id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-260440-e63pgcir	Dinjaski, Nina	Smart polyhydroxyalkanoate nanobeads by protein based functionalization	2015-02-24		.txt	text/plain	9066	520	35	In vivo PHA modification based on peptide functionalization of PHA nano-beads using GAPs for recombinant protein anchoring to the PHA granule or nonspecific binding and in vivo chemical modification through incorporation of functional group in the side chain of the polymer applying metabolic engineering and systems biology approach. 30 The main advantages of this in vitro cell-free system are: i) the possibility of tight control of nanoparticle disassembly and reassembly process; ii) absence of competition among the recombinant GAP-fusion and wild type proteins; iii) tight control over particle size and immobilized protein/active agent concentration; iv) possibility of endotoxin removal, crucial for the design of every biomedical setup. 18 In completely different context to in vivo tag binding, in vitro synthesized PHA nanoparticles and in vitro hydrophobic binding of PhaP fusion proteins with protein ligands (e.g., mannosylated human α1-acid glycoprotein (hAGP) and human epidermal growth factor (hEGF)) have been reported as another outstanding application of phasins for receptor-mediated drug delivery.	./cache/cord-260440-e63pgcir.txt	./txt/cord-260440-e63pgcir.txt