id sid tid token lemma pos cord-303381-xvzhb7ix 1 1 key key NN cord-303381-xvzhb7ix 1 2 : : : cord-303381-xvzhb7ix 2 1 cord-303381-xvzhb7ix cord-303381-xvzhb7ix . cord-303381-xvzhb7ix 3 1 authors author NNS cord-303381-xvzhb7ix 3 2 : : : cord-303381-xvzhb7ix 3 3 TSURUTA TSURUTA NNP cord-303381-xvzhb7ix 3 4 , , , cord-303381-xvzhb7ix 3 5 Yuya Yuya NNP cord-303381-xvzhb7ix 3 6 ; ; : cord-303381-xvzhb7ix 3 7 SHIBUTANI SHIBUTANI NNP cord-303381-xvzhb7ix 3 8 , , , cord-303381-xvzhb7ix 3 9 Shusaku Shusaku NNP cord-303381-xvzhb7ix 3 10 T. T. NNP cord-303381-xvzhb7ix 3 11 ; ; : cord-303381-xvzhb7ix 3 12 WATANABE WATANABE NNP cord-303381-xvzhb7ix 3 13 , , , cord-303381-xvzhb7ix 3 14 Rie Rie NNP cord-303381-xvzhb7ix 3 15 ; ; : cord-303381-xvzhb7ix 3 16 IWATA IWATA NNP cord-303381-xvzhb7ix 3 17 , , , cord-303381-xvzhb7ix 3 18 Hiroyuki Hiroyuki NNP cord-303381-xvzhb7ix 3 19 title title NN cord-303381-xvzhb7ix 3 20 : : : cord-303381-xvzhb7ix 4 1 The the DT cord-303381-xvzhb7ix 4 2 requirement requirement NN cord-303381-xvzhb7ix 4 3 of of IN cord-303381-xvzhb7ix 4 4 environmental environmental JJ cord-303381-xvzhb7ix 4 5 acidification acidification NN cord-303381-xvzhb7ix 4 6 for for IN cord-303381-xvzhb7ix 4 7 Ibaraki Ibaraki NNP cord-303381-xvzhb7ix 4 8 virus virus NN cord-303381-xvzhb7ix 4 9 infection infection NN cord-303381-xvzhb7ix 4 10 to to TO cord-303381-xvzhb7ix 4 11 host host NN cord-303381-xvzhb7ix 4 12 cells cell NNS cord-303381-xvzhb7ix 4 13 date date NN cord-303381-xvzhb7ix 4 14 : : : cord-303381-xvzhb7ix 4 15 2015 2015 CD cord-303381-xvzhb7ix 4 16 - - HYPH cord-303381-xvzhb7ix 4 17 08 08 CD cord-303381-xvzhb7ix 4 18 - - HYPH cord-303381-xvzhb7ix 4 19 28 28 CD cord-303381-xvzhb7ix 4 20 journal journal NN cord-303381-xvzhb7ix 4 21 : : : cord-303381-xvzhb7ix 4 22 J J NNP cord-303381-xvzhb7ix 4 23 Vet Vet NNP cord-303381-xvzhb7ix 4 24 Med Med NNP cord-303381-xvzhb7ix 5 1 Sci Sci NNP cord-303381-xvzhb7ix 5 2 DOI DOI NNP cord-303381-xvzhb7ix 6 1 : : : cord-303381-xvzhb7ix 6 2 10.1292 10.1292 CD cord-303381-xvzhb7ix 6 3 / / SYM cord-303381-xvzhb7ix 6 4 jvms.15 jvms.15 NN cord-303381-xvzhb7ix 6 5 - - HYPH cord-303381-xvzhb7ix 6 6 0222 0222 CD cord-303381-xvzhb7ix 6 7 sha sha NNP cord-303381-xvzhb7ix 6 8 : : : cord-303381-xvzhb7ix 7 1 5d352d8eaadb17dc1ed4cd7501e3533d366c939c 5d352d8eaadb17dc1ed4cd7501e3533d366c939c LS cord-303381-xvzhb7ix 7 2 doc_id doc_id CD cord-303381-xvzhb7ix 7 3 : : : cord-303381-xvzhb7ix 7 4 303381 303381 CD cord-303381-xvzhb7ix 7 5 cord_uid cord_uid NNS cord-303381-xvzhb7ix 7 6 : : : cord-303381-xvzhb7ix 8 1 xvzhb7ix xvzhb7ix NNP cord-303381-xvzhb7ix 9 1 The the DT cord-303381-xvzhb7ix 9 2 effect effect NN cord-303381-xvzhb7ix 9 3 of of IN cord-303381-xvzhb7ix 9 4 environmental environmental JJ cord-303381-xvzhb7ix 9 5 acidification acidification NN cord-303381-xvzhb7ix 9 6 on on IN cord-303381-xvzhb7ix 9 7 Ibaraki Ibaraki NNP cord-303381-xvzhb7ix 9 8 virus virus NN cord-303381-xvzhb7ix 9 9 ( ( -LRB- cord-303381-xvzhb7ix 9 10 IBAV IBAV NNP cord-303381-xvzhb7ix 9 11 ) ) -RRB- cord-303381-xvzhb7ix 9 12 infection infection NN cord-303381-xvzhb7ix 9 13 was be VBD cord-303381-xvzhb7ix 9 14 tested test VBN cord-303381-xvzhb7ix 9 15 using use VBG cord-303381-xvzhb7ix 9 16 endosomal endosomal NNP cord-303381-xvzhb7ix 9 17 inhibitory inhibitory JJ cord-303381-xvzhb7ix 9 18 chemicals chemical NNS cord-303381-xvzhb7ix 9 19 and and CC cord-303381-xvzhb7ix 9 20 low low JJ cord-303381-xvzhb7ix 9 21 pH pH NNP cord-303381-xvzhb7ix 9 22 treatment treatment NN cord-303381-xvzhb7ix 9 23 . . . cord-303381-xvzhb7ix 10 1 Treatment treatment NN cord-303381-xvzhb7ix 10 2 of of IN cord-303381-xvzhb7ix 10 3 target target NN cord-303381-xvzhb7ix 10 4 cells cell NNS cord-303381-xvzhb7ix 10 5 with with IN cord-303381-xvzhb7ix 10 6 endosomal endosomal NNP cord-303381-xvzhb7ix 10 7 inhibitors inhibitor NNS cord-303381-xvzhb7ix 10 8 significantly significantly RB cord-303381-xvzhb7ix 10 9 decreased decrease VBD cord-303381-xvzhb7ix 10 10 the the DT cord-303381-xvzhb7ix 10 11 progeny progeny JJ cord-303381-xvzhb7ix 10 12 virus virus NN cord-303381-xvzhb7ix 10 13 production production NN cord-303381-xvzhb7ix 10 14 . . . cord-303381-xvzhb7ix 11 1 IBAV IBAV NNP cord-303381-xvzhb7ix 11 2 outer outer JJ cord-303381-xvzhb7ix 11 3 capsid capsid NN cord-303381-xvzhb7ix 11 4 proteins protein NNS cord-303381-xvzhb7ix 11 5 , , , cord-303381-xvzhb7ix 11 6 VP5 VP5 NNS cord-303381-xvzhb7ix 11 7 and and CC cord-303381-xvzhb7ix 11 8 VP2 vp2 NN cord-303381-xvzhb7ix 11 9 , , , cord-303381-xvzhb7ix 11 10 were be VBD cord-303381-xvzhb7ix 11 11 removed remove VBN cord-303381-xvzhb7ix 11 12 from from IN cord-303381-xvzhb7ix 11 13 virion virion NN cord-303381-xvzhb7ix 11 14 when when WRB cord-303381-xvzhb7ix 11 15 purified purify VBN cord-303381-xvzhb7ix 11 16 IBAV IBAV NNP cord-303381-xvzhb7ix 11 17 was be VBD cord-303381-xvzhb7ix 11 18 exposed expose VBN cord-303381-xvzhb7ix 11 19 to to IN cord-303381-xvzhb7ix 11 20 low low JJ cord-303381-xvzhb7ix 11 21 pH pH NNP cord-303381-xvzhb7ix 11 22 environment environment NN cord-303381-xvzhb7ix 11 23 . . . cord-303381-xvzhb7ix 12 1 Further further JJ cord-303381-xvzhb7ix 12 2 experiment experiment NN cord-303381-xvzhb7ix 12 3 showed show VBD cord-303381-xvzhb7ix 12 4 that that IN cord-303381-xvzhb7ix 12 5 the the DT cord-303381-xvzhb7ix 12 6 exposure exposure NN cord-303381-xvzhb7ix 12 7 to to IN cord-303381-xvzhb7ix 12 8 low low JJ cord-303381-xvzhb7ix 12 9 pH pH NNP cord-303381-xvzhb7ix 12 10 buffer buffer NN cord-303381-xvzhb7ix 12 11 facilitated facilitate VBD cord-303381-xvzhb7ix 12 12 IBAV IBAV NNP cord-303381-xvzhb7ix 12 13 infection infection NN cord-303381-xvzhb7ix 12 14 when when WRB cord-303381-xvzhb7ix 12 15 the the DT cord-303381-xvzhb7ix 12 16 cellular cellular JJ cord-303381-xvzhb7ix 12 17 endosomal endosomal NNP cord-303381-xvzhb7ix 12 18 pathway pathway NN cord-303381-xvzhb7ix 12 19 was be VBD cord-303381-xvzhb7ix 12 20 impaired impair VBN cord-303381-xvzhb7ix 12 21 by by IN cord-303381-xvzhb7ix 12 22 bafilomycin bafilomycin NNP cord-303381-xvzhb7ix 12 23 A1 A1 NNP cord-303381-xvzhb7ix 12 24 . . . cord-303381-xvzhb7ix 13 1 Results result NNS cord-303381-xvzhb7ix 13 2 obtained obtain VBN cord-303381-xvzhb7ix 13 3 in in IN cord-303381-xvzhb7ix 13 4 this this DT cord-303381-xvzhb7ix 13 5 study study NN cord-303381-xvzhb7ix 13 6 suggest suggest VBP cord-303381-xvzhb7ix 13 7 that that IN cord-303381-xvzhb7ix 13 8 acidic acidic JJ cord-303381-xvzhb7ix 13 9 environment environment NN cord-303381-xvzhb7ix 13 10 is be VBZ cord-303381-xvzhb7ix 13 11 essential essential JJ cord-303381-xvzhb7ix 13 12 to to TO cord-303381-xvzhb7ix 13 13 initiate initiate VB cord-303381-xvzhb7ix 13 14 IBAV IBAV NNP cord-303381-xvzhb7ix 13 15 infection infection NN cord-303381-xvzhb7ix 13 16 . . . cord-303381-xvzhb7ix 14 1 Ibaraki Ibaraki NNP cord-303381-xvzhb7ix 14 2 virus virus NN cord-303381-xvzhb7ix 14 3 ( ( -LRB- cord-303381-xvzhb7ix 14 4 IBAV IBAV NNP cord-303381-xvzhb7ix 14 5 ) ) -RRB- cord-303381-xvzhb7ix 14 6 is be VBZ cord-303381-xvzhb7ix 14 7 one one CD cord-303381-xvzhb7ix 14 8 of of IN cord-303381-xvzhb7ix 14 9 the the DT cord-303381-xvzhb7ix 14 10 strains strain NNS cord-303381-xvzhb7ix 14 11 of of IN cord-303381-xvzhb7ix 14 12 epizootic epizootic JJ cord-303381-xvzhb7ix 14 13 hemorrhagic hemorrhagic JJ cord-303381-xvzhb7ix 14 14 disease disease NN cord-303381-xvzhb7ix 14 15 virus virus NN cord-303381-xvzhb7ix 14 16 ( ( -LRB- cord-303381-xvzhb7ix 14 17 EHDV EHDV NNP cord-303381-xvzhb7ix 14 18 ) ) -RRB- cord-303381-xvzhb7ix 14 19 serotype serotype NN cord-303381-xvzhb7ix 14 20 2 2 CD cord-303381-xvzhb7ix 14 21 , , , cord-303381-xvzhb7ix 14 22 a a DT cord-303381-xvzhb7ix 14 23 member member NN cord-303381-xvzhb7ix 14 24 of of IN cord-303381-xvzhb7ix 14 25 genus genus NN cord-303381-xvzhb7ix 14 26 Orbivirus Orbivirus NNP cord-303381-xvzhb7ix 14 27 in in IN cord-303381-xvzhb7ix 14 28 Reoviridae Reoviridae NNP cord-303381-xvzhb7ix 14 29 family family NN cord-303381-xvzhb7ix 14 30 [ [ -LRB- cord-303381-xvzhb7ix 14 31 11 11 CD cord-303381-xvzhb7ix 14 32 , , , cord-303381-xvzhb7ix 14 33 19 19 CD cord-303381-xvzhb7ix 14 34 ] ] -RRB- cord-303381-xvzhb7ix 14 35 . . . cord-303381-xvzhb7ix 15 1 It -PRON- PRP cord-303381-xvzhb7ix 15 2 is be VBZ cord-303381-xvzhb7ix 15 3 known know VBN cord-303381-xvzhb7ix 15 4 as as IN cord-303381-xvzhb7ix 15 5 a a DT cord-303381-xvzhb7ix 15 6 causative causative JJ cord-303381-xvzhb7ix 15 7 agent agent NN cord-303381-xvzhb7ix 15 8 of of IN cord-303381-xvzhb7ix 15 9 Ibaraki Ibaraki NNP cord-303381-xvzhb7ix 15 10 disease disease NN cord-303381-xvzhb7ix 15 11 characterized characterize VBN cord-303381-xvzhb7ix 15 12 by by IN cord-303381-xvzhb7ix 15 13 the the DT cord-303381-xvzhb7ix 15 14 paralysis paralysis NN cord-303381-xvzhb7ix 15 15 of of IN cord-303381-xvzhb7ix 15 16 swallowing swallow VBG cord-303381-xvzhb7ix 15 17 muscles muscle NNS cord-303381-xvzhb7ix 15 18 . . . cord-303381-xvzhb7ix 16 1 Although although IN cord-303381-xvzhb7ix 16 2 the the DT cord-303381-xvzhb7ix 16 3 effective effective JJ cord-303381-xvzhb7ix 16 4 vaccine vaccine NN cord-303381-xvzhb7ix 16 5 is be VBZ cord-303381-xvzhb7ix 16 6 widely widely RB cord-303381-xvzhb7ix 16 7 used use VBN cord-303381-xvzhb7ix 16 8 , , , cord-303381-xvzhb7ix 16 9 the the DT cord-303381-xvzhb7ix 16 10 recent recent JJ cord-303381-xvzhb7ix 16 11 reemergence reemergence NN cord-303381-xvzhb7ix 16 12 of of IN cord-303381-xvzhb7ix 16 13 Ibaraki Ibaraki NNP cord-303381-xvzhb7ix 16 14 disease disease NN cord-303381-xvzhb7ix 16 15 in in IN cord-303381-xvzhb7ix 16 16 combination combination NN cord-303381-xvzhb7ix 16 17 with with IN cord-303381-xvzhb7ix 16 18 the the DT cord-303381-xvzhb7ix 16 19 co co NN cord-303381-xvzhb7ix 16 20 - - NN cord-303381-xvzhb7ix 16 21 circulation circulation NN cord-303381-xvzhb7ix 16 22 with with IN cord-303381-xvzhb7ix 16 23 other other JJ cord-303381-xvzhb7ix 16 24 EHDV EHDV NNP cord-303381-xvzhb7ix 16 25 raised raise VBD cord-303381-xvzhb7ix 16 26 the the DT cord-303381-xvzhb7ix 16 27 alert alert JJ cord-303381-xvzhb7ix 16 28 level level NN cord-303381-xvzhb7ix 16 29 for for IN cord-303381-xvzhb7ix 16 30 the the DT cord-303381-xvzhb7ix 16 31 epidemic epidemic NN cord-303381-xvzhb7ix 16 32 of of IN cord-303381-xvzhb7ix 16 33 Ibaraki Ibaraki NNP cord-303381-xvzhb7ix 16 34 disease disease NN cord-303381-xvzhb7ix 16 35 [ [ -LRB- cord-303381-xvzhb7ix 16 36 13 13 CD cord-303381-xvzhb7ix 16 37 ] ] -RRB- cord-303381-xvzhb7ix 16 38 . . . cord-303381-xvzhb7ix 17 1 In in IN cord-303381-xvzhb7ix 17 2 addition addition NN cord-303381-xvzhb7ix 17 3 , , , cord-303381-xvzhb7ix 17 4 orbivirus orbivirus NN cord-303381-xvzhb7ix 17 5 family family NN cord-303381-xvzhb7ix 17 6 contains contain VBZ cord-303381-xvzhb7ix 17 7 many many JJ cord-303381-xvzhb7ix 17 8 viruses virus NNS cord-303381-xvzhb7ix 17 9 causing cause VBG cord-303381-xvzhb7ix 17 10 severe severe JJ cord-303381-xvzhb7ix 17 11 loss loss NN cord-303381-xvzhb7ix 17 12 in in IN cord-303381-xvzhb7ix 17 13 the the DT cord-303381-xvzhb7ix 17 14 livestock livestock NN cord-303381-xvzhb7ix 17 15 industry industry NN cord-303381-xvzhb7ix 17 16 . . . cord-303381-xvzhb7ix 18 1 Therefore therefore RB cord-303381-xvzhb7ix 18 2 , , , cord-303381-xvzhb7ix 18 3 it -PRON- PRP cord-303381-xvzhb7ix 18 4 is be VBZ cord-303381-xvzhb7ix 18 5 important important JJ cord-303381-xvzhb7ix 18 6 to to TO cord-303381-xvzhb7ix 18 7 understand understand VB cord-303381-xvzhb7ix 18 8 the the DT cord-303381-xvzhb7ix 18 9 detailed detailed JJ cord-303381-xvzhb7ix 18 10 molecular molecular JJ cord-303381-xvzhb7ix 18 11 mechanism mechanism NN cord-303381-xvzhb7ix 18 12 of of IN cord-303381-xvzhb7ix 18 13 their -PRON- PRP$ cord-303381-xvzhb7ix 18 14 infection infection NN cord-303381-xvzhb7ix 18 15 . . . cord-303381-xvzhb7ix 19 1 Virus virus NN cord-303381-xvzhb7ix 19 2 infection infection NN cord-303381-xvzhb7ix 19 3 is be VBZ cord-303381-xvzhb7ix 19 