
cord-104279-choywmwd	334	15	of	of	IN
cord-104279-choywmwd	334	16	A22-AXA	a22-axa	DT
cord-104279-choywmwd	334	17	to	to	IN
cord-104279-choywmwd	334	18	the	the	DT
cord-104279-choywmwd	334	19	vacuole	vacuole	NN
cord-104279-choywmwd	334	20	(	(	-LRB-
cord-104279-choywmwd	334	21	S.	S.	NNP
cord-104279-choywmwd	334	22	Nothwehr	Nothwehr	NNP
cord-104279-choywmwd	334	23	and	and	CC
cord-104279-choywmwd	334	24	T.	T.	NNP
cord-104279-choywmwd	334	25	Stevens	Stevens	NNP
cord-104279-choywmwd	334	26	,	,	,
cord-104279-choywmwd	334	27	unpublished	unpublished	JJ
cord-104279-choywmwd	334	28	data	datum	NNS
cord-104279-choywmwd	334	29	)	)	-RRB-
cord-104279-choywmwd	334	30	.	.	.
cord-104279-choywmwd	335	1	These	these	DT
cord-104279-choywmwd	335	2	data	datum	NNS
cord-104279-choywmwd	335	3	suggest	suggest	VBP
cord-104279-choywmwd	335	4	that	that	IN
cord-104279-choywmwd	335	5	the	the	DT
cord-104279-choywmwd	335	6	transmembrane	transmembrane	NN
cord-104279-choywmwd	335	7	domain	domain	NN
cord-104279-choywmwd	335	8	of	of	IN
cord-104279-choywmwd	335	9	DPAP	DPAP	NNP
cord-104279-choywmwd	335	10	A	A	NNP
cord-104279-choywmwd	335	11	does	do	VBZ
cord-104279-choywmwd	335	12	not	not	RB
cord-104279-choywmwd	335	13	contain	contain	VB
cord-104279-choywmwd	335	14	vacuolar	vacuolar	JJ
cord-104279-choywmwd	335	15	targeting	target	VBG
cord-104279-choywmwd	335	16	information	information	NN
cord-104279-choywmwd	335	17	,	,	,
cord-104279-choywmwd	335	18	and	and	CC
cord-104279-choywmwd	335	19	thus	thus	RB
cord-104279-choywmwd	335	20	support	support	VB
cord-104279-choywmwd	335	21	the	the	DT
cord-104279-choywmwd	335	22	vacuolar	vacuolar	NNP
cord-104279-choywmwd	335	23	default	default	NN
cord-104279-choywmwd	335	24	model	model	NN
cord-104279-choywmwd	335	25	.	.	.
cord-104279-choywmwd	336	1	The	the	DT
cord-104279-choywmwd	336	2	vacuolar	vacuolar	JJ
cord-104279-choywmwd	336	3	default	default	NN
cord-104279-choywmwd	336	4	model	model	NN
cord-104279-choywmwd	336	5	is	be	VBZ
cord-104279-choywmwd	336	6	directly	directly	RB
cord-104279-choywmwd	336	7	testable	testable	JJ
cord-104279-choywmwd	336	8	by	by	IN
cord-104279-choywmwd	336	9	analyzing	analyze	VBG
cord-104279-choywmwd	336	10	potential	potential	JJ
cord-104279-choywmwd	336	11	localization	localization	NN
cord-104279-choywmwd	336	12	signals	signal	NNS
cord-104279-choywmwd	336	13	of	of	IN
cord-104279-choywmwd	336	14	yeast	yeast	NN
cord-104279-choywmwd	336	15	plasma	plasma	NN
cord-104279-choywmwd	336	16	membrane	membrane	NN
cord-104279-choywmwd	336	17	proteins	protein	NNS
cord-104279-choywmwd	336	18	.	.	.
cord-104279-choywmwd	337	1	In	in	IN
cord-104279-choywmwd	337	2	apparent	apparent	JJ
cord-104279-choywmwd	337	3	conflict	conflict	NN
cord-104279-choywmwd	337	4	with	with	IN
cord-104279-choywmwd	337	5	the	the	DT
cord-104279-choywmwd	337	6	vacuolar	vacuolar	JJ
cord-104279-choywmwd	337	7	default	default	NN
cord-104279-choywmwd	337	8	model	model	NN
cord-104279-choywmwd	337	9	is	be	VBZ
cord-104279-choywmwd	337	10	the	the	DT
cord-104279-choywmwd	337	11	observation	observation	NN
cord-104279-choywmwd	337	12	that	that	IN
cord-104279-choywmwd	337	13	the	the	DT
cord-104279-choywmwd	337	14	majority	majority	NN
cord-104279-choywmwd	337	15	of	of	IN
cord-104279-choywmwd	337	16	Kex2p	Kex2p	NNP
cord-104279-choywmwd	337	17	(	(	-LRB-
cord-104279-choywmwd	337	18	70	70	CD
cord-104279-choywmwd	337	19	%	%	NN
cord-104279-choywmwd	337	20	)	)	-RRB-
cord-104279-choywmwd	337	21	is	be	VBZ
cord-104279-choywmwd	337	22	missorted	missorte	VBN
cord-104279-choywmwd	337	23	to	to	IN
cord-104279-choywmwd	337	24	the	the	DT
cord-104279-choywmwd	337	25	plasma	plasma	NN
cord-104279-choywmwd	337	26	membrane	membrane	NN
cord-104279-choywmwd	337	27	in	in	IN
cord-104279-choywmwd	337	28	cells	cell	NNS
cord-104279-choywmwd	337	29	deficient	deficient	JJ
cord-104279-choywmwd	337	30	for	for	IN
cord-104279-choywmwd	337	31	clathrin	clathrin	NN
cord-104279-choywmwd	337	32	heavy	heavy	JJ
cord-104279-choywmwd	337	33	chain	chain	NN
cord-104279-choywmwd	338	1	(	(	-LRB-
cord-104279-choywmwd	338	2	Payne	Payne	NNP
cord-104279-choywmwd	338	3	and	and	CC
cord-104279-choywmwd	338	4	Schekman	Schekman	NNP
cord-104279-choywmwd	338	5	,	,	,
cord-104279-choywmwd	338	6	1989	1989	CD
cord-104279-choywmwd	338	7	)	)	-RRB-
cord-104279-choywmwd	338	8	.	.	.
cord-104279-choywmwd	339	1	However	however	RB
cord-104279-choywmwd	339	2	,	,	,
cord-104279-choywmwd	339	3	DPAP	DPAP	NNP
cord-104279-choywmwd	339	4	A	A	NNP
cord-104279-choywmwd	339	5	is	be	VBZ
cord-104279-choywmwd	339	6	missorted	missorte	VBN
cord-104279-choywmwd	339	7	to	to	IN
cord-104279-choywmwd	339	8	the	the	DT
cord-104279-choywmwd	339	9	cell	cell	NN
cord-104279-choywmwd	339	10	surface	surface	NN
cord-104279-choywmwd	339	11	to	to	IN
cord-104279-choywmwd	339	12	a	a	DT
cord-104279-choywmwd	339	13	lesser	less	JJR
cord-104279-choywmwd	339	14	extent	extent	NN
cord-104279-choywmwd	339	15	(	(	-LRB-
cord-104279-choywmwd	339	16	30	30	CD
cord-104279-choywmwd	339	17	%	%	NN
cord-104279-choywmwd	339	18	;	;	:
cord-104279-choywmwd	339	19	Seeger	Seeger	NNP
cord-104279-choywmwd	339	20	and	and	CC
cord-104279-choywmwd	339	21	Payne	Payne	NNP
cord-104279-choywmwd	339	22	,	,	,
cord-104279-choywmwd	339	23	1992a	1992a	CD
cord-104279-choywmwd	339	24	)	)	-RRB-
cord-104279-choywmwd	339	25	.	.	.
cord-104279-choywmwd	340	1	It	-PRON-	PRP
cord-104279-choywmwd	340	2	is	be	VBZ
cord-104279-choywmwd	340	3	possible	possible	JJ
cord-104279-choywmwd	340	4	that	that	IN
cord-104279-choywmwd	340	5	the	the	DT
cord-104279-choywmwd	340	6	majority	majority	NN
cord-104279-choywmwd	340	7	of	of	IN
cord-104279-choywmwd	340	8	DPAP	DPAP	NNP
cord-104279-choywmwd	340	9	A	A	NNP
cord-104279-choywmwd	340	10	and	and	CC
cord-104279-choywmwd	340	11	a	a	DT
cord-104279-choywmwd	340	12	significant	significant	JJ
cord-104279-choywmwd	340	13	percentage	percentage	NN
cord-104279-choywmwd	340	14	of	of	IN
cord-104279-choywmwd	340	15	Kex2p	Kex2p	NNP
cord-104279-choywmwd	340	16	are	be	VBP
cord-104279-choywmwd	340	17	missorted	missorte	VBN
cord-104279-choywmwd	340	18	to	to	IN
cord-104279-choywmwd	340	19	the	the	DT
cord-104279-choywmwd	340	20	vacuole	vacuole	NN
cord-104279-choywmwd	340	21	in	in	IN
cord-104279-choywmwd	340	22	cells	cell	NNS
cord-104279-choywmwd	340	23	lacking	lack	VBG
cord-104279-choywmwd	340	24	clathrin	clathrin	NN
cord-104279-choywmwd	340	25	heavy	heavy	JJ
cord-104279-choywmwd	340	26	chain	chain	NN
cord-104279-choywmwd	340	27	.	.	.
cord-104279-choywmwd	341	1	Mutations	mutation	NNS
cord-104279-choywmwd	341	2	in	in	IN
cord-104279-choywmwd	341	3	clathrin	clathrin	NN
cord-104279-choywmwd	341	4	may	may	MD
cord-104279-choywmwd	341	5	affect	affect	VB
cord-104279-choywmwd	341	6	both	both	CC
cord-104279-choywmwd	341	7	the	the	DT
cord-104279-choywmwd	341	8	retention	retention	NN
cord-104279-choywmwd	341	9	of	of	IN
cord-104279-choywmwd	341	10	Golgi	Golgi	NNP
cord-104279-choywmwd	341	11	membrane	membrane	NN
cord-104279-choywmwd	341	12	proteins	protein	NNS
cord-104279-choywmwd	341	13	and	and	CC
cord-104279-choywmwd	341	14	the	the	DT
cord-104279-choywmwd	341	15	functional	functional	JJ
cord-104279-choywmwd	341	16	integrity	integrity	NN
cord-104279-choywmwd	341	17	of	of	IN
cord-104279-choywmwd	341	18	the	the	DT
cord-104279-choywmwd	341	19	sorting	sorting	NN
cord-104279-choywmwd	341	20	pathway	pathway	NN
cord-104279-choywmwd	341	21	(	(	-LRB-
cord-104279-choywmwd	341	22	Seeger	Seeger	NNP
cord-104279-choywmwd	341	23	and	and	CC
cord-104279-choywmwd	341	24	Payne	Payne	NNP
cord-104279-choywmwd	341	25	,	,	,
cord-104279-choywmwd	341	26	1992b	1992b	CD
cord-104279-choywmwd	341	27	)	)	-RRB-
cord-104279-choywmwd	341	28	.	.	.
cord-104279-choywmwd	342	1	This	this	DT
cord-104279-choywmwd	342	2	could	could	MD
cord-104279-choywmwd	342	3	obscure	obscure	VB
cord-104279-choywmwd	342	4	the	the	DT
cord-104279-choywmwd	342	5	default	default	NN
cord-104279-choywmwd	342	6	pathway	pathway	NN
cord-104279-choywmwd	342	7	for	for	IN
cord-104279-choywmwd	342	8	membrane	membrane	NN
cord-104279-choywmwd	342	9	proteins	protein	NNS
cord-104279-choywmwd	342	10	,	,	,
cord-104279-choywmwd	342	11	resulting	result	VBG
cord-104279-choywmwd	342	12	in	in	IN
cord-104279-choywmwd	342	13	the	the	DT
cord-104279-choywmwd	342	14	transport	transport	NN
cord-104279-choywmwd	342	15	of	of	IN
cord-104279-choywmwd	342	16	Golgi	Golgi	NNP
cord-104279-choywmwd	342	17	membrane	membrane	NN
cord-104279-choywmwd	342	18	proteins	protein	NNS
cord-104279-choywmwd	342	19	to	to	IN
cord-104279-choywmwd	342	20	the	the	DT
cord-104279-choywmwd	342	21	plasma	plasma	NN
cord-104279-choywmwd	342	22	membrane	membrane	NN
cord-104279-choywmwd	342	23	as	as	RB
cord-104279-choywmwd	342	24	well	well	RB
cord-104279-choywmwd	342	25	as	as	IN
cord-104279-choywmwd	342	26	the	the	DT
cord-104279-choywmwd	342	27	vacuole	vacuole	NN
cord-104279-choywmwd	342	28	.	.	.
cord-104279-choywmwd	343	1	An	an	DT
cord-104279-choywmwd	343	2	alternative	alternative	JJ
cord-104279-choywmwd	343	3	explanation	explanation	NN
cord-104279-choywmwd	343	4	of	of	IN
cord-104279-choywmwd	343	5	the	the	DT
cord-104279-choywmwd	343	6	data	data	NN
cord-104279-choywmwd	343	7	is	be	VBZ
cord-104279-choywmwd	343	8	that	that	IN
cord-104279-choywmwd	343	9	one	one	CD
cord-104279-choywmwd	343	10	or	or	CC
cord-104279-choywmwd	343	11	more	more	JJR
cord-104279-choywmwd	343	12	of	of	IN
cord-104279-choywmwd	343	13	the	the	DT
cord-104279-choywmwd	343	14	DPAP	DPAP	NNP
cord-104279-choywmwd	343	15	A	A	NNP
cord-104279-choywmwd	343	16	and	and	CC
cord-104279-choywmwd	343	17	DPAP	DPAP	NNP
cord-104279-choywmwd	343	18	B	B	NNP
cord-104279-choywmwd	343	19	fusion	fusion	NN
cord-104279-choywmwd	343	20	or	or	CC
cord-104279-choywmwd	343	21	mutant	mutant	JJ
cord-104279-choywmwd	343	22	proteins	protein	NNS
cord-104279-choywmwd	343	23	analyzed	analyze	VBN
cord-104279-choywmwd	343	24	in	in	IN
cord-104279-choywmwd	343	25	this	this	DT
cord-104279-choywmwd	343	26	study	study	NN
cord-104279-choywmwd	343	27	were	be	VBD
cord-104279-choywmwd	343	28	in	in	IN
cord-104279-choywmwd	343	29	a	a	DT
cord-104279-choywmwd	343	30	partially	partially	RB
cord-104279-choywmwd	343	31	unfolded	unfold	VBN
cord-104279-choywmwd	343	32	state	state	NN
cord-104279-choywmwd	343	33	,	,	,
cord-104279-choywmwd	343	34	and	and	CC
cord-104279-choywmwd	343	35	thus	thus	RB
cord-104279-choywmwd	343	36	recognized	recognize	VBN
cord-104279-choywmwd	343	37	as	as	IN
cord-104279-choywmwd	343	38	abnormal	abnormal	JJ
cord-104279-choywmwd	343	39	and	and	CC
cord-104279-choywmwd	343	40	transported	transport	VBN
cord-104279-choywmwd	343	41	directly	directly	RB
cord-104279-choywmwd	343	42	from	from	IN
cord-104279-choywmwd	343	43	the	the	DT
cord-104279-choywmwd	343	44	ER	ER	NNP
cord-104279-choywmwd	343	45	to	to	IN
cord-104279-choywmwd	343	46	the	the	DT
cord-104279-choywmwd	343	47	vacuole	vacuole	NN
cord-104279-choywmwd	343	48	via	via	IN
cord-104279-choywmwd	343	49	a	a	DT
cord-104279-choywmwd	343	50	"	"	``
cord-104279-choywmwd	343	51	garbage	garbage	NN
cord-104279-choywmwd	343	52	"	"	''
cord-104279-choywmwd	343	53	pathway	pathway	NN
cord-104279-choywmwd	343	54	.	.	.
