id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-344558-1jgqofbr	Kocherhans, Rolf	Completion of the Porcine Epidemic Diarrhoea Coronavirus (PEDV) Genome Sequence	2001		.txt	text/plain	2839	159	63	A cloning strategy, which involves primers based on conserved regions in the predicted ORF1 products from other coronaviruses whose genome sequence has been determined, was used to amplify the equivalent, but as yet unknown, sequence of PEDV. Comparative amino acid sequence alignments revealed that PEDV is most closely related to human coronavirus (HCoV)-229E and transmissible gastroenteritis virus (TGEV) and less related to murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). The alignment of the deduced amino acid sequences indicated that PEDV occupies an interesting intermediate position between the two well-characterized members of the group I coronaviruses, transmissible gastroenteritis virus (TGEV) and human coronavirus (HCoV)-229E. In order to obtain the first partial PEDV specific sequences, the predicted amino acid sequences of the HCoV-229E and TGEV polymerase ORFs were aligned and homologous regions identified. The deduced amino acid sequences of ORF1a and ORF1b from PEDV were aligned with the corresponding sequences of HCoV-229E, TGEV, MHV-A59, and IBV using PILEUP.	./cache/cord-344558-1jgqofbr.txt	./txt/cord-344558-1jgqofbr.txt