4 initiated initiate VBN cord-303381-xvzhb7ix 19 5 by by IN cord-303381-xvzhb7ix 19 6 the the DT cord-303381-xvzhb7ix 19 7 release release NN cord-303381-xvzhb7ix 19 8 of of IN cord-303381-xvzhb7ix 19 9 viral viral JJ cord-303381-xvzhb7ix 19 10 genome genome NN cord-303381-xvzhb7ix 19 11 to to IN cord-303381-xvzhb7ix 19 12 the the DT cord-303381-xvzhb7ix 19 13 intracellular intracellular JJ cord-303381-xvzhb7ix 19 14 compartment compartment NN cord-303381-xvzhb7ix 19 15 of of IN cord-303381-xvzhb7ix 19 16 target target NN cord-303381-xvzhb7ix 19 17 cells cell NNS cord-303381-xvzhb7ix 19 18 . . . cord-303381-xvzhb7ix 20 1 Several several JJ cord-303381-xvzhb7ix 20 2 mechanisms mechanism NNS cord-303381-xvzhb7ix 20 3 are be VBP cord-303381-xvzhb7ix 20 4 known know VBN cord-303381-xvzhb7ix 20 5 for for IN cord-303381-xvzhb7ix 20 6 the the DT cord-303381-xvzhb7ix 20 7 genome genome NN cord-303381-xvzhb7ix 20 8 release release NN cord-303381-xvzhb7ix 20 9 to to TO cord-303381-xvzhb7ix 20 10 cytoplasm cytoplasm VB cord-303381-xvzhb7ix 20 11 , , , cord-303381-xvzhb7ix 20 12 and and CC cord-303381-xvzhb7ix 20 13 one one CD cord-303381-xvzhb7ix 20 14 of of IN cord-303381-xvzhb7ix 20 15 the the DT cord-303381-xvzhb7ix 20 16 most most RBS cord-303381-xvzhb7ix 20 17 well well RB cord-303381-xvzhb7ix 20 18 known know VBN cord-303381-xvzhb7ix 20 19 mechanisms mechanism NNS cord-303381-xvzhb7ix 20 20 is be VBZ cord-303381-xvzhb7ix 20 21 the the DT cord-303381-xvzhb7ix 20 22 direct direct JJ cord-303381-xvzhb7ix 20 23 fusion fusion NN cord-303381-xvzhb7ix 20 24 mechanism mechanism NN cord-303381-xvzhb7ix 20 25 utilized utilize VBN cord-303381-xvzhb7ix 20 26 by by IN cord-303381-xvzhb7ix 20 27 several several JJ cord-303381-xvzhb7ix 20 28 enveloped envelop VBN cord-303381-xvzhb7ix 20 29 viruses virus NNS cord-303381-xvzhb7ix 20 30 , , , cord-303381-xvzhb7ix 20 31 such such JJ cord-303381-xvzhb7ix 20 32 as as IN cord-303381-xvzhb7ix 20 33 human human JJ cord-303381-xvzhb7ix 20 34 immunodeficiency immunodeficiency NN cord-303381-xvzhb7ix 20 35 virus-1 virus-1 NN cord-303381-xvzhb7ix 20 36 , , , cord-303381-xvzhb7ix 20 37 mouse mouse NN cord-303381-xvzhb7ix 20 38 hepatitis hepatitis NN cord-303381-xvzhb7ix 20 39 virus virus NN cord-303381-xvzhb7ix 20 40 and and CC cord-303381-xvzhb7ix 20 41 Paramyxoviruses Paramyxoviruses NNP cord-303381-xvzhb7ix 20 42 [ [ -LRB- cord-303381-xvzhb7ix 20 43 5 5 CD cord-303381-xvzhb7ix 20 44 , , , cord-303381-xvzhb7ix 20 45 6 6 CD cord-303381-xvzhb7ix 20 46 ] ] -RRB- cord-303381-xvzhb7ix 20 47 . . . cord-303381-xvzhb7ix 21 1 Enveloped envelop VBN cord-303381-xvzhb7ix 21 2 viruses virus NNS cord-303381-xvzhb7ix 21 3 are be VBP cord-303381-xvzhb7ix 21 4 also also RB cord-303381-xvzhb7ix 21 5 known know VBN cord-303381-xvzhb7ix 21 6 to to TO cord-303381-xvzhb7ix 21 7 use use VB cord-303381-xvzhb7ix 21 8 host host NN cord-303381-xvzhb7ix 21 9 endosomal endosomal NNP cord-303381-xvzhb7ix 21 10 pathway pathway NN cord-303381-xvzhb7ix 21 11 to to TO cord-303381-xvzhb7ix 21 12 facilitate facilitate VB cord-303381-xvzhb7ix 21 13 their -PRON- PRP$ cord-303381-xvzhb7ix 21 14 infection infection NN cord-303381-xvzhb7ix 21 15 [ [ -LRB- cord-303381-xvzhb7ix 21 16 12 12 CD cord-303381-xvzhb7ix 21 17 ] ] -RRB- cord-303381-xvzhb7ix 21 18 . . . cord-303381-xvzhb7ix 22 1 Reovirus Reovirus NNP cord-303381-xvzhb7ix 22 2 family family NN cord-303381-xvzhb7ix 22 3 does do VBZ cord-303381-xvzhb7ix 22 4 not not RB cord-303381-xvzhb7ix 22 5 have have VB cord-303381-xvzhb7ix 22 6 envelope envelope NN cord-303381-xvzhb7ix 22 7 , , , cord-303381-xvzhb7ix 22 8 and and CC cord-303381-xvzhb7ix 22 9 its -PRON- PRP$ cord-303381-xvzhb7ix 22 10 infection infection NN cord-303381-xvzhb7ix 22 11 to to TO cord-303381-xvzhb7ix 22 12 host host NN cord-303381-xvzhb7ix 22 13 cells cell NNS cord-303381-xvzhb7ix 22 14 is be VBZ cord-303381-xvzhb7ix 22 15 believed believe VBN cord-303381-xvzhb7ix 22 16 to to TO cord-303381-xvzhb7ix 22 17 occur occur VB cord-303381-xvzhb7ix 22 18 via via IN cord-303381-xvzhb7ix 22 19 host host NN cord-303381-xvzhb7ix 22 20 endosomal endosomal NNP cord-303381-xvzhb7ix 22 21 pathway pathway NNP cord-303381-xvzhb7ix 22 22 [ [ -LRB- cord-303381-xvzhb7ix 22 23 2 2 CD cord-303381-xvzhb7ix 22 24 , , , cord-303381-xvzhb7ix 22 25 12 12 CD cord-303381-xvzhb7ix 22 26 ] ] -RRB- cord-303381-xvzhb7ix 22 27 . . . cord-303381-xvzhb7ix 23 1 Once once RB cord-303381-xvzhb7ix 23 2 attached attach VBN cord-303381-xvzhb7ix 23 3 to to IN cord-303381-xvzhb7ix 23 4 the the DT cord-303381-xvzhb7ix 23 5 cell cell NN cord-303381-xvzhb7ix 23 6 surface surface NN cord-303381-xvzhb7ix 23 7 , , , cord-303381-xvzhb7ix 23 8 the the DT cord-303381-xvzhb7ix 23 9 host host NN cord-303381-xvzhb7ix 23 10 endosomal endosomal NNP cord-303381-xvzhb7ix 23 11 pathway pathway NN cord-303381-xvzhb7ix 23 12 incorporates incorporate VBZ cord-303381-xvzhb7ix 23 13 particles particle NNS cord-303381-xvzhb7ix 23 14 . . . cord-303381-xvzhb7ix 24 1 Along along IN cord-303381-xvzhb7ix 24 2 with with IN cord-303381-xvzhb7ix 24 3 the the DT cord-303381-xvzhb7ix 24 4 endosome endosome NN cord-303381-xvzhb7ix 24 5 maturation maturation NN cord-303381-xvzhb7ix 24 6 , , , cord-303381-xvzhb7ix 24 7 the the DT cord-303381-xvzhb7ix 24 8 dissociation dissociation NN cord-303381-xvzhb7ix 24 9 of of IN cord-303381-xvzhb7ix 24 10 outermost outermost JJ cord-303381-xvzhb7ix 24 11 capsid capsid NN cord-303381-xvzhb7ix 24 12 protein protein NN cord-303381-xvzhb7ix 24 13 and and CC cord-303381-xvzhb7ix 24 14 proteolytic proteolytic JJ cord-303381-xvzhb7ix 24 15 cleavage cleavage NN cord-303381-xvzhb7ix 24 16 on on IN cord-303381-xvzhb7ix 24 17 viral viral JJ cord-303381-xvzhb7ix 24 18 protein protein NN cord-303381-xvzhb7ix 24 19 will will MD cord-303381-xvzhb7ix 24 20 occur occur VB cord-303381-xvzhb7ix 24 21 [ [ -LRB- cord-303381-xvzhb7ix 24 22 3 3 CD cord-303381-xvzhb7ix 24 23 , , , cord-303381-xvzhb7ix 24 24 4 4 CD cord-303381-xvzhb7ix 24 25 ] ] -RRB- cord-303381-xvzhb7ix 24 26 . . . cord-303381-xvzhb7ix 25 1 Especially especially RB cord-303381-xvzhb7ix 25 2 , , , cord-303381-xvzhb7ix 25 3 the the DT cord-303381-xvzhb7ix 25 4 proteolytic proteolytic JJ cord-303381-xvzhb7ix 25 5 cleavage cleavage NN cord-303381-xvzhb7ix 25 6 by by IN cord-303381-xvzhb7ix 25 7 endosomal endosomal NNP cord-303381-xvzhb7ix 25 8 proteases protease NNS cord-303381-xvzhb7ix 25 9 , , , cord-303381-xvzhb7ix 25 10 cathepsin cathepsin NN cord-303381-xvzhb7ix 25 11 B B NNP cord-303381-xvzhb7ix 25 12 and and CC cord-303381-xvzhb7ix 25 13 L L NNP cord-303381-xvzhb7ix 25 14 , , , cord-303381-xvzhb7ix 25 15 is be VBZ cord-303381-xvzhb7ix 25 16 an an DT cord-303381-xvzhb7ix 25 17 essential essential JJ cord-303381-xvzhb7ix 25 18 process process NN cord-303381-xvzhb7ix 25 19 for for IN cord-303381-xvzhb7ix 25 20 infection infection NN cord-303381-xvzhb7ix 25 21 [ [ -LRB- cord-303381-xvzhb7ix 25 22 1 1 CD cord-303381-xvzhb7ix 25 23 ] ] -RRB- cord-303381-xvzhb7ix 25 24 . . . cord-303381-xvzhb7ix 26 1 In in IN cord-303381-xvzhb7ix 26 2 other other JJ cord-303381-xvzhb7ix 26 3 words word NNS cord-303381-xvzhb7ix 26 4 , , , cord-303381-xvzhb7ix 26 5 the the DT cord-303381-xvzhb7ix 26 6 expression expression NN cord-303381-xvzhb7ix 26 7 level level NN cord-303381-xvzhb7ix 26 8 of of IN cord-303381-xvzhb7ix 26 9 those those DT cord-303381-xvzhb7ix 26 10 proteases protease NNS cord-303381-xvzhb7ix 26 11 affects affect VBZ cord-303381-xvzhb7ix 26 12 the the DT cord-303381-xvzhb7ix 26 13 susceptibility susceptibility NN cord-303381-xvzhb7ix 26 14 of of IN cord-303381-xvzhb7ix 26 15 the the DT cord-303381-xvzhb7ix 26 16 host host NN cord-303381-xvzhb7ix 26 17 cells cell NNS cord-303381-xvzhb7ix 26 18 to to IN cord-303381-xvzhb7ix 26 19 virus virus NN cord-303381-xvzhb7ix 26 20 infection infection NN cord-303381-xvzhb7ix 26 21 . . . cord-303381-xvzhb7ix 27 1 In in IN cord-303381-xvzhb7ix 27 2 addition addition NN cord-303381-xvzhb7ix 27 3 to to IN cord-303381-xvzhb7ix 27 4 endosomal endosomal NNP cord-303381-xvzhb7ix 27 5 proteases protease NNS cord-303381-xvzhb7ix 27 6 , , , cord-303381-xvzhb7ix 27 7 extracellular extracellular JJ cord-303381-xvzhb7ix 27 8 proteases protease NNS cord-303381-xvzhb7ix 27 9 also also RB cord-303381-xvzhb7ix 27 10 play play VBP cord-303381-xvzhb7ix 27 11 an an DT cord-303381-xvzhb7ix 27 12 important important JJ cord-303381-xvzhb7ix 27 13 role role NN cord-303381-xvzhb7ix 27 14 to to TO cord-303381-xvzhb7ix 27 15 promote promote VB cord-303381-xvzhb7ix 27 16 certain certain JJ cord-303381-xvzhb7ix 27 17 reovirus reovirus JJ cord-303381-xvzhb7ix 27 18 infection infection NN cord-303381-xvzhb7ix 27 19 in in IN cord-303381-xvzhb7ix 27 20 vivo vivo NNP cord-303381-xvzhb7ix 27 21 . . . cord-303381-xvzhb7ix 28 1 It -PRON- PRP cord-303381-xvzhb7ix 28 2 was be VBD cord-303381-xvzhb7ix 28 3 shown show VBN cord-303381-xvzhb7ix 28 4 that that IN cord-303381-xvzhb7ix 28 5 tissue tissue NN cord-303381-xvzhb7ix 28 6 specific specific JJ cord-303381-xvzhb7ix 28 7 proteases protease NNS cord-303381-xvzhb7ix 28 8 contribute contribute VBP cord-303381-xvzhb7ix 28 9 for for IN cord-303381-xvzhb7ix 28 10 the the DT cord-303381-xvzhb7ix 28 11 definition definition NN cord-303381-xvzhb7ix 28 12 of of IN cord-303381-xvzhb7ix 28 13 target target NN cord-303381-xvzhb7ix 28 14 organ organ NN cord-303381-xvzhb7ix 28 15 of of IN cord-303381-xvzhb7ix 28 16 rotavirus rotavirus JJ cord-303381-xvzhb7ix 28 17 infection infection NN cord-303381-xvzhb7ix 28 18 [ [ -LRB- cord-303381-xvzhb7ix 28 19 10 10 CD cord-303381-xvzhb7ix 28 20 ] ] -RRB- cord-303381-xvzhb7ix 28 21 . . . cord-303381-xvzhb7ix 29 1 On on IN cord-303381-xvzhb7ix 29 2 the the DT cord-303381-xvzhb7ix 29 3 other other JJ cord-303381-xvzhb7ix 29 4 hand hand NN cord-303381-xvzhb7ix 29 5 , , , cord-303381-xvzhb7ix 29 6 the the DT cord-303381-xvzhb7ix 29 7 critical critical JJ cord-303381-xvzhb7ix 29 8 condition condition NN cord-303381-xvzhb7ix 29 9 to to TO cord-303381-xvzhb7ix 29 10 activate activate VB cord-303381-xvzhb7ix 29 11 orbivirus orbivirus NN cord-303381-xvzhb7ix 29 12 infection infection NN cord-303381-xvzhb7ix 29 13 remains remain VBZ cord-303381-xvzhb7ix 29 14 unidentified unidentified JJ cord-303381-xvzhb7ix 29 15 . . . cord-303381-xvzhb7ix 30 1 Although although IN cord-303381-xvzhb7ix 30 2 it -PRON- PRP cord-303381-xvzhb7ix 30 3 has have VBZ cord-303381-xvzhb7ix 30 4 been be VBN cord-303381-xvzhb7ix 30 5 suggested suggest VBN cord-303381-xvzhb7ix 30 6 that that IN cord-303381-xvzhb7ix 30 7 orbivirus orbivirus NN cord-303381-xvzhb7ix 30 8 also also RB cord-303381-xvzhb7ix 30 9 utilizes utilize VBZ cord-303381-xvzhb7ix 30 10 host host NN cord-303381-xvzhb7ix 30 11 endosomal endosomal NNP cord-303381-xvzhb7ix 30 12 pathway pathway NN cord-303381-xvzhb7ix 30 13 , , , cord-303381-xvzhb7ix 30 14 no no DT cord-303381-xvzhb7ix 30 15 involvement involvement NN cord-303381-xvzhb7ix 30 16 of of IN cord-303381-xvzhb7ix 30 17 protease protease NN cord-303381-xvzhb7ix 30 18 was be VBD cord-303381-xvzhb7ix 30 19 reported report VBN cord-303381-xvzhb7ix 30 20 [ [ -LRB- cord-303381-xvzhb7ix 30 21 8 8 CD cord-303381-xvzhb7ix 30 22 ] ] -RRB- cord-303381-xvzhb7ix 30 23 . . . cord-303381-xvzhb7ix 31 1 From from IN cord-303381-xvzhb7ix 31 2 studies study NNS cord-303381-xvzhb7ix 31 3 with with IN cord-303381-xvzhb7ix 31 4 enveloped envelop VBN cord-303381-xvzhb7ix 31 5 viruses virus NNS cord-303381-xvzhb7ix 31 6 , , , cord-303381-xvzhb7ix 31 7 it -PRON- PRP cord-303381-xvzhb7ix 31 8 was be VBD cord-303381-xvzhb7ix 31 9 shown show VBN cord-303381-xvzhb7ix 31 10 that that IN cord-303381-xvzhb7ix 31 11 viruses virus NNS cord-303381-xvzhb7ix 31 12 utilize utilize VBP cord-303381-xvzhb7ix 31 13 endosomal endosomal NNP cord-303381-xvzhb7ix 31 14 pathway pathway NN cord-303381-xvzhb7ix 31 15 in in IN cord-303381-xvzhb7ix 31 16 two two CD cord-303381-xvzhb7ix 31 17 different different JJ cord-303381-xvzhb7ix 31 18 ways way NNS