cord-104279-choywmwd	344	1	There	there	EX
cord-104279-choywmwd	344	2	is	be	VBZ
cord-104279-choywmwd	344	3	no	no	DT
cord-104279-choywmwd	344	4	clear	clear	JJ
cord-104279-choywmwd	344	5	evidence	evidence	NN
cord-104279-choywmwd	344	6	for	for	IN
cord-104279-choywmwd	344	7	the	the	DT
cord-104279-choywmwd	344	8	existence	existence	NN
cord-104279-choywmwd	344	9	of	of	IN
cord-104279-choywmwd	344	10	such	such	PDT
cord-104279-choywmwd	344	11	a	a	DT
cord-104279-choywmwd	344	12	pathway	pathway	NN
cord-104279-choywmwd	344	13	in	in	IN
cord-104279-choywmwd	344	14	yeast	yeast	NN
cord-104279-choywmwd	344	15	or	or	CC
cord-104279-choywmwd	344	16	animal	animal	NN
cord-104279-choywmwd	344	17	cells	cell	NNS
cord-104279-choywmwd	344	18	.	.	.
cord-104279-choywmwd	345	1	However	however	RB
cord-104279-choywmwd	345	2	,	,	,
cord-104279-choywmwd	345	3	several	several	JJ
cord-104279-choywmwd	345	4	cases	case	NNS
cord-104279-choywmwd	345	5	have	have	VBP
cord-104279-choywmwd	345	6	been	be	VBN
cord-104279-choywmwd	345	7	described	describe	VBN
cord-104279-choywmwd	345	8	in	in	IN
cord-104279-choywmwd	345	9	animal	animal	NN
cord-104279-choywmwd	345	10	cells	cell	NNS
cord-104279-choywmwd	345	11	in	in	IN
cord-104279-choywmwd	345	12	which	which	WDT
cord-104279-choywmwd	345	13	non	non	JJ
cord-104279-choywmwd	345	14	-	-	JJ
cord-104279-choywmwd	345	15	lysosomal	lysosomal	JJ
cord-104279-choywmwd	345	16	membrane	membrane	NN
cord-104279-choywmwd	345	17	proteins	protein	NNS
cord-104279-choywmwd	345	18	were	be	VBD
cord-104279-choywmwd	345	19	transported	transport	VBN
cord-104279-choywmwd	345	20	to	to	IN
cord-104279-choywmwd	345	21	lysosomes	lysosome	NNS
cord-104279-choywmwd	345	22	,	,	,
cord-104279-choywmwd	345	23	either	either	CC
cord-104279-choywmwd	345	24	due	due	IN
cord-104279-choywmwd	345	25	to	to	IN
cord-104279-choywmwd	345	26	mutations	mutation	NNS
cord-104279-choywmwd	345	27	(	(	-LRB-
cord-104279-choywmwd	345	28	Armstrong	Armstrong	NNP
cord-104279-choywmwd	345	29	et	et	NNP
cord-104279-choywmwd	345	30	al	al	NNP
cord-104279-choywmwd	345	31	.	.	NNP
cord-104279-choywmwd	345	32	,	,	,
cord-104279-choywmwd	345	33	1990	1990	CD
cord-104279-choywmwd	345	34	)	)	-RRB-
cord-104279-choywmwd	345	35	,	,	,
cord-104279-choywmwd	345	36	or	or	CC
cord-104279-choywmwd	345	37	in	in	IN
cord-104279-choywmwd	345	38	the	the	DT
cord-104279-choywmwd	345	39	case	case	NN
cord-104279-choywmwd	345	40	of	of	IN
cord-104279-choywmwd	345	41	the	the	DT
cord-104279-choywmwd	345	42	heptameric	heptameric	NNP
cord-104279-choywmwd	345	43	T	T	NNP
cord-104279-choywmwd	345	44	-	-	HYPH
cord-104279-choywmwd	345	45	cell	cell	NN
cord-104279-choywmwd	345	46	antigen	antigen	NN
cord-104279-choywmwd	345	47	receptor	receptor	NN
cord-104279-choywmwd	345	48	,	,	,
cord-104279-choywmwd	345	49	complexes	complex	NNS
cord-104279-choywmwd	345	50	lacking	lack	VBG
cord-104279-choywmwd	345	51	the	the	DT
cord-104279-choywmwd	345	52	~	~	:
cord-104279-choywmwd	345	53	"	"	``
cord-104279-choywmwd	345	54	subunit	subunit	NN
cord-104279-choywmwd	345	55	are	be	VBP
cord-104279-choywmwd	345	56	degraded	degrade	VBN
cord-104279-choywmwd	345	57	in	in	IN
cord-104279-choywmwd	345	58	lysosomes	lysosome	NNS
cord-104279-choywmwd	345	59	(	(	-LRB-
cord-104279-choywmwd	345	60	Minami	Minami	NNP
cord-104279-choywmwd	345	61	et	et	FW
cord-104279-choywmwd	345	62	al	al	NNP
cord-104279-choywmwd	345	63	.	.	NNP
cord-104279-choywmwd	345	64	,	,	,
cord-104279-choywmwd	345	65	1987	1987	CD
cord-104279-choywmwd	345	66	)	)	-RRB-
cord-104279-choywmwd	345	67	.	.	.
cord-104279-choywmwd	346	1	In	in	IN
cord-104279-choywmwd	346	2	these	these	DT
cord-104279-choywmwd	346	3	cases	case	NNS
cord-104279-choywmwd	346	4	,	,	,
cord-104279-choywmwd	346	5	the	the	DT
cord-104279-choywmwd	346	6	proteins	protein	NNS
cord-104279-choywmwd	346	7	were	be	VBD
cord-104279-choywmwd	346	8	transported	transport	VBN
cord-104279-choywmwd	346	9	through	through	IN
cord-104279-choywmwd	346	10	the	the	DT
cord-104279-choywmwd	346	11	Golgi	Golgi	NNP
cord-104279-choywmwd	346	12	apparatus	apparatus	NN
cord-104279-choywmwd	346	13	,	,	,
cord-104279-choywmwd	346	14	and	and	CC
cord-104279-choywmwd	346	15	it	-PRON-	PRP
cord-104279-choywmwd	346	16	is	be	VBZ
cord-104279-choywmwd	346	17	unclear	unclear	JJ
cord-104279-choywmwd	346	18	whether	whether	IN
cord-104279-choywmwd	346	19	the	the	DT
cord-104279-choywmwd	346	20	proteins	protein	NNS
cord-104279-choywmwd	346	21	were	be	VBD
cord-104279-choywmwd	346	22	transported	transport	VBN
cord-104279-choywmwd	346	23	to	to	IN
cord-104279-choywmwd	346	24	lysosomes	lysosome	NNS
cord-104279-choywmwd	346	25	by	by	IN
cord-104279-choywmwd	346	26	a	a	DT
cord-104279-choywmwd	346	27	"	"	``
cord-104279-choywmwd	346	28	garbage	garbage	NN
cord-104279-choywmwd	346	29	"	"	''
cord-104279-choywmwd	346	30	pathway	pathway	NN
cord-104279-choywmwd	346	31	,	,	,
cord-104279-choywmwd	346	32	or	or	CC
cord-104279-choywmwd	346	33	by	by	IN
cord-104279-choywmwd	346	34	the	the	DT
cord-104279-choywmwd	346	35	uncovering	uncovering	NN
cord-104279-choywmwd	346	36	of	of	IN
cord-104279-choywmwd	346	37	a	a	DT
cord-104279-choywmwd	346	38	cryptic	cryptic	JJ
cord-104279-choywmwd	346	39	lysosomal	lysosomal	NN
cord-104279-choywmwd	346	40	targeting	targeting	NN
cord-104279-choywmwd	346	41	signal	signal	NN
cord-104279-choywmwd	346	42	.	.	.
cord-104279-choywmwd	347	1	In	in	IN
cord-104279-choywmwd	347	2	the	the	DT
cord-104279-choywmwd	347	3	great	great	JJ
cord-104279-choywmwd	347	4	majority	majority	NN
cord-104279-choywmwd	347	5	of	of	IN
cord-104279-choywmwd	347	6	cases	case	NNS
cord-104279-choywmwd	347	7	,	,	,
cord-104279-choywmwd	347	8	mislocalized	mislocalize	VBD
cord-104279-choywmwd	347	9	membrane	membrane	NN
cord-104279-choywmwd	347	10	proteins	protein	NNS
cord-104279-choywmwd	347	11	of	of	IN
cord-104279-choywmwd	347	12	the	the	DT
cord-104279-choywmwd	347	13	secretory	secretory	JJ
cord-104279-choywmwd	347	14	pathway	pathway	NN
cord-104279-choywmwd	347	15	in	in	IN
cord-104279-choywmwd	347	16	animal	animal	NN
cord-104279-choywmwd	347	17	cells	cell	NNS
cord-104279-choywmwd	347	18	accumulate	accumulate	VBP
cord-104279-choywmwd	347	19	at	at	IN
cord-104279-choywmwd	347	20	the	the	DT
cord-104279-choywmwd	347	21	plasma	plasma	NN
cord-104279-choywmwd	347	22	membrane	membrane	NN
cord-104279-choywmwd	347	23	,	,	,
cord-104279-choywmwd	347	24	which	which	WDT
cord-104279-choywmwd	347	25	is	be	VBZ
cord-104279-choywmwd	347	26	presumed	presume	VBN
cord-104279-choywmwd	347	27	to	to	TO
cord-104279-choywmwd	347	28	be	be	VB
cord-104279-choywmwd	347	29	the	the	DT
cord-104279-choywmwd	347	30	default	default	NN
cord-104279-choywmwd	347	31	destination	destination	NN
cord-104279-choywmwd	347	32	for	for	IN
cord-104279-choywmwd	347	33	these	these	DT
cord-104279-choywmwd	347	34	proteins	protein	NNS
cord-104279-choywmwd	347	35	(	(	-LRB-
cord-104279-choywmwd	347	36	e.g.	e.g.	RB
cord-104279-choywmwd	347	37	,	,	,
cord-104279-choywmwd	347	38	Williams	Williams	NNP
cord-104279-choywmwd	347	39	and	and	CC
cord-104279-choywmwd	347	40	Fukuda	Fukuda	NNP
cord-104279-choywmwd	347	41	,	,	,
cord-104279-choywmwd	347	42	1990	1990	CD
cord-104279-choywmwd	347	43	)	)	-RRB-
cord-104279-choywmwd	347	44	.	.	.
cord-104279-choywmwd	348	1	It	-PRON-	PRP
cord-104279-choywmwd	348	2	is	be	VBZ
cord-104279-choywmwd	348	3	well	well	RB
cord-104279-choywmwd	348	4	established	establish	VBN
cord-104279-choywmwd	348	5	that	that	IN
cord-104279-choywmwd	348	6	proteins	protein	NNS
cord-104279-choywmwd	348	7	that	that	WDT
cord-104279-choywmwd	348	8	are	be	VBP
cord-104279-choywmwd	348	9	slow	slow	JJ
cord-104279-choywmwd	348	10	to	to	TO
cord-104279-choywmwd	348	11	reach	reach	VB
cord-104279-choywmwd	348	12	the	the	DT
cord-104279-choywmwd	348	13	folded	fold	VBN
cord-104279-choywmwd	348	14	state	state	NN
cord-104279-choywmwd	348	15	are	be	VBP
cord-104279-choywmwd	348	16	retained	retain	VBN
cord-104279-choywmwd	348	17	in	in	IN
cord-104279-choywmwd	348	18	the	the	DT
cord-104279-choywmwd	348	19	ER	ER	NNP
cord-104279-choywmwd	348	20	due	due	IN
cord-104279-choywmwd	348	21	to	to	IN
cord-104279-choywmwd	348	22	the	the	DT
cord-104279-choywmwd	348	23	action	action	NN
cord-104279-choywmwd	348	24	of	of	IN
cord-104279-choywmwd	348	25	proteins	protein	NNS
cord-104279-choywmwd	348	26	such	such	JJ
cord-104279-choywmwd	348	27	as	as	IN
cord-104279-choywmwd	348	28	BiP	BiP	NNP
cord-104279-choywmwd	348	29	(	(	-LRB-
cord-104279-choywmwd	348	30	Pelham	Pelham	NNP
cord-104279-choywmwd	348	31	,	,	,
cord-104279-choywmwd	348	32	1989	1989	CD
cord-104279-choywmwd	348	33	;	;	:
cord-104279-choywmwd	348	34	Rothman	Rothman	NNP
cord-104279-choywmwd	348	35	,	,	,
cord-104279-choywmwd	348	36	1989	1989	CD
cord-104279-choywmwd	348	37	)	)	-RRB-
cord-104279-choywmwd	348	38	,	,	,
cord-104279-choywmwd	348	39	and	and	CC
cord-104279-choywmwd	348	40	proteins	protein	NNS
cord-104279-choywmwd	348	41	that	that	WDT
cord-104279-choywmwd	348	42	are	be	VBP
cord-104279-choywmwd	348	43	unable	unable	JJ
cord-104279-choywmwd	348	44	to	to	TO
cord-104279-choywmwd	348	45	fold	fold	VB
cord-104279-choywmwd	348	46	or	or	CC
cord-104279-choywmwd	348	47	oligomerize	oligomerize	NNP
cord-104279-choywmwd	348	48	properly	properly	RB
cord-104279-choywmwd	348	49	are	be	VBP
cord-104279-choywmwd	348	50	degraded	degrade	VBN
cord-104279-choywmwd	348	51	in	in	IN
cord-104279-choywmwd	348	52	the	the	DT
cord-104279-choywmwd	348	53	ER	ER	NNP
cord-104279-choywmwd	348	54	(	(	-LRB-
cord-104279-choywmwd	348	55	Klausner	Klausner	NNP
cord-104279-choywmwd	348	56	and	and	CC
cord-104279-choywmwd	348	57	Sitia	Sitia	NNP
cord-104279-choywmwd	348	58	,	,	,
cord-104279-choywmwd	348	59	1990	1990	CD
cord-104279-choywmwd	348	60	)	)	-RRB-
cord-104279-choywmwd	348	61	.	.	.
cord-104279-choywmwd	349	1	Several	several	JJ
cord-104279-choywmwd	349	2	of	of	IN
cord-104279-choywmwd	349	3	the	the	DT
cord-104279-choywmwd	349	4	proteins	protein	NNS
cord-104279-choywmwd	349	5	analyzed	analyze	VBN
cord-104279-choywmwd	349	6	in	in	IN
cord-104279-choywmwd	349	7	this	this	DT
cord-104279-choywmwd	349	8	study	study	NN
cord-104279-choywmwd	349	9	(	(	-LRB-
cord-104279-choywmwd	349	10	i.e.	i.e.	FW
cord-104279-choywmwd	349	11	,	,	,
cord-104279-choywmwd	349	12	A20-BB	A20-BB	NNP
cord-104279-choywmwd	349	13	,	,	,
cord-104279-choywmwd	349	14	A27-BB	A27-BB	NNP
cord-104279-choywmwd	349	15	,	,	,
cord-104279-choywmwd	349	16	A22-AAA	a22-aaa	CC
cord-104279-choywmwd	349	17	,	,	,
cord-104279-choywmwd	349	18	and	and	CC
cord-104279-choywmwd	349	19	A22-AA	a22-aa	NN
cord-104279-choywmwd	349	20	-	-	HYPH
cord-104279-choywmwd	349	21	B	B	NNP
cord-104279-choywmwd	349	22	)	)	-RRB-
cord-104279-choywmwd	349	23	showed	show	VBD
cord-104279-choywmwd	349	24	increased	increase	VBN
cord-104279-choywmwd	349	25	retention	retention	NN
cord-104279-choywmwd	349	26	in	in	IN
cord-104279-choywmwd	349	27	the	the	DT
cord-104279-choywmwd	349	28	ER	ER	NNP
cord-104279-choywmwd	349	29	.	.	.
cord-104279-choywmwd	350	1	However	however	RB
cord-104279-choywmwd	350	2	,	,	,
cord-104279-choywmwd	350	3	all	all	DT
cord-104279-choywmwd	350	4	of	of	IN
cord-104279-choywmwd	350	5	these	these	DT
cord-104279-choywmwd	350	6	proteins	protein	NNS
cord-104279-choywmwd	350	7	eventually	eventually	RB
cord-104279-choywmwd	350	8	exited	exit	VBD
cord-104279-choywmwd	350	9	the	the	DT
cord-104279-choywmwd	350	10	ER	ER	NNP
cord-104279-choywmwd	350	11	,	,	,
cord-104279-choywmwd	350	12	were	be	VBD
cord-104279-choywmwd	350	13	enzymatically	enzymatically	RB
cord-104279-choywmwd	350	14	active	active	JJ
cord-104279-choywmwd	350	15	,	,	,
cord-104279-choywmwd	350	16	and	and	CC
cord-104279-choywmwd	350	17	were	be	VBD
cord-104279-choywmwd	350	18	transported	transport	VBN
cord-104279-choywmwd	350	19	through	through	IN
cord-104279-choywmwd	350	20	the	the	DT
cord-104279-choywmwd	350	21	Golgi	Golgi	NNP
cord-104279-choywmwd	350	22	complex	complex	NN
cord-104279-choywmwd	350	23	to	to	IN
cord-104279-choywmwd	350	24	the	the	DT
cord-104279-choywmwd	350	25	vacuole	vacuole	NN
cord-104279-choywmwd	350	26	.	.	.
