id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-339546-m7rqr886	de Arriba, M.L	Isotype-specific antibody-secreting cells in systemic and mucosal associated lymphoid tissues and antibody responses in serum of conventional pigs inoculated with PEDV	2002-01-01		.txt	text/plain	6465	321	57	An enzyme-linked immunospot (ELISPOT) has been developed to detect porcine epidemic diarrhea virus (PEDV)-specific antibody secreting cells (ASC) in gut associated lymphoid tissues (duodenum and ileum lamina propria and mesenteric lymph nodes) and systemic locations (spleen and blood) of conventional pigs so as to characterise the mucosal and systemic antibody response generated by the infection with PEDV. Memory antibody response to the PEDV was also studied by secondary in vitro stimulation of the mononuclear cells (MNC) isolated from mesenteric lymph nodes, spleen and blood. In order to evaluate the distribution of PEDV-speci®c ASC, MNC from mesenteric lymph nodes, lamina propria of duodenum and ileum, spleen and blood were recovered at various PID from PEDV exposed pigs and tested for antibody production by ELISPOT. Table 1 Numbers of isotype-specific ASC to PEDV (per 5 Â 10 5 MNC) in duodenum and ileum lamina propria, mesenteric lymph nodes (MLN), spleen and blood from pigs experimentally exposed to the virulent isolate of the PEDV strain CV-777 and sacrificed on PID 4, 7, 14, 21, 25 	./cache/cord-339546-m7rqr886.txt	./txt/cord-339546-m7rqr886.txt