cord-303381-xvzhb7ix 31 19 . . . cord-303381-xvzhb7ix 32 1 Some some DT cord-303381-xvzhb7ix 32 2 viruses virus NNS cord-303381-xvzhb7ix 32 3 , , , cord-303381-xvzhb7ix 32 4 such such JJ cord-303381-xvzhb7ix 32 5 as as IN cord-303381-xvzhb7ix 32 6 influenza influenza NN cord-303381-xvzhb7ix 32 7 virus virus NN cord-303381-xvzhb7ix 32 8 , , , cord-303381-xvzhb7ix 32 9 use use VB cord-303381-xvzhb7ix 32 10 low low JJ cord-303381-xvzhb7ix 32 11 pH pH NNP cord-303381-xvzhb7ix 32 12 environment environment NN cord-303381-xvzhb7ix 32 13 itself -PRON- PRP cord-303381-xvzhb7ix 32 14 , , , cord-303381-xvzhb7ix 32 15 and and CC cord-303381-xvzhb7ix 32 16 other other JJ cord-303381-xvzhb7ix 32 17 viruses virus NNS cord-303381-xvzhb7ix 32 18 , , , cord-303381-xvzhb7ix 32 19 such such JJ cord-303381-xvzhb7ix 32 20 as as IN cord-303381-xvzhb7ix 32 21 severe severe JJ cord-303381-xvzhb7ix 32 22 acute acute JJ cord-303381-xvzhb7ix 32 23 respiratory respiratory JJ cord-303381-xvzhb7ix 32 24 syndrome syndrome NN cord-303381-xvzhb7ix 32 25 virus virus NN cord-303381-xvzhb7ix 32 26 , , , cord-303381-xvzhb7ix 32 27 use use VB cord-303381-xvzhb7ix 32 28 endosomal endosomal NNP cord-303381-xvzhb7ix 32 29 proteases protease NNS cord-303381-xvzhb7ix 32 30 rather rather RB cord-303381-xvzhb7ix 32 31 than than IN cord-303381-xvzhb7ix 32 32 low low JJ cord-303381-xvzhb7ix 32 33 pH pH NNP cord-303381-xvzhb7ix 32 34 environment environment NN cord-303381-xvzhb7ix 32 35 itself -PRON- PRP cord-303381-xvzhb7ix 32 36 [ [ -LRB- cord-303381-xvzhb7ix 32 37 12 12 CD cord-303381-xvzhb7ix 32 38 , , , cord-303381-xvzhb7ix 32 39 18 18 CD cord-303381-xvzhb7ix 32 40 ] ] -RRB- cord-303381-xvzhb7ix 32 41 . . . cord-303381-xvzhb7ix 33 1 To to TO cord-303381-xvzhb7ix 33 2 clarify clarify VB cord-303381-xvzhb7ix 33 3 which which WDT cord-303381-xvzhb7ix 33 4 mechanism mechanism NN cord-303381-xvzhb7ix 33 5 was be VBD cord-303381-xvzhb7ix 33 6 used use VBN cord-303381-xvzhb7ix 33 7 by by IN cord-303381-xvzhb7ix 33 8 orbivirus orbivirus NNP cord-303381-xvzhb7ix 33 9 , , , cord-303381-xvzhb7ix 33 10 we -PRON- PRP cord-303381-xvzhb7ix 33 11 tested test VBD cord-303381-xvzhb7ix 33 12 the the DT cord-303381-xvzhb7ix 33 13 effect effect NN cord-303381-xvzhb7ix 33 14 of of IN cord-303381-xvzhb7ix 33 15 environmental environmental JJ cord-303381-xvzhb7ix 33 16 pH ph NN cord-303381-xvzhb7ix 33 17 for for IN cord-303381-xvzhb7ix 33 18 orbivirus orbivirus NN cord-303381-xvzhb7ix 33 19 infectivity infectivity NN cord-303381-xvzhb7ix 33 20 using use VBG cord-303381-xvzhb7ix 33 21 IBAV IBAV NNP cord-303381-xvzhb7ix 33 22 . . . cord-303381-xvzhb7ix 34 1 The the DT cord-303381-xvzhb7ix 34 2 utilization utilization NN cord-303381-xvzhb7ix 34 3 of of IN cord-303381-xvzhb7ix 34 4 endosomal endosomal NNP cord-303381-xvzhb7ix 34 5 pathway pathway NN cord-303381-xvzhb7ix 34 6 by by IN cord-303381-xvzhb7ix 34 7 IBAV IBAV NNP cord-303381-xvzhb7ix 34 8 for for IN cord-303381-xvzhb7ix 34 9 infection infection NN cord-303381-xvzhb7ix 34 10 was be VBD cord-303381-xvzhb7ix 34 11 confirmed confirm VBN cord-303381-xvzhb7ix 34 12 using use VBG cord-303381-xvzhb7ix 34 13 endosome endosome NN cord-303381-xvzhb7ix 34 14 inhibitors inhibitor NNS cord-303381-xvzhb7ix 34 15 . . . cord-303381-xvzhb7ix 35 1 HmLu-1 HmLu-1 NNP cord-303381-xvzhb7ix 35 2 ( ( -LRB- cord-303381-xvzhb7ix 35 3 hamster hamster NN cord-303381-xvzhb7ix 35 4 lung lung NN cord-303381-xvzhb7ix 35 5 ) ) -RRB- cord-303381-xvzhb7ix 35 6 cells cell NNS cord-303381-xvzhb7ix 35 7 were be VBD cord-303381-xvzhb7ix 35 8 infected infect VBN cord-303381-xvzhb7ix 35 9 with with IN cord-303381-xvzhb7ix 35 10 IBAV IBAV NNP cord-303381-xvzhb7ix 35 11 in in IN cord-303381-xvzhb7ix 35 12 the the DT cord-303381-xvzhb7ix 35 13 presence presence NN cord-303381-xvzhb7ix 35 14 of of IN cord-303381-xvzhb7ix 35 15 three three CD cord-303381-xvzhb7ix 35 16 different different JJ cord-303381-xvzhb7ix 35 17 endosome endosome NN cord-303381-xvzhb7ix 35 18 inhibitors inhibitor NNS cord-303381-xvzhb7ix 35 19 , , , cord-303381-xvzhb7ix 35 20 bafilomycin bafilomycin VBZ cord-303381-xvzhb7ix 35 21 A1 A1 NNP cord-303381-xvzhb7ix 35 22 ( ( -LRB- cord-303381-xvzhb7ix 35 23 Baf Baf NNP cord-303381-xvzhb7ix 35 24 A1 A1 NNP cord-303381-xvzhb7ix 35 25 , , , cord-303381-xvzhb7ix 35 26 Sigma Sigma NNP cord-303381-xvzhb7ix 35 27 , , , cord-303381-xvzhb7ix 35 28 St. St. NNP cord-303381-xvzhb7ix 35 29 Louis Louis NNP cord-303381-xvzhb7ix 35 30 , , , cord-303381-xvzhb7ix 35 31 MO MO NNP cord-303381-xvzhb7ix 35 32 , , , cord-303381-xvzhb7ix 35 33 U.S.A. U.S.A. NNP cord-303381-xvzhb7ix 35 34 ) ) -RRB- cord-303381-xvzhb7ix 35 35 , , , cord-303381-xvzhb7ix 35 36 chlorpromazine chlorpromazine NN cord-303381-xvzhb7ix 35 37 ( ( -LRB- cord-303381-xvzhb7ix 35 38 CPZ CPZ NNP cord-303381-xvzhb7ix 35 39 , , , cord-303381-xvzhb7ix 35 40 Abcam Abcam NNP cord-303381-xvzhb7ix 35 41 , , , cord-303381-xvzhb7ix 35 42 Cambridge Cambridge NNP cord-303381-xvzhb7ix 35 43 , , , cord-303381-xvzhb7ix 35 44 U.K. U.K. NNP cord-303381-xvzhb7ix 35 45 ) ) -RRB- cord-303381-xvzhb7ix 35 46 and and CC cord-303381-xvzhb7ix 35 47 dynasore dynasore JJ cord-303381-xvzhb7ix 35 48 ( ( -LRB- cord-303381-xvzhb7ix 35 49 Wako Wako NNP cord-303381-xvzhb7ix 35 50 , , , cord-303381-xvzhb7ix 35 51 Osaka Osaka NNP cord-303381-xvzhb7ix 35 52 , , , cord-303381-xvzhb7ix 35 53 Japan Japan NNP cord-303381-xvzhb7ix 35 54 ) ) -RRB- cord-303381-xvzhb7ix 35 55 . . . cord-303381-xvzhb7ix 36 1 Those those DT cord-303381-xvzhb7ix 36 2 drugs drug NNS cord-303381-xvzhb7ix 36 3 inhibit inhibit VBP cord-303381-xvzhb7ix 36 4 clathrin clathrin NN cord-303381-xvzhb7ix 36 5 dependent dependent JJ cord-303381-xvzhb7ix 36 6 endocytosis endocytosis NN cord-303381-xvzhb7ix 36 7 in in IN cord-303381-xvzhb7ix 36 8 different different JJ cord-303381-xvzhb7ix 36 9 manners manner NNS cord-303381-xvzhb7ix 36 10 . . . cord-303381-xvzhb7ix 37 1 Baf Baf NNP cord-303381-xvzhb7ix 37 2 A1 A1 NNP cord-303381-xvzhb7ix 37 3 is be VBZ cord-303381-xvzhb7ix 37 4 an an DT cord-303381-xvzhb7ix 37 5 antibiotic antibiotic NN cord-303381-xvzhb7ix 37 6 derived derive VBN cord-303381-xvzhb7ix 37 7 from from IN cord-303381-xvzhb7ix 37 8 Streptomyces Streptomyces NNP cord-303381-xvzhb7ix 37 9 griseus griseu NNS cord-303381-xvzhb7ix 37 10 and and CC cord-303381-xvzhb7ix 37 11 specifically specifically RB cord-303381-xvzhb7ix 37 12 inhibits inhibit VBZ cord-303381-xvzhb7ix 37 13 vacuolar vacuolar JJ cord-303381-xvzhb7ix 37 14 type type NN cord-303381-xvzhb7ix 37 15 H+ H+ NNP cord-303381-xvzhb7ix 38 1 ATPase ATPase NNP cord-303381-xvzhb7ix 38 2 [ [ -LRB- cord-303381-xvzhb7ix 38 3 23 23 CD cord-303381-xvzhb7ix 38 4 ] ] -RRB- cord-303381-xvzhb7ix 38 5 . . . cord-303381-xvzhb7ix 39 1 CPZ CPZ NNP cord-303381-xvzhb7ix 39 2 dislocates dislocate VBZ cord-303381-xvzhb7ix 39 3 clathrin clathrin NN cord-303381-xvzhb7ix 39 4 , , , cord-303381-xvzhb7ix 39 5 and and CC cord-303381-xvzhb7ix 39 6 its -PRON- PRP$ cord-303381-xvzhb7ix 39 7 adaptor adaptor NN cord-303381-xvzhb7ix 39 8 protein protein NN cord-303381-xvzhb7ix 39 9 from from IN cord-303381-xvzhb7ix 39 10 plasma plasma NN cord-303381-xvzhb7ix 39 11 membrane membrane NN cord-303381-xvzhb7ix 39 12 to to IN cord-303381-xvzhb7ix 39 13 cytosol cytosol NN cord-303381-xvzhb7ix 39 14 [ [ -LRB- cord-303381-xvzhb7ix 39 15 22 22 CD cord-303381-xvzhb7ix 39 16 ] ] -RRB- cord-303381-xvzhb7ix 40 1 and and CC cord-303381-xvzhb7ix 40 2 dynasore dynasore NN cord-303381-xvzhb7ix 40 3 inhibits inhibit VBZ cord-303381-xvzhb7ix 40 4 GTPase GTPase NNP cord-303381-xvzhb7ix 40 5 activity activity NN cord-303381-xvzhb7ix 40 6 of of IN cord-303381-xvzhb7ix 40 7 dynamin dynamin NN cord-303381-xvzhb7ix 40 8 specifically specifically RB cord-303381-xvzhb7ix 40 9 [ [ -LRB- cord-303381-xvzhb7ix 40 10 14 14 CD cord-303381-xvzhb7ix 40 11 ] ] -RRB- cord-303381-xvzhb7ix 40 12 . . . cord-303381-xvzhb7ix 41 1 All all DT cord-303381-xvzhb7ix 41 2 inhibitors inhibitor NNS cord-303381-xvzhb7ix 41 3 work work VBP cord-303381-xvzhb7ix 41 4 reversibly reversibly RB cord-303381-xvzhb7ix 41 5 , , , cord-303381-xvzhb7ix 41 6 and and CC cord-303381-xvzhb7ix 41 7 therefore therefore RB cord-303381-xvzhb7ix 41 8 , , , cord-303381-xvzhb7ix 41 9 endosome endosome NN cord-303381-xvzhb7ix 41 10 pathway pathway NN cord-303381-xvzhb7ix 41 11 can can MD cord-303381-xvzhb7ix 41 12 restart restart VB cord-303381-xvzhb7ix 41 13 once once RB cord-303381-xvzhb7ix 41 14 the the DT cord-303381-xvzhb7ix 41 15 drug drug NN cord-303381-xvzhb7ix 41 16 was be VBD cord-303381-xvzhb7ix 41 17 removed remove VBN cord-303381-xvzhb7ix 41 18 from from IN cord-303381-xvzhb7ix 41 19 the the DT cord-303381-xvzhb7ix 41 20 culture culture NN cord-303381-xvzhb7ix 41 21 . . . cord-303381-xvzhb7ix 42 1 HmLu-1 HmLu-1 NNP cord-303381-xvzhb7ix 42 2 cells cell NNS cord-303381-xvzhb7ix 42 3 prepared prepare VBN cord-303381-xvzhb7ix 42 4 in in IN cord-303381-xvzhb7ix 42 5 6 6 CD cord-303381-xvzhb7ix 42 6 well well NN cord-303381-xvzhb7ix 42 7 plate plate NN cord-303381-xvzhb7ix 42 8 were be VBD cord-303381-xvzhb7ix 42 9 treated treat VBN cord-303381-xvzhb7ix 42 10 with with IN cord-303381-xvzhb7ix 42 11 Dulbecco Dulbecco NNP cord-303381-xvzhb7ix 42 12 's 's POS cord-303381-xvzhb7ix 42 13 modified modify VBN cord-303381-xvzhb7ix 42 14 eagle eagle NNP cord-303381-xvzhb7ix 42 15 's 's POS cord-303381-xvzhb7ix 42 16 medium medium NN cord-303381-xvzhb7ix 42 17 ( ( -LRB- cord-303381-xvzhb7ix 42 18 DMEM DMEM NNP cord-303381-xvzhb7ix 42 19 , , , cord-303381-xvzhb7ix 42 20 Wako Wako NNP cord-303381-xvzhb7ix 42 21 ) ) -RRB- cord-303381-xvzhb7ix 42 22 containing contain VBG cord-303381-xvzhb7ix 42 23 various various JJ cord-303381-xvzhb7ix 42 24 concentrations concentration NNS cord-303381-xvzhb7ix 42 25 of of IN cord-303381-xvzhb7ix 42 26 inhibitors inhibitor NNS cord-303381-xvzhb7ix 42 27 for for IN cord-303381-xvzhb7ix 42 28 30 30 CD cord-303381-xvzhb7ix 42 29 min min NN cord-303381-xvzhb7ix 42 30 at at IN cord-303381-xvzhb7ix 42 31 37 37 CD cord-303381-xvzhb7ix 42 32 ° ° NN cord-303381-xvzhb7ix 42 33 C c NN cord-303381-xvzhb7ix 42 34 . . . cord-303381-xvzhb7ix 43 1 After after IN cord-303381-xvzhb7ix 43 2 cells cell NNS cord-303381-xvzhb7ix 43 3 were be VBD cord-303381-xvzhb7ix 43 4 chilled chill VBN cord-303381-xvzhb7ix 43 5 on on IN cord-303381-xvzhb7ix 43 6 ice ice NN cord-303381-xvzhb7ix 43 7 for for IN cord-303381-xvzhb7ix 43 8 5 5 CD cord-303381-xvzhb7ix 43 9 min min NN cord-303381-xvzhb7ix 43 10 , , , cord-303381-xvzhb7ix 43 11 the the DT cord-303381-xvzhb7ix 43 12 media medium NNS cord-303381-xvzhb7ix 43 13 were be VBD cord-303381-xvzhb7ix 43 14 removed remove VBN cord-303381-xvzhb7ix 43 15 , , , cord-303381-xvzhb7ix 43 16 and and CC cord-303381-xvzhb7ix 43 17 cells cell NNS cord-303381-xvzhb7ix 43 18 were be VBD cord-303381-xvzhb7ix 43 19 infected infect VBN cord-303381-xvzhb7ix 43 20 with with IN cord-303381-xvzhb7ix 43 21 IBAV IBAV NNP cord-303381-xvzhb7ix 43 22 at at IN cord-303381-xvzhb7ix 43 23 MOI=3 MOI=3 NNP cord-303381-xvzhb7ix 43 24 for for IN cord-303381-xvzhb7ix 43 25 1 1 CD cord-303381-xvzhb7ix 43 26 hr hr NN cord-303381-xvzhb7ix 43 27 at at IN cord-303381-xvzhb7ix 43 28 4 4 CD cord-303381-xvzhb7ix 43 29 ° ° NN cord-303381-xvzhb7ix 43 30 C c NN cord-303381-xvzhb7ix 43 31 . . . cord-303381-xvzhb7ix 44 1 After after IN cord-303381-xvzhb7ix 44 2 a a DT cord-303381-xvzhb7ix 44 3 wash wash NN cord-303381-xvzhb7ix 44 4 with with IN cord-303381-xvzhb7ix 44 5 PBS PBS NNP cord-303381-xvzhb7ix 44 6 ( ( -LRB- cord-303381-xvzhb7ix 44 7 − − NNP cord-303381-xvzhb7ix 44 8 ) ) -RRB- cord-303381-xvzhb7ix 44 9 , , , cord-303381-xvzhb7ix 44 10 cells cell NNS cord-303381-xvzhb7ix 44 11 were be VBD cord-303381-xvzhb7ix 44 12 further further RB cord-303381-xvzhb7ix 44 13 incubated incubate VBN cord-303381-xvzhb7ix 44 14 with with IN cord-303381-xvzhb7ix 44 15 DMEM DMEM NNP cord-303381-xvzhb7ix 44 16 plus plus CC cord-303381-xvzhb7ix 44 17 inhibitors inhibitor NNS cord-303381-xvzhb7ix 