cord-104279-choywmwd	351	1	The	the	DT
cord-104279-choywmwd	351	2	22-amino	22-amino	CD
cord-104279-choywmwd	351	3	acid	acid	NN
cord-104279-choywmwd	351	4	segment	segment	NN
cord-104279-choywmwd	351	5	of	of	IN
cord-104279-choywmwd	351	6	the	the	DT
cord-104279-choywmwd	351	7	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	351	8	domain	domain	NN
cord-104279-choywmwd	351	9	of	of	IN
cord-104279-choywmwd	351	10	DPAP	DPAP	NNP
cord-104279-choywmwd	351	11	A	A	NNP
cord-104279-choywmwd	351	12	presumably	presumably	RB
cord-104279-choywmwd	351	13	contains	contain	VBZ
cord-104279-choywmwd	351	14	the	the	DT
cord-104279-choywmwd	351	15	recognition	recognition	NN
cord-104279-choywmwd	351	16	domain	domain	NN
cord-104279-choywmwd	351	17	for	for	IN
cord-104279-choywmwd	351	18	a	a	DT
cord-104279-choywmwd	351	19	"	"	``
cord-104279-choywmwd	351	20	retention	retention	NN
cord-104279-choywmwd	351	21	protein	protein	NN
cord-104279-choywmwd	351	22	"	"	''
cord-104279-choywmwd	351	23	of	of	IN
cord-104279-choywmwd	351	24	the	the	DT
cord-104279-choywmwd	351	25	Golgi	Golgi	NNP
cord-104279-choywmwd	351	26	apparatus	apparatus	NN
cord-104279-choywmwd	351	27	.	.	.
cord-104279-choywmwd	352	1	This	this	DT
cord-104279-choywmwd	352	2	protein	protein	NN
cord-104279-choywmwd	352	3	could	could	MD
cord-104279-choywmwd	352	4	be	be	VB
cord-104279-choywmwd	352	5	a	a	DT
cord-104279-choywmwd	352	6	permanent	permanent	JJ
cord-104279-choywmwd	352	7	resident	resident	NN
cord-104279-choywmwd	352	8	of	of	IN
cord-104279-choywmwd	352	9	the	the	DT
cord-104279-choywmwd	352	10	Golgi	Golgi	NNP
cord-104279-choywmwd	352	11	apparatus	apparatus	NN
cord-104279-choywmwd	352	12	;	;	:
cord-104279-choywmwd	352	13	alternatively	alternatively	RB
cord-104279-choywmwd	352	14	,	,	,
cord-104279-choywmwd	352	15	the	the	DT
cord-104279-choywmwd	352	16	"	"	``
cord-104279-choywmwd	352	17	retention	retention	NN
cord-104279-choywmwd	352	18	protein	protein	NN
cord-104279-choywmwd	352	19	"	"	''
cord-104279-choywmwd	352	20	could	could	MD
cord-104279-choywmwd	352	21	reside	reside	VB
cord-104279-choywmwd	352	22	in	in	IN
cord-104279-choywmwd	352	23	a	a	DT
cord-104279-choywmwd	352	24	post	post	JJ
cord-104279-choywmwd	352	25	-	-	JJ
cord-104279-choywmwd	352	26	Golgi	golgi	JJ
cord-104279-choywmwd	352	27	compartment	compartment	NN
cord-104279-choywmwd	352	28	and	and	CC
cord-104279-choywmwd	352	29	function	function	NN
cord-104279-choywmwd	352	30	as	as	IN
cord-104279-choywmwd	352	31	a	a	DT
cord-104279-choywmwd	352	32	salvage	salvage	NN
cord-104279-choywmwd	352	33	receptor	receptor	NN
cord-104279-choywmwd	352	34	,	,	,
cord-104279-choywmwd	352	35	such	such	JJ
cord-104279-choywmwd	352	36	as	as	IN
cord-104279-choywmwd	352	37	the	the	DT
cord-104279-choywmwd	352	38	proposed	propose	VBN
cord-104279-choywmwd	352	39	receptor	receptor	NN
cord-104279-choywmwd	352	40	for	for	IN
cord-104279-choywmwd	352	41	soluble	soluble	JJ
cord-104279-choywmwd	352	42	ER	ER	NNP
cord-104279-choywmwd	352	43	proteins	protein	NNS
cord-104279-choywmwd	352	44	(	(	-LRB-
cord-104279-choywmwd	352	45	Pelham	Pelham	NNP
cord-104279-choywmwd	352	46	,	,	,
cord-104279-choywmwd	352	47	1989	1989	CD
cord-104279-choywmwd	352	48	)	)	-RRB-
cord-104279-choywmwd	352	49	.	.	.
cord-104279-choywmwd	353	1	It	-PRON-	PRP
cord-104279-choywmwd	353	2	is	be	VBZ
cord-104279-choywmwd	353	3	interesting	interesting	JJ
cord-104279-choywmwd	353	4	to	to	TO
cord-104279-choywmwd	353	5	note	note	VB
cord-104279-choywmwd	353	6	that	that	IN
cord-104279-choywmwd	353	7	the	the	DT
cord-104279-choywmwd	353	8	22-amino	22-amino	CD
cord-104279-choywmwd	353	9	acid	acid	NN
cord-104279-choywmwd	353	10	stretch	stretch	NN
cord-104279-choywmwd	353	11	identified	identify	VBN
cord-104279-choywmwd	353	12	as	as	IN
cord-104279-choywmwd	353	13	the	the	DT
cord-104279-choywmwd	353	14	DPAP	DPAP	NNP
cord-104279-choywmwd	353	15	A	a	DT
cord-104279-choywmwd	353	16	Golgi	Golgi	NNP
cord-104279-choywmwd	353	17	retention	retention	NN
cord-104279-choywmwd	353	18	signal	signal	NN
cord-104279-choywmwd	353	19	contains	contain	VBZ
cord-104279-choywmwd	353	20	five	five	CD
cord-104279-choywmwd	353	21	phenylalanine	phenylalanine	NN
cord-104279-choywmwd	353	22	residues	residue	NNS
cord-104279-choywmwd	353	23	(	(	-LRB-
cord-104279-choywmwd	353	24	Fig	Fig	NNP
cord-104279-choywmwd	353	25	.	.	NNP
cord-104279-choywmwd	353	26	1	1	CD
cord-104279-choywmwd	353	27	)	)	-RRB-
cord-104279-choywmwd	353	28	.	.	.
cord-104279-choywmwd	354	1	In	in	IN
cord-104279-choywmwd	354	2	animal	animal	NN
cord-104279-choywmwd	354	3	cells	cell	NNS
cord-104279-choywmwd	354	4	,	,	,
cord-104279-choywmwd	354	5	one	one	CD
cord-104279-choywmwd	354	6	or	or	CC
cord-104279-choywmwd	354	7	more	more	JJR
cord-104279-choywmwd	354	8	aromatic	aromatic	JJ
cord-104279-choywmwd	354	9	amino	amino	NN
cord-104279-choywmwd	354	10	acids	acid	NNS
cord-104279-choywmwd	354	11	in	in	IN
cord-104279-choywmwd	354	12	the	the	DT
cord-104279-choywmwd	354	13	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	354	14	domains	domain	NNS
cord-104279-choywmwd	354	15	of	of	IN
cord-104279-choywmwd	354	16	cell	cell	NN
cord-104279-choywmwd	354	17	surface	surface	NN
cord-104279-choywmwd	354	18	receptors	receptor	NNS
cord-104279-choywmwd	354	19	have	have	VBP
cord-104279-choywmwd	354	20	been	be	VBN
cord-104279-choywmwd	354	21	shown	show	VBN
cord-104279-choywmwd	354	22	to	to	TO
cord-104279-choywmwd	354	23	comprise	comprise	VB
cord-104279-choywmwd	354	24	part	part	NN
cord-104279-choywmwd	354	25	of	of	IN
cord-104279-choywmwd	354	26	the	the	DT
cord-104279-choywmwd	354	27	signal	signal	NN
cord-104279-choywmwd	354	28	for	for	IN
cord-104279-choywmwd	354	29	the	the	DT
cord-104279-choywmwd	354	30	clustering	clustering	NN
cord-104279-choywmwd	354	31	into	into	IN
cord-104279-choywmwd	354	32	coated	coated	JJ
cord-104279-choywmwd	354	33	pit	pit	NN
cord-104279-choywmwd	354	34	regions	region	NNS
cord-104279-choywmwd	354	35	of	of	IN
cord-104279-choywmwd	354	36	the	the	DT
cord-104279-choywmwd	354	37	plasma	plasma	NN
cord-104279-choywmwd	354	38	membrane	membrane	NN
cord-104279-choywmwd	355	1	(	(	-LRB-
cord-104279-choywmwd	355	2	Chen	Chen	NNP
cord-104279-choywmwd	355	3	et	et	NNP
cord-104279-choywmwd	355	4	al	al	NNP
cord-104279-choywmwd	355	5	.	.	NNP
cord-104279-choywmwd	355	6	,	,	,
cord-104279-choywmwd	355	7	1990	1990	CD
cord-104279-choywmwd	355	8	;	;	:
cord-104279-choywmwd	355	9	Johnson	Johnson	NNP
cord-104279-choywmwd	355	10	et	et	NNP
cord-104279-choywmwd	355	11	al	al	NNP
cord-104279-choywmwd	355	12	.	.	NNP
cord-104279-choywmwd	355	13	,	,	,
cord-104279-choywmwd	355	14	1990	1990	CD
cord-104279-choywmwd	355	15	;	;	:
cord-104279-choywmwd	356	1	Lobel	Lobel	NNP
cord-104279-choywmwd	356	2	et	et	NNP
cord-104279-choywmwd	356	3	al	al	NNP
cord-104279-choywmwd	356	4	.	.	NNP
cord-104279-choywmwd	356	5	,	,	,
cord-104279-choywmwd	356	6	1989	1989	CD
cord-104279-choywmwd	356	7	;	;	:
cord-104279-choywmwd	357	1	McGraw	McGraw	NNP
cord-104279-choywmwd	357	2	et	et	NNP
cord-104279-choywmwd	357	3	al	al	NNP
cord-104279-choywmwd	357	4	.	.	NNP
cord-104279-choywmwd	357	5	,	,	,
cord-104279-choywmwd	357	6	1991	1991	CD
cord-104279-choywmwd	357	7	)	)	-RRB-
cord-104279-choywmwd	357	8	.	.	.
cord-104279-choywmwd	358	1	That	that	IN
cord-104279-choywmwd	358	2	the	the	DT
cord-104279-choywmwd	358	3	phenylalanine	phenylalanine	NN
cord-104279-choywmwd	358	4	residues	residue	NNS
cord-104279-choywmwd	358	5	in	in	IN
cord-104279-choywmwd	358	6	the	the	DT
cord-104279-choywmwd	358	7	A22	A22	NNP
cord-104279-choywmwd	358	8	region	region	NN
cord-104279-choywmwd	358	9	may	may	MD
cord-104279-choywmwd	358	10	play	play	VB
cord-104279-choywmwd	358	11	a	a	DT
cord-104279-choywmwd	358	12	direct	direct	JJ
cord-104279-choywmwd	358	13	role	role	NN
cord-104279-choywmwd	358	14	in	in	IN
cord-104279-choywmwd	358	15	Golgi	Golgi	NNP
cord-104279-choywmwd	358	16	retention	retention	NN
cord-104279-choywmwd	358	17	is	be	VBZ
cord-104279-choywmwd	358	18	supported	support	VBN
cord-104279-choywmwd	358	19	by	by	IN
cord-104279-choywmwd	358	20	the	the	DT
cord-104279-choywmwd	358	21	observation	observation	NN
cord-104279-choywmwd	358	22	that	that	WDT
cord-104279-choywmwd	358	23	mutations	mutation	NNS
cord-104279-choywmwd	358	24	in	in	IN
cord-104279-choywmwd	358	25	just	just	RB
cord-104279-choywmwd	358	26	two	two	CD
cord-104279-choywmwd	358	27	of	of	IN
cord-104279-choywmwd	358	28	these	these	DT
cord-104279-choywmwd	358	29	residues	residue	NNS
cord-104279-choywmwd	358	30	result	result	VBP
cord-104279-choywmwd	358	31	in	in	IN
cord-104279-choywmwd	358	32	a	a	DT
cord-104279-choywmwd	358	33	substantial	substantial	JJ
cord-104279-choywmwd	358	34	level	level	NN
cord-104279-choywmwd	358	35	of	of	IN
cord-104279-choywmwd	358	36	missorting	missorting	NN
cord-104279-choywmwd	358	37	of	of	IN
cord-104279-choywmwd	358	38	DPAP	DPAP	NNP
cord-104279-choywmwd	358	39	A	A	NNP
cord-104279-choywmwd	358	40	to	to	IN
cord-104279-choywmwd	358	41	the	the	DT
cord-104279-choywmwd	358	42	vacuole	vacuole	NN
cord-104279-choywmwd	358	43	(	(	-LRB-
cord-104279-choywmwd	358	44	S.	S.	NNP
cord-104279-choywmwd	358	45	Nothwehr	Nothwehr	NNP
cord-104279-choywmwd	358	46	and	and	CC
cord-104279-choywmwd	358	47	T.	T.	NNP
cord-104279-choywmwd	358	48	Stevens	Stevens	NNP
cord-104279-choywmwd	358	49	,	,	,
cord-104279-choywmwd	358	50	unpublished	unpublished	JJ
cord-104279-choywmwd	358	51	data	datum	NNS
cord-104279-choywmwd	358	52	)	)	-RRB-
cord-104279-choywmwd	358	53	.	.	.
