id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263678-z94utwbk	Fu, Li	A combination of mutations in the S1 part of the spike glycoprotein gene of coronavirus MHV-A59 abolishes demyelination	2004		.txt	text/plain	4682	248	50	The authors have previously shown that the spike (S) glycoprotein gene of MHV contains determinants of virulence, hepatitis, and demyelination. In the present study, the authors produced new recombinant viruses with each one of these S gene mutations by site-directed mutagenesis and targeted recombination and studied the effect of each individual mutation on the pathogenesis of the virus. The use of the virus with feline spike protein (fMHV) also provided a Coronavirus S gene demyelination determinants L Fu et al 43 useful method to select recombinant viruses against the background of parental viruses. Viral RNA persistence of all recombinant and parental viruses in mouse organs (brain, spinal cord and liver) was analyzed by reverse transcriptase L Fu et al Figure 3 Pathologic analysis of recombinant viruses during acute infection. Targeted recombination within the spike gene of murine coronavirus mouse hepatitis virus-A59: Q159 is a determinant of hepatotropism	./cache/cord-263678-z94utwbk.txt	./txt/cord-263678-z94utwbk.txt