44 18 for for IN cord-303381-xvzhb7ix 44 19 30 30 CD cord-303381-xvzhb7ix 44 20 min min NN cord-303381-xvzhb7ix 44 21 at at IN cord-303381-xvzhb7ix 44 22 37 37 CD cord-303381-xvzhb7ix 44 23 ° ° NN cord-303381-xvzhb7ix 44 24 C c NN cord-303381-xvzhb7ix 44 25 , , , cord-303381-xvzhb7ix 44 26 and and CC cord-303381-xvzhb7ix 44 27 the the DT cord-303381-xvzhb7ix 44 28 media medium NNS cord-303381-xvzhb7ix 44 29 were be VBD cord-303381-xvzhb7ix 44 30 replaced replace VBN cord-303381-xvzhb7ix 44 31 with with IN cord-303381-xvzhb7ix 44 32 DMEM DMEM NNP cord-303381-xvzhb7ix 44 33 containing contain VBG cord-303381-xvzhb7ix 44 34 10 10 CD cord-303381-xvzhb7ix 44 35 % % NN cord-303381-xvzhb7ix 44 36 fetal fetal JJ cord-303381-xvzhb7ix 44 37 bovine bovine JJ cord-303381-xvzhb7ix 44 38 serum serum NN cord-303381-xvzhb7ix 44 39 ( ( -LRB- cord-303381-xvzhb7ix 44 40 10FDMEM 10fdmem CD cord-303381-xvzhb7ix 44 41 ) ) -RRB- cord-303381-xvzhb7ix 44 42 . . . cord-303381-xvzhb7ix 45 1 Cells cell NNS cord-303381-xvzhb7ix 45 2 were be VBD cord-303381-xvzhb7ix 45 3 incubated incubate VBN cord-303381-xvzhb7ix 45 4 for for IN cord-303381-xvzhb7ix 45 5 further further JJ cord-303381-xvzhb7ix 45 6 24 24 CD cord-303381-xvzhb7ix 45 7 hr hr NN cord-303381-xvzhb7ix 45 8 , , , cord-303381-xvzhb7ix 45 9 and and CC cord-303381-xvzhb7ix 45 10 culture culture NN cord-303381-xvzhb7ix 45 11 supernatant supernatant NN cord-303381-xvzhb7ix 45 12 was be VBD cord-303381-xvzhb7ix 45 13 collected collect VBN cord-303381-xvzhb7ix 45 14 . . . cord-303381-xvzhb7ix 46 1 Collected collect VBN cord-303381-xvzhb7ix 46 2 supernatant supernatant JJ cord-303381-xvzhb7ix 46 3 was be VBD cord-303381-xvzhb7ix 46 4 subjected subject VBN cord-303381-xvzhb7ix 46 5 to to IN cord-303381-xvzhb7ix 46 6 plaque plaque NN cord-303381-xvzhb7ix 46 7 assay assay NN cord-303381-xvzhb7ix 46 8 , , , cord-303381-xvzhb7ix 46 9 and and CC cord-303381-xvzhb7ix 46 10 after after IN cord-303381-xvzhb7ix 46 11 staining stain VBG cord-303381-xvzhb7ix 46 12 with with IN cord-303381-xvzhb7ix 46 13 crystal crystal JJ cord-303381-xvzhb7ix 46 14 violet violet NN cord-303381-xvzhb7ix 46 15 solution solution NN cord-303381-xvzhb7ix 46 16 ( ( -LRB- cord-303381-xvzhb7ix 46 17 0.1 0.1 CD cord-303381-xvzhb7ix 46 18 % % NN cord-303381-xvzhb7ix 46 19 crystal crystal NN cord-303381-xvzhb7ix 46 20 violet violet NN cord-303381-xvzhb7ix 46 21 in in IN cord-303381-xvzhb7ix 46 22 10 10 CD cord-303381-xvzhb7ix 46 23 % % NN cord-303381-xvzhb7ix 46 24 buffered buffer VBN cord-303381-xvzhb7ix 46 25 formalin formalin NN cord-303381-xvzhb7ix 46 26 and and CC cord-303381-xvzhb7ix 46 27 20 20 CD cord-303381-xvzhb7ix 46 28 % % NN cord-303381-xvzhb7ix 46 29 methanol methanol NN cord-303381-xvzhb7ix 46 30 ) ) -RRB- cord-303381-xvzhb7ix 46 31 , , , cord-303381-xvzhb7ix 46 32 the the DT cord-303381-xvzhb7ix 46 33 number number NN cord-303381-xvzhb7ix 46 34 of of IN cord-303381-xvzhb7ix 46 35 plaque plaque NN cord-303381-xvzhb7ix 46 36 was be VBD cord-303381-xvzhb7ix 46 37 counted count VBN cord-303381-xvzhb7ix 46 38 . . . cord-303381-xvzhb7ix 47 1 To to TO cord-303381-xvzhb7ix 47 2 analyze analyze VB cord-303381-xvzhb7ix 47 3 the the DT cord-303381-xvzhb7ix 47 4 statistical statistical JJ cord-303381-xvzhb7ix 47 5 significance significance NN cord-303381-xvzhb7ix 47 6 of of IN cord-303381-xvzhb7ix 47 7 each each DT cord-303381-xvzhb7ix 47 8 inhibitor inhibitor NN cord-303381-xvzhb7ix 47 9 concentration concentration NN cord-303381-xvzhb7ix 47 10 group group NN cord-303381-xvzhb7ix 47 11 against against IN cord-303381-xvzhb7ix 47 12 mock mock JJ cord-303381-xvzhb7ix 47 13 treated treated JJ cord-303381-xvzhb7ix 47 14 group group NN cord-303381-xvzhb7ix 47 15 , , , cord-303381-xvzhb7ix 47 16 statistical statistical JJ cord-303381-xvzhb7ix 47 17 software software NN cord-303381-xvzhb7ix 47 18 R r NN cord-303381-xvzhb7ix 47 19 [ [ -LRB- cord-303381-xvzhb7ix 47 20 16 16 CD cord-303381-xvzhb7ix 47 21 ] ] -RRB- cord-303381-xvzhb7ix 47 22 was be VBD cord-303381-xvzhb7ix 47 23 used use VBN cord-303381-xvzhb7ix 47 24 to to TO cord-303381-xvzhb7ix 47 25 run run VB cord-303381-xvzhb7ix 47 26 Dunnet Dunnet NNP cord-303381-xvzhb7ix 47 27 's 's POS cord-303381-xvzhb7ix 47 28 test test NN cord-303381-xvzhb7ix 47 29 [ [ -LRB- cord-303381-xvzhb7ix 47 30 7 7 CD cord-303381-xvzhb7ix 47 31 ] ] -RRB- cord-303381-xvzhb7ix 47 32 . . . cord-303381-xvzhb7ix 48 1 Figure figure NN cord-303381-xvzhb7ix 48 2 1a 1a CD cord-303381-xvzhb7ix 48 3 shows show VBZ cord-303381-xvzhb7ix 48 4 the the DT cord-303381-xvzhb7ix 48 5 number number NN cord-303381-xvzhb7ix 48 6 of of IN cord-303381-xvzhb7ix 48 7 infectious infectious JJ cord-303381-xvzhb7ix 48 8 IBAV ibav NN cord-303381-xvzhb7ix 48 9 in in IN cord-303381-xvzhb7ix 48 10 the the DT cord-303381-xvzhb7ix 48 11 supernatant supernatant NN cord-303381-xvzhb7ix 48 12 of of IN cord-303381-xvzhb7ix 48 13 the the DT cord-303381-xvzhb7ix 48 14 cells cell NNS cord-303381-xvzhb7ix 48 15 treated treat VBN cord-303381-xvzhb7ix 48 16 with with IN cord-303381-xvzhb7ix 48 17 Baf Baf NNP cord-303381-xvzhb7ix 48 18 A1 A1 NNP cord-303381-xvzhb7ix 48 19 , , , cord-303381-xvzhb7ix 48 20 CPZ CPZ NNP cord-303381-xvzhb7ix 48 21 or or CC cord-303381-xvzhb7ix 48 22 dynasore dynasore NN cord-303381-xvzhb7ix 48 23 . . . cord-303381-xvzhb7ix 49 1 All all DT cord-303381-xvzhb7ix 49 2 inhibitors inhibitor NNS cord-303381-xvzhb7ix 49 3 were be VBD cord-303381-xvzhb7ix 49 4 shown show VBN cord-303381-xvzhb7ix 49 5 to to TO cord-303381-xvzhb7ix 49 6 decrease decrease VB cord-303381-xvzhb7ix 49 7 virus virus NN cord-303381-xvzhb7ix 49 8 titer titer NN cord-303381-xvzhb7ix 49 9 when when WRB cord-303381-xvzhb7ix 49 10 their -PRON- PRP$ cord-303381-xvzhb7ix 49 11 concentration concentration NN cord-303381-xvzhb7ix 49 12 was be VBD cord-303381-xvzhb7ix 49 13 elevated elevate VBN cord-303381-xvzhb7ix 49 14 . . . cord-303381-xvzhb7ix 50 1 The the DT cord-303381-xvzhb7ix 50 2 most most RBS cord-303381-xvzhb7ix 50 3 significant significant JJ cord-303381-xvzhb7ix 50 4 decrease decrease NN cord-303381-xvzhb7ix 50 5 was be VBD cord-303381-xvzhb7ix 50 6 observed observe VBN cord-303381-xvzhb7ix 50 7 when when WRB cord-303381-xvzhb7ix 50 8 Baf Baf NNP cord-303381-xvzhb7ix 50 9 A1 A1 NNP cord-303381-xvzhb7ix 50 10 concentration concentration NN cord-303381-xvzhb7ix 50 11 was be VBD cord-303381-xvzhb7ix 50 12 higher high JJR cord-303381-xvzhb7ix 50 13 than than IN cord-303381-xvzhb7ix 50 14 6.25 6.25 CD cord-303381-xvzhb7ix 50 15 nM nM NNP cord-303381-xvzhb7ix 50 16 ( ( -LRB- cord-303381-xvzhb7ix 50 17 P=0.0013 P=0.0013 NNP cord-303381-xvzhb7ix 50 18 for for IN cord-303381-xvzhb7ix 50 19 6.25 6.25 CD cord-303381-xvzhb7ix 50 20 nM nm NN cord-303381-xvzhb7ix 50 21 against against IN cord-303381-xvzhb7ix 50 22 0 0 CD cord-303381-xvzhb7ix 50 23 nM nm NN cord-303381-xvzhb7ix 50 24 ) ) -RRB- cord-303381-xvzhb7ix 50 25 . . . cord-303381-xvzhb7ix 51 1 At at IN cord-303381-xvzhb7ix 51 2 the the DT cord-303381-xvzhb7ix 51 3 same same JJ cord-303381-xvzhb7ix 51 4 time time NN cord-303381-xvzhb7ix 51 5 , , , cord-303381-xvzhb7ix 51 6 the the DT cord-303381-xvzhb7ix 51 7 effect effect NN cord-303381-xvzhb7ix 51 8 of of IN cord-303381-xvzhb7ix 51 9 those those DT cord-303381-xvzhb7ix 51 10 inhibitors inhibitor NNS cord-303381-xvzhb7ix 51 11 to to IN cord-303381-xvzhb7ix 51 12 HmLu-1 HmLu-1 NNP cord-303381-xvzhb7ix 51 13 viability viability NN cord-303381-xvzhb7ix 51 14 was be VBD cord-303381-xvzhb7ix 51 15 tested test VBN cord-303381-xvzhb7ix 51 16 . . . cord-303381-xvzhb7ix 52 1 HmLu-1 HmLu-1 NNP cord-303381-xvzhb7ix 52 2 cells cell NNS cord-303381-xvzhb7ix 52 3 in in IN cord-303381-xvzhb7ix 52 4 96 96 CD cord-303381-xvzhb7ix 52 5 well well RB cord-303381-xvzhb7ix 52 6 multiwell multiwell NNP cord-303381-xvzhb7ix 52 7 plate plate NN cord-303381-xvzhb7ix 52 8 were be VBD cord-303381-xvzhb7ix 52 9 treated treat VBN cord-303381-xvzhb7ix 52 10 with with IN cord-303381-xvzhb7ix 52 11 media medium NNS cord-303381-xvzhb7ix 52 12 containing contain VBG cord-303381-xvzhb7ix 52 13 various various JJ cord-303381-xvzhb7ix 52 14 concentrations concentration NNS cord-303381-xvzhb7ix 52 15 of of IN cord-303381-xvzhb7ix 52 16 inhibitors inhibitor NNS cord-303381-xvzhb7ix 52 17 for for IN cord-303381-xvzhb7ix 52 18 1 1 CD cord-303381-xvzhb7ix 52 19 hr hr NN cord-303381-xvzhb7ix 52 20 without without IN cord-303381-xvzhb7ix 52 21 IBAV IBAV NNP cord-303381-xvzhb7ix 52 22 infection infection NN cord-303381-xvzhb7ix 52 23 . . . cord-303381-xvzhb7ix 53 1 After after IN cord-303381-xvzhb7ix 53 2 24 24 CD cord-303381-xvzhb7ix 53 3 hr hr NN cord-303381-xvzhb7ix 53 4 incubation incubation NN cord-303381-xvzhb7ix 53 5 , , , cord-303381-xvzhb7ix 53 6 the the DT cord-303381-xvzhb7ix 53 7 number number NN cord-303381-xvzhb7ix 53 8 of of IN cord-303381-xvzhb7ix 53 9 viable viable JJ cord-303381-xvzhb7ix 53 10 cells cell NNS cord-303381-xvzhb7ix 53 11 was be VBD cord-303381-xvzhb7ix 53 12 quantified quantify VBN cord-303381-xvzhb7ix 53 13 using use VBG cord-303381-xvzhb7ix 53 14 CellTiter CellTiter NNP cord-303381-xvzhb7ix 53 15 ® ® . cord-303381-xvzhb7ix 53 16 Aqueous Aqueous NNP cord-303381-xvzhb7ix 53 17 One one CD cord-303381-xvzhb7ix 53 18 Solution Solution NNP cord-303381-xvzhb7ix 53 19 Reagent Reagent NNP cord-303381-xvzhb7ix 53 20 ( ( -LRB- cord-303381-xvzhb7ix 53 21 Promega Promega NNP cord-303381-xvzhb7ix 53 22 , , , cord-303381-xvzhb7ix 53 23 Madison Madison NNP cord-303381-xvzhb7ix 53 24 , , , cord-303381-xvzhb7ix 53 25 WI WI NNP cord-303381-xvzhb7ix 53 26 , , , cord-303381-xvzhb7ix 53 27 U.S.A. U.S.A. NNP cord-303381-xvzhb7ix 53 28 ) ) -RRB- cord-303381-xvzhb7ix 53 29 . . . cord-303381-xvzhb7ix 54 1 As as IN cord-303381-xvzhb7ix 54 2 shown show VBN cord-303381-xvzhb7ix 54 3 in in IN cord-303381-xvzhb7ix 54 4 Fig Fig NNP cord-303381-xvzhb7ix 54 5 . . . cord-303381-xvzhb7ix 55 1 1b 1b LS cord-303381-xvzhb7ix 55 2 , , , cord-303381-xvzhb7ix 55 3 no no DT cord-303381-xvzhb7ix 55 4 inhibitors inhibitor NNS cord-303381-xvzhb7ix 55 5 showed show VBD cord-303381-xvzhb7ix 55 6 significant significant JJ cord-303381-xvzhb7ix 55 7 effect effect NN cord-303381-xvzhb7ix 55 8 on on IN cord-303381-xvzhb7ix 55 9 viable viable JJ cord-303381-xvzhb7ix 55 10 cell cell NN cord-303381-xvzhb7ix 55 11 numbers number NNS cord-303381-xvzhb7ix 55 12 . . . cord-303381-xvzhb7ix 56 1 These these DT cord-303381-xvzhb7ix 56 2 results result NNS cord-303381-xvzhb7ix 56 3 indicated indicate VBD cord-303381-xvzhb7ix 56 4 that that IN cord-303381-xvzhb7ix 56 5 IBAV IBAV NNP cord-303381-xvzhb7ix 56 6 utilizes utilize VBZ cord-303381-xvzhb7ix 56 7 clathrin clathrin NN cord-303381-xvzhb7ix 56 8 - - HYPH cord-303381-xvzhb7ix 56 9 dependent dependent JJ cord-303381-xvzhb7ix 56 10 endosomal endosomal NNP cord-303381-xvzhb7ix 56 11 pathway pathway NN cord-303381-xvzhb7ix 56 12 for for IN cord-303381-xvzhb7ix 56 13 infection infection NN cord-303381-xvzhb7ix 56 14 and and CC cord-303381-xvzhb7ix 56 15 coincided coincide VBD cord-303381-xvzhb7ix 56 16 with with IN cord-303381-xvzhb7ix 56 17 the the DT cord-303381-xvzhb7ix 56 18 previous previous JJ cord-303381-xvzhb7ix 56 19 research research NN cord-303381-xvzhb7ix 56 20 on on IN cord-303381-xvzhb7ix 56 21 bluetongue bluetongue NN cord-303381-xvzhb7ix 56 22 virus virus NN cord-303381-xvzhb7ix 56 23 entry entry NN cord-303381-xvzhb7ix 56 24 [ [ -LRB- cord-303381-xvzhb7ix 56 25 8 8 CD cord-303381-xvzhb7ix 56 26 ] ] -RRB- cord-303381-xvzhb7ix 56 27 . . . cord-303381-xvzhb7ix 57 1 To to TO cord-303381-xvzhb7ix 57 2 confirm confirm VB cord-303381-xvzhb7ix 57 3 the the DT cord-303381-xvzhb7ix 57 4 effect effect NN cord-303381-xvzhb7ix 57 5 of of IN cord-303381-xvzhb7ix 57 6 low low JJ cord-303381-xvzhb7ix 57 7 pH ph NN cord-303381-xvzhb7ix 57 8 on on IN cord-303381-xvzhb7ix 57 9 virus virus NN cord-303381-xvzhb7ix 57 10 infectivity infectivity NN cord-303381-xvzhb7ix 57 11 , , , cord-303381-xvzhb7ix 57 12 purified purify VBN