cord-104279-choywmwd	359	1	The	the	DT
cord-104279-choywmwd	359	2	results	result	NNS
cord-104279-choywmwd	359	3	of	of	IN
cord-104279-choywmwd	359	4	this	this	DT
cord-104279-choywmwd	359	5	study	study	NN
cord-104279-choywmwd	359	6	suggest	suggest	VBP
cord-104279-choywmwd	359	7	that	that	IN
cord-104279-choywmwd	359	8	the	the	DT
cord-104279-choywmwd	359	9	default	default	NN
cord-104279-choywmwd	359	10	pathway	pathway	NN
cord-104279-choywmwd	359	11	for	for	IN
cord-104279-choywmwd	359	12	membrane	membrane	NN
cord-104279-choywmwd	359	13	proteins	protein	NNS
cord-104279-choywmwd	359	14	of	of	IN
cord-104279-choywmwd	359	15	the	the	DT
cord-104279-choywmwd	359	16	yeast	yeast	NN
cord-104279-choywmwd	359	17	secretory	secretory	NN
cord-104279-choywmwd	359	18	pathway	pathway	NN
cord-104279-choywmwd	359	19	may	may	MD
cord-104279-choywmwd	359	20	be	be	VB
cord-104279-choywmwd	359	21	different	different	JJ
cord-104279-choywmwd	359	22	from	from	IN
cord-104279-choywmwd	359	23	that	that	DT
cord-104279-choywmwd	359	24	of	of	IN
cord-104279-choywmwd	359	25	certain	certain	JJ
cord-104279-choywmwd	359	26	mammalian	mammalian	JJ
cord-104279-choywmwd	359	27	cell	cell	NN
cord-104279-choywmwd	359	28	lines	line	NNS
cord-104279-choywmwd	359	29	that	that	WDT
cord-104279-choywmwd	359	30	have	have	VBP
cord-104279-choywmwd	359	31	been	be	VBN
cord-104279-choywmwd	359	32	examined	examine	VBN
cord-104279-choywmwd	359	33	,	,	,
cord-104279-choywmwd	359	34	where	where	WRB
cord-104279-choywmwd	359	35	mutant	mutant	JJ
cord-104279-choywmwd	359	36	forms	form	NNS
cord-104279-choywmwd	359	37	of	of	IN
cord-104279-choywmwd	359	38	ER	ER	NNP
cord-104279-choywmwd	359	39	,	,	,
cord-104279-choywmwd	359	40	Golgi	Golgi	NNP
cord-104279-choywmwd	359	41	,	,	,
cord-104279-choywmwd	359	42	and	and	CC
cord-104279-choywmwd	359	43	lysosomal	lysosomal	JJ
cord-104279-choywmwd	359	44	membrane	membrane	NN
cord-104279-choywmwd	359	45	proteins	protein	NNS
cord-104279-choywmwd	359	46	are	be	VBP
cord-104279-choywmwd	359	47	mislocalized	mislocalize	VBN
cord-104279-choywmwd	359	48	to	to	IN
cord-104279-choywmwd	359	49	the	the	DT
cord-104279-choywmwd	359	50	plasma	plasma	NN
cord-104279-choywmwd	359	51	membrane	membrane	NN
cord-104279-choywmwd	359	52	with	with	IN
cord-104279-choywmwd	359	53	the	the	DT
cord-104279-choywmwd	359	54	bulk	bulk	JJ
cord-104279-choywmwd	359	55	flow	flow	NN
cord-104279-choywmwd	359	56	of	of	IN
cord-104279-choywmwd	359	57	membrane	membrane	NN
cord-104279-choywmwd	359	58	(	(	-LRB-
cord-104279-choywmwd	359	59	Machamer	Machamer	NNP
cord-104279-choywmwd	359	60	and	and	CC
cord-104279-choywmwd	359	61	Rose	Rose	NNP
cord-104279-choywmwd	359	62	,	,	,
cord-104279-choywmwd	359	63	1987	1987	CD
cord-104279-choywmwd	359	64	;	;	:
cord-104279-choywmwd	359	65	Jackson	Jackson	NNP
cord-104279-choywmwd	359	66	et	et	NNP
cord-104279-choywmwd	359	67	al	al	NNP
cord-104279-choywmwd	359	68	.	.	NNP
cord-104279-choywmwd	359	69	,	,	,
cord-104279-choywmwd	359	70	1990	1990	CD
cord-104279-choywmwd	359	71	;	;	:
cord-104279-choywmwd	359	72	Williams	Williams	NNP
cord-104279-choywmwd	359	73	and	and	CC
cord-104279-choywmwd	359	74	Fukuda	Fukuda	NNP
cord-104279-choywmwd	359	75	,	,	,
cord-104279-choywmwd	359	76	1990	1990	CD
cord-104279-choywmwd	359	77	;	;	:
cord-104279-choywmwd	359	78	Wieland	Wieland	NNP
cord-104279-choywmwd	359	79	et	et	NNP
cord-104279-choywmwd	359	80	al	al	NNP
cord-104279-choywmwd	359	81	.	.	NNP
cord-104279-choywmwd	359	82	,	,	,
cord-104279-choywmwd	359	83	1987	1987	CD
cord-104279-choywmwd	359	84	;	;	:
cord-104279-choywmwd	359	85	Karrenbauer	Karrenbauer	NNP
cord-104279-choywmwd	359	86	et	et	NNP
cord-104279-choywmwd	359	87	al	al	NNP
cord-104279-choywmwd	359	88	.	.	NNP
cord-104279-choywmwd	359	89	,	,	,
cord-104279-choywmwd	359	90	1990	1990	CD
cord-104279-choywmwd	359	91	;	;	:
cord-104279-choywmwd	359	92	Machamer	Machamer	NNP
cord-104279-choywmwd	359	93	,	,	,
cord-104279-choywmwd	359	94	1991	1991	CD
cord-104279-choywmwd	359	95	)	)	-RRB-
cord-104279-choywmwd	359	96	.	.	.
cord-104279-choywmwd	360	1	However	however	RB
cord-104279-choywmwd	360	2	,	,	,
cord-104279-choywmwd	360	3	in	in	IN
cord-104279-choywmwd	360	4	polarized	polarized	JJ
cord-104279-choywmwd	360	5	epithelial	epithelial	JJ
cord-104279-choywmwd	360	6	ceils	ceil	NNS
cord-104279-choywmwd	360	7	,	,	,
cord-104279-choywmwd	360	8	it	-PRON-	PRP
cord-104279-choywmwd	360	9	remains	remain	VBZ
cord-104279-choywmwd	360	10	unclear	unclear	JJ
cord-104279-choywmwd	360	11	which	which	WDT
cord-104279-choywmwd	360	12	membrane	membrane	NN
cord-104279-choywmwd	360	13	serves	serve	VBZ
cord-104279-choywmwd	360	14	as	as	IN
cord-104279-choywmwd	360	15	the	the	DT
cord-104279-choywmwd	360	16	default	default	NN
cord-104279-choywmwd	360	17	destination	destination	NN
cord-104279-choywmwd	360	18	for	for	IN
cord-104279-choywmwd	360	19	membrane	membrane	NN
cord-104279-choywmwd	360	20	proteins	protein	NNS
cord-104279-choywmwd	360	21	(	(	-LRB-
cord-104279-choywmwd	360	22	Simons	Simons	NNP
cord-104279-choywmwd	360	23	and	and	CC
cord-104279-choywmwd	360	24	Wandinger	Wandinger	NNP
cord-104279-choywmwd	360	25	-	-	HYPH
cord-104279-choywmwd	360	26	Ness	Ness	NNP
cord-104279-choywmwd	360	27	,	,	,
cord-104279-choywmwd	360	28	1990	1990	CD
cord-104279-choywmwd	360	29	;	;	:
cord-104279-choywmwd	361	1	Mostov	Mostov	NNP
cord-104279-choywmwd	361	2	et	et	NNP
cord-104279-choywmwd	361	3	al	al	NNP
cord-104279-choywmwd	361	4	.	.	NNP
cord-104279-choywmwd	361	5	,	,	,
cord-104279-choywmwd	361	6	1992	1992	CD
cord-104279-choywmwd	361	7	)	)	-RRB-
cord-104279-choywmwd	361	8	.	.	.
cord-104279-choywmwd	362	1	Even	even	RB
cord-104279-choywmwd	362	2	if	if	IN
cord-104279-choywmwd	362	3	the	the	DT
cord-104279-choywmwd	362	4	default	default	NN
cord-104279-choywmwd	362	5	compartments	compartment	NNS
cord-104279-choywmwd	362	6	for	for	IN
cord-104279-choywmwd	362	7	membrane	membrane	NN
cord-104279-choywmwd	362	8	proteins	protein	NNS
cord-104279-choywmwd	362	9	of	of	IN
cord-104279-choywmwd	362	10	the	the	DT
cord-104279-choywmwd	362	11	secretory	secretory	JJ
cord-104279-choywmwd	362	12	pathways	pathway	NNS
cord-104279-choywmwd	362	13	of	of	IN
cord-104279-choywmwd	362	14	yeast	yeast	NN
cord-104279-choywmwd	362	15	and	and	CC
cord-104279-choywmwd	362	16	certain	certain	JJ
cord-104279-choywmwd	362	17	animal	animal	NN
cord-104279-choywmwd	362	18	cells	cell	NNS
cord-104279-choywmwd	362	19	are	be	VBP
cord-104279-choywmwd	362	20	different	different	JJ
cord-104279-choywmwd	362	21	,	,	,
cord-104279-choywmwd	362	22	the	the	DT
cord-104279-choywmwd	362	23	same	same	JJ
cord-104279-choywmwd	362	24	mechanistic	mechanistic	JJ
cord-104279-choywmwd	362	25	considerations	consideration	NNS
cord-104279-choywmwd	362	26	apply	apply	VBP
cord-104279-choywmwd	362	27	,	,	,
cord-104279-choywmwd	362	28	that	that	RB
cord-104279-choywmwd	362	29	is	is	RB
cord-104279-choywmwd	362	30	,	,	,
cord-104279-choywmwd	362	31	positive	positive	JJ
cord-104279-choywmwd	362	32	sorting	sorting	NN
cord-104279-choywmwd	362	33	information	information	NN
cord-104279-choywmwd	362	34	is	be	VBZ
cord-104279-choywmwd	362	35	required	require	VBN
cord-104279-choywmwd	362	36	for	for	IN
cord-104279-choywmwd	362	37	proteins	protein	NNS
cord-104279-choywmwd	362	38	to	to	TO
cord-104279-choywmwd	362	39	avoid	avoid	VB
cord-104279-choywmwd	362	40	delivery	delivery	NN
cord-104279-choywmwd	362	41	to	to	IN
cord-104279-choywmwd	362	42	the	the	DT
cord-104279-choywmwd	362	43	default	default	NN
cord-104279-choywmwd	362	44	compartment	compartment	NN
cord-104279-choywmwd	362	45	.	.	.
cord-104279-choywmwd	363	1	According	accord	VBG
cord-104279-choywmwd	363	2	to	to	IN
cord-104279-choywmwd	363	3	the	the	DT
cord-104279-choywmwd	363	4	vacuolar	vacuolar	NNP
cord-104279-choywmwd	363	5	default	default	NN
cord-104279-choywmwd	363	6	model	model	NN
cord-104279-choywmwd	363	7	for	for	IN
cord-104279-choywmwd	363	8	yeast	yeast	NN
cord-104279-choywmwd	363	9	membrane	membrane	NN
cord-104279-choywmwd	363	10	protein	protein	NN
cord-104279-choywmwd	363	11	sorting	sorting	NN
cord-104279-choywmwd	363	12	,	,	,
cord-104279-choywmwd	363	13	not	not	RB
cord-104279-choywmwd	363	14	only	only	RB
cord-104279-choywmwd	363	15	must	must	MD
cord-104279-choywmwd	363	16	ER	ER	NNP
cord-104279-choywmwd	363	17	and	and	CC
cord-104279-choywmwd	363	18	Golgi	Golgi	NNP
cord-104279-choywmwd	363	19	membrane	membrane	NN
cord-104279-choywmwd	363	20	proteins	protein	NNS
cord-104279-choywmwd	363	21	have	have	VBP
cord-104279-choywmwd	363	22	sorting	sorting	NN
cord-104279-choywmwd	363	23	signals	signal	NNS
cord-104279-choywmwd	363	24	specifying	specify	VBG
cord-104279-choywmwd	363	25	their	-PRON-	PRP$
cord-104279-choywmwd	363	26	retention	retention	NN
cord-104279-choywmwd	363	27	in	in	IN
cord-104279-choywmwd	363	28	the	the	DT
cord-104279-choywmwd	363	29	appropriate	appropriate	JJ
cord-104279-choywmwd	363	30	compartment	compartment	NN
cord-104279-choywmwd	363	31	,	,	,
cord-104279-choywmwd	363	32	but	but	CC
cord-104279-choywmwd	363	33	plasma	plasma	NN
cord-104279-choywmwd	363	34	membrane	membrane	NN
cord-104279-choywmwd	363	35	proteins	protein	NNS
cord-104279-choywmwd	363	36	are	be	VBP
cord-104279-choywmwd	363	37	predicted	predict	VBN
cord-104279-choywmwd	363	38	to	to	TO
cord-104279-choywmwd	363	39	have	have	VB
cord-104279-choywmwd	363	40	sorting	sort	VBG
cord-104279-choywmwd	363	41	information	information	NN
cord-104279-choywmwd	363	42	that	that	WDT
cord-104279-choywmwd	363	43	prevents	prevent	VBZ
cord-104279-choywmwd	363	44	their	-PRON-	PRP$
cord-104279-choywmwd	363	45	localization	localization	NN
cord-104279-choywmwd	363	46	to	to	IN
cord-104279-choywmwd	363	47	the	the	DT
cord-104279-choywmwd	363	48	vacuole	vacuole	NN
cord-104279-choywmwd	363	49	.	.	.
cord-104279-choywmwd	364	1	We	-PRON-	PRP
cord-104279-choywmwd	364	2	are	be	VBP
cord-104279-choywmwd	364	3	currently	currently	RB
cord-104279-choywmwd	364	4	investigating	investigate	VBG
cord-104279-choywmwd	364	5	whether	whether	IN
cord-104279-choywmwd	364	6	yeast	yeast	NN
cord-104279-choywmwd	364	7	plasma	plasma	NN
cord-104279-choywmwd	364	8	membrane	membrane	NN
cord-104279-choywmwd	364	9	proteins	protein	NNS
cord-104279-choywmwd	364	10	have	have	VBP
cord-104279-choywmwd	364	11	targeting	target	VBG
cord-104279-choywmwd	364	12	signals	signal	NNS
cord-104279-choywmwd	364	13	that	that	WDT
cord-104279-choywmwd	364	14	prevent	prevent	VBP
cord-104279-choywmwd	364	15	their	-PRON-	PRP$
cord-104279-choywmwd	364	16	transport	transport	NN
cord-104279-choywmwd	364	17	to	to	IN
cord-104279-choywmwd	364	18	the	the	DT
cord-104279-choywmwd	364	19	vacuole	vacuole	NN
cord-104279-choywmwd	364	20	.	.	.
