key: cord-299222-vmofcrim authors: Rivas‐Pollmar, María Isabel; Álvarez‐Román, María Teresa; Butta‐Coll, Nora V.; Martín Salces, Mónica; García‐Barcenilla, Sara; Jiménez‐Yuste, Victor title: Thromboprophylaxis in a patient with COVID‐19 and severe hemophilia A on emicizumab prophylaxis date: 2020-06-11 journal: J Thromb Haemost DOI: 10.1111/jth.14954 sha: doc_id: 299222 cord_uid: vmofcrim COVID‐19 can be associated with coagulopathy (CAC, COVID‐19‐associated coagulopathy) with a high prothrombotic risk based on an intense inflammatory response to viral infection leading to immunothrombosis through different procoagulant pathways[1]. Emerging evidence suggests that the use of heparin in these patients could be associated with lower mortality[2]. COVID-19 can be associated with coagulopathy (CAC, COVID-19-associated coagulopathy) with a high prothrombotic risk based on an intense inflammatory response to viral infection leading to immunothrombosis through different procoagulant pathways [1] . Emerging evidence suggests that the use of heparin in these patients could be associated with lower mortality [2] . Emicizumab is a bispecific humanized monoclonal antibody that bridges activated factor IX and factor X, thereby restoring the function of missing FVIIIa in hemophilia A; it has been impressive in reducing bleeding in the treatment of patients with hemophilia A with and without inhibitors [3, 4] . The use of emicizumab has been associated with thrombotic events in patients who also received high cumulative amounts of aPCC [5] . Although this risk is extremely low, there is a lack of evidence on whether CAC increases the thrombotic risk in patients on emicizumab prophylaxis. We were therefore faced, in this situation, with a patient with severe hemophilia A (SHA) on emicizumab prophylaxis with a diagnosis of COVID-19. The patient was a 49-year-old male with SHA without inhibitors. The patient had been on prophylactic treatment with emicizumab at 6 mg/kg once every four weeks since May 2017 when he was included in a clinical trial (HAVEN 4 ClinicalTrials.gov, number NCT03020160). He had HIV infection with antiretroviral treatment (lamivudine and darunavir-cobicistat) with good HIV virologic response and immunologic recovery. He had also been treated with antivirals for hepatitis C virus (HCV). In November 2018 he was diagnosed with non-Hodgkin Diffuse Large Activated-B cell Lymphoma stage IV. He received 6 cycles of R-CHOP achieving a complete remission in May 2019. On April 2020 he attended the Emergency Department with malaise, cough, myalgia, a feverish sensation, anosmia and dysgeusia of four days' duration. He had no dyspnea or chest pain. SARS-COV-2 PCR was positive. The chest angio-TC showed no COVID-19-compatible disease or pulmonary thromboembolism. Because of the mild disease and respiratory stability, the patient was discharged with domiciliary followup by the Hemophilia Unit. He did not receive hydroxychloroquine as he had no radiological evidence of COVID-19 disease and because he was on HIV antiretroviral therapy. This article is protected by copyright. All rights reserved Due to uncertainty about the risk of an increased prothrombotic state in CAC in association with emicizumab prophylaxis, thromboprophylaxis with low molecular weight heparin (LMWH), enoxaparin 40 mg once daily, was started. The patient's clinical course was good without any bleeding or thrombotic complications and he continued on the same regimen of emicizumab prophylaxis; LMWH prophylaxis was stopped after the first negative SARS-CoV-2 PCR on day 26 of onset of symptoms and after 21days of LMWH treatment. The patient consented to the use of his data and has been approved by the local Ethics Board of our Center. Emicizumab prophylaxis is associated with great efficacy in patients with hemophilia without inhibitors [4] . It has a good safety profile, the commonest side effect being injection site reactions in about 15% of patients. However, some very uncommon adverse events, such as thrombotic microangiopathy (TMA) and venous and arterial thrombosis, have been described, especially in patients with inhibitors and in association with bypassing agents [5] . Emicizumab as a bispecific antibody with two binding regions, one recognizing FIX/FIXa and the other recognizing FX/FXa, promotes FIXa-mediated FX activation. Both FVIIIa and emicizumab increase FXa generation, but with emicizumab, the factor that is limiting FXa generation is no longer FVIIIa but the amount of FIXa that is being generated [3] . This is important because tenase activity is limited by its cofactor activity, so FXa generation is controlled by FVIIIa inactivation, which suggests, as proposed by Lenting, that emicizumab has no on/off mechanism [3] . Furthermore, as emicizumab does not need previous activation to work as a cofactor, an increase in FIXa could contribute to thrombin generation [6] . Regarding the location of FXa generation by emicizumab, it has been postulated that this is re-localized to areas of increased phosphatidyl-serine exposure [3] . Finally, there is little data on patients receiving prophylaxis in the context of sepsis or major trauma [6]. Emerging evidence shows that severe COVID-19 can be complicated