cord-303381-xvzhb7ix 57 13 IBAV IBAV NNP cord-303381-xvzhb7ix 57 14 was be VBD cord-303381-xvzhb7ix 57 15 incubated incubate VBN cord-303381-xvzhb7ix 57 16 in in IN cord-303381-xvzhb7ix 57 17 PBS PBS NNP cord-303381-xvzhb7ix 57 18 ( ( -LRB- cord-303381-xvzhb7ix 57 19 − − NNP cord-303381-xvzhb7ix 57 20 ) ) -RRB- cord-303381-xvzhb7ix 57 21 with with IN cord-303381-xvzhb7ix 57 22 several several JJ cord-303381-xvzhb7ix 57 23 pH pH NNP cord-303381-xvzhb7ix 57 24 ( ( -LRB- cord-303381-xvzhb7ix 57 25 pH=4 pH=4 NNP cord-303381-xvzhb7ix 57 26 , , , cord-303381-xvzhb7ix 57 27 7 7 CD cord-303381-xvzhb7ix 57 28 or or CC cord-303381-xvzhb7ix 57 29 9 9 CD cord-303381-xvzhb7ix 57 30 ) ) -RRB- cord-303381-xvzhb7ix 57 31 for for IN cord-303381-xvzhb7ix 57 32 five five CD cord-303381-xvzhb7ix 57 33 min min NN cord-303381-xvzhb7ix 57 34 and and CC cord-303381-xvzhb7ix 57 35 infected infect VBN cord-303381-xvzhb7ix 57 36 to to IN cord-303381-xvzhb7ix 57 37 HmLu-1 HmLu-1 NNP cord-303381-xvzhb7ix 57 38 at at IN cord-303381-xvzhb7ix 57 39 MOI=0.01 MOI=0.01 NNP cord-303381-xvzhb7ix 57 40 . . . cord-303381-xvzhb7ix 58 1 We -PRON- PRP cord-303381-xvzhb7ix 58 2 employed employ VBD cord-303381-xvzhb7ix 58 3 lower low JJR cord-303381-xvzhb7ix 58 4 MOI MOI NNP cord-303381-xvzhb7ix 58 5 to to TO cord-303381-xvzhb7ix 58 6 protect protect VB cord-303381-xvzhb7ix 58 7 cells cell NNS cord-303381-xvzhb7ix 58 8 from from IN cord-303381-xvzhb7ix 58 9 severe severe JJ cord-303381-xvzhb7ix 58 10 CPE CPE NNP cord-303381-xvzhb7ix 58 11 at at IN cord-303381-xvzhb7ix 58 12 early early JJ cord-303381-xvzhb7ix 58 13 time time NN cord-303381-xvzhb7ix 58 14 point point NN cord-303381-xvzhb7ix 58 15 and and CC cord-303381-xvzhb7ix 58 16 achieve achieve VB cord-303381-xvzhb7ix 58 17 multi multi JJ cord-303381-xvzhb7ix 58 18 cycle cycle NN cord-303381-xvzhb7ix 58 19 infection infection NN cord-303381-xvzhb7ix 58 20 . . . cord-303381-xvzhb7ix 59 1 DMEM DMEM NNP cord-303381-xvzhb7ix 59 2 without without IN cord-303381-xvzhb7ix 59 3 FBS FBS NNP cord-303381-xvzhb7ix 59 4 was be VBD cord-303381-xvzhb7ix 59 5 used use VBN cord-303381-xvzhb7ix 59 6 for for IN cord-303381-xvzhb7ix 59 7 control control NN cord-303381-xvzhb7ix 59 8 incubation incubation NN cord-303381-xvzhb7ix 59 9 , , , cord-303381-xvzhb7ix 59 10 and and CC cord-303381-xvzhb7ix 59 11 the the DT cord-303381-xvzhb7ix 59 12 significance significance NN cord-303381-xvzhb7ix 59 13 in in IN cord-303381-xvzhb7ix 59 14 the the DT cord-303381-xvzhb7ix 59 15 difference difference NN cord-303381-xvzhb7ix 59 16 between between IN cord-303381-xvzhb7ix 59 17 control control NN cord-303381-xvzhb7ix 59 18 and and CC cord-303381-xvzhb7ix 59 19 each each DT cord-303381-xvzhb7ix 59 20 test test NN cord-303381-xvzhb7ix 59 21 group group NN cord-303381-xvzhb7ix 59 22 was be VBD cord-303381-xvzhb7ix 59 23 analyzed analyze VBN cord-303381-xvzhb7ix 59 24 by by IN cord-303381-xvzhb7ix 59 25 Dunnet Dunnet NNP cord-303381-xvzhb7ix 59 26 's 's POS cord-303381-xvzhb7ix 59 27 test test NN cord-303381-xvzhb7ix 59 28 . . . cord-303381-xvzhb7ix 60 1 As as IN cord-303381-xvzhb7ix 60 2 shown show VBN cord-303381-xvzhb7ix 60 3 in in IN cord-303381-xvzhb7ix 60 4 Fig Fig NNP cord-303381-xvzhb7ix 60 5 . . . cord-303381-xvzhb7ix 61 1 2a 2a LS cord-303381-xvzhb7ix 61 2 , , , cord-303381-xvzhb7ix 61 3 the the DT cord-303381-xvzhb7ix 61 4 pro- pro- JJ cord-303381-xvzhb7ix 61 5 Mouse Mouse NNP cord-303381-xvzhb7ix 61 6 antisera antisera NN cord-303381-xvzhb7ix 61 7 raised raise VBD cord-303381-xvzhb7ix 61 8 against against IN cord-303381-xvzhb7ix 61 9 VP2 VP2 NNP cord-303381-xvzhb7ix 61 10 ( ( -LRB- cord-303381-xvzhb7ix 61 11 anti anti JJ cord-303381-xvzhb7ix 61 12 - - JJ cord-303381-xvzhb7ix 61 13 VP2 vp2 JJ cord-303381-xvzhb7ix 61 14 ) ) -RRB- cord-303381-xvzhb7ix 61 15 or or CC cord-303381-xvzhb7ix 61 16 purified purify VBN cord-303381-xvzhb7ix 61 17 IBAV IBAV NNP cord-303381-xvzhb7ix 61 18 particle particle NN cord-303381-xvzhb7ix 61 19 ( ( -LRB- cord-303381-xvzhb7ix 61 20 anti anti JJ cord-303381-xvzhb7ix 61 21 - - JJ cord-303381-xvzhb7ix 61 22 IBAV IBAV NNP cord-303381-xvzhb7ix 61 23 ) ) -RRB- cord-303381-xvzhb7ix 61 24 were be VBD cord-303381-xvzhb7ix 61 25 used use VBN cord-303381-xvzhb7ix 61 26 as as IN cord-303381-xvzhb7ix 61 27 first first JJ cord-303381-xvzhb7ix 61 28 antibodies antibody NNS cord-303381-xvzhb7ix 61 29 . . . cord-303381-xvzhb7ix 62 1 duction duction NN cord-303381-xvzhb7ix 62 2 of of IN cord-303381-xvzhb7ix 62 3 progeny progeny JJ cord-303381-xvzhb7ix 62 4 virus virus NN cord-303381-xvzhb7ix 62 5 was be VBD cord-303381-xvzhb7ix 62 6 significantly significantly RB cord-303381-xvzhb7ix 62 7 suppressed suppress VBN cord-303381-xvzhb7ix 62 8 when when WRB cord-303381-xvzhb7ix 62 9 IBAV IBAV NNP cord-303381-xvzhb7ix 62 10 was be VBD cord-303381-xvzhb7ix 62 11 treated treat VBN cord-303381-xvzhb7ix 62 12 with with IN cord-303381-xvzhb7ix 62 13 PBS PBS NNP cord-303381-xvzhb7ix 62 14 ( ( -LRB- cord-303381-xvzhb7ix 62 15 pH4 ph4 NN cord-303381-xvzhb7ix 62 16 ) ) -RRB- cord-303381-xvzhb7ix 62 17 compared compare VBN cord-303381-xvzhb7ix 62 18 to to TO cord-303381-xvzhb7ix 62 19 control control NN cord-303381-xvzhb7ix 62 20 treatment treatment NN cord-303381-xvzhb7ix 62 21 ( ( -LRB- cord-303381-xvzhb7ix 62 22 P<0.001 P<0.001 NNP cord-303381-xvzhb7ix 62 23 ) ) -RRB- cord-303381-xvzhb7ix 62 24 . . . cord-303381-xvzhb7ix 63 1 Other other JJ cord-303381-xvzhb7ix 63 2 conditions condition NNS cord-303381-xvzhb7ix 63 3 did do VBD cord-303381-xvzhb7ix 63 4 not not RB cord-303381-xvzhb7ix 63 5 give give VB cord-303381-xvzhb7ix 63 6 any any DT cord-303381-xvzhb7ix 63 7 impact impact NN cord-303381-xvzhb7ix 63 8 on on IN cord-303381-xvzhb7ix 63 9 IBAV IBAV NNP cord-303381-xvzhb7ix 63 10 infectivity infectivity NN cord-303381-xvzhb7ix 63 11 ( ( -LRB- cord-303381-xvzhb7ix 63 12 pH7 pH7 NNP cord-303381-xvzhb7ix 63 13 and and CC cord-303381-xvzhb7ix 63 14 pH9 ph9 NN cord-303381-xvzhb7ix 63 15 , , , cord-303381-xvzhb7ix 63 16 P p NN cord-303381-xvzhb7ix 63 17 value value NN cord-303381-xvzhb7ix 63 18 was be VBD cord-303381-xvzhb7ix 63 19 0.993 0.993 CD cord-303381-xvzhb7ix 63 20 and and CC cord-303381-xvzhb7ix 63 21 0.906 0.906 CD cord-303381-xvzhb7ix 63 22 , , , cord-303381-xvzhb7ix 63 23 respectively respectively RB cord-303381-xvzhb7ix 63 24 ) ) -RRB- cord-303381-xvzhb7ix 63 25 . . . cord-303381-xvzhb7ix 64 1 The the DT cord-303381-xvzhb7ix 64 2 pH ph NN cord-303381-xvzhb7ix 64 3 of of IN cord-303381-xvzhb7ix 64 4 PBS PBS NNP cord-303381-xvzhb7ix 64 5 - - HYPH cord-303381-xvzhb7ix 64 6 IBAV IBAV NNP cord-303381-xvzhb7ix 64 7 mix mix NN cord-303381-xvzhb7ix 64 8 solution solution NN cord-303381-xvzhb7ix 64 9 was be VBD cord-303381-xvzhb7ix 64 10 confirmed confirm VBN cord-303381-xvzhb7ix 64 11 with with IN cord-303381-xvzhb7ix 64 12 pH pH NNP cord-303381-xvzhb7ix 64 13 test test NN cord-303381-xvzhb7ix 64 14 paper paper NN cord-303381-xvzhb7ix 64 15 after after IN cord-303381-xvzhb7ix 64 16 5 5 CD cord-303381-xvzhb7ix 64 17 min min NN cord-303381-xvzhb7ix 64 18 incubation incubation NN cord-303381-xvzhb7ix 64 19 ( ( -LRB- cord-303381-xvzhb7ix 64 20 data datum NNS cord-303381-xvzhb7ix 64 21 not not RB cord-303381-xvzhb7ix 64 22 shown show VBN cord-303381-xvzhb7ix 64 23 ) ) -RRB- cord-303381-xvzhb7ix 64 24 . . . cord-303381-xvzhb7ix 65 1 Many many JJ cord-303381-xvzhb7ix 65 2 enveloped envelop VBN cord-303381-xvzhb7ix 65 3 viruses virus NNS cord-303381-xvzhb7ix 65 4 lose lose VBP cord-303381-xvzhb7ix 65 5 its -PRON- PRP$ cord-303381-xvzhb7ix 65 6 infectivity infectivity NN cord-303381-xvzhb7ix 65 7 by by IN cord-303381-xvzhb7ix 65 8 low low JJ cord-303381-xvzhb7ix 65 9 pH pH NNP cord-303381-xvzhb7ix 65 10 treatment treatment NN cord-303381-xvzhb7ix 65 11 , , , cord-303381-xvzhb7ix 65 12 if if IN cord-303381-xvzhb7ix 65 13 they -PRON- PRP cord-303381-xvzhb7ix 65 14 utilize utilize VBP cord-303381-xvzhb7ix 65 15 endosomal endosomal NNP cord-303381-xvzhb7ix 65 16 acidification acidification NN cord-303381-xvzhb7ix 65 17 to to TO cord-303381-xvzhb7ix 65 18 trigger trigger VB cord-303381-xvzhb7ix 65 19 the the DT cord-303381-xvzhb7ix 65 20 activation activation NN cord-303381-xvzhb7ix 65 21 of of IN cord-303381-xvzhb7ix 65 22 their -PRON- PRP$ cord-303381-xvzhb7ix 65 23 protein protein NN cord-303381-xvzhb7ix 65 24 that that WDT cord-303381-xvzhb7ix 65 25 is be VBZ cord-303381-xvzhb7ix 65 26 responsible responsible JJ cord-303381-xvzhb7ix 65 27 for for IN cord-303381-xvzhb7ix 65 28 infection infection NN cord-303381-xvzhb7ix 65 29 [ [ -LRB- cord-303381-xvzhb7ix 65 30 15 15 CD cord-303381-xvzhb7ix 65 31 , , , cord-303381-xvzhb7ix 65 32 17 17 CD cord-303381-xvzhb7ix 65 33 , , , cord-303381-xvzhb7ix 65 34 18 18 CD cord-303381-xvzhb7ix 65 35 ] ] -RRB- cord-303381-xvzhb7ix 65 36 . . . cord-303381-xvzhb7ix 66 1 The the DT cord-303381-xvzhb7ix 66 2 result result NN cord-303381-xvzhb7ix 66 3 obtained obtain VBN cord-303381-xvzhb7ix 66 4 in in IN cord-303381-xvzhb7ix 66 5 this this DT cord-303381-xvzhb7ix 66 6 study study NN cord-303381-xvzhb7ix 66 7 was be VBD cord-303381-xvzhb7ix 66 8 consistent consistent JJ cord-303381-xvzhb7ix 66 9 with with IN cord-303381-xvzhb7ix 66 10 these these DT cord-303381-xvzhb7ix 66 11 observations observation NNS cord-303381-xvzhb7ix 66 12 suggesting suggest VBG cord-303381-xvzhb7ix 66 13 that that IN cord-303381-xvzhb7ix 66 14 low low JJ cord-303381-xvzhb7ix 66 15 pH pH NNP cord-303381-xvzhb7ix 66 16 treatment treatment NN cord-303381-xvzhb7ix 66 17 caused cause VBD cord-303381-xvzhb7ix 66 18 irreversible irreversible JJ cord-303381-xvzhb7ix 66 19 change change NN cord-303381-xvzhb7ix 66 20 on on IN cord-303381-xvzhb7ix 66 21 IBAV IBAV NNP cord-303381-xvzhb7ix 66 22 particle particle NN cord-303381-xvzhb7ix 66 23 that that WDT cord-303381-xvzhb7ix 66 24 leads lead VBZ cord-303381-xvzhb7ix 66 25 to to IN cord-303381-xvzhb7ix 66 26 the the DT cord-303381-xvzhb7ix 66 27 loss loss NN cord-303381-xvzhb7ix 66 28 of of IN cord-303381-xvzhb7ix 66 29 infectivity infectivity NN cord-303381-xvzhb7ix 66 30 . . . cord-303381-xvzhb7ix 67 1 To to TO cord-303381-xvzhb7ix 67 2 understand understand VB cord-303381-xvzhb7ix 67 3 the the DT cord-303381-xvzhb7ix 67 4 mechanism mechanism NN cord-303381-xvzhb7ix 67 5 of of IN cord-303381-xvzhb7ix 67 6 this this DT cord-303381-xvzhb7ix 67 7 loss loss NN cord-303381-xvzhb7ix 67 8 of of IN cord-303381-xvzhb7ix 67 9 infectivity infectivity NN cord-303381-xvzhb7ix 67 10 , , , cord-303381-xvzhb7ix 67 11 virus virus NN cord-303381-xvzhb7ix 67 12 particle particle NN cord-303381-xvzhb7ix 67 13 was be VBD cord-303381-xvzhb7ix 67 14 collected collect VBN cord-303381-xvzhb7ix 67 15 by by IN cord-303381-xvzhb7ix 67 16 ultracentrifugation ultracentrifugation NN cord-303381-xvzhb7ix 67 17 after after IN cord-303381-xvzhb7ix 67 18 low low JJ cord-303381-xvzhb7ix 67 19 pH pH NNP cord-303381-xvzhb7ix 67 20 treatment treatment NN cord-303381-xvzhb7ix 67 21 . . . cord-303381-xvzhb7ix 68 1 Western western JJ cord-303381-xvzhb7ix 68 2 blot blot NN cord-303381-xvzhb7ix 68 3 analysis analysis NN cord-303381-xvzhb7ix 68 4 using use VBG cord-303381-xvzhb7ix 68 5 anti anti JJ cord-303381-xvzhb7ix 68 6 - - JJ cord-303381-xvzhb7ix 68 7 VP2 vp2 JJ cord-303381-xvzhb7ix 68 8 antisera antisera NN cord-303381-xvzhb7ix 68 9 and and CC cord-303381-xvzhb7ix 68 10 anti anti JJ cord-303381-xvzhb7ix 68 11 - - JJ cord-303381-xvzhb7ix 68 12 IBAV ibav JJ cord-303381-xvzhb7ix 68 13 antisera antisera NNP cord-303381-xvzhb7ix 68 14 was be VBD cord-303381-xvzhb7ix 68 15 performed perform VBN cord-303381-xvzhb7ix 68 16 , , , cord-303381-xvzhb7ix 68 17 and and CC cord-303381-xvzhb7ix 68 18 VP2 vp2 NN cord-303381-xvzhb7ix 68 19 , , , cord-303381-xvzhb7ix 68 20 VP5 VP5 NNS cord-303381-xvzhb7ix 68 21 and and CC cord-303381-xvzhb7ix 68 22 VP7 vp7 NN cord-303381-xvzhb7ix 68 23 were be VBD cord-303381-xvzhb7ix 68 24 visualized visualize VBN cord-303381-xvzhb7ix 68 25 ( ( -LRB- cord-303381-xvzhb7ix 68 26 Fig Fig