cord-104279-choywmwd	365	1	Lysosomal	lysosomal	JJ
cord-104279-choywmwd	365	2	sorting	sort	VBG
cord-104279-choywmwd	365	3	mutants	mutant	NNS
cord-104279-choywmwd	365	4	of	of	IN
cord-104279-choywmwd	365	5	coronavirus	coronavirus	NN
cord-104279-choywmwd	365	6	E	e	NN
cord-104279-choywmwd	365	7	1	1	CD
cord-104279-choywmwd	365	8	protein	protein	NN
cord-104279-choywmwd	365	9	,	,	,
cord-104279-choywmwd	365	10	a	a	DT
cord-104279-choywmwd	365	11	Golgi	Golgi	NNP
cord-104279-choywmwd	365	12	membrane	membrane	NN
cord-104279-choywmwd	365	13	protein	protein	NN
cord-104279-choywmwd	365	14	Yeast	Yeast	NNP
cord-104279-choywmwd	365	15	KRE	KRE	NNP
cord-104279-choywmwd	365	16	genes	gene	NNS
cord-104279-choywmwd	365	17	provide	provide	VBP
cord-104279-choywmwd	365	18	evidence	evidence	NN
cord-104279-choywmwd	365	19	for	for	IN
cord-104279-choywmwd	365	20	a	a	DT
cord-104279-choywmwd	365	21	pathway	pathway	NN
cord-104279-choywmwd	365	22	of	of	IN
cord-104279-choywmwd	365	23	cell	cell	NN
cord-104279-choywmwd	365	24	wall/~	wall/~	CD
cord-104279-choywmwd	365	25	glucan	glucan	NN
cord-104279-choywmwd	365	26	assembly	assembly	NN
cord-104279-choywmwd	365	27	Constitutive	Constitutive	NNP
cord-104279-choywmwd	365	28	and	and	CC
cord-104279-choywmwd	365	29	regulated	regulated	JJ
cord-104279-choywmwd	365	30	secretion	secretion	NN
cord-104279-choywmwd	365	31	of	of	IN
cord-104279-choywmwd	365	32	proteins	protein	NNS
cord-104279-choywmwd	365	33	Proteases	Proteases	NNP
cord-104279-choywmwd	365	34	and	and	CC
cord-104279-choywmwd	365	35	the	the	DT
cord-104279-choywmwd	365	36	processing	processing	NN
cord-104279-choywmwd	365	37	of	of	IN
cord-104279-choywmwd	365	38	precursors	precursor	NNS
cord-104279-choywmwd	365	39	to	to	IN
cord-104279-choywmwd	365	40	secreted	secrete	VBN
cord-104279-choywmwd	365	41	proteins	protein	NNS
cord-104279-choywmwd	365	42	in	in	IN
cord-104279-choywmwd	365	43	yeast	yeast	NN
cord-104279-choywmwd	365	44	NPXY	NPXY	NNP
cord-104279-choywmwd	365	45	,	,	,
cord-104279-choywmwd	365	46	a	a	DT
cord-104279-choywmwd	365	47	sequence	sequence	NN
cord-104279-choywmwd	365	48	often	often	RB
cord-104279-choywmwd	365	49	found	find	VBN
cord-104279-choywmwd	365	50	in	in	IN
cord-104279-choywmwd	365	51	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	365	52	tails	tail	NNS
cord-104279-choywmwd	365	53	,	,	,
cord-104279-choywmwd	365	54	is	be	VBZ
cord-104279-choywmwd	365	55	required	require	VBN
cord-104279-choywmwd	365	56	for	for	IN
cord-104279-choywmwd	365	57	coated	coated	JJ
cord-104279-choywmwd	365	58	pit	pit	NN
cord-104279-choywmwd	365	59	mediated	mediate	VBN
cord-104279-choywmwd	365	60	internalization	internalization	NN
cord-104279-choywmwd	365	61	of	of	IN
cord-104279-choywmwd	365	62	the	the	DT
cord-104279-choywmwd	365	63	LDL	LDL	NNP
cord-104279-choywmwd	365	64	receptor	receptor	NN
cord-104279-choywmwd	365	65	Characterization	characterization	NN
cord-104279-choywmwd	365	66	of	of	IN
cord-104279-choywmwd	365	67	the	the	DT
cord-104279-choywmwd	365	68	yeast	yeast	NN
cord-104279-choywmwd	365	69	KEX1	KEX1	NNP
cord-104279-choywmwd	365	70	gene	gene	NN
cord-104279-choywmwd	365	71	product	product	NN
cord-104279-choywmwd	365	72	"	"	''
cord-104279-choywmwd	365	73	a	a	DT
cord-104279-choywmwd	365	74	carboxypeptidase	carboxypeptidase	NN
cord-104279-choywmwd	365	75	involved	involve	VBN
cord-104279-choywmwd	365	76	in	in	IN
cord-104279-choywmwd	365	77	processing	process	VBG
cord-104279-choywmwd	365	78	secreted	secrete	VBN
cord-104279-choywmwd	365	79	precursor	precursor	NN
cord-104279-choywmwd	365	80	proteins	protein	NNS
cord-104279-choywmwd	365	81	Differential	differential	JJ
cord-104279-choywmwd	365	82	effects	effect	NNS
cord-104279-choywmwd	365	83	of	of	IN
cord-104279-choywmwd	365	84	mutations	mutation	NNS
cord-104279-choywmwd	365	85	in	in	IN
cord-104279-choywmwd	365	86	three	three	CD
cord-104279-choywmwd	365	87	domains	domain	NNS
cord-104279-choywmwd	365	88	on	on	IN
cord-104279-choywmwd	365	89	folding	fold	VBG
cord-104279-choywmwd	365	90	,	,	,
cord-104279-choywmwd	365	91	quaternary	quaternary	JJ
cord-104279-choywmwd	365	92	structure	structure	NN
cord-104279-choywmwd	365	93	,	,	,
cord-104279-choywmwd	365	94	and	and	CC
cord-104279-choywmwd	365	95	intracellular	intracellular	JJ
cord-104279-choywmwd	365	96	transport	transport	NN
cord-104279-choywmwd	365	97	of	of	IN
cord-104279-choywmwd	365	98	vesicular	vesicular	JJ
cord-104279-choywmwd	365	99	stomatitis	stomatitis	NN
cord-104279-choywmwd	365	100	virus	virus	NN
cord-104279-choywmwd	365	101	G	g	NN
cord-104279-choywmwd	365	102	protein	protein	NN
cord-104279-choywmwd	366	1	An	an	DT
cord-104279-choywmwd	366	2	MFod	MFod	NNP
cord-104279-choywmwd	366	3	-	-	HYPH
cord-104279-choywmwd	366	4	SUC2	SUC2	NNP
cord-104279-choywmwd	366	5	(	(	-LRB-
cord-104279-choywmwd	366	6	~-factor	~-factor	NNP
cord-104279-choywmwd	366	7	-	-	HYPH
cord-104279-choywmwd	366	8	invertase	invertase	NNP
cord-104279-choywmwd	366	9	)	)	-RRB-
cord-104279-choywmwd	366	10	gene	gene	NN
cord-104279-choywmwd	366	11	fusion	fusion	NN
cord-104279-choywmwd	366	12	for	for	IN
cord-104279-choywmwd	366	13	study	study	NN
cord-104279-choywmwd	366	14	of	of	IN
cord-104279-choywmwd	366	15	protein	protein	NN
cord-104279-choywmwd	366	16	localization	localization	NN
cord-104279-choywmwd	366	17	and	and	CC
cord-104279-choywmwd	366	18	gene	gene	NN
cord-104279-choywmwd	366	19	expression	expression	NN
cord-104279-choywmwd	366	20	in	in	IN
cord-104279-choywmwd	366	21	yeast	yeast	NN
cord-104279-choywmwd	367	1	The	the	DT
cord-104279-choywmwd	367	2	amino	amino	NN
cord-104279-choywmwd	367	3	terminus	terminus	NN
cord-104279-choywmwd	367	4	of	of	IN
cord-104279-choywmwd	367	5	the	the	DT
cord-104279-choywmwd	367	6	yeast	yeast	NN
cord-104279-choywmwd	367	7	Ft	Ft	NNP
cord-104279-choywmwd	367	8	-	-	HYPH
cord-104279-choywmwd	367	9	ATPase	ATPase	NNP
cord-104279-choywmwd	367	10	~-subunit	~-subunit	NNP
cord-104279-choywmwd	367	11	precursor	precursor	NN
cord-104279-choywmwd	367	12	functions	function	NNS
cord-104279-choywmwd	367	13	as	as	IN
cord-104279-choywmwd	367	14	a	a	DT
cord-104279-choywmwd	367	15	mitochondrial	mitochondrial	JJ
cord-104279-choywmwd	367	16	import	import	NN
cord-104279-choywmwd	367	17	signal	signal	NN
cord-104279-choywmwd	367	18	Compartmentalized	compartmentalize	VBN
cord-104279-choywmwd	367	19	assembly	assembly	NN
cord-104279-choywmwd	367	20	of	of	IN
cord-104279-choywmwd	367	21	oligosaccharides	oligosaccharide	NNS
cord-104279-choywmwd	367	22	on	on	IN
cord-104279-choywmwd	367	23	exported	export	VBN
cord-104279-choywmwd	367	24	glycoprotein	glycoprotein	NN
cord-104279-choywmwd	367	25	in	in	IN
cord-104279-choywmwd	367	26	yeast	yeast	NN
cord-104279-choywmwd	367	27	Localization	Localization	NNP
cord-104279-choywmwd	367	28	of	of	IN
cord-104279-choywmwd	367	29	components	component	NNS
cord-104279-choywmwd	367	30	involved	involve	VBN
cord-104279-choywmwd	367	31	in	in	IN
cord-104279-choywmwd	367	32	protein	protein	NN
cord-104279-choywmwd	367	33	transport	transport	NN
cord-104279-choywmwd	367	34	and	and	CC
cord-104279-choywmwd	367	35	processing	process	VBG
cord-104279-choywmwd	367	36	through	through	IN
cord-104279-choywmwd	367	37	the	the	DT
cord-104279-choywmwd	367	38	yeast	yeast	NN
cord-104279-choywmwd	367	39	Golgi	Golgi	NNP
cord-104279-choywmwd	367	40	apparatus	apparatus	NN
cord-104279-choywmwd	367	41	Enzymes	enzyme	NNS
cord-104279-choywmwd	367	42	required	require	VBN
cord-104279-choywmwd	367	43	for	for	IN
cord-104279-choywmwd	367	44	yeast	yeast	NN
cord-104279-choywmwd	367	45	prohormone	prohormone	NN
cord-104279-choywmwd	367	46	processing	process	VBG
cord-104279-choywmwd	367	47	Yeast	yeast	NN
cord-104279-choywmwd	367	48	prohormone	prohormone	NN
cord-104279-choywmwd	367	49	processing	processing	NN
cord-104279-choywmwd	367	50	enzyme	enzyme	NN
cord-104279-choywmwd	367	51	(	(	-LRB-
cord-104279-choywmwd	367	52	KE	KE	NNP
cord-104279-choywmwd	367	53	,	,	,
cord-104279-choywmwd	367	54	X2	x2	NN
cord-104279-choywmwd	367	55	gene	gene	NN
cord-104279-choywmwd	367	56	product	product	NN
cord-104279-choywmwd	367	57	)	)	-RRB-
cord-104279-choywmwd	367	58	is	be	VBZ
cord-104279-choywmwd	367	59	a	a	DT
cord-104279-choywmwd	367	60	Ca~+-dependent	Ca~+-dependent	NNP
cord-104279-choywmwd	367	61	serine	serine	NN
cord-104279-choywmwd	367	62	protease	protease	NN
cord-104279-choywmwd	367	63	Intracellular	Intracellular	NNP
cord-104279-choywmwd	367	64	targeting	target	VBG
cord-104279-choywmwd	367	65	and	and	CC
cord-104279-choywmwd	367	66	structural	structural	JJ
cord-104279-choywmwd	367	67	conservation	conservation	NN
cord-104279-choywmwd	367	68	of	of	IN
cord-104279-choywmwd	367	69	a	a	DT
cord-104279-choywmwd	367	70	prohormone	prohormone	NN
cord-104279-choywmwd	367	71	-	-	HYPH
cord-104279-choywmwd	367	72	processing	processing	NN
cord-104279-choywmwd	367	73	endoprotease	endoprotease	NN
cord-104279-choywmwd	367	74	Expression	expression	NN
cord-104279-choywmwd	367	75	of	of	IN
cord-104279-choywmwd	367	76	wild	wild	JJ
cord-104279-choywmwd	367	77	type	type	NN
cord-104279-choywmwd	367	78	and	and	CC
cord-104279-choywmwd	367	79	mutant	mutant	JJ
cord-104279-choywmwd	367	80	forms	form	NNS
cord-104279-choywmwd	367	81	of	of	IN
cord-104279-choywmwd	367	82	influenza	influenza	NN
cord-104279-choywmwd	367	83	hemagglutinin	hemagglutinin	NN
cord-104279-choywmwd	367	84	:	:	:
cord-104279-choywmwd	367	85	the	the	DT
cord-104279-choywmwd	367	86	role	role	NN
cord-104279-choywmwd	367	87	of	of	IN
cord-104279-choywmwd	367	88	folding	fold	VBG
cord-104279-choywmwd	367	89	in	in	IN
cord-104279-choywmwd	367	90	intracellular	intracellular	JJ
cord-104279-choywmwd	367	91	transport	transport	NN
cord-104279-choywmwd	367	92	/3-D	/3-D	NNP
cord-104279-choywmwd	367	93	-	-	HYPH
cord-104279-choywmwd	367	94	fructofuranoside	fructofuranoside	NN
cord-104279-choywmwd	367	95	fructohydrolase	fructohydrolase	NN
cord-104279-choywmwd	367	96	from	from	IN
cord-104279-choywmwd	367	97	yeast	yeast	NN
cord-104279-choywmwd	367	98	Compartmental	Compartmental	NNP
cord-104279-choywmwd	367	99	organization	organization	NN
cord-104279-choywmwd	367	100	of	of	IN
cord-104279-choywmwd	367	101	Golgispecific	Golgispecific	NNP
cord-104279-choywmwd	367	102	protein	protein	NN
cord-104279-choywmwd	367	103	modification	modification	NN
cord-104279-choywmwd	367	104	and	and	CC
cord-104279-choywmwd	367	105	vacuolar	vacuolar	NNP
cord-104279-choywmwd	367	106	protein	protein	NN
cord-104279-choywmwd	367	107	sorting	sort	VBG
cord-104279-choywmwd	367	108	events	event	NNS
cord-104279-choywmwd	367	109	defined	define	VBN
cord-104279-choywmwd	367	110	in	in	IN
cord-104279-choywmwd	367	111	a	a	DT
cord-104279-choywmwd	367	112	yeast	yeast	NN
cord-104279-choywmwd	367	113	secl8	secl8	NN
cord-104279-choywmwd	367	114	(	(	-LRB-
cord-104279-choywmwd	367	115	NSF	NSF	NNP
cord-104279-choywmwd	367	116	)	)	-RRB-
cord-104279-choywmwd	367	117	mutant	mutant	JJ
cord-104279-choywmwd	367	118	ERDI	ERDI	NNP
cord-104279-choywmwd	367	119	,	,	,
cord-104279-choywmwd	367	120	a	a	DT
cord-104279-choywmwd	367	121	yeast	yeast	NN
cord-104279-choywmwd	367	122	gene	gene	NN
cord-104279-choywmwd	367	123	required	require	VBN
cord-104279-choywmwd	367	124	for	for	IN
cord-104279-choywmwd	367	125	the	the	DT
cord-104279-choywmwd	367	126	retention	retention	NN
cord-104279-choywmwd	367	127	of	of	IN
cord-104279-choywmwd	367	128	luminal	luminal	JJ
cord-104279-choywmwd	367	129	endoplasmic	endoplasmic	NN
cord-104279-choywmwd	367	130	reticulum	reticulum	NN
cord-104279-choywmwd	367	131	proteins	protein	NNS
cord-104279-choywmwd	367	132	,	,	,
cord-104279-choywmwd	367	133	affects	affect	VBZ
cord-104279-choywmwd	367	134	glycoprotein	glycoprotein	NN
cord-104279-choywmwd	367	135	processing	processing	NN
cord-104279-choywmwd	367	136	in	in	IN
cord-104279-choywmwd	367	137	the	the	DT
cord-104279-choywmwd	367	138	Golgi	Golgi	NNP
cord-104279-choywmwd	367	139	apparatus	apparatus	NN
cord-104279-choywmwd	367	140	Solid	solid	JJ
cord-104279-choywmwd	367	141	phase	phase	NN
cord-104279-choywmwd	367	142	synthesis	synthesis	NN
cord-104279-choywmwd	367	143	of	of	IN
cord-104279-choywmwd	367	144	polynucleotides	polynucleotides	NNP
cord-104279-choywmwd	367	145	VI	VI	NNP
cord-104279-choywmwd	367	146	.	.	.
