id author title date pages extension mime words sentences flesch summary cache txt cord-010681-tmpxs9og Dondapati, Srujan Kumar Cell-Free Protein Synthesis: A Promising Option for Future Drug Development 2020-03-20 .txt text/plain 10674 487 36 aatRNA aminoacyl-tRNA, AAS aminoacyl-tRNA synthetase, ATP adenosine triphosphate, EF elongation factor, GSH glutathione, GSSG glutathione-disulfide, GTP guanosine-5'-triphosphate, IF initiation factor, IRES internal ribosome entry site, MP membrane protein, nCAA non-canonical amino acid, PDI protein disulfide isomerase, PEG polyethylene glycol, PTM post-translational modification, R ribosomes, t-RNA transfer RNA, TF transcription factor, UTR untranslated region, VLP virus like particle Chinese hamster ovary (CHO) Mimic the CHO cell-based production PTMs (N-glycosylation, disulfide bridging, and lipidation) Suitable for a wide range of eukaryotic and complex proteins Presence of translational active endogenous microsomes [45] High yields in CECF mode Endotoxin free Lysates used for point-of-care testing [30] Low yields especially in the batch mode [58] Cost ineffective and difficult to establish unlike E. ./cache/cord-010681-tmpxs9og.txt ./txt/cord-010681-tmpxs9og.txt