NNP cord-303381-xvzhb7ix 68 27 . . . cord-303381-xvzhb7ix 68 28 2b 2b NNP cord-303381-xvzhb7ix 68 29 ) ) -RRB- cord-303381-xvzhb7ix 68 30 . . . cord-303381-xvzhb7ix 69 1 The the DT cord-303381-xvzhb7ix 69 2 approximate approximate JJ cord-303381-xvzhb7ix 69 3 molecular molecular JJ cord-303381-xvzhb7ix 69 4 weights weight NNS cord-303381-xvzhb7ix 69 5 of of IN cord-303381-xvzhb7ix 69 6 VP2 vp2 NN cord-303381-xvzhb7ix 69 7 , , , cord-303381-xvzhb7ix 69 8 VP5 VP5 NNS cord-303381-xvzhb7ix 69 9 and and CC cord-303381-xvzhb7ix 69 10 VP7 vp7 NN cord-303381-xvzhb7ix 69 11 are be VBP cord-303381-xvzhb7ix 69 12 115 115 CD cord-303381-xvzhb7ix 69 13 , , , cord-303381-xvzhb7ix 69 14 60 60 CD cord-303381-xvzhb7ix 69 15 and and CC cord-303381-xvzhb7ix 69 16 38 38 CD cord-303381-xvzhb7ix 69 17 kDa kDa NNS cord-303381-xvzhb7ix 69 18 , , , cord-303381-xvzhb7ix 69 19 respectively respectively RB cord-303381-xvzhb7ix 69 20 . . . cord-303381-xvzhb7ix 70 1 In in IN cord-303381-xvzhb7ix 70 2 the the DT cord-303381-xvzhb7ix 70 3 case case NN cord-303381-xvzhb7ix 70 4 of of IN cord-303381-xvzhb7ix 70 5 BTV BTV NNP cord-303381-xvzhb7ix 70 6 , , , cord-303381-xvzhb7ix 70 7 it -PRON- PRP cord-303381-xvzhb7ix 70 8 was be VBD cord-303381-xvzhb7ix 70 9 shown show VBN cord-303381-xvzhb7ix 70 10 that that IN cord-303381-xvzhb7ix 70 11 VP2 vp2 NN cord-303381-xvzhb7ix 70 12 and and CC cord-303381-xvzhb7ix 70 13 VP5 VP5 NNP cord-303381-xvzhb7ix 70 14 form form VBP cord-303381-xvzhb7ix 70 15 outer outer JJ cord-303381-xvzhb7ix 70 16 capsid capsid NN cord-303381-xvzhb7ix 70 17 layer layer NN cord-303381-xvzhb7ix 70 18 and and CC cord-303381-xvzhb7ix 70 19 play play VB cord-303381-xvzhb7ix 70 20 important important JJ cord-303381-xvzhb7ix 70 21 roles role NNS cord-303381-xvzhb7ix 70 22 for for IN cord-303381-xvzhb7ix 70 23 the the DT cord-303381-xvzhb7ix 70 24 initial initial JJ cord-303381-xvzhb7ix 70 25 step step NN cord-303381-xvzhb7ix 70 26 of of IN cord-303381-xvzhb7ix 70 27 infection infection NN cord-303381-xvzhb7ix 70 28 [ [ -LRB- cord-303381-xvzhb7ix 70 29 24 24 CD cord-303381-xvzhb7ix 70 30 ] ] -RRB- cord-303381-xvzhb7ix 70 31 . . . cord-303381-xvzhb7ix 71 1 VP2 VP2 NNP cord-303381-xvzhb7ix 71 2 is be VBZ cord-303381-xvzhb7ix 71 3 a a DT cord-303381-xvzhb7ix 71 4 protein protein NN cord-303381-xvzhb7ix 71 5 reported report VBN cord-303381-xvzhb7ix 71 6 as as IN cord-303381-xvzhb7ix 71 7 a a DT cord-303381-xvzhb7ix 71 8 sialic sialic JJ cord-303381-xvzhb7ix 71 9 acid acid NN cord-303381-xvzhb7ix 71 10 binding bind VBG cord-303381-xvzhb7ix 71 11 protein protein NN cord-303381-xvzhb7ix 71 12 and and CC cord-303381-xvzhb7ix 71 13 implicated implicate VBN cord-303381-xvzhb7ix 71 14 to to TO cord-303381-xvzhb7ix 71 15 play play VB cord-303381-xvzhb7ix 71 16 a a DT cord-303381-xvzhb7ix 71 17 role role NN cord-303381-xvzhb7ix 71 18 in in IN cord-303381-xvzhb7ix 71 19 the the DT cord-303381-xvzhb7ix 71 20 attachment attachment NN cord-303381-xvzhb7ix 71 21 to to TO cord-303381-xvzhb7ix 71 22 target target VB cord-303381-xvzhb7ix 71 23 cells cell NNS cord-303381-xvzhb7ix 71 24 [ [ -LRB- cord-303381-xvzhb7ix 71 25 24 24 CD cord-303381-xvzhb7ix 71 26 ] ] -RRB- cord-303381-xvzhb7ix 71 27 . . . cord-303381-xvzhb7ix 72 1 VP5 VP5 NNP cord-303381-xvzhb7ix 72 2 is be VBZ cord-303381-xvzhb7ix 72 3 believed believe VBN cord-303381-xvzhb7ix 72 4 to to TO cord-303381-xvzhb7ix 72 5 work work VB cord-303381-xvzhb7ix 72 6 as as IN cord-303381-xvzhb7ix 72 7 a a DT cord-303381-xvzhb7ix 72 8 protein protein NN cord-303381-xvzhb7ix 72 9 which which WDT cord-303381-xvzhb7ix 72 10 disturbs disturb VBZ cord-303381-xvzhb7ix 72 11 the the DT cord-303381-xvzhb7ix 72 12 lipid lipid NN cord-303381-xvzhb7ix 72 13 bilayer bilayer NN cord-303381-xvzhb7ix 72 14 of of IN cord-303381-xvzhb7ix 72 15 target target NN cord-303381-xvzhb7ix 72 16 cells cell NNS cord-303381-xvzhb7ix 72 17 [ [ -LRB- cord-303381-xvzhb7ix 72 18 9 9 CD cord-303381-xvzhb7ix 72 19 ] ] -RRB- cord-303381-xvzhb7ix 72 20 . . . cord-303381-xvzhb7ix 73 1 As as IN cord-303381-xvzhb7ix 73 2 shown show VBN cord-303381-xvzhb7ix 73 3 in in IN cord-303381-xvzhb7ix 73 4 the the DT cord-303381-xvzhb7ix 73 5 left left JJ cord-303381-xvzhb7ix 73 6 panel panel NN cord-303381-xvzhb7ix 73 7 of of IN cord-303381-xvzhb7ix 73 8 Fig Fig NNP cord-303381-xvzhb7ix 73 9 . . . cord-303381-xvzhb7ix 74 1 2b 2b NNP cord-303381-xvzhb7ix 74 2 , , , cord-303381-xvzhb7ix 74 3 VP2 VP2 NNP cord-303381-xvzhb7ix 74 4 was be VBD cord-303381-xvzhb7ix 74 5 detected detect VBN cord-303381-xvzhb7ix 74 6 in in IN cord-303381-xvzhb7ix 74 7 the the DT cord-303381-xvzhb7ix 74 8 virus virus NN cord-303381-xvzhb7ix 74 9 treated treat VBN cord-303381-xvzhb7ix 74 10 with with IN cord-303381-xvzhb7ix 74 11 PBS PBS NNP cord-303381-xvzhb7ix 74 12 ( ( -LRB- cord-303381-xvzhb7ix 74 13 pH7 pH7 NNP cord-303381-xvzhb7ix 74 14 ) ) -RRB- cord-303381-xvzhb7ix 74 15 , , , cord-303381-xvzhb7ix 74 16 whereas whereas IN cord-303381-xvzhb7ix 74 17 no no DT cord-303381-xvzhb7ix 74 18 VP2 vp2 NN cord-303381-xvzhb7ix 74 19 was be VBD cord-303381-xvzhb7ix 74 20 detected detect VBN cord-303381-xvzhb7ix 74 21 in in IN cord-303381-xvzhb7ix 74 22 the the DT cord-303381-xvzhb7ix 74 23 virus virus NN cord-303381-xvzhb7ix 74 24 treated treat VBN cord-303381-xvzhb7ix 74 25 with with IN cord-303381-xvzhb7ix 74 26 PBS PBS NNP cord-303381-xvzhb7ix 74 27 ( ( -LRB- cord-303381-xvzhb7ix 74 28 pH4 ph4 NN cord-303381-xvzhb7ix 74 29 ) ) -RRB- cord-303381-xvzhb7ix 74 30 . . . cord-303381-xvzhb7ix 75 1 Similarly similarly RB cord-303381-xvzhb7ix 75 2 , , , cord-303381-xvzhb7ix 75 3 less less JJR cord-303381-xvzhb7ix 75 4 VP5 VP5 NNS cord-303381-xvzhb7ix 75 5 was be VBD cord-303381-xvzhb7ix 75 6 detected detect VBN cord-303381-xvzhb7ix 75 7 in in IN cord-303381-xvzhb7ix 75 8 the the DT cord-303381-xvzhb7ix 75 9 virus virus NN cord-303381-xvzhb7ix 75 10 treated treat VBN cord-303381-xvzhb7ix 75 11 with with IN cord-303381-xvzhb7ix 75 12 PBS PBS NNP cord-303381-xvzhb7ix 75 13 ( ( -LRB- cord-303381-xvzhb7ix 75 14 pH4 ph4 NN cord-303381-xvzhb7ix 75 15 ) ) -RRB- cord-303381-xvzhb7ix 75 16 compared compare VBN cord-303381-xvzhb7ix 75 17 to to IN cord-303381-xvzhb7ix 75 18 the the DT cord-303381-xvzhb7ix 75 19 virus virus NN cord-303381-xvzhb7ix 75 20 treated treat VBN cord-303381-xvzhb7ix 75 21 with with IN cord-303381-xvzhb7ix 75 22 PBS PBS NNP cord-303381-xvzhb7ix 75 23 ( ( -LRB- cord-303381-xvzhb7ix 75 24 pH7 ph7 NN cord-303381-xvzhb7ix 75 25 ) ) -RRB- cord-303381-xvzhb7ix 75 26 . . . cord-303381-xvzhb7ix 76 1 The the DT cord-303381-xvzhb7ix 76 2 amount amount NN cord-303381-xvzhb7ix 76 3 of of IN cord-303381-xvzhb7ix 76 4 VP7 vp7 NN cord-303381-xvzhb7ix 76 5 seemed seem VBD cord-303381-xvzhb7ix 76 6 to to TO cord-303381-xvzhb7ix 76 7 be be VB cord-303381-xvzhb7ix 76 8 the the DT cord-303381-xvzhb7ix 76 9 same same JJ cord-303381-xvzhb7ix 76 10 between between IN cord-303381-xvzhb7ix 76 11 PBS PBS NNP cord-303381-xvzhb7ix 76 12 ( ( -LRB- cord-303381-xvzhb7ix 76 13 pH4 ph4 NN cord-303381-xvzhb7ix 76 14 ) ) -RRB- cord-303381-xvzhb7ix 76 15 and and CC cord-303381-xvzhb7ix 76 16 PBS PBS NNP cord-303381-xvzhb7ix 76 17 ( ( -LRB- cord-303381-xvzhb7ix 76 18 pH7 pH7 NNP cord-303381-xvzhb7ix 76 19 ) ) -RRB- cord-303381-xvzhb7ix 76 20 , , , cord-303381-xvzhb7ix 76 21 and and CC cord-303381-xvzhb7ix 76 22 hence hence RB cord-303381-xvzhb7ix 76 23 , , , cord-303381-xvzhb7ix 76 24 it -PRON- PRP cord-303381-xvzhb7ix 76 25 was be VBD cord-303381-xvzhb7ix 76 26 suggested suggest VBN cord-303381-xvzhb7ix 76 27 that that IN cord-303381-xvzhb7ix 76 28 there there EX cord-303381-xvzhb7ix 76 29 are be VBP cord-303381-xvzhb7ix 76 30 the the DT cord-303381-xvzhb7ix 76 31 same same JJ cord-303381-xvzhb7ix 76 32 amounts amount NNS cord-303381-xvzhb7ix 76 33 of of IN cord-303381-xvzhb7ix 76 34 viral viral JJ cord-303381-xvzhb7ix 76 35 core core NN cord-303381-xvzhb7ix 76 36 in in IN cord-303381-xvzhb7ix 76 37 each each DT cord-303381-xvzhb7ix 76 38 sample sample NN cord-303381-xvzhb7ix 76 39 . . . cord-303381-xvzhb7ix 77 1 The the DT cord-303381-xvzhb7ix 77 2 result result NN cord-303381-xvzhb7ix 77 3 obtained obtain VBN cord-303381-xvzhb7ix 77 4 above above RB cord-303381-xvzhb7ix 77 5 implicated implicate VBD cord-303381-xvzhb7ix 77 6 that that IN cord-303381-xvzhb7ix 77 7 low low JJ cord-303381-xvzhb7ix 77 8 pH ph NN cord-303381-xvzhb7ix 77 9 treat treat NN cord-303381-xvzhb7ix 77 10 - - HYPH cord-303381-xvzhb7ix 77 11 ment ment NN cord-303381-xvzhb7ix 77 12 removes remove VBZ cord-303381-xvzhb7ix 77 13 IBAV IBAV NNP cord-303381-xvzhb7ix 77 14 outer outer JJ cord-303381-xvzhb7ix 77 15 capsid capsid NN cord-303381-xvzhb7ix 77 16 proteins protein NNS cord-303381-xvzhb7ix 77 17 from from IN cord-303381-xvzhb7ix 77 18 the the DT cord-303381-xvzhb7ix 77 19 particle particle NN cord-303381-xvzhb7ix 77 20 and and CC cord-303381-xvzhb7ix 77 21 initiates initiate VBZ cord-303381-xvzhb7ix 77 22 its -PRON- PRP$ cord-303381-xvzhb7ix 77 23 infection infection NN cord-303381-xvzhb7ix 77 24 . . . cord-303381-xvzhb7ix 78 1 Figure figure NN cord-303381-xvzhb7ix 78 2 3b 3b CD cord-303381-xvzhb7ix 78 3 shows show VBZ cord-303381-xvzhb7ix 78 4 the the DT cord-303381-xvzhb7ix 78 5 viability viability NN cord-303381-xvzhb7ix 78 6 of of IN cord-303381-xvzhb7ix 78 7 HmLu-1 HmLu-1 NNP cord-303381-xvzhb7ix 78 8 after after IN cord-303381-xvzhb7ix 78 9 a a DT cord-303381-xvzhb7ix 78 10 series series NN cord-303381-xvzhb7ix 78 11 of of IN cord-303381-xvzhb7ix 78 12 treatment treatment NN cord-303381-xvzhb7ix 78 13 without without IN cord-303381-xvzhb7ix 78 14 virus virus NN cord-303381-xvzhb7ix 78 15 infection infection NN cord-303381-xvzhb7ix 78 16 , , , cord-303381-xvzhb7ix 78 17 and and CC cord-303381-xvzhb7ix 78 18 no no DT cord-303381-xvzhb7ix 78 19 significant significant JJ cord-303381-xvzhb7ix 78 20 difference difference NN cord-303381-xvzhb7ix 78 21 was be VBD cord-303381-xvzhb7ix 78 22 observed observe VBN cord-303381-xvzhb7ix 78 23 ( ( -LRB- cord-303381-xvzhb7ix 78 24 P=0.983 P=0.983 NNS cord-303381-xvzhb7ix 78 25 , , , cord-303381-xvzhb7ix 78 26 Welch Welch NNP cord-303381-xvzhb7ix 78 27 's 's POS cord-303381-xvzhb7ix 78 28 t t NN cord-303381-xvzhb7ix 78 29 - - HYPH cord-303381-xvzhb7ix 78 30 test test NN cord-303381-xvzhb7ix 78 31 ) ) -RRB- cord-303381-xvzhb7ix 78 32 . . . cord-303381-xvzhb7ix 79 1 Results result NNS cord-303381-xvzhb7ix 79 2 obtained obtain VBN cord-303381-xvzhb7ix 79 3 in in IN cord-303381-xvzhb7ix 79 4 this this DT cord-303381-xvzhb7ix 79 5 approach approach NN cord-303381-xvzhb7ix 79 6 confirmed confirm VBD cord-303381-xvzhb7ix 79 7 the the DT cord-303381-xvzhb7ix 79 8 usage usage NN cord-303381-xvzhb7ix 79 9 of of IN cord-303381-xvzhb7ix 79 10 clathrin clathrin NN cord-303381-xvzhb7ix 79 11 - - HYPH cord-303381-xvzhb7ix 79 12 dependent dependent JJ cord-303381-xvzhb7ix 79 13 endocytosis endocytosis NN cord-303381-xvzhb7ix 79 14 pathway pathway NN cord-303381-xvzhb7ix 79 15 by by IN cord-303381-xvzhb7ix 79 16 orbivirus orbivirus NNP cord-303381-xvzhb7ix 79 17 and and CC cord-303381-xvzhb7ix 79 18 support support VB cord-303381-xvzhb7ix 79 19 the the DT cord-303381-xvzhb7ix 79 20 previous previous JJ cord-303381-xvzhb7ix 79 21 report report NN cord-303381-xvzhb7ix 79 22 [ [ -LRB- cord-303381-xvzhb7ix 79 23 8 8 CD cord-303381-xvzhb7ix 79 24 ] ] -RRB- cord-303381-xvzhb7ix 79 25 . . . cord-303381-xvzhb7ix 80 1 In in IN cord-303381-xvzhb7ix 80 2 addition addition NN cord-303381-xvzhb7ix 80 3 , , , cord-303381-xvzhb7ix 80 4 it -PRON- PRP cord-303381-xvzhb7ix 80 5 was be VBD cord-303381-xvzhb7ix 80 6 proved prove VBN cord-303381-xvzhb7ix 80 7 that that IN cord-303381-xvzhb7ix 80 8 the the DT cord-303381-xvzhb7ix 80 9 exposure exposure NN cord-303381-xvzhb7ix 80 10 to to IN cord-303381-xvzhb7ix 80 11 low low JJ cord-303381-xvzhb7ix 80 12 pH pH NNP cord-303381-xvzhb7ix 80 13 environment environment NN cord-303381-xvzhb7ix 80 14 is be VBZ cord-303381-xvzhb7ix 80 15 the the DT