id author title date pages extension mime words sentences flesch summary cache txt cord-306502-jkqg1qal Dee, Scott An evaluation of contaminated complete feed as a vehicle for porcine epidemic diarrhea virus infection of naïve pigs following consumption via natural feeding behavior: proof of concept 2014-08-05 .txt text/plain 3750 189 56 title: An evaluation of contaminated complete feed as a vehicle for porcine epidemic diarrhea virus infection of naïve pigs following consumption via natural feeding behavior: proof of concept Here we provide proof of concept of this risk by describing a novel means for recovering PEDV-contaminated complete feed material from commercial swine sites and conducting an in vivo experiment to prove its infectivity. Clinical signs of PEDV infection (vomiting and diarrhea) and viral shedding were observed in both the Positive control and Treatment group' post-consumption with virus and microscopic lesions detected in intestinal samples No evidence of infection was observed in the Negative controls. As more data regarding the risk of PEDV transmission via complete feed are needed, we conducted a study to test the risk of PEDV-contaminated complete feed using a novel on-farm sampling method for virus detection in feed along with an in vivo experiment (swine bioassay) using at-risk feed material. ./cache/cord-306502-jkqg1qal.txt ./txt/cord-306502-jkqg1qal.txt