id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003328-vvle1q1e	Altawaty, Tawfeek	Lack of LTβR Increases Susceptibility of IPEC-J2 Cells to Porcine Epidemic Diarrhea Virus	2018-11-21		.txt	text/plain	4114	221	53	To examine whether CRISPR/Cas9-mediated gene editing could generate LTβR null alleles in IPEC-J2 cells, we randomly selected two biallelic mutation clones (1-10# and 1-22#) and compared their LTβR expression levels to those of wild-type IPEC-J2 cells (hereafter designated LTβR +/+ ) by real-time PCR. Since PEDV infection destroys epithelial barrier integrity [22] , and LTβR signaling was reported to limit mucosal damage through the IL-22-IL-23 pathway [10] , we examined the expression levels of LTβR downstream genes by semi-quantitative PCR, including VCAM1, IL-22 and IL-23, in PEDV-infected cells. We detected expression levels of IL-6 and IL-8 in infected cells, and the data showed that both genes were significantly decreased in infected LTβR −/− IPEC-J2 cells ( Figure 5C ). Since it has been demonstrated that PEDV infection destroys epithelial barrier integrity [22] and LTβR signaling limits mucosal damage through the IL-22-IL-23 pathway [10] , we detected expression levels of VCAM1, IL-22 and IL-23 in both cell types by semi-quantitative PCR.	./cache/cord-003328-vvle1q1e.txt	./txt/cord-003328-vvle1q1e.txt