id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301942-ppa7gb95	Neuman, Benjamin W.	Ultrastructure of SARS-CoV, FIPV, and MHV Revealed by Electron Cryomicroscopy	2006		.txt	text/plain	1189	78	47	The current understanding of coronavirus ultrastructure relies heavily on transmission electron microscopy of negatively stained images. Each virus appeared approximately round in cryo-EM images, with a fringe of spikes protruding from the viral membrane and a region of lower density near the virion center ( Fig. 1A-B) . Spike-depleted SARS-CoV particles appeared similar to spike-depleted MHV particles in negative stain, but were produced in lower yield, not suitable for effective cryo-EM imaging. The arrangement of spike densities near the center of some particles approximates a rhombus, which would not be inconsistent with a paracrystalline organization of spikes as observed in the virions of pleomorphic arenavirus particles, 17 or a local hexagonal close-packing of structural proteins as observed in retroviral particles. Fine structure of influenza A virus observed by electron cryo-microscopy Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle Supramolecular organization of immature and mature murine leukemia virus revealed by electron cryo-microscopy: implications for retroviral assembly mechanisms	./cache/cord-301942-ppa7gb95.txt	./txt/cord-301942-ppa7gb95.txt