id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261472-qcu73sdu	Yao, Yong Xiu	Cleavage and Serum Reactivity of the Severe Acute Respiratory Syndrome Coronavirus Spike Protein	2004-07-01		.txt	text/plain	3851	159	47	Severe acute respiratory syndrome (SARS) coronavirus (SCoV) spike (S) protein is the major surface antigen of the virus and is responsible for receptor binding and the generation of neutralizing antibody. To investigate SCoV S protein, full-length and individual domains of S protein were expressed on the surface of insect cells and were characterized for cleavability and reactivity with serum samples obtained from patients during the convalescent phase of SARS. The possible use of insect cell-displayed S protein for diagnostic application was assessed by examining fragment reactivity with serum samples from patients infected with human CoV 229E and also with serum from a patient with suspected but clinically unconfirmed SARS (serum sample 3118). Of the 2 assay formats we used, nondenatured S protein present on the cell surface provided the most sensitive detection of antibodies, with clear shifts in fluorescence for serum samples from patients with suspected but clinically unconfirmed SARS.	./cache/cord-261472-qcu73sdu.txt	./txt/cord-261472-qcu73sdu.txt