cord-303381-xvzhb7ix 80 16 essential essential JJ cord-303381-xvzhb7ix 80 17 condition condition NN cord-303381-xvzhb7ix 80 18 to to TO cord-303381-xvzhb7ix 80 19 initiate initiate VB cord-303381-xvzhb7ix 80 20 IBAV IBAV NNP cord-303381-xvzhb7ix 80 21 infection infection NN cord-303381-xvzhb7ix 80 22 . . . cord-303381-xvzhb7ix 81 1 In in IN cord-303381-xvzhb7ix 81 2 the the DT cord-303381-xvzhb7ix 81 3 presence presence NN cord-303381-xvzhb7ix 81 4 of of IN cord-303381-xvzhb7ix 81 5 Baf Baf NNP cord-303381-xvzhb7ix 81 6 A1 A1 NNP cord-303381-xvzhb7ix 81 7 , , , cord-303381-xvzhb7ix 81 8 the the DT cord-303381-xvzhb7ix 81 9 acidification acidification NN cord-303381-xvzhb7ix 81 10 of of IN cord-303381-xvzhb7ix 81 11 late late JJ cord-303381-xvzhb7ix 81 12 endosome endosome NN cord-303381-xvzhb7ix 81 13 , , , cord-303381-xvzhb7ix 81 14 as as RB cord-303381-xvzhb7ix 81 15 well well RB cord-303381-xvzhb7ix 81 16 as as IN cord-303381-xvzhb7ix 81 17 its -PRON- PRP$ cord-303381-xvzhb7ix 81 18 fusion fusion NN cord-303381-xvzhb7ix 81 19 to to IN cord-303381-xvzhb7ix 81 20 lysosome lysosome NN cord-303381-xvzhb7ix 81 21 is be VBZ cord-303381-xvzhb7ix 81 22 impaired impair VBN cord-303381-xvzhb7ix 81 23 [ [ -LRB- cord-303381-xvzhb7ix 81 24 20 20 CD cord-303381-xvzhb7ix 81 25 , , , cord-303381-xvzhb7ix 81 26 21 21 CD cord-303381-xvzhb7ix 81 27 ] ] -RRB- cord-303381-xvzhb7ix 81 28 . . . cord-303381-xvzhb7ix 82 1 Although although IN cord-303381-xvzhb7ix 82 2 the the DT cord-303381-xvzhb7ix 82 3 cell cell NN cord-303381-xvzhb7ix 82 4 surface surface NN cord-303381-xvzhb7ix 82 5 viruses virus NNS cord-303381-xvzhb7ix 82 6 could could MD cord-303381-xvzhb7ix 82 7 be be VB cord-303381-xvzhb7ix 82 8 internalized internalize VBN cord-303381-xvzhb7ix 82 9 during during IN cord-303381-xvzhb7ix 82 10 the the DT cord-303381-xvzhb7ix 82 11 post post JJ cord-303381-xvzhb7ix 82 12 - - NN cord-303381-xvzhb7ix 82 13 treatment treatment NN cord-303381-xvzhb7ix 82 14 , , , cord-303381-xvzhb7ix 82 15 they -PRON- PRP cord-303381-xvzhb7ix 82 16 can can MD cord-303381-xvzhb7ix 82 17 not not RB cord-303381-xvzhb7ix 82 18 be be VB cord-303381-xvzhb7ix 82 19 exposed expose VBN cord-303381-xvzhb7ix 82 20 to to IN cord-303381-xvzhb7ix 82 21 low low JJ cord-303381-xvzhb7ix 82 22 pH pH NNP cord-303381-xvzhb7ix 82 23 environment environment NN cord-303381-xvzhb7ix 82 24 in in IN cord-303381-xvzhb7ix 82 25 the the DT cord-303381-xvzhb7ix 82 26 presence presence NN cord-303381-xvzhb7ix 82 27 of of IN cord-303381-xvzhb7ix 82 28 Baf Baf NNP cord-303381-xvzhb7ix 82 29 A1 A1 NNP cord-303381-xvzhb7ix 82 30 . . . cord-303381-xvzhb7ix 83 1 However however RB cord-303381-xvzhb7ix 83 2 , , , cord-303381-xvzhb7ix 83 3 if if IN cord-303381-xvzhb7ix 83 4 viruses virus NNS cord-303381-xvzhb7ix 83 5 were be VBD cord-303381-xvzhb7ix 83 6 exposed expose VBN cord-303381-xvzhb7ix 83 7 to to IN cord-303381-xvzhb7ix 83 8 low low JJ cord-303381-xvzhb7ix 83 9 pH ph NN cord-303381-xvzhb7ix 83 10 at at IN cord-303381-xvzhb7ix 83 11 the the DT cord-303381-xvzhb7ix 83 12 cell cell NN cord-303381-xvzhb7ix 83 13 surface surface NN cord-303381-xvzhb7ix 83 14 before before IN cord-303381-xvzhb7ix 83 15 internalization internalization NN cord-303381-xvzhb7ix 83 16 , , , cord-303381-xvzhb7ix 83 17 they -PRON- PRP cord-303381-xvzhb7ix 83 18 acquired acquire VBD cord-303381-xvzhb7ix 83 19 the the DT cord-303381-xvzhb7ix 83 20 infectivity infectivity NN cord-303381-xvzhb7ix 83 21 and and CC cord-303381-xvzhb7ix 83 22 could could MD cord-303381-xvzhb7ix 83 23 start start VB cord-303381-xvzhb7ix 83 24 infection infection NN cord-303381-xvzhb7ix 83 25 under under IN cord-303381-xvzhb7ix 83 26 the the DT cord-303381-xvzhb7ix 83 27 effect effect NN cord-303381-xvzhb7ix 83 28 of of IN cord-303381-xvzhb7ix 83 29 Baf Baf NNP cord-303381-xvzhb7ix 83 30 A1 A1 NNP cord-303381-xvzhb7ix 83 31 . . . cord-303381-xvzhb7ix 84 1 PBS PBS NNP cord-303381-xvzhb7ix 84 2 ( ( -LRB- cord-303381-xvzhb7ix 84 3 pH4 ph4 NN cord-303381-xvzhb7ix 84 4 ) ) -RRB- cord-303381-xvzhb7ix 84 5 failed fail VBD cord-303381-xvzhb7ix 84 6 to to TO cord-303381-xvzhb7ix 84 7 enhance enhance VB cord-303381-xvzhb7ix 84 8 IBAV IBAV NNP cord-303381-xvzhb7ix 84 9 infection infection NN cord-303381-xvzhb7ix 84 10 under under IN cord-303381-xvzhb7ix 84 11 the the DT cord-303381-xvzhb7ix 84 12 influence influence NN cord-303381-xvzhb7ix 84 13 of of IN cord-303381-xvzhb7ix 84 14 other other JJ cord-303381-xvzhb7ix 84 15 two two CD cord-303381-xvzhb7ix 84 16 inhibitors inhibitor NNS cord-303381-xvzhb7ix 84 17 . . . cord-303381-xvzhb7ix 85 1 Since since IN cord-303381-xvzhb7ix 85 2 those those DT cord-303381-xvzhb7ix 85 3 two two CD cord-303381-xvzhb7ix 85 4 inhibitors inhibitor NNS cord-303381-xvzhb7ix 85 5 interrupt interrupt VBP cord-303381-xvzhb7ix 85 6 endosome endosome NN cord-303381-xvzhb7ix 85 7 pathway pathway NN cord-303381-xvzhb7ix 85 8 at at IN cord-303381-xvzhb7ix 85 9 the the DT cord-303381-xvzhb7ix 85 10 very very JJ cord-303381-xvzhb7ix 85 11 beginning beginning NN cord-303381-xvzhb7ix 85 12 , , , cord-303381-xvzhb7ix 85 13 no no DT cord-303381-xvzhb7ix 85 14 IBAV IBAV NNP cord-303381-xvzhb7ix 85 15 could could MD cord-303381-xvzhb7ix 85 16 be be VB cord-303381-xvzhb7ix 85 17 incorporated incorporate VBN cord-303381-xvzhb7ix 85 18 during during IN cord-303381-xvzhb7ix 85 19 post post JJ cord-303381-xvzhb7ix 85 20 - - JJ cord-303381-xvzhb7ix 85 21 treatment treatment NN cord-303381-xvzhb7ix 85 22 . . . cord-303381-xvzhb7ix 86 1 This this DT cord-303381-xvzhb7ix 86 2 might may MD cord-303381-xvzhb7ix 86 3 make make VB cord-303381-xvzhb7ix 86 4 both both DT cord-303381-xvzhb7ix 86 5 IBAV IBAV NNP cord-303381-xvzhb7ix 86 6 groups group NNS cord-303381-xvzhb7ix 86 7 to to TO cord-303381-xvzhb7ix 86 8 start start VB cord-303381-xvzhb7ix 86 9 infection infection NN cord-303381-xvzhb7ix 86 10 at at IN cord-303381-xvzhb7ix 86 11 the the DT cord-303381-xvzhb7ix 86 12 same same JJ cord-303381-xvzhb7ix 86 13 time time NN cord-303381-xvzhb7ix 86 14 and and CC cord-303381-xvzhb7ix 86 15 resulted result VBD cord-303381-xvzhb7ix 86 16 in in IN cord-303381-xvzhb7ix 86 17 the the DT cord-303381-xvzhb7ix 86 18 same same JJ cord-303381-xvzhb7ix 86 19 viral viral JJ cord-303381-xvzhb7ix 86 20 titer titer NN cord-303381-xvzhb7ix 86 21 . . . cord-303381-xvzhb7ix 87 1 Since since IN cord-303381-xvzhb7ix 87 2 we -PRON- PRP cord-303381-xvzhb7ix 87 3 could could MD cord-303381-xvzhb7ix 87 4 not not RB cord-303381-xvzhb7ix 87 5 deny deny VB cord-303381-xvzhb7ix 87 6 an an DT cord-303381-xvzhb7ix 87 7 involvement involvement NN cord-303381-xvzhb7ix 87 8 of of IN cord-303381-xvzhb7ix 87 9 any any DT cord-303381-xvzhb7ix 87 10 proteases protease NNS cord-303381-xvzhb7ix 87 11 , , , cord-303381-xvzhb7ix 87 12 additional additional JJ cord-303381-xvzhb7ix 87 13 research research NN cord-303381-xvzhb7ix 87 14 will will MD cord-303381-xvzhb7ix 87 15 be be VB cord-303381-xvzhb7ix 87 16 required require VBN cord-303381-xvzhb7ix 87 17 to to TO cord-303381-xvzhb7ix 87 18 reveal reveal VB cord-303381-xvzhb7ix 87 19 the the DT cord-303381-xvzhb7ix 87 20 entire entire JJ cord-303381-xvzhb7ix 87 21 molecular molecular JJ cord-303381-xvzhb7ix 87 22 mechanism mechanism NN cord-303381-xvzhb7ix 87 23 of of IN cord-303381-xvzhb7ix 87 24 orbivirus orbivirus NN cord-303381-xvzhb7ix 87 25 infection infection NN cord-303381-xvzhb7ix 87 26 . . . cord-303381-xvzhb7ix 88 1 The the DT cord-303381-xvzhb7ix 88 2 importance importance NN cord-303381-xvzhb7ix 88 3 of of IN cord-303381-xvzhb7ix 88 4 low low JJ cord-303381-xvzhb7ix 88 5 pH pH NNP cord-303381-xvzhb7ix 88 6 environment environment NN cord-303381-xvzhb7ix 88 7 for for IN cord-303381-xvzhb7ix 88 8 IBAV IBAV NNP cord-303381-xvzhb7ix 88 9 activation activation NN cord-303381-xvzhb7ix 88 10 that that WDT cord-303381-xvzhb7ix 88 11 we -PRON- PRP cord-303381-xvzhb7ix 88 12 proved prove VBD cord-303381-xvzhb7ix 88 13 in in IN cord-303381-xvzhb7ix 88 14 this this DT cord-303381-xvzhb7ix 88 15 study study NN cord-303381-xvzhb7ix 88 16 will will MD cord-303381-xvzhb7ix 88 17 help help VB cord-303381-xvzhb7ix 88 18 to to TO cord-303381-xvzhb7ix 88 19 design design VB cord-303381-xvzhb7ix 88 20 experimental experimental JJ cord-303381-xvzhb7ix 88 21 approach approach NN cord-303381-xvzhb7ix 88 22 for for IN cord-303381-xvzhb7ix 88 23 further further JJ cord-303381-xvzhb7ix 88 24 studies study NNS cord-303381-xvzhb7ix 88 25 . . . cord-303381-xvzhb7ix 89 1 Proteolytic proteolytic JJ cord-303381-xvzhb7ix 89 2 disassembly disassembly NN cord-303381-xvzhb7ix 89 3 is be VBZ cord-303381-xvzhb7ix 89 4 a a DT cord-303381-xvzhb7ix 89 5 critical critical JJ cord-303381-xvzhb7ix 89 6 determinant determinant NN cord-303381-xvzhb7ix 89 7 for for IN cord-303381-xvzhb7ix 89 8 reovirus reovirus NNP cord-303381-xvzhb7ix 89 9 oncolysis oncolysis NNP cord-303381-xvzhb7ix 89 10 Rotavirus Rotavirus NNP cord-303381-xvzhb7ix 89 11 cell cell NN cord-303381-xvzhb7ix 89 12 entry entry NN cord-303381-xvzhb7ix 89 13 Intraluminal intraluminal JJ cord-303381-xvzhb7ix 89 14 proteolytic proteolytic JJ cord-303381-xvzhb7ix 89 15 activation activation NN cord-303381-xvzhb7ix 89 16 plays play VBZ cord-303381-xvzhb7ix 89 17 an an DT cord-303381-xvzhb7ix 89 18 important important JJ cord-303381-xvzhb7ix 89 19 role role NN cord-303381-xvzhb7ix 89 20 in in IN cord-303381-xvzhb7ix 89 21 replication replication NN cord-303381-xvzhb7ix 89 22 of of IN cord-303381-xvzhb7ix 89 23 type type NN cord-303381-xvzhb7ix 89 24 1 1 CD cord-303381-xvzhb7ix 89 25 reovirus reovirus NN cord-303381-xvzhb7ix 89 26 in in IN cord-303381-xvzhb7ix 89 27 the the DT cord-303381-xvzhb7ix 89 28 intestines intestine NNS cord-303381-xvzhb7ix 89 29 of of IN cord-303381-xvzhb7ix 89 30 neonatal neonatal JJ cord-303381-xvzhb7ix 89 31 mice mouse NNS cord-303381-xvzhb7ix 90 1 Proteolytic proteolytic JJ cord-303381-xvzhb7ix 90 2 digestion digestion NN cord-303381-xvzhb7ix 90 3 of of IN cord-303381-xvzhb7ix 90 4 reovirus reovirus NNP cord-303381-xvzhb7ix 90 5 in in IN cord-303381-xvzhb7ix 90 6 the the DT cord-303381-xvzhb7ix 90 7 intestinal intestinal JJ cord-303381-xvzhb7ix 90 8 lumens lumen NNS cord-303381-xvzhb7ix 90 9 of of IN cord-303381-xvzhb7ix 90 10 neonatal neonatal JJ cord-303381-xvzhb7ix 90 11 mice mouse NNS cord-303381-xvzhb7ix 91 1 The the DT cord-303381-xvzhb7ix 91 2 structural structural JJ cord-303381-xvzhb7ix 91 3 biology biology NN cord-303381-xvzhb7ix 91 4 of of IN cord-303381-xvzhb7ix 91 5 type type NN cord-303381-xvzhb7ix 91 6 I -PRON- PRP cord-303381-xvzhb7ix 91 7 viral viral JJ cord-303381-xvzhb7ix 91 8 membrane membrane NN cord-303381-xvzhb7ix 91 9 fusion fusion NN cord-303381-xvzhb7ix 91 10 Virus Virus NNP cord-303381-xvzhb7ix 91 11 entry entry NN cord-303381-xvzhb7ix 91 12 : : : cord-303381-xvzhb7ix 91 13 molecular molecular JJ cord-303381-xvzhb7ix 91 14 mechanisms mechanism NNS cord-303381-xvzhb7ix 91 15 and and CC cord-303381-xvzhb7ix 91 16 biomedical biomedical JJ cord-303381-xvzhb7ix 91 17 applications application NNS cord-303381-xvzhb7ix 92 1 A a DT cord-303381-xvzhb7ix 92 2 multiple multiple JJ cord-303381-xvzhb7ix 92 3 comparison comparison NN cord-303381-xvzhb7ix 92 4 procedure procedure NN cord-303381-xvzhb7ix 92 5 for for IN cord-303381-xvzhb7ix 92 6 comparing compare VBG cord-303381-xvzhb7ix 92 7 several several JJ cord-303381-xvzhb7ix 92 8 treatments treatment NNS cord-303381-xvzhb7ix 92 9 with with IN cord-303381-xvzhb7ix 92 10 a a DT cord-303381-xvzhb7ix 92 11 control control NN cord-303381-xvzhb7ix 92 12 Bluetongue Bluetongue NNP cord-303381-xvzhb7ix 92 13 virus virus NN cord-303381-xvzhb7ix 92 14 entry entry NN cord-303381-xvzhb7ix 92 15 into into IN cord-303381-xvzhb7ix 92 16 cells cell NNS cord-303381-xvzhb7ix 93 1 A a DT cord-303381-xvzhb7ix 93 2 capsid capsid JJ cord-303381-xvzhb7ix 93 3 protein protein NN cord-303381-xvzhb7ix 93 4 of of IN cord-303381-xvzhb7ix 93 5 nonenveloped nonenveloped JJ cord-303381-xvzhb7ix 93 6 