cord-104279-choywmwd	368	1	Further	further	JJ
cord-104279-choywmwd	368	2	studies	study	NNS
cord-104279-choywmwd	368	3	on	on	IN
cord-104279-choywmwd	368	4	polystyrene	polystyrene	NN
cord-104279-choywmwd	368	5	copolymers	copolymer	NNS
cord-104279-choywmwd	368	6	for	for	IN
cord-104279-choywmwd	368	7	the	the	DT
cord-104279-choywmwd	368	8	solid	solid	JJ
cord-104279-choywmwd	368	9	support	support	NN
cord-104279-choywmwd	368	10	Transformation	transformation	NN
cord-104279-choywmwd	368	11	of	of	IN
cord-104279-choywmwd	368	12	intact	intact	JJ
cord-104279-choywmwd	368	13	yeast	yeast	NN
cord-104279-choywmwd	368	14	ceils	ceil	NNS
cord-104279-choywmwd	368	15	treated	treat	VBN
cord-104279-choywmwd	368	16	with	with	IN
cord-104279-choywmwd	368	17	alkali	alkali	JJ
cord-104279-choywmwd	368	18	cations	cation	NNS
cord-104279-choywmwd	368	19	Identification	identification	NN
cord-104279-choywmwd	368	20	of	of	IN
cord-104279-choywmwd	368	21	a	a	DT
cord-104279-choywmwd	368	22	consensus	consensus	NN
cord-104279-choywmwd	368	23	motif	motif	NN
cord-104279-choywmwd	368	24	for	for	IN
cord-104279-choywmwd	368	25	retention	retention	NN
cord-104279-choywmwd	368	26	of	of	IN
cord-104279-choywmwd	368	27	transmembrane	transmembrane	JJ
cord-104279-choywmwd	368	28	proteins	protein	NNS
cord-104279-choywmwd	368	29	in	in	IN
cord-104279-choywmwd	368	30	the	the	DT
cord-104279-choywmwd	368	31	endoplasmic	endoplasmic	NN
cord-104279-choywmwd	368	32	reticulum	reticulum	NN
cord-104279-choywmwd	368	33	Cation	Cation	NNP
cord-104279-choywmwd	368	34	-	-	HYPH
cord-104279-choywmwd	368	35	dependent	dependent	JJ
cord-104279-choywmwd	368	36	mannose	mannose	NN
cord-104279-choywmwd	368	37	6-pbosphate	6-pbosphate	CD
cord-104279-choywmwd	368	38	receptor	receptor	NN
cord-104279-choywmwd	368	39	contains	contain	VBZ
cord-104279-choywmwd	368	40	two	two	CD
cord-104279-choywmwd	368	41	internalization	internalization	NN
cord-104279-choywmwd	368	42	signals	signal	NNS
cord-104279-choywmwd	368	43	in	in	IN
cord-104279-choywmwd	368	44	its	-PRON-	PRP$
cord-104279-choywmwd	368	45	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	368	46	domain	domain	NN
cord-104279-choywmwd	368	47	Distinct	distinct	JJ
cord-104279-choywmwd	368	48	sequence	sequence	NN
cord-104279-choywmwd	368	49	determinants	determinant	VBZ
cord-104279-choywmwd	368	50	direct	direct	JJ
cord-104279-choywmwd	368	51	intracellular	intracellular	JJ
cord-104279-choywmwd	368	52	sorting	sorting	NN
cord-104279-choywmwd	368	53	and	and	CC
cord-104279-choywmwd	368	54	modification	modification	NN
cord-104279-choywmwd	368	55	of	of	IN
cord-104279-choywmwd	368	56	a	a	DT
cord-104279-choywmwd	368	57	yeast	yeast	NN
cord-104279-choywmwd	368	58	vacuolar	vacuolar	NN
cord-104279-choywmwd	368	59	proteinase	proteinase	NN
cord-104279-choywmwd	368	60	Sequences	Sequences	NNPS
cord-104279-choywmwd	368	61	that	that	WDT
cord-104279-choywmwd	368	62	regulate	regulate	VBP
cord-104279-choywmwd	368	63	the	the	DT
cord-104279-choywmwd	368	64	divergent	divergent	JJ
cord-104279-choywmwd	368	65	GAL1-GALIO	gal1-galio	NN
cord-104279-choywmwd	368	66	promoter	promoter	NN
cord-104279-choywmwd	368	67	in	in	IN
cord-104279-choywmwd	368	68	Saccharomyces	Saccharomyces	NNP
cord-104279-choywmwd	368	69	cerevisiae	cerevisiae	NNS
cord-104279-choywmwd	368	70	Yeast	yeast	VB
cord-104279-choywmwd	368	71	a	a	DT
cord-104279-choywmwd	368	72	factor	factor	NN
cord-104279-choywmwd	368	73	is	be	VBZ
cord-104279-choywmwd	368	74	processed	process	VBN
cord-104279-choywmwd	368	75	from	from	IN
cord-104279-choywmwd	368	76	a	a	DT
cord-104279-choywmwd	368	77	larger	large	JJR
cord-104279-choywmwd	368	78	precursor	precursor	NN
cord-104279-choywmwd	368	79	polypeptide	polypeptide	NN
cord-104279-choywmwd	368	80	:	:	:
cord-104279-choywmwd	368	81	the	the	DT
cord-104279-choywmwd	368	82	essential	essential	JJ
cord-104279-choywmwd	368	83	role	role	NN
cord-104279-choywmwd	368	84	of	of	IN
cord-104279-choywmwd	368	85	a	a	DT
cord-104279-choywmwd	368	86	membrane	membrane	NN
cord-104279-choywmwd	368	87	-	-	HYPH
cord-104279-choywmwd	368	88	bound	bind	VBN
cord-104279-choywmwd	368	89	dipeptidyl	dipeptidyl	JJ
cord-104279-choywmwd	368	90	aminopeptidase	aminopeptidase	NN
cord-104279-choywmwd	368	91	Glycosylation	Glycosylation	NNP
cord-104279-choywmwd	368	92	and	and	CC
cord-104279-choywmwd	368	93	processing	processing	NN
cord-104279-choywmwd	368	94	of	of	IN
cord-104279-choywmwd	368	95	prepro	prepro	NN
cord-104279-choywmwd	368	96	-	-	HYPH
cord-104279-choywmwd	368	97	a	a	DT
cord-104279-choywmwd	368	98	-	-	HYPH
cord-104279-choywmwd	368	99	factor	factor	NN
cord-104279-choywmwd	368	100	through	through	IN
cord-104279-choywmwd	368	101	the	the	DT
cord-104279-choywmwd	368	102	yeast	yeast	NN
cord-104279-choywmwd	368	103	secretory	secretory	NN
cord-104279-choywmwd	368	104	pathway	pathway	NN
cord-104279-choywmwd	368	105	Assembly	Assembly	NNP
cord-104279-choywmwd	368	106	and	and	CC
cord-104279-choywmwd	368	107	targeting	targeting	NN
cord-104279-choywmwd	368	108	of	of	IN
cord-104279-choywmwd	368	109	peripheral	peripheral	JJ
cord-104279-choywmwd	368	110	and	and	CC
cord-104279-choywmwd	368	111	membrane	membrane	NN
cord-104279-choywmwd	368	112	subunits	subunit	NNS
cord-104279-choywmwd	368	113	of	of	IN
cord-104279-choywmwd	368	114	the	the	DT
cord-104279-choywmwd	368	115	yeast	yeast	NN
cord-104279-choywmwd	368	116	vacuolar	vacuolar	NNP
cord-104279-choywmwd	369	1	H+-ATPase	h+-atpase	NN
cord-104279-choywmwd	370	1	The	the	DT
cord-104279-choywmwd	370	2	rate	rate	NN
cord-104279-choywmwd	370	3	of	of	IN
cord-104279-choywmwd	370	4	bulk	bulk	JJ
cord-104279-choywmwd	370	5	flow	flow	NN
cord-104279-choywmwd	370	6	from	from	IN
cord-104279-choywmwd	370	7	the	the	DT
cord-104279-choywmwd	370	8	Golgi	Golgi	NNP
cord-104279-choywmwd	370	9	to	to	IN
cord-104279-choywmwd	370	10	the	the	DT
cord-104279-choywmwd	370	11	plasma	plasma	NN
cord-104279-choywmwd	370	12	membrane	membrane	NN
cord-104279-choywmwd	370	13	Protein	Protein	NNP
cord-104279-choywmwd	370	14	degradation	degradation	NN
cord-104279-choywmwd	370	15	in	in	IN
cord-104279-choywmwd	370	16	the	the	DT
cord-104279-choywmwd	370	17	endoplasmic	endoplasmic	NN
cord-104279-choywmwd	370	18	reticulum	reticulum	NN
cord-104279-choywmwd	370	19	Membrane	Membrane	NNP
cord-104279-choywmwd	370	20	protein	protein	NN
cord-104279-choywmwd	370	21	sorting	sorting	NN
cord-104279-choywmwd	370	22	:	:	:
cord-104279-choywmwd	370	23	biosynthesis	biosynthesis	NN
cord-104279-choywmwd	370	24	,	,	,
cord-104279-choywmwd	370	25	transport	transport	NN
cord-104279-choywmwd	370	26	,	,	,
cord-104279-choywmwd	370	27	and	and	CC
cord-104279-choywmwd	370	28	processing	processing	NN
cord-104279-choywmwd	370	29	of	of	IN
cord-104279-choywmwd	370	30	yeast	yeast	NN
cord-104279-choywmwd	370	31	vacuolar	vacuolar	NNP
cord-104279-choywmwd	370	32	alkaline	alkaline	JJ
cord-104279-choywmwd	370	33	phosphatase	phosphatase	NN
cord-104279-choywmwd	370	34	A	a	DT
cord-104279-choywmwd	370	35	new	new	JJ
cord-104279-choywmwd	370	36	class	class	NN
cord-104279-choywmwd	370	37	of	of	IN
cord-104279-choywmwd	370	38	lysosomal	lysosomal	JJ
cord-104279-choywmwd	370	39	/	/	SYM
cord-104279-choywmwd	370	40	vacuolar	vacuolar	NNP
cord-104279-choywmwd	370	41	protein	protein	NN
cord-104279-choywmwd	370	42	sorting	sorting	NN
cord-104279-choywmwd	370	43	signals	signal	NNS
cord-104279-choywmwd	370	44	Protein	protein	NN
cord-104279-choywmwd	370	45	glycosylation	glycosylation	NN
cord-104279-choywmwd	370	46	in	in	IN
cord-104279-choywmwd	370	47	yeast	yeast	NN
cord-104279-choywmwd	370	48	Rapid	Rapid	NNP
cord-104279-choywmwd	370	49	and	and	CC
cord-104279-choywmwd	370	50	efficient	efficient	JJ
cord-104279-choywmwd	370	51	sitespecific	sitespecific	NN
cord-104279-choywmwd	370	52	mutagenesis	mutagenesis	NN
cord-104279-choywmwd	370	53	without	without	IN
cord-104279-choywmwd	370	54	phenotypic	phenotypic	NN
cord-104279-choywmwd	370	55	selection	selection	NN
cord-104279-choywmwd	370	56	Structure	Structure	NNP
cord-104279-choywmwd	370	57	of	of	IN
cord-104279-choywmwd	370	58	a	a	DT
cord-104279-choywmwd	370	59	yeast	yeast	NN
cord-104279-choywmwd	370	60	pheromone	pheromone	NN
cord-104279-choywmwd	370	61	gene	gene	NN
cord-104279-choywmwd	370	62	(	(	-LRB-
cord-104279-choywmwd	370	63	MFa	mfa	NN
cord-104279-choywmwd	370	64	)	)	-RRB-
cord-104279-choywmwd	370	65	:	:	:
cord-104279-choywmwd	370	66	a	a	DT
cord-104279-choywmwd	370	67	putative	putative	JJ
cord-104279-choywmwd	370	68	a	a	DT
cord-104279-choywmwd	370	69	-	-	HYPH
cord-104279-choywmwd	370	70	factor	factor	NN
cord-104279-choywmwd	370	71	precursor	precursor	NN
cord-104279-choywmwd	370	72	contains	contain	VBZ
cord-104279-choywmwd	370	73	four	four	CD
cord-104279-choywmwd	370	74	tandem	tandem	NN
cord-104279-choywmwd	370	75	copies	copy	NNS
cord-104279-choywmwd	370	76	of	of	IN
cord-104279-choywmwd	370	77	mature	mature	JJ
cord-104279-choywmwd	370	78	c~-factor	c~-factor	NN
cord-104279-choywmwd	370	79	Mutations	mutation	NNS
cord-104279-choywmwd	370	80	in	in	IN
cord-104279-choywmwd	370	81	the	the	DT
cord-104279-choywmwd	370	82	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	370	83	domain	domain	NN
cord-104279-choywmwd	370	84	of	of	IN
cord-104279-choywmwd	370	85	the	the	DT
cord-104279-choywmwd	370	86	275	275	CD
cord-104279-choywmwd	370	87	kd	kd	NNP
cord-104279-choywmwd	370	88	mannose	mannose	VBG
cord-104279-choywmwd	370	89	6-phosphate	6-phosphate	NN
cord-104279-choywmwd	370	90	receptor	receptor	NN
cord-104279-choywmwd	370	91	differentially	differentially	RB
cord-104279-choywmwd	370	92	alter	alter	VBP
cord-104279-choywmwd	370	93	lysosomal	lysosomal	JJ
cord-104279-choywmwd	370	94	enzyme	enzyme	NN
cord-104279-choywmwd	370	95	sorting	sorting	NN
cord-104279-choywmwd	370	96	and	and	CC
cord-104279-choywmwd	370	97	endoeytosis	endoeytosis	NNP
cord-104279-choywmwd	370	98	Golgi	Golgi	NNP
cord-104279-choywmwd	370	99	retention	retention	NN
cord-104279-choywmwd	370	100	signals	signal	NNS
cord-104279-choywmwd	370	101	:	:	:
cord-104279-choywmwd	370	102	do	do	VBP
cord-104279-choywmwd	370	103	membranes	membrane	NNS
cord-104279-choywmwd	370	104	hold	hold	VB
cord-104279-choywmwd	370	105	the	the	DT
cord-104279-choywmwd	370	106	key	key	NN
cord-104279-choywmwd	370	107	?	?	.
cord-104279-choywmwd	371	1	A	a	DT
cord-104279-choywmwd	371	2	specific	specific	JJ
cord-104279-choywmwd	371	3	transmembrane	transmembrane	NN
cord-104279-choywmwd	371	4	domain	domain	NN
cord-104279-choywmwd	371	5	of	of	IN
cord-104279-choywmwd	371	6	a	a	DT
cord-104279-choywmwd	371	7	coronavirus	coronavirus	NN
cord-104279-choywmwd	371	8	El	El	NNP
cord-104279-choywmwd	371	9	glycoprotein	glycoprotein	NN
cord-104279-choywmwd	371	10	is	be	VBZ
cord-104279-choywmwd	371	11	required	require	VBN
cord-104279-choywmwd	371	12	for	for	IN
cord-104279-choywmwd	371	13	its	-PRON-	PRP$
cord-104279-choywmwd	371	14	retention	retention	NN
cord-104279-choywmwd	371	15	in	in	IN
cord-104279-choywmwd	371	16	the	the	DT
cord-104279-choywmwd	371	17	Golgi	Golgi	NNP
cord-104279-choywmwd	371	18	region	region	NN
cord-104279-choywmwd	371	19	Molecular	Molecular	NNP
cord-104279-choywmwd	371	20	Cloning	Cloning	NNP
cord-104279-choywmwd	371	21	:	:	:
cord-104279-choywmwd	371	22	A	a	DT
cord-104279-choywmwd	371	23	Laboratory	Laboratory	NNP
cord-104279-choywmwd	371	24	Manual	Manual	NNP
cord-104279-choywmwd	371	25	.	.	.
