id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-252576-1ec545o2	Wu, Xiangli	An antifungal defensin from Phaseolus vulgaris cv. ‘Cloud Bean’	2011-01-15		.txt	text/plain	3562	195	55	An antifungal peptide with a defensin-like sequence and exhibiting a molecular mass of 7.3 kDa was purified from dried seeds of Phaseolus vulgaris 'Cloud Bean'. After elution of unadsorbed proteins (fraction S1), adsorbed proteins were desorbed with a linear concentration (0-1 M) gradient of NaCl. The fraction with antifungal activity (S2) was then further fractionated by fast protein liquid chromatography on a Superdex 75 HR 10/30 column (GE Healthcare) in 0.2 M NH 4 HCO 3 buffer (pH 8.5). Antiproliferative activity toward tumor cells is also an attribute of defensins (Wong and Ng 2003) , defensin-like peptides and also other antifungal proteins including ribosome inactivating proteins (Lam et al. Some antifungal proteins including defensin-like peptides (Wong and Ng 2003) , protease inhibitors (Ye et al. The present findings on cloud bean defensin is reminiscent of the observation that mungin, an antifungal protein from mung beans, is without HIV-1 reverse transcriptase inhibitory activity (Ye and Ng 2000) . Concurrent purification of two defense proteins from French bean seeds: a defensin-like antifungal peptide and a hemagglutinin	./cache/cord-252576-1ec545o2.txt	./txt/cord-252576-1ec545o2.txt