id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-341521-dntkdwkj	Luo, Yi-Ran	Porcine Epidemic Diarrhoea Virus Induces Cell-cycle Arrest through the DNA Damage-signalling Pathway	2020-03-24		.txt	text/plain	4077	168	52	MATERIAL AND METHODS: We observed the effect of the PEDV SHpd/2012 strain on an infected Vero cell cycle through flow cytometry and Western blot, investigating the interrelationships of cell-cycle arrest, the DNA damage–signalling pathway caused by PEDV and the phosphorylation levels of the key molecules Chk.2 and H2A.X involved upstream and downstream in this pathway. We used inhibitors of ATM and Chk.2 to block the DNA damage-signalling pathway and to confirm whether PEDV infection can lead to cell-cycle arrest. We also treated cells with chemical inhibitors of ATM and Chk.2 to determine if cell-cycle arrest after PEDV infection was linked with activation of the DNA damage-signalling pathway. These results revealed that cell-cycle arrest after infection of Vero cells with PEDV was caused by activation of the DNA damage-signalling pathway.	./cache/cord-341521-dntkdwkj.txt	./txt/cord-341521-dntkdwkj.txt