id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-306371-jmaqkovs	Jensen, Ditte Marie Krohn	Spray drying of siRNA-containing PLGA nanoparticles intended for inhalation	2010-02-25		.txt	text/plain	6629	342	53	The present study evaluated parameters of importance for spray drying of siRNA-loaded poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) into nanocomposite microparticles intended for inhalation. The identified optimum conditions were applied for spray drying of siRNA-loaded nanocomposite microparticles, resulting in a product with a low water content (0.78% w/w) and an aerodynamic particle diameter considered suitable for inhalation. In conclusion, the present results show that spray drying is a suitable technique for producing nanocomposite microparticles comprising siRNA-containing PLGA NPs for potential use in inhalation therapy. To overcome the macrophage clearance and still benefit from a particle size suitable for deep lung deposition and sustained release, the present study employs nanocomposite microparticles consisting of co-spray-dried PLGA NPs and a carbohydrate excipient. The optimum formulation found to provide a low water content, a sufficient yield and a small particle size was with mannitol as the carbohydrate excipient, a total dry substance concentration of 30 mg/ mL and a ratio of NPs to excipient of 0.2.	./cache/cord-306371-jmaqkovs.txt	./txt/cord-306371-jmaqkovs.txt