Bluetongue Bluetongue NNP cord-303381-xvzhb7ix 93 7 virus virus NN cord-303381-xvzhb7ix 93 8 exhibits exhibit VBZ cord-303381-xvzhb7ix 94 1 membrane membrane NN cord-303381-xvzhb7ix 94 2 fusion fusion NN cord-303381-xvzhb7ix 94 3 activity activity NN cord-303381-xvzhb7ix 94 4 Addition addition NN cord-303381-xvzhb7ix 94 5 of of IN cord-303381-xvzhb7ix 94 6 exogenous exogenous JJ cord-303381-xvzhb7ix 94 7 protease protease NN cord-303381-xvzhb7ix 94 8 facilitates facilitate VBZ cord-303381-xvzhb7ix 94 9 reovirus reovirus JJ cord-303381-xvzhb7ix 94 10 infection infection NN cord-303381-xvzhb7ix 94 11 in in IN cord-303381-xvzhb7ix 94 12 many many JJ cord-303381-xvzhb7ix 94 13 restrictive restrictive JJ cord-303381-xvzhb7ix 94 14 cells cell NNS cord-303381-xvzhb7ix 95 1 The the DT cord-303381-xvzhb7ix 95 2 orbivirus orbivirus NN cord-303381-xvzhb7ix 95 3 genus genus NN cord-303381-xvzhb7ix 95 4 . . . cord-303381-xvzhb7ix 96 1 Diversity diversity NN cord-303381-xvzhb7ix 96 2 , , , cord-303381-xvzhb7ix 96 3 structure structure NN cord-303381-xvzhb7ix 96 4 , , , cord-303381-xvzhb7ix 96 5 replication replication NN cord-303381-xvzhb7ix 96 6 and and CC cord-303381-xvzhb7ix 96 7 phylogenetic phylogenetic JJ cord-303381-xvzhb7ix 96 8 relationships relationship NNS cord-303381-xvzhb7ix 97 1 Ready ready JJ cord-303381-xvzhb7ix 97 2 , , , cord-303381-xvzhb7ix 97 3 set set VBN cord-303381-xvzhb7ix 97 4 , , , cord-303381-xvzhb7ix 97 5 fuse fuse NN cord-303381-xvzhb7ix 97 6 ! ! . cord-303381-xvzhb7ix 98 1 The the DT cord-303381-xvzhb7ix 98 2 coronavirus coronavirus NN cord-303381-xvzhb7ix 98 3 spike spike NN cord-303381-xvzhb7ix 98 4 protein protein NN cord-303381-xvzhb7ix 98 5 and and CC cord-303381-xvzhb7ix 98 6 acquisition acquisition NN cord-303381-xvzhb7ix 98 7 of of IN cord-303381-xvzhb7ix 98 8 fusion fusion NN cord-303381-xvzhb7ix 98 9 competence competence NN cord-303381-xvzhb7ix 98 10 Reemergence reemergence NN cord-303381-xvzhb7ix 98 11 of of IN cord-303381-xvzhb7ix 98 12 Ibaraki Ibaraki NNP cord-303381-xvzhb7ix 98 13 disease disease NN cord-303381-xvzhb7ix 98 14 in in IN cord-303381-xvzhb7ix 98 15 southern southern JJ cord-303381-xvzhb7ix 98 16 Japan Japan NNP cord-303381-xvzhb7ix 98 17 in in IN cord-303381-xvzhb7ix 98 18 2013 2013 CD cord-303381-xvzhb7ix 99 1 Dynasore Dynasore NNP cord-303381-xvzhb7ix 99 2 , , , cord-303381-xvzhb7ix 99 3 a a DT cord-303381-xvzhb7ix 99 4 cell cell NN cord-303381-xvzhb7ix 99 5 - - HYPH cord-303381-xvzhb7ix 99 6 permeable permeable JJ cord-303381-xvzhb7ix 99 7 inhibitor inhibitor NN cord-303381-xvzhb7ix 99 8 of of IN cord-303381-xvzhb7ix 99 9 dynamin dynamin NNP cord-303381-xvzhb7ix 99 10 Fusion Fusion NNP cord-303381-xvzhb7ix 99 11 of of IN cord-303381-xvzhb7ix 99 12 enveloped envelop VBN cord-303381-xvzhb7ix 99 13 viruses virus NNS cord-303381-xvzhb7ix 99 14 with with IN cord-303381-xvzhb7ix 99 15 cells cell NNS cord-303381-xvzhb7ix 99 16 and and CC cord-303381-xvzhb7ix 99 17 liposomes liposome NNS cord-303381-xvzhb7ix 99 18 . . . cord-303381-xvzhb7ix 100 1 Activity activity NN cord-303381-xvzhb7ix 100 2 and and CC cord-303381-xvzhb7ix 100 3 inactivation inactivation NN cord-303381-xvzhb7ix 100 4 R r NN cord-303381-xvzhb7ix 100 5 : : : cord-303381-xvzhb7ix 100 6 A a DT cord-303381-xvzhb7ix 100 7 language language NN cord-303381-xvzhb7ix 100 8 and and CC cord-303381-xvzhb7ix 100 9 environment environment NN cord-303381-xvzhb7ix 100 10 for for IN cord-303381-xvzhb7ix 100 11 statistical statistical JJ cord-303381-xvzhb7ix 100 12 computing computing NN cord-303381-xvzhb7ix 100 13 Acid acid NN cord-303381-xvzhb7ix 100 14 - - HYPH cord-303381-xvzhb7ix 100 15 induced induce VBN cord-303381-xvzhb7ix 100 16 membrane membrane NN cord-303381-xvzhb7ix 100 17 fusion fusion NN cord-303381-xvzhb7ix 100 18 by by IN cord-303381-xvzhb7ix 100 19 the the DT cord-303381-xvzhb7ix 100 20 hemagglutinin hemagglutinin NN cord-303381-xvzhb7ix 100 21 protein protein NN cord-303381-xvzhb7ix 100 22 and and CC cord-303381-xvzhb7ix 100 23 its -PRON- PRP$ cord-303381-xvzhb7ix 100 24 role role NN cord-303381-xvzhb7ix 100 25 in in IN cord-303381-xvzhb7ix 100 26 influenza influenza NN cord-303381-xvzhb7ix 100 27 virus virus NN cord-303381-xvzhb7ix 100 28 biology biology NN cord-303381-xvzhb7ix 100 29 Effects effect NNS cord-303381-xvzhb7ix 100 30 of of IN cord-303381-xvzhb7ix 100 31 low low JJ cord-303381-xvzhb7ix 100 32 pH ph NN cord-303381-xvzhb7ix 100 33 on on IN cord-303381-xvzhb7ix 100 34 influenza influenza NN cord-303381-xvzhb7ix 100 35 virus virus NN cord-303381-xvzhb7ix 100 36 . . . cord-303381-xvzhb7ix 101 1 Activation activation NN cord-303381-xvzhb7ix 101 2 and and CC cord-303381-xvzhb7ix 101 3 inactivation inactivation NN cord-303381-xvzhb7ix 101 4 of of IN cord-303381-xvzhb7ix 101 5 the the DT cord-303381-xvzhb7ix 101 6 membrane membrane NN cord-303381-xvzhb7ix 101 7 fusion fusion NN cord-303381-xvzhb7ix 101 8 capacity capacity NN cord-303381-xvzhb7ix 101 9 of of IN cord-303381-xvzhb7ix 101 10 the the DT cord-303381-xvzhb7ix 101 11 hemagglutinin hemagglutinin NN cord-303381-xvzhb7ix 101 12 Double Double NNP cord-303381-xvzhb7ix 101 13 - - HYPH cord-303381-xvzhb7ix 101 14 stranded strand VBN cord-303381-xvzhb7ix 101 15 RNA RNA NNP cord-303381-xvzhb7ix 101 16 of of IN cord-303381-xvzhb7ix 101 17 Ibaraki Ibaraki NNP cord-303381-xvzhb7ix 101 18 virus virus NN cord-303381-xvzhb7ix 101 19 Inhibition inhibition NN cord-303381-xvzhb7ix 101 20 of of IN cord-303381-xvzhb7ix 101 21 the the DT cord-303381-xvzhb7ix 101 22 vacuolar vacuolar JJ cord-303381-xvzhb7ix 101 23 H(+)-ATPase H(+)-ATPase NNP cord-303381-xvzhb7ix 101 24 with with IN cord-303381-xvzhb7ix 101 25 bafilomycin bafilomycin NNS cord-303381-xvzhb7ix 101 26 reduces reduce VBZ cord-303381-xvzhb7ix 101 27 delivery delivery NN cord-303381-xvzhb7ix 101 28 of of IN cord-303381-xvzhb7ix 101 29 internalized internalize VBN cord-303381-xvzhb7ix 101 30 molecules molecule NNS cord-303381-xvzhb7ix 101 31 from from IN cord-303381-xvzhb7ix 101 32 mature mature JJ cord-303381-xvzhb7ix 101 33 multivesicular multivesicular NNP cord-303381-xvzhb7ix 101 34 endosomes endosome VBZ cord-303381-xvzhb7ix 101 35 to to IN cord-303381-xvzhb7ix 101 36 lysosomes lysosome NNS cord-303381-xvzhb7ix 101 37 in in IN cord-303381-xvzhb7ix 101 38 HEp-2 HEp-2 NNP cord-303381-xvzhb7ix 101 39 cells cell NNS cord-303381-xvzhb7ix 101 40 Transport Transport NNP cord-303381-xvzhb7ix 101 41 from from IN cord-303381-xvzhb7ix 101 42 late late JJ cord-303381-xvzhb7ix 101 43 endosomes endosome NNS cord-303381-xvzhb7ix 101 44 to to IN cord-303381-xvzhb7ix 101 45 lysosomes lysosome NNS cord-303381-xvzhb7ix 101 46 , , , cord-303381-xvzhb7ix 101 47 but but CC cord-303381-xvzhb7ix 101 48 not not RB cord-303381-xvzhb7ix 101 49 sorting sort VBG cord-303381-xvzhb7ix 101 50 of of IN cord-303381-xvzhb7ix 101 51 integral integral JJ cord-303381-xvzhb7ix 101 52 membrane membrane NN cord-303381-xvzhb7ix 101 53 proteins protein NNS cord-303381-xvzhb7ix 101 54 in in IN cord-303381-xvzhb7ix 101 55 endosomes endosome NNS cord-303381-xvzhb7ix 101 56 , , , cord-303381-xvzhb7ix 101 57 depends depend VBZ cord-303381-xvzhb7ix 101 58 on on IN cord-303381-xvzhb7ix 101 59 the the DT cord-303381-xvzhb7ix 101 60 vacuolar vacuolar JJ cord-303381-xvzhb7ix 101 61 proton proton NN cord-303381-xvzhb7ix 101 62 pump pump VBP cord-303381-xvzhb7ix 101 63 The the DT cord-303381-xvzhb7ix 101 64 use use NN cord-303381-xvzhb7ix 101 65 of of IN cord-303381-xvzhb7ix 101 66 inhibitors inhibitor NNS cord-303381-xvzhb7ix 101 67 to to TO cord-303381-xvzhb7ix 101 68 study study VB cord-303381-xvzhb7ix 101 69 endocytic endocytic JJ cord-303381-xvzhb7ix 101 70 pathways pathway NNS cord-303381-xvzhb7ix 101 71 of of IN cord-303381-xvzhb7ix 101 72 gene gene NN cord-303381-xvzhb7ix 101 73 carriers carrier NNS cord-303381-xvzhb7ix 101 74 : : : cord-303381-xvzhb7ix 101 75 optimization optimization NN cord-303381-xvzhb7ix 101 76 and and CC cord-303381-xvzhb7ix 101 77 pitfalls pitfall NNS cord-303381-xvzhb7ix 101 78 Metabolic metabolic JJ cord-303381-xvzhb7ix 101 79 products product NNS cord-303381-xvzhb7ix 101 80 of of IN cord-303381-xvzhb7ix 101 81 microorganisms microorganism NNS cord-303381-xvzhb7ix 101 82 . . . cord-303381-xvzhb7ix 102 1 224 224 CD cord-303381-xvzhb7ix 102 2 . . . cord-303381-xvzhb7ix 103 1 Bafilomycins Bafilomycins NNP cord-303381-xvzhb7ix 103 2 , , , cord-303381-xvzhb7ix 103 3 a a DT cord-303381-xvzhb7ix 103 4 new new JJ cord-303381-xvzhb7ix 103 5 group group NN cord-303381-xvzhb7ix 103 6 of of IN cord-303381-xvzhb7ix 103 7 macrolide macrolide JJ cord-303381-xvzhb7ix 103 8 antibiotics antibiotic NNS cord-303381-xvzhb7ix 103 9 . . . cord-303381-xvzhb7ix 104 1 Production production NN cord-303381-xvzhb7ix 104 2 , , , cord-303381-xvzhb7ix 104 3 isolation isolation NN cord-303381-xvzhb7ix 104 4 , , , cord-303381-xvzhb7ix 104 5 chemical chemical NN cord-303381-xvzhb7ix 104 6 structure structure NN cord-303381-xvzhb7ix 104 7 and and CC cord-303381-xvzhb7ix 104 8 biological biological JJ cord-303381-xvzhb7ix 104 9 activity activity NN cord-303381-xvzhb7ix 104 10 Bluetongue Bluetongue NNP cord-303381-xvzhb7ix 104 11 virus virus NN cord-303381-xvzhb7ix 104 12 coat coat NN cord-303381-xvzhb7ix 104 13 protein protein NN cord-303381-xvzhb7ix 104 14 VP2 VP2 NNP cord-303381-xvzhb7ix 104 15 contains contain VBZ cord-303381-xvzhb7ix 104 16 sialic sialic JJ cord-303381-xvzhb7ix 104 17 acid acid NN cord-303381-xvzhb7ix 104 18 - - HYPH cord-303381-xvzhb7ix 104 19 binding bind VBG cord-303381-xvzhb7ix 104 20 domains domain NNS cord-303381-xvzhb7ix 104 21 , , , cord-303381-xvzhb7ix 104 22 and and CC cord-303381-xvzhb7ix 104 23 VP5 VP5 NNS cord-303381-xvzhb7ix 104 24 resembles resemble VBZ cord-303381-xvzhb7ix 104 25 enveloped envelop VBN cord-303381-xvzhb7ix 104 26 virus virus NN cord-303381-xvzhb7ix 104 27 fusion fusion NN cord-303381-xvzhb7ix 104 28 proteins protein NNS cord-303381-xvzhb7ix 104 29 ACKNOWLEDGMENTS acknowledgment NNS cord-303381-xvzhb7ix 104 30 . . . cord-303381-xvzhb7ix 105 1 We -PRON- PRP cord-303381-xvzhb7ix 105 2 thank thank VBP cord-303381-xvzhb7ix 105 3 members member NNS cord-303381-xvzhb7ix 105 4 in in IN cord-303381-xvzhb7ix 105 5 the the DT cord-303381-xvzhb7ix 105 6 laboratory laboratory NN cord-303381-xvzhb7ix 105 7 of of IN cord-303381-xvzhb7ix 105 8 veterinary veterinary JJ cord-303381-xvzhb7ix 105 9 hygiene hygiene NN cord-303381-xvzhb7ix 105 10 and and CC cord-303381-xvzhb7ix 105 11 Dr. Dr. NNP cord-303381-xvzhb7ix 105 12 Brendan Brendan NNP cord-303381-xvzhb7ix 105 13 T. T. NNP cord-303381-xvzhb7ix 105 14 Higgins Higgins NNP cord-303381-xvzhb7ix 105 15 for for IN cord-303381-xvzhb7ix 105 16 helpful helpful JJ cord-303381-xvzhb7ix 105 17 comments comment NNS cord-303381-xvzhb7ix 105 18 and and CC cord-303381-xvzhb7ix 105 19 critical critical JJ cord-303381-xvzhb7ix 105 20 reading reading NN cord-303381-xvzhb7ix 105 21 of of IN cord-303381-xvzhb7ix 105 22 the the DT cord-303381-xvzhb7ix 105 23 manuscript manuscript NN cord-303381-xvzhb7ix 105 24 . . . cord-303381-xvzhb7ix 106 1 This this DT cord-303381-xvzhb7ix 106 2 research research NN cord-303381-xvzhb7ix 106 3 was be VBD cord-303381-xvzhb7ix 106 4 supported support VBN cord-303381-xvzhb7ix 106 5 in in IN cord-303381-xvzhb7ix 106 6 part part NN cord-303381-xvzhb7ix 106 7 by by IN cord-303381-xvzhb7ix 106 8 Research research NN cord-303381-xvzhb7ix 106 9 aid aid NN cord-303381-xvzhb7ix 106 10 from from IN cord-303381-xvzhb7ix 106 11 Yamaguchi Yamaguchi NNP cord-303381-xvzhb7ix 106 12 University University NNP cord-303381-xvzhb7ix 106 13 Foundation Foundation NNP cord-303381-xvzhb7ix 106 14 and and CC cord-303381-xvzhb7ix 106 15 Yamaguchi Yamaguchi NNP cord-303381-xvzhb7ix 106 16 University University NNP cord-303381-xvzhb7ix 106 17 Fellowships Fellowships NNPS cord-303381-xvzhb7ix 106 18 for for IN cord-303381-xvzhb7ix 106 19 Young Young NNP cord-303381-xvzhb7ix 106 20 Researchers Researchers NNPS cord-303381-xvzhb7ix 106 21 . . .