id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-254909-8zgvovu4	Srivastava, Rajneesh	Serum profiling of leptospirosis patients to investigate proteomic alterations()	2012-12-05		.txt	text/plain	5745	245	29	In this study, we have performed a proteomic analysis of serum samples from leptospirosis patients (n = 6), febrile controls (falciparum malaria) (n = 8) and healthy subjects (n = 18) to obtain an insight about disease pathogenesis and host immune responses in leptospiral infections. During the database search following parameters were specified: human taxonomy, trypsin digestion with one missed cleavage, carbamidomethyl (C) as fixed modification, oxidation (M) as variable modification, peptide mass tolerance set at 75 ppm and MS/MS tolerance of 0.4 Da. Western blot analysis was performed with serum samples from controls (healthy and febrile) and leptospirosis patients (n = 6) to validate the differential expression of some of the target proteins identified in 2DE and 2D-DIGE experiments. Aiming at analysis of host serum proteome alteration due to leptospirosis, we have identified several differentially expressed proteins and modulation of multiple physiological processes and pathways, including inflammation mediated acute phase responses, complement pathways, heterotrimeric G-protein signaling pathway, coagulation cascade and hemostasis in patients suffering from leptospiral infection.	./cache/cord-254909-8zgvovu4.txt	./txt/cord-254909-8zgvovu4.txt