id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-351934-g7tgo5cn	Deming, Damon J.	MHV-A59 Orf1a Replicase Protein NSP7-NSP10 Processing in Replication	2006		.txt	text/plain	1353	95	62	authors: Deming, Damon J.; Graham, Rachel L.; Denison, Mark R.; Baric, Ralph S. Through use of an efficient MHV-A59 reverse genetics system, 6 we ablated each of the M pro cleavage sites associated with the nsp7-nsp10 cassette, and evaluated whether the mutated genome was capable of supporting a viable virus, and if so, characterized the M pro processing of the mutated protein, transcription function, and in vitro growth fitness. Surprisingly, the recovered virus did not revert to wild-type sequence at the nsp9/nsp10 M pro cleavage site, indicating that an as of yet unidentified mutation(s) has compensated for the virus's inability to properly process the nsp9-nsp10 precursor protein. Serial passage of this virus restored wild-type replication but did so without reverting the mutated cleavage site or the ability to process the nsp9-nsp10 protein. The data demonstrate that with the exception of cleavage between the nsp9 and nsp10 proteins, M pro processing of the nsp7-nsp10 cassette is essential in coronavirus RNA transcription and replication.	./cache/cord-351934-g7tgo5cn.txt	./txt/cord-351934-g7tgo5cn.txt