cord-104279-choywmwd	372	1	Cold	Cold	NNP
cord-104279-choywmwd	372	2	Spring	Spring	NNP
cord-104279-choywmwd	372	3	Harbor	Harbor	NNP
cord-104279-choywmwd	372	4	Laboratory	Laboratory	NNP
cord-104279-choywmwd	372	5	Identification	Identification	NNP
cord-104279-choywmwd	372	6	and	and	CC
cord-104279-choywmwd	372	7	regulation	regulation	NN
cord-104279-choywmwd	372	8	of	of	IN
cord-104279-choywmwd	372	9	a	a	DT
cord-104279-choywmwd	372	10	gene	gene	NN
cord-104279-choywmwd	372	11	required	require	VBN
cord-104279-choywmwd	372	12	for	for	IN
cord-104279-choywmwd	372	13	cell	cell	NN
cord-104279-choywmwd	372	14	fusion	fusion	NN
cord-104279-choywmwd	372	15	during	during	IN
cord-104279-choywmwd	372	16	mating	mating	NN
cord-104279-choywmwd	372	17	of	of	IN
cord-104279-choywmwd	372	18	the	the	DT
cord-104279-choywmwd	372	19	yeast	yeast	NN
cord-104279-choywmwd	372	20	Saccharomyces	saccharomyce	NNS
cord-104279-choywmwd	372	21	cerevisiae	cerevisiae	VBZ
cord-104279-choywmwd	372	22	Mutaganesis	Mutaganesis	NNP
cord-104279-choywmwd	372	23	of	of	IN
cord-104279-choywmwd	372	24	the	the	DT
cord-104279-choywmwd	372	25	human	human	JJ
cord-104279-choywmwd	372	26	transferrin	transferrin	NN
cord-104279-choywmwd	372	27	receptor	receptor	NN
cord-104279-choywmwd	372	28	:	:	:
cord-104279-choywmwd	372	29	two	two	CD
cord-104279-choywmwd	372	30	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	372	31	phenylalanines	phenylalanine	NNS
cord-104279-choywmwd	372	32	are	be	VBP
cord-104279-choywmwd	372	33	required	require	VBN
cord-104279-choywmwd	372	34	for	for	IN
cord-104279-choywmwd	372	35	efficient	efficient	JJ
cord-104279-choywmwd	372	36	internalization	internalization	NN
cord-104279-choywmwd	372	37	and	and	CC
cord-104279-choywmwd	372	38	a	a	DT
cord-104279-choywmwd	372	39	second	second	JJ
cord-104279-choywmwd	372	40	-	-	HYPH
cord-104279-choywmwd	372	41	site	site	NN
cord-104279-choywmwd	372	42	mutation	mutation	NN
cord-104279-choywmwd	372	43	is	be	VBZ
cord-104279-choywmwd	372	44	capable	capable	JJ
cord-104279-choywmwd	372	45	of	of	IN
cord-104279-choywmwd	372	46	reverting	revert	VBG
cord-104279-choywmwd	372	47	an	an	DT
cord-104279-choywmwd	372	48	internalization	internalization	NN
cord-104279-choywmwd	372	49	-	-	HYPH
cord-104279-choywmwd	372	50	defective	defective	JJ
cord-104279-choywmwd	372	51	phenotype	phenotype	NN
cord-104279-choywmwd	372	52	Building	build	VBG
cord-104279-choywmwd	372	53	a	a	DT
cord-104279-choywmwd	372	54	multichain	multichain	JJ
cord-104279-choywmwd	372	55	receptor	receptor	NN
cord-104279-choywmwd	372	56	:	:	:
cord-104279-choywmwd	372	57	synthesis	synthesis	NN
cord-104279-choywmwd	372	58	,	,	,
cord-104279-choywmwd	372	59	degradation	degradation	NN
cord-104279-choywmwd	372	60	and	and	CC
cord-104279-choywmwd	372	61	assembly	assembly	NN
cord-104279-choywmwd	372	62	of	of	IN
cord-104279-choywmwd	372	63	the	the	DT
cord-104279-choywmwd	372	64	T	T	NNP
cord-104279-choywmwd	372	65	-	-	HYPH
cord-104279-choywmwd	372	66	cell	cell	NN
cord-104279-choywmwd	372	67	antigen	antigen	NN
cord-104279-choywmwd	372	68	receptor	receptor	NN
cord-104279-choywmwd	372	69	Plasma	Plasma	NNP
cord-104279-choywmwd	372	70	membrane	membrane	NN
cord-104279-choywmwd	372	71	protein	protein	NN
cord-104279-choywmwd	372	72	sorting	sort	VBG
cord-104279-choywmwd	372	73	in	in	IN
cord-104279-choywmwd	372	74	polarized	polarized	JJ
cord-104279-choywmwd	372	75	epithelial	epithelial	JJ
cord-104279-choywmwd	372	76	cells	cell	NNS
cord-104279-choywmwd	372	77	Order	order	NN
cord-104279-choywmwd	372	78	of	of	IN
cord-104279-choywmwd	372	79	events	event	NNS
cord-104279-choywmwd	372	80	in	in	IN
cord-104279-choywmwd	372	81	the	the	DT
cord-104279-choywmwd	372	82	yeast	yeast	NN
cord-104279-choywmwd	372	83	secretory	secretory	NN
cord-104279-choywmwd	372	84	pathway	pathway	NN
cord-104279-choywmwd	372	85	Analysis	Analysis	NNP
cord-104279-choywmwd	372	86	ofglycoproteins	ofglycoprotein	NNS
cord-104279-choywmwd	372	87	from	from	IN
cord-104279-choywmwd	372	88	Saccharomyces	Saccharomyces	NNP
cord-104279-choywmwd	372	89	cerevisiae	cerevisiae	VBZ
cord-104279-choywmwd	372	90	Clathrin	Clathrin	NNP
cord-104279-choywmwd	372	91	:	:	:
cord-104279-choywmwd	373	1	a	a	DT
cord-104279-choywmwd	373	2	role	role	NN
cord-104279-choywmwd	373	3	in	in	IN
cord-104279-choywmwd	373	4	the	the	DT
cord-104279-choywmwd	373	5	intracellular	intracellular	JJ
cord-104279-choywmwd	373	6	retention	retention	NN
cord-104279-choywmwd	373	7	of	of	IN
cord-104279-choywmwd	373	8	a	a	DT
cord-104279-choywmwd	373	9	Golgi	Golgi	NNP
cord-104279-choywmwd	373	10	membrane	membrane	NN
cord-104279-choywmwd	373	11	protein	protein	NN
cord-104279-choywmwd	373	12	Control	Control	NNP
cord-104279-choywmwd	373	13	of	of	IN
cord-104279-choywmwd	373	14	protein	protein	NN
cord-104279-choywmwd	373	15	exit	exit	NN
cord-104279-choywmwd	373	16	from	from	IN
cord-104279-choywmwd	373	17	the	the	DT
cord-104279-choywmwd	373	18	endoplasmic	endoplasmic	NN
cord-104279-choywmwd	373	19	reticulum	reticulum	NN
cord-104279-choywmwd	373	20	Biosynthetic	biosynthetic	JJ
cord-104279-choywmwd	373	21	protein	protein	NN
cord-104279-choywmwd	373	22	transport	transport	NN
cord-104279-choywmwd	373	23	and	and	CC
cord-104279-choywmwd	373	24	sorting	sorting	NN
cord-104279-choywmwd	373	25	by	by	IN
cord-104279-choywmwd	373	26	the	the	DT
cord-104279-choywmwd	373	27	endoplasmic	endoplasmic	NN
cord-104279-choywmwd	373	28	reticulum	reticulum	NN
cord-104279-choywmwd	373	29	and	and	CC
cord-104279-choywmwd	373	30	Golgi	Golgi	NNP
cord-104279-choywmwd	373	31	Molecular	Molecular	NNP
cord-104279-choywmwd	373	32	analysis	analysis	NN
cord-104279-choywmwd	373	33	of	of	IN
cord-104279-choywmwd	373	34	the	the	DT
cord-104279-choywmwd	373	35	yeast	yeast	NN
cord-104279-choywmwd	373	36	VPS3	VPS3	NNP
cord-104279-choywmwd	373	37	gene	gene	NN
cord-104279-choywmwd	373	38	and	and	CC
cord-104279-choywmwd	373	39	the	the	DT
cord-104279-choywmwd	373	40	role	role	NN
cord-104279-choywmwd	373	41	of	of	IN
cord-104279-choywmwd	373	42	its	-PRON-	PRP$
cord-104279-choywmwd	373	43	product	product	NN
cord-104279-choywmwd	373	44	in	in	IN
cord-104279-choywmwd	373	45	vacuolar	vacuolar	NNP
cord-104279-choywmwd	373	46	protein	protein	NN
cord-104279-choywmwd	373	47	sorting	sorting	NN
cord-104279-choywmwd	373	48	and	and	CC
cord-104279-choywmwd	373	49	vacuolar	vacuolar	NNP
cord-104279-choywmwd	373	50	segregation	segregation	NN
cord-104279-choywmwd	373	51	during	during	IN
cord-104279-choywmwd	373	52	the	the	DT
cord-104279-choywmwd	373	53	cell	cell	NN
cord-104279-choywmwd	373	54	cycle	cycle	NN
cord-104279-choywmwd	373	55	Immunolocalization	Immunolocalization	NNP
cord-104279-choywmwd	373	56	of	of	IN
cord-104279-choywmwd	373	57	Kex2	Kex2	NNP
cord-104279-choywmwd	373	58	protease	protease	NN
cord-104279-choywmwd	373	59	identifies	identify	VBZ
cord-104279-choywmwd	373	60	a	a	DT
cord-104279-choywmwd	373	61	putative	putative	JJ
cord-104279-choywmwd	373	62	late	late	JJ
cord-104279-choywmwd	373	63	Golgi	Golgi	NNP
cord-104279-choywmwd	373	64	compartment	compartment	NN
cord-104279-choywmwd	373	65	in	in	IN
cord-104279-choywmwd	373	66	the	the	DT
cord-104279-choywmwd	373	67	yeast	yeast	NN
cord-104279-choywmwd	373	68	Saccharomyces	Saccharomyces	NNP
cord-104279-choywmwd	373	69	cerevisiae	cerevisiae	VBZ
cord-104279-choywmwd	373	70	Structure	structure	NN
cord-104279-choywmwd	373	71	,	,	,
cord-104279-choywmwd	373	72	biosynthesis	biosynthesis	NN
cord-104279-choywmwd	373	73	,	,	,
cord-104279-choywmwd	373	74	and	and	CC
cord-104279-choywmwd	373	75	localization	localization	NN
cord-104279-choywmwd	373	76	of	of	IN
cord-104279-choywmwd	373	77	dipeptidyl	dipeptidyl	JJ
cord-104279-choywmwd	373	78	aminopeptidase	aminopeptidase	NN
cord-104279-choywmwd	373	79	B	B	NNP
cord-104279-choywmwd	373	80	,	,	,
cord-104279-choywmwd	373	81	an	an	DT
cord-104279-choywmwd	373	82	integral	integral	JJ
cord-104279-choywmwd	373	83	membrane	membrane	NN
cord-104279-choywmwd	373	84	glycoprotein	glycoprotein	NN
cord-104279-choywmwd	373	85	of	of	IN
cord-104279-choywmwd	373	86	the	the	DT
cord-104279-choywmwd	373	87	yeast	yeast	NN
cord-104279-choywmwd	373	88	vacuole	vacuole	NN
cord-104279-choywmwd	373	89	Methods	method	NNS
cord-104279-choywmwd	373	90	for	for	IN
cord-104279-choywmwd	373	91	studying	study	VBG
cord-104279-choywmwd	373	92	the	the	DT
cord-104279-choywmwd	373	93	yeast	yeast	NN
cord-104279-choywmwd	373	94	vacuole	vacuole	NN
cord-104279-choywmwd	373	95	Cloning	clone	VBG
cord-104279-choywmwd	373	96	genes	gene	NNS
cord-104279-choywmwd	373	97	by	by	IN
cord-104279-choywmwd	373	98	complementation	complementation	NN
cord-104279-choywmwd	373	99	in	in	IN
cord-104279-choywmwd	373	100	yeast	yeast	NN
cord-104279-choywmwd	373	101	KAR2	KAR2	NNP
cord-104279-choywmwd	373	102	,	,	,
cord-104279-choywmwd	373	103	a	a	DT
cord-104279-choywmwd	373	104	karyogamy	karyogamy	NN
cord-104279-choywmwd	373	105	gene	gene	NN
cord-104279-choywmwd	373	106	,	,	,
cord-104279-choywmwd	373	107	is	be	VBZ
cord-104279-choywmwd	373	108	the	the	DT
cord-104279-choywmwd	373	109	yeast	yeast	NN
cord-104279-choywmwd	373	110	homolog	homolog	NN
cord-104279-choywmwd	373	111	of	of	IN
cord-104279-choywmwd	373	112	the	the	DT
cord-104279-choywmwd	373	113	mammalian	mammalian	JJ
cord-104279-choywmwd	373	114	BiP	bip	NN
cord-104279-choywmwd	373	115	/	/	,
cord-104279-choywmwd	373	116	GRP78	GRP78	NNP
cord-104279-choywmwd	373	117	gene	gene	NN
cord-104279-choywmwd	373	118	Polypeptide	Polypeptide	NNP
cord-104279-choywmwd	373	119	chain	chain	NN
cord-104279-choywmwd	373	120	binding	bind	VBG
cord-104279-choywmwd	373	121	proteins	protein	NNS
cord-104279-choywmwd	373	122	:	:	:
cord-104279-choywmwd	373	123	catalysts	catalyst	NNS
cord-104279-choywmwd	373	124	of	of	IN
cord-104279-choywmwd	373	125	protein	protein	NN
cord-104279-choywmwd	373	126	folding	folding	NN
cord-104279-choywmwd	373	127	and	and	CC
cord-104279-choywmwd	373	128	related	related	JJ
cord-104279-choywmwd	373	129	processes	process	NNS
cord-104279-choywmwd	373	130	in	in	IN
cord-104279-choywmwd	373	131	ceils	ceils	NNP
cord-104279-choywmwd	373	132	One	one	CD
cord-104279-choywmwd	373	133	step	step	NN
cord-104279-choywmwd	373	134	gene	gene	NN
cord-104279-choywmwd	373	135	disruption	disruption	NN
cord-104279-choywmwd	373	136	in	in	IN
cord-104279-choywmwd	373	137	yeast	yeast	NN
cord-104279-choywmwd	374	1	A	a	DT
cord-104279-choywmwd	374	2	ras	ras	NN
cord-104279-choywmwd	374	3	-	-	HYPH
cord-104279-choywmwd	374	4	like	like	JJ
cord-104279-choywmwd	374	5	protein	protein	NN
cord-104279-choywmwd	374	6	is	be	VBZ
cord-104279-choywmwd	374	7	required	require	VBN
cord-104279-choywmwd	374	8	for	for	IN
cord-104279-choywmwd	374	9	a	a	DT
cord-104279-choywmwd	374	10	post	post	JJ
cord-104279-choywmwd	374	11	-	-	JJ
cord-104279-choywmwd	374	12	Golgi	golgi	JJ
cord-104279-choywmwd	374	13	event	event	NN
cord-104279-choywmwd	374	14	in	in	IN
cord-104279-choywmwd	374	15	yeast	yeast	NN
cord-104279-choywmwd	374	16	secretion	secretion	NN
cord-104279-choywmwd	374	17	Selective	selective	JJ
cord-104279-choywmwd	374	18	and	and	CC
cord-104279-choywmwd	374	19	immediate	immediate	JJ
cord-104279-choywmwd	374	20	effects	effect	NNS
cord-104279-choywmwd	374	21	of	of	IN
cord-104279-choywmwd	374	22	clathrin	clathrin	NN
cord-104279-choywmwd	374	23	heavy	heavy	JJ
cord-104279-choywmwd	374	24	chain	chain	NN
cord-104279-choywmwd	374	25	mutations	mutation	NNS
cord-104279-choywmwd	374	26	on	on	IN
cord-104279-choywmwd	374	27	Golgi	Golgi	NNP
cord-104279-choywmwd	374	28	membrane	membrane	NN
cord-104279-choywmwd	374	29	protein	protein	NN
cord-104279-choywmwd	374	30	retention	retention	NN
cord-104279-choywmwd	374	31	in	in	IN
cord-104279-choywmwd	374	32	Saccharomyces	Saccharomyces	NNP
cord-104279-choywmwd	374	33	cerevisiae	cerevisiae	VBZ
cord-104279-choywmwd	374	34	A	a	DT
cord-104279-choywmwd	374	35	role	role	NN
cord-104279-choywmwd	374	36	for	for	IN
cord-104279-choywmwd	374	37	clathrin	clathrin	NN
cord-104279-choywmwd	374	38	in	in	IN
cord-104279-choywmwd	374	39	the	the	DT
cord-104279-choywmwd	374	40	sorting	sorting	NN
cord-104279-choywmwd	374	41	of	of	IN
cord-104279-choywmwd	374	42	vacuolar	vacuolar	JJ
cord-104279-choywmwd	374	43	proteins	protein	NNS
cord-104279-choywmwd	374	44	in	in	IN
cord-104279-choywmwd	374	45	the	the	DT
cord-104279-choywmwd	374	46	Golgi	Golgi	NNP
cord-104279-choywmwd	374	47	complex	complex	NN
cord-104279-choywmwd	374	48	of	of	IN
cord-104279-choywmwd	374	49	yeast	yeast	NN
cord-104279-choywmwd	374	50	Methods	method	NNS
cord-104279-choywmwd	374	51	in	in	IN
