id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-270473-5tok4mqk	Nanda, S. K.	Mitochondrial HSP70, HSP40, and HSP60 bind to the 3′ untranslated region of the Murine hepatitis virus genome	2003-09-19		.txt	text/plain	7102	384	53	We have previously shown that mitochondrial-aconitase binds specifically to the 3′ terminal 42 nucleotides of the Murine hepatitis virus (MHV) RNA along with three additional proteins of 70, 58 and 40 kDa to form a stable RNA-protein complex. The assays were done essentially as previously described [22] except that the partially purified post-mitochondrial lysates (approximately 3 µg total protein) were incubated at 22 • C with purified m-apo-aconitase (8-10 µg, graciously supplied by M.C. Kennedy, Gannon University, Erie, PA) and MHV-JHM 3 UTR RNA in ATPase buffer (20 mM Hepes, pH 7.4, 100 mM KCl, 5 mM MgCl 2 , 0.1% NP-40, 1 mM DTT, 1 mM ATP containing 2 µCi [γ-32 P]ATP plus cocktails of protease and phosphatase inhibitors). Gel supershift assays with anti-phosphotyrosine antibodies (Fig. 2B ) demonstrate that at least one of the proteins in the complex formed with MHV 3 (+)42 containing RNAs and m-aconitase, mtHSP70, HSP60, and HSP40 is tyrosine phosphorylated.	./cache/cord-270473-5tok4mqk.txt	./txt/cord-270473-5tok4mqk.txt