by CAC [1, 7] . Inflammation is present in severe COVID-19 patients, with a subgroup exhibiting exacerbation in the inflammatory response with a cytokine storm. This inadequate inflammatory reaction is responsible for the progression of CAC with increased D-dimer, associated with a poorer prognosis [8] . SARS-CoV-2 attacks the endothelial cells, expressing high levels of ACE2 [9] . This endothelialopathy leads to microvascular thrombosis and endothelial damage exposing phosphatidyl-serine [1] . Available data on the management of thrombotic risk is limited and based on case series [10, 11] . Recommendations suggest that in cases where an increased thrombotic risk is suspected, pharmacologic This article is protected by copyright. All rights reserved venous thromboembolism (VTE) prophylaxis in all hospitalized COVID-19 patients should be initiated as long as there is no contraindication [12] . The ideal dose and type of heparin remains unclear, pending randomized trials on this subject. Our case had an initial mild presentation of COVID-19 disease but presented an uncertain risk of CAC due to the prophylaxis with emicizumab. Bearing in mind the low risk of thrombosis with emicizumab in SHA without inhibitors, but because some of the mechanisms associated with the risk of thrombosis described above could be risk factors for the development of CAC, and in the absence of literature or guidelines for the management of such patients, our consensus clinical decision was to administer prophylactic doses of LMWH and to monitor laboratory parameters to follow up the disease and cytokine release storm. Low molecular weight heparin (LMWH) inhibits mainly FXa which cleaves FII to FIIa, but like unfractionated heparin it also has indirect inhibition of FIIa itself. Some authors suggested that aPTT could reflect the real clinical effect of LMWH under specific conditions [13] . In our case the aPTT was always below the normal range, which corresponds to emicizumab treatment, so we decided to monitor thromboprophylaxis with anti-FXa levels, as these describe pharmacokinetics rather than pharmacodynamics, but to be sure not to exceed prophylactic levels. The patient had no bleeding complications, but it was unclear when was the ideal time to stop thromboprophylaxis in the absence of guidelines; taking into account that the laboratory parameters had all been stable since the diagnosis, we decided to stop thromboprophylaxis based on negative SARS-CoV-2 PCR. Fortunately, the laboratory findings of our patient did not worsen and the clinical course was also favourable. This mild and favourable course of our patient with SHA on emicizumab prophylaxis could be interpreted in the same way as for around 85% of COVID-19 patients, i.e. neither the hemophilia nor the emicizumab treatment has a significant influence on the course of the disease, or that, however SHA and/or emicizumab and/or HIV status has any protective effect against inflammation and immunothrombosis per se. Otherwise, heparin has an anti-inflammatory effect and its use in a SHA patient on emicizumab prophylaxis could help to prevent a cytokine storm, having protective effect against CAC. We do not know to what extent the protective effect of LMWH counteracts the potential prothrombotic state of COVID-19 and emicizumab. But we proposed the use of prophylactic dose of LMWH in SHA on emicizumab treatment at diagnosis, based on the low rate of bleeding complications associated with its This article is protected by copyright. All rights reserved use, the lack of predictive parameters of CAC development and the potential prothrombotic state of emicizumab treatment in the context of sepsis Induced coagulopathy. COVID-19 and its implications for thrombosis and anticoagulation Anticoagulant treatment is associated with decreased mortality in severe coronavirus disease 2019 patients with coagulopathy Emicizumab, a bispecific antibody recognizing coagulation factors IX and X: how does it actually compare to factor VIII? Blood Emicizumab Prophylaxis in Patients Who Have Hemophilia A without Inhibitors Emicizumab Prophylaxis in Hemophilia A with Inhibitors Accepted Article 176 Thromboembolic risk and anticoagulant therapy in COVID-19 patients: emerging evidence and call for action D-dimer levels on admission to predict in-hospital mortality in patients with Covid-19 SARS-CoV-2 and viral sepsis: observations and hypotheses Incidence of thrombotic complications in critically ill ICU patients with COVID-19 High risk of thrombosis in patients with severe SARS-CoV-2 infection: a multicenter prospective cohort study ISTH interim guidance on recognition and management of coagulopathy in COVID-19 Monitoring low molecular weight heparins at therapeutic levels: dose-responses of, and correlations and differences between aPTT, anti-factor Xa and thrombin generation assays VJY were responsable for the clinical management of the patient, SGB was the nurse who administered the treatment, and NBC was responsible for the laboratory tests. CONFLICT OF INTEREST STATEMENT The autor Victor Jimenez Yuste has received reimbursement for attending symposia/congresses and/or honoraria for speaking and/or honoraria for consulting, and/or founds for research from Takeda