id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001921-73esrper	Lin, Cheng-Yung	Zebrafish and Medaka: new model organisms for modern biomedical research	2016-01-28		.txt	text/plain	7725	417	42	Studies on gene expression patterns, regulatory cis-elements identification, and gene functions can be facilitated by using zebrafish embryos via a number of techniques, including transgenesis, in vivo transient assay, overexpression by injection of mRNAs, knockdown by injection of morpholino oligonucleotides, knockout and gene editing by CRISPR/Cas9 system and mutagenesis. In addition, transgenic lines of model fish harboring a tissue-specific reporter have become a powerful tool for the study of biological sciences, since it is possible to visualize the dynamic expression of a specific gene in the transparent embryos. generated a transgenic zebrafish line huORFZ, which harbors the upstream open reading frame (uORF) from human CCAAT/enhancer-binding protein homologous protein gene (chop), fused with the GFP reporter and driven by a cytomegalovirus promoter [54] . For example, the Tsai's lab established a transgenic line which could be induced to knock down the expression level of cardiac troponin C at any developmental stage, including embryos, larva or adult fish.	./cache/cord-001921-73esrper.txt	./txt/cord-001921-73esrper.txt