cord-104279-choywmwd	374	52	yeast	yeast	NN
cord-104279-choywmwd	374	53	genetics	genetics	NN
cord-104279-choywmwd	374	54	A	a	DT
cord-104279-choywmwd	374	55	system	system	NN
cord-104279-choywmwd	374	56	of	of	IN
cord-104279-choywmwd	374	57	shuttle	shuttle	NN
cord-104279-choywmwd	374	58	vectors	vector	NNS
cord-104279-choywmwd	374	59	and	and	CC
cord-104279-choywmwd	374	60	yeast	yeast	NN
cord-104279-choywmwd	374	61	host	host	NN
cord-104279-choywmwd	374	62	strains	strain	NNS
cord-104279-choywmwd	374	63	designed	design	VBN
cord-104279-choywmwd	374	64	for	for	IN
cord-104279-choywmwd	374	65	efficient	efficient	JJ
cord-104279-choywmwd	374	66	manipulation	manipulation	NN
cord-104279-choywmwd	374	67	of	of	IN
cord-104279-choywmwd	374	68	DNA	dna	NN
cord-104279-choywmwd	374	69	in	in	IN
cord-104279-choywmwd	374	70	Saccharomyces	Saccharomyces	NNP
cord-104279-choywmwd	374	71	cerevisiae	cerevisiae	VBZ
cord-104279-choywmwd	374	72	Polarized	polarized	JJ
cord-104279-choywmwd	374	73	sorting	sorting	NN
cord-104279-choywmwd	374	74	in	in	IN
cord-104279-choywmwd	374	75	epithelia	epithelium	NNS
cord-104279-choywmwd	375	1	The	the	DT
cord-104279-choywmwd	375	2	structure	structure	NN
cord-104279-choywmwd	375	3	and	and	CC
cord-104279-choywmwd	375	4	insertion	insertion	NN
cord-104279-choywmwd	375	5	of	of	IN
cord-104279-choywmwd	375	6	integral	integral	JJ
cord-104279-choywmwd	375	7	proteins	protein	NNS
cord-104279-choywmwd	375	8	in	in	IN
cord-104279-choywmwd	375	9	membranes	membrane	NNS
cord-104279-choywmwd	375	10	Gene	gene	NN
cord-104279-choywmwd	375	11	dosage	dosage	NN
cord-104279-choywmwd	375	12	-	-	HYPH
cord-104279-choywmwd	375	13	dependent	dependent	JJ
cord-104279-choywmwd	375	14	secretion	secretion	NN
cord-104279-choywmwd	375	15	of	of	IN
cord-104279-choywmwd	375	16	yeast	yeast	NN
cord-104279-choywmwd	375	17	vacuolar	vacuolar	NNP
cord-104279-choywmwd	375	18	carboxypeptidase	carboxypeptidase	NN
cord-104279-choywmwd	375	19	Y	Y	NNP
cord-104279-choywmwd	375	20	Identification	Identification	NNP
cord-104279-choywmwd	375	21	of	of	IN
cord-104279-choywmwd	375	22	the	the	DT
cord-104279-choywmwd	375	23	structural	structural	JJ
cord-104279-choywmwd	375	24	gene	gene	NN
cord-104279-choywmwd	375	25	for	for	IN
cord-104279-choywmwd	375	26	dipeptidyl	dipeptidyl	JJ
cord-104279-choywmwd	375	27	aminopeptidase	aminopeptidase	NNP
cord-104279-choywmwd	375	28	yscV	yscV	''
cord-104279-choywmwd	375	29	(	(	-LRB-
cord-104279-choywmwd	375	30	DAP2	DAP2	NNP
cord-104279-choywmwd	375	31	)	)	-RRB-
cord-104279-choywmwd	375	32	of	of	IN
cord-104279-choywmwd	375	33	Saccharomyces	Saccharomyces	NNP
cord-104279-choywmwd	375	34	cerevisiae	cerevisiae	NNS
cord-104279-choywmwd	376	1	The	the	DT
cord-104279-choywmwd	376	2	yeast	yeast	NN
cord-104279-choywmwd	376	3	cell	cell	NN
cord-104279-choywmwd	376	4	fusion	fusion	NN
cord-104279-choywmwd	376	5	protein	protein	NN
cord-104279-choywmwd	376	6	FUSI	FUSI	NNP
cord-104279-choywmwd	376	7	is	be	VBZ
cord-104279-choywmwd	376	8	O	o	NN
cord-104279-choywmwd	376	9	-	-	HYPH
cord-104279-choywmwd	376	10	glycosylated	glycosylate	VBN
cord-104279-choywmwd	376	11	and	and	CC
cord-104279-choywmwd	376	12	spans	span	VBZ
cord-104279-choywmwd	376	13	the	the	DT
cord-104279-choywmwd	376	14	plasma	plasma	NN
cord-104279-choywmwd	376	15	membrane	membrane	NN
cord-104279-choywmwd	376	16	Two	two	CD
cord-104279-choywmwd	376	17	genes	gene	NNS
cord-104279-choywmwd	376	18	required	require	VBN
cord-104279-choywmwd	376	19	for	for	IN
cord-104279-choywmwd	376	20	cell	cell	NN
cord-104279-choywmwd	376	21	fusion	fusion	NN
cord-104279-choywmwd	376	22	in	in	IN
cord-104279-choywmwd	376	23	yeast	yeast	NN
cord-104279-choywmwd	376	24	:	:	:
cord-104279-choywmwd	377	1	evidence	evidence	NN
cord-104279-choywmwd	377	2	of	of	IN
cord-104279-choywmwd	377	3	a	a	DT
cord-104279-choywmwd	377	4	pheromone	pheromone	NN
cord-104279-choywmwd	377	5	-	-	HYPH
cord-104279-choywmwd	377	6	induced	induce	VBN
cord-104279-choywmwd	377	7	surface	surface	NN
cord-104279-choywmwd	377	8	protein	protein	NN
cord-104279-choywmwd	377	9	Yeast	yeast	NN
cord-104279-choywmwd	377	10	carboxypeptidase	carboxypeptidase	NN
cord-104279-choywmwd	377	11	Y	Y	NNP
cord-104279-choywmwd	377	12	vacuolar	vacuolar	NN
cord-104279-choywmwd	377	13	targeting	target	VBG
cord-104279-choywmwd	377	14	signal	signal	NN
cord-104279-choywmwd	377	15	is	be	VBZ
cord-104279-choywmwd	377	16	defined	define	VBN
cord-104279-choywmwd	377	17	by	by	IN
cord-104279-choywmwd	377	18	four	four	CD
cord-104279-choywmwd	377	19	propeptide	propeptide	NN
cord-104279-choywmwd	377	20	amino	amino	NN
cord-104279-choywmwd	377	21	acids	acid	NNS
cord-104279-choywmwd	378	1	A	a	DT
cord-104279-choywmwd	378	2	family	family	NN
cord-104279-choywmwd	378	3	of	of	IN
cord-104279-choywmwd	378	4	yeast	yeast	NN
cord-104279-choywmwd	378	5	expression	expression	NN
cord-104279-choywmwd	378	6	vectors	vector	NNS
cord-104279-choywmwd	378	7	containing	contain	VBG
cord-104279-choywmwd	378	8	the	the	DT
cord-104279-choywmwd	378	9	phage	phage	NN
cord-104279-choywmwd	378	10	fl	fl	NNP
cord-104279-choywmwd	378	11	intergenic	intergenic	JJ
cord-104279-choywmwd	378	12	region	region	NN
cord-104279-choywmwd	378	13	Prepro	Prepro	NNP
cord-104279-choywmwd	378	14	-	-	HYPH
cord-104279-choywmwd	378	15	a	a	DT
cord-104279-choywmwd	378	16	-	-	HYPH
cord-104279-choywmwd	378	17	factor	factor	NN
cord-104279-choywmwd	378	18	has	have	VBZ
cord-104279-choywmwd	378	19	a	a	DT
cord-104279-choywmwd	378	20	cleavable	cleavable	JJ
cord-104279-choywmwd	378	21	signal	signal	NN
cord-104279-choywmwd	378	22	sequence	sequence	NN
cord-104279-choywmwd	379	1	The	the	DT
cord-104279-choywmwd	379	2	rate	rate	NN
cord-104279-choywmwd	379	3	of	of	IN
cord-104279-choywmwd	379	4	bulk	bulk	JJ
cord-104279-choywmwd	379	5	flow	flow	NN
cord-104279-choywmwd	379	6	from	from	IN
cord-104279-choywmwd	379	7	the	the	DT
cord-104279-choywmwd	379	8	endoplasmic	endoplasmic	NN
cord-104279-choywmwd	379	9	reticulum	reticulum	NN
cord-104279-choywmwd	379	10	to	to	IN
cord-104279-choywmwd	379	11	the	the	DT
cord-104279-choywmwd	379	12	cell	cell	NN
cord-104279-choywmwd	379	13	surface	surface	NN
cord-104279-choywmwd	379	14	Accumulation	accumulation	NN
cord-104279-choywmwd	379	15	of	of	IN
cord-104279-choywmwd	379	16	membrane	membrane	NN
cord-104279-choywmwd	379	17	glycoproteins	glycoprotein	NNS
cord-104279-choywmwd	379	18	in	in	IN
cord-104279-choywmwd	379	19	lysosomes	lysosome	NNS
cord-104279-choywmwd	379	20	requires	require	VBZ
cord-104279-choywmwd	379	21	a	a	DT
cord-104279-choywmwd	379	22	tyrosine	tyrosine	NN
cord-104279-choywmwd	379	23	residue	residue	NN
cord-104279-choywmwd	379	24	at	at	IN
cord-104279-choywmwd	379	25	a	a	DT
cord-104279-choywmwd	379	26	particular	particular	JJ
cord-104279-choywmwd	379	27	position	position	NN
cord-104279-choywmwd	379	28	in	in	IN
cord-104279-choywmwd	379	29	the	the	DT
cord-104279-choywmwd	379	30	cytoplasmic	cytoplasmic	JJ
cord-104279-choywmwd	379	31	tail	tail	NN
cord-104279-choywmwd	380	1	We	-PRON-	PRP
cord-104279-choywmwd	380	2	acknowledge	acknowledge	VBP
cord-104279-choywmwd	380	3	Cathy	Cathy	NNP
cord-104279-choywmwd	380	4	Flanagan	Flanagan	NNP
cord-104279-choywmwd	380	5	,	,	,
cord-104279-choywmwd	380	6	Jeremy	Jeremy	NNP
cord-104279-choywmwd	380	7	Thorner	Thorner	NNP
cord-104279-choywmwd	380	8	,	,	,
cord-104279-choywmwd	380	9	Antony	Antony	NNP
cord-104279-choywmwd	380	10	Cooper	Cooper	NNP
cord-104279-choywmwd	380	11	,	,	,
cord-104279-choywmwd	380	12	Howard	Howard	NNP
cord-104279-choywmwd	380	13	Bussey	Bussey	NNP
cord-104279-choywmwd	380	14	,	,	,
cord-104279-choywmwd	380	15	Celeste	Celeste	NNP
cord-104279-choywmwd	380	16	Wilcox	Wilcox	NNP
cord-104279-choywmwd	380	17	,	,	,
cord-104279-choywmwd	380	18	Robert	Robert	NNP
cord-104279-choywmwd	380	19	Fuller	Fuller	NNP
cord-104279-choywmwd	380	20	,	,	,
cord-104279-choywmwd	380	21	and	and	CC
cord-104279-choywmwd	380	22	Greg	Greg	NNP
cord-104279-choywmwd	380	23	Payne	Payne	NNP
cord-104279-choywmwd	380	24	for	for	IN
cord-104279-choywmwd	380	25	communication	communication	NN
cord-104279-choywmwd	380	26	of	of	IN
cord-104279-choywmwd	380	27	results	result	NNS
cord-104279-choywmwd	380	28	prior	prior	RB
cord-104279-choywmwd	380	29	to	to	IN
cord-104279-choywmwd	380	30	publication	publication	NN
cord-104279-choywmwd	380	31	;	;	:
cord-104279-choywmwd	380	32	Scott	Scott	NNP
cord-104279-choywmwd	380	33	Ernr	Ernr	NNP
cord-104279-choywmwd	380	34	,	,	,
cord-104279-choywmwd	380	35	Charlie	Charlie	NNP
cord-104279-choywmwd	380	36	Boone	Boone	NNP
cord-104279-choywmwd	380	37	,	,	,
cord-104279-choywmwd	380	38	and	and	CC
cord-104279-choywmwd	380	39	George	George	NNP
cord-104279-choywmwd	380	40	Sprague	Sprague	NNP
cord-104279-choywmwd	380	41	for	for	IN
cord-104279-choywmwd	380	42	plasmids	plasmid	NNS
cord-104279-choywmwd	380	43	;	;	:
cord-104279-choywmwd	380	44	Margaret	Margaret	NNP
cord-104279-choywmwd	380	45	Ho	Ho	NNP
cord-104279-choywmwd	380	46	and	and	CC
cord-104279-choywmwd	380	47	Joe	Joe	NNP
cord-104279-choywmwd	380	48	Horecka	Horecka	NNP
cord-104279-choywmwd	380	49	for	for	IN
cord-104279-choywmwd	380	50	help	help	NN
cord-104279-choywmwd	380	51	in	in	IN
cord-104279-choywmwd	380	52	the	the	DT
cord-104279-choywmwd	380	53	construction	construction	NN
cord-104279-choywmwd	380	54	of	of	IN
cord-104279-choywmwd	380	55	the	the	DT
cord-104279-choywmwd	380	56	A20-BB	A20-BB	NNP
cord-104279-choywmwd	380	57	and	and	CC
cord-104279-choywmwd	380	58	Fusl	Fusl	NNP
cord-104279-choywmwd	380	59	-	-	HYPH
cord-104279-choywmwd	380	60	LacZp	LacZp	NNP
cord-104279-choywmwd	380	61	constructs	construct	NNS
cord-104279-choywmwd	380	62	,	,	,
cord-104279-choywmwd	380	63	respectively	respectively	RB
cord-104279-choywmwd	380	64	;	;	:
cord-104279-choywmwd	380	65	and	and	CC
cord-104279-choywmwd	380	66	Jerry	Jerry	NNP
cord-104279-choywmwd	380	67	Gleason	Gleason	NNP
cord-104279-choywmwd	380	68	and	and	CC
cord-104279-choywmwd	380	69	Scan	Scan	NNP
cord-104279-choywmwd	380	70	Poston	Poston	NNP
cord-104279-choywmwd	380	71	for	for	IN
cord-104279-choywmwd	380	72	the	the	DT
cord-104279-choywmwd	380	73	photographic	photographic	JJ
cord-104279-choywmwd	380	74	work	work	NN
cord-104279-choywmwd	380	75	.	.	.
cord-104279-choywmwd	381	1	We	-PRON-	PRP
cord-104279-choywmwd	381	2	especially	especially	RB
cord-104279-choywmwd	381	3	thank	thank	VBP
cord-104279-choywmwd	381	4	Christopher	Christopher	NNP
cord-104279-choywmwd	381	5	Raymond	Raymond	NNP
cord-104279-choywmwd	381	6	for	for	IN
cord-104279-choywmwd	381	7	numerous	numerous	JJ
cord-104279-choywmwd	381	8	insightful	insightful	JJ
cord-104279-choywmwd	381	9	contributions	contribution	NNS
cord-104279-choywmwd	381	10	to	to	IN
cord-104279-choywmwd	381	11	this	this	DT
cord-104279-choywmwd	381	12	work	work	NN
cord-104279-choywmwd	381	13	,	,	,
cord-104279-choywmwd	381	14	and	and	CC
cord-104279-choywmwd	381	15	Nick	Nick	NNP
cord-104279-choywmwd	381	16	Davis	Davis	NNP
cord-104279-choywmwd	381	17	,	,	,
cord-104279-choywmwd	381	18	Charlie	Charlie	NNP
cord-104279-choywmwd	381	19	Boone	Boone	NNP
cord-104279-choywmwd	381	20	,	,	,
cord-104279-choywmwd	381	21	George	George	NNP
cord-104279-choywmwd	381	22	Sprague	Sprague	NNP
cord-104279-choywmwd	381	23	,	,	,
cord-104279-choywmwd	381	24	Antony	Antony	NNP
cord-104279-choywmwd	381	25	Cooper	Cooper	NNP
cord-104279-choywmwd	381	26	,	,	,
cord-104279-choywmwd	381	27	Carol	Carol	NNP
cord-104279-choywmwd	381	28	Vater	Vater	NNP
cord-104279-choywmwd	381	29	,	,	,
cord-104279-choywmwd	381	30	Cynthia	Cynthia	NNP
cord-104279-choywmwd	381	31	Bauerle	Bauerle	NNP
cord-104279-choywmwd	381	32	,	,	,
cord-104279-choywmwd	381	33	and	and	CC
cord-104279-choywmwd	381	34	Margaret	Margaret	NNP
cord-104279-choywmwd	381	35	Ho	Ho	NNP
cord-104279-choywmwd	381	36	for	for	IN
cord-104279-choywmwd	381	37	comments	comment	NNS
cord-104279-choywmwd	381	38	on	on	IN
cord-104279-choywmwd	381	39	the	the	DT
cord-104279-choywmwd	381	40	manuscript	manuscript	NN
cord-104279-choywmwd	381	41	.	.	.
cord-104279-choywmwd	382	1	Received	receive	VBN
cord-104279-choywmwd	382	2	for	for	IN
cord-104279-choywmwd	382	3	publication	publication	NN
cord-104279-choywmwd	382	4	10	10	CD
cord-104279-choywmwd	382	5	February	February	NNP
cord-104279-choywmwd	382	6	1992	1992	CD
cord-104279-choywmwd	382	7	and	and	CC
cord-104279-choywmwd	382	8	in	in	IN
cord-104279-choywmwd	382	9	revised	revise	VBN
cord-104279-choywmwd	382	10	form	form	NN
cord-104279-choywmwd	382	11	30	30	CD
cord-104279-choywmwd	382	12	June	June	NNP
cord-104279-choywmwd	382	13	1992	1992	CD
cord-104279-choywmwd	382	14	.	.	.